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  • 1.
    Adrian, Monica Lovestam
    et al.
    Lund Univ, Dept Ophthalmol, SE-22185 Lund, Sweden.
    Vassilev, Zdravko P.
    Bayer US, Whippany, NJ USA.
    Westborg, Inger
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Baseline visual acuity as a prognostic factor for visual outcomes in patients treated with aflibercept for wet age-related macular degeneration: data from the INSIGHT study using the Swedish Macula Register2019Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 97, nr 1, s. 91-98Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose: To assess mean change in visual acuity (VA) overall and stratified by baseline VA after 1 and 2 years' treatment with aflibercept in a real-life setting.

    Methods: This was an observational cohort study using nationwide data from the Swedish Macula Register. Treatment-naive patient eyes with wet age-related macular degeneration and prescribed aflibercept from January 2013 to December 2014 were followed for 1 year (2478 eyes) or 2 years (831 eyes) to assess VA. Eyes were grouped by baseline VA.

    Results: Mean number of injections in patients treated according to label (72%) versus patients treated not according to label was 8.0 +/- 1.5 versus 4.4 +/- 0.8 (p < 0.0001) at 1 year, and 12.5 +/- 3.2 versus 7.3 +/- 1.9 (p < 0.0001) at 2 years. Among all eyes, mean VA increased from 61.3 +/- 13.4 Early Treatment Diabetic Retinopathy Study letters at baseline to 64.5 +/- 15.6 at 1 year and 65.1 +/- 15.1 letters at 2 years. At 2 years, eyes with good baseline vision (>= 70 letters) lost a mean of 2.4 +/- 11.3 to 72.3 letters, eyes with intermediate baseline VA (36-69 letters) gained 5.7 +/- 14.1 to 62.7 letters, and eyes with poor baseline VA (<= 35 letters) gained 13.2 +/- 18.3 to 41.0 letters. Also at 2 years, 75% of treated eyes were stable or had improved VA. Among eyes with intermediate baseline VA, near vision was significantly better among those treated according to label versus not according to label at 3 (p = 0.019), 6 (p = 0.0002) and 12 months (p <= 0.0001).

    Conclusion: While gain in vision was especially pronounced in eyes with poor baseline VA, good baseline VA was important for best prognosis.

  • 2.
    Alm, A.
    et al.
    Uppsala universitet, Medicinska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap. Uppsala universitet, Medicinska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap. Oftalmiatrik.
    Schoenfelder, J.
    McDermott, J.
    A 5-year, multicenter, open-label, safety study of adjunctive latanoprost therapy for glaucoma.2004Ingår i: Arch Ophthalmol, Vol. 122, s. 957-965Artikel i tidskrift (Refereegranskat)
  • 3.
    Alm, Albert
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Wikström, Carl Peter
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Ekström, Curt
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Öhman, Lena
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    The effect of metoprolol on intra-ocular pressure in glaucoma. A pilot study.1979Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 57, nr 2, s. 236-242, artikel-id j.1755-3768.1979.tb00487.xArtikel i tidskrift (Refereegranskat)
  • 4.
    An, Hongbin
    et al.
    HUST, Sch Mech Sci & Engn, Wuhan 430074, Hubei, Peoples R China.
    Chen, Liangzhou
    HUST, Sch Mech Sci & Engn, Wuhan 430074, Hubei, Peoples R China.
    Liu, Xiaojun
    HUST, Sch Mech Sci & Engn, Wuhan 430074, Hubei, Peoples R China.
    Zhao, Bin
    HUST, Sch Mech Sci & Engn, Wuhan 430074, Hubei, Peoples R China.
    Zhang, Hong
    HUST, Tongji Med Coll, Tongji Hosp, Dept Ophthalmol, Wuhan 430074, Hubei, Peoples R China.
    Wu, Zhigang
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Mikrosystemteknik. HUST, Sch Mech Sci & Engn, Wuhan 430074, Hubei, Peoples R China.
    Microfluidic contact lenses for unpowered, continuous and non-invasive intraocular pressure monitoring2019Ingår i: Sensors and Actuators A-Physical, ISSN 0924-4247, E-ISSN 1873-3069, Vol. 295, s. 177-187Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Intraocular pressure (IOP) is a crucial physiological indicator of the visual system and play a key role in the diagnosis and treatment of glaucoma. However, the current handheld single measurement tools for IOP sensing cannot meet the future demands for glaucoma management. Thus, here we present the microfluidic contact lens sensors that could provide unpowered, continuous and non-invasive IOP monitoring. The microfluidic contact lens is comprised of a sensing layer of the micropatterned soft-elastomer and a hard plastic reference layer. The devices use the annular sensing chamber filled with the dyed liquid and a sensing microchannel as the IOP transducer. Resulting from the volume variance of the sensing chamber and caused by the deformation of the sensing layer under pressure, the IOP signal is detected as the displacement change of the dyed liquid's interface in the sensing channel, and in which, the displacement change can be optically observed by using the smart-phone camera. Based on the silicone rubber model eyeball, the sensing mechanism of the devices with different design parameters (the position of the sensing chambers and the dimension of the sensing channels) are explored by using the theoretical analyses and experimental investigations. The characteristics of these microfluidic contact lens sensors are tested, in which, the maximum sensitivity of the device (with the sensing chamber of 8.5 mm in diameter and the sensing channel of 100 x 40 um in size) can be achieved to 0.708 mm/mmHg in a working range of 0 (4) over tilde0 mmHg. Also, cyclical tests were conducted and indicated that the devices had a good reversibility and Long-term stability. Furthermore, the device (with the sensing chamber of 5.0 mm in diameter and the sensing channel of 150 x 40 urn in size) was test on the porcine eyes ex vivo, showing a sensitivity of 0.2832 mm/mmHg in a range of 8 (3) over tilde2 mmHg and, the device had a good reproducibility to its IOP change. This work provides a promising approach for unpowered, continuous and non-invasive monitoring of IOP.

  • 5.
    Aurell, Sandra
    et al.
    Vastmanland Cty Hosp, AT Ctr, S-72189 Vasteras, Sweden..
    Granstam, Elisabet
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Centrum för klinisk forskning, Västerås. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik. Vastmanland Cty Hosp, Dept Ophthalmol, Vasteras, Sweden..
    Bacterial keratitis in a Swedish county hospital: management and clinical outcome2016Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 94, nr 3, s. E248-E249Artikel i tidskrift (Refereegranskat)
  • 6.
    Aurell, Sandra
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Centrum för klinisk forskning, Västerås. Hosp Vastmanland, Dept Ophthalmol, Vasteras, Sweden.
    Sjövall, Kersti
    Hosp Vastmanland, Dept Ophthalmol, Vasteras, Sweden.
    Paul, Anna
    Hosp Vastmanland, Dept Ophthalmol, Vasteras, Sweden.
    Morén, Åsa
    Hosp Vastmanland, Dept Ophthalmol, Vasteras, Sweden.
    Granstam, Elisabet
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Centrum för klinisk forskning, Västerås. Hosp Vastmanland, Dept Ophthalmol, Vasteras, Sweden.
    Better visual outcome at 1 year with antivascular endothelial growth factor treatment according to treat-and-extend compared with pro re nata in eyes with neovascular age-related macular degeneration2019Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 97, nr 5, s. 519-524Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose: To evaluate treatment outcome at 12 months in eyes with neovascular age-related macular degeneration (nAMD) treated with antivascular endothelial growth factor (anti-VEGF) injections according to either pro re nata (PRN)- or treat-and-extend (TE)-regimen in one clinical setting in Sweden.

    Methods: Data were obtained retrospectively from the Swedish Macula Register, optical coherence tomography-database and electronic patient charts. The study included 443 eyes; 223 PRN- and 220 TE-treated eyes. Baseline (BL) characteristics and follow-up data at 6 and 12 months were collected. Statistical regression analysis was performed to evaluate association between treatment strategy and visual outcome at 12 months.

    Results: Baseline (BL) characteristics were well balanced between cohorts. Visual acuity at 12 months was higher in TE-cohort 66.5 (13.1) compared to PRN-cohort 60.1 (17.6) (p = 0.000). Visual improvement at 12 months was +5.2 (11.8) and +1.2 (12.7) letters Early Treatment Diabetic Retinopathy Study (ETDRS) in TE- and PRN-cohorts, respectively (p = 0.002). Number of administered injections at 12 months was 10.2 (2.1) and 6.3 (2.1) in the two cohorts (p = 0.000). Statistical analysis demonstrated a strong association between TE treatment strategy and improvement in visual acuity at 12 months.

    Conclusion: Eyes treated according to TE had better visual outcome at 12 months. The results indicate that treatment according to proactive TE-regimen is superior to treatment according to PRN-regimen in clinical routine care of nAMD.

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  • 7.
    Austeng, Dordi
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Retinopathy of Prematurity in Infants Born Before 27 Weeks of Gestation: A National Population-based Study in Sweden During 2004-20072010Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Background: Improved neonatal care has resulted in an increasing population of surviving infants. Neonatal morbidity in preterm infants is, however, high, and retinopathy of prematurity (ROP) is one of the major neonatal morbidities. Observations have suggested that ROP might have a different course in extremely preterm compared to more mature infants.

    Aims: To study the incidence, natural history and treatment of the disease, and the implications regarding screening recommendations for the population of extremely preterm infants.

    Methods: A national, population-based study of neonatal morbidity in infants born before 27 gestational weeks was performed in Sweden during 2004 to 2007. ROP screening started in the 5th postnatal week and continued until the retina was completely vascularized.

    Results: Of the 506 infants surviving until the first ROP examination, 73% developed ROP; 38% mild ROP and 35% severe ROP. Ninety-nine infants (20%) were treated. A log-linear relationship was found between severe ROP and gestational age (GA) at birth, and the risk of ROP was reduced by 50% for each week of increase in GA at birth (Paper I).

    Postmenstrual age (PMA) at onset of ROP was significantly related to GA at birth, as was the site of onset of ROP. ROP had a predilection to start in the nasal retina in the most immature infants. There were significant relations between PMA at onset of ROP and severity of ROP as well as between the site of onset of ROP and severe ROP (Paper III).

    The most immature infants had a higher risk of reaching treatment criteria for ROP, a higher risk of progression from ROP 3 to treatment criteria, and they reached these criteria at an earlier PMA than the less immature infants (Paper II).

    According to our results, the first examination can be postponed until a PMA of 31 weeks in infants born before 27 weeks of gestation, since onset of ROP 3 did not occur before this age, and criteria for treatment were not reached before 32 weeks. The majority of infants (75%) were treated during a limited period, i.e. before a PMA of 39 weeks (Paper IV).

    Delarbeten
    1. Incidence of retinopathy of prematurity in infants born before 27 weeks' gestation in Sweden
    Öppna denna publikation i ny flik eller fönster >>Incidence of retinopathy of prematurity in infants born before 27 weeks' gestation in Sweden
    Visa övriga...
    2009 (Engelska)Ingår i: Archives of ophthalmology (1960), ISSN 0003-9950, Vol. 127, nr 10, s. 1315-1319Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    OBJECTIVE: To determine the incidence of retinopathy of prematurity (ROP) in extremely preterm infants born before 27 weeks' gestation in Sweden during a 3-year period. METHODS: A national, prospective, population-based study was performed in Sweden from April 1, 2004, to March 31, 2007. The ophthalmologic part of the study was separately organized, and screening for ROP was performed beginning postnatal week 5. The criteria for the treatment of ROP agreed with the recommendations of the Early Treatment for Retinopathy of Prematurity Cooperative Group. RESULTS: During the study, 506 of 707 live-born infants survived until the first eye examination. Of these, 368 (72.7%) had ROP: 37.9% had mild ROP and 34.8% had severe ROP. Ninety-nine infants (19.6%) were treated. Gestational age at birth was a stronger predictor of ROP than was birth weight. A log-linear relationship between severe ROP and gestational age at birth was found in the present cohort, and the risk of ROP was reduced by 50% for each week of increase in gestational age at birth. CONCLUSIONS: Today, extremely preterm infants are surviving, and this population-based study with ROP as a primary outcome shows a higher incidence of this condition than in previously reported national cohorts.

    Nationell ämneskategori
    Medicin och hälsovetenskap
    Identifikatorer
    urn:nbn:se:uu:diva-121732 (URN)10.1001/archophthalmol.2009.244 (DOI)000287329500008 ()19822848 (PubMedID)
    Tillgänglig från: 2010-03-29 Skapad: 2010-03-29 Senast uppdaterad: 2017-12-12Bibliografiskt granskad
    2. Treatment for retinopathy of prematurity in infants born before 27 weeks of gestation in Sweden
    Öppna denna publikation i ny flik eller fönster >>Treatment for retinopathy of prematurity in infants born before 27 weeks of gestation in Sweden
    Visa övriga...
    2010 (Engelska)Ingår i: British Journal of Ophthalmology, ISSN 0007-1161, E-ISSN 1468-2079, Vol. 94, nr 9, s. 1136-1139Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    AIMS: To study various aspects of treatment for retinopathy of prematurity (ROP) in a Swedish population of extremely preterm infants born before 27 weeks of gestation. METHODS: A national, prospective and population-based study was performed in Sweden from April 1, 2004 to March 31, 2007. The criteria for treatment of ROP accorded with the recommendations of the Early Treatment for Retinopathy of Prematurity Cooperative Group. RESULTS: Twenty percent of the infants (99/506) were treated for ROP. The likelihood of reaching treatment criteria nearly doubled for each week of reduction in gestational age (GA) at birth. The first treatment was performed at an earlier postmenstrual age in the most immature infants. One third of the infants had more than one session of laser treatment. CONCLUSIONS: A high percentage of these extremely preterm infants required treatment for ROP. The likelihood of reaching treatment criteria increased with a decline in GA at birth. Although only a few infants progressed to ROP Stages 4 and 5, our findings indicate a potential for improvement of the treatment routines, both regarding timing and number of laser spots at the first treatment.

    Nyckelord
    Retinopathy of prematurity, extremely preterm, population-based, treatment
    Nationell ämneskategori
    Oftalmologi
    Forskningsämne
    Oftalmiatrik; Pediatrik
    Identifikatorer
    urn:nbn:se:uu:diva-121731 (URN)10.1136/bjo.2009.170704 (DOI)000282206500006 ()19951941 (PubMedID)
    Tillgänglig från: 2010-03-29 Skapad: 2010-03-29 Senast uppdaterad: 2017-12-12Bibliografiskt granskad
    3. Natural history of retinopathy of prematurity in infants born before 27 weeks' gestation in Sweden
    Öppna denna publikation i ny flik eller fönster >>Natural history of retinopathy of prematurity in infants born before 27 weeks' gestation in Sweden
    Visa övriga...
    2010 (Engelska)Ingår i: Archives of ophthalmology (1960), ISSN 0003-9950, Vol. 128, nr 10, s. 1289-1294Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Objective: To investigate the natural history of retinopathy of prematurity (ROP) in 506 extremely preterm infants born before 27 weeks' gestation in Sweden during a 3-year period.

    Methods: A national population–based study was performed in Sweden from April 1, 2004, to March 31, 2007. According to the study protocol, initial eye examinations were to be performed at postnatal week 5, and examinations were repeated until the retina was completely vascularized or until criteria for treatment were met. The examinations were to be performed weekly, enabling study of the course and severity of ROP. In infants without ROP or with mild ROP without progression during the latest examinations, further examinations were performed weekly or every other week from postmenstrual age 35 weeks.

    Results: During the study, 368 infants (72.7%) developed ROP. Postmenstrual age at onset of ROP was significantly related to severity of ROP, even when controlling for gestational age (ie, the earlier the onset of ROP, the higher the risk of developing severe ROP). Site of onset of ROP was significantly related to gestational age at birth. The risk of nasal onset was almost doubled for every week of decrease in gestational age at birth. Nasal onset was associated with severe ROP, even after adjusting for gestational age at birth.

    Conclusion: This population-based study confirms results of the Cryotherapy for Retinopathy of Prematurity study and shows new correlations regarding time and site of onset of ROP, both of which are associated with disease severity.

    Ort, förlag, år, upplaga, sidor
    American Medical Association, 2010
    Nyckelord
    Retinopathy of prematurity, extremely preterm, population-based, natural history
    Nationell ämneskategori
    Oftalmologi
    Forskningsämne
    Oftalmiatrik
    Identifikatorer
    urn:nbn:se:uu:diva-122983 (URN)10.1001/archophthalmol.2010.234 (DOI)000282844000007 ()
    Tillgänglig från: 2010-04-22 Skapad: 2010-04-22 Senast uppdaterad: 2017-12-12Bibliografiskt granskad
    4.
    Posten kunde inte hittas. Det kan bero på att posten inte längre är tillgänglig eller att du har råkat ange ett felaktigt id i adressfältet.
    Ladda ner fulltext (pdf)
    FULLTEXT01
  • 8.
    Austeng, Dordi
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Källen, Karin B. M.
    Lund University.
    Ewald, Uwe W.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kvinnors och barns hälsa.
    Wallin, Agneta
    St.Erik's Eye Hospital.
    Holmström, Gerd E.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Treatment for retinopathy of prematurity in infants born before 27 weeks of gestation in Sweden2010Ingår i: British Journal of Ophthalmology, ISSN 0007-1161, E-ISSN 1468-2079, Vol. 94, nr 9, s. 1136-1139Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    AIMS: To study various aspects of treatment for retinopathy of prematurity (ROP) in a Swedish population of extremely preterm infants born before 27 weeks of gestation. METHODS: A national, prospective and population-based study was performed in Sweden from April 1, 2004 to March 31, 2007. The criteria for treatment of ROP accorded with the recommendations of the Early Treatment for Retinopathy of Prematurity Cooperative Group. RESULTS: Twenty percent of the infants (99/506) were treated for ROP. The likelihood of reaching treatment criteria nearly doubled for each week of reduction in gestational age (GA) at birth. The first treatment was performed at an earlier postmenstrual age in the most immature infants. One third of the infants had more than one session of laser treatment. CONCLUSIONS: A high percentage of these extremely preterm infants required treatment for ROP. The likelihood of reaching treatment criteria increased with a decline in GA at birth. Although only a few infants progressed to ROP Stages 4 and 5, our findings indicate a potential for improvement of the treatment routines, both regarding timing and number of laser spots at the first treatment.

    Ladda ner fulltext (pdf)
    FULLTEXT01
  • 9.
    Austeng, Dordi
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Källen, Karin
    Hellström, Ann
    Jakobsson, Peter
    Lundgren, Pia
    Tornqvist, Kristina
    Wallin, Agneta
    Holmström, Gerd
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Regional differences in screening for retinopathy of prematurity in infants born before 27 weeks' gestation in Sweden: the EXPRESS study2014Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 92, nr 4, s. 311-315Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose:  The primary aim was to analyse regional incidences of retinopathy of prematurity (ROP) and frequencies of treatment and their relation to perinatal risk factors during a 3-year period. A secondary aim was to study adherence to the study screening protocol in the different regions.

    Methods:  A population-based study of neonatal morbidity in extremely preterm infants in Sweden (EXPRESS) was performed during 2004–2007. Screening for ROP was to start at postnatal age 5 weeks and to continue weekly until the retina was completely vascularized or until regression of ROP. Logistic regression analyses were used for evaluation of differences in incidence of Any ROP, ROP 3 or more and ROP Type 1 between the seven regions of the country.

    Results:  The regional incidence of ROP varied between 54% and 92% for Any ROP, between 25% and 43% for ROP stage 3 or more and between 8% and 23% of infants with ROP Type 1, all of whom were treated. There was no significant difference between the regions regarding ROP Type 1, even when adjusting for known risk factors for ROP.

    Conclusion:  The heterogeneity between the regions regarding the incidence of ROP was reduced with increasing severity of ROP, and there was no heterogeneity regarding frequency of treatment for ROP, which is the most important issue for the children. We cannot exclude observer bias regarding mild ROP and ROP stage 3 in this study.

  • 10.
    Austeng, Dordi
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Källen, Karin
    Tornbladsinstitutet, Lund University.
    Hellström, Ann
    Section of Pediatric Ophthalmology, The Queen Silvia Children's Hospital, Sahlgrenska Academy, University of Gothenburg .
    Tornqvist, Kristina
    Department of Ophthalmology, Lund University Hospital.
    Holmström, Gerd E.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Natural history of retinopathy of prematurity in infants born before 27 weeks' gestation in Sweden2010Ingår i: Archives of ophthalmology (1960), ISSN 0003-9950, Vol. 128, nr 10, s. 1289-1294Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Objective: To investigate the natural history of retinopathy of prematurity (ROP) in 506 extremely preterm infants born before 27 weeks' gestation in Sweden during a 3-year period.

    Methods: A national population–based study was performed in Sweden from April 1, 2004, to March 31, 2007. According to the study protocol, initial eye examinations were to be performed at postnatal week 5, and examinations were repeated until the retina was completely vascularized or until criteria for treatment were met. The examinations were to be performed weekly, enabling study of the course and severity of ROP. In infants without ROP or with mild ROP without progression during the latest examinations, further examinations were performed weekly or every other week from postmenstrual age 35 weeks.

    Results: During the study, 368 infants (72.7%) developed ROP. Postmenstrual age at onset of ROP was significantly related to severity of ROP, even when controlling for gestational age (ie, the earlier the onset of ROP, the higher the risk of developing severe ROP). Site of onset of ROP was significantly related to gestational age at birth. The risk of nasal onset was almost doubled for every week of decrease in gestational age at birth. Nasal onset was associated with severe ROP, even after adjusting for gestational age at birth.

    Conclusion: This population-based study confirms results of the Cryotherapy for Retinopathy of Prematurity study and shows new correlations regarding time and site of onset of ROP, both of which are associated with disease severity.

  • 11. Ayala, Marcelo N.
    et al.
    Söderberg, Per G.
    St. Erik’s Eye Hospital, Karolinska Institutet, Stockholm, Sweden.
    Reversal of reciprocity failure for UVR-induced cataract with vitamin E2005Ingår i: Ophthalmic Research, ISSN 0030-3747, E-ISSN 1423-0259, Vol. 37, nr 3, s. 150-155Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    PURPOSE: The authors have previously described that the photochemical reciprocity law does not apply for ultraviolet radiation (UVR)-induced cataract. The aim of this study was to elucidate if failure of the reciprocity reverses with vitamin E (alpha-tocopherol) administration. METHODS: Altogether, 80 rats were divided into one group fed alpha-tocopherol and one control group. For each group, half of rats were exposed to UVR for 5 min and the remaining rats for 15 min. RESULTS: Lenses exposed to UVR for 5 min showed no difference in light scattering between alpha-tocopherol-treated and untreated groups. Lenses exposed to UVR for 15 min showed significant difference in light scattering between alpha-tocopherol-treated and untreated groups. CONCLUSIONS: Failure in exposure time-intensity reciprocity for UVR-induced cataract with exposures shorter than 30 min may be due to consumption of antioxidants in the lens.

  • 12.
    Bartuma, Katarina
    et al.
    Karolinska Inst, St Erik Eye Hosp, Dept Vitreoretinal Dis, Stockholm, Sweden..
    Pal, Niklas
    Karolinska Inst, Dept Pediat, Astrid Lindgren Childrens Hosp, S-10401 Stockholm, Sweden..
    Kosek, Sonja
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper. Uppsala Univ, Uppsala, Sweden..
    Holm, Stefan
    Karolinska Inst, Dept Pediat, Astrid Lindgren Childrens Hosp, S-10401 Stockholm, Sweden..
    All-Ericsson, Charlotta
    Karolinska Inst, St Erik Eye Hosp, Dept Vitreoretinal Dis, Stockholm, Sweden..
    A 10-year experience of outcome in chemotherapy-treated hereditary retinoblastoma2014Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 92, nr 5, s. 404-411Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose: The aim is to report the 10-year retrospective experience of systemic chemotherapy for a population-based group of patients with hereditary retinoblastoma at a national referral centre. The outcomes include control rates, treatment side-effects, adjuvant therapy, failure rate, survival, secondary cancers and visual acuity. Methods: All patients (n = 24, 46 eyes) diagnosed with retinoblastoma and treated with systemic chemotherapy at a national referral centre during 2001-2011 were included. Data were extracted from medical records. Results: The patients were followed for a mean of 60 months (range 13-144). Four-six cycles of VEC was administered to all newly diagnosed group B/C/D/E eyes with bilateral disease and 83% (38 of 46) responded to the treatment. None of the patients discontinued chemotherapy because of adverse reactions. Altogether 26% (12 of 46) of the eyes received second-line therapy (other than thermotherapy, cryotherapy and chemotherapy). The failure rate was 35% (16 of 46) and mortality rate 0%. None of the patients developed CNS manifestations (metastases or trilateral retinoblastoma). One of the patients developed a second primary tumour (osteosarcoma) 4 years following retinoblastoma diagnosis. Altogether 17% (4 of 24) patients received radiation therapy, 28% (13 of 46) of the eyes had to be enucleated, and one patient underwent bilateral enucleation. The age-correlated visual acuity was mean of 73% of expected visual acuity. Conclusion: Group A/B retinoblastomas have a distinct chemotherapy response, while group C/D/E tumours do not respond as well. The success rate was 65%; while patients have a good prognosis for life, approximately one-third of all hereditary cases received radiation therapy or underwent enucleation.

  • 13.
    Berggren, Lennart
    Uppsala universitet, Medicinska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap. Uppsala universitet, Medicinska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap. Oftalmiatrik.
    Collegium medicum och de kringresande occulisterna2003Ingår i: Svensk Medicinhistorisk Tidskrift, Vol. 7, nr 1, s. 179-189Artikel i tidskrift (Övrigt vetenskapligt)
  • 14.
    Berggren, Lennart
    Uppsala universitet, Medicinska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap. Uppsala universitet, Medicinska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap. Oftalmiatrik.
    En berättelse om en läkemedelsskandal2005Övrigt (Övrigt vetenskapligt)
  • 15.
    Berggren, Lennart
    Uppsala universitet, Medicinska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap. Uppsala universitet, Medicinska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap. Oftalmiatrik.
    Hur ögat uppfattar rörelse - ett favoritämne för både konstnär och neurofysiolog2004Ingår i: Läkartidningen, Vol. 101, nr 30-31, s. 2430-2432Artikel i tidskrift (Övrigt vetenskapligt)
  • 16.
    Bjärnhall, Gunilla
    Uppsala universitet, Medicinska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Analysis of Angiographies in Human Healthy Eyes and in Open-angle Glaucoma: Retinal Mean Transit Time and Optic Nerve Head Circulation2008Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Purpose of the studies was to develop a more robust technique to determine retinal mean transit time in healthy and in glaucoma eyes and to evaluate the circulation of the optic nerve head in glaucoma patients.

    The retinal mean transit time impulse-response method was evaluated in human healthy eyes and normal values and reproducibility were tested.

    Fluorescein and indocyanine green angiographies were recorded and the pictures were analyzed to obtain retinal mean transit time and to evaluate the proportion of low-fluorescent pixels of the optic nerve head in the glaucoma patients. Visual field defects were correlated to loss of neuroretinal rim area.

    A disturbed circulation was observed in the glaucoma patients, whether primary or secondary to loss of nerve fibre tissue can not be determined from these studies.

    Delarbeten
    1. Analysis of mean retinal transit time from fluorescein angiography in human eyes: normal values and reproducibilty.
    Öppna denna publikation i ny flik eller fönster >>Analysis of mean retinal transit time from fluorescein angiography in human eyes: normal values and reproducibilty.
    Visa övriga...
    2002 Ingår i: Acta Ophthalmologica Scand, ISSN 1395-3907, Vol. 80, nr 6, s. 652-655Artikel i tidskrift (Refereegranskat) Published
    Identifikatorer
    urn:nbn:se:uu:diva-97807 (URN)
    Tillgänglig från: 2008-11-20 Skapad: 2008-11-20Bibliografiskt granskad
    2. Retinal mean transit time in patients with primary open-angle glaucoma and normal-tension glaucoma
    Öppna denna publikation i ny flik eller fönster >>Retinal mean transit time in patients with primary open-angle glaucoma and normal-tension glaucoma
    Visa övriga...
    2007 (Engelska)Ingår i: Acta Ophthalmologica Scandinavica, ISSN 1395-3907, E-ISSN 1600-0420, Vol. 85, nr 1, s. 67-72Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Purpose: To compare the mean transit time (MTT) of retinal circulation in eyes with primary open-angle glaucoma (POAG) and eyes with normal-tension glaucoma (NTG) and examine the possible relationship between MTT and visual field damage, expressed as mean deviation (MD).

    Methods: Video fluorescein angiography was performed in 40 patients with POAG or NTG. Dye curves for fluorescein passing through the retinal arteries and veins were used to calculate MTT in each patient with a computer-assisted technique based on an impulse-response analysis (MTTir).

    Results: We were able to analyse MTTir in all 40 angiograms. Mean (SD) MTTir was 5.0 (1.5) seconds in eyes with POAG and 4.7 (1.4) seconds in eyes with NTG. The difference was not statistically significant. There was a weak but significant correlation between the MD and MTTir (MTTir = 4.12–0.08*MD; r = −0.49, p = 0.0013).

    Conslusions: The results demonstrate that loss of neuronal tissue in glaucoma is combined with an effect on the retinal circulation and that the effect is similar in eyes with NTG and eyes with POAG.

    Nyckelord
    normal-tension glaucoma, primary open-angle glaucoma, retinal blood flow, mean transit time, visual field defects, mean deviation
    Nationell ämneskategori
    Medicin och hälsovetenskap
    Identifikatorer
    urn:nbn:se:uu:diva-97808 (URN)10.1111/j.1600-0420.2006.00780.x (DOI)000243634100012 ()17244213 (PubMedID)
    Tillgänglig från: 2008-11-20 Skapad: 2008-11-20 Senast uppdaterad: 2017-12-14Bibliografiskt granskad
    3. The effect of elevated intraocular pressure on the retinal circulation in open-angle glaucoma and ocular hypertension.: A study on retinal mean transit time
    Öppna denna publikation i ny flik eller fönster >>The effect of elevated intraocular pressure on the retinal circulation in open-angle glaucoma and ocular hypertension.: A study on retinal mean transit time
    Visa övriga...
    Manuskript (Övrigt vetenskapligt)
    Identifikatorer
    urn:nbn:se:uu:diva-97809 (URN)
    Tillgänglig från: 2008-11-20 Skapad: 2008-11-20 Senast uppdaterad: 2010-01-13Bibliografiskt granskad
    4. The optic nerve head in open-angle glaucomaand ocular hypertension.: A study with fluorescein and ICG angiography
    Öppna denna publikation i ny flik eller fönster >>The optic nerve head in open-angle glaucomaand ocular hypertension.: A study with fluorescein and ICG angiography
    Visa övriga...
    Manuskript (Övrigt vetenskapligt)
    Identifikatorer
    urn:nbn:se:uu:diva-97810 (URN)
    Tillgänglig från: 2008-11-20 Skapad: 2008-11-20 Senast uppdaterad: 2010-01-13Bibliografiskt granskad
    Ladda ner fulltext (pdf)
    FULLTEXT01
  • 17.
    Blixt, Maria
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Medicinsk utvecklingsbiologi.
    Hallböök, Finn
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Medicinsk utvecklingsbiologi.
    Lineage tracing of horizontal and photoreceptor cells in the embryonic chicken retina2016Ingår i: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 57, nr 12Artikel i tidskrift (Refereegranskat)
  • 18.
    Botling Taube, Amelie
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Molecular and epidemiological studies on eyes with pseudoexfoliation syndrome2015Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Pseudoexfoliation (PEX) syndrome is an age-related condition characterized by the production and accumulation of extracellular fibrillary material in the anterior segment of the eye. PEX predisposes for several pathological conditions, such as glaucoma and complications during and after cataract surgery. The pathogenesis of PEX is not yet fully understood. It is multifactorial with genetics and ageing as contributing factors.

    We aimed to study the proteome in aqueous humor (AH) in PEX in order to increase the knowledge about its pathophysiology. Therefore, we developed sampling techniques and evaluated separation methods necessary for analyzing small sample volumes. Other objectives were to study the lens capsule in eyes with PEX regarding small molecules, and to investigate the association between PEX and cataract surgery in a population-based 30-year follow-up study.

    Samples of AH from eyes with PEX and control eyes were collected during cataract surgery. In pooled, and individual samples, various liquid based separation techniques and high resolution mass spectrometry were utilized. For quantitation, various methods for labeling, and label free techniques were applied. Lens capsules were collected from some of the patients, and analysed by imaging mass spectrometry. A cohort of 1,471 elderly individuals underwent a comprehensive ophthalmological examination at baseline. Medical information was obtained by questionnaires, and from medical records. Incident cases of cataract surgery were identified by review of medical records.

    In the initial study, several techniques were explored for protein detection, and a number of proteins were identified as differentially expressed. In the individually labelled samples, changes in the proteome were observed. Eyes with PEX contained higher levels of proteins involved in inflammation, oxidative stress, and coagulation, suggesting that these mechanisms are involved in the pathogenesis in PEX. The levels of β/γ-crystallins were significantly increased in PEX, which is a novel finding. In the lens capsules from individuals with PEX, changes in the lipid composition was observed with time-of-flight secondary ion mass spectrometry. These changes remain to be elucidated. By multivariate analysis, lens opacities were the first, and PEX the second most important predictor for cataract surgery, the later accounting for a 2.38-fold increased risk for cataract surgery.

    Delarbeten
    1. Protein content in aqueous humor from patients with pseudoexfoliation (PEX) investigated by capillary-LC MALDI-TOF/TOF MS
    Öppna denna publikation i ny flik eller fönster >>Protein content in aqueous humor from patients with pseudoexfoliation (PEX) investigated by capillary-LC MALDI-TOF/TOF MS
    Visa övriga...
    2009 (Engelska)Ingår i: PROTEOMICS - Clinical Applications, ISSN 1862-8346, E-ISSN 1862-8354, Vol. 3, nr 3, s. 299-306Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Analysis of proteins in human body fluids is challenging since the composition of the sample often is rather complex. Here we present a method for analysis of proteins in aqueous humor from two groups of cataract patients, with and without pseudoexfoliation (PEX). Aqueous humor is an extracellular fluid contained in the anterior chamber of the eye between the cornea and iris. The limited volume of sample requires sophisticated analysis techniques. Our method is based on a total tryptic digestion of the sample followed by capillary LC-MALDI MS and MS/MS analysis of the peptides. The method is rapid, efficient and suitable as a complement or alternative to more commonly used methods based on gel electrophoretic experiments. With this method we found and unambiguously identified 30 nonredundant proteins. Proteins found include general transport proteins such as albumin and apolipoprotein A1 but also specific proteins involved in immune response, such as   complement factors. Cystatin C, clusterin, and crystallins were also found. Although the number of proteins was roughly the same in both groups there was a significant difference in their identities. These findings may give some new insights into the pathophysiology of the PEX syndrome.

    Nyckelord
    Aqueous humor, Capillary liquid chromatography, Human body fluids, MALDI-TOF MS/MS, Pseudoexfoliation syndrome
    Nationell ämneskategori
    Medicin och hälsovetenskap
    Identifikatorer
    urn:nbn:se:uu:diva-97814 (URN)10.1002/prca.200780077 (DOI)000264794700002 ()
    Tillgänglig från: 2008-11-20 Skapad: 2008-11-20 Senast uppdaterad: 2017-12-14Bibliografiskt granskad
    2. Proteins in aqueous humor from cataract patients with and without pseudoexfoliation syndrome
    Öppna denna publikation i ny flik eller fönster >>Proteins in aqueous humor from cataract patients with and without pseudoexfoliation syndrome
    Visa övriga...
    2012 (Engelska)Ingår i: European journal of mass spectrometry, ISSN 1469-0667, E-ISSN 1751-6838, Vol. 18, nr 6, s. 531-541Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    The aim of this study was to investigate the protein content in aqueous humor in eyes with and without pseudoexfoliations (PEX) and to evaluate the quantitative proteomics method, isobaric tagging for relative and absolute protein quantification (iTRAQ), in combination with two separation methods followed by matrix-assisted Laser desorption/ionization (MALDI) mass spectrometry and tandem mass spectrometry (MS/MS). During cataract surgery, samples of aqueous humor were collected from 20 eyes with PEX and from 18 control eyes. The relative concentrations of proteins in the pooled samples of ten PEX eyes and eight controls were evaluated after trypsin digestion and labeling of the peptides with (iTRAQ) reagent. Two separation methods, Liquid chromatography (LC) and capillary electrophoresis (CE) were used, followed by MALDI mass spectrometry and MS/MS. Furthermore, 1D gel electrophoresis was performed on the remaining ten pooled PEX samples and ten control samples. The gel material was separated by nano-liquid chromatography (nano-LC) followed by Linear-ion-trap quadrupole Fourier transformation ion cyclotron resonance (FT-ICR). Fifty four proteins were identified in the LC runs and 24 with CE. The relative concentrations of beta-crystallines B2 and S were raised and those of angiotensinogen and osteopontin lowered in the PEX sample compared to the control. The trends regarding beta-crystallines B2, angiotensinogen and osteopontin were confirmed by the 1D gel electrophoresis.

    Nyckelord
    pseudoexfoliations (PEX), isobaric tagging, protein quantification, proteomics, aqueous humour, osteopontin, angiotensinogen
    Nationell ämneskategori
    Medicin och hälsovetenskap
    Identifikatorer
    urn:nbn:se:uu:diva-197670 (URN)10.1255/ejms.1208 (DOI)000315745600007 ()
    Tillgänglig från: 2013-04-02 Skapad: 2013-04-02 Senast uppdaterad: 2017-12-06Bibliografiskt granskad
    3. Proteomic Analysis of the Aqueous Humor in Eyes with Pseudoexfoliation Syndrome
    Öppna denna publikation i ny flik eller fönster >>Proteomic Analysis of the Aqueous Humor in Eyes with Pseudoexfoliation Syndrome
    (Engelska)Artikel i tidskrift (Refereegranskat) Submitted
    Nyckelord
    Aqueous humor; Pseudoexfoliation syndrome; Quantitative proteomics; Dimethyl labelling; Mass spectrometry
    Nationell ämneskategori
    Oftalmologi Analytisk kemi
    Forskningsämne
    Oftalmiatrik
    Identifikatorer
    urn:nbn:se:uu:diva-260949 (URN)
    Tillgänglig från: 2015-08-26 Skapad: 2015-08-26 Senast uppdaterad: 2015-10-01
    4. Imaging of Human Lens Capsules with Pseudoexfoliation Syndrome by Time of Flight Secondary Ion Mass Spectrometry (TOF-SIMS)
    Öppna denna publikation i ny flik eller fönster >>Imaging of Human Lens Capsules with Pseudoexfoliation Syndrome by Time of Flight Secondary Ion Mass Spectrometry (TOF-SIMS)
    (Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
    Nyckelord
    PEX, cataract, lens capsule, time of flight secondary mass spectrometry (TOF-SIMS), imaging mass spectrometry (IMS), sphingolipids
    Nationell ämneskategori
    Oftalmologi
    Forskningsämne
    Oftalmiatrik; Kemi med inriktning mot analytisk kemi
    Identifikatorer
    urn:nbn:se:uu:diva-260946 (URN)
    Forskningsfinansiär
    Vetenskapsrådet, 621-2011-4423
    Tillgänglig från: 2015-08-26 Skapad: 2015-08-26 Senast uppdaterad: 2015-10-01
    5. Pseudoexfoliation and cataract surgery: a population-based 30-year follow-up study
    Öppna denna publikation i ny flik eller fönster >>Pseudoexfoliation and cataract surgery: a population-based 30-year follow-up study
    2015 (Engelska)Ingår i: Acta Ophthalmologica Scandinavica, ISSN 1395-3907, E-ISSN 1600-0420, Vol. 93, nr 8, s. 774-777Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    PURPOSE:

    To study the relationship between pseudoexfoliation (PEX) and cataract surgery in a population in which PEX is a common finding.

    METHODS:

    Survival analyses were performed in a cohort of 760 residents 65-74 years of age, examined in a population survey in the municipality of Tierp, Sweden, during 1984-1986. To expand the cohort, participants in other studies in Tierp were enrolled. Additionally, subjects were recruited by means of glaucoma case records established at the Eye Department in Tierp in 1978-2007. In total, the cohort comprised 1471 individuals, representing more than 15 900 person-years of risk. Information on cataract surgery was obtained from the glaucoma case records and from medical records.

    RESULTS:

    By the end of the study in August 2014, 564 subjects had undergone cataract surgery. Of these cases, 224 were affected by PEX at baseline. In multivariate analyses, PEX was the second most important predictor for cataract surgery after lens opacities, accounting for a 2.38-fold (95% confidence interval 2.01-2.84) increased risk.

    CONCLUSION:

    The results strongly suggest that PEX is a predictor for cataract surgery in the population under study.

    Ort, förlag, år, upplaga, sidor
    John Wiley & Sons, 2015
    Nyckelord
    cataract, cataract surgery, epidemiology, exfoliation syndrome, pseudoexfoliation, risk factor
    Nationell ämneskategori
    Oftalmologi
    Forskningsämne
    Oftalmiatrik
    Identifikatorer
    urn:nbn:se:uu:diva-260948 (URN)10.1111/aos12789 (DOI)000367654500034 ()
    Tillgänglig från: 2015-08-26 Skapad: 2015-08-26 Senast uppdaterad: 2017-12-04Bibliografiskt granskad
    Ladda ner fulltext (pdf)
    fulltext
    Ladda ner (jpg)
    presentationsbild
  • 19.
    Botling Taube, Amelie
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
    Konzer, Anne
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
    Alm, Albert
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Bergquist, Jonas
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
    Proteomic Analysis of the Aqueous Humor in Eyes with Pseudoexfoliation SyndromeArtikel i tidskrift (Refereegranskat)
  • 20.
    Botling Taube, Amelie
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap. Karolinska Inst, Dept Clin Neurosci, St Erik Eye Hosp, Stockholm, Sweden.
    Konzer, Anne
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
    Alm, Albert
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Bergquist, Jonas
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
    Proteomic analysis of the aqueous humour in eyes with pseudoexfoliation syndrome2019Ingår i: British Journal of Ophthalmology, ISSN 0007-1161, E-ISSN 1468-2079, Vol. 103, nr 8, s. 1190-1194Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background/aims Pseudoexfoliation syndrome (PEX) is characterised by the production and accumulation of extracellular fibrillar material in the anterior segment of the eye. The pathogenesis of PEX is multifactorial with genetic factors and ageing as contributing factors. Previously, an increased concentration of beta-crystalline B2 (CRYBB2) was observed in the aqueous humour (AH) in eyes with PEX in a pooled material. Here, the protein content was examined on individual basis. Methods During cataract surgery, AH was sampled from patients with and without PEX, 10 eyes in each group. The proteins were digested and labelled with isotopomeric dimethyl labels, separated with high-pressure liquid chromatography and analysed in an Orbitrap mass analyzer. Results The concentration of complement factor 3, kininogen-1, antithrombin III and vitamin D-binding protein was increased in all eyes with PEX. Retinol-binding protein 3, glutathione peroxidase, calsyntenin-1 and carboxypeptidase E were decreased in eyes with PEX. Beta-crystalline B1 and CRYBB2 and gamma-crystalline D were up to eightfold upregulated in 4 of 10 in eyes with PEX. Conclusion The results indicate that oxidative stress and inflammation are contributing factors in the formation of PEX. Knowledge about the proteome in PEX is relevant for understanding this condition.

    Ladda ner fulltext (pdf)
    fulltext
  • 21.
    Botling Taube, Amelie
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
    Mi, Jia
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
    Alm, Albert
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Bergquist, Jonas
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
    Imaging of Human Lens Capsules with Pseudoexfoliation Syndrome by Time of Flight Secondary Ion Mass Spectrometry (TOF-SIMS)Manuskript (preprint) (Övrigt vetenskapligt)
  • 22. Breitbart, Eberhardt
    et al.
    Cesarini, Jean-Pierre
    de Gruijl, Frank
    Hietanen, Marja
    Mariutti, G
    Mc Kinley, Alister
    Okuno,
    Roy, C
    Schulmeister, Karl
    Sliney, David
    Söderberg, Per
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Stuck, Bruce
    Swerdlow, Anthony
    Van Deventer, E
    Zeeb, H
    Protecting workers from ultraviolet radiation2007Bok (Övrigt vetenskapligt)
  • 23.
    Bucht, Curry
    et al.
    Inst. för Klinisk Neurovetenskap, KI.
    Manneberg, Göran
    Biomedicinsk fysik och röntgenfysik, KTH.
    Recording the diffraction pattern reflected from corneal endothelium.2007Ingår i: Ophthalmic Technologies XVII / [ed] Manns F, Söderberg PG, Ho Arthur, Bellinghamn, WA, USA: SPIE , 2007, s. 10:1-10:8Konferensbidrag (Refereegranskat)
  • 24. Bucht, Curry
    et al.
    Söderberg, Per G.
    St. Erik's Eye Hospital (Sweden) and Mälarsjukhuset Eskilstuna (Sweden) and Univ. of Miami.
    Manneberg, Göran
    A model for corneal endothelial morphometry by diffraction2006Ingår i: Ophthalmic Technologies XVI: Proceedings of SPIE / [ed] Fabrice Manns; Per G. Söderberg; Arthur Ho, San José, CA: SPIE , 2006, Vol. 6138, s. 6138-O-1-6138-O-8Konferensbidrag (Refereegranskat)
  • 25. Bucht, Curry
    et al.
    Söderberg, Per G.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Manneberg, Göran
    Fully automated corneal endothelial morphometry of images captured by clinical specular microscopy2010Ingår i: Ophthalmic Technologies XX / [ed] Fabrice Manns, Per G. Söderberg, Arthur Ho, San Francisco, CA: SPIE , 2010, s. 1E-1-1E-8Konferensbidrag (Refereegranskat)
  • 26. Bucht, Curry
    et al.
    Söderberg, Per G.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Manneberg, Göran
    Fully automated corneal endothelial morphometry of images captured by clinical specular microscopy2009Ingår i: Ophthalmic Technologies XIX / [ed] Fabrice Manns; Per G. Söderberg; Arthur Ho, San José, CA: SPIE , 2009, Vol. 7163, s. 15:1-15:8Konferensbidrag (Refereegranskat)
  • 27. Bucht, Curry
    et al.
    Söderberg, Per G.
    St. Erik's Eye Hospital (Sweden) and Univ. of Miami.
    Manneberg, Göran
    The impact of horizontal offset of the cornea during corneal specular microscopy2008Ingår i: Ophthalmic Technologies XVIII: Proceedings of SPIE / [ed] Fabrice Manns; Per G. Söderberg; Arthur Ho; Bruce E. Stuck; Michael Belkin, San José, CA: SPIE , 2008, Vol. 6426, s. 13-1-13-9Konferensbidrag (Refereegranskat)
  • 28. Bucht, Curry
    et al.
    Söderberg, Per
    St Erik's Eye Hospital (Sweden) and Univ. of Miami.
    Manneberg, Göran
    Recording the dffraction pattern reflected from corneal endothelium2007Ingår i:  Ophthalmic Technologies XVII / [ed] Fabrice Manns, Per G. Söderberg, Arthur Ho, Bruce E. Stuck, Michael Belkin, San José, CA: SPIE , 2007, Vol. 6426, s. 10:1-10:8Konferensbidrag (Refereegranskat)
  • 29.
    Bucht, Curry
    et al.
    S:t Eriks Ögonsjukhus, Inst. f. Neurovetenskap, KI.
    Söderberg, Per
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Manneberg, Göran
    Biomedicinsk fysik och röntgenfysik, Inst. för tillämpad fysik.
    Simulation of specular microscopy images of corneal endothelium, a tool for control of measurement errors2011Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 89, nr 3, s. e242-e250Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose: We aimed at developing simulation software capable of producing images of corneal endothelium close to identical to images captured by clinical specular microscopy with defined morphometrical characteristics. It was further planned to demonstrate the usefulness of the simulator by analysing measurement errors associated with a trained operator using a commercially available semi-automatic algorithm for analysis of simulated images.

    Methods: Software was developed that allows creation of unique images of the corneal endothelium expressing morphology close to identical with that seen in images of corneal specular microscopy. Several hundred unique images of the corneal endothelium were generated with randomization, spanning a physiological range of endothelial cell density. As an example of the usefulness of the simulator for analysis of measurement errors in corneal specular microscopy, a total of 12 of all the images generated were randomly selected such that the endothelial cell density expressed was evenly distributed over the physiological range of endothelial cell density. The images were transferred to a personal computer. The imagenet-640 software was used to analyse endothelial cell size variation, percentage of hexagonal endothelial cells, and endothelial cell density.

    Results: The simulator developed allows randomized generation of corneal specular microscopy images with a preset expected average and variation of cell structure. Calculated morphometric information of each cell is stored in the simulator. The image quality can secondarily be varied with a toolbox of filters to approximate a large spectrum of clinically captured images. As an example of the use of the simulator, measurement errors associated with one trained operator using the imagenet-640 software, and focusing on endothelial cell density, were examined. The functional dependence between morphometric information estimated with the imagenet-640 software algorithm and real morphometric information as provided by the simulator was analysed with regression. It was demonstrated that that the estimations of endothelial cell size variation was associated with a scaling error and that the random error was strongly dependent on the operator.

    Conclusion: The newly developed simulator for randomized generation of morphometrically defined corneal specular microscopy images for the first time makes it possible to estimate a spatial scaling error of an available semi-automatic algorithm and to determine the random measurement error of important morphometric estimates in a defined reference sample of images. It is anticipated that the simulator will be a valuable tool for the generation of a large set of morphometrically well-characterized corneal specular microscopy images that can be used for calibration among research centres, for minimization of random errors and for measurement of quality control. Simulated images will be useful for the development of fully automatic analysis of corneal endothelial cell morphometry.

  • 30.
    Carizzo, Gabriel
    et al.
    KTH, Dept. of Biomedical Engineering and Health Systems.
    Yu, Zhaohua
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Wang, Chunliang
    KTH, Dept. of Biomedical Engineering and Health Systems.
    Sandberg Melin, Camilla
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik. Center for Research and Development, Region Gävleborg.
    Kisonaite, Konstancia
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Söderberg, Per
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Fully automatic estimation of the angular distribution of the waist of the nerve fiber layer in the optic nerve head.2020Ingår i: SPIE, BIOS, Ophthalmic Technologies XXX / [ed] Manns F, Ho A, Söderberg PG, Bellingham: SPIE , 2020, Vol. 11218Konferensbidrag (Refereegranskat)
  • 31. Castellini, Meryl
    et al.
    Wolf, Louise V.
    Chauhan, Bharesh K.
    Galileo, Deni S.
    Kilimann, Manfred W.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för cell- och molekylärbiologi.
    Cvekl, A.
    Duncan, Melina K.
    Palm is expressed in both developing and adult mouse lens and retina2005Ingår i: BMC Ophthalmology, ISSN 1471-2415, E-ISSN 1471-2415, Vol. 5, s. 14-Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background

    Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Earlier studies have shown brain preferred Palm expression, although this protein is a major water insoluble protein in chicken lens fiber cells and the Palm gene may be regulated by Pax6.

    Methods

    The expression profile of Palm protein in the embryonic, newborn and adult mouse eye as well as dissociated retinal neurons was determined by confocal immunofluorescence. The relative mRNA levels of Palm, Palmdelphin (PalmD) and paralemmin2 (Palm2) in the lens and retina were determined by real time rt-PCR.

    Results

    In the lens, Palm is already expressed at 9.5 dpc in the lens placode, and this expression is maintained in the lens vesicle throughout the formation of the adult lens. Palm is largely absent from the optic vesicle but is detectable at 10.5 dpc in the optic cup. In the developing retina, Palm expression transiently upregulates during the formation of optic nerve as well as in the formation of both the inner and outer plexiform layers. In short term dissociated chick retinal cultures, Palm protein is easily detectable, but the levels appear to reduce sharply as the cultures age. Palm mRNA was found at much higher levels relative to Palm2 or PalmD in both the retina and lens.

    Conclusion

    Palm is the major paralemmin family member expressed in the retina and lens and its expression in the retina transiently upregulates during active neurite outgrowth. The expression pattern of Palm in the eye is consistent with it being a Pax6 responsive gene. Since Palm is known to be able to drive membrane formation in brain neurons, it is possible that this molecule is crucial for the increase in membrane formation during lens fiber cell differentiation.

  • 32.
    Colafella, Claudio
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi.
    Figure reuse in Exp Eye Res. (2018); 167: 152–1622019Ingår i: Experimental Eye Research, ISSN 0014-4835, E-ISSN 1096-0007, Vol. 181, s. 374-374Artikel i tidskrift (Övrigt vetenskapligt)
  • 33.
    Dalin, Frida
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Dermatologi och venereologi. Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Stockholm, Sweden..
    Adamus, Grazyna
    Oregon Hlth & Sci Univ, Casey Eye Inst, Ocular Immunol Lab, Portland, OR 97201 USA..
    Yang, Sufang
    Oregon Hlth & Sci Univ, Casey Eye Inst, Ocular Immunol Lab, Portland, OR 97201 USA..
    Landgren, Eva
    Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Stockholm, Sweden..
    Palle, Josefine
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kvinnors och barns hälsa, Pediatrik.
    Hallgren, Åsa
    Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Stockholm, Sweden..
    Frost, Britt-Marie
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kvinnors och barns hälsa, Pediatrik.
    Hugosson, Therese
    Lund Univ, Dept Clin Sci, Ophthalmol, Lund, Sweden..
    Landegren, Nils
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Autoimmunitet. Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Stockholm, Sweden..
    Eriksson, Daniel
    Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Stockholm, Sweden..
    Andreasson, Sten
    Lund Univ, Dept Clin Sci, Ophthalmol, Lund, Sweden..
    Tabbara, Khalid F.
    Ctr Eye, Riyadh, Saudi Arabia..
    Kämpe, Olle
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Autoimmunitet. Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Stockholm, Sweden..
    Alimohammadi, Mohammad
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Dermatologi och venereologi. Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Stockholm, Sweden..
    Aryl Hydrocarbon Receptor-Interacting Protein-Like 1 in Cancer-Associated Retinopathy2016Ingår i: Ophthalmology (Rochester, Minn.), ISSN 0161-6420, E-ISSN 1549-4713, Vol. 123, nr 6, s. 1401-1404Artikel i tidskrift (Övrigt vetenskapligt)
  • 34.
    Ekström, Curt
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för folkhälso- och vårdvetenskap, Socialmedicin.
    Prevalence of open-angle glaucoma in Central Sweden: The Tierp Glaucoma Survey1996Ingår i: Acta Ophthalmologica Scandinavica, ISSN 1395-3907, E-ISSN 1600-0420, Vol. 74, nr 2, s. 107-112Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    In 1984‐86, a glaucoma survey was carried out in a municipality in central Sweden, comprising 760 subjects 65–74 years of age. Since 1988, a follow‐up study of those 527 subjects who had normal and reliable visual fields has been in progress. A visual field defect was required for a diagnosis of chronic open‐angle glaucoma. By December 1991, 10 cases of capsular glaucoma and 3 cases of chronic simple glaucoma had been found. The standardized relative risk for chronic open‐angle glaucoma was 3.4 times (95% confidence interval: 1.1 ‐ 11) greater in subjects with a baseline intraocular pressure < 21 mmHg, using the highest pressure in either eye, than in those with an intraocular pressure < 21 mmHg. Pseudoexfoliation increased the standardized relative risk 9.8‐fold (95% confidence interval: 2.5 – 38). There was indication of effect modification between intraocular pressure and pseudoexfoliation. Optic disc haemorrhages were associated with a 4.8‐fold (95% confidence interval: 0.63 – 37) increased unadjusted risk. The study supports the pressure theory of optic nerve damage in glaucoma. Pseudoexfoliation was recognized as an independent risk factor for the development of chronic open‐angle glaucoma.

  • 35.
    Ekström, Curt
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Risk factors for incident open-angle glaucoma: a population-based 20-year follow-up study2012Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 90, nr 4, s. 316-321Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose: To study the effect of potential risk factors on the development of open-angle glaucoma (OAG) in a population in which pseudoexfoliation (PEX) is a common finding.

    Methods: In 1984-1986, a population-based survey of 760 people aged 65-74 years was conducted in the municipality of Tierp, Sweden. From 1988 to 2006, a follow-up study of the 530 people with normal visual fields has been in progress. To increase the cohort, 273 ophthalmic outpatients were enroled. Reliable visual fields were available for 679 people, representing 6 126 person-years at risk. A time-weighted mean intraocular pressure (IOP) for all visits was calculated.

    Results: Sixty-four subjects developed definite OAG, 29 of whom were exposed to PEX. Risk factors associated with OAG were higher age, a positive family history, increased IOP and PEX. The age-standardized rate ratio (SRR) was 14.8 times (95% confidence interval [CI] 7.92-27.8) greater in subjects with mean IOP >/=20 mmHg than in those with mean IOP /=20 mmHg.

    Conclusion: Increased IOP and PEX were serious risk factors for incident OAG. The effect of PEX was mediated by increased IOP.

  • 36.
    Ekström, Curt
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Botling Taube, Amelie
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Pseudoexfoliation and cataract surgery: a population-based 30-year follow-up study2015Ingår i: Acta Ophthalmologica Scandinavica, ISSN 1395-3907, E-ISSN 1600-0420, Vol. 93, nr 8, s. 774-777Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    PURPOSE:

    To study the relationship between pseudoexfoliation (PEX) and cataract surgery in a population in which PEX is a common finding.

    METHODS:

    Survival analyses were performed in a cohort of 760 residents 65-74 years of age, examined in a population survey in the municipality of Tierp, Sweden, during 1984-1986. To expand the cohort, participants in other studies in Tierp were enrolled. Additionally, subjects were recruited by means of glaucoma case records established at the Eye Department in Tierp in 1978-2007. In total, the cohort comprised 1471 individuals, representing more than 15 900 person-years of risk. Information on cataract surgery was obtained from the glaucoma case records and from medical records.

    RESULTS:

    By the end of the study in August 2014, 564 subjects had undergone cataract surgery. Of these cases, 224 were affected by PEX at baseline. In multivariate analyses, PEX was the second most important predictor for cataract surgery after lens opacities, accounting for a 2.38-fold (95% confidence interval 2.01-2.84) increased risk.

    CONCLUSION:

    The results strongly suggest that PEX is a predictor for cataract surgery in the population under study.

  • 37.
    Ekström, Curt
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Kilander, Lena
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för folkhälso- och vårdvetenskap, Geriatrik. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Open-angle glaucoma and Alzheimer´s disease: a population-based 30 year follow-up study2017Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 95, nr 2, s. E157-E158Artikel i tidskrift (Övrigt vetenskapligt)
  • 38.
    Ekström, Curt
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Kilander, Lena
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för folkhälso- och vårdvetenskap, Geriatrik.
    Pseudoexfoliation and Alzheimer´s disease: a population-based 30-year follow-up study2014Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 92, nr 4, s. 355-358Artikel i tidskrift (Refereegranskat)
  • 39.
    Ekström, Curt
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Wilger, Sophia
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Wanhainen, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kirurgiska vetenskaper, Kärlkirurgi.
    Pseudoexfoliation and aortic aneurysm: a long-term follow-up study2019Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 97, nr 1, s. 80-83Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    PURPOSE: To study the relationship between pseudoexfoliation (PEX) and aortic aneurysm in a population with a high prevalence of PEX.

    METHODS: Survival analyses were performed in a cohort of 735 residents aged 65-74 years, examined in a population survey in the municipality of Tierp, Sweden, 1984-1986. To expand the sample size, 1040 people were recruited by means of glaucoma case records established at the Eye Department in Tierp in 1978-2007. In this way, the cohort comprised 1775 subjects, representing more than 25 400 person-years at risk. Medical records and autopsy reports were reviewed to identify subjects diagnosed with aneurysm. Those with a follow-up time shorter than 1 year were excluded.

    RESULTS: By the end of the study in September 2017, 60 new cases of aortic aneurysm had been found. Of these cases, 23% (14 subjects) were affected by PEX at baseline, compared with 28% among subjects without aneurysm. No association between PEX and aortic aneurysm was found (hazard ratio 0.97; 95% confidence interval 0.53-1.77).

    CONCLUSION: In this population-based study, we were unable to verify a relationship between PEX and the development of aortic aneurysm.

  • 40.
    Eriksson, Urban
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Aspects of Optical Coherence Tomography (OCT) in Healthy Eyes and Eyes with Retinal Diseases2010Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Optical coherence tomography (OCT) is a technique in which cross-sectional images from intraocular tissue can be obtained. The quantitative and qualitative examinations are used for evaluating retinal diseases. Conventional OCT (Stratus) is mainly used, but the new Spectral domain (Cirrus) OCT, which has improved technology, may provide more reliable measurements.

    The aim of the study was to collect normal values of macular thickness in children and adults and to evaluate the effect of age and/or gender, to compare measurement variability in healthy eyes and eyes with age-related macular degeneration (AMD), to compare Stratus and Cirrus OCT and to study the effect of cataract surgery on macula.

    Sixty-seven healthy adults and 56 children, 30 patients with AMD, 34 patients with diabetes and cataract and 35 healthy controls were included. The quantitative maps in Stratus and Cirrus were used and manual correction of foveal location was evaluated. Qualitative OCT was compared to fluorescein angiography (FA) after cataract surgery.

    The mean values of macular thickness in Stratus OCT were 207µm in adults and 204 µm in children. The measurement variability was low. Macular thickness decreased with age in adults, but not in children. No correlation with gender was found. In eyes with wet AMD, there were small differences in measurement variability comparing Stratus and Cirrus OCT. After manual correction in Cirrus OCT, the coefficients of repeatability were improved to values close to the repeatability in normal eyes. Two thirds of the diabetic and half of the control eyes showed leakage on FA after cataract surgery. Qualitative OCT corresponded poorly to FA in diabetic eyes. A thicker macula, assessed with OCT, was often observed without any obvious effect on visual acuity.  OCT was as good as FA in revealing clinically relevant changes in macula after surgery, and was the technique recommended for follow-up.

    Delarbeten
    1. Repeatability in and interchangeability between the macular and the fast macular thickness map protocols: a study on normal eyes with Stratus optical coherence tomography
    Öppna denna publikation i ny flik eller fönster >>Repeatability in and interchangeability between the macular and the fast macular thickness map protocols: a study on normal eyes with Stratus optical coherence tomography
    2009 (Engelska)Ingår i: Acta ophthalmologica, ISSN 1755-3768, Vol. 87, nr 7, s. 725-730Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Purpose: To collect a normal material and to compare the macular and the fast macular thickness map protocols regarding normal values and repeatability. Methods: Sixty-seven individuals underwent three repeated scans with the macular thickness protocol; 45 of them also had three scans with the fast thickness protocol in Stratus optical coherence tomography (OCT). The maps were divided into nine ETDRS fields, where thickness values were presented. The repeatability was calculated as intraclass correlation coefficient (ICC), coefficient of variance (CV) and coefficient of repeatability (CR). For comparison between the two protocols, limits of agreement were determined according to Bland-Altman. Results: Normal values for the two protocols were very close. Repeatability was high. ICC for all areas was 0.92-0.98. CV was less than 1% and CR was 6-8 mum for both protocols, with the exception of the fovea in the fast protocol (where CV was 1.44% and CR 12.4 mum). Limits of agreement between the two protocols were less than 10 mum as a rule. Conclusion: Normal values for the protocols are equal and they both have excellent repeatability. The fast macular map is a good alternative with the possible exception of the fovea, where variation is twice that of the macular thickness map.

    Nyckelord
    macular thickness maps, normal values, optical coherence tomography, repeatability
    Nationell ämneskategori
    Medicin och hälsovetenskap
    Identifikatorer
    urn:nbn:se:uu:diva-104911 (URN)10.1111/j.1755-3768.2008.01345.x (DOI)000270999300006 ()18937816 (PubMedID)
    Tillgänglig från: 2009-05-30 Skapad: 2009-05-30 Senast uppdaterad: 2010-09-23Bibliografiskt granskad
    2. A population-based study of macular thickness in full-term children assessed with Stratus OCT: normative data and repeatability.
    Öppna denna publikation i ny flik eller fönster >>A population-based study of macular thickness in full-term children assessed with Stratus OCT: normative data and repeatability.
    2009 (Engelska)Ingår i: Acta ophthalmologica, ISSN 1755-3768, Vol. 87, nr 7, s. 741-745Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Purpose: We aimed to determine normal macular thickness values, assessed with optical coherence tomography (OCT), in a population of full-term children of normal birthweight. Methods: A total of 56 children, aged 5-16 years, randomly chosen from the population register, were examined with Stratus OCT. Only children with visual acuity < 0.2 logMAR, spherical equivalent of - 3 to + 3 D and astigmatism < 2 D were included. The fast macular map protocol was used and three examinations were performed in each eye. One eye was then randomized for further analyses. Mean values for the nine ETDRS areas, foveal minimum thickness and macular volume were calculated for 55 eyes. Coefficients of variance and intraclass correlations were calculated for each area. Results: All children co-operated well and no child was excluded for lack of concentration. Mean +/- standard deviation central macular thickness was 204 +/- 19 mum. Mean total macular volume was 7.11 +/- 0.35 mm(3). No correlations were found between age, gender and macular thickness. Coefficients of variance were < 2% and intraclass correlations were > 0.9 in all areas, except the foveal minimum. Conclusions: Normal values for macular thickness in healthy full-term children were reported. As the Stratus OCT provides normal values only for adults, these data are a better alternative for comparison with children with retinal abnormalities. We concluded that OCT is suitable for examining the retina in children aged 5-16 years and has the same high level of repeatability as in adults.

    Nyckelord
    children, full-term, macular thickness, optical coherence tomography (OCT), repeatability
    Nationell ämneskategori
    Medicin och hälsovetenskap
    Identifikatorer
    urn:nbn:se:uu:diva-104400 (URN)10.1111/j.1755-3768.2008.01357.x (DOI)000270999300009 ()18811639 (PubMedID)
    Tillgänglig från: 2009-05-30 Skapad: 2009-05-28 Senast uppdaterad: 2010-10-04Bibliografiskt granskad
    3. Macular thickness decreases with age in normal eyes: A study on the macular thickness map protocol in the Stratus OCT
    Öppna denna publikation i ny flik eller fönster >>Macular thickness decreases with age in normal eyes: A study on the macular thickness map protocol in the Stratus OCT
    2008 (Engelska)Ingår i: British Journal of Ophthalmology, ISSN 0007-1161, E-ISSN 1468-2079, Vol. 93, nr 11, s. 1148-1452Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    BACKGROUND/AIM: Retinal and retinal nerve fibre layer (RNFL) thinning with age have been described in histological studies. In vivo techniques like optical coherence tomography (OCT), has shown thinning of optic nerve RNFL and the retina in specific areas. One would expect thinning of the total macula, but so far, a correlation with the quantitative OCT macular map tool and age has not been found. METHODS: Sixty-seven healthy individuals underwent three repeated scans in both eyes with the macular thickness map protocol in the Stratus OCT. That protocol divides the macula area in nine ETDRS fields.The RNFL was measured in one specific location close to the optic disc. Correlations between retinal, RNFL thickness, macular volume and age were determined. RESULTS: We found a statistically significant negative relationship between retinal thickness and age for all ETDRS areas, total macular volume and RNFL thickness. Retinal thickness decreased with 0,26-0,46 microm, the macula volume 0,01 mm(3) and RNFL with 0,09 microm per year. CONCLUSION: Retinal thickness within the area covered by the macular map significantly decreases with age. In the area examined in the papillomacular bundle, 20% of the retinal thinning is due to the RNFL and 80% due to thinning of other layers of the retina.

    Nationell ämneskategori
    Medicin och hälsovetenskap
    Identifikatorer
    urn:nbn:se:uu:diva-104910 (URN)10.1136/bjo.2007.131094 (DOI)000271091900009 ()19019921 (PubMedID)
    Tillgänglig från: 2009-05-30 Skapad: 2009-05-30 Senast uppdaterad: 2017-12-13Bibliografiskt granskad
    4. Is quantitative spectral-domain superior to time-domain optical coherence tomography (OCT) in eyes with age-related macular degeneration?
    Öppna denna publikation i ny flik eller fönster >>Is quantitative spectral-domain superior to time-domain optical coherence tomography (OCT) in eyes with age-related macular degeneration?
    2012 (Engelska)Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 90, nr 7, s. 620-627Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Purpose: The aim of this study was to determine the variability of macular map measurements, for two generations of optical coherence tomography (OCT) instruments, in eyes with wet age related macular degeneration (AMD) and low visual acuity. Methods: Patients were examined with Stratus OCT and Cirrus HD-OCT. The macular thickness was assessed with the 'macular thickness map scan' and 'fast protocol' in Stratus and with the 512 × 128 and 200 × 200 cube protocols in Cirrus OCT. Two measurements were taken one directly after the other, at the first visit to analyse repeatability. Approximately 1 week later, a third measurement was taken to analyse reproducibility. In Cirrus OCT, a manual correction of foveal location was also performed. Repeatability and reproducibility were calculated as a coefficient of variance (CoV) and a coefficient of repeatability/reproducibility. Results: Repeatability for central macular thickness (expressed as CoV) was about three per cent for all protocols, and the coefficient of repeatability between 34 and 54 μm. Reproducibility (also expressed as CoV) was between four to seven per cent and coefficient of repeatability between 64 and 89 μm. After manual adjustment of foveal location in Cirrus OCT, the coefficient of repeatability improved to 12-18 μm, and the coefficient of reproducibility to 44-47 μm. Conclusions: In eyes affected by wet AMD, there were small differences in repeatability and reproducibility when comparing quantitative maps in Stratus and Cirrus OCT. However, when the software for manual correction of foveal position in Cirrus OCT was used, the variability decreased markedly, and the repeatability was close to what had been reported in normal eyes, demonstrating a significant, potential advantage of spectral-domain over time-domain OCT.

    Nyckelord
    age-related macular degeneration, quantitative optical coherence tomography, repeatability, reproducibility
    Nationell ämneskategori
    Oftalmologi
    Forskningsämne
    Oftalmiatrik
    Identifikatorer
    urn:nbn:se:uu:diva-184945 (URN)10.1111/j.1755-3768.2011.02112.x (DOI)000310548500017 ()
    Tillgänglig från: 2012-11-19 Skapad: 2012-11-15 Senast uppdaterad: 2017-12-07Bibliografiskt granskad
    5. Macular edema and visual outcome following cataract surgery in patients with diabetic retinopathy and controls
    Öppna denna publikation i ny flik eller fönster >>Macular edema and visual outcome following cataract surgery in patients with diabetic retinopathy and controls
    Visa övriga...
    2011 (Engelska)Ingår i: Graefe's Archives for Clinical and Experimental Ophthalmology, ISSN 0721-832X, E-ISSN 1435-702X, Vol. 249, nr 3, s. 349-359Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Cystoid macular edema (CME) is a well-known complication after cataract surgery, and diabetic retinopathy is reported to be an important risk factor for impaired visual recovery. In this prospective study, we compared visual outcome 6 months after surgery in eyes with moderate retinopathy and no previous ME with a control group, and observed the incidence of ME seen on fluorescein angiography (FA) and optical coherence tomography (OCT).

    Thirty-four patients with type-2 diabetes and 35 controls were enrolled. Best-corrected visual acuity (VA) letters ETDRS was measured pre-op, at day 7, week 6 and month 6. FA performed pre-op and at week 6 was divided into three leakage patterns. OCT performed pre-op, at week 6 and month 6 was qualitatively divided into three types. Macular thickness was measured in three circular fields (central subfield, inner and outer circle) from the macular maps.

    There was no statistically significant difference in VA before surgery, at day 7 or at 6 months, but at 6 weeks there was a significant difference with lower VA in the diabetic group. Six percent of control and 12% of diabetic eyes developed a clinical CME defined as a loss of > 5 letters between day 7 and week 6. Incidence of FA leakage was 23% in control and 76% in diabetic eyes. At 6 weeks, 20% of control and 44% of the diabetic eyes had qualitative changes on OCT. A statistically significant increase in thickness was observed for all three macular areas in both groups, part of it remaining at 6 months. There were, however, no differences in central macular thickness between the groups at any visit. Retinal thickening had poor correlation with VA.

    The final visual outcome in eyes with mild to moderate retinopathy, without previous ME, is as good as in normal eyes, but an increased frequency of macular changes may protract recovery of full vision. Changes on OCT or FA are often seen without any obvious effect on VA. OCT is as good as FA at detecting a clinical CME, and is the technique recommended for follow-up before FA is considered.

    Nyckelord
    Visual outcome, Phacoemulsification, Cystoid macular edema, Optical coherence tomography, Fluorescein angiography, Sub-clinical macular thickening
    Nationell ämneskategori
    Medicin och hälsovetenskap
    Forskningsämne
    Oftalmiatrik
    Identifikatorer
    urn:nbn:se:uu:diva-128901 (URN)10.1007/s00417-010-1484-9 (DOI)000288454200003 ()20827486 (PubMedID)
    Tillgänglig från: 2010-08-03 Skapad: 2010-08-02 Senast uppdaterad: 2018-05-28Bibliografiskt granskad
    Ladda ner fulltext (pdf)
    FULLTEXT01
  • 41.
    Eriksson, Urban
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Alm, Albert
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Larsson, Eva
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Is quantitative spectral-domain superior to time-domain optical coherence tomography (OCT) in eyes with age-related macular degeneration?2012Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 90, nr 7, s. 620-627Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose: The aim of this study was to determine the variability of macular map measurements, for two generations of optical coherence tomography (OCT) instruments, in eyes with wet age related macular degeneration (AMD) and low visual acuity. Methods: Patients were examined with Stratus OCT and Cirrus HD-OCT. The macular thickness was assessed with the 'macular thickness map scan' and 'fast protocol' in Stratus and with the 512 × 128 and 200 × 200 cube protocols in Cirrus OCT. Two measurements were taken one directly after the other, at the first visit to analyse repeatability. Approximately 1 week later, a third measurement was taken to analyse reproducibility. In Cirrus OCT, a manual correction of foveal location was also performed. Repeatability and reproducibility were calculated as a coefficient of variance (CoV) and a coefficient of repeatability/reproducibility. Results: Repeatability for central macular thickness (expressed as CoV) was about three per cent for all protocols, and the coefficient of repeatability between 34 and 54 μm. Reproducibility (also expressed as CoV) was between four to seven per cent and coefficient of repeatability between 64 and 89 μm. After manual adjustment of foveal location in Cirrus OCT, the coefficient of repeatability improved to 12-18 μm, and the coefficient of reproducibility to 44-47 μm. Conclusions: In eyes affected by wet AMD, there were small differences in repeatability and reproducibility when comparing quantitative maps in Stratus and Cirrus OCT. However, when the software for manual correction of foveal position in Cirrus OCT was used, the variability decreased markedly, and the repeatability was close to what had been reported in normal eyes, demonstrating a significant, potential advantage of spectral-domain over time-domain OCT.

  • 42.
    Farhoudi, Daniel B.
    et al.
    Karolinska Inst, St Erik Eye Hosp, Div Ophthalmol & Vis, Dept Clin Neurosci, Stockholm, Sweden.
    Behndig, Anders
    Umea Univ Hosp, Dept Clin Sci Ophthalmol, Umea, Sweden.
    Mollazadegan, Kaziwe
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Montan, Per
    Karolinska Inst, St Erik Eye Hosp, Div Ophthalmol & Vis, Dept Clin Neurosci, Stockholm, Sweden.
    Lundstrom, Mats
    Lund Univ, Dept Clin Sci, Ophthalmol, Fac Med, Lund, Sweden.
    Kugelberg, Maria
    Karolinska Inst, St Erik Eye Hosp, Div Ophthalmol & Vis, Dept Clin Neurosci, Stockholm, Sweden.
    Spectacle use after routine cataract surgery and vision-related activity limitation2018Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 96, nr 6, s. 582-585Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose To explore the relationship between acquisition of new spectacles after routine cataract surgery and vision-related activity limitation (VRAL) postoperatively. MethodsResultsThis cohort study with intervention (survey) included 1329 patients in Sweden who had undergone a second-eye cataract surgery during March 2013. Data from the Swedish National Cataract Register were used, including evaluations of VRAL through the Catquest-9SF questionnaire before and 3months after cataract surgery. Five months after the second-eye surgery, patients completed another five-item questionnaire about spectacle use preoperatively and postoperatively including an item on surgeons' advice about the need for spectacles. These responses were linked to the Rasch-analysed Catquest-9SF data to identify correlations with VRAL. A total of 1239 patients finally participated in the study after excluding those who did not fulfil the inclusion criteria. Patients who were advised about the need for spectacles postoperatively (n=387) had a greater (p=0.039) improvement in the postoperative VRAL compared to patients who were not advised (n=691). Patients who obtained new spectacles postoperatively (n=512) also had greater improvement (p=0.032) compared to those who did not (n=724). ConclusionThe average improvements in the VRAL after surgery were significantly higher for patients who obtained new distance spectacles postoperatively and for patients who were informed about the need for spectacles by their practitioners.

  • 43.
    Freitag, Fabio Batista
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap. Univ Fed Rio Grande do Norte, Lab Sensory Ecol, BR-59072970 Natal, RN, Brazil; Univ Fed Rio Grande do Norte, Lab Adv Study Primates, BR-59072970 Natal, RN, Brazil.
    Pessoa, Daniel Marques Almeida
    Univ Fed Rio Grande do Norte, Lab Sensory Ecol, BR-59072970 Natal, RN, Brazil; Univ Fed Rio Grande do Norte, Lab Adv Study Primates, BR-59072970 Natal, RN, Brazil.
    Effect of luminosity on color discrimination of dichromatic marmosets (Callithrix jacchus)2012Ingår i: Optical Society of America. Journal A: Optics, Image Science, and Vision, ISSN 1084-7529, E-ISSN 1520-8532, Vol. 29, nr 2, s. A216-22Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Psychophysical data have shown that under mesopic conditions cones and rods can interact, improving color vision. Since electrophysiological data have suggested that rods of dichromatic marmosets appear to be active at higher luminance, we aimed to investigate the effect of different levels of sunlight on the foraging abilities of male dichromatic marmosets. Captive marmosets were observed under three different conditions, with respect to their performance in detecting colored food items against a green background. Compared to high and low light intensities, intermediate luminosities significantly increased detection of orange targets by male dichromats, an indication of rod intrusion.

  • 44. Frennesson, Christina I.
    et al.
    Wadelius, Claes
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Medicinsk genetik.
    Nilsson, Sven Erik G.
    Best vitelliform macular dystrophy in a Swedish family: genetic analysis and a seven-year follow-up of photodynamic treatment of a young boy with choroidal neovascularization2014Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 92, nr 3, s. 238-242Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Abstract. Purpose: To determine the mutation in a Swedish family with Best disease (vitelliform macular dystrophy; VMD) and to investigate the short- and long-term effects of photodynamic treatment (PDT) on subretinal neovascularization in a young boy. Methods: The five members of three generations of a family with VMD underwent a thorough ophthalmological examination, including best-corrected visual acuity (VA), visual field, colour vision, biomicroscopy of the posterior segment (dilated), fundus photography and electro-oculography (EOG). For the proband, an eleven-year-old boy, his father and grandfather, dark adaptation test, angiography and electroretinography (ERG) were also performed. After PCR amplification, the genotype was determined by cleavage with restriction enzyme, specific for the W93C allele. Results: Four family members had an abnormal EOG response. All showed the W93C mutation in the VMD2 gene. Visual acuity ranged from 20/20 to 20/250. The fundus manifestations varied from minor pigmentary changes over egg yolk-like lesions to chorioretinal atrophy, and fluorescein angiography showed corresponding pathology. In the proband, VA decreased during follow-up from 0.5 (20/40) to 0.08 (20/250) due to a subfoveal neovascularization with haemorrhage, and PDT with visudyne was begun. The haemorrhage resolved within 2 months, and after three treatments, VA had increased to 0.25 (20/80). One year later, acuity had improved to 0.5 (20/40), and this result was stable throughout the 7 years of the follow-up. Conclusion: The mutation was determined to be W93C, the most common mutation in VMD in Sweden. In an eleven-year-old boy with subretinal neovascularization, PDT seemed to be beneficial also in a long-term follow-up.

  • 45.
    Galichanin, Konstantin
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Exposure to subthreshold dose of UVR-B induces apoptosis in the lens epithelial cells and does not in the lens cortical fibre cells2017Ingår i: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 95, nr 8, s. 834-838Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose: The aim of this study was to investigate in which part of the lens invivo exposure to subthreshold dose of UVR-B radiation induces apoptosis.

    Methods: Twenty 6-week-old female albino Sprague-Dawley rats were exposed to subthreshold dose (1kJ/m(2)) of UVR-B unilaterally and killed at 120hr after exposure. Lenses were enucleated and dissected on three regions: the lens epithelium, the cortex and the nucleus. The lens nucleus then was removed. Apoptosis markers p53 and caspase 3 were used to study apoptosis in the lens regions. qRT-PCR and Western blot were utilized to analyse the lenses.

    Results: TP53 and CASP3 mRNA expressions are increased in exposed lenses, both in the lens epithelium and in the cortex regions, in relation to non-exposed lenses. Expression of p53 protein is increased in exposed lens epithelium in relation to non-exposed lens epithelium. Caspase 3 protein is expressed in exposed lens epithelial cells, while it is not expressed in non-exposed lens epithelial cells. p53 and caspase 3 proteins are not expressed in either exposed nor non-exposed lens fibre cells.

    Conclusion: Exposure to UVR-B increases mRNA transcription of apoptosis marker p53 invivo in both regions of the lens and of apoptosis marker caspase 3 in the lens cortex. Exposure to UVR-B increases p53 and caspase 3 proteins expression just in the lens epithelium. In vivo exposure to subthreshold dose of UVR-B induces apoptosis in the lens epithelial cells and does not in the lens fibre cells.

  • 46.
    Galichanin, Konstantin
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Svedlund, Jessica
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kirurgiska vetenskaper, Endokrinkirurgi.
    Söderberg, Per
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Kinetics of GADD45a, TP53 and CASP3 gene expression in the rat lens in vivo in response to exposure to double threshold dose of UV-B radiation2012Ingår i: Experimental Eye Research, ISSN 0014-4835, E-ISSN 1096-0007, Vol. 97, nr 1, s. 19-23Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The purpose of the present study was to investigate the evolution of expression of mRNA message for the genes for the genome stress sensor GADD45α, the apoptosis initiator TP53 and the apoptosis executor CASP3 in the rat lens in vivo in response to exposure to UVR around 300 nm. Forty six week old female albino Sprague-Dawley rats were unilaterally exposed to double threshold dose for cataract induction, 8 kJ/m2 (8.9 W/m2 for 15 min), of UVR (λmax = 300 nm). The animals were sacrificed at 1, 5, 24 and 120 h following exposure to UVR-B. For each of the GADD45α, TP53 and CASP3 genes, respectively, mRNA expression in the lenses was measured by quantitative RT-PCR. It was found that expression of mRNA for GADD45α transiently increases between 5 and 24 h after exposure. TP53 is slightly downregulated in exposed lenses at 1 and 5 h after exposure and thereafter the mRNA expression increases with a constant rate of 9.4∗10−3 rel. units/h to a 1.8 fold increase at 120 h after exposure. Expression of mRNA for CASP3 is downregulated at 1, 5 and 24 h after in vivo exposure and then increases with a constant rate of 4.7∗10−3 rel. units/h, upto a 1.3 fold upregulation at 120 h. Double threshold dose of UVR, for short delay onset of cataract, in vivo causes a transient upregulation of the stress sensor GADD45α, a concurrent downregulation of TP53 and CASP3, followed by a constant upregulation of TP53 that precedes a constant upregulation of CASP3.

  • 47.
    Galichanin, Konstantin
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Yu, Zhaohua
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Söderberg, Per
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Oftalmiatrik.
    Upregulation of GADD45a, TP53 and CASP3 mRNA expression in the rat lens after in vivo exposure to sub-threshold exposure to UVR B2014Ingår i: Journal of Ocular Biology, ISSN 2334-2838, Vol. 2, nr 1, s. 5-Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose:

    The objective of the present study was to investigate the evolution of mRNA expression of the stress sensor GADD45α, the apoptosis initiator TP53 and the apoptosis executor CASP3 in the rat lens after in vivo exposure to sub-threshold dose of UVR-B.

    Methods:

    Forty six-week-old female albino Sprague-Dawley rats were unilaterally exposed to a sub-threshold dose, 1 kJ/m2 (1.1 W/m2 for 15 min), of UVR (λmax = 300 nm). Anesthetized and dilated eyes were exposed to UVB radiation. The animals were sacrificed at 1, 5, 24 and 120 h post-exposure. mRNA expression of the GADD45α, TP53 and CASP3 genes in the lenses was measured by quantitative RT‑PCR, and fold change in mRNA expression between exposed and unexposed lenses was calculated.

    Results:

    mRNA expression for GADD45α increased to a 1.2 fold change at 1 h after exposure and then returned to no change at 120 h. mRNA expression for TP53 increased with a regression coefficient of 0.04 h-1 to a maximum of 1.67 fold change. mRNA expression for CASP3 increased with a regression coefficient of 4.5 x10-3 rel. units/h to a 1.46 fold change at 120 h after exposure.

    Conclusions:

    A sub-threshold in vivo exposure to UVR-B causes a transient upregulation of the stress sensor GADD45α at 1 h after exposure, a saturating upregulation of TP53 and a subsequent constant upregulation of CASP3 in the rat lens.

  • 48.
    Ghaderi Berntsson, Shala
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Kristoffersson, Anna
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Daniilidou, Makrina
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Dahl, Niklas
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi.
    Ekström, Curt
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Markström, Agneta
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper.
    Niemelä, Valter
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Partinen, Markku
    Hallböök, Finn
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Landtblom, Anne-Marie
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap.
    Aniridia with PAX6 mutations and narcolepsy2020Ingår i: Journal of Sleep Disorders: Treatment and care, ISSN 2325-9639, E-ISSN 2325-9639, Vol. 29, nr 1, s. 1-7Artikel i tidskrift (Refereegranskat)
  • 49. Gole, Glen A.
    et al.
    Ells, Anna L.
    Katz, Ximena
    Holmström, Gerd
    Uppsala universitet, Medicinska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap. Oftalmiatrik.
    Fielder, Alistair R.
    Capone, Antonio
    Flynn, John T.
    Good, William G.
    Holmes, Jonathan M.
    McNamara, J. Arch
    Palmer, Earl A.
    Quinn, Graham E.
    Shapiro, Michael J.
    Trese, Michael G. J.
    Wallace, David K.
    The International Classification of Retinopathy of Prematurity Revisited: An International Committee for the Classification of Retinopathy of Prematurity2005Ingår i: Arch Ophthalmol, Vol. 123, s. 991-999Artikel i tidskrift (Refereegranskat)
  • 50.
    Granstam, Elisabet
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Centrum för klinisk forskning, Västerås. Vastmanland Cty Hosp Vasteras, Ophthalmol, Vasteras, Sweden..
    Sjovall, Kersti
    Vastmanland Cty Hosp Vasteras, Ophthalmol, Vasteras, Sweden..
    Paul, Anna
    Vastmanland Cty Hosp Vasteras, Ophthalmol, Vasteras, Sweden..
    Eriksson, Laila
    Vastmanland Cty Hosp Vasteras, Ophthalmol, Vasteras, Sweden..
    Change of treatment strategy for wet AMD from PRN to Treat-and-Extend: 6 months experience from a Swedish county hospital2015Ingår i: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 56, nr 7, artikel-id 5366Artikel i tidskrift (Refereegranskat)
1234 1 - 50 av 194
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