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  • 1.
    Hidalgo, Christian
    et al.
    Univ Andres Bello, Fac Ecol & Recursos Nat, Escuela Med Vet, Santiago, Chile..
    Pia Garcia, Maria
    Univ Andres Bello, Fac Ecol & Recursos Nat, Escuela Med Vet, Santiago, Chile..
    Stoore, Caroll
    Univ Andres Bello, Fac Ecol & Recursos Nat, Escuela Med Vet, Santiago, Chile..
    Pablo Ramirez, Juan
    Univ Andres Bello, Fac Ecol & Recursos Nat, Escuela Med Vet, Santiago, Chile..
    Monteiro, Karina Mariante
    Univ Fed Rio Grande Sul UFRGS, Lab Genom Estrutural & Func, Porto Alegre, RS, Brazil.;Univ Fed Rio Grande Sul UFRGS, Lab Biol Mol Cestodeos, Porto Alegre, RS, Brazil..
    Hellman, Ulf
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Ludwig Institute for Cancer Research.
    Zaha, Arnaldo
    Univ Fed Rio Grande Sul UFRGS, Lab Genom Estrutural & Func, Porto Alegre, RS, Brazil.;Univ Fed Rio Grande Sul UFRGS, Lab Biol Mol Cestodeos, Porto Alegre, RS, Brazil..
    Ferreira, Henrique Bunselmeyer
    Univ Fed Rio Grande Sul UFRGS, Lab Genom Estrutural & Func, Porto Alegre, RS, Brazil.;Univ Fed Rio Grande Sul UFRGS, Lab Biol Mol Cestodeos, Porto Alegre, RS, Brazil..
    Galanti, Norbel
    Univ Chile, Fac Med, Inst Ciencias Biomed, Programa Biol Celular & Mol, Santiago 7, Chile..
    Landerer, Eduardo
    Univ Andres Bello, Fac Med, Escuela Med, Santiago, Chile..
    Paredes, Rodolfo
    Univ Andres Bello, Fac Ecol & Recursos Nat, Escuela Med Vet, Santiago, Chile..
    Proteomics analysis of Echinococcus granulosus protoscolex stage2016In: Veterinary parasitology, ISSN 0304-4017, E-ISSN 1873-2550, Vol. 218, p. 43-45Article in journal (Refereed)
    Abstract [en]

    Echinococcus granulosus protoscolex proteins were separated using two-dimensional electrophoresis and then identified using mass spectrometry; we identified 61 proteins, 28 which are newly described of which 4 could be involved in hydatid cyst fertility molecular mechanisms.

  • 2. Lebbad, Marianne
    et al.
    Mattsson, Jens G
    Christensson, Bodil
    Ljungström, Bitte
    Backhans, Annette
    Andersson, Jan O.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organism Biology, Molecular Evolution.
    Svärd, Staffan G.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
    From mouse to moose: multilocus genotyping of Giardia isolates from various animal species.2010In: Veterinary parasitology, ISSN 0304-4017, E-ISSN 1873-2550, Vol. 168, no 3-4, p. 231-239Article in journal (Refereed)
    Abstract [en]

    Giardia intestinalis is a protozoan parasite that consists of seven genetically distinct assemblages (A to G). Assemblage A and B parasites have been detected in a wide range of animals including humans, while the other assemblages (C to G) appear to have a narrower host range. However, the knowledge about zoonotic transmission of G. intestinalis is limited. To address this question, 114 Giardia isolates from various animals in Sweden including pets, livestock, wildlife and captive non-human primates were investigated by a sequence-based analysis of three genes (beta-giardin, glutamate dehydrogenase and triose phosphate isomerase). Assemblage A infections were detected in nine ruminants, five cats and one dog, while three sheep were infected with both assemblages A and E. Multilocus genotypes (MLGs) were defined for assemblage A, and three of these MLGs have previously been detected in Giardia isolates from humans. The newly described sub-assemblage AIII, until now reported mainly in wild hoofed animals, was found in one cat isolate. Assemblage B occurred in three monkeys, one guinea pig and one rabbit. The rabbit isolate exhibited sequences at all three loci previously detected in human isolates. The non-zoonotic assemblages C, D, E, F or G were found in the remaining 83 G. intestinalis isolates, which were successfully amplified and genotyped, generating a wide variety of both novel and known sub-genotypes. Double peaks in chromatograms were seen in assemblage B, C, D and E isolates but were never observed in assemblage A, F and G isolates, which can reflect differences in allelic sequence divergence. No evidence of genetic exchange between assemblages was detected. The study shows that multilocus genotyping of G. intestinalis is a highly discriminatory and useful tool in the determination of zoonotic sub-groups within assemblage A, but less valuable for subtyping assemblages B, C, D and E due to the high frequency of double peaks in the chromatograms. The obtained data also suggest that zoonotic transmission of assemblages A and B might occur to a limited extent in Sweden.

  • 3. Lebbad, Marianne
    et al.
    Svärd, Staffan
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Microbiology.
    Comment on article by Eligio-Garcia et al. entitled "Frequency of Giardia intestinalis assemblages isolated from dogs and humans in a community from Culiacan, Sinaloa, Mexico using beta-giardin restriction gene"2008In: Veterinary parasitology, ISSN 0304-4017, E-ISSN 1873-2550, Vol. 158, no 1-2, p. 159-160Article in journal (Refereed)
  • 4. Scandrett, W Brad
    et al.
    Haines, Deborah M
    Parker, Sarah E
    Robinson, Yves
    St. Hyacinthe Laboratory, Canadian Food Inspection Agency, 3400 Casavant Boulevard W, St. Hyacinthe, Quebec, Canada.
    Forbes, Lorry B
    Brandt, Jef
    Geerts, Stanny
    Dorny, Pierre
    Gajadhar, Alvin A
    Validation of an immunohistochemical assay for bovine cysticercosis, with comparison to a standard histological method2012In: Veterinary parasitology, ISSN 0304-4017, E-ISSN 1873-2550, Vol. 186, no 3-4, p. 301-311Article in journal (Refereed)
    Abstract [en]

    The larval stage (syn Cysticercus bovis) of the human tapeworm Taenia saginata causes cysticercosis in cattle, which has both aesthetic and food safety implications to consumers of beef. A monoclonal antibody-based immunohistochemical (IHC) assay developed to improve postmortem diagnosis of this parasite and a standard histological method were assessed to determine their fitness for intended use. Sections from 169 known-positive specimens of T. saginata from experimentally or naturally infected cattle, and from 30 known-negative specimens and lesions of various etiologies from non-infected cattle, were tested. The IHC assay identified significantly more known positive bovine cysticerci than the histological method (91.7% and 38.5%, respectively). Positive IHC staining occurred on sections from other cestode species, but should not affect the diagnostic specificity of this assay for bovine cysticercosis, due to the different host and/or tissue preferences amongst these parasites. Use of the IHC assay should improve the reliability of diagnosing lesions caused by degenerated cysticerci, facilitating more effective and efficient control of bovine cysticercosis.

  • 5. Van Lith, Lindy
    et al.
    Soba, Barbara
    Vizcaino, Vivian Villalba
    Svärd, Staffan
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Microbiology.
    Sprong, Hein
    Tosini, Fabio
    Pozio, Edoardo
    Caccio, Simone M.
    A real-time assemblage-specific PCR assay for the detection of Giardia duodenalis assemblages A, B and E in fecal samples2015In: Veterinary parasitology, ISSN 0304-4017, E-ISSN 1873-2550, Vol. 211, no 1-2, p. 28-34Article in journal (Refereed)
    Abstract [en]

    Giardiosis is a common gastrointestinal infection caused by the flagellate Giardia duodenalis, and affects both humans and animals, worldwide. Animals are infected with both zoonotic and host-specific G. duodenalis assemblages, and their role in the transmission of the infection to humans has been a subject of intense research and debate. Conventional PCR assays are appropriate to determine G. duodenalis assemblages, but lack sensitivity for the detection of mixed infections. Previous surveys demonstrated the occurrence of mixed infections with G. duodenalis assemblage A and B in humans, and with assemblages A and E in cattle, but are likely to be underestimated. In this study, we designed a set of assemblage-specific primers by exploiting sequence variability in homologous genes from assemblages A, B and E. Primers were designed to amplify fragments of different size that generated different melting curves from each assemblage in real-time PCR (rt-PCR) experiments. The assay has been tested on a large panel of human and farm animal isolates, and shown to possess high specificity (no cross reactions observed) and sensitivity (detection limit close to 20 copies). Therefore, this assay can be useful to detect zoonotic and host-specific G. duodenalis assemblages in fecal samples from farm animals, particularly when a large number of samples is to be tested.

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