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  • 1.
    Anger, S
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Caldwell, KD
    Müller, RH
    Characterization of macro molecules, colloids and particles by field-flow fractionation=Characterisierung von Makromolekülen, Kolloiden und Partikeln mit der Field-Flow Fractionation2000In: PHARMAZEUTISCHE INDUSTRIE, ISSN 0031-711X, Vol. 62, no 2, p. 150-156Article in journal (Refereed)
    Abstract [en]

    Field-Flow Fractionation (FFF) is a versatile elution technique capable of simultaneous separation and measurement of macromolecular, colloidal and particulate systems in a size range from a few nanometers to about 100 mu m. This article describes the pri

  • 2.
    B. Ersson, L. Rydén and J.-C. Janson
    Uppsala University, Centre for Surface Biotechnology.
    Introduction to Protein Purification1998In: J.-C. Janson and L. Rydén, (eds.), Protein Purification, Principles, High Resolution Methods and Applications, John Wiley & Sons Inc., New York , 1998, p. 1-40Chapter in book (Other scientific)
  • 3.
    B., Österlund and J.-C. Janson
    Uppsala University, Centre for Surface Biotechnology.
    A Strategic Approach to Protein Purification, Part 11997In: Science Tools, Vol. 2, no 3Article in journal (Refereed)
  • 4.
    B., Österlund and J.-C. Janson
    Uppsala University, Centre for Surface Biotechnology.
    A Strategic Approach to Protein Purification, Part 21998In: Science Tools, Vol. 3, no 1, p. 20-22Article in journal (Refereed)
  • 5. Bacskay, Ivett
    et al.
    Takatsy, Aniko
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Biochemistry and Organic Chemistry, Biochemistry.
    Vegvari, Akos
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Biochemistry and Organic Chemistry, Biochemistry.
    Elfwing, Anders
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Balllagi-Pordany, Andras
    Kilar, Ferenc
    Hjertén, Stellan
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Biochemistry and Organic Chemistry, Biochemistry.
    Universal method for synthesis of artificial gel antibodies by the imprinting approach combined with a unique electrophoresis technique for detection of minute structural differences of proteins, viruses, and cells (bacteria). III: Gel antibodies against cells (bacteria)2006In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 27, no 23, p. 4682-4687Article in journal (Refereed)
    Abstract [en]

    Artificial antibodies in the form of gel granules were synthesized from the monomers acrylamide and N,N'-methylenebisacrylamide by the imprinting method in the presence of Echerichia coli bacteria as template. The electrophoretic migration velocities of the gel antibodies (i) saturated with the antigen (Escherichia coli MRE-600), (ii) freed of the antigen, and (iii) resaturated with bacteria, were determinated by electrophoresis in a rotating narrow-bore tube of 245 mm length and the 2.5 and 9.6 mm inner and outer diameters, respectively. Removal of bacteria from the gel antibodies was made by treatment with enzymes, followed by washing with SDS and buffer. Gel granules becoming charged by adsorption of bacteria move in an electrical field. We obtained a significant selectivity of gel antibodies for E coli MRE-600, since the granules did not interact with Lactococcus lactis; and when E coli BL21 bacteria were added to the gels selective for E coli MRE-600, a significant difference in the migration rate of the complexes formed with the two strains was observed indicating the ability of differentiation between the two strains. The gel antibodies can be used repeatedly. The new imprinting method for the synthesis of artificial gel antibodies against bioparticles described herein, and the classical electrophoretic analysis technique employed, thus represent - when combined - a new approach to distinguish between different types and strains of bacteria. The application area can certainly be extended to cover other classes of cells.

  • 6.
    Ballagi-Pordany A., Belak S.
    Uppsala University, Centre for Surface Biotechnology.
    The use of mimics as internal standards to avoid false negatives in diagnostic PCR1996In: Mol Cell Probes, Vol. 10, no 3, p. 159-164Article in journal (Refereed)
  • 7.
    Ballagi-Pordany A., Wehmann E., Herczeg J., Belak, S. and Lomniczi, B.
    Uppsala University, Centre for Surface Biotechnology.
    Identification and grouping of Newcastle disease virus strains by restriction site analysis of a region from the F gene1996In: Arch Virol, Vol. 141, no 2, p. 243-261Article in journal (Refereed)
  • 8.
    Barinov, S. M.
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Fateeva, L. V.
    Jigarnovski, B. M.
    Orlovski, V. P.
    Oscarsson, S.
    Rustichelli, F.
    Stanic, V.
    Tumanov, S. V.
    Hydroxyapatite-fluoroapatite ceramics: Preparation and Properties2001In: Proceedings 7th Seminar and Meetings; Biomimetic Engineeing June 13-15, 2001, Faenza, Italy, Gruppo editoriale faenza editrice s.p.a. , 2001, p. 153-160Conference paper (Refereed)
  • 9.
    Barnfield-Frej, A.-K., and Hjorth, R.
    Uppsala University, Centre for Surface Biotechnology.
    The use of expanded bed adsorption (EBA) to recover proteins in industrial bioprocessing1998In: Recent Research and Development in Biotechnology and Bioengineering, Vol. 1, p. 357-372Article in journal (Refereed)
  • 10.
    Bascunana C.R., Bjornerot L., Ballagi-Pordany A., Robertsson, J.A. and Belak, S.
    Uppsala University, Centre for Surface Biotechnology.
    Detection of pseudorabies virus genomic sequences in apparently uninfected 'single reactor' pigs1997In: Vet. Microbiol., Vol. 55, no 001-004, p. 37-47Article in journal (Refereed)
  • 11.
    Basinska, T. and Caldwell, K.D.
    Uppsala University, Centre for Surface Biotechnology.
    Colloidal Particles as Immunodiagnostics: Preparation and FFF Characterization1999In: T. Provder, ed, Chromatography of Polymers: Hyphenated and Multidimensional Techniques, American Chemical Society, Washington DC , 1999, p. 162-177Conference paper (Other scientific)
  • 12.
    Batista-Viera, F., Manta, C. and Carlsson, J.
    Uppsala University, Centre for Surface Biotechnology.
    Covalent binding of thiols to thiolsulphinate-containing supports1996In: Biotechnol. Appl. Biochem., Vol. 24, p. 231-239Article in journal (Refereed)
  • 13.
    Belak S., Rivera E., Ballagi-Pordany A., Hanzhong, W., Widen, F., and Soos, T.
    Uppsala University, Centre for Surface Biotechnology.
    Detection of challenge virus in fetal tissues by nested PCR as a test of the potency of a porcine parvovirus vaccine1998In: Vet Res Commun, Vol. 22, no 2, p. 139-146Article in journal (Refereed)
  • 14.
    Benincasa, Maria-Anna
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Caldwell, Karin D.
    Flow FFF of Poly(ethylene oxide): Effect of Carrier Ionic Strength and Composition2001In: Journal of Chromatography A, no 925, p. 159-169Article in journal (Refereed)
  • 15.
    Bergkvist, M.
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Carlsson, J.
    Oscarsson, S.
    Tertiary Structure of Fibronectin Adsorbed to Hydrophobic/Hydrophilic Surfaces Studied with AFM2001In: Proceedings 7th Seminar anf Meetings; Biomimetic Engineering June 13-15, 2001, Faenza, Italy, Gruppo editoriale faenza editrice s.p.a. , 2001, p. 237-245Conference paper (Refereed)
  • 16.
    Berglund, Rolf
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Bergström, Jan
    Belew, Makonnen
    Söderberg, Lennart
    Process for chromatographic separation of peptides and nucleic acids, and new high affinity ion exchange matrix2000Patent (Other (popular scientific, debate etc.))
  • 17.
    Berglund, Rolf
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Bergström, Jan
    Söderberg, Lennart
    Method for separation on cation exchangers2001Patent (Other (popular scientific, debate etc.))
  • 18.
    Bergström, Jan
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Berglund, Rolf
    Söderberg, Lennart
    Matrices for separation and separation exploiting said matrices2000Patent (Other (popular scientific, debate etc.))
  • 19.
    Bergström, Jan
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Söderberg, Lennart
    Liquid chromatography system1998Patent (Other (popular scientific, debate etc.))
  • 20.
    Berna, N., Berna, P., and Oscarsson, S.
    Uppsala University, Centre for Surface Biotechnology.
    Co - Solvent Induced Adsorption and Desorption of Serum Proteins on an Amphiphilic Mercaptomethylenepyridin - Derivatized Agarose Gel1996In: Archives of Biochemistry and Biophysics, Vol. 330, no 1, p. 188-192Article in journal (Refereed)
  • 21.
    Berna, P., Mrabet, N., T., Van Beeumen, J., Devreese, B., Porath, J., Vijayalakshmi, M., A.
    Uppsala University, Centre for Surface Biotechnology.
    Residue accessibility, hydrogen bonding, and molecular recognition: Metal-chelate probing of active site histidines in chymotrypsins1997In: Biochemistry, Vol. 36, no 23, p. 6896-6905Article in journal (Refereed)
  • 22.
    Boden, V., Winzerling, J., Vijayalakshmi, M., A. And Porath, J.
    Uppsala University, Centre for Surface Biotechnology.
    Rapid one-step purification of goat immonuglobulins by immobilized metal ion affinity chromatography1995In: J. Immunolog. Methods, Vol. 181, p. 225-232Article in journal (Refereed)
  • 23.
    Bohner, K.
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Ring, T. A.
    Rapoport, Natalya
    Caldwell, K. D.
    Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Surface Biotechnology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Physical and Analytical Chemistry.
    Fibrinogen adsorption by latex particles coated with various amounts of PEO/PPO/PEO triblock copolymer2002In: J. Biomater. Sci. Polymer Edition, Vol. 13, no 6, p. 733-746Article in journal (Refereed)
  • 24. Bohner, M.
    et al.
    Ring, T. A.
    Caldwell, K. D.
    Uppsala University, Centre for Surface Biotechnology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Physical and Analytical Chemistry.
    Studies on the Effect of Particle Size and Copolymer Polydispersity on the Adsorption of a PEO/PPO/PEO Copolymer on PS Latex Particles2002In: Macromolecules, Vol. 35, p. 6724-6731Article in journal (Refereed)
  • 25.
    Brena, B., Lidholm, J., Batista-Viera, F. and Carlsson, J.
    Uppsala University, Centre for Surface Biotechnology.
    Selective removal of enzymes from Substrate and Products. An alternative to immobilization for enzymes acting on macromolecular or solid substrates1998In: Applied Biochemistry and Biotechnology, Vol. 75, p. 323-341Article in journal (Refereed)
  • 26.
    Bruckner-Lea, C.J., Kimmel, R.J., Janata, J., Conroy, J.F., and Caldwell, K.D.
    Uppsala University, Centre for Surface Biotechnology.
    Electrochemical Studies of Octadecanethiol and Octanethiol Films on Variable Surface Area Mercury Sessile Drops1995In: Electrochimica Acta, Vol. 40, p. 2897-2904Article in journal (Refereed)
  • 27.
    Buijs, J
    et al.
    Uppsala University, Centre for Surface Biotechnology. IONPHYSICS.
    Hagman, C
    Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Materials Science.
    Håkansson, K
    Richter, JH
    Håkansson, P
    Oscarsson, S
    Inter- and intra-molecular migration of peptide amide hydrogens during electrospray ionization2001In: JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, ISSN 1044-0305, Vol. 12, no 4, p. 410-419Article in journal (Refereed)
    Abstract [en]

    The isotopic exchange of amide hydrogens in proteins in solution strongly depends on the surrounding protein structure, thereby allowing structural studies of proteins by mass spectrometry. However, during electrospray ionization (ESI), gas phase processe

  • 28.
    Buijs, J
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Materials Science.
    Håkansson, K.
    Centre for Surface Biotechnology.
    Hagman, C.
    Håkansson, P. and Oscarsson, S.
    A new metod for the accurate determination of the isotopic state of single amide hydrogens within peptides using Fourier transform ion cyclotron resonance mass spectrometry2000In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, Vol. 14, no 19, p. 1751-1756Article in journal (Refereed)
    Abstract [en]

    A new method is presented to accurately determine the probability of having a deuterium or hydrogen atom on a specific amide position within a peptide after deuterium/hydrogen (D/H) exchange in solution. Amide hydrogen exchange has been proven to be a sen

  • 29.
    Buijs, Jos
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology.
    Ramström, Margareta
    Uppsala University, Disciplinary Domain of Science and Technology.
    Danfelter, Mikael
    Larsericsdotter, Helén
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Håkansson, Per
    Oscarsson, Sven
    Localized changes in the structural stability of myoglobin upon adsorption onto silica particles, as studied with hydrogen/deuterium exchange mass spectrometry2003In: Journal of Colloid and Interface Science, ISSN 0021-9797, Vol. 263, no 2, p. 441-448Article in journal (Refereed)
  • 30.
    Caldwell, K. D.
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Carlsson, P.J.E
    Li, J.T.
    Product for Coating Hydrophobic Surfaces to render them Protein Resistant while Permitting Covalent Attachment of Specific Ligands1996Patent (Other (popular scientific, debate etc.))
  • 31.
    Caldwell, K. D.
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Jansson, J.-C.
    80th birthday of Professor Jerker Porath2001In: Chromatographia, Vol. 54, no 7-8, p. 431-432Other (Other scientific)
  • 32.
    Caldwell, Karin
    Uppsala University, Centre for Surface Biotechnology.
    Steric Field Flow Fractionation and the Steric Transition2000In: Field Flow Fractionation Handbook, New York: John Wiley & Sons, Inc., Publications , 2000, p. 79-94Chapter in book (Other scientific)
  • 33.
    Caldwell, Karin D.
    Uppsala University, Centre for Surface Biotechnology.
    Electrical Field Flow Fractionation2000Chapter in book (Other scientific)
  • 34.
    Caldwell, Karin D.
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Tresco, Patrick A.
    Neff, Jennifer
    Composition and method for regulating the adhesion of cells and biomolecules to hydrophobic surfaces2001Patent (Other (popular scientific, debate etc.))
  • 35.
    Caldwell, K.D.
    Uppsala University, Centre for Surface Biotechnology.
    Surface Modifications with Adsorbed PEO-Based Block Copolymers: Physical Characteristics and Biological Use1997In: J.M. Harris, and S. Zalipsky, (eds.), Chemistry and Biological Applications of Polyethylene Glycol, American Chemical Society, Washington , 1997, p. 400-419Chapter in book (Other scientific)
  • 36.
    Caldwell, K.D.
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Carlsson, P.J.E.
    Li, J.T.
    Coating of Hydrophobic Surfaces to Render Them Protein Resistant while Permitting Covalent Attachment of Specific Ligands1998Patent (Other (popular scientific, debate etc.))
  • 37.
    Carlsson, J., Batista-Viera, F., and Rydén, L.
    Uppsala University, Centre for Surface Biotechnology.
    Covalent Chromatography1998In: Janson J.-C., Rydén, L., (eds.), Protein Purification: Principles, High-Resolution Methods, and Applications, Wiley-VCH, New York , 1998, p. 343-373Chapter in book (Other scientific)
  • 38.
    Chaga, G., Guzman, R., Porath, J.
    Uppsala University, Centre for Surface Biotechnology.
    A new method of synthesizing biopolymeric affinity ligands1997In: Biotechnology and Applied Biochemistry, Vol. 26, no 1, p. 007-013Article in journal (Refereed)
  • 39.
    Chen, Q. and Caldwell, K.D.
    Uppsala University, Centre for Surface Biotechnology.
    Field-Flow Fractionation in the Determination of Rates of Surfactant Adsorption to Colloidal Substrates1997In: J. Liq, Chrom. & Rel. Tech., Vol. 20, p. 2509-2524Article in journal (Refereed)
  • 40.
    Choulier, Laurence
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Laune, Daniel
    Orfanoudakis, Georges
    Wlad, Hanna
    Janson, Jan-Christer
    Granier, Claude
    Altschuh, Danièle
    Delineation of a linear epitope by multiple peptide synthesis and phage display2001In: JOURNAL OF IMMUNOLOGICAL METHODS, ISSN 0022-1759, Vol. 249, no 1-2, p. 253-264Article in journal (Refereed)
    Abstract [en]

    Two different approaches, the phage display technique and the Spot peptide synthesis on cellulose membranes, were used to identify sequences recognized by Fab 57P specific for tobacco mosaic virus protein (TMVP), and define the preferred chemical composit

  • 41.
    Conroy, J.F., Caldwell, K.D., Bruckner-Lea, C.J., and Janata, J
    Uppsala University, Centre for Surface Biotechnology.
    An Examination of Surface Waving on Mercury Film Electrodes Using Scanning Tunneling Microscopy1995In: Electrochimica Acta, Vol. 40, p. 2927-2934Article in journal (Refereed)
  • 42.
    Dunkel, M., Tri, N., Beckett, R., and Caldwell, K.D.
    Uppsala University, Centre for Surface Biotechnology.
    Electrical FFF: A Tool for Characterization of Colloidal Adsorption Complexes1997In: J. Microcol. Sep., Vol. 9, p. 177-183Article in journal (Refereed)
  • 43.
    Díaz, T., Ståhl, U., Batista-Viera, F. and Carlsson, J.
    Uppsala University, Centre for Surface Biotechnology.
    Reversible immobilization of chemically modified Pullulanase1995In: Biotechnology Techniques, Vol. 9, no 7, p. 533-538Article in journal (Refereed)
  • 44.
    Egyed L., Ballagi-Pordany A., Bartha A. and Belak, S.
    Uppsala University, Centre for Surface Biotechnology.
    Studies of in vivo distribution of bovine herpesvirus type 4 in the natural host1996In: J Clin Microbiol, Vol. 34, no 5, p. 1091-1095Article in journal (Refereed)
  • 45.
    Ehteshami, G.R., Porath, J. and Guzman, R.Z.
    Uppsala University, Centre for Surface Biotechnology.
    Interactions and Applications of Soluble Heterobifunctional Affinity Chelating Polymers in Immobilized Metal Affinity Chromatography1996In: J. Mol Recog., Vol. 9, p. 733-737Article in journal (Refereed)
  • 46.
    Elvander M., Baule C., Persson M., Egyed, L., Ballagi-Pordany, A., Belak, S. and Alenius, S.
    Uppsala University, Centre for Surface Biotechnology.
    An experimental study of a concurrent primary infection with bovine respiratory syncytial virus (BRSV) and bovine viral diarrhoea virus (BVDV) in calves1998In: Acta Vet Scand, Vol. 39, no 2, p. 251-264Article in journal (Refereed)
  • 47.
    Elvander M., Vilcek S., Baule C., Uttenthal, A., Ballagi-Pordany, A. and Belak, S.
    Uppsala University, Centre for Surface Biotechnology.
    Genetic and antigenic analysis of the G attachment protein of bovine respiratory syncytial virus strains1998In: J Gen Virol, Vol. 79, no 12, p. 2939-2946Article in journal (Refereed)
  • 48.
    Englund, S
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Bolske, G
    Ballagi-Pordany, A
    Johansson, KE
    Detection of Mycobacterium avium subsp paratuberculosis in tissue samples by single, fluorescent and nested PCR based on the IS900 gene2001In: VETERINARY MICROBIOLOGY, ISSN 0378-1135, Vol. 81, no 3, p. 257-271Article in journal (Refereed)
    Abstract [en]

    The aim of this study was to determine if fluorescent PCR could be used instead of nested PCR, for the detection of Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) in clinical specimens, to improve the sensitivity without increasing the

  • 49.
    Erenpreisa, Je
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Ivanov, A
    Dekena, G
    Vitina, A
    Krampe, R
    Uppsala University, Centre for Surface Biotechnology.
    Freivalds, T
    Selivanova, G
    Roach, H.I
    Arrest in Metaphase and Anatomy of Mitotic Catastrophe: Mild Heat Shock in Two Human Osteosarcoma Cell Lines2000In: Cell Biology International, Vol. 24, no 2, p. 61-70Article in journal (Refereed)
  • 50.
    Erenpreisa, Je., Krampe, R., Dekena, G., Vitina A., Freivalds, T., Fringes, B.
    Uppsala University, Centre for Surface Biotechnology.
    Interphase and Mitotic (delayed) Cell Death in Heat Shock Treated Osteosarcoma Cell Line (Hos Te85)1998In: Deutsche Gesellschaft für Pathologic. Kassel Abstr., Vol. 59, p. 002-006Article in journal (Refereed)
1234 1 - 50 of 161
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