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  • 1.
    Fornell, Anna
    et al.
    Department of Biomedical Engineering, Lund University, Sweden.
    Cushing, Kevin
    Department of Biomedical Engineering, Lund University, Lund, Sweden.
    Nilsson, Johan
    Department of Biomedical Engineering, Lund University, Lund, Sweden.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Intra-droplet acoustic separation of two particle species in a droplet microfluidic system2017Conference paper (Refereed)
  • 2.
    Fornell, Anna
    et al.
    Lund University.
    Garofalo, Fabio
    Lund University.
    Nilsson, Johan
    Lund University.
    Bruus, Henrik
    Technical University of Denmark.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund University.
    Intra-droplet acoustic particle focusing: simulations and experimental observations2018In: Microfluidics and Nanofluidics, ISSN 1613-4982, E-ISSN 1613-4990, Vol. 22, no 75Article in journal (Refereed)
    Abstract [en]

    The aim of this paper is to study resonance conditions for acoustic particle focusing inside droplets in two-phase microfluidic systems. A bulk acoustic wave microfluidic chip was designed and fabricated for focusing microparticles inside aqueous droplets (plugs) surrounded by a continuous oil phase in a 380-μm-wide channel. The quality of the acoustic particle focusing was investigated by considering the influence of the acoustic properties of the continuous phase in relation to the dispersed phase. To simulate the system and study the acoustic radiation force on the particles inside droplets, a simplified 3D model was used. The resonance conditions and focusing quality were studied for two different cases: (1) the dispersed and continuous phases were acoustically mismatched (water droplets in fluorinated oil) and (2) the dispersed and continuous phases were acoustically matched (water droplets in olive oil). Experimentally, we observed poor acoustic particle focusing inside droplets surrounded by fluorinated oil while good focusing was observed in droplets surrounded by olive oil. The experimental results are supported qualitatively by our simulations. These show that the acoustic properties (density and compressibility) of the dispersed and continuous phases must be matched to generate a strong and homogeneous acoustic field inside the droplet that is suitable for high-quality intra-droplet acoustic particle focusing.

  • 3.
    Fornell, Anna
    et al.
    Lund University.
    Garofalo, Fabio
    Lund University.
    Nilsson, Johan
    Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund University.
    Acoustophoretic particle manipulation in droplet microfluidics at higher resonance modes2016Conference paper (Refereed)
    Abstract [en]

    In this paper we investigate focusing of microparticles in the presence of multiple pressure nodes inside aqueous droplets by using bulk acoustic waves. The microfluidic chip s for droplet generation and particle encapsulation (within the droplets) were fabricated using anisotropic wet - etching of a silicon wafer. Subsequently, piezoelectric transducers featuring different thicknesses were glued on the chips to build the final devices. The transducer thicknesses were chosen as to match the acoustic resonances of the embedded micro channel at the fundamental frequency, the first and the second harmonics. The actuation of the devices at the first three resonance modes enabled the positioning of the microparticles in one, two or three bands, in accordance with the presence of pressure nodes within the droplet contained in the microchannel. This acoustic particle manipulation technique opens up for new possibilities to perform biological assays using droplet microfluidic platforms.

  • 4.
    Fornell, Anna
    et al.
    Lund University.
    Garofalo, Fabio
    Lund University.
    Nilsson, Johan
    Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund University.
    Experimental investigation of resonance conditions for particle focusing in droplet acoustofluidics2016Conference paper (Refereed)
    Abstract [en]

    We have experimentally studied the conditions for acoustic particle focusing inside aqueous droplets in two-phase-systems, and confirmed our findings by theoretical analysis. The results show that the acoustic properties of the two fluids have to be matched to achieve strong acoustic resonance and focusing in the system.

  • 5.
    Fornell, Anna
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Johannesson, Carl
    Lund University.
    Searle, Sean
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. National University of Singapore.
    Happstadius, Axel
    Lund University.
    Nilsson, Johan
    Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Acoustic trapping: a non-contact method to handle cell-laden hydrogel droplets in a microchannel2019Conference paper (Other academic)
  • 6.
    Fornell, Anna
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Johannesson, Carl
    Lund University, Lund, Sweden.
    Searle, Sean
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. National University of Singapore, Singapore.
    Happstadius, Axel
    Lund University, Lund, Sweden.
    Nilsson, Johan
    Lund University, Lund, Sweden.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Trapping of cell-laden hyaluronic acid-acrylamide hydrogel droplets using bulk acoustic waves2019Conference paper (Refereed)
    Abstract [en]

    In this paper an acoustofluidic system to trap hydrogel droplets is shown. The presented trapping method is label-free, biocompatible and operated in non-contact mode. The results show that the droplets can be trapped at flow rates up to 76 µL/min which corresponds to an average flow speed of 3.2 mm/s. Moreover, it is shown that the droplets can be trapped for several hours, thus allowing for studies of the encapsulated cells over time. An application of the system is shown by performing on-chip cell nuclei staining. 

  • 7.
    Fornell, Anna
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Johannesson, Carl
    Lund University.
    Searle, Sean S.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Natl Univ Singapore, Dept Biomed Engn, Fac Engn,.
    Happstadius, Axel
    Lund University.
    Nilsson, Johan
    Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    An acoustofluidic platform for non-contact trapping of cell-laden hydrogel droplets compatible with optical microscopy2019In: Biomicrofluidics, ISSN 1932-1058, E-ISSN 1932-1058, Vol. 13, article id 044101Article in journal (Refereed)
    Abstract [en]

    Production of cell-laden hydrogel droplets as miniaturized niches for 3D cell culture provides a new route for cell-based assays. Such production can be enabled by droplet microfluidics and here we present a droplet trapping system based on bulk acoustic waves for handling hydrogel droplets in a continuous flow format. The droplet trapping system consists of a glass capillary equipped with a small piezoelectric transducer. By applying ultrasound (4 MHz), a localized acoustic standing wave field is generated in the capillary, trapping the droplets in a well-defined cluster above the transducer area. The results show that the droplet cluster can be retained at flow rates of up to 76 mu l/min, corresponding to an average flow speed of 3.2 mm/s. The system allows for important operations such as continuous perfusion and/or addition of chemical reagents to the encapsulated cells with in situ optical access. This feature is demonstrated by performing on-chip staining of the cell nuclei. The key advantages of this trapping method are that it is label-free and gentle and thus well-suited for biological applications. Moreover, the droplets can easily be released on-demand, which facilitates downstream analysis. It is envisioned that the presented droplet trapping system will be a valuable tool for a wide range of multistep assays as well as long-term monitoring of cells encapsulated in gel-based droplets.

  • 8.
    Fornell, Anna
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Liu, Zhenhua
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Improved acoustic particle enrichment in droplets by optimising the droplet split design2019Conference paper (Other academic)
    Abstract [en]

    Droplet microfluidics has emerged as a valuable platform for miniaturisation of biological experiments on-chip. In droplet microfluidic chips monodisperse droplets containing cells or other bioparticles can be generated at high throughput, and each droplet can be used as an isolated reaction chamber for individual measurements. A general trend in droplet microfluidics is reducing the size of the droplets, but the challenge is maintaining the particles in the droplets after splitting. We have previously reported on an acoustofluidic chip where bulk acoustic waves were used to control particle positioning in a trident-shaped droplet split. However, the reported particle enrichment was modest (3-fold), and the aim of this study is to increase the particle enrichment by optimising the droplet split design. With our new optimised droplet split we show up to 16.7-fold particle enrichment with high particle recovery.

  • 9.
    Fornell, Anna
    et al.
    Lund University.
    Nilsson, Johan
    Lund University.
    Jonsson, Linus
    Lund University.
    Periyannan Rajeswari, Prem
    KTH, Science for Life Laboratory.
    Joensson, Haakan
    KTH, Science for Life Laboratory.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund University.
    Particle enrichment in two-phase microfluidic systems using acoustophoresis2016Conference paper (Refereed)
  • 10.
    Fornell, Anna
    et al.
    Lund University.
    Ohlin, Mathias
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Garofalo, Fabio
    Department Biomedical Engineering, Lund University.
    Nilsson, Johan
    Department Biomedical Engineering, Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Department Biomedical Engineering, Lund University.
    An intra-droplet particle switch for droplet microfluidics using bulk acoustic waves2017In: Biomicrofluidics, ISSN 1932-1058, E-ISSN 1932-1058, Vol. 11, article id 031101Article in journal (Refereed)
    Abstract [en]

    To transfer cell- and bead-assays into droplet-based platforms typically requires the use of complex microfluidic circuits, which calls for methods to switch the direction of the encapsulated particles. We present a microfluidic chip where the combination of acoustic manipulation at two different harmonics and a trident-shaped droplet-splitter enables direction-switching of microbeads and yeast cells in droplet microfluidic circuits. At the first harmonic, the encapsulated particles exit the splitter in the center daughter droplets, while at the second harmonic, the particles exit in the side daughter droplets. This method holds promises for droplet-based assays where particle-positioning needs to be selectively controlled.

  • 11.
    Fornell, Anna
    et al.
    Department of Biomedical Engineering, Lund University, Sweden.
    Ohlin, Mathias
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Nilsson, Johan
    Department Biomedical Engineering, Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    A droplet unit operator for controlled particle switching and enrichment2017Conference paper (Refereed)
  • 12.
    Fornell, Anna
    et al.
    Department of Biomedical Engineering, Lund University, Sweden.
    Ohlin, Mathias
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Nilsson, Johan
    Department of Biomedical Engineering, Lund University, Sweden.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    An optimized droplet split designed for acoustic intra-droplet particle enrichment2017Conference paper (Refereed)
  • 13.
    Fornell, Anna
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Materials Science and Engineering, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Söderbäck, Per
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Materials Science and Engineering, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Liu, Zhenhua
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Materials Science and Engineering, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Moreira, Milena
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Materials Science and Engineering, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Materials Science and Engineering, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Fabrication of Silicon Microfluidic Chips for Acoustic Particle Focusing Using Direct Laser Writing2020In: Micromachines, ISSN 2072-666X, E-ISSN 2072-666X, Vol. 11, no 2, article id 113Article in journal (Refereed)
    Abstract [en]

    We have developed a fast and simple method for fabricating microfluidic channels in silicon using direct laser writing. The laser microfabrication process was optimised to generate microfluidic channels with vertical walls suitable for acoustic particle focusing by bulk acoustic waves. The width of the acoustic resonance channel was designed to be 380 µm, branching into a trifurcation with 127 µm wide side outlet channels. The optimised settings used to make the microfluidic channels were 50% laser radiation power, 10 kHz pulse frequency and 35 passes. With these settings, six chips could be ablated in 5 h. The microfluidic channels were sealed with a glass wafer using adhesive bonding, diced into individual chips, and a piezoelectric transducer was glued to each chip. With acoustic actuation at 2.03 MHz a half wavelength resonance mode was generated in the microfluidic channel, and polystyrene microparticles (10 µm diameter) were focused along the centre-line of the channel. The presented fabrication process is especially interesting for research purposes as it opens up for rapid prototyping of silicon-glass microfluidic chips for acoustofluidic applications.

  • 14.
    Liu, Zhenhua
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Fornell, Anna
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Barbe, Laurent
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Hjort, Klas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    On-chip background dilution in droplets with high particle recovery using acoustophoresis2019In: Biomicrofluidics, ISSN 1932-1058, E-ISSN 1932-1058, Vol. 13, article id 064123Article in journal (Refereed)
    Abstract [en]

    Droplet microfluidics has shown great potential for on-chip biological and chemical assays. However, fluid exchange in droplet microfluidics with high particle recovery is still a major bottleneck. Here, using acoustophoresis, we present for the first time a label-free method to achieve continuous background dilution in droplets containing cells with high sample recovery. The system comprises droplet generation, acoustic focusing, droplet splitting, picoinjection, and serpentine mixing on the same chip. The capacities of the picoinjection and the droplet split to dilute the background fluorescent signal in the droplets have been characterized. The sample recovery at different droplet split ratios has also been characterized. The results show a maximum of 4.3-fold background dilution with 87.7% particle recovery. We also demonstrated that the system can be used to dilute background fluorescent signal in droplets containing either polystyrene particles or endothelial cells.

  • 15.
    Liu, Zhenhua
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Fornell, Anna
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    A continuous on-chip droplet washing platform with high bead recovery by acoustofluidics2019Conference paper (Other academic)
    Abstract [en]

    Acoustofluidics is a promising technology for manipulation of fluids and particles in microchannels, and the technology has the ability to sort beads and cells in continuous flow with very high efficiency. Recently acoustofluidics has also been applied in segmental flow for positioning beads inside droplets. Compared with single-phase systems, droplet microfluidics has the advantages of faster reactions, lower cross-contamination and higher throughput. Moreover, the small size of the droplets makes them ideal as cultivation and reaction vials for single cell analysis. However, as the droplets are so small one challenge is to wash the droplets before image analysis. P. Mary et al. developed a microfluidic platform for droplet wash, whichis based on electrocoalescence and droplet break-ups with equal volume. The background noise was decreased significantly, however the recovery of the encapsulated cells was low. Alternative solutions have been presented by H. Lee et al. and S.R. Doonan et al. but as the bead recovery is controlled via magnetophoresis, the technology is only applicable to magnetic samples. Here we present a droplet microfluidic platform that enables background dilution with high bead recovery in a label-free manner using acoustophoresis.

  • 16.
    Liu, Zhenhua
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Fornell, Anna
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Droplet Dilution Unit Operation Including Bead Washing Using Integrated Acoustophoresis2019Conference paper (Other academic)
    Abstract [en]

    This paper presents a microfluidic platform for on-chip droplet dilution where the bead recovery also can be controlled. The droplets containing 10 µm polystyrene beads can be diluted with high bead recovery. This platform involves 5 steps for on-chip dilution of the droplets: droplet generation, bead focusing, droplet splitting, pico-injection and serpentine mixing. Background signal in the droplets is significantly reduced with maintained bead recovery by this on-chip dilution method. The technology is applicable to many types of samples and does not require any labelling of the bioparticles.

  • 17.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Lund University, Lund, Sweden.
    Bruus, Henrik
    Tech Univ Denmark, Lyngby, Denmark.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund University, Lund, Sweden.
    Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis2017In: Journal of Micromechanics and Microengineering, ISSN 0960-1317, E-ISSN 1361-6439, Vol. 27, no 8, article id 084002Article in journal (Refereed)
    Abstract [en]

    We have fabricated a silicon-glass two-phase droplet microfluidic system capable of generating sub 100 µm-sized,   =  (74  ±  2) µm, spherical droplets at rates of up to hundreds of hertz. By implementing a two-dimensional (2D) acoustophoresis particle-positioning method, we show a fourfold improvement in both vertical and lateral particle positioning inside the droplets compared to unactuated operation. The efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37 °C). Furthermore, by using acoustic actuation, (99.8  ±  0.4)% of all encapsulated microparticles can be detected compared to only (79.0  ±  5.1)% for unactuated operation. In our experiments we observed a strong ordering of the microparticles in distinct patterns within the droplet when using 2D acoustophoresis; to explain the origin of these patterns we simulated numerically the fluid flow inside the droplets and compared with the experimental findings.

  • 18.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Department of Biomedical Engineering, Lund University, Sweden.
    Bruus, Henrik
    Department of Physics, Technical University of Denmark, Kgs. Lyngby, Denmark.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Using two-dimensional acoustophoresis for improved particle positioning in droplet microfluidics2017Conference paper (Refereed)
  • 19.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Department of Biomedical Engineering, Lund University, Sweden .
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Department of Biomedical Engineering, Lund University, Sweden .
    2D acoustophoretic positioning of microparticles inside droplets2016Conference paper (Refereed)
  • 20.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Department of Biomedical Engineering, Lund University, Sweden .
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Department of Biomedical Engineering, Lund University, Sweden .
    Two-dimensional acoustic focusing of microparticles in two-phase droplet-based microfluidic systems for improved particle positioning within spherical droplets2016Conference paper (Refereed)
    Abstract [en]

    We have fabricated a silicon-glass microfluidic two-phase droplet generator capable of generating sub 100-micrometer-sized (⌀ ൌ74 μm ± 2 μm) spherical droplets at rates up to hundreds of hertz (298 Hz ± 85 Hz). Furthermore, we have implemented a two-dimensional acoustic focusing technique into the device. Here, we show that applying the focusing to 10 μm sized polystyrene particles during the droplet generation step, results in a fourfold improvement of the particle positioning (centricity) within the generated droplets compared to the unactuated control. Finally, the efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37°C).

  • 21.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund University.
    Two-dimensional acoustic focusing of microparticles in two-phase droplet-based microfluidic systems increases particle detectability2016Conference paper (Refereed)
    Abstract [en]

    We have fabricated a silicon-glass two-phase droplet-based microfluidic system and implemented two-dimensional acoustic focusing prior to droplet generation as well as continuously throughout the whole system to increase particle detectability. Using acoustic focusing we have effectivelyminimized sedimentation of the encapsulated particles and thereby increased particle detectability by as much as 44% compared to unactuated operation of the system.

1 - 21 of 21
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