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  • 1.
    Alajlani, M.
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi. Karl Franzens Univ Graz, Inst Pharmaceut Sci, Dept Pharmacognosy, Univ Pl 4, A-8010 Graz, Austria..
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi.
    Predicting the mechanism of action of antituberculosis agents using chemical global positioning system - natural product2016Ingår i: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 82Artikel i tidskrift (Övrigt vetenskapligt)
  • 2.
    Alajlani, Muaaz Mutaz
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi. Univ Halle Wittenberg, Inst Pharm, Dept Pharmaceut Biol Pharmacognosy, Hoher Weg 8, DE-06120 Halle, Saale, Germany..
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi.
    Evaluating Antimycobacterial Screening Schemes Using Chemical Global Positioning System-Natural Product Analysis2020Ingår i: Molecules, ISSN 1420-3049, E-ISSN 1420-3049, Vol. 25, nr 4, artikel-id 945Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Most of the targeted discoveries in tuberculosis research have covered previously explored chemical structures but neglected physiochemical properties. Until now, no efficient prediction tools have been developed to discriminate the novelty of screened compounds at early stages. To overcome this deficit, a drastic novel approach must include physicochemical properties filters provided by Chemical Global Positioning System-Natural Product analysis (ChemGPS-NP). Three different screening schemes GSK, GVKBio, and NIAID provided 776, 2880, and 3779 compounds respectively and were evaluated based on their physicochemical properties and thereby proposed as deduction examples. Charting the physiochemical property spaces of these sets identified the merits and demerits of each screening scheme by simply observing the distribution over the chemical property space. We found that GSK screening set was confined to a certain space, losing potentially active compounds when compared with an in-house constructed 459 highly active compounds (active set), while the GVKBio and NIAID screening schemes were evenly distributed through space. The latter two sets had the advantage, as they have covered a larger space and presented compounds with additional variety of properties and activities. The in-house active set was cross-validated with MycPermCheck and SmartsFilter to be able to identify priority compounds. The model demonstrated undiscovered spaces when matched with Maybridge drug-like space, providing further potential targets. These undiscovered spaces should be considered in any future investigations. We have included the most active compounds along with permeability and toxicity filters as supplemented material.

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  • 3.
    Alsmark, Cecilia
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Strese, Åke
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Wedén, Christina
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Microbial diversity of Alcyonium digitatum2013Ingår i: Phytochemistry Reviews, ISSN 1568-7767, E-ISSN 1572-980X, Vol. 12, nr 3, s. 531-542Artikel, forskningsöversikt (Refereegranskat)
    Abstract [en]

    Marine multi-cellular organisms are described as sources of many newly discovered bioactive compounds. Meanwhile, it has been demonstrated repeatedly for several natural products of reputed multicellular origin that they are, in fact, produced by endophytic unicellular organisms-such as microbial fungi or bacteria. Consequently, while studying compounds isolated from a living organism, it is essential to ensure that the sample integrity is not compromised. To test the diversity of the endobiome from Alcyonium digitatum, a cold water coral found along the Atlantic coasts of the northern hemisphere, we performed a culture dependent surveyed using a phylogenetic approach. A 1 cm(3) cube from the interior tissue of A. digitatum was excised under aseptic conditions, homogenized, spread onto agar-based growth medium plates and incubated in 22 A degrees C to promote microbial growth. Colonies were transferred to secondary medium plates, incubated, and after harvesting lysed using sterile water to release DNA. 16S and 23S rDNA regions were amplified using PCR, and sequenced for systematic evaluation using phylogenetic analysis. From this survey we identified a broad selection of bacteria, predominantly of the alpha-proteobacterial, bacteriodete, actinobacterial and firmicute lineages, demonstrating a significant biodiversity of the coral bacterial endobiome.

  • 4.
    Andersson, Inger
    et al.
    Department of Molecular Biology, Swedish University of Agricultural Sciences.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Structure and function of Rubisco2008Ingår i: Plant physiology and biochemistry (Paris), ISSN 0981-9428, E-ISSN 1873-2690, Vol. 46, nr 3, s. 275-291Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is the major enzyme assimilating CO2 into the biosphere. At the same time Rubisco is an extremely inefficient catalyst and its carboxylase activity is compromised by an opposing oxygenase activity involving atmospheric O2. The shortcomings of Rubisco have implications for crop yield, nitrogen and water usage, and for the global carbon cycle. Numerous high-resolution crystal structures of different forms of Rubisco are now available, including structures of mutant enzymes. This review uses the information provided in these structures in a structure-based sequence alignment and discusses Rubisco function in the context of structural variations at all levels – amino acid sequence, fold, tertiary and quaternary structure – with an evolutionary perspective and an emphasis on the structural features of the enzyme that may determine its function as a carboxylase.

  • 5.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi.
    Bioactivity mapping of chemical property space - possible applications in natural products research2016Ingår i: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 82Artikel i tidskrift (Övrigt vetenskapligt)
  • 6.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi.
    Columelliaceae2016Ingår i: The Families and Genera of Vascular Plants / [ed] K. Kubitzki, J. Kadereit, and V. Bittrich, Springer, 2016, s. 141-144Kapitel i bok, del av antologi (Refereegranskat)
  • 7.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Dipsacales (Honeysuckle)2002Ingår i: Encyclopedia of Life Sciences, John Wiley & Sons , 2002Kapitel i bok, del av antologi (Övrigt vetenskapligt)
    Abstract [en]

    The plant order Dipsacales comprises about 2000 species belonging to the eight core families Adoxaceae, Caprifoliaceae, Collumelliaceae, Diervillaceae, Dipsacaceae, Linnaeceae, Morinaceae and Valerianaceae. It includes many ornamental plants such as honeysuckle and weigela.

  • 8.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Impulse Lecture: The odd fellow - Benefits of anomaly2014Ingår i: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 80, nr 16, s. 1373-1374Artikel i tidskrift (Övrigt vetenskapligt)
  • 9.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Phylogeny and chemography2008Ingår i: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 74, nr 9, s. SL64-Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Natural compounds are evolutionary selected and pre-validated by Nature, displaying a unique diversity of chemical properties and corresponding biological activities. Of utmost importance for a rational discovery and exploration of new biologically active compounds are two aspects: one the identification and charting of the biologically relevant chemical space, the other a similar charting of the corresponding evolutionary space.

    he first key to this is the coverage of the natural products' chemical space. For this purpose we introduced ChemGPS-NP, with the aim to provide a tool for more efficient and stringent compound comparison, to identify parts of chemical space related to particular biological activities, and to track changes in chemical properties due to e.g. evolutionary traits and modifications in biosynthesis. Physical-chemical properties not directly discernible from structural data can be compared, making selection more rational when screening natural compounds and analogues.

    The second key would consequently be to explore evolutionary space by elucidating and utilising robust phylogenies for the organisms under study. From this basis reflecting the evolutionary history and hence biosynthesis development, further conclusions can be drawn.

    Based these initial attempts, the intersection of chemical and evolutionary space have been explored. With regard to e.g. compound classes such as iridoids, betalains, and sesquiterpene lactones, evolutionary patterns of changes in physical-chemical properties are observed and compared. For eight major classes of plant defence peptides analyses of structure base alignments provide arguments for rational classification. In addition, evolution of the enzyme Rubisco, have been explored with reference to major structural features.

  • 10.
    Backlund, Anders
    Uppsala universitet.
    Phylogeny of the Dipsacales1996Övrigt (Övrigt vetenskapligt)
  • 11.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Topical chemical space in relation to biological space2010Ingår i: Comprehensive Natural Products II: Chemistry and Biology / Volume 3 / [ed] L. Mander & H.-W. Lui, Oxford: Elsevier, 2010, s. 47-79Kapitel i bok, del av antologi (Refereegranskat)
    Abstract [en]

    In this chapter, the mapping of physical–chemical descriptor space of natural products and its relation to the biological space, with emphasis on evolutionary and topical biological space, is discussed. A brief presentation of methods for phylogenetic analysis and their different advantages is followed by discussions of evolutionary implications. Examples from both unpublished and previously published studies are presented.

  • 12.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi.
    Tribelaceae2016Ingår i: The Families and Genera of Vascular Plants / [ed] K. Kubitzki, J. Kadereit, and V. Bittrich, Springer, 2016, s. 377-379Kapitel i bok, del av antologi (Refereegranskat)
  • 13.
    Backlund, Anders
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Backlund, Maria
    Klum, Mattias
    Klum collection anteckningsbok2008Bok (Övrig (populärvetenskap, debatt, mm))
  • 14.
    Backlund, Anders
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi.
    Bittrich, Volker
    Adoxaceae2016Ingår i: The Families and Genera of Vascular Plants / [ed] K. Kubitzki, J. Kadereit, and V. Bittrich, Springer, 2016, s. 19-29Kapitel i bok, del av antologi (Refereegranskat)
  • 15.
    Backlund, Anders
    et al.
    Uppsala universitet.
    Bremer, Birgitta
    Uppsala universitet.
    Phylogeny of the Asteridae s. str. based on rbcL sequences, with particular reference to the Dipsacales1997Ingår i: Plant Systematics and Evolution, ISSN 0378-2697, E-ISSN 1615-6110, Vol. 207, nr 3-4, s. 225-254Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The rbcL gene of 15 taxa was sequenced and analyzed cladistically together with a large sample of genera representing all main clades of the subclass Asteridae in order to determine more precisely the delimitation of the order Dipsacales and to elucidate

  • 16.
    Backlund, Anders
    et al.
    Uppsala universitet.
    Bremer, Kåre
    Uppsala universitet.
    To be or not to be - principles of classification and monotypic plant families1998Ingår i: Taxon, ISSN 0040-0262, E-ISSN 1996-8175, Vol. 47, nr 2, s. 391-400Artikel i tidskrift (Refereegranskat)
  • 17.
    Backlund, Anders
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Buonfiglio, R.
    AstraZeneca R&D, Discovery Sci, Computat Sci, Molndal, Sweden..
    Henz, A.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Vikeved, Elisabet
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Lai, Kuei-Hung
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Kogej, T.
    AstraZeneca R&D, Discovery Sci, Computat Sci, Molndal, Sweden..
    Charting biological activity in chemical property space using ChemGPS-NP2015Ingår i: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 81, nr 16, s. 1413-1413Artikel i tidskrift (Övrigt vetenskapligt)
  • 18.
    Backlund, Anders
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Gottfries, Johan
    AstraZeneca R&D, Mölndal.
    Bohlin, Lars
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Larsson, Josefin
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Chemography and Phylogeny: navigating chemical and evolutionary space2006Ingår i: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 72, nr 11, s. 972-972Artikel i tidskrift (Övrigt vetenskapligt)
  • 19.
    Backlund, Anders
    et al.
    Uppsala universitet.
    Moritz, Thomas
    Uppsala universitet.
    Phylogenetic implications of an expanded valepotriate distribution in the Valerianaceae1998Ingår i: Biochemical Systematics and Ecology, ISSN 0305-1978, E-ISSN 1873-2925, Vol. 26, nr 3, s. 309-335Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A total of 27 taxa from the families Adoxaceae s.lat. (including Viburnaceae and Sambucaceae), Araliaceae, Caprifoliaceae, Dipsacaceae and Valerianaceae (including Triplostegia) have been analysed using thin-layer chromatography (TLC) and combined high pe

  • 20.
    Backlund, Anders
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för evolution, genomik och systematik, Systematisk botanik.
    Nilsson, Siwert
    Naturhistoriska Riksmuséet.
    Pollen morphology and the systematic position of Triplostegia (Dipsacales)1997Ingår i: Taxon, ISSN 0040-0262, E-ISSN 1996-8175, Vol. 46, nr 1, s. 21-31Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Triplostegia comprises two species of perennial herbs from southeast Asia, T. glandulifera and T. grandiflora. The systematic position of the genus has been debated ever since it was described, and it has been placed in either Dipsacaceae or Valerianaceae, or in a family of its own Triplo-stegiaceae. Pollen of Triplostegia, investigated by light microscopy and scanning electron micro-scopy, is similar to that of both Dipsacaceae and Valerianaceae. Presence of numerous branched and bent columellae as well as an aperturem argins tructurer esemblingt he halo found in Valeria-naceae indicates a closer relationship to the Valerianaceae. A sister-group relationship between Triplostegia and the Valerianaceae is furthermore supported by other studies of molecular and morphological data. In order to maximize information content in the framework of mandatory classificational ranks, Triplostegia is best included in the family Valerianaceae, as the sole mem-ber of a subfamily Triplostegioideae.

  • 21.
    Backlund, Anders
    et al.
    Uppsala universitet.
    Pyck, Nancy
    Catholic University, Leuven.
    Diervillaceae and Linnaeaceae, two new families of caprifolioids1998Ingår i: Taxon, ISSN 0040-0262, E-ISSN 1996-8175, Vol. 47, nr 3, s. 657-661Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Two new families of caprifolioids, Diervillaceae and Linnaeaceae, are proposed. They correspond to the former subfamilies Diervilloideae and Linnaeoideae. A key to their genera and those remaining in Caprifoliaceae is provided.

  • 22.
    Backlund, Anders
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Rosén, Josefin
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Larsson, Sonny
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Teoribildning öppnar nya fält för naturproduktsforskning2008Ingår i: Läkemedelsvärlden, ISSN 1402-1927, nr 6, s. 31-Artikel i tidskrift (Övrig (populärvetenskap, debatt, mm))
  • 23.
    Bohlin, Lars
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Göransson, Ulf
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Alsmark, Cecilia
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Wedén, Christina
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Natural products in modern life science2010Ingår i: Phytochemistry Reviews, ISSN 1568-7767, E-ISSN 1572-980X, Vol. 9, nr 2, s. 279-301Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    With a realistic threat against biodiversity in rain forests and in the sea, a sustainable use of natural products is becoming more and more important. Basic research directed against different organisms in Nature could reveal unexpected insights into fundamental biological mechanisms but also new pharmaceutical or biotechnological possibilities of more immediate use. Many different strategies have been used prospecting the biodiversity of Earth in the search for novel structure-activity relationships, which has resulted in important discoveries in drug development. However, we believe that the development of multidisciplinary incentives will be necessary for a future successful exploration of Nature. With this aim, one way would be a modernization and renewal of a venerable proven interdisciplinary science, Pharmacognosy, which represents an integrated way of studying biological systems. This has been demonstrated based on an explanatory model where the different parts of the model are explained by our ongoing research. Anti-inflammatory natural products have been discovered based on ethnopharmacological observations, marine sponges in cold water have resulted in substances with ecological impact, combinatory strategy of ecology and chemistry has revealed new insights into the biodiversity of fungi, in depth studies of cyclic peptides (cyclotides) has created new possibilities for engineering of bioactive peptides, development of new strategies using phylogeny and chemography has resulted in new possibilities for navigating chemical and biological space, and using bioinformatic tools for understanding of lateral gene transfer could provide potential drug targets. A multidisciplinary subject like Pharmacognosy, one of several scientific disciplines bridging biology and chemistry with medicine, has a strategic position for studies of complex scientific questions based on observations in Nature. Furthermore, natural product research based on intriguing scientific questions in Nature can be of value to increase the attraction for young students in modern life science.

  • 24.
    Bohlin, Lars
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Göransson, Ulf
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Modern pharmacognosy: Connecting biology and chemistry2007Ingår i: Pure and Applied Chemistry, ISSN 0033-4545, E-ISSN 1365-3075, Vol. 79, nr 4, s. 763-774Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    In many countries today, the number of students selecting chemistry for higher studies is decreasing. At the same time, interest in the environmental aspects of chemistry, green chemistry, and sustainable use of natural products is increasing among the young generation of students. By modernizing and renewing a venerable proven science, pharmacognosy would have a strategic position to connect biology and chemistry. This multidisciplinary subject is important for discovery of novel and unique molecules with drug potential, and for revealing unknown targets, by studying evolutionary structure-activity optimization in nature. In this paper, the overall aim and strategies of our research are presented and exemplified by three different research projects.

    Natural products are involved in scientific issues important for a sustainable society, and a multidisciplinary subject such as pharmacognosy can, therefore, be useful in increasing future interest in both chemistry and biology.

  • 25.
    Bremer, Kåre
    et al.
    Uppsala universitet.
    Chase, Mark W.
    Stevens, Peter F.
    Anderberg, Arne A.
    Backlund, Anders
    Uppsala universitet.
    Bremer, Birgitta
    Uppsala universitet.
    Briggs, B. G.
    Endress, Peter K.
    Fay, Michael F.
    Goldblatt, Peter
    Gustafsson, Mat H. G.
    Uppsala universitet.
    Hoot, S. B.
    Judd, Walter S.
    Kallersjö, Mari
    Kellogg, Elizabeth A.
    Kron, Kathleen A.
    Les, D. H.
    Morton, C. M.
    Nickrent, Daniel L.
    Olmstead, Richard G.
    Price, RA
    Quinn, C. J.
    Rodman, J. E.
    An ordinal classification for the families of flowering plants1998Ingår i: ANNALS OF THE MISSOURI BOTANICAL GARDEN, ISSN 0026-6493, Vol. 85, nr 4, s. 531-553Artikel i tidskrift (Övrigt vetenskapligt)
    Abstract [en]

    Recent cladistic analyses are revealing the phylogeny of flowering plants in increasing detail, and there is support for the monophyly of many major groups above the family level. With many elements of the major branching sequence of phylogeny established

  • 26.
    Buonfiglio, Rosa
    et al.
    AstraZeneca R&D, Chem Innovat Ctr, Discovery Sci, SE-43183 Molndal, Sweden..
    Engkvist, Ola
    AstraZeneca R&D, Chem Innovat Ctr, Discovery Sci, SE-43183 Molndal, Sweden..
    Varkonyi, Peter
    AstraZeneca R&D, Chem Innovat Ctr, Discovery Sci, SE-43183 Molndal, Sweden..
    Henz, Astrid
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Vikeved, Elisabet
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Kogej, Thierry
    AstraZeneca R&D, Chem Innovat Ctr, Discovery Sci, SE-43183 Molndal, Sweden..
    Investigating Pharmacological Similarity by Charting Chemical Space2015Ingår i: Journal of Chemical Information and Modeling, ISSN 1549-9596, E-ISSN 1549-960X, Vol. 55, nr 11, s. 2375-2390Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    In this study, biologically relevant areas of the chemical space were analyzed using ChemGPS-NP. This application enables comparing groups of ligands within a multidimensional space based on principle components derived from physicochemical descriptors. Also, 3D visualization of the ChemGPS-NP global map can be used to conveniently evaluate bioactive compound similarity and visually distinguish between different types or groups of compounds. To further establish ChemGPS-NP as a method to accurately represent the chemical space, a comparison with structure-based fingerprint has been performed. Interesting complementarities between the two descriptions of molecules were observed. It has been shown that the accuracy of describing molecules with physicochemical descriptors like in ChemGPS-NP is similar to the accuracy of structural fingerprints in retrieving bioactive molecules. Lastly, pharmacological similarity of structurally diverse compounds has been investigated in ChemGPS-NP space. These results further strengthen the case of using ChemGPS-NP as a tool to explore and visualize chemical space.

  • 27. Decraene, Louis-Phillip Ronse
    et al.
    Roels, Peter
    Smets, Erik F.
    Backlund, Anders
    Uppsala universitet.
    The floral development and floral anatomy of Chrysosplenium alternifolium, an unusual member of the Saxifragaceae1998Ingår i: Journal of plant research, ISSN 0918-9440, E-ISSN 1618-0860, Vol. 111, nr 1104, s. 573-580Artikel i tidskrift (Övrigt vetenskapligt)
    Abstract [en]

    The floral development and anatomy of Chrysosplenium alternifolium were studied with the scanning electron microscope and light microscope to understand the initiation sequence of the floral organs and the morphology of the flower, and to find suitable fl

  • 28.
    Ekenäs, Catarina
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för evolutionsbiologi, Systematisk botanik.
    Rosén, Josefin
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Wagner, Steffen
    Albert-Ludwigs-Universität, Freiburg, Germany.
    Merfort, Irmgard
    Albert-Ludwigs-Universität, Freiburg, Germany.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Andreasen, Katarina
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för evolutionsbiologi, Systematisk botanik.
    Secondary chemistry and ribosomal DNA data congruencies in Arnica (Asteraceae)2009Ingår i: Cladistics, ISSN 0748-3007, E-ISSN 1096-0031, Vol. 25, nr 1, s. 78-92Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    To investigate possible congruencies between DNA sequence data and secondary chemistry, we compared nuclear ribosomal DNA (nrDNA) sequence data, sesquiterpene lactone (STL) contents, and cytometric data from 35 accessions of 16 Arnica (Asteraceae) species and two outgroup taxa (Layia hieracioides and Madia sativa), using phylogenetic inference and principal component analysis (PCA). Several groups supporting multiple accessions of the same species (of A. montana, A. longifolia, A. gracilis, and A. chamissonis) are congruent between the phylogenetic trees based on nrDNA and STL data. Sesquiterpene lactone profiles were found to be highly consistent within multiple samples of A. montana and A. longifolia respectively. Moreover, sesquiterpene lactone data support subspecies classifications of A. chamissonis and A. parryi, with additional support from DNA sequence data and cytometric data. Morphology, STL data (PCA), cytometric data and DNA sequence data suggest a hybrid origin of one accession (A. gracilis × longifolia). In A. gracilis, A. latifolia, and Layia hieracioides, previously not investigated for STLs, we found large amounts of xanthalongin derivatives. This is the first time STLs have been reported from subtribe Madiinae.

  • 29.
    Ekenäs, Catarina
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för evolution, genomik och systematik.
    Zebrowska, Anna
    Schuler, Barbara
    Vrede, Tobias
    Andreasen, Katarina
    Backlund, Anders
    Merfort, Irmgard
    Bohlin, Lars
    Anti-inflammatory activity of extracts from Arnica (Asteraceae) species assessed by inhibition of NF-κB and release of human neutrophil elastaseIngår i: Artikel i tidskrift (Refereegranskat)
  • 30.
    Ekenäs, Catarina
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för evolution, genomik och systematik, Systematisk biologi.
    Zebrowska, Anna
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Schuler, Barbara
    Vrede, Tobias
    Department of Ecology and Environmental Science, Umeå University, Sweden .
    Andreasen, Katarina
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för evolution, genomik och systematik, Systematisk biologi.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Merfort, Irmgard
    Albert-Ludwigs- Universität, Freiburg, Germany.
    Bohlin, Lars
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Screening for Anti-Inflammatory Activity of 12 Arnica (Asteraceae) Species Assessed by Inhibition of NF-κB and Release of Human Neutrophil Elastase2008Ingår i: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 74, nr 15, s. 1789-1794Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Several species in the genus Arnica have been used in traditional medicine to treat inflammatory-related disorders. Extracts of twelve Arnica species and two species closely related to Arnica (Layia hieracioides and Madia sativa) were investigated for inhibition of human neutrophil elastase release and inhibition of transcription factor NF-κB. Statistical analyses reveal significant differences in inhibitory capacities between extracts. Sesquiterpene lactones of the helenanolide type, of which some are known inhibitors of human neutrophil elastase release and NF-κB, are present in large amounts in the very active extracts of A. montana and A. chamissonis. Furthermore, A. longifolia, which has previously not been investigated, shows a high activity similar to that of A. montana and A. chamissonis in both bioassays. Sesquiterpene lactones of the xanthalongin type are present in large amounts in A. longifolia and other active extracts and would be interesting to evaluate further.

  • 31.
    El-Seedi, Hesham R.
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Larsson, Sonny
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Chemosystematic value of cyclopeptide alkaloids from Heisteria nitida (Olacaceae)2005Ingår i: Biochemical Systematics and Ecology, ISSN 0305-1978, E-ISSN 1873-2925, Vol. 33, nr 8, s. 831-839Artikel i tidskrift (Refereegranskat)
  • 32. Frederick, Raphael
    et al.
    Bruyere, Celine
    Vancraeynest, Christelle
    Reniers, Jeremy
    Meinguet, Celine
    Pochet, Lionel
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Masereel, Bernard
    Kiss, Robert
    Wouters, Johan
    Novel Trisubstituted Harmine Derivatives with Original in Vitro Anticancer Activity2012Ingår i: Journal of Medicinal Chemistry, ISSN 0022-2623, E-ISSN 1520-4804, Vol. 55, nr 14, s. 6489-6501Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    To overcome the intrinsic resistance of cancer cells to apoptotic stimuli, we designed and synthesized approximately 50 novel beta-carbolines structurally related to harmine. Harmine is known for its anticancer properties and is a DYRK1A inhibitor. Of the synthesized compounds, the most active in terms of growth inhibition of five cancer cell lines are cytostatic and approximately 100 times more potent than harmine but demonstrated no DYRK1A inhibitory activity. These novel beta-carbolines display similar growth inhibitory activity in cancer cells that are sensitive and resistant to apoptotic stimuli. Using ChemGPS-NP, we found that the more active beta-carbolines are all more lipophilic and larger than the less active compounds. Lastly, on the basis of the NCI human tumor cell line anticancer drug screen and the NCI COMPARE algorithm, it appears that some of these compounds, including 5a and 5k, seem to act as protein synthesis inhibitors.

  • 33. Fucik, Helena
    et al.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Farah, Mohamed
    Building a computerized herbal substance register for imnplementation and use in the whole world health organisation international drug monitoring programme2002Ingår i: Drug information journal, ISSN 0092-8615, E-ISSN 2164-9200, Vol. 36, nr 4, s. 839-854Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The overall aim of this project is to sort and structure the plant name material gathered by Mohamed Farah, PhD, in order to update the substance register of the international database of adverse drug reactions, the International Drug Information System (INTDIS). This database is used within the World Health Organisation (WHO) International Drug Monitoring Programme, which for more than 30 years has collected information about adverse drug reactions (ADRs). In recent years, it has become necessary to store information about herbal medicinal remedies as well as allopathic drugs. In order to create a structure that allowed for easy retrieval, a set of new herbal serial numbers have been assigned, which indicate mother plant, part, and type of extract used for each herbal substance; and the register was designed to contain records of scientific, vernacular and common name synonyms, which were identified as such. The system was built in a local PC environment and was implemented into INTDIS during the fall of 2001.

  • 34.
    Gustafsson, Mats H. G.
    et al.
    Uppsala universitet.
    Backlund, Anders
    Uppsala universitet.
    Bremer, Birgitta
    Uppsala universitet.
    Phylogeny of the Asterales sensu late based on rbcL sequences with particular reference to the Goodeniaceae1996Ingår i: Plant Systematics and Evolution, ISSN 0378-2697, E-ISSN 1615-6110, Vol. 199, nr 3-4, s. 217-242Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The rbcL gene of 25 taxa was sequenced and analyzed cladistically in order to define more precisely the order Aster-ales s.l. and to reconstruct the phylogeny of Goodeniaceae. The cladistic analyses show that the Asterales comprise the families Abrophylla

  • 35.
    Henz, Astrid
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi.
    Buonfiglio, R.
    AstraZeneca R&D, Discovery Sci, Chem Innovat Ctr, Computat Chem, S-43183 Molndal, Sweden..
    Kogej, T.
    AstraZeneca R&D, Discovery Sci, Chem Innovat Ctr, Computat Chem, S-43183 Molndal, Sweden..
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi.
    Phylogenetic relationships through the lens of chemoinformatic methods2016Ingår i: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 82Artikel i tidskrift (Övrigt vetenskapligt)
  • 36.
    Henz Ryen, Astrid
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi.
    Steinmetz, Julia
    Tahir, Ammar
    Jakobsson, Per-Johan
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi.
    Urban, Ernst
    Glasl, Sabine
    Bisabolane sesquiterpenes from the leaves of Lindera benzoin reduce prostaglandin E2 formation in A549 cellsIngår i: Phytochemistry Letters, ISSN 1874-3900, E-ISSN 1876-7486Artikel i tidskrift (Övrigt vetenskapligt)
    Abstract [en]

    Phytochemical investigation of leaves from the American shrub Lindera benzoin (L.) Blume (Lauraceae) resulted in the isolation of one pure compound (1) and a diastereomeric mixture of (2 and 3). The structures of these new bisabolane sesquiterpenes were elucidated via MS and extensive NMR measurements and identified as 6-(2-hydroxy-6-methylhept-5-en-2-yl)-3-(hydroxymethyl)-4-oxocyclohex-2-en-1-yl acetate (1) and 3-(hydroxymethyl)-6-(5-(2-hydroxypropan-2-yl)-2-methyltetrahydrofuran-2-yl)-4-oxocyclohex-2-en-1-yl acetate (2 and 3). The compounds were evaluated in vitro for their anti-inflammatory activity. In cellular assays, 1-3 reduced pro-inflammatory prostaglandin E2 production in A549 cells in a dose-dependent manner.

  • 37.
    Henz Ryen, Astrid
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi.
    Kogej, Thierry
    Structural classification and scaffold diversity of sesquiterpene lactones in the angiospermsIngår i: Phytochemistry, ISSN 0031-9422, E-ISSN 1873-3700Artikel i tidskrift (Övrigt vetenskapligt)
    Abstract [en]

    Sesquiterpene lactones (STLs) present one of the largest groups of plant specialized metabolites with a wide range of biological activities. They are a valuable source for new plant derived drugs and drug leads since they contain several important chemical properties responsible for their versatile therapeutic potential.

    The aim of this study was to analyze and compare the chemical diversity of all types of STLs in different plant groups, both qualitatively and quantitatively. For this purpose, over 5,200 STLs have been compiled and their plant origin has been recorded, resulting in a comprehensive dataset comprising over 8,600 entries. An overview of skeleton classes and their distribution among plant families was given by assigning the STLs to their major classes. An extensive scaffold diversity analysis was performed based on the molecular framework of these compounds using established metrics. Furthermore, molecular diversity and similarity was assessed via 2D fingerprint and clustering analysis.

    The results highlighted significant differences in the degree of chemical diversity. It was demonstrated that the investigated plant families have tendencies to produce certain types of skeletons. The quantity and distribution of skeleton classes was determined per plant family and genus, as well as the proportions of skeleton classes to other STL producing families. Analyzing the scaffold diversity showed that they possessed specific sets of molecular frameworks with a considerable variation in their frequency of occurrence. Even if many plant families produce STLs belonging to the same skeleton class, their corresponding molecular frameworks differ. Clustering analysis confirmed the known large structural diversity and revealed similarities and differences of the compounds. The metrics employed enabled to qualitatively divide STLs into smaller groups with similar structural features, which reflected biologically and chemically different STLs and pointed out the differentiation of various plant groups, down to the taxonomic rank of the species.

    Taken together, these analyses provided a comprehensive insight into scaffold and molecular diversity of STLs. Due to the detailed taxonomic annotation, the distinct distribution of different types of STLs was captured. This dataset represents the latest detailed compilation of STLs in the angiosperms, which can be used as a basis for further chemoinformatic or chemosystematic analyses. To provide an example of potential implementations, the results were utilized in a phylogenetic exploration of these metabolites.

  • 38.
    Henz Ryen, Astrid
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi.
    Charting Angiosperm Chemistry: Evolutionary Perspective on Specialized Metabolites Reflected in Chemical Property Space2019Ingår i: Journal of Natural Products, ISSN 0163-3864, E-ISSN 1520-6025, Vol. 82, nr 4, s. 798-812Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Plants possess an outstanding chemical diversity of specialized metabolites developed to adapt to environmental niches and increase fitness. The observed diversity is hypothesized to result from various evolutionary mechanisms, such as the continuous branching off and extension of existing biosynthetic pathways or enhanced levels of catalytic promiscuity in certain enzymes. In this study, ChemGPS-NP has been employed to chart the distribution and diversity of physicochemical properties for selected types of specialized metabolites from the angiosperms. Utilizing these charts, it is analyzed how different properties of various types of specialized metabolites change in different plant groups, and the chemical diversity from the volume they occupy in chemical property space is evaluated. In this context, possible underlying evolutionary mechanisms are discussed, which could explain the observed distribution and behavior in chemical property space. Based on these studies, it is demonstrated that evolutionary processes in plant specialized metabolism and the resultant metabolic diversification are reflected in chemical property space.

  • 39.
    Johansson, Senia
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Göransson, Ulf
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Luijendijk, Teus
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Claeson, Per
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Bohlin, Lars
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    A neutrophil multitarget functional bioassay to detect anti-inflammatory natural products2002Ingår i: Journal of natural products (Print), ISSN 0163-3864, E-ISSN 1520-6025, Vol. 65, nr 1, s. 32-41Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A multitarget functional bioassay was optimized as a method for detecting substances interacting with the inflammatory process of activated neutrophil granulocytes, mainly to release elastase detected by p-nitroanilide (pNA) formation. Using this bioassay, 100 fractionated extracts of 96 plants were screened, with results presented in a manner that links recorded biological activity to phylogenetic information. The plants were selected to represent a major part of the angiosperms, with emphasis on medicinal plants, Swedish anti-inflammatory plants, and plants known to contain peptides. Of the tested extracts, 41% inhibited pNA formation more than 60%, and 3% stimulated formation. The extract of Digitalbis purpurea enhanced pNA formation, and digitoxin, the active compound, was isolated and identified. Plant extracts that exhibited potent nonselective inhibition (> 80% inhibition) were evaluated further for direct inhibition of isolated elastase and trypsin enzyme. The inhibitory effect of most tested extracts on the isolated enzyme elastase was similar to that of PAF- and fMLP-induced pNA formation. Compared to trypsin, inhibition of elastase by extracts of Rubus idaeus and Tabernaemontana dichotoma was significantly higher (80% and 99%, respectively). Inhibition of trypsin by the extract of Reseda luteola was high 97%. Orders such as Lamiales and Brassicales were shown to include a comparably high proportion of plants with inhibitory extracts.

  • 40.
    Koptina, Anna
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi. Volga State Univ Technol, Yoshkar Ola 424000, Russia..
    Strese, Åke
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Alsmark, Cecilia
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi. Natl Vet Inst SVA, Dept Virol Immunobiol & Parasitol, S-75651 Uppsala, Sweden..
    Challenges to get axenic cultures of Trichomonas spp.: A new approach in eradication of contaminants and maintenance of laboratory microbiological cultures2015Ingår i: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 118, s. 25-30Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Contamination of microbiological and cell cultures is a major problem in many scientific and clinical laboratories as well as bioproduct manufacturers worldwide. In the current study we established a rapid (9 day) method to detect and eliminate fungal and bacterial contamination in cultures of the unicellular eukaryote Trichomonas spp. The developed method combines identification of the contaminating microorganisms using PCR and sequencing of the 16/18S regions followed by phylogenetic analysis. The next step was a phylogeny-guided selection of antibiotic treatments. We then used a two-step propidium iodide-resorufin assay to test the effect of selected antibiotics. The result was a quick and worthwhile purification of trichomonad laboratory cultures. Our workflow may also be implemented to obtain new isolates of trichomonads from clinical samples if initial broad-spectrum antibiotic therapy fails.

  • 41.
    Korinek, Michal
    et al.
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan.;Kaohsiung Med Univ, Dept Biotechnol, Coll Life Sci, Kaohsiung, Taiwan..
    Tsai, Yi-Hong
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan..
    El-Shazly, Mohamed
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan.;Ain Shams Univ, Dept Pharmacognosy, Fac Pharm, Cairo, Egypt..
    Lai, Kuei-Hung
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi. Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Wu, Shou-Fang
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan.;Dev Ctr Biotechnol, Nat Resource Dev Inst Pharmaceut, New Taipei, Taiwan..
    Lai, Wan-Chun
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan..
    Wu, Tung-Ying
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan..
    Chen, Shu-Li
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan..
    Wu, Yang-Chang
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan.;Kaohsiung Med Univ, Res Ctr Nat Prod & Drug Dev, Kaohsiung, Taiwan.;Kaohsiung Med Univ Hosp, Dept Med Res, Kaohsiung, Taiwan..
    Cheng, Yuan-Bin
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan.;Kaohsiung Med Univ, Res Ctr Nat Prod & Drug Dev, Kaohsiung, Taiwan.;Kaohsiung Med Univ, Ctr Infect Dis & Canc Res, Kaohsiung, Taiwan..
    Hwang, Tsong-Long
    Chang Gung Univ, Grad Inst Nat Prod, Coll Med, Taoyuan, Taiwan.;Chang Gung Univ Sci & Technol, Res Ctr Chinese Herbal Med, Res Ctr Food & Cosmet Safety, Coll Human Ecol, Taoyuan, Taiwan.;Chang Gung Univ Sci & Technol, Coll Human Ecol, Grad Inst Hlth Ind Technol, Taoyuan, Taiwan.;Chang Gung Mem Hosp, Dept Anesthesiol, Taoyuan, Taiwan..
    Chen, Bing-Hung
    Kaohsiung Med Univ, Dept Biotechnol, Coll Life Sci, Kaohsiung, Taiwan.;Kaohsiung Med Univ Hosp, Dept Med Res, Kaohsiung, Taiwan.;Natl Sun Yat Sen Univ, Inst Biomed Sci, Kaohsiung, Taiwan..
    Chang, Fang-Rong
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan.;Kaohsiung Med Univ, Ctr Infect Dis & Canc Res, Kaohsiung, Taiwan.;Natl Sun Yat Sen Univ, Dept Marine Biotechnol & Resources, Kaohsiung, Taiwan.;Kaohsiung Med Univ, Res Ctr Environm Med, Kaohsiung, Taiwan.;Kaohsiung Med Univ Hosp, Canc Ctr, Kaohsiung, Taiwan..
    Anti-allergic Hydroxy Fatty Acids from Typhonium blumei Explored through ChemGPS-NP2017Ingår i: Frontiers in Pharmacology, ISSN 1663-9812, E-ISSN 1663-9812, Vol. 8, artikel-id 356Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Increasing prevalence of allergic diseases with an inadequate variety of treatment drives forward search for new alternative drugs. Fatty acids, abundant in nature, are regarded as important bioactive compounds and powerful nutrients playing an important role in lipid homeostasis and inflammation. Phytochemical study on Typhonium blumei Nicolson and Sivadasan (Araceae), a folk anti-cancer and anti-inflammatory medicine, yielded four oxygenated fatty acids, 12R-hydroxyoctadec-9Z, 13E-dienoic acid methyl ester (1) and 10R-hydroxyoctadec-8E, 12Z-dienoic acid methyl ester (2), 9R-hydroxy-10E-octadecenoic acid methyl ester (3), and 12R *-hydroxy-10E-octadecenoic acid methyl ester (4). Isolated compounds were identified by spectroscopic methods along with GC-MS analysis. Isolated fatty acids together with a series of saturated, unsaturated and oxygenated fatty acids were evaluated for their anti-inflammatory and anti-allergic activities in vitro. Unsaturated (including docosahexaenoic and eicosapentaenoic acids) as well as hydroxylated unsaturated fatty acids exerted strong anti-inflammatory activity in superoxide anion generation (IC50 2.14-3.73 mu M) and elastase release (IC50 1.26-4.57 mu M) assays. On the other hand, in the anti-allergic assays, the unsaturated fatty acids were inactive, while hydroxylated fatty acids showed promising inhibitory activity in A23187-and antigen-induced degranulation assays (e.g., 9S-hydroxy-10E, 12Z-octadecadienoic acid, IC50 92.4 and 49.7 mu M, respectively). According to our results, the presence of a hydroxy group in the long chain did not influence the potent anti-inflammatory activity of free unsaturated acids. Nevertheless, hydroxylation of fatty acids (or their methyl esters) seems to be a key factor for the anti-allergic activity observed in the current study. Moreover, ChemGPS-NP was explored to predict the structure-activity relationship of fatty acids. The anti-allergic fatty acids formed different cluster distant from clinically used drugs. The bioactivity of T. blumei, which is historically utilized in folk medicine, might be related to the content of fatty acids and their metabolites.

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  • 42.
    Lai, K. H.
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi. Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
    Lu, M. C.
    Natl Dong Hwa Univ, Grad Inst Marine Biotechnol, Pingtung 944, Taiwan.;Natl Museum Marine Biol & Aquarium, Pingtung 944, Taiwan..
    Chang, F. R.
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung 807, Taiwan.;Kaohsiung Med Univ Hosp, Ctr Canc, Kaohsiung 80708, Taiwan.;Kaohsiung Med Univ, Res Ctr Nat Prod & New Drug, Kaohsiung 80708, Taiwan..
    Su, J. H.
    Natl Dong Hwa Univ, Grad Inst Marine Biotechnol, Pingtung 944, Taiwan.;Natl Museum Marine Biol & Aquarium, Pingtung 944, Taiwan..
    El-Shazly, M.
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung 807, Taiwan.;Ain Shams Univ, Fac Pharm, Dept Pharmacognosy & Nat Prod Chem, Org African Unity St, Cairo 11566, Egypt..
    Du, Y. C.
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung 807, Taiwan..
    Wu, T. Y.
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung 807, Taiwan..
    Hsu, Y. M.
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung 807, Taiwan..
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Farmakognosi.
    Wu, Y. C.
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung 807, Taiwan.;China Med Univ, Sch Pharm, Coll Pharm, Taichung 40402, Taiwan.;China Med Univ Hosp, Chinese Med Res & Dev Ctr, Taichung 40447, Taiwan.;China Med Univ Hosp, Ctr Mol Med, Taichung 40447, Taiwan..
    Antileukemic sesterterpenoids from a marine sponge, Luffariella sp.2016Ingår i: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 82Artikel i tidskrift (Övrigt vetenskapligt)
  • 43.
    Lai, Kuei-Hung
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi. Kaohsiung Med Univ, Coll Pharm, Grad Inst Nat Prod, Kaohsiung, Taiwan..
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Lu, M. C.
    Natl Dong Hwa Univ, Grad Inst Marine Biotechnol, Pingtung, Taiwan.;Natl Museum Marine Biol & Aquarium, Pingtung, Taiwan..
    Du, Y. C.
    Kaohsiung Med Univ, Coll Pharm, Grad Inst Nat Prod, Kaohsiung, Taiwan..
    El-Shazly, M.
    Kaohsiung Med Univ, Coll Pharm, Grad Inst Nat Prod, Kaohsiung, Taiwan.;Ain Shams Univ, Fac Pharm, Dept Pharmacognosy & Nat Prod Chem, Cairo, Egypt..
    Wu, T. Y.
    Kaohsiung Med Univ, Coll Pharm, Grad Inst Nat Prod, Kaohsiung, Taiwan..
    Hsu, Y. M.
    Kaohsiung Med Univ, Coll Pharm, Grad Inst Nat Prod, Kaohsiung, Taiwan..
    Henz, A.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Chang, F. R.
    Kaohsiung Med Univ, Coll Pharm, Grad Inst Nat Prod, Kaohsiung, Taiwan.;Kaohsiung Med Univ Hosp, Canc Ctr, Kaohsiung, Taiwan.;Kaohsiung Med Univ, Res Ctr Nat Prod & New Drug, Kaohsiung, Taiwan.;Natl Sun Yat Sen Univ, Dept Marine Biotechnol & Resources, Kaohsiung 80424, Taiwan..
    Wu, Y. C.
    Kaohsiung Med Univ, Coll Pharm, Grad Inst Nat Prod, Kaohsiung, Taiwan.;China Med Univ, Coll Pharm, Sch Pharm, Taichung, Taiwan.;China Med Univ Hosp, Chinese Med Res & Dev Ctr, Taichung, Taiwan.;China Med Univ Hosp, Ctr Mol Med, Taichung, Taiwan..
    Antileukemic lanostanoids from Poria cocos2015Ingår i: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 81, nr 16, s. 1418-1418Artikel i tidskrift (Övrigt vetenskapligt)
  • 44.
    Lai, Kuei-Hung
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi. Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan..
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Lu, M. C.
    Natl Dong Hwa Univ, Grad Inst Marine Biotechnol, Pingtung, Taiwan.;Natl Museum Marine Biol Aquarium, Pingtung, Taiwan..
    Du, Y. C.
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan..
    El-Shazly, M.
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan.;Ain Shams Univ, Fac Pharm, Dept Pharmacognosy & Nat Prod Chem, Cairo, Egypt..
    Wu, T. Y.
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan..
    Hsu, Y. M.
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan..
    Henz, A.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Chang, F. R.
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan.;Kaohsiung Med Univ Hosp, Ctr Canc, Kaohsiung, Taiwan.;Kaohsiung Med Univ, Res Ctr Nat Prod & New Drug, Kaohsiung, Taiwan.;Natl Sun Yat Sen Univ, Dept Marine Biotechnol & Resources, Kaohsiung 80424, Taiwan..
    Wu, Y. C.
    Kaohsiung Med Univ, Grad Inst Nat Prod, Coll Pharm, Kaohsiung, Taiwan.;China Med Univ, Sch Pharm, Coll Pharm, Taichung, Taiwan.;China Med Univ Hosp, Chinese Med Res & Dev Ctr, Taichung, Taiwan.;China Med Univ Hosp, Ctr Mol Med, Taichung, Taiwan..
    Antileukemic lanostanoids from Poria cocos2015Ingår i: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 81, nr 16, s. 1453-1453Artikel i tidskrift (Övrigt vetenskapligt)
  • 45.
    Lai, Kuei-Hung
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Lu, Mei-Chin
    Du, Ying-Chi
    El-Shazly, Mohamed
    Wu, Tung-Ying
    Hsu, Yu-Ming
    Henz, Astrid
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Yang, Juan-Cheng
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Chang, Fang-Rong
    Wu, Yang-Chang
    Cytotoxic Lanostanoids from Poria cocos2016Ingår i: Journal of natural products (Print), ISSN 0163-3864, E-ISSN 1520-6025, Vol. 79, nr 11, s. 2805-2813Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Six new and 16 known lanostanoids were isolated from the sclerotia of Poria cocos. The structures of the new isolates were elucidated to be 16α-hydroxy-3-oxo-24-methyllanosta-5,7,9(11),24(31)-tetraen-21-oic acid (1), 3β,16α,29-trihydroxy-24-methyllanosta-7,9(11),24(31)-trien-21-oic acid (2), 3β,16α,30-trihydroxy-24-methyllanosta-7,9(11),24(31)-trien-21-oic acid (3), 3β-acetoxy-16α,24β-dihydroxylanosta-7,9(11),25-trien-21-oic acid (4), 3β,16α-dihydroxy-7-oxo-24-methyllanosta-8,24(31)-dien-21-oic acid (5), and 3α,16α-dihydroxy-7-oxo-24-methyllanosta-8,24(31)-dien-21-oic acid (6), based on extensive spectroscopic analyses. The absolute configuration of 4 was determined using Mosher's method. The antiproliferative activity of the isolated compounds (except 3 and 4) was evaluated against four leukemic cell lines (Molt 4, CCRF-CEM, HL 60, and K562). Dehydropachymic acid (9), dehydroeburicoic acid (12), pachymic acid (14), and lanosta-7,9(11),24-trien-21-oic acid (20) exhibited an antiproliferative effect on the CCRF-CEM cancer cell line with IC50 values of 2.7, 6.3, 4.9, and 13.1 μM, respectively. Both dehydropachymic acid (9) and dehydroeburicoic acid (12) showed antiproliferative effects against Molt 4 (IC50 13.8 and 14.3 μM) and HL 60 (IC50 7.3 and 6.0 μM) leukemic cell lines. Primary computational analysis using a chemical global positioning system for natural products (ChemGPS-NP) on the active lanostanoids from P. cocos suggested that targets other than topoisomerases may be involved in the antiproliferative activity.

  • 46.
    Larsson, Josefin
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Gottfries, Johan
    Bohlin, Lars
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Expanding the ChemGPS chemical space with natural products2005Ingår i: Journal of natural products (Print), ISSN 0163-3864, E-ISSN 1520-6025, Vol. 68, nr 7, s. 985-991Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Recently various attempts have been made to increase the efficacy and precision of chemical libraries used in high-throughput screening (HTS) drug discovery approaches. One such approach is ChemGPS, which provides a defined chemical space for prescreening evaluation of chemical compound properties or virtual dereplication. In the present study, ChemGPS has been applied to a set of natural products shown to exhibit cyclooxygenase-1 and/or -2 (COX-1/2) inhibition. With the purpose of defining chemical properties and linking these to the observed mode of enzyme inhibition, this resulted in two lines of reasoning. On one hand several specific features of these compounds have been identified and discussed. Overall COX inhibition was frequently correlated with the presence of at least one ring in the structure, fragments exhibiting structural rigidity, and a relatively large molecular size. The concept "size" includes several parameters, e.g., molecular volume, weight, and number of bonds. On the other hand, and possibly even more important, was the unexpected finding that the natural products studied to a large extent fell outside the defined ChemGPS chemical space. Therefore, we also propose an expanded space for natural products: ChemGPS-NP.

  • 47.
    Larsson, Josefin
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Gottfries, Johan
    Muresan, Sorel
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    ChemGPS-NP: tuned for navigation in biologically relevant chemical space2007Ingår i: Journal of natural products (Print), ISSN 0163-3864, E-ISSN 1520-6025, Vol. 70, nr 5, s. 789-794Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Natural compounds are evolutionary selected and prevalidated by Nature, displaying a unique chemical diversity and a corresponding diversity of biological activities. These features make them highly interesting for studies of chemical biology, and in the pharmaceutical industry for development of new leads. Of utmost importance, for the discovery of new biologically active compounds, is the identification and charting of the corresponding biologically relevant chemical space. The primary key to this is the coverage of the natural products' chemical space. Here we introduce ChemGPS-NP, a new tool tuned for handling the chemical diversity encountered in natural products research, in contrast to previous tools focused on the much more restricted drug-like chemical space. The aim is to provide a framework for making compound classification and comparison more efficient and stringent, to identify volumes of chemical space related to particular biological activities, and to track changes in chemical properties due to, for example, evolutionary traits and modifications in biosynthesis. Physical-chemical properties not directly discernible from structural data can be discovered, making selection more efficient and increasing the probability of hit generation when screening natural compounds and analogues.

  • 48.
    Larsson, Sonny
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Delineating small plant polypeptides: with a proposal on their classification and circumscription2012Artikel i tidskrift (Refereegranskat)
  • 49. Larsson, Sonny
    et al.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Regarding the putative identity of a moth (Thaumetopoea spp.) allergen2009Ingår i: Allergy. European Journal of Allergy and Clinical Immunology, ISSN 0105-4538, E-ISSN 1398-9995, Vol. 64, nr 3, s. 493-493Artikel i tidskrift (Refereegranskat)
  • 50.
    Larsson, Sonny
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Backlund, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Bohlin, Lars
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
    Reappraising a decade old explanatory model for pharmacognosy2008Ingår i: Phytochemistry Letters, ISSN 1874-3900, E-ISSN 1876-7486, Vol. 1, nr 3, s. 131-134Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    It has been a decade since a tripod model of pharmacognosy - organism, biological activity, chemical structure - was proposed. Since then advances in all disciplines have taken science into the 21st century. What are the implications for pharmacognosy? In this paper we expand the previous model, adding a new module with focus on informatics to encompass results of new technical and theoretical advancements for drug discovery.

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