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  • 1. Alm, Johan S
    et al.
    Swartz, Jackie
    Björkstén, Bengt
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Engström, Johan
    Kühn, Inger
    Lilja, Gunnar
    Möllby, Roland
    Norin, Elisabeth
    Pershagen, Göran
    Reinders, Claudia
    Wreiber, Karin
    Scheynius, Annika
    An anthroposophic lifestyle and intestinal microflora in infancy.2002In: Pediatr Allergy Immunol, ISSN 0905-6157, Vol. 13, no 6, p. 402-11Article in journal (Other scientific)
  • 2. Aro, P
    et al.
    Ronkainen, J
    Storskrubb, T
    Bolling-Sternevald, E
    Carlsson, R
    Johansson, S E
    Vieth, M
    Stolte, M
    Engstrand, L
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Talley, N J
    Agréus, L
    Valid symptom reporting at upper endoscopy in a random sample of the Swedish adult general population: the Kalixanda study.2004In: Scand J Gastroenterol, ISSN 0036-5521, Vol. 39, no 12, p. 1280-8Article in journal (Other scientific)
  • 3.
    Atuma, C
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology, Integrative Physiology.
    Engstrand, Lars
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Physiology.
    Holm, Lena
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Physiology.
    Helicobacter pylori extracts reduce gastric mucosal blood flow by a nitric oxide-independent but mast cell- and platelet-activating factor receptor-dependent pathway in rats1999In: Scandinavian Journal of Gastroenterology, ISSN 0036-5521, E-ISSN 1502-7708, Vol. 34, no 12, p. 1183-1189Article in journal (Refereed)
    Abstract [en]

    BACKGROUND: We have previously shown that water extracts from Helicobacter pylori reduce gastric mucosal blood flow by approximately 15%. It has also been suggested that H. pylori can inhibit endogenous nitric oxide (NO) biosynthesis. Our aim was to examine whether the reduction in blood flow induced by H. pylori is the direct consequence of an NO synthase inhibition and the possible involvement of mast cell degranulation.

    METHODS: A water extract was produced from wildtype strain 88-23. The extract was applied on the exteriorized gastric corporal mucosa in inactin-anesthetized rats, after removing as much as possible of the mucus layer, during intravital microscopy. Blood flow was measured with laser-Doppler flowmetry.

    RESULTS: In rats pretreated with the NO synthase inhibitor N-nitro-L-arginine there was a 19% +/- 6% reduction in blood flow 40 min after application of the extract, and a 27% +/- 9% reduction after another 20 min with saline. The reduction was abolished by concomitant pretreatment with the mast cell stabilizer ketotifen or the platelet-activating factor (PAF) receptor antagonist WEB2086.

    CONCLUSION: The reduction in mucosal blood flow induced by the extract was probably mediated through an acute inflammatory response involving mast cell degranulation with consequent PAF secretion. The effect on blood flow was not the result of a decrease in vascular tone due to an inhibition of endogenous NO biosynthesis.

  • 4.
    Atuma, Christer
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Physiology.
    Engstrand, Lars
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Physiology.
    Holm, Lena
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Physiology.
    Extracts of Helicobacter pylori reduce gastric mucosal blood flow through a VacA- and CagA-independent pathway in rats1998In: Scandinavian Journal of Gastroenterology, ISSN 0036-5521, E-ISSN 1502-7708, Vol. 33, no 12, p. 1256-1261Article in journal (Refereed)
    Abstract [en]

    BACKGROUND: Helicobacter pylori may interfere with gastroduodenal protective mechanisms. Such effects could be due to a direct interaction with gastric epithelial cells but also to the action of a wide range of secreted and membrane-bound virulence factors. Our aim was to study the acute effects of water extracts produced from H. pylori on gastric mucosal blood flow and acid secretion and to relate them to VacA and CagA activity.

    METHOD: Extracts were produced from strains 88-23 and A5, both wild type; A5VacA, an isogenic mutant lacking expression of the vacuolating cytotoxin (VacA) and the immunodominant antigen (CagA); and Escherichia coli strain ATCC-25922. Bacterial extracts were applied on the exteriorized gastric corporal mucosa in inactin-anaesthetized rats after removal of as much as possible of the mucus layer, during intravital microscopy. Blood flow was measured by means of laser-Doppler flowmetry.

    RESULTS: All H. pylori extracts, including the extract from 88-23 heated to 100 degrees C for 30 min, significantly reduced blood flow by 15%-19%, whereas E. coli had no significant effect on blood flow.

    CONCLUSION: A factor or a combination of factors, other than VacA and CagA released from H. pylori, might compromise the natural defence of the gastric corporal mucosa by reducing mucosal blood flow. The factor is heat-stable and lacking or less potent in E. coli.

  • 5. Björkholm, B
    et al.
    Falk, P
    Engstrand, L
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Nyrén, O
    Helicobacter pylori: resurrection of the cancer link.2003In: J Intern Med, ISSN 0954-6820, Vol. 253, no 2, p. 102-19Article in journal (Other scientific)
  • 6. Björkholm, B
    et al.
    Lundin, A
    Sillén, A
    Guillemin, K
    Salama, N
    Rubio, C
    Gordon, J I
    Falk, P
    Engstrand, L
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Comparison of genetic divergence and fitness between two subclones of Helicobacter pylori.2001In: Infect Immun, ISSN 0019-9567, Vol. 69, no 12, p. 7832-8Article in journal (Other scientific)
  • 7. Björkholm, Britta
    et al.
    Guruge, Janaki
    Karlsson, Maria
    O'Donnell, David
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Falk, Per
    Gordon, Jeffrey
    Gnotobiotic transgenic mice reveal that transmission of Helicobacter pylori is facilitated by loss of acid-producing parietal cells in donors and recipients.2004In: Microbes Infect, ISSN 1286-4579, Vol. 6, no 2, p. 213-20Article in journal (Other scientific)
  • 8. Björkholm, Britta M
    et al.
    Guruge, Janaki L
    Oh, Jung D
    Syder, Andrew J
    Salama, Nina
    Guillemin, Karen
    Falkow, Stanley
    Nilsson, Christina
    Falk, Per G
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Gordon, Jeffrey I
    Colonization of germ-free transgenic mice with genotyped Helicobacter pylori strains from a case-control study of gastric cancer reveals a correlation between host responses and HsdS components of type I restriction-modification systems.2002In: J Biol Chem, ISSN 0021-9258, Vol. 277, no 37, p. 34191-7Article in journal (Other scientific)
  • 9. Blomstergren, Anna
    et al.
    Lundin, Annelie
    Nilsson, Christina
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Lundeberg, Joakim
    Comparative analysis of the complete cag pathogenicity island sequence in four Helicobacter pylori isolates.2004In: Gene, ISSN 0378-1119, Vol. 328, p. 85-93Article in journal (Other scientific)
  • 10. Carlsson, S.
    et al.
    Wiklund, N. P.
    Engstrand, Lars
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
    Weitzberg, E.
    Lundberg, J. O. N.
    Effects of pH, nitrite, and ascorbic acid on nonenzymatic nitric oxide generation and bacterial growth in urine2001In: Nitric oxide, ISSN 1089-8603, E-ISSN 1089-8611, Vol. 5, no 6, p. 580-586Article in journal (Other academic)
    Abstract [en]

    Nitrite may be generated by bacteria in urine during urinary tract infections. Acidification of nitrite results in the formation of nitric oxide (NO) and other reactive nitrogen oxides, which are toxic to a variety of microorganisms. We have studied NO formation and bacterial growth in mildly acidified human urine containing nitrite and the reducing agent vitamin C. Urine collected from healthy subjects was incubated in closed syringes at different pH values with varying amounts of nitrite and/or ascorbic acid added. NO generation was measured in headspace gas using a chemiluminescence technique. A similar setup was also used to study the growth of three strains of bacteria in urine. Mildly acidified nitrite-containing urine generated large amounts of NO and this production was greatly potentiated by ascorbic acid. The growth of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus saprophyticus was markedly reduced by the addition of nitrite to acidified urine. This inhibition was enhanced by ascorbic acid. In conclusion, we show that the growth of three common urinary pathogens is markedly inhibited in mildly acidified urine when nitrite is present. The bacteriostatic effect of acidified nitrite is likely related to the release of NO and other toxic reactive nitrogen intermediates. These results may help to explain the well-known beneficial effects of urinary acidification with, e.g., vitamin C in treatment and prevention of urinary tract infection.

  • 11. Daugule, I
    et al.
    Rumba, I
    Engstrand, L
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Ejderhamn, J
    Infection with cagA-positive and cagA-negative types of Helicobacter pylori among children and adolescents with gastrointestinal symptoms in Latvia.2003In: Eur J Clin Microbiol Infect Dis, ISSN 0934-9723, Vol. 22, no 10, p. 622-4Article in journal (Other scientific)
  • 12.
    Engstrand, Lars
    et al.
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Enroth, Helena
    [From a controversial minor finding to the Nobel Prize. The discovery of Helicobacter pylori--an unlikely tale of success]2005In: Lakartidningen, ISSN 0023-7205, Vol. 102, no 49, p. 3779-80, 3785Article in journal (Other scientific)
  • 13. Enroth, H
    et al.
    Kraaz, W
    Rohan, T
    Nyrén, O
    Engstrand, L
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Does the method of Helicobacter pylori detection influence the association with gastric cancer risk?2002In: Scand J Gastroenterol, ISSN 0036-5521, Vol. 37, no 8, p. 884-90Article in journal (Other scientific)
  • 14.
    Fohlman, Jan
    et al.
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Blomberg, Jonas
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Froman, Gunnar
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Johansson, Anders
    Friman, Göran
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Mikrobiell diagnostik med PCR blir kliniskt värdefull när analystiden kortas [Microbial diagnosis with PCR will become clinically beneficial with a faster analysis]2004In: Läkartidningen, ISSN 0023-7205, Vol. 101, no 17, p. 1488-92Article in journal (Refereed)
  • 15.
    Gustafsson, Ingegerd
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Bacteriology.
    Sjölund, Maria
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Bacteriology.
    Torell, Erik
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Infectious Diseases.
    Johannesson, Marie
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health.
    Engstrand, Lars
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Bacteriology.
    Cars, Otto
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Bacteriology.
    Andersson, Dan I.
    Bacteria with increased mutation frequency and antibiotic resistance are enriched in the commensal flora of patients with high antibiotic usage2003In: Journal of Antimicrobial Chemotherapy, ISSN 0305-7453, E-ISSN 1460-2091, Vol. 52, no 4, p. 645-650Article in journal (Refereed)
    Abstract [en]

    BACKGROUND: We examined how prolonged antibiotic treatment affected the resistance and mutation frequency of human microflora isolated from intestine (Escherichia coli, enterococci spp.), pharynx (alpha-streptococci) and nostril (coagulase-negative staphylococci, CoNS).

    METHODS: Samples were collected from patients at the Center of Cystic Fibrosis (n=18) and the haematology ward (n=18) of the University Hospital, Uppsala, Sweden. The individually used amount of antibiotics for 1 year was recorded as the defined daily dose (DDD). Primary health care patients (n=30), with no antibiotic treatment for 1 year before sampling, were used as controls. Three isolates of each bacterium from each patient were examined. Antibiotic susceptibilities were determined by disc diffusion. Mutation frequencies to rifampicin resistance were measured on 30 independent cultures of each bacterial species from each individual by plating on rifampicin agar plates. For alpha-streptococci the mutation frequency to streptomycin resistance was also determined.

    RESULTS: Isolates from patients with high antibiotic use showed a pronounced shift towards increased resistance and a small but significant increase in the mutation frequency compared with isolates from the controls. For E. coli, enterococci and CoNS the increase in geometric mean mutation frequency in the patient group was 3-, 1.8- and 1.5-fold, respectively (P values 0.0001, 0.016 and 0.012). For alpha-streptococci there was a significant difference in geometric mean mutation frequency between patient and control groups for streptomycin resistance (P=0.024) but not for rifampicin resistance (P=0.74).

    CONCLUSIONS: High antibiotic use selected for commensals with highly increased resistance and a slight increase in mutation frequency.

  • 16. Held, Maria
    et al.
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Hansson, Lars-Erik
    Bergström, Reinhold
    Wadström, Torkel
    Nyrén, Olof
    Is the association between Helicobacter pylori and gastric cancer confined to CagA-positive strains?2004In: Helicobacter, ISSN 1083-4389, Vol. 9, no 3, p. 271-7Article in journal (Other scientific)
  • 17.
    Henriksnäs, Johanna
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
    Phillipson, Mia
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
    Petersson, Joel
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
    Engstrand, L
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
    Holm, Lena
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
    An in vivo model for gastric physiological and pathophysiological studies in the mouse2005In: Acta Physiologica Scandinavica, ISSN 0001-6772, E-ISSN 1365-201X, Vol. 184, no 2, p. 151-159Article in journal (Refereed)
    Abstract [en]

    Aim:  In vivo models for studying gastrointestinal physiology and pathophysiology are well established in rats. Since a number of genetically modified mice are available there is a need for reliable mouse models. The aim of this project was to develop an in vivo mouse model for gastrointestinal studies.

    Methods: C57bl/6, NMRI and transgenic FVB/N (expressing human α-1,3/4-fucosyltransferase) mice were anaesthetized with isoflurane and the gastric mucosa exteriorized for intravital microscopy. Acid–base status and acid secretion were measured and blood pressure was continuously monitored. Gastric mucosal blood flow was recorded by laser-Doppler flowmetry. Mucus thickness and accumulation rate were measured with micropipettes.

    Results: We have developed an in vivo mouse model for studies of the gastric mucosa. With isoflurane anaesthesia the preparation can be studied for up to 5 h with stable blood pressure and mucosal blood flow. Acid–base status agrees with results from other laboratories. Blood flow increased in both C57bl/6 and α1.3/4-FT mice in response to luminal HCl, and the mucus gel could be divided into a firmly and a loosely adherent layer, all comparable with results in the rat. However, the firmly adherent mucus layer was thinner (45 ± 2 μm), and the mucus accumulation rate lower, than in the rat. Furthermore, both basal and stimulated acid secretion showed lower outputs than in the rat.

    Conclusions: This model has great potential for investigations of gastrointestinal physiology and pathophysiology and can be applied for Helicobacter pylori infection studies.

  • 18. Hjalmarsson, Sandra
    et al.
    Alderborn, Anders
    Fock, Caroline
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Muldin, Ingrid
    Kling, Helene
    Uhlén, Mathias
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Rapid combined characterization of microorganism and host genotypes using a single technology.2004In: Helicobacter, ISSN 1083-4389, Vol. 9, no 2, p. 138-45Article in journal (Other scientific)
  • 19. Hjalmarsson, Sandra
    et al.
    Sjölund, Maria
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Determining antibiotic resistance in Helicobacter pylori.2002In: Expert Rev Mol Diagn, ISSN 1473-7159, Vol. 2, no 3, p. 267-72Article in journal (Other scientific)
  • 20. Hynes, Sean O
    et al.
    Broutet, Nathalie
    Wadström, Torkel
    Mikelsaar, Marika
    O'Toole, Paul W
    Telford, John
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Kamiya, Shigeru
    Mentis, Andreas F
    Moran, Anthony P
    Phenotypic variation of Helicobacter pylori isolates from geographically distinct regions detected by lectin typing.2002In: J Clin Microbiol, ISSN 0095-1137, Vol. 40, no 1, p. 227-32Article in journal (Other scientific)
  • 21.
    Jönsson, Maria
    et al.
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
    Qvarnström, Yvonne
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
    Engstrand, Lars
    Department of Medical Sciences.
    Swedberg, Göte
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
    Clarithromycin treatment selects for persistent macrolide-resistant bacteria in throat commensal flora.2005In: Int J Antimicrob Agents, ISSN 0924-8579, Vol. 25, no 1, p. 68-74Article in journal (Refereed)
  • 22. Kivi, M
    et al.
    Tindberg, Y
    Bengtsson, C
    Engstrand, L
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Granström, M
    Assessment of the cag pathogenicity island status of Helicobacter pylori infections with serology and PCR.2005In: Clin Microbiol Infect, ISSN 1198-743X, Vol. 11, no 1, p. 66-8Article in journal (Other scientific)
  • 23. Kivi, Mårten
    et al.
    Tindberg, Ylva
    Sörberg, Mikael
    Casswall, Thomas H
    Befrits, Ragnar
    Hellström, Per M
    Bengtsson, Carina
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Granström, Marta
    Concordance of Helicobacter pylori strains within families.2003In: J Clin Microbiol, ISSN 0095-1137, Vol. 41, no 12, p. 5604-8Article in journal (Other scientific)
  • 24. Klaamas, Kersti
    et al.
    Kurtenkov, Oleg
    Rittenhouse-Olson, Kate
    Brjalin, Vadim
    Miljukhina, Ljudmila
    Shljapnikova, Ljudmila
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Expression of tumor-associated Thomsen-Friedenreich antigen (T Ag) in Helicobacter pylori and modulation of T Ag specific immune response in infected individuals.2002In: Immunol Invest, ISSN 0882-0139, Vol. 31, no 3-4, p. 191-204Article in journal (Other scientific)
  • 25.
    Ljungdahl, Mikael
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences.
    Österberg, Johanna
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences.
    Ransjö, Ulrika
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
    Engstrand, Lars
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
    Haglund, Ulf
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences.
    Inflammatory response in patients with malignant obstructive jaundice2007In: Scandinavian Journal of Gastroenterology, ISSN 0036-5521, E-ISSN 1502-7708, Vol. 42, no 1, p. 94-102Article in journal (Refereed)
    Abstract [en]

    Objective. Surgery in patients with malignant obstructive jaundice is associated with increased risks for postoperative septic complications. The aim of this study was to investigate the inflammatory and the local cellular immune response in patients accepted for surgery because of tumours in the hepatic-pancreatic-biliary (HPB) tract. Material and methods. Patients with obstructive jaundice (group HPB+) were compared with those without (HPB-). Patients undergoing surgery for benign abdominal disorders served as controls. Obstructive jaundice was present in 18 out of 33 HPB patients. Preoperatively, blood was analysed for bacteria, endotoxins and cytokines (TNF-α, IL-6 and IL-10). At operation, mesenteric lymph nodes (MLNs) were excised for bacterial cultures using standard microbiological techniques, and immunohistochemistry, using antibodies CD4 and CD8 (mainly staining T lymphocytes), CD68 (macrophages), and anti-caspase-3 (to determine the rate of apoptosis). Results. Bacterial translocation was not demonstrated in any of the patients. Increased preoperative concentrations of endotoxins were found in group HPB+. The number of macrophages and the rate of apoptosis in MLNs were increased in jaundiced patients, while the number of T lymphocytes was decreased. Conclusions. Malignant obstructive jaundice causes increased blood concentrations of endotoxins and cytokines, an increased number of macrophages in MLNs, a higher rate of apoptosis in MLNs, but a decreased number of T lymphocytes in MLNs. The lymphocyte depletion is probably due to the increased rate of apoptosis, and might reduce the ability of jaundiced patients to eradicate infection.

  • 26. Lundin, Annelie
    et al.
    Björkholm, Britta
    Kupershmidt, Ilya
    Unemo, Magnus
    Nilsson, Peter
    Andersson, Dan I
    Swedish Institute for Infectious Disease Control, Solna, Sweden & Karolinska Institute, Stockholm.
    Engstrand, Lars
    Swedish Institute for Infectious Disease Control, Solna, Sweden & Karolinska Institute, Stockholm.
    Slow genetic divergence of Helicobacter pylori strains during long-term colonization2005In: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 73, no 8, p. 4818-4822Article in journal (Refereed)
    Abstract [en]

    The genetic variability of Helicobacter pylori is known to be high compared to that of many other bacterial species. H. pylori is adapted to the human stomach, where it persists for decades, and adaptation to each host results in every individual harboring a distinctive bacterial population. Although clonal variants may exist within such a population, all isolates are generally genetically related and thus derived from a common ancestor. We sought to determine the rate of genetic change of H. pylori over 9 years in two asymptomatic adult patients. Arbitrary primed PCR confirmed the relatedness of individual subclones within a patient. Furthermore, sequencing of 10 loci (similar to 6,000 bp) in three subclones per time and patient revealed only two base pair changes among the subclones from patient I. All sequences were identical among the patient II subclones. However, PCR amplification of the highly divergent gene amiA revealed great variation in the size of the gene between the subclones within each patient. Thus, both patients harbored a single strain with clonal variants at both times. We also studied genetic changes in culture- and mouse-passaged strains, and under both conditions no genetic divergence was found. These results suggest that previous estimates of the rate of genetic change in H. pylori within an individual might be overestimates.

  • 27. Lundin, Annelie
    et al.
    Nilsson, Christina
    Gerhard, Markus
    Andersson, Dan I
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
    Krabbe, Margareta
    Engstrand, Lars
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
    The NudA protein in the gastric pathogen Helicobacter pylori is an ubiquitous and constitutively expressed dinucleoside polyphosphate hydrolase2003In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 278, no 14, p. 12574-12578Article in journal (Other academic)
    Abstract [en]

    The gastric pathogen Helicobacter pylori harbors one Nudix hydrolase, NudA, that belongs to the nucleoside polyphosphate hydrolase subgroup. In this work, the enzymatic activity of purified recombinant NudA protein was analyzed on a number of nucleoside polyphosphates. This predicted 18.6-kDa protein preferably hydrolyzes diadenosine tetraphosphate, Ap(4)A at a k(cat) of 0.15 s(-1) and a K(m) of 80 microm, resulting in an asymmetrical cleavage of the molecule into ATP and AMP. To study the biological role of this enzyme in H. pylori, an insertion mutant was constructed. There was a 2-7-fold decrease in survival of the mutant as compared with the wild type after hydrogen peroxide exposure but no difference in survival after heat shock or in spontaneous mutation frequency. Western blot analyses revealed that NudA is constitutively expressed in H. pylori at different growth stages and during stress, which would indicate that this protein has a housekeeping function. Given that H. pylori is a diverse species and that all the H. pylori strains tested in this study harbor the nudA gene and show protein expression, we consider NudA to be an important enzyme in this bacterium.

  • 28.
    Lytsy, Birgitta
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology and Infectious Medicine, Clinical Bacteriology.
    Engstrand, Lars
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Gustafsson, Åke
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Infection medicine.
    Kaden, Rene
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology and Infectious Medicine, Clinical Bacteriology.
    Time to review the gold standard for genotyping vancomycin-resistant enterococci in epidemiology: Comparing whole-genome sequencing with PFGE and MLST in three suspected outbreaks in Sweden during 2013–20152017In: Infection, Genetics and Evolution, ISSN 1567-1348, E-ISSN 1567-7257, Vol. 54, p. 74-80Article in journal (Refereed)
    Abstract [en]

    Vancomycin-resistant enterococci (VRE) are a challenge to the health-care system regarding transmission rate and treatment of infections. VRE outbreaks have to be controlled from the first cases which means that appropriate and sensitive genotyping methods are needed.

    The aim of this study was to investigate the applicability of whole genome sequencing based analysis compared to Pulsed-Field Gel Electrophoresis (PFGE) and Multi-Locus Sequence Typing (MLST) in epidemiological investigations as well as the development of a user friendly method for daily laboratory use.

    Out of 14,000 VRE - screening samples, a total of 60 isolates positive for either vanA or vanB gene were isolated of which 38 were from patients with epidemiological links from three suspected outbreaks at Uppsala University Hospital. The isolates were genotypically characterised with PFGE, MLST, and WGS based core genome Average Nucleotide Identity analysis (cgANI). PFGE was compared to WGS and MLST regarding reliability, resolution, and applicability capacity.

    The PFGE analysis of the 38 isolates confirmed the epidemiological investigation that three outbreaks had occurred but gave an unclear picture for the largest cluster. The WGS analysis could clearly distinguish six ANI clusters for those 38 isolates.

    As result of the comparison of the investigated methods, we recommend WGS-ANI analysis for epidemiological issues with VRE. The recommended threshold for Enterococcus faecium VRE outbreak strain delineation with core genome based ANI is 98.5%.

    All referred sequences of this study are available from the NCBI BioProject number PRJNA301929.

  • 29. Nahar, Shamsun
    et al.
    Mukhopadhyay, Asish K
    Khan, Rasel
    Ahmad, Mian Mashhud
    Datta, Simanti
    Chattopadhyay, Santanu
    Dhar, Swapan Chandra
    Sarker, Shafiqul Alam
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Berg, Douglas E
    Nair, G Balakrish
    Rahman, Motiur
    Antimicrobial susceptibility of Helicobacter pylori strains isolated in Bangladesh.2004In: J Clin Microbiol, ISSN 0095-1137, Vol. 42, no 10, p. 4856-8Article in journal (Other scientific)
  • 30.
    Nilsson, Anna
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology.
    Johansson, Cecilia
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology.
    Skarp, Astrid
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology.
    Kaden, Rene
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology.
    Engstrand, Lars
    Uppsala University, Science for Life Laboratory, SciLifeLab. Department of Microbiology, Tumor and Cell Biology, Karolinska Institute.
    Rautelin, Hilpi
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology.
    Genomic and phenotypic characteristics of Swedish C. jejuni water isolates2017In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 12, article id e0189222Article in journal (Refereed)
    Abstract [en]

    Campylobacter jejuni is the most common cause of bacterial gastroenteritis. Major reservoirs are warm-blooded animals, poultry in particular, but Campylobacter can also be transmitted via water. In this paper, we have taken a closer look at the biology and potential virulence of C. jejuni water isolates. Seven C. jejuni isolates from incoming surface water at water plants in Sweden were characterized with whole genome sequencing and phenotypical testing. Multi locus sequence typing analysis revealed that these isolates belonged to groups known to include both common (ST48CC) and uncommon (ST1275CC, ST683, ST793 and ST8853) human pathogens. Further genomic characterization revealed that these isolates had potential for arsenic resistance (due to presence of arsB gene in all isolates), an anaerobic dimethyl sulfoxide oxidoreductase (in three isolates) and lacked the MarR-type transcriptional regulator gene rrpB (in all but one isolate) earlier shown to be involved in better survival under oxidative and aerobic stress. As putative virulence factors were concerned, there were differences between the water isolates in the presence of genes coding for cytolethal distending toxin (cdtABC), Type VI secretion system and sialylated LOS, as well as in biofilm formation. However, all isolates were motile and could adhere to and invade the human HT-29 colon cancer cell line in vitro and induce IL-8 secretion suggesting potential to infect humans. This is, to the best of our knowledge, the first study where C. jejuni water isolates have been characterized using whole genome sequencing and phenotypical assays. We found differences and shared traits among the isolates but also potential to infect humans.

  • 31.
    Nilsson, Anna
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology.
    Skarp, Astrid
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology.
    Johansson, Cecilia
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology.
    Kaden, Rene
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology.
    Engstrand, Lars
    Uppsala University, Science for Life Laboratory, SciLifeLab. Department of Microbiology, Tumor and Cell Biology, Karolinska Institute.
    Rautelin, Hilpi
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology.
    Characterization of Swedish Campylobacter coli clade 2 and clade 3 water isolatesIn: MicrobiologyOpen, ISSN 2045-8827, E-ISSN 2045-8827Article in journal (Refereed)
  • 32. Nilsson, Christina
    et al.
    Sillén, Anna
    Eriksson, Lena
    Strand, Mona-Lisa
    Enroth, Helena
    Normark, Staffan
    Falk, Per
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Correlation between cag pathogenicity island composition and Helicobacter pylori-associated gastroduodenal disease.2003In: Infect Immun, ISSN 0019-9567, Vol. 71, no 11, p. 6573-81Article in journal (Other scientific)
  • 33. Nilsson, Christina
    et al.
    Skoglund, Anna
    Moran, Anthony P.
    Annuk, Heidi
    Engstrand, Lars
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
    Normark, Staffan
    An enzymatic ruler modulates Lewis antigen glycosylation of Helicobacter pylori LPS during persistent infection2006In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 103, no 8, p. 2863-2868Article in journal (Other academic)
    Abstract [en]

    Helicobacter pylori persistently colonizes about half the human population and contributes to the development of peptic ulcer disease and gastric cancer. This organism has evolved means to structurally alter its surface characteristics to evade innate and adaptive immune responses. H. pylori produces LPS O-antigen units that can be posttranslationally fucosylated to generate Lewis antigens, structures also found on human epithelial cells. We demonstrate an extensive diversity of Lewis x and Lewis y expression in LPS O-antigen units, occurring over time and in different regions of the human stomach. Lewis expression patterns were correlated with the on/off status of the three fucosyltransferases (FucT), FutA, FutB, and FutC, which are regulated via slipped-strand mispairing in intragenic polyC tract regions of the corresponding genes. The alpha1,3-FucT, FutA and FutB, each contain a C-terminal heptad repeat region, consisting of a variable number of DD/NLRV/INY tandem repeats. Variations in the number of heptad repeats correlated to the sizes of O-antigen polymers to become decorated by fucose residues. Our data support a molecular ruler mechanism for how H. pylori varies its LPS fucosylation pattern, where one heptad repeat in the enzyme corresponds to one N-acetyl-beta-lactosamine unit in the O-antigen polysaccharide.

  • 34. Oh, Jung D
    et al.
    Kling-Bäckhed, Helene
    Giannakis, Marios
    Engstrand, Lars G
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Gordon, Jeffrey I
    Interactions between gastric epithelial stem cells and Helicobacter pylori in the setting of chronic atrophic gastritis.2006In: Curr Opin Microbiol, ISSN 1369-5274, Vol. 9, no 1, p. 21-7Article in journal (Other scientific)
  • 35. Olfat, Farzad O
    et al.
    Näslund, Erik
    Freedman, Jacob
    Borén, Thomas
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Cultured human gastric explants: a model for studies of bacteria-host interaction during conditions of experimental Helicobacter pylori infection.2002In: J Infect Dis, ISSN 0022-1899, Vol. 186, no 3, p. 423-7Article in journal (Other scientific)
  • 36. Olfat, Farzad O
    et al.
    Zheng, Quing
    Oleastro, Monica
    Voland, Petra
    Borén, Thomas
    Karttunen, Riita
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Rad, Roland
    Prinz, Christian
    Gerhard, Markus
    Correlation of the Helicobacter pylori adherence factor BabA with duodenal ulcer disease in four European countries.2005In: FEMS Immunol Med Microbiol, ISSN 0928-8244, Vol. 44, no 2, p. 151-6Article in journal (Refereed)
  • 37. Rahman, Motiur
    et al.
    Mukhopadhyay, Asish K
    Nahar, Shamsun
    Datta, Simanti
    Ahmad, Milan Mashhud
    Sarker, Safique
    Masud, Ibna M
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Albert, M John
    Nair, G Balakrish
    Berg, Douglas E
    DNA-level characterization of Helicobacter pylori strains from patients with overt disease and with benign infections in Bangladesh.2003In: J Clin Microbiol, ISSN 0095-1137, Vol. 41, no 5, p. 2008-14Article in journal (Other scientific)
  • 38. Ronkainen, Jukka
    et al.
    Aro, Pertti
    Storskrubb, Tom
    Johansson, Sven-Erik
    Lind, Tore
    Bolling-Sternevald, Elisabeth
    Graffner, Hans
    Vieth, Michael
    Stolte, Manfred
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Talley, Nicholas J
    Agréus, Lars
    High prevalence of gastroesophageal reflux symptoms and esophagitis with or without symptoms in the general adult Swedish population: a Kalixanda study report.2005In: Scand J Gastroenterol, ISSN 0036-5521, Vol. 40, no 3, p. 275-85Article in journal (Refereed)
  • 39. Roos, Stefan
    et al.
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Jonsson, Hans
    Lactobacillus gastricus sp. nov., Lactobacillus antri sp. nov., Lactobacillus kalixensis sp. nov. and Lactobacillus ultunensis sp. nov., isolated from human stomach mucosa.2005In: Int J Syst Evol Microbiol, ISSN 1466-5026, Vol. 55, no Pt 1, p. 77-82Article in journal (Other scientific)
  • 40. Schirwitz, Katja
    et al.
    Lundin, Annelie
    Skoglund, Anna
    Krabbe, Margareta
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Enroth, Cristofer
    Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of HP1352, a putative DNA methyltransferase in Helicobacter pylori.2003In: Acta Crystallogr D Biol Crystallogr, ISSN 0907-4449, Vol. 59, no Pt 4, p. 719-20Article in journal (Other scientific)
  • 41. Simá¡n, J Henrik
    et al.
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Berglund, Göran
    Florén, Claes-Henrik
    Forsgren, Arne
    Evaluation of western blot CagA seropositivity in Helicobacter pylori-seropositive and -seronegative subjects.2005In: Clin Diagn Lab Immunol, ISSN 1071-412X, Vol. 12, no 2, p. 304-9Article in journal (Refereed)
  • 42.
    Sjölund, Maria
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
    Wreiber, Karin
    Andersson, Dan I
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences.
    Blaser, Martin J
    Engstrand, Lars
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
    Long-term persistence of resistant Enterococcus species after antibiotics to eradicate Helicobacter pylori2003In: Annals of Internal Medicine, ISSN 0003-4819, E-ISSN 1539-3704, Vol. 139, no 6, p. 483-487Article in journal (Other academic)
    Abstract [en]

    BACKGROUND:

    Antibiotic treatment selects for resistance not only in the pathogen to which it is directed but also in the indigenous microflora.

    OBJECTIVE:

    To determine whether a widely used regimen (clarithromycin, metronidazole, and omeprazole) for Helicobacter pylori eradication affects resistance development in enterococci.

    DESIGN:

    Cohort study.

    SETTING:

    Endoscopy units at 3 community hospitals in Sweden.

    PATIENTS:

    5 consecutive dyspeptic patients who were colonized with H. pylori, had endoscopy-confirmed duodenal ulcer, and received antibiotic treatment, and 5 consecutive controls with dyspepsia but no ulcer who did not receive treatment.

    MEASUREMENTS:

    Fecal samples were obtained from patients and controls before, immediately after, 1 year after, and 3 years after treatment. From each patient and sample, enterococci were isolated and analyzed for DNA fingerprint, clarithromycin susceptibility, and presence of the erm(B) gene.

    RESULTS:

    In treated patients, all enterococci isolated immediately after treatment showed high-level clarithromycin resistance due to erm(B). In 3 patients, resistant enterococci persisted for 1 to 3 years after treatment. No resistance developed among controls.

    CONCLUSION:

    A common H. pylori treatment selects for highly resistant enterococci that can persist for at least 3 years without further selection.

  • 43.
    Storm, Martin
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Bacteriology.
    Gustafsson, Ingegerd
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Bacteriology.
    Herrmann, Björn
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Bacteriology.
    Engstrand, Lars
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Bacteriology.
    Real-time PCR for pharmacodynamic studies of Chlamydia trachomatis2005In: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 61, no 3, p. 361-367Article in journal (Refereed)
  • 44. Talley, N J
    et al.
    Vakil, N
    Delaney, B
    Marshall, B
    Bytzer, P
    Engstrand, L
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    de Boer, W
    Jones, R
    Malfertheiner, P
    Agréus, L
    Management issues in dyspepsia: current consensus and controversies.2004In: Scand J Gastroenterol, ISSN 0036-5521, Vol. 39, no 10, p. 913-8Article in journal (Other scientific)
  • 45. Tezcan-Merdol, Dilek
    et al.
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Rhen, Mikael
    Salmonella enterica SpvB-mediated ADP-ribosylation as an activator for host cell actin degradation.2005In: Int J Med Microbiol, ISSN 1438-4221, Vol. 295, no 4, p. 201-12Article in journal (Other scientific)
  • 46. Tezcan-Merdol, Dilek
    et al.
    Ljungström, Marianne
    Winiecka-Krusnell, Jadwiga
    Linder, Ewert
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Rhen, Mikael
    Uptake and replication of Salmonella enterica in Acanthamoeba rhysodes.2004In: Appl Environ Microbiol, ISSN 0099-2240, Vol. 70, no 6, p. 3706-14Article in journal (Other scientific)
  • 47. Wahab, Tara
    et al.
    Hjalmarsson, Sandra
    Wollin, Ralfh
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Pyrosequencing Bacillus anthracis.2005In: Emerg Infect Dis, ISSN 1080-6040, Vol. 11, no 10, p. 1527-31Article in journal (Other scientific)
  • 48. Westergren, Viveka
    et al.
    Bassiri, Mansour
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Bacteria detected by culture and 16S rRNA sequencing in maxillary sinus samples from intensive care unit patients.2003In: Laryngoscope, ISSN 0023-852X, Vol. 113, no 2, p. 270-5Article in journal (Other scientific)
  • 49.
    Willen, R
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
    Wanders, Alkwin
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
    Engstrand, L
    Intestinal spirochaetosis2006In: Histopathology, ISSN 0309-0167, E-ISSN 1365-2559, Vol. 49, no 6, p. 658-659Article in journal (Refereed)
  • 50. Winiecka-Krusnell, Jadwiga
    et al.
    Wreiber, Karin
    von Euler, Ann
    Engstrand, Lars
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
    Linder, Ewert
    Free-living amoebae promote growth and survival of Helicobacter pylori.2002In: Scand J Infect Dis, ISSN 0036-5548, Vol. 34, no 4, p. 253-6Article in journal (Other scientific)
12 1 - 50 of 53
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