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  • 1.
    Junaid, Sara
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics. Univ Punjab, Dept Microbiol & Mol Genet, Quaid e Azam Campus, Lahore 54590, Pakistan;Women Univ Multan, Dept Microbiol & Mol Genet, Multan, Pakistan.
    Khanna, Namita
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics. Birla Inst Technol & Sci Pilani, Dept Biotechnol, Dubai Campus, Dubai, U Arab Emirates.
    Lindblad, Peter
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Ahmed, Mehboob
    Univ Punjab, Dept Microbiol & Mol Genet, Quaid e Azam Campus, Lahore 54590, Pakistan.
    Multifaceted biofuel production by microalgal isolates from Pakistan2019In: Biofuels, Bioproducts and Biorefining, ISSN 1932-104X, E-ISSN 1932-1031, Vol. 13, no 5, p. 1187-1201Article in journal (Refereed)
    Abstract [en]

    Third-generation biofuels are currently considered to be the most resourceful medium for generating bioenergy. In the present study, microalgal strains were isolated from soil samples collected in Pakistan and characterized by 18S rRNA sequencing. The strains were identified as green algae Gloeocystis sp. MFUM-4, Sphaerocystis sp. MFUM-34, and Dictyochloropsis sp. MFUM-35. They were further studied for their potential to produce popular biofuels such as biodiesel, bioethanol, and biohydrogen. Under the test conditions, Gloeocystis sp. MFUM-4 emerged as the most suitable candidate, amongst the three new isolates, for biofuel production with a biodiesel production potential of 33.3% (w/v). Eight different environmental conditions were also tested to identify the most suitable condition for biohydrogen and bioethanol production using the newly isolated strains. Under light but in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), Gloeocystis recorded the highest capacity to produce both biohydrogen and bioethanol compared with the other strains that were examined.

  • 2.
    Khanna, Namita
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Perspectives on Algal Engineering for Enhanced Biofuel Production2016In: Algal Biorefinery: An Integrated Approach / [ed] Debabrata Das, Cham: Springer Publishing Company, 2016, p. 73-101Chapter in book (Refereed)
    Abstract [en]

    Algae as photoautotrophs can trap the solar energy and convert it into usable form. Solar energy is the most abundant and ultimate energy source. The total amount of solar energy absorbed by the Earth’s surface is 1.74 × 105 terawatts (TW) (Bhattacharya S et al., Biochem Biotechnol 120:159–167, 2005), which is a tremendous amount as compared to the world’s energy consumption (~13 TW) (Walter JM et al., Curr Opin Biotechnol 21:265–270, 2010). Therefore, conversion of solar energy to fuels may constitute the most sustainable way to solve the energy crisis.

  • 3.
    Khanna, Namita
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Esmieu, Charlène
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Meszaros, Livia S.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Lindblad, Peter
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Berggren, Gustav
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    In vivo activation of an [FeFe] hydrogenase using synthetic cofactors2017In: Energy & Environmental Science, ISSN 1754-5692, E-ISSN 1754-5706, Vol. 10, no 7, p. 1563-1567Article in journal (Refereed)
    Abstract [en]

    [FeFe] hydrogenases catalyze the reduction of protons, and oxidation of hydrogen gas, with remarkable efficiency. The reaction occurs at the H-cluster, which contains an organometallic [2Fe] subsite. The unique nature of the [2Fe] subsite makes it dependent on a specific set of maturation enzymes for its biosynthesis and incorporation into the apo-enzyme. Herein we report on how this can be circumvented, and the apo-enzyme activated in vivo by synthetic active site analogues taken up by the living cell.

  • 4.
    Khanna, Namita
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Lindblad, Peter
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Cyanobacterial Hydrogenases and Hydrogen Metabolism Revisited:: Recent Progress and Future Prospects2015In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 16, no 5, p. 10537-10561Article, review/survey (Refereed)
    Abstract [en]

    Cyanobacteria have garnered interest as potential cell factories for hydrogen production. In conjunction with photosynthesis, these organisms can utilize inexpensive inorganic substrates and solar energy for simultaneous biosynthesis and hydrogen evolution. However, the hydrogen yield associated with these organisms remains far too low to compete with the existing chemical processes. Our limited understanding of the cellular hydrogen production pathway is a primary setback in the potential scale-up of this process. In this regard, the present review discusses the recent insight around ferredoxin/flavodoxin as the likely electron donor to the bidirectional Hox hydrogenase instead of the generally accepted NAD(P)H. This may have far reaching implications in powering solar driven hydrogen production. However, it is evident that a successful hydrogen-producing candidate would likely integrate enzymatic traits from different species. Engineering the [NiFe] hydrogenases for optimal catalytic efficiency or expression of a high turnover [FeFe] hydrogenase in these photo-autotrophs may facilitate the development of strains to reach target levels of biohydrogen production in cyanobacteria. The fundamental advancements achieved in these fields are also summarized in this review.

  • 5.
    Khanna, Namita
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Raleiras, Patrícia
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Lindblad, Peter
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Fundamentals and Recent Advances in Hydrogen Production and Nitrogen Fixation in Cyanobacteria2016In: The Physiology of Microalgae / [ed] Michael A. Borowitzka, John Beardall, John A. Raven, Switzerland: Springer International Publishing , 2016, p. 101-127Chapter in book (Other academic)
  • 6.
    Khetorn, Wanthanee
    et al.
    Chulalongkorn University, Thailand.
    Khanna, Namita
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Incharoensakdi, Aran
    Chulalongkorn University, Thailand.
    Lindblad, Peter
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Metabolic and genetic engineering of cyanobacteria for enhanced hydrogen production2013In: Biofuels, ISSN 1759-7269, Vol. 4, no 5, p. 535-561Article in journal (Refereed)
    Abstract [en]

    There is an urgent need to develop sustainable solutions to convert solar energy into energy carriers used in the society. In addition to solar cells generating electricity, there are several options to generate solar fuels with molecular hydrogen (H2) being an interesting and promising option. Native and engineered cyanobacteria have been used as model systems to examine, develop and demonstrate photobiological hydrogen production. In the present review we present and discuss recent progress with respect to (i) native biological systems to generate hydrogen, (ii) metabolic modulations, and (iii) genetic engineering of metabolic pathways, as well as the (iv) introduction of custom-designed, non-native enzymes and complexes for enhanced hydrogen production in cyanobacteria. In conclusion, metabolic and genetic engineering of native cyanobacterial hydrogen metabolism can significantly increase the hydrogen production, and introduction of custom-designed non-native capacities open up new possibilities to further enhance cyanobacterial based hydrogen production.

  • 7.
    Lindblad, Peter
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Fuente, David
    Univ Politecn Valencia, Inst Aplicac Tecnol Informac & Comunicac Avanzada, Valencia, Spain.
    Borbe, Friederike
    KSD Innovat GmbH, Werksstr 15, D-45527 Hattingen, Germany.
    Cicchi, Bernardo
    CNR, Ist Valorizzaz Legno & Specie Arboree, Via Madonna Piano 10, I-50019 Florence, Italy.
    Conejero, J. Alberto
    Univ Politecn Valencia, Inst Univ Matemat Pura & Aplicada, Valencia, Spain.
    Couto, Narciso
    Univ Sheffield, Dept Chem & Biol Engn, ChELSI Inst, Sheffield, S Yorkshire, England.
    Celesnik, Helena
    Univ Ljubljana, Fac Chem & Chem Technol, Vecna Pot 113, SI-1000 Ljubljana, Slovenia.
    Diano, Marcello M.
    Business Innovat Ctr, Sci Ctr, M2M Engn Sas, Via Coroglio, Naples, Italy.
    Dolinar, Marko
    Univ Ljubljana, Fac Chem & Chem Technol, Vecna Pot 113, SI-1000 Ljubljana, Slovenia.
    Esposito, Serena
    Business Innovat Ctr, Sci Ctr, M2M Engn Sas, Via Coroglio, Naples, Italy.
    Evans, Caroline
    Univ Sheffield, Dept Chem & Biol Engn, ChELSI Inst, Sheffield, S Yorkshire, England.
    Ferreira, Eunice A.
    Univ Porto, i3S, Porto, Portugal;Univ Porto, IBMC, Porto, Portugal;Univ Porto, ICBAS, Porto, Portugal.
    Keller, Joseph
    Ruhr Univ Bochum, Fac Biol & Biotechnol, Plant Biochem, D-44780 Bochum, Germany.
    Khanna, Namita
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Kind, Gabriel
    Univ Appl Sci, Biotechnol & Chem, Technikumpl 17, DE-09648 Mittweida, Germany.
    Landels, Andrew
    Univ Sheffield, Dept Chem & Biol Engn, ChELSI Inst, Sheffield, S Yorkshire, England;Plymouth Marine Lab, Prospect Pl, Plymouth PL1 3DH, Devon, England.
    Lemus, Lenin
    Univ Politecn Valencia, Inst Aplicac Tecnol Informac & Comunicac Avanzada, Valencia, Spain.
    Noirel, Josselin
    Conservatoire Natl Arts & Metiers, LGBA, F-75003 Paris, France.
    Ocklenburg, Sarah
    KSD Innovat GmbH, Werksstr 15, D-45527 Hattingen, Germany.
    Oliveira, Paulo
    Univ Porto, i3S, Porto, Portugal;Univ Porto, IBMC, Porto, Portugal.
    Pacheco, Catarina C.
    Univ Porto, i3S, Porto, Portugal;Univ Porto, IBMC, Porto, Portugal.
    Parker, Jennifer L.
    Univ Sheffield, Dept Chem & Biol Engn, ChELSI Inst, Sheffield, S Yorkshire, England.
    Pereira, Jose
    Univ Porto, i3S, Porto, Portugal;Univ Porto, IBMC, Porto, Portugal.
    Khoa Pham, T.
    Univ Sheffield, Dept Chem & Biol Engn, ChELSI Inst, Sheffield, S Yorkshire, England.
    Pinto, Filipe
    Univ Porto, i3S, Porto, Portugal;Univ Porto, IBMC, Porto, Portugal.
    Rexroth, Sascha
    Ruhr Univ Bochum, Fac Biol & Biotechnol, Plant Biochem, D-44780 Bochum, Germany.
    Roegner, Matthias
    Ruhr Univ Bochum, Fac Biol & Biotechnol, Plant Biochem, D-44780 Bochum, Germany.
    Schmitz, Hans-Juergen
    KSD Innovat GmbH, Werksstr 15, D-45527 Hattingen, Germany.
    Silva Benavides, Ana Margarita
    Univ Costa Rica, Escuela Biol, San Jose 11501, Costa Rica;Univ Costa Rica, Ctr Invest Ciencias Mar & Limnol CIMAR, San Jose 11501, Costa Rica.
    Siurana, Maria
    Univ Politecn Valencia, Inst Univ Matemat Pura & Aplicada, Valencia, Spain.
    Tamagnini, Paula
    Univ Porto, i3S, Porto, Portugal;Univ Porto, IBMC, Porto, Portugal;Univ Porto, Dept Biol, Fac Ciencias, Porto, Portugal.
    Touloupakis, Eleftherios
    CNR, Ist Ric Ecosistemi Terr, Via Madonna Piano 10, I-50019 Sesto Fiorentino, Italy.
    Torzillo, Giuseppe
    CNR, Ist Valorizzaz Legno & Specie Arboree, Via Madonna Piano 10, I-50019 Florence, Italy.
    Urchueguia, Javier F.
    Univ Politecn Valencia, Inst Aplicac Tecnol Informac & Comunicac Avanzada, Valencia, Spain.
    Wegelius, Adam
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Wiegand, Katrin
    Ruhr Univ Bochum, Fac Biol & Biotechnol, Plant Biochem, D-44780 Bochum, Germany.
    Wright, Phillip C.
    Univ Sheffield, Dept Chem & Biol Engn, ChELSI Inst, Sheffield, S Yorkshire, England;Newcastle Univ, Fac Sci Agr & Engn, Fac Off, Sch Chem Engn & Adv Mat, Newcastle, NSW NE1 7RU, Australia.
    Wutschel, Mathias
    KSD Innovat GmbH, Werksstr 15, D-45527 Hattingen, Germany.
    Wuenschiers, Roebbe
    Univ Appl Sci, Biotechnol & Chem, Technikumpl 17, DE-09648 Mittweida, Germany.
    CyanoFactory, a European consortium to develop technologies needed to advance cyanobacteria as chassis for production of chemicals and fuels2019In: Algal Research, ISSN 2211-9264, Vol. 41, article id 101510Article, review/survey (Refereed)
    Abstract [en]

    CyanoFactory, Design, construction and demonstration of solar biofuel production using novel (photo) synthetic cell factories, was an R&D project developed in response to the European Commission FP7-ENERGY-2012-1 call "Future Emerging Technologies" and the need for significant advances in both new science and technologies to convert solar energy into a fuel. CyanoFactory was an example of "purpose driven" research and development with identified scientific goals and creation of new technologies. The present overview highlights significant outcomes of the project, three years after its successful completion. The scientific progress of CyanoFactory involved: (i) development of a ToolBox for cyanobacterial synthetic biology; (ii) construction of DataWarehouse/Bioinformatics web-based capacities and functions; (iii) improvement of chassis growth, functionality and robustness; (iv) introduction of custom designed genetic constructs into cyanobacteria, (v) improvement of photosynthetic efficiency towards hydrogen production; (vi) biosafety mechanisms; (vii) analyses of the designed cyanobacterial cells to identify bottlenecks with suggestions on further improvements; (viii) metabolic modelling of engineered cells; (ix) development of an efficient laboratory scale photobioreactor unit; and (x) the assembly and experimental performance assessment of a larger (1350 L) outdoor flat panel photobioreactor system during two seasons. CyanoFactory - Custom design and purpose construction of microbial cells for the production of desired products using synthetic biology - aimed to go beyond conventional paths to pursue innovative and high impact goals. CyanoFactory brought together ten leading European partners (universities, research organizations and enterprises) with a common goal - to develop the future technologies in Synthetic biology and Advanced photobioreactors.

  • 8.
    Magnuson, Ann
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Raleiras, Patricia
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Meszaros, Livia S.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Khanna, Namita
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Miranda, Helder
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Ho, Felix M.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Lindblad, Peter
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Styring, Stenbjörn
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Sustainable photobiological hydrogen production via protein engineering of cyanobacterial hydrogenases2018In: Abstract of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 255Article in journal (Other academic)
  • 9.
    Miao, Rui
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Wegelius, Adam
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Durall de la Fuente, Claudia
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Liang, Feiyan
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Khanna, Namita
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Lindblad, Peter
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Engineering Cyanobacteria for Biofuel Production2017In: Modern Topics in the Phototrophic Prokaryotes: Environmental and Applied Aspects / [ed] Hallenbeck, Patrick, USA: Springer, 2017, p. 351-393Chapter in book (Refereed)
  • 10.
    Raleiras, Patrícia
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Khanna, Namita
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Miranda, Helder
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Meszaros, Livia S.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Krassen, Henning
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Ho, Felix
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Battchikova, Natalia
    Turku University.
    Aro, Eva-Mari
    Turku University.
    Magnuson, Ann
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Lindblad, Peter
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Styring, Stenbjörn
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Turning around the electron flow in an uptake hydrogenase. EPR spectroscopy and in vivo activity of a designed mutant in HupSL from Nostoc punctiforme2016In: Energy & Environmental Science, ISSN 1754-5692, E-ISSN 1754-5706, Vol. 9, no 2, p. 581-594Article in journal (Refereed)
    Abstract [en]

    The filamentous cyanobacterium Nostoc punctiforme ATCC 29133 produces hydrogen via nitrogenase in heterocysts upon onset of nitrogen-fixing conditions. N. punctiforme expresses concomitantly the uptake hydrogenase HupSL, which oxidizes hydrogen in an effort to recover some of the reducing power used up by nitrogenase. Eliminating uptake activity has been employed as a strategy for net hydrogen production in N. punctiforme (Lindberg et al., Int. J. Hydrogen Energy, 2002, 27, 1291-1296). However, nitrogenase activity wanes within a few days. In the present work, we modify the proximal iron-sulfur cluster in the hydrogenase small subunit HupS by introducing the designed mutation C12P in the fusion protein f-HupS for expression in E. coli (Raleiras et al., J. Biol. Chem., 2013, 288, 18345-18352), and in the full HupSL enzyme for expression in N. punctiforme. C12P f-HupS was investigated by EPR spectroscopy and found to form a new paramagnetic species at the proximal cluster site consistent with a [4Fe-4S] to [3Fe-4S] cluster conversion. The new cluster has the features of an unprecedented mixed-coordination [3Fe-4S] metal center. The mutation was found to produce stable protein in vitro, in silico and in vivo. When C12P HupSL was expressed in N. punctiforme, the strain had a consistently higher hydrogen production than the background [capital Delta]hupSL mutant. We conclude that the increase in hydrogen production is due to the modification of the proximal iron-sulfur cluster in HupS, leading to a turn of the electron flow in the enzyme.

  • 11.
    Wegelius, Adam
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Khanna, Namita
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Esmieu, Charlene
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Barone, Giovanni Davide
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Pinto, Filipe
    Univ Porto, IBMC, I3S, P-4200135 Porto, Portugal;Univ Porto, Fac Ciencias, Dept Biol, P-4169007 Porto, Portugal;Univ Edinburgh, Sch Biol Sci, Edinburgh EH9 3FF, Midlothian, Scotland;Univ Edinburgh, Ctr Synthet & Syst Biol, Edinburgh EH9 3FF, Midlothian, Scotland.
    Tamagnini, Paula
    Univ Porto, IBMC, I3S, P-4200135 Porto, Portugal;Univ Porto, Fac Ciencias, Dept Biol, P-4169007 Porto, Portugal.
    Berggren, Gustav
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Lindblad, Peter
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
    Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism2018In: Energy & Environmental Science, ISSN 1754-5692, E-ISSN 1754-5706, Vol. 11, no 11, p. 3163-3167Article in journal (Refereed)
    Abstract [en]

    [FeFe]-Hydrogenases are hydrogen producing metalloenzymes with excellent catalytic capacities, highly relevant in the context of a future hydrogen economy. Here we demonstrate the synthetic activation of a heterologously expressed [FeFe]-hydrogenase in living cells of Synechocystis PCC 6803, a photoautotrophic microbial chassis with high potential for biotechnological energy applications. H-2-Evolution assays clearly show that the non-native, semi-synthetic enzyme links to the native metabolism in living cells.

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