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  • 1.
    Amato, Letizia
    et al.
    Technical University of Denmark.
    Heiskanen, Arto
    Technical University of Denmark.
    Hansen, Rasmus
    Technical University of Denmark.
    Gammelgaard, Lauge
    Capres A/S.
    Rindzevicius, Tomas
    Technical University of Denmark.
    Tenje, Maria
    Lunds universitet.
    Emnéus, Jenny
    Technical University of Denmark.
    Keller, Stephan
    Technical University of Denmark.
    Dense high-aspect ratio 3D carbon pillars on interdigitated microelectrode arrays2015In: Carbon, ISSN 0008-6223, E-ISSN 1873-3891, Vol. 94, p. 792-803Article in journal (Refereed)
  • 2.
    Andersson, Marlene
    et al.
    Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden..
    Jia, Qiupin
    Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, P.R. China..
    Abella, Ana
    ETSI de Caminos and Center for Biomedical Technology, Universidad Politécnica de Madrid, Madrid, Spain..
    Lee, Xiau-Yeen
    ETSI de Caminos and Center for Biomedical Technology, Universidad Politécnica de Madrid, Madrid, Spain..
    Landreh, Michael
    Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK..
    Purhonen, Pasi
    Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden.; School of Technology and Health, KTH Royal Institute of Technology, Stockholm, Sweden..
    Hebert, Hans
    Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden.; School of Technology and Health, KTH Royal Institute of Technology, Stockholm, Sweden..
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Department of Biomedical Engineering, Lund University, Lund, Sweden..
    Robinson, Carol V.
    Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK..
    Meng, Qing
    Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, P.R. China..
    Plaza, Gustavo R.
    ETSI de Caminos and Center for Biomedical Technology, Universidad Politécnica de Madrid, Madrid, Spain..
    Johansson, Jan
    Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden.; Department of Neurobiology, Care Sciences and Society (NVS), Karolinska Institutet, Stockholm, Sweden.; Karolinska Institutet.
    Rising, Anna
    Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden.;Department of Neurobiology, Care Sciences and Society (NVS), Karolinska Institutet, Stockholm, Sweden.; Karolinska Institutet.
    Biomimetic spinning of artificial spider silk from a chimeric minispidroin2017In: Nature Chemical Biology, ISSN 1552-4450, E-ISSN 1552-4469, Vol. 13, no 3, p. 262-264Article in journal (Refereed)
    Abstract [en]

    Herein we present a chimeric recombinant spider silk protein (spidroin) whose aqueous solubility equals that of native spider silk dope and a spinning device that is based solely on aqueous buffers, shear forces and lowered pH. The process recapitulates the complex molecular mechanisms that dictate native spider silk spinning and is highly efficient; spidroin from one liter of bacterial shake-flask culture is enough to spin a kilometer of the hitherto toughest as-spun artificial spider silk fiber.

  • 3.
    Evander, Mikael
    et al.
    Lund University.
    Tenje, Maria
    Lund University.
    Microfluidic PMMA interfaces for rectangular glass capillaries2014In: Journal of Micromechanics and Microengineering, ISSN 0960-1317, E-ISSN 1361-6439, Vol. 24, no 2, p. 027003-Article in journal (Refereed)
  • 4.
    Fornell, Anna
    et al.
    Lund Univ, Dept Biomed Engn, Lund, Sweden..
    Cushing, Kevin
    Lund Univ, Dept Biomed Engn, Lund, Sweden..
    Nilsson, Johan
    Lund Univ, Dept Biomed Engn, Lund, Sweden..
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund Univ, Dept Biomed Engn, Lund, Sweden.
    Binary particle separation in droplet microfluidics using acoustophoresis2018In: Applied Physics Letters, ISSN 0003-6951, E-ISSN 1077-3118, Vol. 112, no 6, article id 063701Article in journal (Refereed)
    Abstract [en]

    We show a method for separation of two particle species with different acoustic contrasts originally encapsulated in the same droplet in a continuous two-phase system. This was realized by using bulk acoustic standing waves in a 380 mu m wide silicon-glass microfluidic channel. Polystyrene particles (positive acoustic contrast particles) and in-house synthesized polydimethylsiloxane (PDMS) particles (negative acoustic contrast particles) were encapsulated inside water-in-oil droplets either individually or in a mixture. At acoustic actuation of the system at the fundamental resonance frequency, the polystyrene particles were moved to the center of the droplet (pressure node), while the PDMS particles were moved to the sides of the droplet (pressure anti-nodes). The acoustic particle manipulation step was combined in series with a trifurcation droplet splitter, and as the original droplet passed through the splitter and was divided into three daughter droplets, the polystyrene particles were directed into the center daughter droplet, while the PDMS particles were directed into the two side daughter droplets. The presented method expands the droplet microfluidics tool-box and offers new possibilities to perform binary particle separation in droplet microfluidic systems.

    The full text will be freely available from 2019-03-27 14:49
  • 5.
    Fornell, Anna
    et al.
    Department of Biomedical Engineering, Lund University, Sweden.
    Cushing, Kevin
    Department of Biomedical Engineering, Lund University, Lund, Sweden.
    Nilsson, Johan
    Department of Biomedical Engineering, Lund University, Lund, Sweden.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Intra-droplet acoustic separation of two particle species in a droplet microfluidic system2017Conference paper (Refereed)
  • 6.
    Fornell, Anna
    et al.
    Lund University.
    Garofalo, Fabio
    Lund University.
    Nilsson, Johan
    Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund University.
    Acoustophoretic particle manipulation in droplet microfluidics at higher resonance modes2016Conference paper (Refereed)
    Abstract [en]

    In this paper we investigate focusing of microparticles in the presence of multiple pressure nodes inside aqueous droplets by using bulk acoustic waves. The microfluidic chip s for droplet generation and particle encapsulation (within the droplets) were fabricated using anisotropic wet - etching of a silicon wafer. Subsequently, piezoelectric transducers featuring different thicknesses were glued on the chips to build the final devices. The transducer thicknesses were chosen as to match the acoustic resonances of the embedded micro channel at the fundamental frequency, the first and the second harmonics. The actuation of the devices at the first three resonance modes enabled the positioning of the microparticles in one, two or three bands, in accordance with the presence of pressure nodes within the droplet contained in the microchannel. This acoustic particle manipulation technique opens up for new possibilities to perform biological assays using droplet microfluidic platforms.

  • 7.
    Fornell, Anna
    et al.
    Lund University.
    Garofalo, Fabio
    Lund University.
    Nilsson, Johan
    Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund University.
    Experimental investigation of resonance conditions for particle focusing in droplet acoustofluidics2016Conference paper (Refereed)
    Abstract [en]

    We have experimentally studied the conditions for acoustic particle focusing inside aqueous droplets in two-phase-systems, and confirmed our findings by theoretical analysis. The results show that the acoustic properties of the two fluids have to be matched to achieve strong acoustic resonance and focusing in the system.

  • 8. Fornell, Anna
    et al.
    Joensson, Haakan
    Antfolk, Maria
    Nilsson, Johan
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Focusing microparticles inside droplets using acoustics2015Conference paper (Refereed)
  • 9.
    Fornell, Anna
    et al.
    Lund University.
    Joensson, Haakan
    Royal Institute of Technology (KTH).
    Nilsson, Johan
    Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Acoustic focusing of microparticles in two-phase systems: Towards cell enrichment or medium exchange in droplets2015In: 19th International Conference on Miniaturised Systems for Chemistry and Life Sciences (µTAS 2015), Gyeongju, Korea, October 25-29 2015, 2015Conference paper (Refereed)
  • 10. Fornell, Anna
    et al.
    Nilsson, Johan
    Jonsson, Linus
    Periyannan Rajeswari, Prem
    Joensson, Haakan
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Controlled lateral positioning of microparticles inside droplets using acoustophoresis2015In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 87, no 20, p. 10521-10526Article in journal (Refereed)
    Abstract [en]

    In this paper, we utilize bulk acoustic waves to control the position of microparticles inside droplets in two-phase microfluidic systems and demonstrate a method to enrich the microparticles. In droplet microfluidics, different unit operations are combined and integrated on-chip to miniaturize complex biochemical assays. We present a droplet unit operation capable of controlling the position of microparticles during a trident shaped droplet split. An acoustic standing wave field is generated in the microchannel, and the acoustic forces direct the encapsulated microparticles to the center of the droplets. The method is generic, requires no labeling of the microparticles, and is operated in a noncontact fashion. It was possible to achieve 2+-fold enrichment of polystyrene beads (5 μm in diameter) in the center daughter droplet with an average recovery of 89% of the beads. Red blood cells were also successfully manipulated inside droplets. These results show the possibility to use acoustophoresis in two-phase systems to enrich microparticles and open up the possibility for new droplet-based assays that are not performed today.

  • 11.
    Fornell, Anna
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Nilsson, Johan
    Jonsson, Linus
    Periyannan Rajeswari, Prem
    Joensson, Haakan
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Particle enrichment in droplet acoustofluidics2016Conference paper (Refereed)
  • 12.
    Fornell, Anna
    et al.
    Lund University.
    Nilsson, Johan
    Lund University.
    Jonsson, Linus
    Lund University.
    Periyannan Rajeswari, Prem
    KTH, Science for Life Laboratory.
    Joensson, Haakan
    KTH, Science for Life Laboratory.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund University.
    Particle enrichment in two-phase microfluidic systems using acoustophoresis2016Conference paper (Refereed)
  • 13.
    Fornell, Anna
    et al.
    Lund University.
    Ohlin, Mathias
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Garofalo, Fabio
    Department Biomedical Engineering, Lund University.
    Nilsson, Johan
    Department Biomedical Engineering, Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Department Biomedical Engineering, Lund University.
    An intra-droplet particle switch for droplet microfluidics using bulk acoustic waves2017In: Biomicrofluidics, ISSN 1932-1058, E-ISSN 1932-1058, Vol. 11, article id 031101Article in journal (Refereed)
    Abstract [en]

    To transfer cell- and bead-assays into droplet-based platforms typically requires the use of complex microfluidic circuits, which calls for methods to switch the direction of the encapsulated particles. We present a microfluidic chip where the combination of acoustic manipulation at two different harmonics and a trident-shaped droplet-splitter enables direction-switching of microbeads and yeast cells in droplet microfluidic circuits. At the first harmonic, the encapsulated particles exit the splitter in the center daughter droplets, while at the second harmonic, the particles exit in the side daughter droplets. This method holds promises for droplet-based assays where particle-positioning needs to be selectively controlled.

  • 14.
    Fornell, Anna
    et al.
    Department of Biomedical Engineering, Lund University, Sweden.
    Ohlin, Mathias
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Nilsson, Johan
    Department Biomedical Engineering, Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    A droplet unit operator for controlled particle switching and enrichment2017Conference paper (Refereed)
  • 15.
    Fornell, Anna
    et al.
    Department of Biomedical Engineering, Lund University, Sweden.
    Ohlin, Mathias
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Nilsson, Johan
    Department of Biomedical Engineering, Lund University, Sweden.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    An optimized droplet split designed for acoustic intra-droplet particle enrichment2017Conference paper (Refereed)
  • 16.
    Jocic, Simonne
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Mestres, Gemma
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Cross-linked gelatin/agarose conjugate as a thermostable and biocompatible microfluidic material2016Conference paper (Refereed)
  • 17.
    Jocic, Simonne
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Mestres, Gemma
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund University, Dept. Biomedical Engineering, Lund 221 00, Sweden.
    Fabrication of user-friendly and biomimetic 1,1′-carbonyldiimidazole cross-linked gelatin/agar microfluidic devices2017In: Materials science & engineering. C, biomimetic materials, sensors and systems, ISSN 0928-4931, E-ISSN 1873-0191, Vol. 76, p. 1175-1180Article in journal (Refereed)
    Abstract [en]

    We have developed a straightforward technique for fabricating user-friendly and biomimetic microfluidic devices out of a gelatin/agar gel cross-linked with 1,1′-carbonyldiimidazole. The fabrication procedure requires only inexpensive starting materials such as glass capillaries and wires to mold 3D cylindrical channels into the gel with the possibility of achieving channel diameters of 375 μm and 1000 μm. We demonstrate that the channel absent of gel injury can retain fluid within its dimensions for at least 7 h. We also show that the device material does not autofluoresce nor provide hindrances with fluorescent imaging. A discussion of the chemical linkage identities of cross-linked gelatin/agar is included via ATR-FTIR studies. Crosslinking of the gelatin/agar is further confirmed by the lack of a gel to sol transition at physiological temperature as assessed by DSC measurements. SEM micrographs that demonstrate the 100 nm mean pore width of the cross-linked gelatin/agar are provided. This device is considered biomimetic because it represents components present in the natural extracellular matrix such as collagen and proteoglycans in the form of cross-linked gelatin/agar.

  • 18.
    Jonsson, Linus
    et al.
    Lund University.
    Fornell, Anna
    Lund University.
    Joensson, Haakan
    Royal Institute of Technology.
    Nilsson, Johan
    Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Development of a droplet generator towards applications using acoustophoretic sorting2014Conference paper (Other academic)
  • 19.
    Lenshof, Andreas
    et al.
    Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Lundgren, Maria
    Skåne University Hospital.
    Svärd-Nilsson, Ann-Margret
    Skåne University Hospital.
    Kjeldsen-Kragh, Jens
    Skåne University Hospital.
    Åberg, Lena
    Skåne University Hospital.
    Laurell, Thomas
    Lund University.
    Removal of proteins from blood using acoustophoresis2014Conference paper (Refereed)
  • 20.
    Mestres, Gemma
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Espanol, Montserrat
    Department of Engineering Sciences and Metallurgy, Technical University of Catalonia, Diagonal 647, 08028 Barcelona, Spain.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Tenje, Maria
    Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Department of Biomedical Engineering, Lund University, Box 118, 221 00 Lund, Sweden.
    Ott, Marjam
    Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Evaluation of Biocompatibility and Release of Reactive Oxygen Species of Aluminum Oxide-Coated Materials2016In: ACS Omega, ISSN 2470-1343, Vol. 1, no 4, p. 706-713Article in journal (Refereed)
    Abstract [en]

    Surface properties of biomaterials can strongly influence biomaterial−host interactions. For this reason, coating processes open a wide range of possibilities to modulate the fate of a biomaterial in the body. This study evaluates the effect of a coating material intended for drug delivery capsules on biocompatibility and the release of reactive oxygen species (ROS), that is, respiratory burst in macrophages that indicates acute inflammation. In parallel with a new approach to develop drug-delivery capsules by directly coating solid-state drug particles, in this study, glass slides and silicon nanoparticles (NPs) were coated with aluminum oxide (Al2O3) using atomic layer deposition. Different sizes of NPs (20 and 310 nm) were suspended at different concentrations (10, 100, and 1000 μg/mL) and were evaluated. The homogeneous coating of slides was proved using X-ray photoelectron spectroscopy, and the coating on NP was observed using transmission electron microscopy. Human dermal fibroblasts and human osteoblasts were able to proliferate on the coated slides and in the presence of a suspension of coated NPs (20 and 310 nm) at a low concentration (10 μg/mL). The macrophages released ROS only when in contact with NPs at a concentration of 1000 μg/mL, where the 20 nm NPs caused a higher release of ROS than the 310 nm NPs. This study shows that Al2O3 coatings do not affect the cells negatively and that the cell viability was compromised only when in contact with a high concentration (1000 μg/mL) of smaller (20 nm) NPs. 

  • 21.
    Mestres, Gemma
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Sjögren, Frida
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Thumula, Venkata
    Wolff, Anette
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Microstructured 3D cell culture scaffolds2016Conference paper (Refereed)
  • 22.
    Mestres, Gemma
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Wolff, Anette
    Hilborn, Jöns
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Microfluidic system with integrated 3D cell culture matrix: towards more in vivo-like organ-on-chip models2016Conference paper (Refereed)
  • 23.
    Montazerolghaem, Maryam
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Ning, Yi
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Karlsson Ott, Marjam
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Mestres, Gemma
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Simvastatin and zinc synergistically enhance osteoblasts activityand decrease the acute response of inflammatory cells2016In: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 27, no 2, article id 23Article in journal (Refereed)
    Abstract [en]

    Several ceramic biomaterials have been suggested as promising alternatives to autologous bone to replace or restore bone after trauma or disease. The osteoinductive potential of most scaffolds is often rather low by themselves and for this reason growth factors or drugs have been supplemented to these synthetic materials. Although some growth factors show good osteoinductive potential their drawback is their high cost and potential severe side effects. In this work the combination of the well-known drug simvastatin (SVA) and the inorganic element Zinc (Zn) is suggested as a potential additive to bone grafts in order to increase their bone regeneration/ formation. MC3T3-E1 cells were cultured with Zn (10 and 25 mu M) and SVA (0.25 and 0.4 mu M) for 10 days to evaluate proliferation and differentiation, and for 22 days to evaluate secretion of calcium deposits. The combination of Zn (10 mu M) and SVA (0.25 mu M) significantly enhanced cell differentiation and mineralization in a synergetic manner. In addition, the release of reactive oxygen species (ROS) from primary human monocytes in contact with the same concentrations of Zn and SVA was evaluated by chemiluminescence. The combination of the additives decreased the release of ROS, although Zn and SVA separately caused opposite effects. This work shows that a new combination of additives can be used to increase the osteoinductive capacity of porous bioceramics.

  • 24.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Andersson, Martin
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Klintberg, Lena
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Hjort, Klas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    In situ temperature monitoring during acoustophoresis using integrated thin film Pt temperature sensors2017Conference paper (Refereed)
  • 25.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Andersson, Martin
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Klintberg, Lena
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Hjort, Klas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Internal temperature sensing in an acoustophoretic glass chip2017Conference paper (Refereed)
  • 26.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Lund University, Lund, Sweden.
    Bruus, Henrik
    Tech Univ Denmark, Lyngby, Denmark.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund University, Lund, Sweden.
    Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis2017In: Journal of Micromechanics and Microengineering, ISSN 0960-1317, E-ISSN 1361-6439, Vol. 27, no 8, article id 084002Article in journal (Refereed)
    Abstract [en]

    We have fabricated a silicon-glass two-phase droplet microfluidic system capable of generating sub 100 µm-sized,   =  (74  ±  2) µm, spherical droplets at rates of up to hundreds of hertz. By implementing a two-dimensional (2D) acoustophoresis particle-positioning method, we show a fourfold improvement in both vertical and lateral particle positioning inside the droplets compared to unactuated operation. The efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37 °C). Furthermore, by using acoustic actuation, (99.8  ±  0.4)% of all encapsulated microparticles can be detected compared to only (79.0  ±  5.1)% for unactuated operation. In our experiments we observed a strong ordering of the microparticles in distinct patterns within the droplet when using 2D acoustophoresis; to explain the origin of these patterns we simulated numerically the fluid flow inside the droplets and compared with the experimental findings.

  • 27.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Department of Biomedical Engineering, Lund University, Sweden.
    Bruus, Henrik
    Department of Physics, Technical University of Denmark, Kgs. Lyngby, Denmark.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Using two-dimensional acoustophoresis for improved particle positioning in droplet microfluidics2017Conference paper (Refereed)
  • 28.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Department of Biomedical Engineering, Lund University, Sweden .
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Department of Biomedical Engineering, Lund University, Sweden .
    2D acoustophoretic positioning of microparticles inside droplets2016Conference paper (Refereed)
  • 29.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Fabrication of a silicon-glass microfluidic device for generation of sub 100-micrometer-sized droplets2016Conference paper (Refereed)
  • 30.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Department of Biomedical Engineering, Lund University, Sweden .
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Department of Biomedical Engineering, Lund University, Sweden .
    Two-dimensional acoustic focusing of microparticles in two-phase droplet-based microfluidic systems for improved particle positioning within spherical droplets2016Conference paper (Refereed)
    Abstract [en]

    We have fabricated a silicon-glass microfluidic two-phase droplet generator capable of generating sub 100-micrometer-sized (⌀ ൌ74 μm ± 2 μm) spherical droplets at rates up to hundreds of hertz (298 Hz ± 85 Hz). Furthermore, we have implemented a two-dimensional acoustic focusing technique into the device. Here, we show that applying the focusing to 10 μm sized polystyrene particles during the droplet generation step, results in a fourfold improvement of the particle positioning (centricity) within the generated droplets compared to the unactuated control. Finally, the efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37°C).

  • 31.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund University.
    Two-dimensional acoustic focusing of microparticles in two-phase droplet-based microfluidic systems increases particle detectability2016Conference paper (Refereed)
    Abstract [en]

    We have fabricated a silicon-glass two-phase droplet-based microfluidic system and implemented two-dimensional acoustic focusing prior to droplet generation as well as continuously throughout the whole system to increase particle detectability. Using acoustic focusing we have effectivelyminimized sedimentation of the encapsulated particles and thereby increased particle detectability by as much as 44% compared to unactuated operation of the system.

  • 32. Paloschi, Valentina
    et al.
    Mestres, Gemma
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Björk, Hanna
    Wolff, Anette
    Bhattachariya, Anirban
    Eriksson, Per
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Aorta-on-a-chip as a tool to study the interface intima-media in the aortic wall2016Conference paper (Refereed)
  • 33.
    Porras, Ana Maria
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Sjögren, Frida
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Shi, Liyang
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
    Ossipov, Dmitri A.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Dept. of Biomedical Engineering, Lund University, Lund, Sweden..
    Addressing the biocompatibility of photo-crosslinkable hyaluronic acid hydrogels2017In: Abstract book at EMBEC 2017 & NBC 2017, 2017, p. 117-117Conference paper (Refereed)
  • 34.
    Porras, Ana Maria
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Sjögren, Frida
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Shi, Liyang
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
    Ossipov, Dmitri A.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. SciLifeLab. Lund University.
    Photopatterning of hyaluronic acid hydrogels for cell culture scaffolds2016Conference paper (Refereed)
    Abstract [en]

    Organs-on-chips technologies require the development of micro engineered devices to represent functional units of human organs. These devices use cell culture scaffolds to give support and structure for the cultured cells. Hydrogels are attractive scaffold materials, due to their high water content and because they are derived from natural polymers found in the extracellular matrix of different tissues in the human body. Hyaluronic acid can form hydrogels when functionalized with chemo-selective groups. These chemically cross-linked hydrogels can be modified with adhesion motifs, such as RGD peptides, to increase their biocompatibility and promote cell adhesion. In this work we use a photolithographic method to pattern the RGD peptide into distinct areas of the hyaluronic acid hydrogel with the aim to spatially control the cell attachment on the HA hydrogel scaffolds

  • 35.
    Quan, Xueling
    et al.
    Technical University of Denmark.
    Heiskanen, Arto
    Technical University of Denmark.
    Grutter, Peter
    McGill University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Boisen, Anja
    Technical University of Denmark.
    Development of an electrochemical cantilever platform2014Conference paper (Other academic)
  • 36.
    Quan, Xueling
    et al.
    Technical University of Denmark.
    Heiskanen, Arto
    Technical University of Denmark.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Boisen, Anja
    Technical University of Denmark.
    Characterization of Electromechanical Behavior of an Electrochemical Cantilever System2014Conference paper (Other academic)
  • 37.
    Quan, Xueling
    et al.
    Technical University of Denmark.
    Heiskanen, Arto
    Technical University of Denmark.
    Tenje, Maria
    Lund University.
    Boisen, Anja
    Technical University of Denmark.
    In-situ monitoring of potential enhanced DNA related processes using electrochemical quartz crystal microbalance with dissipation (EQCM-D)2014In: Electrochemistry communications, ISSN 1388-2481, E-ISSN 1873-1902, Vol. 48, p. 111-114Article in journal (Refereed)
  • 38.
    Sjögren, Frida
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Bermejo, Daniel
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
    Sehlstedt, Viktor
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Hilborn, Jöns
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
    Ossipov, Dmitri
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Micropatterning of photocrosslinkable hyaluronic acid2015In: 26th Micromechanics and Microsystems Europe workshop (MME 2015), Toledo, Spain, September 20-23 2015, 2015Conference paper (Refereed)
  • 39.
    Sjögren, Frida
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Ossipov, Dmitri A.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Lund University.
    Swelling properties of photocrosslinkable hyaluronic acid2016Conference paper (Refereed)
  • 40.
    Sjögren, Frida
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Porras, Ana Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Shi, Liyang
    Ossipov, Dmitri
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Spatial and chemical patterning of hyaluronic acid using UV lithography2016Conference paper (Refereed)
  • 41.
    Tenje, Maria
    et al.
    Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala Univ, Dept Engn Sci, Sci Life Lab, S-75121 Uppsala, Sweden.;Lund Univ, Dept Biomed Engn, S-22363 Lund, Sweden..
    Fornell, Anna
    Lund Univ, Dept Biomed Engn, S-22363 Lund, Sweden..
    Ohlin, Mathias
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala Univ, Dept Engn Sci, S-75121 Uppsala, Sweden..
    Nilsson, Johan
    Lund Univ, Dept Biomed Engn, Lund, Sweden..
    Particle Manipulation Methods in Droplet Microfluidics2018In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 90, no 3, p. 1434-1443Article in journal (Refereed)
  • 42.
    Tenje, Maria
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    N. Lundgren, Maria
    Skane Univ Hosp, Dept Clin Immunol & Transfus Med, Lund, Sweden.
    Swärd-Nilsson, Ann-Margret
    Skane Univ Hosp, Dept Clin Immunol & Transfus Med, Lund, Sweden.
    Kjelsen-Kragh, Jens
    Skane Univ Hosp, Dept Clin Immunol & Transfus Med, Lund, Sweden.
    Lyxe, Lena
    Sahlgrens Univ Hosp, Gothenburg, Sweden.
    Lenshof, Andreas
    Lund Univ, Dept Biomed Engn, Lund, Sweden.
    Acoustophoretic removal of proteins from blood components2015In: Biomedical microdevices (Print), ISSN 1387-2176, E-ISSN 1572-8781, Vol. 17, no 5, article id UNSP 95Article in journal (Refereed)
    Abstract [en]

    This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97 %, compared to just above 90 % of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications.

  • 43.
    Tenje, Maria
    et al.
    Lund University.
    Xia, Hongyan
    Swedish University of Agricultural Sciences.
    Evander, Mikael
    Lund University.
    Hammarström, Björn
    Lund University.
    Tojo, Axel
    Lund University.
    Belák, Sándor
    National Veterinary Institute.
    Laurell, Thomas
    Lund University.
    LeBlanc, Neil
    National Veterinary Institute.
    Acoustic trapping as a generic non-contact incubation site for multiplex bead-based assays2015In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 853, p. 682-688Article in journal (Refereed)
  • 44.
    Tenje, Maria
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Xia, Hongyan
    Swedish University of Agricultural Sciences.
    Evander, Mikael
    Lund University.
    Hammarström, Björn
    Lund University.
    Tojo, Axel
    Lund University.
    Belák, Sándor
    National Veterinary Institute.
    Laurell, Thomas
    Lund University.
    LeBlanc, Neil
    National Veterinary Institute.
    Increased bead recovery using an acoustic trap as incubation site in multiplex assays2014Conference paper (Other academic)
  • 45.
    Tenje, Maria
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Xia, Hongyan
    Swedish University of Agricultural Sciences.
    Evander, Mikael
    Lund University.
    Hammarström, Björn
    Lund University.
    Tojo, Axel
    Lund University.
    Belák, Sándor
    National Veterinary Institute.
    Laurell, Thomas
    Lund University.
    LeBlanc, Neil
    National Veterinary Institute.
    Non-contact acoustic trapping platform for bead incubation for multiplex assays2014Conference paper (Refereed)
  • 46.
    Wolff, Anette
    et al.
    Lund University.
    Antfolk, Maria
    Lund University.
    Brodin, Birger
    Copenhagen University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    In vitro blood-brain barrier models: An overview of established models and new microfluidic approaches2015In: Journal of Pharmaceutical Sciences, ISSN 0022-3549, E-ISSN 1520-6017, Vol. 104, no 9, p. 2727-2746Article in journal (Refereed)
    Abstract [en]

    The societal need for new central nervous system (CNS) medicines is substantial, because of the global increase in life expectancy and the accompanying increase in age-related CNS diseases. Low blood-brain barrier (BBB) permeability has been one of the major causes of failure for new CNS drug candidates. There has therefore been a great interest in cell models, which mimic BBB permeation properties. In this review, we present an overview of the performance of monocultured, cocultured, and triple-cultured primary cells and immortalized cell lines, including key parameters such as transendothelial electrical resistance values, permeabilities of paracellular flux markers, and expression of BBB-specific marker proteins. Microfluidic systems are gaining ground as a new automated technical platform for cell culture and systematic analysis. The performance of these systems was compared with current state-of-the-art models and it was noted that, although they show great promise, these systems have not yet reached beyond the proof-of-concept stage. In general, it was found that there were large variations in experimental protocols, BBB phenotype markers, and paracellular flux markers used. It is the author's opinion that the field may benefit greatly from developing standardized methodologies and initiating collaborative efforts on optimizing culture protocols.

  • 47. Wolff, Anette
    et al.
    Brodin, Birger
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Evaluation of a novel seeding technique in microfluidic chips2015In: 26th Micromechanics and Microsystems Europe workshop (MME 2015), Toledo, Spain, September 20-23 2015, 2015Conference paper (Refereed)
  • 48.
    Wolff, Anette
    et al.
    Dept. Biomedical Engineering Lund University, Lund.
    Thomée, Emma
    Dept. Biomedical Engineering Lund University, Lund..
    Wallman, Lars
    Dept. Biomedical Engineering Lund University, Lund..
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Assembly of a microfluidic device using only plasma activation2017Conference paper (Refereed)
    Abstract [en]

    The blood-brain barrier (BBB) is a complex system, and essential for normal brainfunction as it protects the brain from harmful substances and changes in the blood composition.To analyse the BBB, the simplest way is by using in vitro models, and by utilising microfluidicdevices it is possible to integrate several parameters and analysis steps into one device. We havedeveloped a stable and reproducible bonding protocol for assembling a PDMS-based microfluidicdevice using only plasma activation, to remove the dimensional uncertainty of using intermediatelayer adhesives.

  • 49.
    Wolff, Anette
    et al.
    Dept. Biomedical Engineering Lund University, Lund.
    Thomée, Emma
    Dept. Biomedical Engineering Lund University, Lund..
    Wallman, Lars
    Dept. Biomedical Engineering Lund University, Lund..
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Optimisation of a glass/PDMS/PDMS/glass microfluidic chip assembly using air plasma2017Conference paper (Refereed)
1 - 49 of 49
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