uu.seUppsala University Publications
Change search
Refine search result
1 - 14 of 14
CiteExportLink to result list
Permanent link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Rows per page
  • 5
  • 10
  • 20
  • 50
  • 100
  • 250
Sort
  • Standard (Relevance)
  • Author A-Ö
  • Author Ö-A
  • Title A-Ö
  • Title Ö-A
  • Publication type A-Ö
  • Publication type Ö-A
  • Issued (Oldest first)
  • Issued (Newest first)
  • Created (Oldest first)
  • Created (Newest first)
  • Last updated (Oldest first)
  • Last updated (Newest first)
  • Disputation date (earliest first)
  • Disputation date (latest first)
  • Standard (Relevance)
  • Author A-Ö
  • Author Ö-A
  • Title A-Ö
  • Title Ö-A
  • Publication type A-Ö
  • Publication type Ö-A
  • Issued (Oldest first)
  • Issued (Newest first)
  • Created (Oldest first)
  • Created (Newest first)
  • Last updated (Oldest first)
  • Last updated (Newest first)
  • Disputation date (earliest first)
  • Disputation date (latest first)
Select
The maximal number of hits you can export is 250. When you want to export more records please use the Create feeds function.
  • 1.
    Fornell, Anna
    et al.
    Lund University.
    Ohlin, Mathias
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Garofalo, Fabio
    Department Biomedical Engineering, Lund University.
    Nilsson, Johan
    Department Biomedical Engineering, Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Department Biomedical Engineering, Lund University.
    An intra-droplet particle switch for droplet microfluidics using bulk acoustic waves2017In: Biomicrofluidics, ISSN 1932-1058, E-ISSN 1932-1058, Vol. 11, article id 031101Article in journal (Refereed)
    Abstract [en]

    To transfer cell- and bead-assays into droplet-based platforms typically requires the use of complex microfluidic circuits, which calls for methods to switch the direction of the encapsulated particles. We present a microfluidic chip where the combination of acoustic manipulation at two different harmonics and a trident-shaped droplet-splitter enables direction-switching of microbeads and yeast cells in droplet microfluidic circuits. At the first harmonic, the encapsulated particles exit the splitter in the center daughter droplets, while at the second harmonic, the particles exit in the side daughter droplets. This method holds promises for droplet-based assays where particle-positioning needs to be selectively controlled.

  • 2.
    Fornell, Anna
    et al.
    Department of Biomedical Engineering, Lund University, Sweden.
    Ohlin, Mathias
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Nilsson, Johan
    Department Biomedical Engineering, Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    A droplet unit operator for controlled particle switching and enrichment2017Conference paper (Refereed)
  • 3.
    Fornell, Anna
    et al.
    Department of Biomedical Engineering, Lund University, Sweden.
    Ohlin, Mathias
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Nilsson, Johan
    Department of Biomedical Engineering, Lund University, Sweden.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    An optimized droplet split designed for acoustic intra-droplet particle enrichment2017Conference paper (Refereed)
  • 4.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Andersson, Martin
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Klintberg, Lena
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Hjort, Klas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    In situ temperature monitoring during acoustophoresis using integrated thin film Pt temperature sensors2017Conference paper (Refereed)
  • 5.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Andersson, Martin
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Klintberg, Lena
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Hjort, Klas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Internal temperature sensing in an acoustophoretic glass chip2017Conference paper (Refereed)
  • 6.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Lund University, Lund, Sweden.
    Bruus, Henrik
    Tech Univ Denmark, Lyngby, Denmark.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund University, Lund, Sweden.
    Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis2017In: Journal of Micromechanics and Microengineering, ISSN 0960-1317, E-ISSN 1361-6439, Vol. 27, no 8, article id 084002Article in journal (Refereed)
    Abstract [en]

    We have fabricated a silicon-glass two-phase droplet microfluidic system capable of generating sub 100 µm-sized,   =  (74  ±  2) µm, spherical droplets at rates of up to hundreds of hertz. By implementing a two-dimensional (2D) acoustophoresis particle-positioning method, we show a fourfold improvement in both vertical and lateral particle positioning inside the droplets compared to unactuated operation. The efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37 °C). Furthermore, by using acoustic actuation, (99.8  ±  0.4)% of all encapsulated microparticles can be detected compared to only (79.0  ±  5.1)% for unactuated operation. In our experiments we observed a strong ordering of the microparticles in distinct patterns within the droplet when using 2D acoustophoresis; to explain the origin of these patterns we simulated numerically the fluid flow inside the droplets and compared with the experimental findings.

  • 7.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Department of Biomedical Engineering, Lund University, Sweden.
    Bruus, Henrik
    Department of Physics, Technical University of Denmark, Kgs. Lyngby, Denmark.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Using two-dimensional acoustophoresis for improved particle positioning in droplet microfluidics2017Conference paper (Refereed)
  • 8.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Department of Biomedical Engineering, Lund University, Sweden .
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Department of Biomedical Engineering, Lund University, Sweden .
    2D acoustophoretic positioning of microparticles inside droplets2016Conference paper (Refereed)
  • 9.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Fabrication of a silicon-glass microfluidic device for generation of sub 100-micrometer-sized droplets2016Conference paper (Refereed)
  • 10.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Department of Biomedical Engineering, Lund University, Sweden .
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Department of Biomedical Engineering, Lund University, Sweden .
    Two-dimensional acoustic focusing of microparticles in two-phase droplet-based microfluidic systems for improved particle positioning within spherical droplets2016Conference paper (Refereed)
    Abstract [en]

    We have fabricated a silicon-glass microfluidic two-phase droplet generator capable of generating sub 100-micrometer-sized (⌀ ൌ74 μm ± 2 μm) spherical droplets at rates up to hundreds of hertz (298 Hz ± 85 Hz). Furthermore, we have implemented a two-dimensional acoustic focusing technique into the device. Here, we show that applying the focusing to 10 μm sized polystyrene particles during the droplet generation step, results in a fourfold improvement of the particle positioning (centricity) within the generated droplets compared to the unactuated control. Finally, the efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37°C).

  • 11.
    Ohlin, Mathias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fornell, Anna
    Lund University.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Lund University.
    Two-dimensional acoustic focusing of microparticles in two-phase droplet-based microfluidic systems increases particle detectability2016Conference paper (Refereed)
    Abstract [en]

    We have fabricated a silicon-glass two-phase droplet-based microfluidic system and implemented two-dimensional acoustic focusing prior to droplet generation as well as continuously throughout the whole system to increase particle detectability. Using acoustic focusing we have effectivelyminimized sedimentation of the encapsulated particles and thereby increased particle detectability by as much as 44% compared to unactuated operation of the system.

  • 12.
    Tenje, Maria
    et al.
    Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Lund University, Department of Biomedical Engineering.
    Fornell, Anna
    Lund University, Department of Biomedical Engineering.
    Ohlin, Mathias
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Nilsson, Johan
    Lund University, Department of Biomedical Engineering.
    Particle Manipulation Methods in Droplet Microfluidics2018In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 90, no 3, p. 1434-1443Article in journal (Refereed)
    Abstract [en]

    This Feature article describes the different particle manipulation techniques available in the droplet microfluidics tool-box to handle particles encapsulated inside droplets and to manipulate whole droplets. We address the advantages and disadvantages of the different techniques to guide new users.  

  • 13.
    Werr, Gabriel
    et al.
    Tech Univ Munich, Inst Med & Polymer Engn, D-80333 Munich, Germany.
    Khaji, Zahra
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Ohlin, Mathias
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Aimpoint AB, S-21375 Malmo, Sweden.
    Andersson, Martin
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Klintberg, Lena
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Searle, Sean
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Natl Univ Singapore, Dept Biomed Engn, Singapore 117583, Singapore.
    Hjort, Klas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Integrated thin film resistive sensors for in situ temperature measurements in an acoustic trap2019In: Journal of Micromechanics and Microengineering, ISSN 0960-1317, E-ISSN 1361-6439, Vol. 29, no 9, article id 095003Article in journal (Refereed)
    Abstract [en]

    This work presents an acoustic trap with integrated thin film sensors to monitor temperature variations during operation. The acoustic trap is wet-etched in glass with a thermally bonded glass lid and the thin-film sensors are integrated during fabrication. We evaluated the performance of the integrated temperature sensors and measured a temperature sensitivity of +/- 0.01 degrees C and confirmed that the read-out of the thin film sensors was not affected neither by the ionic conductivity of the solution nor the addition of microparticles into the acoustic trap. From the experiments we observed a temperature increase of the acoustic trap during operation as a result of the dissipative heating of the the piezoelectric element used to actuate the trap. We also showed that when external convective cooling was applied to the system, the temperature increase of the acoustic trap was higher than the temperature increase of the piezoelectric element itself. This shows the importance of using integrated temperature sensors in acoustic trapping to monitor the local environmental conditions.

  • 14.
    Werr, Gabriel
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Khaji, Zahra
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Ohlin, Mathias
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Andersson, Martin
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Klintberg, Lena
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Searle, Sean
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Department of Biomedical Engineering, National University of Singapore, Singapore 117583, Singapore.
    Hjort, Klas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology, Ångström Space Technology Centre (ÅSTC). Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
    Tenje, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
    Integrated thin film resistive sensors for in situ temperature measurements in an acoustic trap2019In: Acoustofluidics 2019: This annual meeting will be held in Twente, The Netherlands in 2019. This focused meeting is dedicated to exploring the science, engineering, and use of micro- to nanoscale acoustofluidics., 2019, p. 140-141Conference paper (Other academic)
    Abstract [en]

    This work presents an acoustic trap with integrated thin film sensors to monitor temperature variations during operation. The acoustic trap is wet-etched in glass with a thermally bonded glass lid and the thin-film sensors are integrated during fabrication. We evaluated the performance of the integrated temperature sensors and measured a temperature sensitivity of ±0.01 °C and confirmed that the read-out of the thin film sensors was not affected neither by the ionic conducitiviy of the solution nor the addition of microparticles into the acoustic trap. From the experiments we observed a temperature increase of the acoustic trap during operation as a result of the dissipative heating of the the piezoelectric element used to actuate the trap. We also showed that when external convective cooling was applied to the system, the temperature increase of the acoustic trap was higher than the temperature incresase of the piezoelectric element itself. This shows the importance of using integrated temperature sensors in acoustic trapping to monitor the environmental conditions.

1 - 14 of 14
CiteExportLink to result list
Permanent link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf