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  • 1.
    Abrahamsson, Maria
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, För teknisk-naturvetenskapliga fakulteten gemensamma enheter, Accelerator mass spectrometry group. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical Chemistry.
    Wolpher, Henriette
    Johansson, Olof
    Larsson, Jan
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical Chemistry.
    Kritikos, Mikael
    Eriksson, Lars
    Norrby, Per-Ola
    Bergquist, Jonas
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Sun, Licheng
    Åkermark, Björn
    Hammarström, Leif
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical Chemistry.
    A New Strategy for the Improvement of Photophysical Properties in Ruthenium(II) Polypyridyl Complexes: Synthesis and Photophysical and Electrochemical Characterization of Six Mononuclear Ruthenium(II) Bisterpyridine-Type Complexes2005In: Inorganic Chemistry, ISSN 0020-1669, E-ISSN 1520-510X, Vol. 44, no 9, p. 3215-3225Article in journal (Refereed)
  • 2.
    Berger, Rolf
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry. Oorganisk kemi.
    Bucur, Romulus V.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry. Oorganisk kemi.
    Diffusion in Copper Sulphides: An experimental study of chalcocite, chalcopyrite and bornite1996Report (Other scientific)
    Abstract [en]

    Diffusion measurements on three copper-containing sulphides have been performed by an electrochemical potentiometric method. -- The diffusion coefficient for chalcocite is in good agreement with values found previously, and a reasonable agreement is also found for chalcopyrite and bornite when our data are compared with values acquired at much higher temperatures with a different technique. The activation energies are remarkably similar for the three sulphides, considering that their relative errors are of a 10% magnitude, which indicates that the bonding strengths and the diffusion mechanisms are similar.

  • 3.
    Högberg, Hans
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry.
    Malm, JO
    Talyzin, A
    Norin, L
    Lu, J
    Jansson, Ulf
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Deposition of transition metal carbides and superlattices using C-60 as carbon source2000In: JOURNAL OF THE ELECTROCHEMICAL SOCIETY, ISSN 0013-4651, Vol. 147, no 9, p. 3361-3369Article in journal (Refereed)
    Abstract [en]

    Thin films of TiC, VC, and NbC have been deposited on MgO(001) by coevaporation of the metals and C-60 It was found that these metals induced a decomposition of the C-60 molecule and that carbide films can be formed at a temperature as low as 100 degrees

  • 4.
    Kukli, K
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry.
    Aarik, J
    Aidla, A
    Forsgren, K
    Sundqvist, J
    Hårsta, Anders
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Uustare, T
    Mändar, H
    Kiisler, A. -A
    Atomic layer deposition of tantalum oxide thin films from iodide precursor2001In: Chem. Mater., Vol. 13, p. 122-Article in journal (Refereed)
  • 5.
    Kukli, K
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry.
    Aidla, A
    Department of Materials Chemistry, Inorganic Chemistry.
    Aarik, J
    Department of Materials Chemistry, Inorganic Chemistry.
    Schuisky, M
    Department of Materials Chemistry, Inorganic Chemistry.
    Hårsta, Anders
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Ritala, M
    Department of Materials Chemistry, Inorganic Chemistry.
    Leskelä, M
    Department of Materials Chemistry, Inorganic Chemistry.
    Real time monitoring in atomic layer deposition of TiO2 from TiI4 and H2O-H2O22000In: Langmuir, Vol. 16, p. 8122-Article in journal (Refereed)
  • 6.
    Kukli, K.
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry.
    Ritala, M
    Department of Materials Chemistry, Inorganic Chemistry.
    Schuisky, M
    Department of Materials Chemistry, Inorganic Chemistry.
    Leskelä, M
    Department of Materials Chemistry, Inorganic Chemistry.
    Sajavaara, T
    Department of Materials Chemistry, Inorganic Chemistry.
    Keinonen, J
    Department of Materials Chemistry, Inorganic Chemistry.
    Uustarem, T
    Department of Materials Chemistry, Inorganic Chemistry.
    Hårsta, Anders
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Atomic layer deposition of titanium oxide from TiI4 and H2O22000In: Chem. Vap. Deposition, Vol. 6, p. 303-Article in journal (Refereed)
  • 7.
    Lee, SK
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry.
    Zetterling, CM
    Danielsson, E
    Ostling, M
    Palmquist, Jens-Petter
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Högberg, Hans
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Jansson, Ulf
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Electrical characterization of TiC ohmic contacts to aluminum ion implanted 4H-silicon carbide2000In: APPLIED PHYSICS LETTERS, ISSN 0003-6951, Vol. 77, no 10, p. 1478-1480Article in journal (Refereed)
    Abstract [en]

    We report on the investigation of epitaxial TiC ohmic contacts to Al ion implanted 4H-SiC. TiC ohmic contacts were formed by coevaporation of Ti and C-60 at low temperature (< 500 degrees C). A sacrificial silicon nitride (Si3N4) layer was deposited on th

  • 8.
    Lee, SK
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry.
    Zetterling, CM
    Ostling, M
    Palmquist, Jens-Petter
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Högberg, H
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Jansson, Ulf
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Low resistivity ohmic titanium carbide contacts to n- and p-type 4H-silicon carbide2000In: SOLID-STATE ELECTRONICS, ISSN 0038-1101, Vol. 44, no 7, p. 1179-1186Article in journal (Refereed)
    Abstract [en]

    Low resistivity Ohmic contacts of epitaxial titanium carbide to highly doped n- (1.3 x 10(19) cm(-3)) and p- (>10(20) cm(-3)) type epilayer on 4H-SiC were investigated. The titanium carbide contacts were epitaxially grown using coevaporation with an e-bea

  • 9.
    Liljegren, G
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry.
    Nyholm, Leif
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Electrochemically Controlled Solid Phase Microextraction and Preconcentration using Polypyrrole Coated Microarray Electrodes in a Flow System2003In: presented at the 26th International Symposium on Capillary Chromatography & Electrophoresis, Las Vegas, USA, May 18-22, 2003, 2003Conference paper (Refereed)
  • 10.
    Liljegren, Gustav
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry. Analytisk kemi.
    Dahlin, Andreas
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry. Analytisk kemi.
    Zettersten, Camilla
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry. Analytisk kemi.
    Bergquist, Jonas
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry. Analytisk kemi.
    Nyholm, Leif
    Department of Materials Chemistry, Inorganic Chemistry. Oorganisk kemi.
    On-line coupling of a microelectrode array equipped poly(dimethylsiloxane) microchip with an integrated graphite electrospray emitter for electrospray ionisation mass spectrometry2005In: Lab on a Chip, no 5, p. 1008-1016Article in journal (Refereed)
    Abstract [en]

    A novel method for the manufacturing of microchips for on-chip combinations of electrochemistry (EC) and sheathless electrospray ionisation mass spectrometry (ESI-MS) is described. The technique, which does not require access to clean-room facilities, is based on the incorporation of an array of gold microcoil electrodes into a poly(dimethylsiloxane) (PDMS) microflow channel equipped with an integrated graphite based sheathless ESI emitter. Electrochemical measurements, which were employed to determine the electroactive area of the electrodes and to test the microchips, show that the manufacturing process was reproducible and that the important interelectrode distance in the electrochemical cell could to be adequately controlled. The EC-ESI-MS device was evaluated based on the ESI-MS detection of the oxidation products of dopamine. The results demonstrate that the present on-chip approach enables full potentiostatic control of the electrochemical cell and the attainment of very short transfer times between the electrochemical cell and the electrospray emitter. The transfer times were 0.6 and 1.2 s for flow rates of 1.0 and 0.5 uL min-1, respectively, while the electrochemical conversion efficiency of the electrochemical cell was found to be 30% at a flow rate of 0.5 uL min-1. To decouple the electrochemical cell from the ESI-MS high voltage and to increase the user-friendliness, the on-line electrochemistry-ESI-MS experiments were performed using a wireless Bluetooth battery-powered instrument with the chip floating at the potential induced by the ESI high voltage. The described on-chip EC-ESI-MS device can be used for fundamental electrochemical investigations as well as for applications based on the use of electrochemically controlled sample pretreatment, preconcentration and ionisation steps prior to ESI-MS.

  • 11.
    Liljegren, Gustav
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry. Analytisk kemi.
    Forsgard, Niklas
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry. Analytisk kemi.
    Zettersten, Camilla
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry. Analytisk kemi.
    Pettersson, Jean
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry. Analytisk kemi.
    Svedberg, Malin
    Herranen, Merja
    Department of Materials Chemistry, Inorganic Chemistry. Oorganisk kemi.
    Nyholm, Leif
    Department of Materials Chemistry, Inorganic Chemistry. Oorganisk kemi.
    On-line electrochemically controlled solid-phase extraction interfaced to electrospray and inductively coupled plasma mass spectrometry2005In: The Analyst, no 130, p. 1358-1368Article in journal (Refereed)
    Abstract [en]

    Electrochemically controlled solid-phase extractions of anions were interfaced on-line to electrospray mass spectrometry (ESI-MS) and inductively coupled plasma mass spectrometry (ICP-MS), using polypyrrole coated electrodes and a thin-layer electrochemical (EC) flow cell. The results indicate that electrochemically controlled solid-phase extraction (EC-SPE) can be used as a versatile potential controlled sample preparation technique for a range of anions and that the properties of the polypyrrole coatings can be modified by altering the electrodeposition conditions. In the present study, the influence of interfering anions (i.e., fluoride and sulfate), and the anion used during the electropolymerisation, on the bromide extraction recovery was investigated for EC-SPE interfaced to ICP-MS. The results of these experiments show that the interference due to the presence of similar concentrations of sulfate can be reduced when using a polypyrrole coating electropolymerised in the presence of bromide ions. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) measurements were also used to study the morphology of the coatings, as well as the variations in the film thickness within the coatings. The effect of different desorption techniques on the bromide preconcentration factor in the ICP-MS on-line flow system was also examined. Stopped-flow desorption was found to give rise to significantly increased preconcentration factors in comparison with desorptions in flowing solutions. While the desorption efficiency depends on the type of desorption electrolyte (the electrolyte in which the desorption takes place), due to the competing influx of cations, the influence of the pH on the switching charge of the polypyrrole coating was found to be small, at constant ionic strength. To study the applicability of the EC-SPE technique with respect to real samples, investigations were also made with tap water samples spiked with different bromide concentrations. The results of these experiments, which were carried out using a modified thin-layer EC flow cell allowing in situ polymerisation of polypyrrole yielding a polymer plug covering the cross section of the channel, demonstrate that 3 uM concentrations of bromide could be detected in the tap water sample. This demonstrates that the extraction technique allows extractions of low concentrations of ions in the presence of significantly higher concentrations of other similar ions. The fact that the extraction and desorption steps are electrochemically controlled makes EC-SPE particularly well suited for inclusion in miniaturised lab-on-a-chip systems.

  • 12.
    Lu, J
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry, Inorganic Chemistry.
    Hugosson, H
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry, Inorganic Chemistry.
    Eriksson, Olle
    Nordström, Lars
    Jansson, Ulf
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry, Inorganic Chemistry.
    Chemical vapour deposition of molybdenum carbides: aspects of phase stability2000In: THIN SOLID FILMS, ISSN 0040-6090, Vol. 370, no 1-2, p. 203-212Article in journal (Refereed)
    Abstract [en]

    Thin films of different molybdenum carbides (delta-MoC1-x, gamma'-MoC1-x and Mo2C) have been deposited from a gas mixture of MoCl5/H-2/C2H4 at 800 degrees C by CVD. The H-2 content in the vapour has a strong influence on the phase composition and microstr

  • 13.
    Lundberg, N
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry.
    Ostling, M
    Zetterling, CM
    Tagtstrom, P
    Jansson, Ulf
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    CVD-based tungsten carbide Schottky contacts to 6H-SiC for very high-temperature operation2000In: JOURNAL OF ELECTRONIC MATERIALS, ISSN 0361-5235, Vol. 29, no 3, p. 372-375Article in journal (Refereed)
    Abstract [en]

    In this study, tungsten carbide, with its hardness, chemical inertness, thermal stability and low resistivity (25 mu Omega cm)(1) is shown as a reliable contact material to n- and p-type 6H-SiC for very high temperature applications. WC films with thickne

  • 14. Långström, B
    et al.
    Antoni, Gunnar
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry.
    Halldin, Christer
    Svärd, Hans
    Bergson, Göran
    Synthesis of some 11C-labelled alkaloids1982In: Chemica Scripta, Vol. 20, p. 46-49Article in journal (Refereed)
  • 15.
    Mume, Eskender
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry.
    Radiohalogenated Compounds for Tumor Targeting: Synthesis and Radiolabeling2005Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    This thesis describes the synthesis and radiohalogenation of compounds of potential use for tumor targeting.

    The first section describes the synthesis and radioiodination of DNA intercalating compounds. The compounds are derivatives of 9-aminoacridine, and the anthracyclins daunorubicin and doxorubicin. The precursor compounds were labeled with 125I (T1/2 = 60 days), which is an Auger emitting nuclide. 125I decaying in the close vicinity of DNA is known to have a much higher cell killing effect than 125I decaying in the cytoplasm and some of the labeled compounds prepared in this thesis are currently being tested for use in targeted radionuclide therapy for cancer.

    The second section describes the radiobromination of closo-carboranes by subjecting the corresponding iodinated compounds to palladium-catalyzed halogen exchange using [76Br]bromide. The 76Br isotope (T1/2 = 16.2 h) is a positron emitting nuclide that is suitable for PET studies. Via the halogen exchange reaction good to excellent radiochemical yields of radiobrominated closo-carboranes were obtained. The results of the present study may prove to be applicable to pharmacokinetic studies of carboranes and their derivatives.

    The third and final section describes the indirect radiobromination of the trastuzumab anti-HER2 monoclonal antibody and of the anti-HER2 Affibody by means of an “one-pot” procedure using N-succinimidyl-5-(tributylstannyl)-3-pyridinecarboxylate (SPC) and ((4-hydroxyphenyl)ethyl))maleimide (HPEM), respectively. It was found that SPC and HPEM can be efficiently radiobrominated and thereafter coupled to the antibody and Affibody, respectively. The labeled proteins retained their capacity to bind specifically to HER2 expressing SKOV-3 cells in vitro. Application of this method to 76Br might enable the use of PET in the detection of HER2 expression in breast, ovarian, and urinary bladder carcinomas.

    List of papers
    1. Synthesis and Radioiodination of Some 9-Aminoacridine Derivatives
    Open this publication in new window or tab >>Synthesis and Radioiodination of Some 9-Aminoacridine Derivatives
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    2004 (English)In: European Journal of Organic Chemistry, ISSN 1434-193X, E-ISSN 1099-0690, Vol. 17, p. 3719-3725Article in journal (Refereed) Published
    National Category
    Organic Chemistry
    Identifiers
    urn:nbn:se:uu:diva-69939 (URN)10.1002/ejoc.200400296 (DOI)
    Available from: 2006-05-17 Created: 2006-05-17 Last updated: 2017-11-21Bibliographically approved
    2. Synthesis and radioiodination of some 9-aminoacridine derivatives for potential use in radionuclide therapy
    Open this publication in new window or tab >>Synthesis and radioiodination of some 9-aminoacridine derivatives for potential use in radionuclide therapy
    Show others...
    2005 (English)In: Journal of labelled compounds & radiopharmaceuticals, ISSN 0362-4803, E-ISSN 1099-1344, Vol. 48, no 12, p. 855-871Article in journal (Refereed) Published
    National Category
    Organic Chemistry
    Identifiers
    urn:nbn:se:uu:diva-83610 (URN)10.1002/jlcr.960 (DOI)
    Available from: 2006-11-07 Created: 2006-11-07 Last updated: 2017-12-14Bibliographically approved
    3. Synthesis and radioiodination of some daunorubicin and doxorubicin derivatives
    Open this publication in new window or tab >>Synthesis and radioiodination of some daunorubicin and doxorubicin derivatives
    2005 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 340, no 1, p. 15-24Article in journal (Refereed) Published
    Abstract [en]

    Daunorubicin and doxorubicin are efficient agents for cancer treatment. Their clinical efficacy is, however, hampered by their indiscriminant toxicity. This problem may be circumvented by encapsulating the drugs in liposomes and selectively targeting the tumor cells using tumor targeting agents. Furthermore, the antitumor effect could be enhanced by attaching the Auger electron emitter, 125I, to daunorubicin an ddoxorubicin derivatives. In this context a number of ester, amide, and amine derivatives of daunorubicin an ddoxorubicin were synthesized. Benzoic acid ester derivatives of daunorubicin were synthesized by nucleophilic esterification of the 14-bromodaunorubicin with the potassium salt of the corresponding benzoic acid, resulting in good yields. Nicotinic acids and benzoic acids, activated with a succinimidyl group, were coupled to the amino group of daunorubicien to give the corresponding amide derivatives. Amine derivatives were obtained by the reductive amination of aromatic aldehydes with daunorubicin hydrochloride. The stannylated ester and amide derivatives were uses as precursors for radioiodination. Radiolabeling with 125I was performed using chloroamine-T as an oxidant. The optimized labeling resulted in high radiolabeling yields (85-95%) of the radioiodinated daunorubicin and doxorubicin derivatives. Radioiodination of the amines was conducted at the ortho position of the activated phenyl rings providing moderate radiochemical yields (55-75%).

    Keyword
    Daunorubicin, Doxorubicin, Radioiodination, 125I, Chloramine-T
    National Category
    Organic Chemistry
    Identifiers
    urn:nbn:se:uu:diva-70001 (URN)doi:10.1016/j.carres.2004.10.014 (DOI)15620662 (PubMedID)
    Available from: 2006-06-29 Created: 2006-06-29 Last updated: 2017-11-21Bibliographically approved
    4. Radiobromination of closo-carboranes using palladium-catalyzed halogen exchange
    Open this publication in new window or tab >>Radiobromination of closo-carboranes using palladium-catalyzed halogen exchange
    2005 (English)In: Journal of labelled compounds & radiopharmaceuticals, ISSN 0362-4803, E-ISSN 1099-1344, Vol. 48, no 3, p. 195-202Article in journal (Refereed) Published
    Keyword
    halogen exchange, carboranes, [76Br]bromide, labelling, palladium, catalyst
    National Category
    Organic Chemistry
    Identifiers
    urn:nbn:se:uu:diva-69999 (URN)10.1002/jlcr.914 (DOI)
    Available from: 2005-04-13 Created: 2005-04-13 Last updated: 2017-11-21Bibliographically approved
    5. Radiobromination of humanized anti-HER2 monoclonal antibody trastuzumab using N-succinimidyl 5-bromo-3-pyridinecarboxylate, a potential label for immunoPET
    Open this publication in new window or tab >>Radiobromination of humanized anti-HER2 monoclonal antibody trastuzumab using N-succinimidyl 5-bromo-3-pyridinecarboxylate, a potential label for immunoPET
    Show others...
    2005 (English)In: Nuclear Medicine and Biology, ISSN 0969-8051, E-ISSN 1872-9614, Vol. 32, no 6, p. 613-22Article in journal (Refereed) Published
    Abstract [en]

    Combining the specificity of radioimmunoscintigraphy and the high sensitivity of PET in an in vivo detection technique could improve the quality of nuclear diagnostics. Positron-emitting nuclide (76)Br (T(1/2)=16.2 h) might be a possible candidate for labeling monoclonal antibodies (mAbs) and their fragments, provided that the appropriate labeling chemistry has been established. For internalizing antibodies, such as the humanized anti-HER2 monoclonal antibody, trastuzumab, radiobromine label should be residualizing, i.e., ensuring that radiocatabolites are trapped intracellularly after the proteolytic degradation of antibody. This study evaluated the chemistry of indirect radiobromination of trastuzumab using N-succinimidyl 5-(tributylstannyl)-3-pyridinecarboxylate. Literature data indicated that the use of this method provided residualizing properties for iodine and astatine labels on some antibodies. An optimized "one-pot" procedure produced an overall labeling efficiency of 45.5+/-1.2% over 15 min. The bromine label was stable under physiological and denaturing conditions. The labeled trastuzumab retained its capacity to bind specifically to HER2-expressing SKOV-3 ovarian carcinoma cells in vitro (immunoreactivity more than 75%). However, in vitro cell test did not demonstrate that the radiobromination of trastuzumab using N-succinimidyl 5-bromo-3-pyridinecarboxylate improves cellular retention of radioactivity in comparison with the use of N-succinimidyl 4-bromobenzoate.

    National Category
    Medical and Health Sciences
    Identifiers
    urn:nbn:se:uu:diva-104548 (URN)10.1016/j.nucmedbio.2005.04.010 (DOI)16026708 (PubMedID)
    Available from: 2009-05-28 Created: 2009-05-28 Last updated: 2017-12-13Bibliographically approved
    6. Evaluation of ((4-Hydroxyphenyl)ethyl)maleimide for Site-Specific Radiobromination of Anti-HER2 Affibody
    Open this publication in new window or tab >>Evaluation of ((4-Hydroxyphenyl)ethyl)maleimide for Site-Specific Radiobromination of Anti-HER2 Affibody
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    2005 (English)In: Bioconjugate chemistry, ISSN 1043-1802, E-ISSN 1520-4812, Vol. 16, no 6, p. 1547-1555Article in journal (Refereed) Published
    Abstract [en]

    Affibody molecules are a new class of small phage-display selected proteins using a scaffold domain of the bacterial receptor protein A. They can be selected for specific binding to a large variety of protein targets. An affibody molecule binidng with high affinity to a tumor antigen HER2 was recently developed for radionuclide diagnostics and therapy in vivo. The use of hte positron-emitting nuclide 76Br(T½ = 16.2 h) could imporve the sensitivity of detection of HER2-expressing tumors. A site-specific radiobromination o fa cysteine-containing variant of the anti-HER2 affibody, (ZHER2:4)2-Cys, using ((4-hydroxpyphenyl)ethyl)maleimide (HPEM), was evaluated in this study. It was found that HPEM can be radiobrominated with an efficiency of 83+0.4% and thereafter coupled to freshly reduced conjugate to exceed 97%. The label was stable against challenge with large excess of nonlabeled bromide and in a high molar strengt solution. In vitro cell tests demonstraded that radiobrominated affibody binds specifically to the HER2-expressing cel-line, SK-OV-3. Biodistribution studies in nude mice bearing SK-OV-3 xenografts have shown tumor accumulation of 4.8 ? 2.2% IA/g and good tumor-to-normal tissue ratios.

    Keyword
    Animals, Antigens; Neoplasm/analysis/metabolism, Binding Sites, Bromine Radioisotopes/*chemistry, Cell Line; Tumor, Humans, Maleimides/chemistry, Mice, Mice; Nude, Neoplasms; Experimental/pathology, Peptide Library, Peptides/chemistry/*pharmacokinetics, Protein Interaction Mapping, Radiopharmaceuticals/*chemical synthesis/*pharmacokinetics, Receptor; erbB-2/analysis/*metabolism, Research Support; Non-U.S. Gov't, Staphylococcal Protein A/chemistry, Tissue Distribution, Transplantation; Heterologous
    National Category
    Organic Chemistry
    Identifiers
    urn:nbn:se:uu:diva-74804 (URN)10.1021/bc050056o (DOI)16287254 (PubMedID)
    Available from: 2006-06-29 Created: 2006-06-29 Last updated: 2017-12-14Bibliographically approved
  • 16.
    Pettersson Dahlin, Andreas
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Wetterhall, Magnus
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Liljegren, Gustav
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Bergström, Sara K.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Andrén, Per
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
    Nyholm, Leif
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry, Materials Chemistry.
    Markides, Karin E
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Bergquist, Jonas
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Capillary electrophoresis coupled to mass spectrometry from a polymer modified poly(dimethylsiloxane) microchip with an integrated graphite electrospray tip2005In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 130, no 2, p. 193-199Article in journal (Refereed)
    Abstract [en]

    Hybrid capillary-poly(dimethysiloxane) (PDMS) microchips with integrated electrospray ionization (ESI) tips were directly fabricated by casting PDMS in a mould. The shapes of the emitter tips were drilled into the mould, which produced highly reproducible three-dimensional tips. Due to the fabrication method of the microfluidic devices, no sealing was necessary and it was possible to produce a perfect channel modified by PolyE-323, an aliphatic polyamine coating agent. A variety of different coating procedures were also evaluated for the outside of the emitter tip. Dusting graphite on a thin unpolymerised PDMS layer followed by polymerisation was proven to be the most suitable procedure. The emitter tips showed excellent electrochemical properties and durabilities. The coating of the emitter was eventually passivated, but not lost, and could be regenerated by electrochemical means. The excellent electrochemical stability was further confirmed in long term electrospray experiments, in which the emitter sprayed continuously for more than 180 h. The PolyE-323 was found suitable for systems that integrate rigid fused silica and soft PDMS technology, since it simply could be applied successfully to both materials. The spray stability was confirmed from the recording of a total ion chromatogram in which the electrospray current exhibited a relative standard deviation of 3.9% for a 30 min run. CE-ESI-MS separations of peptides were carried out within 2 min using the hybrid PDMS chip resulting in similar efficiencies as for fused silica capillaries of the same length and thus with no measurable band broadening effects, originating from the PDMS emitter.

  • 17.
    Schuisky, M
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry.
    Hårsta, Anders
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Aidla, A
    Department of Materials Chemistry, Inorganic Chemistry.
    Kukli, K
    Department of Materials Chemistry, Inorganic Chemistry.
    Kiisler, A.A
    Department of Materials Chemistry, Inorganic Chemistry.
    Aarik, J
    Department of Materials Chemistry, Inorganic Chemistry.
    Atomic layer chemical vapor deposition of TiO2: Low-temperature epitaxy of rutile and anatase2000In: J. Electrochem. Soc., Vol. 147, p. 3319-Article in journal (Refereed)
  • 18.
    Schuisky, M
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry.
    Hårsta, Anders
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Khartsev, S
    Department of Materials Chemistry, Inorganic Chemistry.
    Grishin, A
    Department of Materials Chemistry, Inorganic Chemistry.
    Ferroelectric Bi4Ti3O12 thin films on Pt-coated silicon by halide chemical vapor deposition2000In: J. Appl. Phys., Vol. 88, p. 2819-Article in journal (Refereed)
  • 19.
    Schuisky, M
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry.
    Kukli, K
    Department of Materials Chemistry, Inorganic Chemistry.
    Ritala, M
    Department of Materials Chemistry, Inorganic Chemistry.
    Hårsta, Anders
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Leskelä, M
    Department of Materials Chemistry, Inorganic Chemistry.
    Atomic layer chemical vapour deposition in the Bi-Ti-O system2000In: Chem. Vap. Deposition, Vol. 6, p. 139-Article in journal (Refereed)
  • 20.
    Talyzin, A
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry.
    Jansson, Ulf
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    C-60 and C-70 solvates studied by Raman spectroscopy2000In: JOURNAL OF PHYSICAL CHEMISTRY B, ISSN 1089-5647, Vol. 104, no 21, p. 5064-5071Article in journal (Refereed)
    Abstract [en]

    The formation and phase transformations of C-60 and C-70 solvates with benzene, toluene, and n-hexane have been studied in-solution by Raman spectroscopy for the first time. It was found that C-60 and C-70 solvates exhibit Raman spectra which are quite di

  • 21.
    Talyzin, AV
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Materials Chemistry, Inorganic Chemistry.
    Tergenius, LE
    Jansson, Ulf
    Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Single-crystal growth of C70S8 - a new phase in the C-70-sulphur system2000In: JOURNAL OF CRYSTAL GROWTH, ISSN 0022-0248, Vol. 213, no 1-2, p. 63-69Article in journal (Refereed)
    Abstract [en]

    Large crystals (up to 2 x 1 x 0.5 mm) of a new C-70-sulphur compound were grown by evaporation of a benzene solution. The composition of this compound was determined by chemical analysis to C70S8. Single-crystal and X-ray powder diffraction experiments sh

  • 22.
    Thorslund, Sara
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
    Bergström, S
    Johanesson, N
    Pettersson, A
    Liljegren, G
    Magnusson, K
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
    Hallberg, M
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
    Klett, Oliver
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
    Bergquist,, Jonas
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Nyholm, Leif
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry.
    Markides, Karin
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry, Inorganic Chemistry. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Materials Science.
    Nyberg, F
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry, Inorganic Chemistry. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Materials Science.
    Nikolajeff, Fredrik
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
    Essential components in an integrated platform for on-site screening of anabolic androgenic steroids in biological samples2004In: Micro Total Analysis Systems 2004: Vol 1 / [ed] Laurell T; Nilsson J; Jensen K; Harrison DJ; Kutter JP, 2004Conference paper (Refereed)
    Abstract [en]

    In this abstract new results concerning the introduction of a biological sample into a mu TAS, the avoidance of unspecific absorbance in the mu TAS and the interfacing to off-chip detection (e.g. Mass spectrometry, MS) for verification are presented.

  • 23.
    Törnkvist, Anna
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Nilsson, Stefan
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Amirkhani, Ardeshir
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Nyholm, Lena
    Nyholm, Leif
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Interference of the electrospray voltage on chromatographic separations using porous graphitic carbon columns2004In: Journal of Mass Spectrometry, ISSN 1076-5174, E-ISSN 1096-9888, Vol. 39, no 2, p. 216-222Article in journal (Refereed)
    Abstract [en]

    The electrospray ionization (ESI) voltage is shown to interfere with liquid chromatographic separations performed with packed porous graphitic carbon (PGC) capillary columns. This interference is ascribed to the presence of an electric field over the conductive column in the absence of an earth point between the column and the ESI emitter. The current evolved alters the chromatographic behavior of the catecholamine metabolite 3-O-methyl-DOPA significantly, as both peak splitting and a dramatic decrease in the retention time were observed. Furthermore, the response from the mass spectrometer was decreased by 33% at the same time. A related compound, tyrosine, exhibited decreased retention times but no peak splitting, whereas no shifts in the retention times (or peak splitting) were seen for the less retained dopamine and noradrenaline. When the current through the PGC column was eliminated by the use of an earth point between the column and the ESI emitter, the chromatographic behavior of the column was found to return slowly to normal after hours of equilibration with 60 : 40 (v/v) methanol-ammonium formate buffer of pH 2.9. The behavior of the PGC column with and without the earth point was found to be highly reproducible during a period of 1 month. We propose that the effect of the ESI voltage on the chromatographic behavior of the PGC column is due to associated redox reactions affecting both the PGC particles and the analytes. It is concluded that (for analytical reasons), care should be taken to ensure that no current is flowing through the chromatographic system when interfacing PGC columns, and conducting parts in general, to ESI mass spectrometry.

  • 24.
    Zettersten, Camilla
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Lomoth, Reiner
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical Chemistry.
    Hammarström, Leif
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical Chemistry.
    Sjöberg, Per
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Nyholm, Leif
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry.
    The influence of the thin-layer flow cell design on the mass spectra when coupling electrochemistry to electrospray ionisation mass spectrometry2006In: Journal of Electroanalytical Chemistry, ISSN 0022-0728, E-ISSN 1873-2569, Vol. 590, no 1, p. 90-99Article in journal (Refereed)
    Abstract [en]

    The influence of the flow cell configuration on the mass spectra obtained when coupling an electrochemical thin-layer flow cell to electrospray mass spectrometry (ESI-MS) has been investigated. It is shown that interferences due to the electrochemical reaction on the counter electrode and/or the absence of 100% conversion efficiency can alter the mass spectra when conventional thin-layer flow cells are used in conjunction with ESI-MS. The effects, which affect the intensities and distribution of the peaks in the mass spectra, can result in the inability to detect products formed at the working electrode. Comparisons of mass spectra, generated after the electrochemical oxidation of a dinuclear Mn complex (where bpmp = 2,6-bis[bis(2-pyridylmethyl) amino]methyl-4-methylphenol) using two different thin-layer flow cells clearly show that the potential dependence and appearance of the mass spectra depend on the flow cell configuration used. The use of a modified thin-layer flow cell, in which the counter electrode had been separated from the working electrode, gave rise to significantly increased intensities for the oxidised MnIII,IV state of the complex. With the conventional unmodified cell, the corresponding complex was only seen for considerably higher oxidation potentials. The different results can be explained by the reduced risk of redox cycling and interferences due to species generated at the counter electrode with the modified cell. As interferences due to the counter electrode reactions likewise may be expected with many coulometric flow cells, the electrochemical cell design clearly needs to be considered when using electrochemistry coupled to ESI-MS to study electrochemical reactions.

1 - 24 of 24
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