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  • 1.
    Ahlgren, Joakim
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Tranvik, Lars
    Gogoll, Adolf
    Waldebäck, Monica
    Markides, Karin
    Rydin, Emil
    Depth attenuation of biogenic phosphorus compounds in lake sediment measured by 31P NMR2005In: Environmental Science and Technology, ISSN 0013-936, Vol. 39, no 3, p. 867-872Article in journal (Refereed)
  • 2.
    Ahlgren, Joakim
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Tranvik, Lars
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Evolution, Limnology.
    Gogoll, Adolf
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Biochemistry and Organic Chemistry, Organic Chemistry I.
    Waldebäck, Monica
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Markides, Karin
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Rydin, Emil
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Evolution, Limnology.
    Sediment Depth Attenuation of Biogenic Phosphorus Compounds Measured by 31P NMR2005In: Environmental Science and Technology, ISSN 0013-936X, E-ISSN 1520-5851, Vol. 39, no 3, p. 867-872Article in journal (Refereed)
    Abstract [en]

    Being a major cause of eutrophication and subsequent loss of water quality, the turnover of phosphorus (P) in lake sediments is in need of deeper understanding. A major part of the flux of P to eutrophic lake sediments is organically bound or of biogenic origin. This P is incorporated in a poorly described mixture of autochthonous and allochthonous sediment and forms the primary storage of P available for recycling to the water column, thus regulating lake trophic status. To identify and quantify biogenic sediment P and assess its lability, we analyzed sediment cores from Lake Erken, Sweden, using traditional P fractionation, and in parallel, NaOH extracts were analyzed using 31P NMR. The surface sediments contain orthophosphates (ortho-P) and pyrophosphates (pyro-P), as well as phosphate mono- and diesters. The first group of compounds to disappear with increased sediment depth is pyrophosphate, followed by a steady decline of the different ester compounds. Estimated half-life times of these compound groups are about 10 yr for pyrophosphate and 2 decades for mono- and diesters. Probably, these compounds will be mineralized to ortho-P and is thus potentially available for recycling to the water column, supporting further growth of phytoplankton. In conclusion, 31P NMR is a useful tool to asses the bioavailability of certain P compound groups, and the combination with traditional fractionation techniques makes quantification possible.

  • 3. Barnidge, David
    et al.
    Nilsson, Stefan
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Markides, Karin
    A design for low-flow sheathless electrospray emitters1999In: Analytical Chemistry, ISSN 0003-2700, Vol. 71, no 19, p. 4115-4118Article in journal (Refereed)
  • 4. Barnidge, David
    et al.
    Nilsson, Stefan
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Rapp, Håkan
    Hjort, Klas
    Markides, Karin
    Metallized sheathless electrospray emitters for use in capillary electrophoresis orthogonal time-of-flight mass spectrometry1999In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, Vol. 13, no 11, p. 994-1002Article in journal (Refereed)
  • 5. Hardenborg, Emilia
    et al.
    Zuberovic, Aida
    Ullsten, Sara
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Söderberg, Lennart
    Heldin, Eva
    Markides, Karin
    A novel polyamine coating providing non-covalent deactivation and reversed electroosmotic flow of fused-silica capillaries for capillary electrophoresis2003In: Journal of Chromatography A, Vol. 1003, no 1-2, p. 217-221Article in journal (Refereed)
  • 6.
    Hardenborg, Emilia
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Zuberovic, Aida
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Ullsten, Sara
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Söderberg, Lennart
    Surface Biotechnology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Physical and Analytical Chemistry.
    Heldin, Eva
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Markides, Karin
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Novel polyamine coating providing non-covalent deactivation and reversed electroosmotic flow of fused-silica capillaries for capillary electrophoresis2003In: J. of Chromatography A, no 1003, p. 217-221Article in journal (Refereed)
  • 7.
    Isaac, Giorgis
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Bylund, Dan
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Månsson, Jan-Eric
    Institute of Clinical Neuroscience, Sahlgrenska University Hospital, Göteborg University, Sweden.
    Markides, Karin
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
    Bergquist, Jonas
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
    Analysis of phosphatidylcholine and sphingomyelin molecular species from brain extracts using capillary liquid chromatography electrospray ionization mass spectrometry2003In: Journal of Neuroscience Methods, ISSN 0165-0270, E-ISSN 1872-678X, Vol. 128, no 1-2, p. 111-119Article in journal (Refereed)
    Abstract [en]

    One feature of complex lipids is that many subtypes of these molecules exist as a diverse mixture in a biological sample. Qualitative and quantitative analysis of these closely related molecules require sensitive and specific analytical methods to detect intact phospholipids (PL) and sphingomyelin (SM) species and to differentiate between them. Conventional analytical methods require laborious procedures including separation by column, argentation thin-layer chromatography or liquid chromatography (LC) after pre- or post-column derivatization. In the present work, a method based on reversed phase capillary LC coupled on-line to electrospray ionization mass spectrometry (LC/ESI/MS) has been developed to gather tools for lipidomic studies, i.e. the profiling of complex mixtures of lipids in small amounts of various cells and tissues. The LC/MS system used consisted of an LC pump in an isocratic elution, a reversed phase capillary column and a single quadrupole mass spectrometer operated in the positive ion mode. A successful separation of phosphatidylcholine (PC) and SM molecular species was obtained with a minimum detectable quantity (MDQ) in the low fmol range injected on column. The method was applied to human brain extracts. Furthermore, the extraction efficiencies of the traditional Folch method and pressurized fluid extraction (PFE) were compared using the human brain. It was found that the intensity of the PC and SM molecular species extracted by PFE is two times that of Folch.

  • 8.
    Lavén, Martin
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Appel, Lieuwe
    Moulder, Robert
    Tyrefors, Niklas
    Markides, Karin
    Långström, Bengt
    Determination of flumazenil in human plasma by liquid chromatography - electrospray ionisation - tandem mass spectrometry2004In: Journal of Chromatography B, Vol. 808, p. 221-227Article in journal (Refereed)
  • 9.
    Lavén, Martin
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Itsenko, Oleksiy
    Markides, Karin
    Långström, Bengt
    Determination of metabolic stability of positron emission tomography tracers by LC-ESI-MS/MS: an example in WAY-100635 and two analoguesIn: Journal of Pharmaceutical and Biomedical AnalysisArticle in journal (Refereed)
  • 10.
    Lavén, Martin
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Markides, Karin
    Långström, Bengt
    Analysis of microsomal metabolic stability using high-flow-rate extraction coupled to capillary liquid chromatography–mass spectrometry2004In: Journal of Chromatography B, Vol. 806, p. 119-126Article in journal (Refereed)
  • 11.
    Lavén, Martin
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry.
    Velikyan, Irina
    Djodjic, Majda
    Ljung, Jenny
    Berglund, Oskar
    Markides, Karin
    Långström, Bengt
    Wallenborg, Susanne
    Imaging of peptide adsorption to microfluidic channels in a plastic compact disc using a positron emitting radionuclideIn: Lab on a ChipArticle in journal (Refereed)
  • 12.
    Lavén, Martin
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Analytisk kemi.
    Velikyan, Irina
    Djodjic, Majda
    Ljung, Jenny
    Berglund, Oskar
    Markides, Karin
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Analytisk kemi.
    Långström, Bengt
    Wallenborg, Susanne
    Imaging of peptide adsorption to microfluidic channels in a plastic compact disc using a positron emitting radionuclide2005In: Lab Chip, no 5, p. 756-763Article in journal (Refereed)
    Abstract [en]

    A method for studying peptide-surface interactions within microfluidic channels by radionuclide imaging is described. With the high surface area-to-volume ratio of channels in miniaturised devices, combined with low amunts of analyte, non-specific peptide adsorption is a critical issue. The objective of the study was therefore to develop a method capable of direct detection of adsorbed peptide within microfluidic channels. A micro-device consisting of channels moulded in a plastic compact disc was chosen for the study, together with two selected peptides of different lengths and isolelectric point (pI) values. A bifunctional chelator, DOTA, was attached to the peptide by conjugation and labelled with the short-lived positron emitting radionuclide 68Ga. Quantitative images of radiotracer distribution within the microfluidic channels were obtained using a PhosphorImager system. The power of the method was demonstrated by the ability to clearly measure changes in adsorption when varying a number of parameters that typically affect peptide adsorption. These included surface modifications, analyte concentration, pH, and ionic strength. Additionally, two quantification methods were developed and compared. Radionuclide imaging also permitted visualisation of adsorption and release processes in microchannel chromatographic columns. The results suggest that radionuclide imaging is a suitable tool not only for the study of peptide adsorption to the microchannels presented in this study but also as a versatile tool to measure peptide-surface interactions in a wide variety of miniaturised structures and devices.

  • 13. Nilsson, Marie-Louise
    et al.
    Waldebäck, Monica
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Liljegren, Gustav
    Kylin, Henrik
    Markides, Karin
    Pressurized Fluid Extraction (PFE) of Chlorinated Paraffins from the Biodegradable Fraction of Source-Separated Household Waste2001In: Fresenius Journal of Analytical Chemistry, Vol. 370, p. 913-918Article in journal (Refereed)
  • 14.
    Nilsson, Stefan
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Markides, Karin
    On-column conductive coating for thermolabile columns used in capillary zone electrophoresis sheathless electrospray ionization mass spectrometry2000In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, Vol. 14, no 1, p. 6-11Article in journal (Refereed)
  • 15.
    Nilsson, Stefan
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Svedberg, Malin
    Pettersson, Jean
    Björefors, Fredrik
    Markides, Karin
    Nyholm, Leif
    Evaluations of the stability of sheathless electrospray ionization mass spectrometry emitters using electrochemical techniques2001In: Analytical Chemistry, ISSN 0003-2700, Vol. 73, no 19, p. 4607-4616Article in journal (Refereed)
  • 16.
    Nilsson, Stefan
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Wetterhall, Magnus
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Bergquist, Jonas
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Nyholm, Leif
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Markides, Karin
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    A simple and robust conductive graphite coating for sheathless electrospray emitters used in capillary electrophoresis/mass spectrometry2001In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 15, no 21, p. 1997-2000Article in journal (Refereed)
    Abstract [en]

    A graphite-polyimide mixture was used as a conductive coating for sheathless electrospray emitters. The coating procedure described is simple and inexpensive compared to previously described methods. An investigation of the stability of the conductive coating carried out by electrochemical methods revealed good performances during oxidative stress. In addition, no decrease in emitter performance was seen during continuous electrospray in the positive electrospray mode for two weeks. Fast capillary electrophoresis with attomole sensitivity demonstrated the excellent performance of the described sheathless interface when used in conjunction with an orthogonal time-of-flight mass spectrometer. The overall simplicity, stability and low cost of this type of sheathless emitter make the described approach highly suitable for any on-column coupling of low flow rate separation techniques to a mass spectrometer.

  • 17. Pihlström, Tuija
    et al.
    Isaac, Giorgis
    Waldebäck, Monica
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Österdahl, Bengt-Göran
    Markides, Karin
    Pressurised Fluid Extraction (PFE) as an Alternative General Method for Analysis of Pesticide Residues in Rape Seed2002In: The Analyst, Vol. 127, p. 554-559Article in journal (Refereed)
  • 18.
    Ramström, Margareta
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Hagman, Charlotte
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, Ion Physics.
    Tsybin, Youri O
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, Ion Physics.
    Markides, Karin
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
    Håkansson, Per
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, Ion Physics.
    Salehi, Albert
    Department of Pharmacology, Institute of Physiological Sciences, Lund University.
    Lundquist, Ingmar
    Department of Pharmacology, Institute of Physiological Sciences, Lund University.
    Håkanson, Rolf
    Department of Pharmacology, Institute of Physiological Sciences, Lund University.
    Bergquist, Jonas
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
    A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets2003In: European Journal of Biochemistry, ISSN 0014-2956, E-ISSN 1432-1033, Vol. 270, no 15, p. 3146-3152Article in journal (Refereed)
    Abstract [en]

    Liquid chromatography mass spectrometry (LC-MS) is a valuable tool in the analysis of proteins and peptides. The combination of LC-MS with different fragmentation methods provides sequence information on components in complex mixtures. In this work, on-line packed capillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation techniques, i.e. nozzle-skimmer fragmentation and electron capture dissociation, for the determination of hormonal peptides in an acid ethanol extract of mouse pancreatic islets. The most abundant peptides, those derived from proinsulin and proglucagon, were identified by their masses and additional sequence-tag information established their identities. Interestingly, the experiments demonstrated the presence of truncated C-peptides, des-(25-29)-C-peptide and des-(27-31)-C-peptide. These novel findings clearly illustrate the potential usefulness of the described technique for on-line sequencing and characterization of peptides in tissue extracts.

  • 19. Selditz, Ulrike
    et al.
    Nilsson, Stefan
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Barnidge, David
    Markides, Karin
    MS detection for microseparation techniques1999In: Chimia, ISSN 0009-4293, Vol. 53, no 10, p. 506-510Article in journal (Refereed)
  • 20. Svedberg, Malin
    et al.
    Pettersson, Andreas
    Nilsson, Stefan
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry.
    Bergquist, Jonas
    Nyholm, Leif
    Nikolajeff, Fredrik
    Markides, Karin
    Sheathless electrospray from polymer microchips2003In: Analytical Chemistry, ISSN 0003-2700, Vol. 75, no 15, p. 3934-3940Article in journal (Refereed)
  • 21.
    Thorslund, Sara
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
    Bergström, S
    Johanesson, N
    Pettersson, A
    Liljegren, G
    Magnusson, K
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
    Hallberg, M
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
    Klett, Oliver
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
    Bergquist,, Jonas
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Nyholm, Leif
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry.
    Markides, Karin
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry, Inorganic Chemistry. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Materials Science.
    Nyberg, F
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry, Inorganic Chemistry. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Materials Science.
    Nikolajeff, Fredrik
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
    Essential components in an integrated platform for on-site screening of anabolic androgenic steroids in biological samples2004In: Micro Total Analysis Systems 2004: Vol 1 / [ed] Laurell T; Nilsson J; Jensen K; Harrison DJ; Kutter JP, 2004Conference paper (Refereed)
    Abstract [en]

    In this abstract new results concerning the introduction of a biological sample into a mu TAS, the avoidance of unspecific absorbance in the mu TAS and the interfacing to off-chip detection (e.g. Mass spectrometry, MS) for verification are presented.

  • 22.
    Ullsten, Sara
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Söderberg, Lennart
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Folestad, Staffan
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Markides, Karin
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Quaternary ammonium substituted agarose as surface coating in capillary electrophoresis2004In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 129, p. 410-415Article in journal (Refereed)
    Abstract [en]

    A novel positively charged polymer of quaternary ammonium substituted agarose (Q-agarose) has been synthesized and explored for use as a coating in capillary electrophoresis. The fast and simple coating procedure is based on a multi-site electrostatic interaction between the polycationic agarose polymer and the negatively charged fused-silica surface. By simply flushing fused-silica capillaries with hot polymer solution a positively charged, hydrophilic deactivation layer is achieved. The polymer surface provides an intermediate electroosmotic flow of reversed direction, over a range of pH 2-11, compared to unmodified fused-silica. The coating procedure was highly reproducible with an RSD of 4%, evaluated as the electroosmotic flow mobility for 30 capillaries prepared at 10 different occasions. The application of Q-agarose coated capillaries in separation science was investigated using a set of basic drugs and model proteins and peptides. Due to the intermediate electroosmotic flow generated, the resolution of basic drugs could be increased, compared to using bare fused-silica capillaries. Moreover, the coating enabled separation of proteins and peptides with efficiencies up to 300.000 plates m(-1).

  • 23. Ullsten, Sara
    et al.
    Örnskov, Eivor
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Sjöberg, Per
    Edwards, Katarina
    Karlsson, Göran
    Markides, Karin
    Folestad, Staffan
    Immobilised-Liposome Capillary Electrophoresis - On-line Mass Spectrometry for Biopharmaceutical Drug ProfilingManuscript (Other academic)
  • 24.
    Ullsten, Sara
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry.
    Örnskov, Eivor
    Sjöberg, Per
    Edwards, Katarina
    Karlsson, Göran
    Markides, Karin
    Folestad, Staffan
    Immobilized-lipoosme capillary electrophoresis-on-line mass psectrometry for biopharmaceutical drug profilingManuscript (Other academic)
  • 25.
    Waldeback, Monica
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Physical and Analytical Chemistry, Analytical Chemistry. Faculty of Science and Technology, Biology, Department of Ecology and Evolution, Limnology.
    Rydin, Emil
    Department of Evolutionary Biology. Department of Physical and Analytical Chemistry, Analytical Chemistry. Faculty of Science and Technology, Biology, Department of Ecology and Evolution, Limnology.
    Markides, Karin
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Physical and Analytical Chemistry, Analytical Chemistry. Faculty of Science and Technology, Biology, Department of Ecology and Evolution, Limnology.
    Use of accelerated solvent extraction for determination of ecological important phosphorus in lake sediments1998In: INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, ISSN 0306-7319, Vol. 72, no 4, p. 257-266Article in journal (Refereed)
    Abstract [en]

    Phosphorus has been identified as one of the most important elements in eutrophication of lakes. and the bulk of phosphorus compounds stored in lake sediment contribute to a large extent to this process. It is therefore of great interest to get an adequa

  • 26.
    Waldebäck, Monica
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Jansson, Charlotte
    Senorans, Javier
    Markides, Karin
    Accelerated Solvent Extraction of the Antioxidant Irganox 1076 in Linear Low Density Polyethylene (LLDPE)-Granules Before and After γ-Irradiation1998In: The Analyst, Vol. 123, no 6, p. 1205-1207Article in journal (Refereed)
  • 27.
    Waldebäck, Monica
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Rydin, Emil
    Markides, Karin
    Use of Accelerated Solvent Extraction for Determination of Ecological Important Phosphorus in Lake Sediment1998In: International Journal of Environmental Analytical Chemistry, Vol. 72, p. 257-266Article in journal (Refereed)
  • 28.
    Wetterhall, Magnus
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Nilsson, Stefan
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Markides, Karin
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Bergquist, Jonas
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    A conductive polymeric material used for nanospray needle and low-flow sheathless electrospray ionization applications2002In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 74, no 1, p. 239-245Article in journal (Refereed)
    Abstract [en]

    A conductive polypropylene/graphite mixture is used for the production of polymeric nanospray needle emitters and as a coating on fused-silica capillaries that are used for sheathless electrospray ionization (ESI). The described production of these polymeric nanospray needle emitters and sheathless ESI contacts is exceptionally easy and at a very low cost. The described polymeric nanospray emitters have shown excellent features regarding their chemical inertness and spray performance. The long-term stability of the nanospray needles exceeds 24 h of continuous use. Furthermore, the resistance to electrical discharges, which is one of the factors that often limits the lifetime of metal coated tips, has proven to be outstanding. A voltage of up to 5 kV could be applied without loss of spray performance. The use of polypropylene emitters offers a number of desirable features, as compared to silica based emitters. Among these features are mechanical flexibility and simplified regeneration of the nanospray needle. Continuous nanospray of peptides and proteins in conjunction with orthogonal time-of-flight mass spectrometry are shown with signal relative standard deviations of 5%. In addition, the polypropylene/graphite mixture has also been applied as the conductive contact for sheathless ESI in fast capillary electrophoresis separations.

  • 29. Zuberovic, Aida
    et al.
    Ullsten, Sara
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry.
    Hellman, Ulf
    Markides, Karin
    Bergquist, Jonas
    Capillary electrophoresis-off-line-matrix-assisted laser desorption/ionisation mass spectrometry of proteins and peptides using cationic-coated capillariesManuscript (Other academic)
  • 30. Örnskov, Eivor
    et al.
    Ullsten, Sara
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Söderberg, Lennart
    Markides, Karin
    Folestad, Staffan
    Method for immobilization of liposomes in capillary electrophoresis2002In: Electrophoresis, Vol. 23, no 19, p. 3381-3384Article in journal (Refereed)
  • 31.
    Örnskov, Eivor
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry, Analytical Chemistry.
    Ullsten, Sara
    Söderberg, Lennart
    Markides, Karin
    Folestad, Staffan
    Method for Immobilization of Liposomes in Capillary Electrophoresis by Electrostatic Interaction with Derivatized Agarose2002In: Electrophoresis, Vol. 23, no 19, p. 3381-3384Article in journal (Refereed)
  • 32.
    Örnskov, Eivor
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Ullsten, Sara
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Söderberg, Lennart
    Surface Biotechnology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Physical and Analytical Chemistry.
    Markides, Karin
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Folestad, Staffan
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
    Method for immobilization of liposomes in capillary electrophoresis by electrostatic interaction with derivatized agarose2002In: Electrophoresis, ISSN 3381-3384, Vol. 23, p. 3381-3384Article in journal (Refereed)
    Abstract [en]

    A novel procedure for immobilization of liposomes inside fused-silica capillaries is

    demonstrated. First, the inner wall of the capillaries was coated with a positively

    charged polymer, composed of derivatized agarose. Subsequently, negatively charged

    liposomes were immobilized by electrostatic interaction on the polymer coating. The

    developed liposome coated capillaries were used as a nanoseparation tool for studying

    interactions between small drug compounds and liposomes. Part of this work was

    presented at the 15th International Symposium on Microscale Separations and Analysis,

    HPCE 2002, Stockholm, Sweden, April 2002.

1 - 32 of 32
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