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  • 1.
    Adler, Jeremy
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
    Colour blindness: journals should enable image redisplay2021In: Nature, ISSN 0028-0836, E-ISSN 1476-4687, Vol. 599, no 7883, p. 32-32Article in journal (Other academic)
  • 2.
    Adrian, Monica Lovestam
    et al.
    Lund Univ, Dept Ophthalmol, SE-22185 Lund, Sweden.
    Vassilev, Zdravko P.
    Bayer US, Whippany, NJ USA.
    Westborg, Inger
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Baseline visual acuity as a prognostic factor for visual outcomes in patients treated with aflibercept for wet age-related macular degeneration: data from the INSIGHT study using the Swedish Macula Register2019In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 97, no 1, p. 91-98Article in journal (Refereed)
    Abstract [en]

    Purpose: To assess mean change in visual acuity (VA) overall and stratified by baseline VA after 1 and 2 years' treatment with aflibercept in a real-life setting.

    Methods: This was an observational cohort study using nationwide data from the Swedish Macula Register. Treatment-naive patient eyes with wet age-related macular degeneration and prescribed aflibercept from January 2013 to December 2014 were followed for 1 year (2478 eyes) or 2 years (831 eyes) to assess VA. Eyes were grouped by baseline VA.

    Results: Mean number of injections in patients treated according to label (72%) versus patients treated not according to label was 8.0 +/- 1.5 versus 4.4 +/- 0.8 (p < 0.0001) at 1 year, and 12.5 +/- 3.2 versus 7.3 +/- 1.9 (p < 0.0001) at 2 years. Among all eyes, mean VA increased from 61.3 +/- 13.4 Early Treatment Diabetic Retinopathy Study letters at baseline to 64.5 +/- 15.6 at 1 year and 65.1 +/- 15.1 letters at 2 years. At 2 years, eyes with good baseline vision (>= 70 letters) lost a mean of 2.4 +/- 11.3 to 72.3 letters, eyes with intermediate baseline VA (36-69 letters) gained 5.7 +/- 14.1 to 62.7 letters, and eyes with poor baseline VA (<= 35 letters) gained 13.2 +/- 18.3 to 41.0 letters. Also at 2 years, 75% of treated eyes were stable or had improved VA. Among eyes with intermediate baseline VA, near vision was significantly better among those treated according to label versus not according to label at 3 (p = 0.019), 6 (p = 0.0002) and 12 months (p <= 0.0001).

    Conclusion: While gain in vision was especially pronounced in eyes with poor baseline VA, good baseline VA was important for best prognosis.

  • 3.
    Alm, A.
    et al.
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience. Oftalmiatrik.
    Schoenfelder, J.
    McDermott, J.
    A 5-year, multicenter, open-label, safety study of adjunctive latanoprost therapy for glaucoma.2004In: Arch Ophthalmol, Vol. 122, p. 957-965Article in journal (Refereed)
  • 4.
    Alm, Albert
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Wikström, Carl Peter
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Ekström, Curt
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Öhman, Lena
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    The effect of metoprolol on intra-ocular pressure in glaucoma. A pilot study.1979In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 57, no 2, p. 236-242, article id j.1755-3768.1979.tb00487.xArticle in journal (Refereed)
  • 5.
    An, Hongbin
    et al.
    HUST, Sch Mech Sci & Engn, Wuhan 430074, Hubei, Peoples R China.
    Chen, Liangzhou
    HUST, Sch Mech Sci & Engn, Wuhan 430074, Hubei, Peoples R China.
    Liu, Xiaojun
    HUST, Sch Mech Sci & Engn, Wuhan 430074, Hubei, Peoples R China.
    Zhao, Bin
    HUST, Sch Mech Sci & Engn, Wuhan 430074, Hubei, Peoples R China.
    Zhang, Hong
    HUST, Tongji Med Coll, Tongji Hosp, Dept Ophthalmol, Wuhan 430074, Hubei, Peoples R China.
    Wu, Zhigang
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. HUST, Sch Mech Sci & Engn, Wuhan 430074, Hubei, Peoples R China.
    Microfluidic contact lenses for unpowered, continuous and non-invasive intraocular pressure monitoring2019In: Sensors and Actuators A-Physical, ISSN 0924-4247, E-ISSN 1873-3069, Vol. 295, p. 177-187Article in journal (Refereed)
    Abstract [en]

    Intraocular pressure (IOP) is a crucial physiological indicator of the visual system and play a key role in the diagnosis and treatment of glaucoma. However, the current handheld single measurement tools for IOP sensing cannot meet the future demands for glaucoma management. Thus, here we present the microfluidic contact lens sensors that could provide unpowered, continuous and non-invasive IOP monitoring. The microfluidic contact lens is comprised of a sensing layer of the micropatterned soft-elastomer and a hard plastic reference layer. The devices use the annular sensing chamber filled with the dyed liquid and a sensing microchannel as the IOP transducer. Resulting from the volume variance of the sensing chamber and caused by the deformation of the sensing layer under pressure, the IOP signal is detected as the displacement change of the dyed liquid's interface in the sensing channel, and in which, the displacement change can be optically observed by using the smart-phone camera. Based on the silicone rubber model eyeball, the sensing mechanism of the devices with different design parameters (the position of the sensing chambers and the dimension of the sensing channels) are explored by using the theoretical analyses and experimental investigations. The characteristics of these microfluidic contact lens sensors are tested, in which, the maximum sensitivity of the device (with the sensing chamber of 8.5 mm in diameter and the sensing channel of 100 x 40 um in size) can be achieved to 0.708 mm/mmHg in a working range of 0 (4) over tilde0 mmHg. Also, cyclical tests were conducted and indicated that the devices had a good reversibility and Long-term stability. Furthermore, the device (with the sensing chamber of 5.0 mm in diameter and the sensing channel of 150 x 40 urn in size) was test on the porcine eyes ex vivo, showing a sensitivity of 0.2832 mm/mmHg in a range of 8 (3) over tilde2 mmHg and, the device had a good reproducibility to its IOP change. This work provides a promising approach for unpowered, continuous and non-invasive monitoring of IOP.

  • 6.
    Aurell, Sandra
    et al.
    Vastmanland Cty Hosp, AT Ctr, S-72189 Vasteras, Sweden..
    Granstam, Elisabet
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Centre for Clinical Research, County of Västmanland. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology. Vastmanland Cty Hosp, Dept Ophthalmol, Vasteras, Sweden..
    Bacterial keratitis in a Swedish county hospital: management and clinical outcome2016In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 94, no 3, p. E248-E249Article in journal (Refereed)
  • 7.
    Aurell, Sandra
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Centre for Clinical Research, County of Västmanland. Hosp Vastmanland, Dept Ophthalmol, Vasteras, Sweden.
    Sjövall, Kersti
    Hosp Vastmanland, Dept Ophthalmol, Vasteras, Sweden.
    Paul, Anna
    Hosp Vastmanland, Dept Ophthalmol, Vasteras, Sweden.
    Morén, Åsa
    Hosp Vastmanland, Dept Ophthalmol, Vasteras, Sweden.
    Granstam, Elisabet
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Centre for Clinical Research, County of Västmanland. Hosp Vastmanland, Dept Ophthalmol, Vasteras, Sweden.
    Better visual outcome at 1 year with antivascular endothelial growth factor treatment according to treat-and-extend compared with pro re nata in eyes with neovascular age-related macular degeneration2019In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 97, no 5, p. 519-524Article in journal (Refereed)
    Abstract [en]

    Purpose: To evaluate treatment outcome at 12 months in eyes with neovascular age-related macular degeneration (nAMD) treated with antivascular endothelial growth factor (anti-VEGF) injections according to either pro re nata (PRN)- or treat-and-extend (TE)-regimen in one clinical setting in Sweden.

    Methods: Data were obtained retrospectively from the Swedish Macula Register, optical coherence tomography-database and electronic patient charts. The study included 443 eyes; 223 PRN- and 220 TE-treated eyes. Baseline (BL) characteristics and follow-up data at 6 and 12 months were collected. Statistical regression analysis was performed to evaluate association between treatment strategy and visual outcome at 12 months.

    Results: Baseline (BL) characteristics were well balanced between cohorts. Visual acuity at 12 months was higher in TE-cohort 66.5 (13.1) compared to PRN-cohort 60.1 (17.6) (p = 0.000). Visual improvement at 12 months was +5.2 (11.8) and +1.2 (12.7) letters Early Treatment Diabetic Retinopathy Study (ETDRS) in TE- and PRN-cohorts, respectively (p = 0.002). Number of administered injections at 12 months was 10.2 (2.1) and 6.3 (2.1) in the two cohorts (p = 0.000). Statistical analysis demonstrated a strong association between TE treatment strategy and improvement in visual acuity at 12 months.

    Conclusion: Eyes treated according to TE had better visual outcome at 12 months. The results indicate that treatment according to proactive TE-regimen is superior to treatment according to PRN-regimen in clinical routine care of nAMD.

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  • 8.
    Austeng, Dordi
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Retinopathy of Prematurity in Infants Born Before 27 Weeks of Gestation: A National Population-based Study in Sweden During 2004-20072010Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Background: Improved neonatal care has resulted in an increasing population of surviving infants. Neonatal morbidity in preterm infants is, however, high, and retinopathy of prematurity (ROP) is one of the major neonatal morbidities. Observations have suggested that ROP might have a different course in extremely preterm compared to more mature infants.

    Aims: To study the incidence, natural history and treatment of the disease, and the implications regarding screening recommendations for the population of extremely preterm infants.

    Methods: A national, population-based study of neonatal morbidity in infants born before 27 gestational weeks was performed in Sweden during 2004 to 2007. ROP screening started in the 5th postnatal week and continued until the retina was completely vascularized.

    Results: Of the 506 infants surviving until the first ROP examination, 73% developed ROP; 38% mild ROP and 35% severe ROP. Ninety-nine infants (20%) were treated. A log-linear relationship was found between severe ROP and gestational age (GA) at birth, and the risk of ROP was reduced by 50% for each week of increase in GA at birth (Paper I).

    Postmenstrual age (PMA) at onset of ROP was significantly related to GA at birth, as was the site of onset of ROP. ROP had a predilection to start in the nasal retina in the most immature infants. There were significant relations between PMA at onset of ROP and severity of ROP as well as between the site of onset of ROP and severe ROP (Paper III).

    The most immature infants had a higher risk of reaching treatment criteria for ROP, a higher risk of progression from ROP 3 to treatment criteria, and they reached these criteria at an earlier PMA than the less immature infants (Paper II).

    According to our results, the first examination can be postponed until a PMA of 31 weeks in infants born before 27 weeks of gestation, since onset of ROP 3 did not occur before this age, and criteria for treatment were not reached before 32 weeks. The majority of infants (75%) were treated during a limited period, i.e. before a PMA of 39 weeks (Paper IV).

    List of papers
    1. Incidence of retinopathy of prematurity in infants born before 27 weeks' gestation in Sweden
    Open this publication in new window or tab >>Incidence of retinopathy of prematurity in infants born before 27 weeks' gestation in Sweden
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    2009 (English)In: Archives of ophthalmology (1960), ISSN 0003-9950, Vol. 127, no 10, p. 1315-1319Article in journal (Refereed) Published
    Abstract [en]

    OBJECTIVE: To determine the incidence of retinopathy of prematurity (ROP) in extremely preterm infants born before 27 weeks' gestation in Sweden during a 3-year period. METHODS: A national, prospective, population-based study was performed in Sweden from April 1, 2004, to March 31, 2007. The ophthalmologic part of the study was separately organized, and screening for ROP was performed beginning postnatal week 5. The criteria for the treatment of ROP agreed with the recommendations of the Early Treatment for Retinopathy of Prematurity Cooperative Group. RESULTS: During the study, 506 of 707 live-born infants survived until the first eye examination. Of these, 368 (72.7%) had ROP: 37.9% had mild ROP and 34.8% had severe ROP. Ninety-nine infants (19.6%) were treated. Gestational age at birth was a stronger predictor of ROP than was birth weight. A log-linear relationship between severe ROP and gestational age at birth was found in the present cohort, and the risk of ROP was reduced by 50% for each week of increase in gestational age at birth. CONCLUSIONS: Today, extremely preterm infants are surviving, and this population-based study with ROP as a primary outcome shows a higher incidence of this condition than in previously reported national cohorts.

    National Category
    Medical and Health Sciences
    Identifiers
    urn:nbn:se:uu:diva-121732 (URN)10.1001/archophthalmol.2009.244 (DOI)000287329500008 ()19822848 (PubMedID)
    Available from: 2010-03-29 Created: 2010-03-29 Last updated: 2017-12-12Bibliographically approved
    2. Treatment for retinopathy of prematurity in infants born before 27 weeks of gestation in Sweden
    Open this publication in new window or tab >>Treatment for retinopathy of prematurity in infants born before 27 weeks of gestation in Sweden
    Show others...
    2010 (English)In: British Journal of Ophthalmology, ISSN 0007-1161, E-ISSN 1468-2079, Vol. 94, no 9, p. 1136-1139Article in journal (Refereed) Published
    Abstract [en]

    AIMS: To study various aspects of treatment for retinopathy of prematurity (ROP) in a Swedish population of extremely preterm infants born before 27 weeks of gestation. METHODS: A national, prospective and population-based study was performed in Sweden from April 1, 2004 to March 31, 2007. The criteria for treatment of ROP accorded with the recommendations of the Early Treatment for Retinopathy of Prematurity Cooperative Group. RESULTS: Twenty percent of the infants (99/506) were treated for ROP. The likelihood of reaching treatment criteria nearly doubled for each week of reduction in gestational age (GA) at birth. The first treatment was performed at an earlier postmenstrual age in the most immature infants. One third of the infants had more than one session of laser treatment. CONCLUSIONS: A high percentage of these extremely preterm infants required treatment for ROP. The likelihood of reaching treatment criteria increased with a decline in GA at birth. Although only a few infants progressed to ROP Stages 4 and 5, our findings indicate a potential for improvement of the treatment routines, both regarding timing and number of laser spots at the first treatment.

    Keywords
    Retinopathy of prematurity, extremely preterm, population-based, treatment
    National Category
    Ophthalmology
    Research subject
    Ophtalmology; Pediatrics
    Identifiers
    urn:nbn:se:uu:diva-121731 (URN)10.1136/bjo.2009.170704 (DOI)000282206500006 ()19951941 (PubMedID)
    Available from: 2010-03-29 Created: 2010-03-29 Last updated: 2022-01-28Bibliographically approved
    3. Natural history of retinopathy of prematurity in infants born before 27 weeks' gestation in Sweden
    Open this publication in new window or tab >>Natural history of retinopathy of prematurity in infants born before 27 weeks' gestation in Sweden
    Show others...
    2010 (English)In: Archives of ophthalmology (1960), ISSN 0003-9950, Vol. 128, no 10, p. 1289-1294Article in journal (Refereed) Published
    Abstract [en]

    Objective: To investigate the natural history of retinopathy of prematurity (ROP) in 506 extremely preterm infants born before 27 weeks' gestation in Sweden during a 3-year period.

    Methods: A national population–based study was performed in Sweden from April 1, 2004, to March 31, 2007. According to the study protocol, initial eye examinations were to be performed at postnatal week 5, and examinations were repeated until the retina was completely vascularized or until criteria for treatment were met. The examinations were to be performed weekly, enabling study of the course and severity of ROP. In infants without ROP or with mild ROP without progression during the latest examinations, further examinations were performed weekly or every other week from postmenstrual age 35 weeks.

    Results: During the study, 368 infants (72.7%) developed ROP. Postmenstrual age at onset of ROP was significantly related to severity of ROP, even when controlling for gestational age (ie, the earlier the onset of ROP, the higher the risk of developing severe ROP). Site of onset of ROP was significantly related to gestational age at birth. The risk of nasal onset was almost doubled for every week of decrease in gestational age at birth. Nasal onset was associated with severe ROP, even after adjusting for gestational age at birth.

    Conclusion: This population-based study confirms results of the Cryotherapy for Retinopathy of Prematurity study and shows new correlations regarding time and site of onset of ROP, both of which are associated with disease severity.

    Place, publisher, year, edition, pages
    American Medical Association, 2010
    Keywords
    Retinopathy of prematurity, extremely preterm, population-based, natural history
    National Category
    Ophthalmology
    Research subject
    Ophtalmology
    Identifiers
    urn:nbn:se:uu:diva-122983 (URN)10.1001/archophthalmol.2010.234 (DOI)000282844000007 ()
    Available from: 2010-04-22 Created: 2010-04-22 Last updated: 2022-01-28Bibliographically approved
    4.
    The record could not be found. The reason may be that the record is no longer available or you may have typed in a wrong id in the address field.
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    FULLTEXT01
  • 9.
    Austeng, Dordi
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Källen, Karin B. M.
    Lund University.
    Ewald, Uwe W.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health.
    Wallin, Agneta
    St.Erik's Eye Hospital.
    Holmström, Gerd E.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Treatment for retinopathy of prematurity in infants born before 27 weeks of gestation in Sweden2010In: British Journal of Ophthalmology, ISSN 0007-1161, E-ISSN 1468-2079, Vol. 94, no 9, p. 1136-1139Article in journal (Refereed)
    Abstract [en]

    AIMS: To study various aspects of treatment for retinopathy of prematurity (ROP) in a Swedish population of extremely preterm infants born before 27 weeks of gestation. METHODS: A national, prospective and population-based study was performed in Sweden from April 1, 2004 to March 31, 2007. The criteria for treatment of ROP accorded with the recommendations of the Early Treatment for Retinopathy of Prematurity Cooperative Group. RESULTS: Twenty percent of the infants (99/506) were treated for ROP. The likelihood of reaching treatment criteria nearly doubled for each week of reduction in gestational age (GA) at birth. The first treatment was performed at an earlier postmenstrual age in the most immature infants. One third of the infants had more than one session of laser treatment. CONCLUSIONS: A high percentage of these extremely preterm infants required treatment for ROP. The likelihood of reaching treatment criteria increased with a decline in GA at birth. Although only a few infants progressed to ROP Stages 4 and 5, our findings indicate a potential for improvement of the treatment routines, both regarding timing and number of laser spots at the first treatment.

    Download full text (pdf)
    FULLTEXT01
  • 10.
    Austeng, Dordi
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Källen, Karin
    Hellström, Ann
    Jakobsson, Peter
    Lundgren, Pia
    Tornqvist, Kristina
    Wallin, Agneta
    Holmström, Gerd
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Regional differences in screening for retinopathy of prematurity in infants born before 27 weeks' gestation in Sweden: the EXPRESS study2014In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 92, no 4, p. 311-315Article in journal (Refereed)
    Abstract [en]

    Purpose:  The primary aim was to analyse regional incidences of retinopathy of prematurity (ROP) and frequencies of treatment and their relation to perinatal risk factors during a 3-year period. A secondary aim was to study adherence to the study screening protocol in the different regions.

    Methods:  A population-based study of neonatal morbidity in extremely preterm infants in Sweden (EXPRESS) was performed during 2004–2007. Screening for ROP was to start at postnatal age 5 weeks and to continue weekly until the retina was completely vascularized or until regression of ROP. Logistic regression analyses were used for evaluation of differences in incidence of Any ROP, ROP 3 or more and ROP Type 1 between the seven regions of the country.

    Results:  The regional incidence of ROP varied between 54% and 92% for Any ROP, between 25% and 43% for ROP stage 3 or more and between 8% and 23% of infants with ROP Type 1, all of whom were treated. There was no significant difference between the regions regarding ROP Type 1, even when adjusting for known risk factors for ROP.

    Conclusion:  The heterogeneity between the regions regarding the incidence of ROP was reduced with increasing severity of ROP, and there was no heterogeneity regarding frequency of treatment for ROP, which is the most important issue for the children. We cannot exclude observer bias regarding mild ROP and ROP stage 3 in this study.

  • 11.
    Austeng, Dordi
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Källen, Karin
    Tornbladsinstitutet, Lund University.
    Hellström, Ann
    Section of Pediatric Ophthalmology, The Queen Silvia Children's Hospital, Sahlgrenska Academy, University of Gothenburg .
    Tornqvist, Kristina
    Department of Ophthalmology, Lund University Hospital.
    Holmström, Gerd E.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Natural history of retinopathy of prematurity in infants born before 27 weeks' gestation in Sweden2010In: Archives of ophthalmology (1960), ISSN 0003-9950, Vol. 128, no 10, p. 1289-1294Article in journal (Refereed)
    Abstract [en]

    Objective: To investigate the natural history of retinopathy of prematurity (ROP) in 506 extremely preterm infants born before 27 weeks' gestation in Sweden during a 3-year period.

    Methods: A national population–based study was performed in Sweden from April 1, 2004, to March 31, 2007. According to the study protocol, initial eye examinations were to be performed at postnatal week 5, and examinations were repeated until the retina was completely vascularized or until criteria for treatment were met. The examinations were to be performed weekly, enabling study of the course and severity of ROP. In infants without ROP or with mild ROP without progression during the latest examinations, further examinations were performed weekly or every other week from postmenstrual age 35 weeks.

    Results: During the study, 368 infants (72.7%) developed ROP. Postmenstrual age at onset of ROP was significantly related to severity of ROP, even when controlling for gestational age (ie, the earlier the onset of ROP, the higher the risk of developing severe ROP). Site of onset of ROP was significantly related to gestational age at birth. The risk of nasal onset was almost doubled for every week of decrease in gestational age at birth. Nasal onset was associated with severe ROP, even after adjusting for gestational age at birth.

    Conclusion: This population-based study confirms results of the Cryotherapy for Retinopathy of Prematurity study and shows new correlations regarding time and site of onset of ROP, both of which are associated with disease severity.

  • 12. Ayala, Marcelo N.
    et al.
    Söderberg, Per G.
    St. Erik’s Eye Hospital, Karolinska Institutet, Stockholm, Sweden.
    Reversal of reciprocity failure for UVR-induced cataract with vitamin E2005In: Ophthalmic Research, ISSN 0030-3747, E-ISSN 1423-0259, Vol. 37, no 3, p. 150-155Article in journal (Refereed)
    Abstract [en]

    PURPOSE: The authors have previously described that the photochemical reciprocity law does not apply for ultraviolet radiation (UVR)-induced cataract. The aim of this study was to elucidate if failure of the reciprocity reverses with vitamin E (alpha-tocopherol) administration. METHODS: Altogether, 80 rats were divided into one group fed alpha-tocopherol and one control group. For each group, half of rats were exposed to UVR for 5 min and the remaining rats for 15 min. RESULTS: Lenses exposed to UVR for 5 min showed no difference in light scattering between alpha-tocopherol-treated and untreated groups. Lenses exposed to UVR for 15 min showed significant difference in light scattering between alpha-tocopherol-treated and untreated groups. CONCLUSIONS: Failure in exposure time-intensity reciprocity for UVR-induced cataract with exposures shorter than 30 min may be due to consumption of antioxidants in the lens.

  • 13.
    Bartuma, Katarina
    et al.
    Karolinska Inst, St Erik Eye Hosp, Dept Vitreoretinal Dis, Stockholm, Sweden..
    Pal, Niklas
    Karolinska Inst, Dept Pediat, Astrid Lindgren Childrens Hosp, S-10401 Stockholm, Sweden..
    Kosek, Sonja
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. Uppsala Univ, Uppsala, Sweden..
    Holm, Stefan
    Karolinska Inst, Dept Pediat, Astrid Lindgren Childrens Hosp, S-10401 Stockholm, Sweden..
    All-Ericsson, Charlotta
    Karolinska Inst, St Erik Eye Hosp, Dept Vitreoretinal Dis, Stockholm, Sweden..
    A 10-year experience of outcome in chemotherapy-treated hereditary retinoblastoma2014In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 92, no 5, p. 404-411Article in journal (Refereed)
    Abstract [en]

    Purpose: The aim is to report the 10-year retrospective experience of systemic chemotherapy for a population-based group of patients with hereditary retinoblastoma at a national referral centre. The outcomes include control rates, treatment side-effects, adjuvant therapy, failure rate, survival, secondary cancers and visual acuity. Methods: All patients (n = 24, 46 eyes) diagnosed with retinoblastoma and treated with systemic chemotherapy at a national referral centre during 2001-2011 were included. Data were extracted from medical records. Results: The patients were followed for a mean of 60 months (range 13-144). Four-six cycles of VEC was administered to all newly diagnosed group B/C/D/E eyes with bilateral disease and 83% (38 of 46) responded to the treatment. None of the patients discontinued chemotherapy because of adverse reactions. Altogether 26% (12 of 46) of the eyes received second-line therapy (other than thermotherapy, cryotherapy and chemotherapy). The failure rate was 35% (16 of 46) and mortality rate 0%. None of the patients developed CNS manifestations (metastases or trilateral retinoblastoma). One of the patients developed a second primary tumour (osteosarcoma) 4 years following retinoblastoma diagnosis. Altogether 17% (4 of 24) patients received radiation therapy, 28% (13 of 46) of the eyes had to be enucleated, and one patient underwent bilateral enucleation. The age-correlated visual acuity was mean of 73% of expected visual acuity. Conclusion: Group A/B retinoblastomas have a distinct chemotherapy response, while group C/D/E tumours do not respond as well. The success rate was 65%; while patients have a good prognosis for life, approximately one-third of all hereditary cases received radiation therapy or underwent enucleation.

  • 14.
    Berggren, Lennart
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience. Oftalmiatrik.
    Collegium medicum och de kringresande occulisterna2003In: Svensk Medicinhistorisk Tidskrift, Vol. 7, no 1, p. 179-189Article in journal (Other scientific)
  • 15.
    Berggren, Lennart
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience. Oftalmiatrik.
    En berättelse om en läkemedelsskandal2005Other (Other scientific)
  • 16.
    Berggren, Lennart
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience. Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience. Oftalmiatrik.
    Hur ögat uppfattar rörelse - ett favoritämne för både konstnär och neurofysiolog2004In: Läkartidningen, Vol. 101, no 30-31, p. 2430-2432Article in journal (Other scientific)
  • 17.
    Bjärnhall, Gunilla
    Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience.
    Analysis of Angiographies in Human Healthy Eyes and in Open-angle Glaucoma: Retinal Mean Transit Time and Optic Nerve Head Circulation2008Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Purpose of the studies was to develop a more robust technique to determine retinal mean transit time in healthy and in glaucoma eyes and to evaluate the circulation of the optic nerve head in glaucoma patients.

    The retinal mean transit time impulse-response method was evaluated in human healthy eyes and normal values and reproducibility were tested.

    Fluorescein and indocyanine green angiographies were recorded and the pictures were analyzed to obtain retinal mean transit time and to evaluate the proportion of low-fluorescent pixels of the optic nerve head in the glaucoma patients. Visual field defects were correlated to loss of neuroretinal rim area.

    A disturbed circulation was observed in the glaucoma patients, whether primary or secondary to loss of nerve fibre tissue can not be determined from these studies.

    List of papers
    1. Analysis of mean retinal transit time from fluorescein angiography in human eyes: normal values and reproducibilty.
    Open this publication in new window or tab >>Analysis of mean retinal transit time from fluorescein angiography in human eyes: normal values and reproducibilty.
    Show others...
    2002 In: Acta Ophthalmologica Scand, ISSN 1395-3907, Vol. 80, no 6, p. 652-655Article in journal (Refereed) Published
    Identifiers
    urn:nbn:se:uu:diva-97807 (URN)
    Available from: 2008-11-20 Created: 2008-11-20Bibliographically approved
    2. Retinal mean transit time in patients with primary open-angle glaucoma and normal-tension glaucoma
    Open this publication in new window or tab >>Retinal mean transit time in patients with primary open-angle glaucoma and normal-tension glaucoma
    Show others...
    2007 (English)In: Acta Ophthalmologica Scandinavica, ISSN 1395-3907, E-ISSN 1600-0420, Vol. 85, no 1, p. 67-72Article in journal (Refereed) Published
    Abstract [en]

    Purpose: To compare the mean transit time (MTT) of retinal circulation in eyes with primary open-angle glaucoma (POAG) and eyes with normal-tension glaucoma (NTG) and examine the possible relationship between MTT and visual field damage, expressed as mean deviation (MD).

    Methods: Video fluorescein angiography was performed in 40 patients with POAG or NTG. Dye curves for fluorescein passing through the retinal arteries and veins were used to calculate MTT in each patient with a computer-assisted technique based on an impulse-response analysis (MTTir).

    Results: We were able to analyse MTTir in all 40 angiograms. Mean (SD) MTTir was 5.0 (1.5) seconds in eyes with POAG and 4.7 (1.4) seconds in eyes with NTG. The difference was not statistically significant. There was a weak but significant correlation between the MD and MTTir (MTTir = 4.12–0.08*MD; r = −0.49, p = 0.0013).

    Conslusions: The results demonstrate that loss of neuronal tissue in glaucoma is combined with an effect on the retinal circulation and that the effect is similar in eyes with NTG and eyes with POAG.

    Keywords
    normal-tension glaucoma, primary open-angle glaucoma, retinal blood flow, mean transit time, visual field defects, mean deviation
    National Category
    Medical and Health Sciences
    Identifiers
    urn:nbn:se:uu:diva-97808 (URN)10.1111/j.1600-0420.2006.00780.x (DOI)000243634100012 ()17244213 (PubMedID)
    Available from: 2008-11-20 Created: 2008-11-20 Last updated: 2017-12-14Bibliographically approved
    3. The effect of elevated intraocular pressure on the retinal circulation in open-angle glaucoma and ocular hypertension.: A study on retinal mean transit time
    Open this publication in new window or tab >>The effect of elevated intraocular pressure on the retinal circulation in open-angle glaucoma and ocular hypertension.: A study on retinal mean transit time
    Show others...
    Manuscript (Other academic)
    Identifiers
    urn:nbn:se:uu:diva-97809 (URN)
    Available from: 2008-11-20 Created: 2008-11-20 Last updated: 2010-01-13Bibliographically approved
    4. The optic nerve head in open-angle glaucomaand ocular hypertension.: A study with fluorescein and ICG angiography
    Open this publication in new window or tab >>The optic nerve head in open-angle glaucomaand ocular hypertension.: A study with fluorescein and ICG angiography
    Show others...
    Manuscript (Other academic)
    Identifiers
    urn:nbn:se:uu:diva-97810 (URN)
    Available from: 2008-11-20 Created: 2008-11-20 Last updated: 2010-01-13Bibliographically approved
    Download full text (pdf)
    FULLTEXT01
  • 18.
    Blixt, Maria
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Developmental Neuroscience.
    Hallböök, Finn
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Developmental Neuroscience.
    Lineage tracing of horizontal and photoreceptor cells in the embryonic chicken retina2016In: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 57, no 12Article in journal (Refereed)
  • 19.
    Botling Taube, Amelie
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Molecular and epidemiological studies on eyes with pseudoexfoliation syndrome2015Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Pseudoexfoliation (PEX) syndrome is an age-related condition characterized by the production and accumulation of extracellular fibrillary material in the anterior segment of the eye. PEX predisposes for several pathological conditions, such as glaucoma and complications during and after cataract surgery. The pathogenesis of PEX is not yet fully understood. It is multifactorial with genetics and ageing as contributing factors.

    We aimed to study the proteome in aqueous humor (AH) in PEX in order to increase the knowledge about its pathophysiology. Therefore, we developed sampling techniques and evaluated separation methods necessary for analyzing small sample volumes. Other objectives were to study the lens capsule in eyes with PEX regarding small molecules, and to investigate the association between PEX and cataract surgery in a population-based 30-year follow-up study.

    Samples of AH from eyes with PEX and control eyes were collected during cataract surgery. In pooled, and individual samples, various liquid based separation techniques and high resolution mass spectrometry were utilized. For quantitation, various methods for labeling, and label free techniques were applied. Lens capsules were collected from some of the patients, and analysed by imaging mass spectrometry. A cohort of 1,471 elderly individuals underwent a comprehensive ophthalmological examination at baseline. Medical information was obtained by questionnaires, and from medical records. Incident cases of cataract surgery were identified by review of medical records.

    In the initial study, several techniques were explored for protein detection, and a number of proteins were identified as differentially expressed. In the individually labelled samples, changes in the proteome were observed. Eyes with PEX contained higher levels of proteins involved in inflammation, oxidative stress, and coagulation, suggesting that these mechanisms are involved in the pathogenesis in PEX. The levels of β/γ-crystallins were significantly increased in PEX, which is a novel finding. In the lens capsules from individuals with PEX, changes in the lipid composition was observed with time-of-flight secondary ion mass spectrometry. These changes remain to be elucidated. By multivariate analysis, lens opacities were the first, and PEX the second most important predictor for cataract surgery, the later accounting for a 2.38-fold increased risk for cataract surgery.

    List of papers
    1. Protein content in aqueous humor from patients with pseudoexfoliation (PEX) investigated by capillary-LC MALDI-TOF/TOF MS
    Open this publication in new window or tab >>Protein content in aqueous humor from patients with pseudoexfoliation (PEX) investigated by capillary-LC MALDI-TOF/TOF MS
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    2009 (English)In: PROTEOMICS - Clinical Applications, ISSN 1862-8346, E-ISSN 1862-8354, Vol. 3, no 3, p. 299-306Article in journal (Refereed) Published
    Abstract [en]

    Analysis of proteins in human body fluids is challenging since the composition of the sample often is rather complex. Here we present a method for analysis of proteins in aqueous humor from two groups of cataract patients, with and without pseudoexfoliation (PEX). Aqueous humor is an extracellular fluid contained in the anterior chamber of the eye between the cornea and iris. The limited volume of sample requires sophisticated analysis techniques. Our method is based on a total tryptic digestion of the sample followed by capillary LC-MALDI MS and MS/MS analysis of the peptides. The method is rapid, efficient and suitable as a complement or alternative to more commonly used methods based on gel electrophoretic experiments. With this method we found and unambiguously identified 30 nonredundant proteins. Proteins found include general transport proteins such as albumin and apolipoprotein A1 but also specific proteins involved in immune response, such as   complement factors. Cystatin C, clusterin, and crystallins were also found. Although the number of proteins was roughly the same in both groups there was a significant difference in their identities. These findings may give some new insights into the pathophysiology of the PEX syndrome.

    Keywords
    Aqueous humor, Capillary liquid chromatography, Human body fluids, MALDI-TOF MS/MS, Pseudoexfoliation syndrome
    National Category
    Medical and Health Sciences
    Identifiers
    urn:nbn:se:uu:diva-97814 (URN)10.1002/prca.200780077 (DOI)000264794700002 ()
    Available from: 2008-11-20 Created: 2008-11-20 Last updated: 2022-01-28Bibliographically approved
    2. Proteins in aqueous humor from cataract patients with and without pseudoexfoliation syndrome
    Open this publication in new window or tab >>Proteins in aqueous humor from cataract patients with and without pseudoexfoliation syndrome
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    2012 (English)In: European journal of mass spectrometry, ISSN 1469-0667, E-ISSN 1751-6838, Vol. 18, no 6, p. 531-541Article in journal (Refereed) Published
    Abstract [en]

    The aim of this study was to investigate the protein content in aqueous humor in eyes with and without pseudoexfoliations (PEX) and to evaluate the quantitative proteomics method, isobaric tagging for relative and absolute protein quantification (iTRAQ), in combination with two separation methods followed by matrix-assisted Laser desorption/ionization (MALDI) mass spectrometry and tandem mass spectrometry (MS/MS). During cataract surgery, samples of aqueous humor were collected from 20 eyes with PEX and from 18 control eyes. The relative concentrations of proteins in the pooled samples of ten PEX eyes and eight controls were evaluated after trypsin digestion and labeling of the peptides with (iTRAQ) reagent. Two separation methods, Liquid chromatography (LC) and capillary electrophoresis (CE) were used, followed by MALDI mass spectrometry and MS/MS. Furthermore, 1D gel electrophoresis was performed on the remaining ten pooled PEX samples and ten control samples. The gel material was separated by nano-liquid chromatography (nano-LC) followed by Linear-ion-trap quadrupole Fourier transformation ion cyclotron resonance (FT-ICR). Fifty four proteins were identified in the LC runs and 24 with CE. The relative concentrations of beta-crystallines B2 and S were raised and those of angiotensinogen and osteopontin lowered in the PEX sample compared to the control. The trends regarding beta-crystallines B2, angiotensinogen and osteopontin were confirmed by the 1D gel electrophoresis.

    Keywords
    pseudoexfoliations (PEX), isobaric tagging, protein quantification, proteomics, aqueous humour, osteopontin, angiotensinogen
    National Category
    Medical and Health Sciences
    Identifiers
    urn:nbn:se:uu:diva-197670 (URN)10.1255/ejms.1208 (DOI)000315745600007 ()
    Available from: 2013-04-02 Created: 2013-04-02 Last updated: 2022-01-28Bibliographically approved
    3. Proteomic Analysis of the Aqueous Humor in Eyes with Pseudoexfoliation Syndrome
    Open this publication in new window or tab >>Proteomic Analysis of the Aqueous Humor in Eyes with Pseudoexfoliation Syndrome
    (English)Article in journal (Refereed) Submitted
    Keywords
    Aqueous humor; Pseudoexfoliation syndrome; Quantitative proteomics; Dimethyl labelling; Mass spectrometry
    National Category
    Ophthalmology Analytical Chemistry
    Research subject
    Ophtalmology
    Identifiers
    urn:nbn:se:uu:diva-260949 (URN)
    Available from: 2015-08-26 Created: 2015-08-26 Last updated: 2015-10-01
    4. Imaging of Human Lens Capsules with Pseudoexfoliation Syndrome by Time of Flight Secondary Ion Mass Spectrometry (TOF-SIMS)
    Open this publication in new window or tab >>Imaging of Human Lens Capsules with Pseudoexfoliation Syndrome by Time of Flight Secondary Ion Mass Spectrometry (TOF-SIMS)
    (English)Manuscript (preprint) (Other academic)
    Keywords
    PEX, cataract, lens capsule, time of flight secondary mass spectrometry (TOF-SIMS), imaging mass spectrometry (IMS), sphingolipids
    National Category
    Ophthalmology
    Research subject
    Ophtalmology; Chemistry with specialization in Analytical Chemistry
    Identifiers
    urn:nbn:se:uu:diva-260946 (URN)
    Funder
    Swedish Research Council, 621-2011-4423
    Available from: 2015-08-26 Created: 2015-08-26 Last updated: 2015-10-01
    5. Pseudoexfoliation and cataract surgery: a population-based 30-year follow-up study
    Open this publication in new window or tab >>Pseudoexfoliation and cataract surgery: a population-based 30-year follow-up study
    2015 (English)In: Acta Ophthalmologica Scandinavica, ISSN 1395-3907, E-ISSN 1600-0420, Vol. 93, no 8, p. 774-777Article in journal (Refereed) Published
    Abstract [en]

    PURPOSE:

    To study the relationship between pseudoexfoliation (PEX) and cataract surgery in a population in which PEX is a common finding.

    METHODS:

    Survival analyses were performed in a cohort of 760 residents 65-74 years of age, examined in a population survey in the municipality of Tierp, Sweden, during 1984-1986. To expand the cohort, participants in other studies in Tierp were enrolled. Additionally, subjects were recruited by means of glaucoma case records established at the Eye Department in Tierp in 1978-2007. In total, the cohort comprised 1471 individuals, representing more than 15 900 person-years of risk. Information on cataract surgery was obtained from the glaucoma case records and from medical records.

    RESULTS:

    By the end of the study in August 2014, 564 subjects had undergone cataract surgery. Of these cases, 224 were affected by PEX at baseline. In multivariate analyses, PEX was the second most important predictor for cataract surgery after lens opacities, accounting for a 2.38-fold (95% confidence interval 2.01-2.84) increased risk.

    CONCLUSION:

    The results strongly suggest that PEX is a predictor for cataract surgery in the population under study.

    Place, publisher, year, edition, pages
    John Wiley & Sons, 2015
    Keywords
    cataract, cataract surgery, epidemiology, exfoliation syndrome, pseudoexfoliation, risk factor
    National Category
    Ophthalmology
    Research subject
    Ophtalmology
    Identifiers
    urn:nbn:se:uu:diva-260948 (URN)10.1111/aos12789 (DOI)000367654500034 ()
    Available from: 2015-08-26 Created: 2015-08-26 Last updated: 2017-12-04Bibliographically approved
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  • 20.
    Botling Taube, Amelie
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
    Konzer, Anne
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
    Alm, Albert
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Bergquist, Jonas
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
    Proteomic Analysis of the Aqueous Humor in Eyes with Pseudoexfoliation SyndromeArticle in journal (Refereed)
  • 21.
    Botling Taube, Amelie
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience. Karolinska Inst, Dept Clin Neurosci, St Erik Eye Hosp, Stockholm, Sweden.
    Konzer, Anne
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
    Alm, Albert
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Bergquist, Jonas
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
    Proteomic analysis of the aqueous humour in eyes with pseudoexfoliation syndrome2019In: British Journal of Ophthalmology, ISSN 0007-1161, E-ISSN 1468-2079, Vol. 103, no 8, p. 1190-1194Article in journal (Refereed)
    Abstract [en]

    Background/aims Pseudoexfoliation syndrome (PEX) is characterised by the production and accumulation of extracellular fibrillar material in the anterior segment of the eye. The pathogenesis of PEX is multifactorial with genetic factors and ageing as contributing factors. Previously, an increased concentration of beta-crystalline B2 (CRYBB2) was observed in the aqueous humour (AH) in eyes with PEX in a pooled material. Here, the protein content was examined on individual basis. Methods During cataract surgery, AH was sampled from patients with and without PEX, 10 eyes in each group. The proteins were digested and labelled with isotopomeric dimethyl labels, separated with high-pressure liquid chromatography and analysed in an Orbitrap mass analyzer. Results The concentration of complement factor 3, kininogen-1, antithrombin III and vitamin D-binding protein was increased in all eyes with PEX. Retinol-binding protein 3, glutathione peroxidase, calsyntenin-1 and carboxypeptidase E were decreased in eyes with PEX. Beta-crystalline B1 and CRYBB2 and gamma-crystalline D were up to eightfold upregulated in 4 of 10 in eyes with PEX. Conclusion The results indicate that oxidative stress and inflammation are contributing factors in the formation of PEX. Knowledge about the proteome in PEX is relevant for understanding this condition.

    Download full text (pdf)
    fulltext
  • 22.
    Botling Taube, Amelie
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
    Mi, Jia
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
    Alm, Albert
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Bergquist, Jonas
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
    Imaging of Human Lens Capsules with Pseudoexfoliation Syndrome by Time of Flight Secondary Ion Mass Spectrometry (TOF-SIMS)Manuscript (preprint) (Other academic)
  • 23. Breitbart, Eberhardt
    et al.
    Cesarini, Jean-Pierre
    de Gruijl, Frank
    Hietanen, Marja
    Mariutti, G
    Mc Kinley, Alister
    Okuno,
    Roy, C
    Schulmeister, Karl
    Sliney, David
    Söderberg, Per
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Stuck, Bruce
    Swerdlow, Anthony
    Van Deventer, E
    Zeeb, H
    Protecting workers from ultraviolet radiation2007Book (Other academic)
  • 24.
    Brock, Christina
    et al.
    Aalborg Univ, Dept Clin Med, Aalborg, Denmark.;Aalborg Univ Hosp, Dept Gastroenterol & Hepatol, Mech Sense, Aalborg, Denmark.;Steno Diabet Ctr North Denmark, Aalborg, Denmark..
    Wegeberg, Anne-Marie
    Aalborg Univ Hosp, Dept Gastroenterol & Hepatol, Mech Sense, Aalborg, Denmark.;Aalborg Univ Hosp Thisted, Thisted Res Unit, Thisted, Denmark..
    Arendt Nielsen, Thomas
    Aalborg Univ Hosp, Dept Gastroenterol & Hepatol, Mech Sense, Aalborg, Denmark.;Aalborg Univ Hosp, Dept Ophthalmol, Aalborg, Denmark..
    Karout, Bassam
    Milltons Consulting Ltd, Cambridge, England..
    Hellström, Per M.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Gastroenterology/Hepatology.
    Mohr Drewes, Asbjørn
    Aalborg Univ, Dept Clin Med, Aalborg, Denmark.;Aalborg Univ Hosp, Dept Gastroenterol & Hepatol, Mech Sense, Aalborg, Denmark.;Steno Diabet Ctr North Denmark, Aalborg, Denmark..
    Vorum, Henrik
    Aalborg Univ, Dept Clin Med, Aalborg, Denmark.;Aalborg Univ Hosp, Dept Ophthalmol, Aalborg, Denmark.;Aalborg Univ, Dept Clin Med, Hobrovej 18 22, DK-9000 Aalborg, Denmark..
    The Retinal Nerve Fiber Layer Thickness Is Associated with Systemic Neurodegeneration in Long-Term Type 1 Diabetes2023In: Translational Vision Science & Technology, E-ISSN 2164-2591, Vol. 12, no 6, article id 23Article in journal (Refereed)
    Abstract [en]

    Purpose: To determine whether the retinal nerve fiber layer thickness can be used as an indicator for systemic neurodegeneration in diabetes.

    Methods: We used existing data from 38 adults with type 1 diabetes and established polyneuropathy. Retinal nerve fiber layer thickness values of four scanned quadrants (superior, inferior, temporal, and nasal) and the central foveal thickness were extracted directly from optical coherence tomography. Nerve conduction velocities were recorded using standardized neurophysiologic testing of the tibial and peroneal motor nerves and the radial and median sensory nerves, 24-hour electrocardiographic recordings were used to retrieve time- and frequency-derived measures of heart rate variability, and a pain catastrophizing scale was used to assess cognitive distortion.

    Results: When adjusted for hemoglobin A1c, the regional thickness of the retinal nerve fiber layers was (1) positively associated with peripheral nerve conduction velocities of the sensory and motor nerves (all P < 0.036), (2) negatively associated with time and frequency domains of heart rate variability (all P < 0.033), and (3) negatively associated to catastrophic thinking (all P < 0.038).

    Conclusions: Thickness of the retinal nerve fiber layer was a robust indicator for clinically meaningful measures of peripheral and autonomic neuropathy and even for cognitive comorbidity.

    Translational Relevance: The findings indicate that the thickness of the retinal nerve fiber layer should be studied in adolescents and people with prediabetes to determine whether it is useful to predict the presence and severity of systemic neurodegeneration.

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  • 25.
    Brusini, Irene
    et al.
    Medicinsk avbildning, Inst. f. Medicinteknik och hälsosystem, KTH.
    Carrizo, Gabriel
    KTH, Dept. of Biomedical Engineering and Health Systems.
    Bendazzoli, Simone
    KTH, Dept. of Biomedical Engineering and Health Systems.
    Wang, Chunliang
    KTH, Dept. of Biomedical Engineering and Health Systems.
    Yu, Zhaohua
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology. University hospital, Uppsala, Sweden.
    Sandberg Melin, Camilla
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Disciplinary Domain of Medicine and Pharmacy, research centers etc., Centre for Research and Development, Gävleborg.
    Kisonaite, Konstancija
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Raeme, Faisal
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Söderberg, Per
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Fully automatic estimation of the waist of the nerve fiber layer at the optic nerve head angularly resolved2021In: Proceedings Volume 11623, Ophthalmic Technologies XXXI / [ed] Daniel X. Hammer, Karen M. Joos, Daniel V. Palanker, SPIE - International Society for Optical Engineering, 2021, Vol. 11623, p. 1D1-1D8Conference paper (Refereed)
    Abstract [en]

    The present project aims at developing a fully automatic software for estimation of the waist of the nerve fiber layer in the Optic Nerve Head (ONH) angularly resolved in the frontal plane as a tool for morphometric monitoring of glaucoma. The waist of the nerve fiber layer is here defined as Pigment epithelium central limit –Inner limit of the retina – Minimal Distance, (PIMD). 3D representations of the ONH were collected with high resolution OCT in young not glaucomatous eyes and glaucomatous eyes. An improved tool for manual annotation was developed in Python. This tool was found user friendly and to provide sufficiently precise manual annotation. PIMD was automatically estimated with a software consisting of one AI model for detection of the inner limit of the retina and another AI model for localization of the Optic nerve head Pigment epithelium Central limit (OPCL). In the current project, the AI model for OPCL localization was retrained with new data manually annotated with the improved tool for manual annotation both in not glaucomatous eyes and in glaucomatous eyes. Finally, automatic annotations were compared to 3 annotations made by 3 independent annotators in an independent subset of both the not glaucomatous and the glaucomatous eyes. It was found that the fully automatic estimation of PIMD-angle overlapped the 3 manual annotators with small variation among the manual annotators. Considering interobserver variation, the improved tool for manual annotation provided less variation than our original annotation tool in not glaucomatous eyes suggesting that variation in glaucomatous eyes is due to variable pathological anatomy, difficult to annotate without error. The small relative variation in relation to the substantial overall loss of PIMD in the glaucomatous eyes compared to the not glaucomatous eyes suggests that our software for fully automatic estimation of PIMD-angle can now be implemented clinically for monitoring of glaucoma progression.

  • 26.
    Bucht, Curry
    et al.
    Inst. för Klinisk Neurovetenskap, KI.
    Manneberg, Göran
    Biomedicinsk fysik och röntgenfysik, KTH.
    Recording the diffraction pattern reflected from corneal endothelium.2007In: Ophthalmic Technologies XVII / [ed] Manns F, Söderberg PG, Ho Arthur, Bellinghamn, WA, USA: SPIE , 2007, p. 10:1-10:8Conference paper (Refereed)
  • 27. Bucht, Curry
    et al.
    Söderberg, Per G.
    St. Erik's Eye Hospital (Sweden) and Mälarsjukhuset Eskilstuna (Sweden) and Univ. of Miami.
    Manneberg, Göran
    A model for corneal endothelial morphometry by diffraction2006In: Ophthalmic Technologies XVI: Proceedings of SPIE / [ed] Fabrice Manns; Per G. Söderberg; Arthur Ho, San José, CA: SPIE , 2006, Vol. 6138, p. 6138-O-1-6138-O-8Conference paper (Refereed)
  • 28. Bucht, Curry
    et al.
    Söderberg, Per G.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Manneberg, Göran
    Fully automated corneal endothelial morphometry of images captured by clinical specular microscopy2010In: Ophthalmic Technologies XX / [ed] Fabrice Manns, Per G. Söderberg, Arthur Ho, San Francisco, CA: SPIE , 2010, p. 1E-1-1E-8Conference paper (Refereed)
  • 29. Bucht, Curry
    et al.
    Söderberg, Per G.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Manneberg, Göran
    Fully automated corneal endothelial morphometry of images captured by clinical specular microscopy2009In: Ophthalmic Technologies XIX / [ed] Fabrice Manns; Per G. Söderberg; Arthur Ho, San José, CA: SPIE , 2009, Vol. 7163, p. 15:1-15:8Conference paper (Refereed)
  • 30. Bucht, Curry
    et al.
    Söderberg, Per G.
    St. Erik's Eye Hospital (Sweden) and Univ. of Miami.
    Manneberg, Göran
    The impact of horizontal offset of the cornea during corneal specular microscopy2008In: Ophthalmic Technologies XVIII: Proceedings of SPIE / [ed] Fabrice Manns; Per G. Söderberg; Arthur Ho; Bruce E. Stuck; Michael Belkin, San José, CA: SPIE , 2008, Vol. 6426, p. 13-1-13-9Conference paper (Refereed)
  • 31. Bucht, Curry
    et al.
    Söderberg, Per
    St Erik's Eye Hospital (Sweden) and Univ. of Miami.
    Manneberg, Göran
    Recording the dffraction pattern reflected from corneal endothelium2007In:  Ophthalmic Technologies XVII / [ed] Fabrice Manns, Per G. Söderberg, Arthur Ho, Bruce E. Stuck, Michael Belkin, San José, CA: SPIE , 2007, Vol. 6426, p. 10:1-10:8Conference paper (Refereed)
  • 32.
    Bucht, Curry
    et al.
    S:t Eriks Ögonsjukhus, Inst. f. Neurovetenskap, KI.
    Söderberg, Per
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Manneberg, Göran
    Biomedicinsk fysik och röntgenfysik, Inst. för tillämpad fysik.
    Simulation of specular microscopy images of corneal endothelium, a tool for control of measurement errors2011In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 89, no 3, p. e242-e250Article in journal (Refereed)
    Abstract [en]

    Purpose: We aimed at developing simulation software capable of producing images of corneal endothelium close to identical to images captured by clinical specular microscopy with defined morphometrical characteristics. It was further planned to demonstrate the usefulness of the simulator by analysing measurement errors associated with a trained operator using a commercially available semi-automatic algorithm for analysis of simulated images.

    Methods: Software was developed that allows creation of unique images of the corneal endothelium expressing morphology close to identical with that seen in images of corneal specular microscopy. Several hundred unique images of the corneal endothelium were generated with randomization, spanning a physiological range of endothelial cell density. As an example of the usefulness of the simulator for analysis of measurement errors in corneal specular microscopy, a total of 12 of all the images generated were randomly selected such that the endothelial cell density expressed was evenly distributed over the physiological range of endothelial cell density. The images were transferred to a personal computer. The imagenet-640 software was used to analyse endothelial cell size variation, percentage of hexagonal endothelial cells, and endothelial cell density.

    Results: The simulator developed allows randomized generation of corneal specular microscopy images with a preset expected average and variation of cell structure. Calculated morphometric information of each cell is stored in the simulator. The image quality can secondarily be varied with a toolbox of filters to approximate a large spectrum of clinically captured images. As an example of the use of the simulator, measurement errors associated with one trained operator using the imagenet-640 software, and focusing on endothelial cell density, were examined. The functional dependence between morphometric information estimated with the imagenet-640 software algorithm and real morphometric information as provided by the simulator was analysed with regression. It was demonstrated that that the estimations of endothelial cell size variation was associated with a scaling error and that the random error was strongly dependent on the operator.

    Conclusion: The newly developed simulator for randomized generation of morphometrically defined corneal specular microscopy images for the first time makes it possible to estimate a spatial scaling error of an available semi-automatic algorithm and to determine the random measurement error of important morphometric estimates in a defined reference sample of images. It is anticipated that the simulator will be a valuable tool for the generation of a large set of morphometrically well-characterized corneal specular microscopy images that can be used for calibration among research centres, for minimization of random errors and for measurement of quality control. Simulated images will be useful for the development of fully automatic analysis of corneal endothelial cell morphometry.

  • 33.
    Carizzo, Gabriel
    et al.
    KTH, Dept. of Biomedical Engineering and Health Systems.
    Yu, Zhaohua
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Söderberg: Ophthalmic Biophysics.
    Wang, Chunliang
    KTH, Dept. of Biomedical Engineering and Health Systems.
    Sandberg Melin, Camilla
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Söderberg: Ophthalmic Biophysics. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Centre for Research and Development, Gävleborg. Center for Research and Development, Region Gävleborg.
    Kisonaite, Konstancia
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Söderberg, Per
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Söderberg: Ophthalmic Biophysics.
    Fully automatic estimation of the angular distribution of the waist of the nerve fiber layer in the optic nerve head.2020In: SPIE, BIOS, Ophthalmic Technologies XXX / [ed] Manns F, Ho A, Söderberg PG, Bellingham: SPIE , 2020, Vol. 11218Conference paper (Refereed)
  • 34. Castellini, Meryl
    et al.
    Wolf, Louise V.
    Chauhan, Bharesh K.
    Galileo, Deni S.
    Kilimann, Manfred W.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
    Cvekl, A.
    Duncan, Melina K.
    Palm is expressed in both developing and adult mouse lens and retina2005In: BMC Ophthalmology, E-ISSN 1471-2415, Vol. 5, p. 14-Article in journal (Refereed)
    Abstract [en]

    Background

    Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Earlier studies have shown brain preferred Palm expression, although this protein is a major water insoluble protein in chicken lens fiber cells and the Palm gene may be regulated by Pax6.

    Methods

    The expression profile of Palm protein in the embryonic, newborn and adult mouse eye as well as dissociated retinal neurons was determined by confocal immunofluorescence. The relative mRNA levels of Palm, Palmdelphin (PalmD) and paralemmin2 (Palm2) in the lens and retina were determined by real time rt-PCR.

    Results

    In the lens, Palm is already expressed at 9.5 dpc in the lens placode, and this expression is maintained in the lens vesicle throughout the formation of the adult lens. Palm is largely absent from the optic vesicle but is detectable at 10.5 dpc in the optic cup. In the developing retina, Palm expression transiently upregulates during the formation of optic nerve as well as in the formation of both the inner and outer plexiform layers. In short term dissociated chick retinal cultures, Palm protein is easily detectable, but the levels appear to reduce sharply as the cultures age. Palm mRNA was found at much higher levels relative to Palm2 or PalmD in both the retina and lens.

    Conclusion

    Palm is the major paralemmin family member expressed in the retina and lens and its expression in the retina transiently upregulates during active neurite outgrowth. The expression pattern of Palm in the eye is consistent with it being a Pax6 responsive gene. Since Palm is known to be able to drive membrane formation in brain neurons, it is possible that this molecule is crucial for the increase in membrane formation during lens fiber cell differentiation.

  • 35.
    Chiang, Michael F.
    et al.
    NEI, NIH, Bldg 31,Room 6A03,31 Ctr Dr,MSC 2510, Bethesda, MD 20892 USA.
    Quinn, Graham E.
    Univ Penn, Childrens Hosp Philadelphia, Raymond & Ruth Perelman Sch Med, Div Ophthalmol,Scheie Eye Inst, Philadelphia, PA 19104 USA.
    Fielder, Alistair R.
    Univ London, Dept Optometry & Visual Sci, London, England.
    Ostmo, Susan R.
    Oregon Hlth & Sci Univ, Dept Ophthalmol, Casey Eye Inst, Portland, OR 97201 USA.
    Chan, R. V. Paul
    Univ Illinois, Dept Ophthalmol & Visual Sci, Illinois Eye & Infirm, Chicago, IL USA.
    Berrocal, Audina
    Univ Miami, Miller Sch Med, Bascom Palmer Eye Inst, Dept Ophthalmol, Miami, FL 33136 USA.
    Binenbaum, Gil
    Univ Penn, Childrens Hosp Philadelphia, Raymond & Ruth Perelman Sch Med, Div Ophthalmol,Scheie Eye Inst, Philadelphia, PA 19104 USA.
    Blair, Michael
    Retina Consultants Ltd, Des Plaines, IL USA.;Univ Chicago, Dept Ophthalmol, Chicago, IL 60637 USA.
    Campbell, J. Peter
    Oregon Hlth & Sci Univ, Dept Ophthalmol, Casey Eye Inst, Portland, OR 97201 USA.
    Capone, Antonio
    Associated Retinal Consultants PC, Royal Oak, MI USA.;Oakland Univ, William Beaumont Hosp, Sch Med, Dept Ophthalmol, Auburn Hills, MI USA.
    Chen, Yi
    China Japan Friendship Hosp, Dept Ophthalmol, Beijing, Peoples R China.
    Dai, Shuan
    Queensland Childrens Hosp, Dept Ophthalmol, Brisbane, Qld, Australia.
    Ells, Anna
    Calgary Retina Consultants, Calgary, AB, Canada.
    Fleck, Brian W.
    Univ Edinburgh, Dept Ophthalmol, Edinburgh, Midlothian, Scotland.
    Good, William V.
    Smith Kettlewell Eye Res Inst, 2232 Webster St, San Francisco, CA 94115 USA.
    Hartnett, M. Elizabeth
    Univ Utah, Dept Ophthalmol & Visual Sci, John A Moran Eye Ctr, Salt Lake City, UT USA.
    Holmström, Gerd
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Söderberg: Ophthalmic Biophysics.
    Kusaka, Shunji
    Kindai Univ, Dept Ophthalmol, Osakasayama, Japan.
    Kychenthal, Andres
    KYDOFT Fdn, Dept Ophthalmol, Santiago, Chile.
    Lepore, Domenico
    Univ Cattolica Sacro Cuore, Dept Ageing & Neurosci, A Gemelli Fdn IRCSS, Rome, Italy.
    Lorenz, Birgit
    Justus Liebig Univ Giessen, Dept Ophthalmol, Giessen, Germany.;Univ Klinikum Bonn, Dept Ophthalmol, Bonn, Germany.
    Martinez-Castellanos, Maria Ana
    Asociac Evitar Ceguera Mexico, Retina Dept, Mexico City, DF, Mexico.
    Ozdek, Sengul
    Ademola-Popoola, Dupe
    Univ Ilorin, Dept Ophthalmol, Ilorin, Nigeria.
    Reynolds, James D.
    SUNY Buffalo, Dept Ophthalmol, Ross Eye Inst, Buffalo, NY USA.
    Shah, Parag K.
    Aravind Eye Hosp, Dept Pediat Retina & Ocular Oncol, Coimbatore, Tamil Nadu, India.
    Shapiro, Michael
    Retina Consultants Ltd, Des Plaines, IL USA.
    Stahl, Andreas
    Univ Med Greifswald, Dept Ophthalmol, Greifswald, Germany.
    Toth, Cynthia
    Duke Univ, Med Ctr, Dept Ophthalmol, Durham, NC 27710 USA.
    Vinekar, Anand
    Narayana Nethralaya Eye Inst, Dept Pediat Retina, Bangalore, Karnataka, India.
    Visser, Linda
    Univ KwaZulu Natal, Dept Ophthalmol, Durban, South Africa.
    Wallace, David K.
    Indiana Univ Sch Med, Dept Ophthalmol, Indianapolis, IN 46202 USA.
    Wu, Wei-Chi
    Chang Gung Mem Hosp, Dept Ophthalmol, Taoyuan, Taiwan.;Chang Gung Univ, Coll Med, Taoyuan, Taiwan.
    Zhao, Peiquan
    Shanghai Jiao Tong Univ, Xinhua Hosp, Dept Ophthalmol, Sch Med, Shanghai, Peoples R China.
    Zin, Andrea
    Fiocruz MS, Fernandes Figueira Inst, Clin Res Unit, Rio De Janeiro, Brazil.
    International Classification of Retinopathy of Prematurity, Third Edition2021In: Ophthalmology, ISSN 0161-6420, E-ISSN 1549-4713, Vol. 128, no 10, p. 51-68Article in journal (Refereed)
    Abstract [en]

    Purpose: The International Classification of Retinopathy of Prematurity is a consensus statement that creates a standard nomenclature for classification of retinopathy of prematurity (ROP). It was initially published in 1984, expanded in 1987, and revisited in 2005. This article presents a third revision, the International Classification of Retinopathy of Prematurity, Third Edition (ICROP3), which is now required because of challenges such as: (1) concerns about subjectivity in critical elements of disease classification; (2) innovations in ophthalmic imaging; (3) novel pharmacologic therapies (e.g., antievascular endothelial growth factor agents) with unique regression and reactivation features after treatment compared with ablative therapies; and (4) recognition that patterns of ROP in some regions of the world do not fit neatly into the current classification system.

    Design: Review of evidence-based literature, along with expert consensus opinion. Participants: International ROP expert committee assembled in March 2019 representing 17 countries and comprising 14 pediatric ophthalmologists and 20 retinal specialists, as well as 12 women and 22 men.

    Methods: The committee was initially divided into 3 subcommittees-acute phase, regression or reactivation, and imaging-each of which used iterative videoconferences and an online message board to identify key challenges and approaches. Subsequently, the entire committee used iterative videoconferences, 2 in-person multiday meetings, and an online message board to develop consensus on classification.

    Main Outcome Measures: Consensus statement.

    Results: The ICROP3 retains current definitions such as zone (location of disease), stage (appearance of disease at the avascular-vascular junction), and circumferential extent of disease. Major updates in the ICROP3 include refined classification metrics (e.g., posterior zone II, notch, subcategorization of stage 5, and recognition that a continuous spectrum of vascular abnormality exists from normal to plus disease). Updates also include the definition of aggressive ROP to replace aggressive-posterior ROP because of increasing recognition that aggressive disease may occur in larger preterm infants and beyond the posterior retina, particularly in regions of the world with limited resources. ROP regression and reactivation are described in detail, with additional description of long-term sequelae.

    Conclusions: These principles may improve the quality and standardization of ROP care worldwide and may provide a foundation to improve research and clinical care.

  • 36.
    Colafella, Claudio
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
    Figure reuse in Exp Eye Res. (2018); 167: 152–1622019In: Experimental Eye Research, ISSN 0014-4835, E-ISSN 1096-0007, Vol. 181, p. 374-374Article in journal (Other academic)
  • 37.
    Dalin, Frida
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Dermatology and Venereology. Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Stockholm, Sweden..
    Adamus, Grazyna
    Oregon Hlth & Sci Univ, Casey Eye Inst, Ocular Immunol Lab, Portland, OR 97201 USA..
    Yang, Sufang
    Oregon Hlth & Sci Univ, Casey Eye Inst, Ocular Immunol Lab, Portland, OR 97201 USA..
    Landgren, Eva
    Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Stockholm, Sweden..
    Palle, Josefine
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health, Pediatrics.
    Hallgren, Åsa
    Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Stockholm, Sweden..
    Frost, Britt-Marie
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health, Pediatrics.
    Hugosson, Therese
    Lund Univ, Dept Clin Sci, Ophthalmol, Lund, Sweden..
    Landegren, Nils
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Autoimmunity. Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Stockholm, Sweden..
    Eriksson, Daniel
    Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Stockholm, Sweden..
    Andreasson, Sten
    Lund Univ, Dept Clin Sci, Ophthalmol, Lund, Sweden..
    Tabbara, Khalid F.
    Ctr Eye, Riyadh, Saudi Arabia..
    Kämpe, Olle
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Autoimmunity. Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Stockholm, Sweden..
    Alimohammadi, Mohammad
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Dermatology and Venereology. Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Stockholm, Sweden..
    Aryl Hydrocarbon Receptor-Interacting Protein-Like 1 in Cancer-Associated Retinopathy2016In: Ophthalmology, ISSN 0161-6420, E-ISSN 1549-4713, Vol. 123, no 6, p. 1401-1404Article in journal (Other academic)
  • 38.
    Ekesten, Bjorn
    et al.
    Swedish Univ Agr Sci, Dept Clin Sci, POB 7054, SE-75007 Uppsala, Sweden..
    Mäkeläinen, Suvi
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Swedish Univ Agr Sci, Dept Anim Breeding & Genet, Uppsala, Sweden..
    Ellis, Stuart
    Vet Def Soc, Knutsford, England..
    Kjellstrom, Ulrika
    Lund Univ, Dept Clin Sci, Lund, Sweden..
    Bergstrom, Tomas F.
    Swedish Univ Agr Sci, Dept Anim Breeding & Genet, Uppsala, Sweden..
    Abnormal Appearance of the Area Centralis in Labrador Retrievers With an ABCA4 Loss-of-function Mutation2022In: Translational Vision Science & Technology, E-ISSN 2164-2591, Vol. 11, no 2, article id 36Article in journal (Refereed)
    Abstract [en]

    Purpose: To study retinal appearance and morphology in Labrador retrievers (LRs) heterozygous and homozygous for an ABCA4 loss-of-function mutation. Methods: Ophthalmic examination, including ophthalmoscopy and simple testing of vision, was performed in five ABCA4(wt/wt), four ABCA4(wt/InsC), and six ABCA4(InsC/InsC) LRs. Retinas were also examined with confocal scanning laser ophthalmoscopy (cSLO) and optical coherence tomography (OCT). Infrared and fundus autofluorescence (FAF) images were studied, and outer nuclear layer (ONL) and neuroretinal thickness were measured in the central and peripheral area centralis. Results: Clinical signs in young ABCA4(InsC/InsC) LRs were subtle, whereas ophthalmoscopic findings and signs of visual impairment were obvious in old ABCA4InsC/InsC LRs. Retinal appearance and vision testing was unremarkable in heterozygous LRs regardless of age. The cSLO/OCT showed abnormal morphology including ONL thinning, abnormal outer retinal layer segmentation, and focal loss of retinal pigment epithelium in the fovea equivalent in juvenile ABCA4(InsC/InsC) LRs. The abnormal appearance extended into the area centralis and visual streak in middle-aged ABCA4(InsC/InsC) and then spread more peripherally. A mild phenotype was seen on cSLO/OCT and FAF in middle-aged to old ABCA4(wt/InsC) LRs. Conclusions: Abnormal appearance and morphology in the fovea equivalent are present in juvenile ABCA4InsC/InsC. In the older affected LRs, the visual streak and then the peripheral retina also develop an abnormal appearance. Vision deteriorates slowly, but some vision is retained throughout life. Older heterozygotes may show a mild retinal phenotype but no obvious visual impairment. The ABCA4InsC/InsC LR is a potential model for ABCA4-mediated retinopathies/juvenile-onset Stargardt disease in a species with human-sized eyes. Translational Relevance: The ABCA4(InsC) mutation causes juvenile-onset abnormal appearance of the fovea equivalent in affected dogs that slowly spreads in the retina, while only a mild phenotype is seen in older carriers. This is the first non-primate, large animal model for ABCA4-related/STGD1 retinopathies in a species with a fovea equivalent.

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  • 39.
    Ekström, Curt
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Social Medicine.
    Prevalence of open-angle glaucoma in Central Sweden: The Tierp Glaucoma Survey1996In: Acta Ophthalmologica Scandinavica, ISSN 1395-3907, E-ISSN 1600-0420, Vol. 74, no 2, p. 107-112Article in journal (Refereed)
    Abstract [en]

    In 1984‐86, a glaucoma survey was carried out in a municipality in central Sweden, comprising 760 subjects 65–74 years of age. Since 1988, a follow‐up study of those 527 subjects who had normal and reliable visual fields has been in progress. A visual field defect was required for a diagnosis of chronic open‐angle glaucoma. By December 1991, 10 cases of capsular glaucoma and 3 cases of chronic simple glaucoma had been found. The standardized relative risk for chronic open‐angle glaucoma was 3.4 times (95% confidence interval: 1.1 ‐ 11) greater in subjects with a baseline intraocular pressure < 21 mmHg, using the highest pressure in either eye, than in those with an intraocular pressure < 21 mmHg. Pseudoexfoliation increased the standardized relative risk 9.8‐fold (95% confidence interval: 2.5 – 38). There was indication of effect modification between intraocular pressure and pseudoexfoliation. Optic disc haemorrhages were associated with a 4.8‐fold (95% confidence interval: 0.63 – 37) increased unadjusted risk. The study supports the pressure theory of optic nerve damage in glaucoma. Pseudoexfoliation was recognized as an independent risk factor for the development of chronic open‐angle glaucoma.

  • 40.
    Ekström, Curt
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Risk factors for incident open-angle glaucoma: a population-based 20-year follow-up study2012In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 90, no 4, p. 316-321Article in journal (Refereed)
    Abstract [en]

    Purpose: To study the effect of potential risk factors on the development of open-angle glaucoma (OAG) in a population in which pseudoexfoliation (PEX) is a common finding.

    Methods: In 1984-1986, a population-based survey of 760 people aged 65-74 years was conducted in the municipality of Tierp, Sweden. From 1988 to 2006, a follow-up study of the 530 people with normal visual fields has been in progress. To increase the cohort, 273 ophthalmic outpatients were enroled. Reliable visual fields were available for 679 people, representing 6 126 person-years at risk. A time-weighted mean intraocular pressure (IOP) for all visits was calculated.

    Results: Sixty-four subjects developed definite OAG, 29 of whom were exposed to PEX. Risk factors associated with OAG were higher age, a positive family history, increased IOP and PEX. The age-standardized rate ratio (SRR) was 14.8 times (95% confidence interval [CI] 7.92-27.8) greater in subjects with mean IOP >/=20 mmHg than in those with mean IOP /=20 mmHg.

    Conclusion: Increased IOP and PEX were serious risk factors for incident OAG. The effect of PEX was mediated by increased IOP.

  • 41.
    Ekström, Curt
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Botling Taube, Amelie
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Pseudoexfoliation and cataract surgery: a population-based 30-year follow-up study2015In: Acta Ophthalmologica Scandinavica, ISSN 1395-3907, E-ISSN 1600-0420, Vol. 93, no 8, p. 774-777Article in journal (Refereed)
    Abstract [en]

    PURPOSE:

    To study the relationship between pseudoexfoliation (PEX) and cataract surgery in a population in which PEX is a common finding.

    METHODS:

    Survival analyses were performed in a cohort of 760 residents 65-74 years of age, examined in a population survey in the municipality of Tierp, Sweden, during 1984-1986. To expand the cohort, participants in other studies in Tierp were enrolled. Additionally, subjects were recruited by means of glaucoma case records established at the Eye Department in Tierp in 1978-2007. In total, the cohort comprised 1471 individuals, representing more than 15 900 person-years of risk. Information on cataract surgery was obtained from the glaucoma case records and from medical records.

    RESULTS:

    By the end of the study in August 2014, 564 subjects had undergone cataract surgery. Of these cases, 224 were affected by PEX at baseline. In multivariate analyses, PEX was the second most important predictor for cataract surgery after lens opacities, accounting for a 2.38-fold (95% confidence interval 2.01-2.84) increased risk.

    CONCLUSION:

    The results strongly suggest that PEX is a predictor for cataract surgery in the population under study.

  • 42.
    Ekström, Curt
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Ophthalmic Biophsics.
    Hårleman, Karl
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Ophthalmic Biophsics.
    Risk factors for incident open-angle glaucoma in clinical practice in Sweden: A population-based case-control study2023In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 101, no 5, p. 530-535Article in journal (Refereed)
    Abstract [en]

    Purpose To study predictors of open-angle glaucoma (OAG) in newly diagnosed patients detected in clinical practice in a defined population and to estimate the frequency of normal-tension glaucoma (NTG).

    Methods Predictors of OAG were analysed in a case-control study, nested in a cohort of 481 people, 55-84 years of age, in two rural districts in Sweden, examined at the Eye Department in Tierp from 1988 to 2003. To make the sample complete, 63 residents first examined at the Uppsala University Hospital (UUH), 60 of whom were identified with the help of registers, were invited to attend the study. In this way, the cohort included 544 individuals. Automated perimetry was used to find cases of OAG.

    Results In people first examined in Tierp, incident OAG was found in 99 subjects. Of these cases, six were diagnosed with NTG. Increased intraocular pressure (IOP), higher age, male sex, a positive family history, pseudoexfoliation (PEX), and haemorrhages of the optic disc were associated with OAG. In a logistic regression model including PEX, every increase in IOP by 5 mmHg increased the risk for OAG four-fold (odds ratio 4.04; 95% confidence interval 2.91-5.62). The effect of PEX was essentially mediated by increased pressure. The inclusion of the 63 subjects first examined at the UUH had no impact on the results.

    Conclusion In this study on patients aged 55-84-years old in clinical practice in Sweden, increased IOP was strongly associated with incident OAG, while NTG was a rare finding.

  • 43.
    Ekström, Curt
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Kilander, Lena
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Geriatrics. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Open-angle glaucoma and Alzheimer´s disease: a population-based 30 year follow-up study2017In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 95, no 2, p. E157-E158Article in journal (Other academic)
  • 44.
    Ekström, Curt
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Kilander, Lena
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Geriatrics.
    Pseudoexfoliation and Alzheimer´s disease: a population-based 30-year follow-up study2014In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 92, no 4, p. 355-358Article in journal (Refereed)
  • 45.
    Ekström, Curt
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Söderberg: Ophthalmic Biophysics.
    Ustrup, Jasper Grunditz
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Söderberg: Ophthalmic Biophysics.
    Association between Age-related Cataract and Mortality in Sweden: A Long-term Population-based Follow-up Study2021In: Ophthalmic Epidemiology, ISSN 0928-6586, E-ISSN 1744-5086, Vol. 28, no 4, p. 301-305Article in journal (Refereed)
    Abstract [en]

    Purpose To assess the relationship of age-related cataract with all-cause mortality in a Swedish population. Methods Cox regression analyses were performed in a cohort of 746 residents 65-74 years of age, examined in a population survey in the rural district of Tierp, Sweden, 1984-86. To expand the sample size, 1,071 people were recruited by means of glaucoma case records established at the Eye Department in Tierp from 1978 to 2007. In this way, the cohort comprised 1,817 subjects, representing nearly 27,000 person-years at risk. The presence of cataract was determined based on retroillumination with lens opacities evident on slit-lamp examination. Information on deaths was obtained from the local population register. Results By the conclusion of the study in April 2020, 1,633 deaths had been reported. Of these cases, 694 were affected by lens opacities or had history of cataract surgery at baseline. In multivariate analysis, including cataract, age, sex, smoking habits, cancer, diabetes, hypertension and ischemic heart disease, no association was found between cataract and mortality (hazard ratio 0.99; 95% confidence interval 0.90-1.10). Adjustment for participation in the population survey had no effect on the estimate. Conclusion In this long-term follow-up study of subjects 65-74-years-old in Sweden, cataract was not associated with all-cause mortality.

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  • 46.
    Ekström, Curt
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Wilger, Sophia
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Wanhainen, Anders
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Vascular Surgery.
    Pseudoexfoliation and aortic aneurysm: a long-term follow-up study2019In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 97, no 1, p. 80-83Article in journal (Refereed)
    Abstract [en]

    PURPOSE: To study the relationship between pseudoexfoliation (PEX) and aortic aneurysm in a population with a high prevalence of PEX.

    METHODS: Survival analyses were performed in a cohort of 735 residents aged 65-74 years, examined in a population survey in the municipality of Tierp, Sweden, 1984-1986. To expand the sample size, 1040 people were recruited by means of glaucoma case records established at the Eye Department in Tierp in 1978-2007. In this way, the cohort comprised 1775 subjects, representing more than 25 400 person-years at risk. Medical records and autopsy reports were reviewed to identify subjects diagnosed with aneurysm. Those with a follow-up time shorter than 1 year were excluded.

    RESULTS: By the end of the study in September 2017, 60 new cases of aortic aneurysm had been found. Of these cases, 23% (14 subjects) were affected by PEX at baseline, compared with 28% among subjects without aneurysm. No association between PEX and aortic aneurysm was found (hazard ratio 0.97; 95% confidence interval 0.53-1.77).

    CONCLUSION: In this population-based study, we were unable to verify a relationship between PEX and the development of aortic aneurysm.

  • 47.
    Eriksson, Urban
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Aspects of Optical Coherence Tomography (OCT) in Healthy Eyes and Eyes with Retinal Diseases2010Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Optical coherence tomography (OCT) is a technique in which cross-sectional images from intraocular tissue can be obtained. The quantitative and qualitative examinations are used for evaluating retinal diseases. Conventional OCT (Stratus) is mainly used, but the new Spectral domain (Cirrus) OCT, which has improved technology, may provide more reliable measurements.

    The aim of the study was to collect normal values of macular thickness in children and adults and to evaluate the effect of age and/or gender, to compare measurement variability in healthy eyes and eyes with age-related macular degeneration (AMD), to compare Stratus and Cirrus OCT and to study the effect of cataract surgery on macula.

    Sixty-seven healthy adults and 56 children, 30 patients with AMD, 34 patients with diabetes and cataract and 35 healthy controls were included. The quantitative maps in Stratus and Cirrus were used and manual correction of foveal location was evaluated. Qualitative OCT was compared to fluorescein angiography (FA) after cataract surgery.

    The mean values of macular thickness in Stratus OCT were 207µm in adults and 204 µm in children. The measurement variability was low. Macular thickness decreased with age in adults, but not in children. No correlation with gender was found. In eyes with wet AMD, there were small differences in measurement variability comparing Stratus and Cirrus OCT. After manual correction in Cirrus OCT, the coefficients of repeatability were improved to values close to the repeatability in normal eyes. Two thirds of the diabetic and half of the control eyes showed leakage on FA after cataract surgery. Qualitative OCT corresponded poorly to FA in diabetic eyes. A thicker macula, assessed with OCT, was often observed without any obvious effect on visual acuity.  OCT was as good as FA in revealing clinically relevant changes in macula after surgery, and was the technique recommended for follow-up.

    List of papers
    1. Repeatability in and interchangeability between the macular and the fast macular thickness map protocols: a study on normal eyes with Stratus optical coherence tomography
    Open this publication in new window or tab >>Repeatability in and interchangeability between the macular and the fast macular thickness map protocols: a study on normal eyes with Stratus optical coherence tomography
    2009 (English)In: Acta ophthalmologica, ISSN 1755-3768, Vol. 87, no 7, p. 725-730Article in journal (Refereed) Published
    Abstract [en]

    Purpose: To collect a normal material and to compare the macular and the fast macular thickness map protocols regarding normal values and repeatability. Methods: Sixty-seven individuals underwent three repeated scans with the macular thickness protocol; 45 of them also had three scans with the fast thickness protocol in Stratus optical coherence tomography (OCT). The maps were divided into nine ETDRS fields, where thickness values were presented. The repeatability was calculated as intraclass correlation coefficient (ICC), coefficient of variance (CV) and coefficient of repeatability (CR). For comparison between the two protocols, limits of agreement were determined according to Bland-Altman. Results: Normal values for the two protocols were very close. Repeatability was high. ICC for all areas was 0.92-0.98. CV was less than 1% and CR was 6-8 mum for both protocols, with the exception of the fovea in the fast protocol (where CV was 1.44% and CR 12.4 mum). Limits of agreement between the two protocols were less than 10 mum as a rule. Conclusion: Normal values for the protocols are equal and they both have excellent repeatability. The fast macular map is a good alternative with the possible exception of the fovea, where variation is twice that of the macular thickness map.

    Keywords
    macular thickness maps, normal values, optical coherence tomography, repeatability
    National Category
    Medical and Health Sciences
    Identifiers
    urn:nbn:se:uu:diva-104911 (URN)10.1111/j.1755-3768.2008.01345.x (DOI)000270999300006 ()18937816 (PubMedID)
    Available from: 2009-05-30 Created: 2009-05-30 Last updated: 2022-01-28Bibliographically approved
    2. A population-based study of macular thickness in full-term children assessed with Stratus OCT: normative data and repeatability.
    Open this publication in new window or tab >>A population-based study of macular thickness in full-term children assessed with Stratus OCT: normative data and repeatability.
    2009 (English)In: Acta ophthalmologica, ISSN 1755-3768, Vol. 87, no 7, p. 741-745Article in journal (Refereed) Published
    Abstract [en]

    Purpose: We aimed to determine normal macular thickness values, assessed with optical coherence tomography (OCT), in a population of full-term children of normal birthweight. Methods: A total of 56 children, aged 5-16 years, randomly chosen from the population register, were examined with Stratus OCT. Only children with visual acuity < 0.2 logMAR, spherical equivalent of - 3 to + 3 D and astigmatism < 2 D were included. The fast macular map protocol was used and three examinations were performed in each eye. One eye was then randomized for further analyses. Mean values for the nine ETDRS areas, foveal minimum thickness and macular volume were calculated for 55 eyes. Coefficients of variance and intraclass correlations were calculated for each area. Results: All children co-operated well and no child was excluded for lack of concentration. Mean +/- standard deviation central macular thickness was 204 +/- 19 mum. Mean total macular volume was 7.11 +/- 0.35 mm(3). No correlations were found between age, gender and macular thickness. Coefficients of variance were < 2% and intraclass correlations were > 0.9 in all areas, except the foveal minimum. Conclusions: Normal values for macular thickness in healthy full-term children were reported. As the Stratus OCT provides normal values only for adults, these data are a better alternative for comparison with children with retinal abnormalities. We concluded that OCT is suitable for examining the retina in children aged 5-16 years and has the same high level of repeatability as in adults.

    Keywords
    children, full-term, macular thickness, optical coherence tomography (OCT), repeatability
    National Category
    Medical and Health Sciences
    Identifiers
    urn:nbn:se:uu:diva-104400 (URN)10.1111/j.1755-3768.2008.01357.x (DOI)000270999300009 ()18811639 (PubMedID)
    Available from: 2009-05-30 Created: 2009-05-28 Last updated: 2022-01-28Bibliographically approved
    3. Macular thickness decreases with age in normal eyes: A study on the macular thickness map protocol in the Stratus OCT
    Open this publication in new window or tab >>Macular thickness decreases with age in normal eyes: A study on the macular thickness map protocol in the Stratus OCT
    2008 (English)In: British Journal of Ophthalmology, ISSN 0007-1161, E-ISSN 1468-2079, Vol. 93, no 11, p. 1148-1452Article in journal (Refereed) Published
    Abstract [en]

    BACKGROUND/AIM: Retinal and retinal nerve fibre layer (RNFL) thinning with age have been described in histological studies. In vivo techniques like optical coherence tomography (OCT), has shown thinning of optic nerve RNFL and the retina in specific areas. One would expect thinning of the total macula, but so far, a correlation with the quantitative OCT macular map tool and age has not been found. METHODS: Sixty-seven healthy individuals underwent three repeated scans in both eyes with the macular thickness map protocol in the Stratus OCT. That protocol divides the macula area in nine ETDRS fields.The RNFL was measured in one specific location close to the optic disc. Correlations between retinal, RNFL thickness, macular volume and age were determined. RESULTS: We found a statistically significant negative relationship between retinal thickness and age for all ETDRS areas, total macular volume and RNFL thickness. Retinal thickness decreased with 0,26-0,46 microm, the macula volume 0,01 mm(3) and RNFL with 0,09 microm per year. CONCLUSION: Retinal thickness within the area covered by the macular map significantly decreases with age. In the area examined in the papillomacular bundle, 20% of the retinal thinning is due to the RNFL and 80% due to thinning of other layers of the retina.

    National Category
    Medical and Health Sciences
    Identifiers
    urn:nbn:se:uu:diva-104910 (URN)10.1136/bjo.2007.131094 (DOI)000271091900009 ()19019921 (PubMedID)
    Available from: 2009-05-30 Created: 2009-05-30 Last updated: 2022-01-28Bibliographically approved
    4. Is quantitative spectral-domain superior to time-domain optical coherence tomography (OCT) in eyes with age-related macular degeneration?
    Open this publication in new window or tab >>Is quantitative spectral-domain superior to time-domain optical coherence tomography (OCT) in eyes with age-related macular degeneration?
    2012 (English)In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 90, no 7, p. 620-627Article in journal (Refereed) Published
    Abstract [en]

    Purpose: The aim of this study was to determine the variability of macular map measurements, for two generations of optical coherence tomography (OCT) instruments, in eyes with wet age related macular degeneration (AMD) and low visual acuity. Methods: Patients were examined with Stratus OCT and Cirrus HD-OCT. The macular thickness was assessed with the 'macular thickness map scan' and 'fast protocol' in Stratus and with the 512 × 128 and 200 × 200 cube protocols in Cirrus OCT. Two measurements were taken one directly after the other, at the first visit to analyse repeatability. Approximately 1 week later, a third measurement was taken to analyse reproducibility. In Cirrus OCT, a manual correction of foveal location was also performed. Repeatability and reproducibility were calculated as a coefficient of variance (CoV) and a coefficient of repeatability/reproducibility. Results: Repeatability for central macular thickness (expressed as CoV) was about three per cent for all protocols, and the coefficient of repeatability between 34 and 54 μm. Reproducibility (also expressed as CoV) was between four to seven per cent and coefficient of repeatability between 64 and 89 μm. After manual adjustment of foveal location in Cirrus OCT, the coefficient of repeatability improved to 12-18 μm, and the coefficient of reproducibility to 44-47 μm. Conclusions: In eyes affected by wet AMD, there were small differences in repeatability and reproducibility when comparing quantitative maps in Stratus and Cirrus OCT. However, when the software for manual correction of foveal position in Cirrus OCT was used, the variability decreased markedly, and the repeatability was close to what had been reported in normal eyes, demonstrating a significant, potential advantage of spectral-domain over time-domain OCT.

    Keywords
    age-related macular degeneration, quantitative optical coherence tomography, repeatability, reproducibility
    National Category
    Ophthalmology
    Research subject
    Ophtalmology
    Identifiers
    urn:nbn:se:uu:diva-184945 (URN)10.1111/j.1755-3768.2011.02112.x (DOI)000310548500017 ()
    Available from: 2012-11-19 Created: 2012-11-15 Last updated: 2017-12-07Bibliographically approved
    5. Macular edema and visual outcome following cataract surgery in patients with diabetic retinopathy and controls
    Open this publication in new window or tab >>Macular edema and visual outcome following cataract surgery in patients with diabetic retinopathy and controls
    Show others...
    2011 (English)In: Graefe's Archives for Clinical and Experimental Ophthalmology, ISSN 0721-832X, E-ISSN 1435-702X, Vol. 249, no 3, p. 349-359Article in journal (Refereed) Published
    Abstract [en]

    Cystoid macular edema (CME) is a well-known complication after cataract surgery, and diabetic retinopathy is reported to be an important risk factor for impaired visual recovery. In this prospective study, we compared visual outcome 6 months after surgery in eyes with moderate retinopathy and no previous ME with a control group, and observed the incidence of ME seen on fluorescein angiography (FA) and optical coherence tomography (OCT).

    Thirty-four patients with type-2 diabetes and 35 controls were enrolled. Best-corrected visual acuity (VA) letters ETDRS was measured pre-op, at day 7, week 6 and month 6. FA performed pre-op and at week 6 was divided into three leakage patterns. OCT performed pre-op, at week 6 and month 6 was qualitatively divided into three types. Macular thickness was measured in three circular fields (central subfield, inner and outer circle) from the macular maps.

    There was no statistically significant difference in VA before surgery, at day 7 or at 6 months, but at 6 weeks there was a significant difference with lower VA in the diabetic group. Six percent of control and 12% of diabetic eyes developed a clinical CME defined as a loss of > 5 letters between day 7 and week 6. Incidence of FA leakage was 23% in control and 76% in diabetic eyes. At 6 weeks, 20% of control and 44% of the diabetic eyes had qualitative changes on OCT. A statistically significant increase in thickness was observed for all three macular areas in both groups, part of it remaining at 6 months. There were, however, no differences in central macular thickness between the groups at any visit. Retinal thickening had poor correlation with VA.

    The final visual outcome in eyes with mild to moderate retinopathy, without previous ME, is as good as in normal eyes, but an increased frequency of macular changes may protract recovery of full vision. Changes on OCT or FA are often seen without any obvious effect on VA. OCT is as good as FA at detecting a clinical CME, and is the technique recommended for follow-up before FA is considered.

    Keywords
    Visual outcome, Phacoemulsification, Cystoid macular edema, Optical coherence tomography, Fluorescein angiography, Sub-clinical macular thickening
    National Category
    Medical and Health Sciences
    Research subject
    Ophtalmology
    Identifiers
    urn:nbn:se:uu:diva-128901 (URN)10.1007/s00417-010-1484-9 (DOI)000288454200003 ()20827486 (PubMedID)
    Available from: 2010-08-03 Created: 2010-08-02 Last updated: 2022-01-28Bibliographically approved
    Download full text (pdf)
    FULLTEXT01
  • 48.
    Eriksson, Urban
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Alm, Albert
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Larsson, Eva
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Is quantitative spectral-domain superior to time-domain optical coherence tomography (OCT) in eyes with age-related macular degeneration?2012In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 90, no 7, p. 620-627Article in journal (Refereed)
    Abstract [en]

    Purpose: The aim of this study was to determine the variability of macular map measurements, for two generations of optical coherence tomography (OCT) instruments, in eyes with wet age related macular degeneration (AMD) and low visual acuity. Methods: Patients were examined with Stratus OCT and Cirrus HD-OCT. The macular thickness was assessed with the 'macular thickness map scan' and 'fast protocol' in Stratus and with the 512 × 128 and 200 × 200 cube protocols in Cirrus OCT. Two measurements were taken one directly after the other, at the first visit to analyse repeatability. Approximately 1 week later, a third measurement was taken to analyse reproducibility. In Cirrus OCT, a manual correction of foveal location was also performed. Repeatability and reproducibility were calculated as a coefficient of variance (CoV) and a coefficient of repeatability/reproducibility. Results: Repeatability for central macular thickness (expressed as CoV) was about three per cent for all protocols, and the coefficient of repeatability between 34 and 54 μm. Reproducibility (also expressed as CoV) was between four to seven per cent and coefficient of repeatability between 64 and 89 μm. After manual adjustment of foveal location in Cirrus OCT, the coefficient of repeatability improved to 12-18 μm, and the coefficient of reproducibility to 44-47 μm. Conclusions: In eyes affected by wet AMD, there were small differences in repeatability and reproducibility when comparing quantitative maps in Stratus and Cirrus OCT. However, when the software for manual correction of foveal position in Cirrus OCT was used, the variability decreased markedly, and the repeatability was close to what had been reported in normal eyes, demonstrating a significant, potential advantage of spectral-domain over time-domain OCT.

  • 49.
    Farhoudi, Daniel B.
    et al.
    Karolinska Inst, St Erik Eye Hosp, Div Ophthalmol & Vis, Dept Clin Neurosci, Stockholm, Sweden.
    Behndig, Anders
    Umea Univ Hosp, Dept Clin Sci Ophthalmol, Umea, Sweden.
    Mollazadegan, Kaziwe
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Ophthalmology.
    Montan, Per
    Karolinska Inst, St Erik Eye Hosp, Div Ophthalmol & Vis, Dept Clin Neurosci, Stockholm, Sweden.
    Lundstrom, Mats
    Lund Univ, Dept Clin Sci, Ophthalmol, Fac Med, Lund, Sweden.
    Kugelberg, Maria
    Karolinska Inst, St Erik Eye Hosp, Div Ophthalmol & Vis, Dept Clin Neurosci, Stockholm, Sweden.
    Spectacle use after routine cataract surgery and vision-related activity limitation2018In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 96, no 6, p. 582-585Article in journal (Refereed)
    Abstract [en]

    Purpose To explore the relationship between acquisition of new spectacles after routine cataract surgery and vision-related activity limitation (VRAL) postoperatively. MethodsResultsThis cohort study with intervention (survey) included 1329 patients in Sweden who had undergone a second-eye cataract surgery during March 2013. Data from the Swedish National Cataract Register were used, including evaluations of VRAL through the Catquest-9SF questionnaire before and 3months after cataract surgery. Five months after the second-eye surgery, patients completed another five-item questionnaire about spectacle use preoperatively and postoperatively including an item on surgeons' advice about the need for spectacles. These responses were linked to the Rasch-analysed Catquest-9SF data to identify correlations with VRAL. A total of 1239 patients finally participated in the study after excluding those who did not fulfil the inclusion criteria. Patients who were advised about the need for spectacles postoperatively (n=387) had a greater (p=0.039) improvement in the postoperative VRAL compared to patients who were not advised (n=691). Patients who obtained new spectacles postoperatively (n=512) also had greater improvement (p=0.032) compared to those who did not (n=724). ConclusionThe average improvements in the VRAL after surgery were significantly higher for patients who obtained new distance spectacles postoperatively and for patients who were informed about the need for spectacles by their practitioners.

  • 50.
    Fielder, Alistair R.
    et al.
    City Univ London, Dept Optometry Visual Sci, London, England..
    Quinn, Graham E.
    Univ Penn, Childrens Hosp Philadelphia, Scbeie Eye Inst, Raymond & Ruth Perelman Sch Med,Div Ophthalmol, Philadelphia, PA USA..
    Chan, R. V. Paul
    Univ Illinois, Illinois Eye & Ear Infirm, Dept Ophthalmol & Visual Sci, Chicago, IL USA..
    Holmström, Gerd
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Ophthalmic Biophsics.
    Chiang, Michael F.
    NEI, NIH, Bethesda, MD USA..
    Retinopathy of prematurity classification updates: possible implications for treatment2022In: Journal of AAPOS, ISSN 1091-8531, E-ISSN 1528-3933, Vol. 26, no 3, p. 109-112Article in journal (Refereed)
123456 1 - 50 of 266
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