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  • 1.
    Pavliuk, Mariia
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Accumulative Charge Separation in Photocatalysis: From Molecules to Nanoparticles2019Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Photochemical energy conversion into solar fuel involves steps of light absorption, charge separation and catalysis. Nature has taught us that the effective accumulation of redox equivalents and charge separation are the key steps in sunlight conversion. The focus of this thesis is to unveil photophysical and photochemical processes that lead to accumulative charge separation. The optimization of electron transfer process will be held by minimization of losses via recombination, and extension of the lifetime of the charge separated state by usage of the electron relay.

    The goal is to couple light induced electron transfer process with the multi-electron catalytic process of hydrogen evolution. In this regard, light harvesters (molecules, metal nanostructures) that generate at least two electrons per absorbed photon will be studied. Additionally, semiconductors that generate long-lived charge separated states are utilized to accumulate several redox equivalents necessary for hydrogen evolution.

    The hybrid systems produced by the combination of the advantageous properties of molecules, semiconductors, and metal nanoparticles are under the scope of investigation. Metal nanoparticles are advantageous because of their high absorption cross-section. The molecular linkers provide control and flexibility in tuning the connection between the light absorber and the electron relay. Semiconductor nanoparticles offer the desired charge separation properties via prolonging the lifetime sufficiently to perform photocatalysis.

    The detailed understanding, investigation and development of the hybrid systems is at the heart of the progress of photochemical solar fuel production.

    Delarbeten
    1. Ultra long-lived electron-hole separation within water-soluble colloidal ZnO nanocrystals: Prospective Applications For Solar Energy Production
    Öppna denna publikation i ny flik eller fönster >>Ultra long-lived electron-hole separation within water-soluble colloidal ZnO nanocrystals: Prospective Applications For Solar Energy Production
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    2016 (Engelska)Ingår i: Nano Energy, ISSN 2211-2855, Vol. 30, s. 187-192Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Zinc oxide was one of the first semiconductors used in dye-sensitized solar cells but its instability in aqueous media precludes its use for large-scale applications. Herein, we report on a novel ZnO nanocrystal material derived by an organometallic approach that is simultaneously stable and soluble in water due to its carboxylate oligoethylene glycol shell strongly anchored to the inorganic core by the head groups. The resulting unique inorganic core-organic shell interface also stabilizes the photo-generated hole, leading to a dramatic slowing down of charge recombination, which otherwise is a major hurdle in using nanostructured ZnO.

    Nyckelord
    Water-soluble colloidal ZnO nanocrystals, Ultra long-lived electron-hole separation, Advanced laser based spectroscopy, Solar energy production prospectus
    Nationell ämneskategori
    Nanoteknik Fysikalisk kemi
    Identifikatorer
    urn:nbn:se:uu:diva-314050 (URN)10.1016/j.nanoen.2016.09.045 (DOI)000390636100023 ()
    Forskningsfinansiär
    Knut och Alice Wallenbergs StiftelseEU, Europeiska forskningsrådet, 687008Vetenskapsrådet, 2015-03764
    Tillgänglig från: 2017-02-06 Skapad: 2017-01-26 Senast uppdaterad: 2018-12-17Bibliografiskt granskad
    2. Hydrogen evolution with nanoengineered ZnO interfaces decorated using a beetroot extract and a hydrogenase mimic
    Öppna denna publikation i ny flik eller fönster >>Hydrogen evolution with nanoengineered ZnO interfaces decorated using a beetroot extract and a hydrogenase mimic
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    2017 (Engelska)Ingår i: Sustainable Energy & Fuels, ISSN 2398-4902, Vol. 1, s. 69-73Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Herein, we report a nano-hybrid photo-system based on abundant elements for H2 production with visible light. The photo-system's proficiency relates to the novel ZnO nanocrystals employed. The ZnO carboxylate oligoethylene glycol shell enhances charge separation and accumulates reactive electrons for the photocatalytic process. 

    Ort, förlag, år, upplaga, sidor
    Royal Society of Chemistry, 2017
    Nyckelord
    H2 evolution
    Nationell ämneskategori
    Fysikalisk kemi Teknik och teknologier
    Forskningsämne
    Kemi med inriktning mot fysikalisk kemi
    Identifikatorer
    urn:nbn:se:uu:diva-335979 (URN)10.1039/c6se00066e (DOI)000422769100006 ()
    Tillgänglig från: 2017-12-11 Skapad: 2017-12-11 Senast uppdaterad: 2018-12-17Bibliografiskt granskad
    3. Magnetic Manipulation of Spontaneous Emission from Inorganic CsPbBr3 Perovskites Nanocrystals
    Öppna denna publikation i ny flik eller fönster >>Magnetic Manipulation of Spontaneous Emission from Inorganic CsPbBr3 Perovskites Nanocrystals
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    2016 (Engelska)Ingår i: ADVANCED OPTICAL MATERIALS, ISSN 2195-1071, Vol. 4, nr 12, s. 2004-2008Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Metal halide perovskites have shown great potential for both light-absorbing and light-emitting devices. It is demonstrated that the presence of a low-magnetic field decreases dramatically the photoluminescence of CsPbBr3. This is found to be due to a decrease in charge separated state lifetime. The effect is fully reversible, and can be exploited for simple and remote modulation of the output of light-emitting devices.

    Nationell ämneskategori
    Fysikalisk kemi Materialkemi
    Identifikatorer
    urn:nbn:se:uu:diva-315916 (URN)10.1002/adom.201600611 (DOI)000392405100014 ()
    Forskningsfinansiär
    Knut och Alice Wallenbergs StiftelseVetenskapsrådet, 2015-03764EU, FP7, Sjunde ramprogrammet, 306733
    Tillgänglig från: 2017-02-23 Skapad: 2017-02-23 Senast uppdaterad: 2018-12-17Bibliografiskt granskad
    4. Hydrogen evolution with CsPbBr3 perovskite nanocrystals under visible light in solution
    Öppna denna publikation i ny flik eller fönster >>Hydrogen evolution with CsPbBr3 perovskite nanocrystals under visible light in solution
    2018 (Engelska)Ingår i: MATERIALS TODAY COMMUNICATIONS, ISSN 2352-4928, Vol. 16, s. 90-96Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Direct proton photo-reduction to molecular hydrogen with a lead-halide perovskite photosystem is presented. The concept uses CsPbBr3 nanocrystals and Ru@TiO2 nanoparticles as light harvesters and catalyst, respectively. The photo-system attains charge transfer from donor to acceptor via collision events, established via static and ultrafast spectroscopy. The photo-system exhibits a photon-to-hydrogen efficiency of ca. 0.4%, a respectable efficiency for a system relying on effective collisions for the transference of electrons.

    Nyckelord
    Inorganic perovskites nanocrystals, H-2 evolution, Ultrafast spectroscopy, Charge transfer via collision
    Nationell ämneskategori
    Materialkemi Fysikalisk kemi Den kondenserade materiens fysik
    Identifikatorer
    urn:nbn:se:uu:diva-364171 (URN)10.1016/j.mtcomm.2018.05.001 (DOI)000443035000012 ()
    Forskningsfinansiär
    VetenskapsrådetStiftelsen Olle Engkvist Byggmästare
    Tillgänglig från: 2018-11-06 Skapad: 2018-11-06 Senast uppdaterad: 2018-12-17Bibliografiskt granskad
    5. Nano-hybrid plasmonic photocatalyst for hydrogen production at 20% efficiency
    Öppna denna publikation i ny flik eller fönster >>Nano-hybrid plasmonic photocatalyst for hydrogen production at 20% efficiency
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    2017 (Engelska)Ingår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, artikel-id 8670Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    The efficient conversion of light energy into chemical energy is key for sustainable human development. Several photocatalytic systems based on photovoltaic electrolysis have been used to produce hydrogen via water reduction. However, in such devices, light harvesting and proton reduction are carried separately, showing quantum efficiency of about 10–12%. Here, we report a nano-hybrid photocatalytic assembly that enables concomitant reductive hydrogen production and pollutant oxidation with solar-to-fuel efficiencies up to 20%. The modular architecture of this plasmonic material allows the fine-tuning of its photocatalytic properties by simple manipulation of a reduced number of basic components.

    Nationell ämneskategori
    Nanoteknik
    Forskningsämne
    Teknisk fysik med inriktning mot nanoteknologi och funktionella material; Teknisk fysik med inriktning mot nanoteknologi och funktionella material
    Identifikatorer
    urn:nbn:se:uu:diva-328630 (URN)10.1038/s41598-017-09261-7 (DOI)000407864400051 ()
    Forskningsfinansiär
    Stiftelsen för internationalisering av högre utbildning och forskning (STINT), IB2015-6474
    Tillgänglig från: 2017-08-28 Skapad: 2017-08-28 Senast uppdaterad: 2018-12-17Bibliografiskt granskad
    6. Hydrated Electron Generation by Excitation of Localized Surface Plasmons in Copper Nanoparticles
    Öppna denna publikation i ny flik eller fönster >>Hydrated Electron Generation by Excitation of Localized Surface Plasmons in Copper Nanoparticles
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    (Engelska)Ingår i: Science Advances, ISSN 2375-2548Artikel i tidskrift, Dagstidning (Refereegranskat) Submitted
    Nationell ämneskategori
    Naturvetenskap
    Identifikatorer
    urn:nbn:se:uu:diva-369929 (URN)
    Tillgänglig från: 2018-12-17 Skapad: 2018-12-17 Senast uppdaterad: 2018-12-17
  • 2.
    Gupta, Deepesh Kumar
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinsk biokemi och mikrobiologi. Uppsala University.
    Adhesion-dependent mechanisms regulating mitosis2019Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Integrin-mediated cell adhesion is required for normal cell cycle progression during G1-S transition and for the completion of cytokinesis. Cancer cells have ability to grow anchorage-independently, but the underlying mechanisms and the functional significance for cancer development are unclear. The current thesis describes new data on the adhesion-linked molecular mechanisms regulating cytokinesis and centrosomes. 

          Non-adherent fibroblast failed in the last step of the cytokinesis process, the abscission. This was due to lack of CEP55-binding of ESCRT-III and its associated proteins to the midbody (MB) in the intercellular bridge (ICB), which in turn correlated with too early disappearance of PLK1 and the consequent premature CEP55 accumulation. Integrin-induced FAK activity was found to be an important upstream step in the regulation of PLK1 and cytokinetic abscission. Under prolonged suspension culture, the MB disappeared but septin filaments kept the ICB in the ingressed state. Upon re-plating on fibronectin, such cells were found to divide through traction-based abscission. Non-adherent cytokinetic cells maintained septin filaments around the ICB for >24 hours, but septin was gradually depolymerized later on and furrow-regressed binucleated cells were generated (<15%). Binucleated non-transformed cells were halted in G1 and became senescent, possibly via PIDDosome formation by two centrosomes merging. In contrast to normal fibroblasts, ras-transformed fibroblasts were able to recruit the ESCRT-III-associated protein ALIX to MB under non-adherent condition. Live-cell imaging and septin-7 immuno-staining showed that cytokinetic abscission occurred in non-adherent ras-transformed fibroblast.  

          Non-adherent fibroblasts, as well as adherent cells lacking FAK expression, were also delayed in early stages of mitosis progression and exhibited defect centrosome separation and abnormal spindle formation. Our data show that integrin-dependent FAK activity promotes centrosome separation via a FAK-PLK1-Eg5 route during early mitosis.

    Delarbeten
    1. Integrin signaling via FAK-Src controls cytokinetic abscission by decelerating PLK1 degradation and subsequent recruitment of CEP55 at the midbody
    Öppna denna publikation i ny flik eller fönster >>Integrin signaling via FAK-Src controls cytokinetic abscission by decelerating PLK1 degradation and subsequent recruitment of CEP55 at the midbody
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    2016 (Engelska)Ingår i: OncoTarget, ISSN 1949-2553, E-ISSN 1949-2553, Vol. 7, nr 21, s. 30820-30830Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Adhesion to extracellular matrix is required for cell cycle progression through the G1 phase and for the completion of cytokinesis in normal adherent cells. Cancer cells acquire the ability to proliferate anchorage-independently, a characteristic feature of malignantly transformed cells. However, the molecular mechanisms underlying this escape of the normal control mechanisms remain unclear. The current study aimed to identify adhesion-induced reactions regulating the cytokinesis of non-transformed human fibroblasts. The adhesion-dependent control of cytokinesis was found to occur at a late stage close to the abscission, during which the endosomal sorting complex required for transport (ESCRT) severs the thin intercellular bridge connecting two nascent daughter cells. CEP55, a key protein involved in the abscission process, was localized at the midbody in both adherent and non-adherent fibroblasts, but it was unable to efficiently recruit ALIX, TSG101, and consequently the ESCRT-III subunit CHMP4B was missing in the non-adherent cells. PLK1, a kinase that prevents premature recruitment of CEP55 to the midbody, disappeared from this site more rapidly in the non-adherent cells. A FAK-Src signaling pathway downstream of integrin-mediated cell adhesion was found to decelerate both PLK1 degradation and CEP55 accumulation at the midbody. These data identify the regulation of PLK1 and CEP55 as steps where integrins exert control over the cytokinetic abscission.

    Nyckelord
    cytokinesis, CEP55, PLK1, integrin, FAK
    Nationell ämneskategori
    Cancer och onkologi
    Identifikatorer
    urn:nbn:se:uu:diva-299793 (URN)10.18632/oncotarget.9003 (DOI)000377746600074 ()
    Forskningsfinansiär
    Cancerfonden
    Tillgänglig från: 2016-07-28 Skapad: 2016-07-27 Senast uppdaterad: 2018-12-04Bibliografiskt granskad
    2. Tension-induced cytokinetic abscission in human fibroblasts
    Öppna denna publikation i ny flik eller fönster >>Tension-induced cytokinetic abscission in human fibroblasts
    2018 (Engelska)Ingår i: OncoTarget, ISSN 1949-2553, E-ISSN 1949-2553Artikel i tidskrift, Dagstidning (Övrigt vetenskapligt) Published
    Nationell ämneskategori
    Cellbiologi
    Identifikatorer
    urn:nbn:se:uu:diva-368349 (URN)
    Tillgänglig från: 2018-12-04 Skapad: 2018-12-04 Senast uppdaterad: 2018-12-06
    3. Fate of bi-nucleated cells: the role of septin and Ras
    Öppna denna publikation i ny flik eller fönster >>Fate of bi-nucleated cells: the role of septin and Ras
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    2018 (Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
    Abstract [en]

    Integrin-mediated adhesion is required to complete cytokinesis, and failure in the process can generate tetraploid cells, which are potentially oncogenic. The effect of cell detachment on the cytokinesis process and on the following cell cycle was analyzed in the non-transformed human fibroblast cell line BJ and in BJ cells expressing SV40 LT (BJ-LT) +/- an oncogenic Ras mutant. In non-adherent BJ and BJ-LT cells, ALIX could not be recruited to the midbody (MB) and cytokinetic abscission did not occur. Based on the results from several approaches, this block was concluded to be overcome in the detached BJ-LT-Ras cells. Non-adherent BJ and BJ-LT cells maintained the septin-associated intercellular bridge (ICB) formed by cleavage furrow ingression, for more than 24 hours. After re-adhesion to fibronectin most such cells divided by cytofission due to tension exerted on the narrow bridge, while a minor fraction of the cell population instead became bi-nucleated because of regression of the intercellular bridge. Adherent bi-nucleated BJ-LT cells progressed through the cell cycle and at mitosis they divided into two mono-nucleated (4N) cells, while adherent bi-nucleated BJ cells were arrested in the G1 phase and became senescent. Thus, p53-dependent mechanism(s) prevented the formation of tetraploid cells from non-transformed bi-nucleated cells. The two centrosomes in the adherent bi-nucleated cells rapidly fused, indicating that p53 was activated via the PIDDosome mechanism. The results show that several mechanisms contribute to prevent detached normal cells from generating tumor-causing tetraploid cells, and that expression of an activating Ras mutation can promote cytokinesis in detached tumor cells. 

    Nationell ämneskategori
    Cellbiologi
    Identifikatorer
    urn:nbn:se:uu:diva-368333 (URN)
    Tillgänglig från: 2018-12-04 Skapad: 2018-12-04 Senast uppdaterad: 2018-12-04
    4. Focal adhesion kinase (FAK) is required for centrosome separation and subsequent bipolar mitotic spindle  assembly
    Öppna denna publikation i ny flik eller fönster >>Focal adhesion kinase (FAK) is required for centrosome separation and subsequent bipolar mitotic spindle  assembly
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    (Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
    Nationell ämneskategori
    Cellbiologi
    Identifikatorer
    urn:nbn:se:uu:diva-368351 (URN)
    Tillgänglig från: 2018-12-04 Skapad: 2018-12-04 Senast uppdaterad: 2018-12-12
  • 3.
    Skorup, Paul
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Infektionssjukdomar.
    Antibacterial Effect and Inflammatory Response in Relation to Antibiotic Treatment of Sepsis2019Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Sepsis defines as life-threatening organ dysfunction caused by a dysregulated host response to infection. The importance of early administration of antibiotics in septic shock is undisputed, but the optimal antibiotic choice remains uncertain. Some national guidelines advocate single β-lactam antibiotic treatment while others recommend a combination of β-lactam and aminoglycoside. This thesis aimed to investigate the anti-bacterial properties and antibiotic-induced inflammatory responses of ß-lactam antibiotic compared with effects of the addition of an aminoglycoside in clinically relevant E. coli porcine intensive care sepsis/septic shock models. We also studied the host's antibacterial capacities in primary and secondary sepsis.

    In Paper I the addition of an aminoglycoside, in comparison with single β-lactam antibiotic treatment,  caused decreased bacterial growth in the liver and greater antibiotic-induced blood killing activity ex vivo. The results thereby constitute possible mechanisms to the previously reported improved survival in the most critically ill sepsis patients receiving the β-lactam/aminoglycoside combination. Also observed in this paper was that individual blood bactericidal capacity may have significant effects on antimicrobial outcome.  

    In Paper II we investigated endotoxin release in vivo after antibiotic treatment in comparison with no treatment. There were no differences, however, antibiotics did increase an inflammatory IL-6 response that was associated with leukocyte activation and pulmonary organ dysfunction. A secondary finding was that the addition of an aminoglycoside to a β-lactam induced trends towards less inflammation compared with β-lactam alone.

    Paper III compared how challenge with different pre-killed E. coli activates the inflammatory response, resulting in higher cytokine responses, more leucocyte activation and inflammatory capillary leakage after single β-lactam compared with live or heat-killed bacteria. The addition of an aminoglycoside lowered the β-lactam-induced responses.

    Paper IV demonstrated that animals with secondary sepsis exhibited an attenuated inflammatory response as expected; however, contrary to our hypothesis, the animals’ antibacterial capacities were intact and partly enhanced.

    We conclude that there are likely several beneficial effects of the addition of an aminoglycoside to a β-lactam therapy regimen in septic shock. Because host antibacterial capacities in secondary sepsis are enhanced, the need for bactericidal antibiotic combinations is not greater in secondary than in primary sepsis.

    Delarbeten
    1. Beneficial Antimicrobial Effect of the Addition of an Aminoglycoside to a β-Lactam Antibiotic in an E. coli Porcine Intensive Care Severe Sepsis Model.
    Öppna denna publikation i ny flik eller fönster >>Beneficial Antimicrobial Effect of the Addition of an Aminoglycoside to a β-Lactam Antibiotic in an E. coli Porcine Intensive Care Severe Sepsis Model.
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    2014 (Engelska)Ingår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 9, nr 2, s. e90441-Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    This study aimed to determine whether the addition of an aminoglycoside to a ß-lactam antibiotic increases the antimicrobial effect during the early phase of Gram-negative severe sepsis/septic shock. A porcine model was selected that considered each animal's individual blood bactericidal capacity. Escherichia coli, susceptible to both antibiotics, was given to healthy pigs intravenously during 3 h. At 2 h, the animals were randomized to a 20-min infusion with either cefuroxime alone (n = 9), a combination of cefuroxime+tobramycin (n = 9), or saline (control, n = 9). Blood samples were collected hourly for cultures and quantitative polymerase chain reaction (PCR). Bacterial growth in the organs after 6 h was chosen as the primary endpoint. A blood sample was obtained at baseline before start of bacterial infusion for ex vivo investigation of the blood bactericidal capacity. At 1 h after the administration of the antibiotics, a second blood sample was taken for ex vivo investigation of the antibiotic-induced blood killing activity. All animals developed severe sepsis/septic shock. Blood cultures and PCR rapidly became negative after completed bacterial infusion. Antibiotic-induced blood killing activity was significantly greater in the combination group than in the cefuroxime group (p<0.001). Growth of bacteria in the spleen was reduced in the two antibiotic groups compared with the controls (p<0.01); no difference was noted between the two antibiotic groups. Bacterial growth in the liver was significantly less in the combination group than in the cefuroxime group (p<0.05). High blood bactericidal capacity at baseline was associated with decreased growth in the blood and spleen (p<0.05). The addition of tobramycin to cefuroxime results in increased antibiotic-induced blood killing activity and less bacteria in the liver than cefuroxime alone. Individual blood bactericidal capacity may have a significant effect on antimicrobial outcome.

    Nationell ämneskategori
    Medicin och hälsovetenskap
    Identifikatorer
    urn:nbn:se:uu:diva-220397 (URN)10.1371/journal.pone.0090441 (DOI)000332396200200 ()24587365 (PubMedID)
    Tillgänglig från: 2014-03-13 Skapad: 2014-03-13 Senast uppdaterad: 2018-12-12Bibliografiskt granskad
    2. Dynamics of Endotoxin, Inflammatory Variables, and Organ Dysfunction After Treatment With Antibiotics in an Escherichia coli Porcine Intensive Care Sepsis Model
    Öppna denna publikation i ny flik eller fönster >>Dynamics of Endotoxin, Inflammatory Variables, and Organ Dysfunction After Treatment With Antibiotics in an Escherichia coli Porcine Intensive Care Sepsis Model
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    2018 (Engelska)Ingår i: Critical Care Medicine, ISSN 0090-3493, E-ISSN 1530-0293, Vol. 46, nr 7, s. e634-e641Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    OBJECTIVES: To investigate the dynamics of antibiotic-induced endotoxin liberation and inflammatory response in vivo in a clinically relevant large animal intensive care sepsis model and whether the addition of an aminoglycoside to a β-lactam antibiotic affects these responses.

    DESIGN: Prospective, placebo-controlled interventional experimental study.

    SETTING: University research unit.

    SUBJECTS: Thirty-six healthy pigs administered Escherichia coli as a 3-hour infusion.

    INTERVENTIONS: After 2 hours, during E. coli infusion, the animals were exposed to cefuroxime alone, the combination of cefuroxime and tobramycin, or saline.

    MEASUREMENTS AND MAIN RESULTS: Plasma endotoxin, interleukin-6, tumor necrosis factor-α, leucocytes, and organ dysfunction were recorded for 4 hours after antibiotic treatment, and differences to the values before treatment were calculated. In vitro experiments were performed to ascertain whether endotoxin is released during antibiotic-induced bacterial killing of this E. coli strain. Despite differences between the treatment arms in vitro, no differences in plasma endotoxin were observed in vivo. Antibiotic-treated animals demonstrated a higher interleukin-6 response (p < 0.001), greater leucocyte activation (p < 0.001), and more pronounced deterioration in pulmonary static compliance (p < 0.01) over time than controls. Animals treated with the combination showed a trend toward less inflammation.

    CONCLUSIONS: Treatment with antibiotics may elicit an increased inflammatory interleukin-6 response that is associated with leucocyte activation and pulmonary organ dysfunction. No observable differences were detected in plasma endotoxin concentrations. The reduction in cefuroxime-induced endotoxin release after the addition of an aminoglycoside in vitro could not be reproduced in this model.

    Nationell ämneskategori
    Infektionsmedicin
    Identifikatorer
    urn:nbn:se:uu:diva-349226 (URN)10.1097/CCM.0000000000003139 (DOI)000435290400002 ()29595561 (PubMedID)
    Tillgänglig från: 2018-04-23 Skapad: 2018-04-23 Senast uppdaterad: 2018-12-12Bibliografiskt granskad
    3. Mode of Bacterial Killing Affects the Inflammatory Response and Associated Organ Dysfunctions in a Porcine E. coli Intensive Care Sepsis Model
    Öppna denna publikation i ny flik eller fönster >>Mode of Bacterial Killing Affects the Inflammatory Response and Associated Organ Dysfunctions in a Porcine E. coli Intensive Care Sepsis Model
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    (Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
    Nyckelord
    Antibiotics, Bacteria, Sepsis, Cytokines, Inflammation, Porcine
    Nationell ämneskategori
    Medicin och hälsovetenskap
    Identifikatorer
    urn:nbn:se:uu:diva-366974 (URN)
    Tillgänglig från: 2018-11-27 Skapad: 2018-11-27 Senast uppdaterad: 2018-12-12Bibliografiskt granskad
    4. Enhanced Bacterial Clearance at a Secondary Sepsis Challenge in an Endotoxin-tolerant Porcine Intensive Care Model
    Öppna denna publikation i ny flik eller fönster >>Enhanced Bacterial Clearance at a Secondary Sepsis Challenge in an Endotoxin-tolerant Porcine Intensive Care Model
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    (Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
    Nyckelord
    Secondary sepsis, Endotoxin-tolerance, Bacteria, E. coli, Porcine, ICU
    Nationell ämneskategori
    Medicin och hälsovetenskap Infektionsmedicin
    Identifikatorer
    urn:nbn:se:uu:diva-366977 (URN)
    Anmärkning

    P Skorup and F Wilske contributed equally to this work.

    Tillgänglig från: 2018-11-27 Skapad: 2018-11-27 Senast uppdaterad: 2018-12-12Bibliografiskt granskad
  • 4.
    Edvinsson, Åsa
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kv