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  • 101.
    Cederberg, Peter
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Fürstenberg-Hägg, Emil
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Karlsson, Joakim
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Sylvin Majlund, David
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Wellhagen, Gustaf
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Hantering av produktionsbatcher på Spiber Technologies2012Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 102.
    Celma, Gunta
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Nanocellulose hydrogels for topical wound-care applications: a study on human skin interactions2017Självständigt arbete på avancerad nivå (yrkesexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
  • 103.
    Chantzi, Efthymia
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Fast processing of label-free video microscopy movies of human and bacterial cell populations growing in vitro during chemical exposure2016Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
    Abstract [en]

    A fast computational framework for large-scale parallel processing of label-free video microscopy movies of human and bacterial cell populations growing in vitro during chemical exposure was developed in MATLAB®. The overarching aim was to quantify and study time evolving morphological effects due to chemical perturbations caused by single drugs and combinations. Using this framework, a previously reported method for characterization of differences in time evolving morphologies of human cell populations, based on pixel histogram hierarchies of phase-contrast microscopy images, was re-implemented, refined and subsequently optimized with respect to method-specific tuning parameters. This implementation  was also generalized for time-lapse microscopy movies of bacterial cell cultures, generated by the oCelloScope™ system, which acquires multiple series of images of non-adherent cell populations in the cell culture medium. In addition, a separate computational framework for large-scale parallel quantification of the bacterial growth was deployed as an alternative to the growth kinetics analysis provided by the integrated commercial software of the oCelloScope™ system. The potential of the implemented frameworks was demonstrated on experimental data by processing time-lapse movies from different human and bacterial cell populations, while being exposed to different single chemical compounds and combinations. These novel computational tools are compatible with either single high-end multi-core computers or cloud-based distributed computing infrastructures offered via MapReduce, and Hadoop® MapReduce, respectively. This enables fast and fault-tolerant processing of huge video microscopy datasets and opens for optimization of user-defined tuning parameters.

  • 104.
    Che, Huiwen
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Evaluation of de novo assembly using PacBio long reads2016Självständigt arbete på avancerad nivå (magisterexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [en]

    New sequencing technologies show promise for the construction of complete and accurate genome sequences, by a process called de novo assembly that joins reads by overlap to longer contiguous sequences without the need for a reference genome. High-quality de novo assembly leads to better understanding in genetic variations. The purpose of this thesis is to evaluate human genome sequences obtained from the PacBio sequencing platform, which is a new technology suitable for de novo assembly of large genomes. The evaluation focuses on comparing sequence identity between our own de novo assemblies and the available human reference and through that, benchmark accuracy of our data. Sequences that are absent from the reference genome, are investigated for potential unannotated genes coordinately. We also assess the complex structural variation using different approaches. Our assemblies show high consensus with the human reference genome, with ⇠ 98.6% of the bases in the assemblies mapped to the human reference. We also detect more than ten thousand of structural variants, including some large rearrangements, with respect to the reference.

  • 105.
    Chen, Donglei
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för organismbiologi, Evolution och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Squamation in Andreolepis from the late Silurian of Sweden2010Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    The origin of osteichthyans (bony fishes and tetrapods) dates back to the Late Silurian, but theearly evolution of osteichthyans is poorly understood. Andreolepis is one of the oldest knownosteichthyans, but exclusively documented by detached and fragmented dermal microremains.Nevertheless, Andreolepis has unequivocally been attributed to the osteichthyan stem group.A variety of isolated scales of Andreolepis from Gotland, Sweden provides a large dataset,which would potentially improve our understanding of the acquisition of the osteichthyanbody plan. In the present study, various forms of Andreolepis scales were classified into tenmorphotypes by landmark-based geometric morphometrics. Based on comparative anatomyand functional morphology, each morphotype was assigned to a specific area of the body anda squamation model of Andreolepis was established. In this model, scales are allocated toanterior-mid lateral flank scales, posterior lateral flank scales, caudal peduncle scales, pectoralpeduncle scales, dorsal flank scales, dorsal fulcral scales, caudal fulcral scales, ventral flankscales, medioventral scales and cranial scales.

  • 106.
    Chen, Junyu
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Oxygen isotope composition (δ18O) in Sandbar Shark teeth as a proxy to environment: developmental and morphofunctional variability2019Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Oxygen isotope composition is a well-known environmental proxy, studied in both fossil and living organisms. In this research project, the ratio of δ18O was studied in the biomineral of enameloid tissue of Sandbar Shark teeth. All the sharks were kept in the tropical ocean tank of the Blackpool Sea Life Center (UK). The δ18O in 6 lateral teeth from different individuals have been measured by SIMS and had average ratio of about 21‰. 20 teeth of different morphotypes and developmental ages from one individual have been measured by TC-EA MS and showed average δ18O values of 22‰, with no significant difference between the teeth of different morphotypes and developmental ages.

    Publikationen är tillgänglig i fulltext från 2020-05-31 19:54
  • 107.
    Cheng, George
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för ekologi och genetik, Evolutionsbiologi.
    Uncovering the genetic organisation of Claroideoglomus candidum2019Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Arbuscular mycorrhizal (AM) fungi are hypothesized to have been key players in facilitating thetransition from aquatic to terrestrial plants and continue to benefit plants through their symbioticassociation after 450 million years. These fungi form mycelia that can contain hundreds of nucleiwithin one aseptate cytoplasm, which leads to the ongoing debate on whether thesemultinucleated fungi are homokaryotic or heterokaryotic. Not only is there evidence to supportthe hypothesis of the nuclei as genetically identical, but also the other hypothesis of divergentnuclei within a single strain. There has been no evidence of sexual reproduction, howeverspecialized genomic regions specific to meiosis and a putative mating-type (MAT) locus haverecently been identified and may help answer the ongoing debate between homokaryosis andheterokaryosis.

    In this study I applied de novo genome assembly and annotation of 24 individual nuclei from asingle spore of Claroideoglomus candidum. The full length of the de novo genome assembly was87.6 Mb with 17,542 genes. Estimated polymorphism between the nuclei was very low. Iidentified the MAT locus in C. candidum, using a previously sequenced MAT locus fromanother congeneric species. Only one of the MAT locus alleles was found in the examined spore.The evidence points towards homokaryosis as the genetic organization of Claroideoglomuscandidum.

  • 108.
    Cheng, Xi
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Using zebrafish as a model organism to study winner and loser effect2017Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Our past experiences influence the way we cope with things at present. Experience effect is that prior experience which affects the behaviour pattern later on. The outcome of prior agonistic confrontation decides the winner and loser in a sequential fight such that winners have higher probability to win again and losers have higher probability to lose again, which is called winner and loser effects. Winner and loser effects have been described in many animals and studied in detail with the established aggressive model in zebrafish. However, none of the studies considered of individual variation, the personality or behavioural syndromes especially different coping styles such as shy versus bold, proactive versus reactive in zebrafish. Genetic modulation provides us with an option:  spiegeldanio strain (spd) with aggression-boldness syndrome can be used to study the winner loser effect in lab. Pairing spd with wild-type strain (AB) to let them fight with each other and their own mirror images separately can help us understand better about the relationship between personality and winning/losing experience effect and contribute to decoding the molecular mechanism behind this.

  • 109.
    Cholleti, Harindranath
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Paidikondala, Maruthibabu
    Unit for Virology, Department of Virology, Immunobiology and Parasitology of the National Veterinary Institute (SVA).
    Munir, M.
    Hakhverdyan, M.
    Baule, C.
    Equine arteritis virus induced cell death is associated with activation of the intrinsic apoptotic signalling pathway2013Ingår i: Virus Research, ISSN 0168-1702, E-ISSN 1872-7492, Vol. 171, nr 1, s. 222-226Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Equine arteritis virus (EAV) causes a respiratory and reproductive disease in horses, equine viral arteritis. Though cell death in infection with EAV is considered to occur by apoptosis, the underlying molecular mechanism has not been extensively elucidated. We investigated the expression of mRNA of pro-apoptotic and caspase genes during EAV infection in BHK21 cells, a well-established cell type for EAV replication. Using a SYBR Green real-time PCR, mRNA of p53, Bax, caspase 3 and caspase 9 were found up-regulated in a time dependent manner in EAV infected cells. Western blot analysis for caspase 3 and caspase 9 showed expression of cleaved forms of these proteins during EAV infection. In addition, a luminescence-based cell assay for caspase 3/7 activation as a hallmark in apoptosis confirmed apoptotic cell death. The findings demonstrate that cell death in EAV infected BHK21 cells results from apoptosis mediated through the intrinsic signalling pathway.

  • 110.
    Chowath, Rashmi
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Role of Aurora kinase in Medulloblastoma development with correlation to MYCN activity2015Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Brain tumors are abnormal tissue masses found, either malignant or benign in nature. Medulloblastoma is a brain tumor subtype found to arise in the hind region of the brain, which is highly malignant and has poor long term prospects in general. On the basis of the driving force behind the tumor, medulloblastoma is further subgrouped into 4 categories: WNT; SHH; Group 3 and Group 4 tumors. Group 3 tumors show a high expression of N-Myc protein which is seen in certain types of cancerous cells. The cell cycle is regulated at several checkpoints by cyclin/cdk inhibitors. The primary cilium is an organelle found on the cellular surface, which has functions in cell growth, differentiation and neurogenesis. Aurora kinase is a protein kinase involved in the regulation and maintainence of the cilium. Often the cilium gets deleted from the cellular surface in tumors coupled with an increase in the kinase level inside the cells. Hence aurora kinase is found to be a viable target for therapy. Aurora kinase is also involved in stabilizing the MYCN gene by protecting it from degradation.

    In this project, the primary cilum was studied in neural stem cells and followed by study of its presence on tumor cells in culture. The gene involved in cilium development i.e. Kif3a was mutated and its aggressive nature was compared with that of the tumor cells. Aurora kinase was commonly found to be over-expressed in both the tumors and the mutants whereas N-Myc over-expression was seen only in tumors. Experiments suggest that cilia repression in Kif3a mutants takes place via an aurora kinase dependent pathway.

  • 111.
    Cirulis, Aivars
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för ekologi och genetik.
    Separating the sexes: sexual conflict and how to resolve it2016Självständigt arbete på avancerad nivå (magisterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    During the evolution of sex, different sexual conflicts arise. Sexual conflicts reduce fitness of the opposite sex. That is why several mechanisms have evolved to resolve them, which leads to rapid and unpredictable co-evolution of male and female traits involved in reproduction. This rapid co-evolution of male and female reproductive traits driven by sexual conflict can further lead to reproductive isolation resulting in speciation.

                I used the hermaphroditic fungus Neurospora crassa, which has two mating types, as a model organism. Mating types are proxy to sex, because both are needed for sexual reproduction, but they are not limited to either sex role. However by using male pheromone knock-out lines, I created an evolutionary setup, where either mating type is forced to adapt to its restricted sex role. After 21 sexual generations of adaptive co-evolution, I tested if mating types had adapted to the assigned sex by measuring fitness (production of sexual spores called ascospores). I used three evolutionary setups (lines): Δccg4 lines, where mat A is female and mat a is adapted to the male role, Δmfa1 lines, where conversely mat A is adapted to the male role and mat a is female, and wild-type lines used as controls, where both mating types have maintained and adapted to both sex roles. And discovered one Δccg4 line, which indeed adapted to the newly assigned sex roles. At generation 15 and 21 I obtained mixed results for the presence of sexual conflict by correlating male and female fitness in hermaphroditic partner mat a in this line, however I found a sexual conflict also in the asexual growth, where male role is associated with increased, but female role with decreased mycelium growth rate. This work will further allow to study genomic mechanisms underlying this adaptation.

  • 112.
    Clasénius, Jenny
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Sexualundervisning i skolan: En jämförande studie mellan kommunala skolor och kristna friskolor2010Självständigt arbete på avancerad nivå (yrkesexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [sv]

    Sexualundervisningen är en viktig del i skolan, där eleverna får kunskap för livet. Det är därförviktigt att undersöka om alla elever får en likvärdig utbildning, oavsett vilken typ av skola de går i.Syftet med min studie var att belysa hur sexualundervisningen bedrivs i kristna friskolor ochkommunala skolor. I studien deltog två kristna friskolor och två kommunala skolor. För att samlain data användes den kvantitativa metoden enkät och den kvalitativa metoden intervju. Enkätendelades ut till totalt 133 elever och totalt intervjuades sex lärare.Resultatet visade att det är NO-lärarna som har huvudansvaret för sexualundervisningen ibåde de kommunala skolorna och de kristna friskolorna. De kommunala skolorna arbetartvärvetenskapligt oftare än de kristna friskolorna. I de kommunala skolorna pratar de ompornografi, vilket de inte gör i de kristna friskolorna. De kristna friskolorna pratar de, enligteleverna, något mer om hbt-frågor och abort än i de kommunala skolorna. Elevernas upplevelserav sexualundervisningen skiljer sig i frågor om bland annat alla människors lika värde. Eleverna ide kommunala skolorna anser i högre utsträckning att läraren har påtalat alla människors likavärde, än eleverna i de kristna friskolorna. Alla elever borde få möjlighet att diskutera pornografi,då pornografi kan påverka elevernas beteende. Om undervisningen är tvärvetenskaplig, fåreleverna en tydligare helhetsbild. För att utveckla undervisningen är det viktigt att lärarna fårmöjlighet att samtala med andra lärare.

  • 113.
    Connaghan, Daniel
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    An investigation into trait differentiation within and between two closely related Silene species.2017Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Ecological differentiation and adaptation are processes that can drive divergence and speciation. Measuring ecologically revenant traits can help to identify possible targets of natural selection that may have mediated ecological differentiation. This study looked for evidence of within and between species differentiation in seven ecologically relevant traits in two closely related species sampled across their range, and whether any of these traits were related to climate differences among site of origin.

    We measured seven traits under common garden conditions in seedlings of Silene dioica (11 populations, n=528) and Silene latifolia (14 populations, n=672) grown in the botanical garden in Uppsala in a randomised block design. Three traits related to leaf morphology were measured, and four related to water usage of the plant were measured. These traits were analysed for differences between the species as well as for differences within each species between populations using a linear mixed model. The traits’ relationship to a climate variable, derived from the axes of a principal components analysis of climate data for each site, was investigated using a linear model.

    A number of drought avoidance (e.g. succulence, max turgid weight) and morphological traits (e.g. leaf ratio) differed between the two species. Water use efficiency has been flagged before as possibly driving ecological differentiation between the two species and the results identify possible candidate traits for quantifying this separation. Differentiation between populations within each species was also present in two traits within S. latifolia and in four traits within S. dioica significantly varying between the populations. This could reflect either local adaptation or genetic drift acting on populations across the range. One trait related to the amount of water taken up by the leaf (wgain) was found to be significantly associated with the climate variable, which was extracted from the principal components analysis, in S. latifolia.

    The results identified a number of candidate traits which could reflect ecological differences between the species especially with respect to water relations. These traits may allow the species to respond differently during periods of water stress, which in turn could result in ecological separation of the species and determine their geographical ranges. 

  • 114.
    Corujo Chaves, Cátia Ifigénia
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning. Science for Life Laboratory (SciLifeLab), Stockholm.
    Co-occurence and co-metabolism of phototrophic and heterotrophic microbial communities in the Baltic Sea2017Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    In the marine water column, microbial autotrophs and heterotrophs coexist forming the beginning of the food chain which sustains the whole biological system. Both groups play a vital role in the microbial loop. Primary producers, such as algae and Cyanobacteria, use available nutrients in the water (and atmospheric CO2) to release energy, in the form of DOM (dissolved organic matter). Heterotrophic bacteria can then use this energy to remineralise recalcitrant and semilable organic matter present in the water column. Interactions between and among these communities are complex and dependent on several factors such as temperature, salinity, availability of nutrients (C, P, N among others). This remineralisation process is possible, in part, because of extracellular enzymes, produced by the microbial community to hydrolyse large molecules (> 600 Da).

    In this study, samples were isolated from the Baltic Sea. Then Illumina Miseq amplicon sequencing and an enzymatic assay were performed, with the objective of identifying the microbial community and determine patterns of co-occurrence as well as access extracellular enzymatic activity (EEA) in those same communities. Several taxa were identified through sequencing. Among them: heterotrophic bacteria (mainly from the Proteobacteria phylum), Cyanobacteria (mainly Synechococcus) and green algae with Trebouxiophyceae as the dominant class. The EEA showed activity consistent with summer Cyanobacterial blooms, with enzyme activity associated with phosphate mineralisation and cellulose degradation.

  • 115.
    Coyle, Krysta M.
    et al.
    Dalhousie Univ, Dept Pathol, Halifax, NS, Canada..
    Murphy, J. Patrick
    Dalhousie Univ, Dept Microbiol & Immunol, Halifax, NS, Canada..
    Vidovic, Dejan
    Dalhousie Univ, Dept Pathol, Halifax, NS, Canada..
    Vaghar-Kashani, Ahmad
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning. Dalhousie Univ, Dept Pathol, Halifax, NS, Canada.
    Dean, Cheryl A.
    Dalhousie Univ, Dept Pathol, Halifax, NS, Canada.;Dalhousie Univ, Dept Microbiol & Immunol, Halifax, NS, Canada..
    Sultan, Mohammad
    Dalhousie Univ, Dept Pathol, Halifax, NS, Canada..
    Clements, Derek
    Dalhousie Univ, Dept Pathol, Halifax, NS, Canada..
    Wallace, Melissa
    Dalhousie Univ, Dept Microbiol & Immunol, Halifax, NS, Canada..
    Thomas, Margaret L.
    Dalhousie Univ, Dept Pathol, Halifax, NS, Canada..
    Hundert, Amos
    Dalhousie Univ, Dept Psychol & Neurosci & Psychiat, Halifax, NS, Canada..
    Giacomantonio, Carman A.
    Dalhousie Univ, Dept Pathol, Halifax, NS, Canada.;Dalhousie Univ, Dept Surg, Halifax, NS, Canada..
    Helyer, Lucy
    Dalhousie Univ, Dept Surg, Halifax, NS, Canada..
    Gujar, Shashi A.
    Dalhousie Univ, Dept Pathol, Halifax, NS, Canada.;Dalhousie Univ, Dept Microbiol & Immunol, Halifax, NS, Canada.;IWK Hlth Ctr, Dept Qual & Syst Performance, Halifax, NS, Canada..
    Lee, Patrick W. K.
    Dalhousie Univ, Dept Pathol, Halifax, NS, Canada.;Dalhousie Univ, Dept Microbiol & Immunol, Halifax, NS, Canada..
    Weaver, Ian C. G.
    Dalhousie Univ, Dept Psychol & Neurosci & Psychiat, Halifax, NS, Canada..
    Marcato, Paola
    Dalhousie Univ, Dept Pathol, Halifax, NS, Canada..
    Breast cancer subtype dictates DNA methylation and ALDH1A3-mediated expression of tumor suppressor RARRES12016Ingår i: OncoTarget, ISSN 1949-2553, E-ISSN 1949-2553, Vol. 7, nr 28, s. 44096-44112Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Breast cancer subtyping, based on the expression of hormone receptors and other genes, can determine patient prognosis and potential options for targeted therapy. Among breast cancer subtypes, tumors of basal-like and claudin-low subtypes are typically associated with worse patient outcomes, are primarily classified as triple-negative breast cancers (TNBC), and cannot be treated with existing hormone-receptor-targeted therapies. Understanding the molecular basis of these subtypes will lead to the development of more effective treatment options for TNBC. In this study, we focus on retinoic acid receptor responder 1 (RARRES1) as a paradigm to determine if breast cancer subtype dictates protein function and gene expression regulation. Patient tumor dataset analysis and gene expression studies of a 26 cell-line panel, representing the five breast cancer subtypes, demonstrate that RARRES1 expression is greatest in basal-like TNBCs. Cell proliferation and tumor growth assays reveal that RARRES1 is a tumor suppressor in TNBC. Furthermore, gene expression studies, Illumina HumanMethylation450 arrays, and chromatin immunoprecipitation demonstrate that expression of RARRES1 is retained in basal-like breast cancers due to hypomethylation of the promoter. Additionally, expression of the cancer stem cell marker, aldehyde dehydrogenase 1A3, which provides the required ligand (retinoic acid) for RARRES1 transcription, is also specific to the basal-like subtype. We functionally demonstrate that the combination of promoter methylation and retinoic acid signaling dictates expression of tumor suppressor RARRES1 in a subtype-specific manner. These findings provide a precedent for a therapeutically-inducible tumor suppressor and suggest novel avenues of therapeutic intervention for patients with basal-like breast cancer.

  • 116.
    Dahlberg, Gunnar
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Implementation and evaluation of a text extraction tool for adverse drug reaction information2010Självständigt arbete på avancerad nivå (magisterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
    Abstract [sv]

    Inom ramen för Världshälsoorganisationens (WHO:s) internationella biverkningsprogram rapporterar sjukvårdspersonal och patienter misstänkta läkemedelsbiverkningar i form av spontana biverkningsrapporter som via nationella myndigheter skickas till Uppsala Monitoring Centre (UMC). Hos UMC lagras rapporterna i VigiBase, WHO:s biverkningsdatabas. Rapporterna i VigiBase analyseras med hjälp av statistiska metoder för att hitta potentiella samband mellan läkemedel och biverkningar. Funna samband utvärderas i flera steg där ett tidigt steg i utvärderingen är att studera den medicinska litteraturen för att se om sambandet redan är känt sedan tidigare (tidigare kända samband filtreras bort från fortsatt analys). Att manuellt leta efter samband mellan ett visst läkemedel och en viss biverkan är tidskrävande.

    I den här studien har vi utvecklat ett verktyg för att automatiskt leta efter medicinska biverkningstermer i medicinsk litteratur och spara funna samband i ett strukturerat format. I verktyget har vi implementerat och integrerat funktionalitet för att söka efter medicinska biverkningar på olika sätt (utnyttja synonymer,ta bort ändelser på ord, ta bort ord som saknar betydelse, godtycklig ordföljd och stavfel). Verktygets prestanda har utvärderats på manuellt extraherade medicinska termer från SPC-texter (texter från läkemedels bipacksedlar) och på biverkningstexter från Martindale (medicinsk referenslitteratur för information om läkemedel och substanser) där WHO-ART- och MedDRA-terminologierna har använts som källa för biverkningstermer. Studien visar att sofistikerad textextraktion avsevärt kan förbättra identifieringen av biverkningstermer i biverkningstexter jämfört med en ordagrann extraktion.

  • 117.
    Dahlberg, Jenny
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Development of a triplex real-time PCR method for detection of Chlamydia pneumoniae, Chlamydia psittaci and Mycoplasma pneumoniae2016Självständigt arbete på avancerad nivå (yrkesexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
  • 118.
    Danish, Benjamin
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    A novel Adenoviral miRNA, a candidate for development of a novel gene therapy startegy2019Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    In 2017, a novel miRNA was found at the MLTU-region of adenoviral genome, termed as MLP-TSS-sRNA. This current study started with performing a series of mutations in the MLP-TSS-sRNA in order to investigate how the MLP-TSS-sRNA as a single stranded small RNA was protected from rapid RNA degradation in transfected cells (in vivo). Since the hairpin structure of this small RNA was considered to be the reason to its high stability, the deletions of nucleotides were occurred inside the complementary region and the loop of the hairpin structure. Three variants of MLP-TSS-sRNAs were therefore transfected into the A549-lung epithelial cancer cell line and measured during times series studies. The results showed that the wild type form of this small RNA has the highest stability. Subsequently, a panel of different synthetic single-stranded RNAs, in which the MLP-TSS-sRNA sequence was modified to target different genes of interest, was used to compare its suppressive efficiency to the more traditional double stranded small interfering RNA “siRNA” or miRNA mimics. To this, the MLP-TSS-sRNA sequence was modified in such a way that it targeted the Dicer mRNA, thus termed as 3s-dicer-miRNA. Successful suppression of the Dicer mRNA as a consequence of using this modified 3s-dicer-miRNA sequence could emphasize that, theoretically, any possible mRNA of interest could be targeted. To express this miRNA inside a host cell, its sequence was incorporated in a CMV-driven plasmid vector system, upstream of the gene encoding for the HDV-ribozyme, which showed to be functional in vitro, but not in vivo. On the other hand, the vector system showed a clear tendency of being functional even in vivo, once it was put into the test by co-transfecting it with a Dicer plasmid inside 293-cells.

  • 119.
    Denbaum, P
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Devil Facial Tumour Disease: The cancer that's raising hell in Tasmania2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Since 1996 a mysterious epidemic has been sweeping across the island of Tasmania, threatening the Tasmanian devil (Sarcophilus harrisii) with extinction. The species is endemic to the island which is part of Australia and lies south of Melbourne. Being endemic to the island the devils are of particular risk of extinction. Not only has the risk of losing the world’s largest extant carnivorous marsupial aroused great interest, but also the disease itself, has received much attention from the world of oncology due to its unusual trait of being a contagious cancer.

  • 120.
    Denbaum, Philip
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Devil Facial Tumor Disease: Cancern som hotar en arts överlevnad2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [sv]

    Tasmanska djävulen (S. harrisii) var fram till sent 90-tal en ohotad art som förekom i stor abundans på ön Tasmanien där arten är endemisk, nu är arten klassad som hotad av IUCN. Anledningen till att arten blivit hotad är en dödlig smittsam cancersjukdom, Devil Facial Tumor Disease (DFTD) som leder till stora sårbildande tumörer på ansikte, nacke och i munnen. Sedan sjukdomen upptäcktes 1996 har den totala populationen av tasmanska djävular minskat med över 50 % och sjukdomen har nått ungefär 75 % av artens habitat. Tumörerna har en karyotyp som skiljer sig en aning från djävularna vilket tyder på att det skett mutationer av en individs genom som sedan har utvecklats till en egen cellinje kapabel att överleva på och genom värden. DFTD smittar genom ett typiskt beteende hos djävularna som brukar kallas för jaw wrestling vilket innebär att de biter varandra i ansiktet. Hypotesen är att vid bett överförs livskraftiga tumörceller från den ena individen till den andra och börjar växa på den nya värden. Denna typ av sjukdom kallas för klonalt transmissibel och är väldigt ovanligt för en cancersjukdom. Utöver forskning för att lära mer om sjukdomen och för att hitta möjliga resistensgener som kan leda till botemedel eller vaccin så sker också bevarandearbete. Det huvudsakliga bevarandearbetet är försäkringspopulationer, små populationer isolerade från sitt vilda habitat och DFTD. En av de största anledningarna till sjukdomens snabba spridning är S. harrisiis låga genetiska diversitet, speciellt i immunsystemet och Histokompatibilitetskomplex (MHC: Major Histocompatability Complex) (Siddle et al. 2007). Trots att forskningen kommit långt på kort tid så finns idag inga vaccin eller botemedel och artens överlevnad är fortfarande hotad.

  • 121.
    Denbaum, Philip
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning. University of Massachusetts at Amherst - Organismic and Evolutionary Biology.
    Visual reactions to auditory stimulus by the jumping spider Phidippus princeps (Araneae, Salticidae)2019Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Jumping spiders (Family Salticidae) are known for their exceptional vision, including color vision and spatial acuity. Salticids use their vision in many behaviors, including predation and courtship. Recently evidence of their ability to sense airborne vibrations, i.e. sound, was published. I used a specialized jumping-spider-specific eyetracker to study the visual reaction of the retinas of the jumping spider Phidippus princeps when exposed to the sound of a predator. I used a generic wasp sound, previously shown to induce a startle response, as stimulus and played it from different directions. The spiders showed strong reactions to the sound stimulus by large increases in retinal movement when exposed to the stimulus, and they showed no habituation to the stimulus over three rounds of exposure. However, I found no indication that the direction of retinal movement corresponded to the location of the sound source. Future experiments may examine whether spiders are primed to search for particular types of images by cross-modal cues such as sound and if they can determine the direction of a sound source.

  • 122. Detta, Nicola
    et al.
    Brown, Toby D.
    Edin, Fredrik K.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Albrecht, Krystyna
    Chiellini, Federica
    Chiellini, Emo
    Dalton, Paul D.
    Hutmacher, Dietmar W.
    Melt electrospinning of polycaprolactone and its blends with poly(ethylene glycol)2010Ingår i: Polymer international, ISSN 0959-8103, E-ISSN 1097-0126, Vol. 59, nr 11, s. 1558-1562Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Melt electrospinning is one aspect of electrospinning with relatively little published literature, although the technique avoids solvent accumulation and/or toxicity which is favoured in certain applications In the study reported, we melt-electrospun blends of poly(epsilon-caprolactone) (PCL) and an amphiphilic diblock copolymer consisting of poly(ethylene glycol) and PCL segments (PEG-block PCL) A custom-made electrospinning apparatus was built and various combinations of instrument parameters such as voltage and polymer feeding rate were investigated Pure PEG-block-PCL copolymer melt electrospinning did not result in consistent and uniform fibres due to the low molecular weight, while blends of PCL and PEG-block-PCL, for some parameter combinations and certain weight ratios of the two components, were able to produce continuous fibres significantly thinner (average diameter of ca 2 mu m) compared to pure PCL The PCL fibres obtained had average diameters ranging from 6 to 33 mu m and meshes were uniform for the lowest voltage employed while mesh uniformity decreased when the voltage was increased This approach shows that PCL and blends of PEG block-PCL and PCL can be readily processed by melt electrospinning to obtain fibrous meshes with varied average diameters and morphologies that are of interest for tissue engineering purposes.

  • 123.
    Dey, Bonoshree
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Evaluation of peptide-receptor radionuclide therapy in breast cancer and neuroblastoma, in combination with the radio-sensitizing drug VIP-1162018Självständigt arbete på avancerad nivå (yrkesexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Cancer being one of the leading causes of death globally, raises an international fight to defeat and finally conquer cancer. It represents more than 200 different diseases that individually have varying symptoms and treatments. The p53-pathway is of great interest as it has shown to be crucial in the formation of cancerous cells. One method is to use peptide-receptor radionuclide therapy to target somatostatin receptors that are overexpressed on cancer cells. The somatostatin-receptor analogue DOTATATE is a peptide that targets these receptors. In this project, the radionuclide (177Lu) is attached to DOTATATE to achieve a localized radiation effect targeting only the tumour cells and not surrounding tissue. In combination with the radiation-sensitizing drug VIP-116, the effect is supposedly enhanced. The findings of this project conclude the specific targeting of 177Lu-DOTATATE and the potential of VIP-116 to act as a radiosensitizer. The combination of both components works more efficiently on some cell lines than others, which emphasizes the need for tailored cancer therapies. 

    Publikationen är tillgänglig i fulltext från 2021-09-25 00:00
  • 124.
    Dey, Bonoshree
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Jenny, Karlsson
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Yacoub, Nicole
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Eklund, Marcus
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Jonsson, Matilda
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Alternativa Detektionsmetoder för Microarrays2016Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
  • 125.
    Dharamshi, Jennah
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Abundant and diverse novel bacteria in the deep-sea.2015Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
  • 126.
    Dillström, Filip
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Patent som källa för att analysera användning och förekomst av per- och polyfluorerade alkylsubstanser (PFAS)2017Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
  • 127. Diuk-Wasser, Maria A.
    et al.
    Liu, Yuchen
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Steeves, Tanner K.
    Folsom-O'Keefe, Corrine
    Dardick, Kenneth R.
    Lepore, Timothy
    Bent, Stephen J.
    Usmani-Brown, Sahar
    Telford, Sam R., III
    Fish, Durland
    Krause, Peter J.
    Monitoring Human Babesiosis Emergence through Vector Surveillance, New England, USA2014Ingår i: Emerging Infectious Diseases, ISSN 1080-6040, E-ISSN 1080-6059, Vol. 20, nr 2, s. 225-231Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Human babesiosis is an emerging tick-borne disease caused by the intraerythrocytic protozoan Babesia microti. Its geographic distribution is more limited than that of Lyme disease, despite sharing the same tick vector and reservoir hosts. The geographic range of B. microti is expanding, but knowledge of its range is incomplete and relies exclusively on reports of human cases. We evaluated the utility of tick-based surveillance for monitoring disease expansion by comparing the ratios of the 2 infections in humans and ticks in areas with varying babesiosis endemicity. We found a close association between human disease and tick infection ratios in long-established babesiosis-endemic areas but a lower than expected incidence of human babesiosis on the basis of tick infection rates in new disease-endemic areas. This finding suggests that babesiosis at emerging sites is underreported. Vector-based surveillance can provide an early warning system for the emergence of human babesiosis.

  • 128.
    Dolfe, Lisa
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Process development for the control of solubility of Affibody® molecules2011Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
    Abstract [en]

    In this study the aim was to optimize the production of the Affibody fusion-protein Z03358-

    ABD094-(S4G)3-IL2 with regard to the amount of soluble protein produced. However,

    problems with reproducibility with this protein and the chosen expression system were

    encountered. Therefore, expression of the His-tagged Affibody His6-(Z05477)2 was

    evaluated using the same expression system as well as expression in another well

    characterized expression system.

    Both target proteins are of therapeutic interest. One of the proteins is an IL2 fusion

    protein (Z03358-ABD094-(S4G)3-IL2) that bind the platelet-derived growth factor receptor β

    (PDGFR-β). PDGF signaling is of interest in cancer treatment where, among other things, the

    effects of PDGF on tumor angiogenesis is researched. The His6-(Z05477)2 protein has a

    classified target but is developed as a therapeutic in the area of inflammation and autoimmune

    disease. Both model proteins are known to be difficult to purify due to low solubility.

    The two E. coli expression systems investigated and compared were BL21(DE3) and

    Lemo21(DE3). The fusion protein Z03358-ABD094-(S4G)3-IL2 was produced in

    BL21(DE3) in inclusion bodies with a yield of 4.95 g/l. An optimized process for the

    expression of His6-(Z05477)2 using BL21(DE3) was developed with a yield of 6.6 g/l soluble

    protein after expression at 30°C for 6 h.

  • 129.
    Doshi, Jiten
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning. ETH Zurich.
    A platform for Chinese hamster ovary (CHO) cell genome engineering2016Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    The production of therapeutic recombinant proteins in heterologous systems has gained significance since the last decade. For recombinant proteins that require post-translational modifications (PTMs), mammalian systems are preferred. Chinese hamster ovary (CHO) cells are the mammalian cells of choice for production of recombinant proteins. This is because of their ability to provide correct protein-folding and post-translational modifications, displaying high productivity at large scale, ability to grow in suspension mode at high densities in a serum-free media, incapable of infection by most viruses and their history of regulatory approvals. There is an established state of the art technology for development of CHO cells for recombinant protein production. This technology relies on random integration of the gene of interest and gene amplification process for obtaining high expressing clones. There is a high degree of clonal heterogeneity and instability observed in the screened clones. To overcome the process of random integration, this report describes a lentivirus based screening for search of stable and high expressing integration sites in CHO cells. The integration sites are identified by using nrLAM-PCR (non-restrictive linear amplification mediated PCR) coupled with high throughput sequencing. Lentivirus are chosen as they preferentially integrate within the coding regions rendering the possibility of obtaining stable and high expressing clones. In addition, lentivirus vector is designed to possess landing pad for recombinase mediated cassette exchange of viral sequence with foreign DNA. The report describes a successful cassette exchange reaction but with low efficiency. Genome engineering technologies such as CRISPR/Cas, TALENs can used for targeted gene insertion at integration sites and thus establishing stable and efficient production of recombinant proteins in CHO cells. Additionally, an approach for designing synthetic promoters based on Ef1α promoter architecture has been shown. Synthetic promoters are useful for expression of multi-gene cassettes as they are short in length and provide comparable expression levels to the native mammalian promoter. 

  • 130.
    Doszyn, Olga
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Sex differences in neuronal differentiation of human stem cells2019Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Sexual dimorphism has been long noted in human neurobiology, apparent most notably in sex-biased distribution of multiple neurological disorders or diseases, from autism spectrum disorder to Parkinson's disease. With the advances in molecular biology, genetics and epigenetics have come into focus as key players in sexually dimorphic neural development; and yet, many studies in the field of neuroscience overlook the importance of sex for the human brain.

    For this project, human embryonic and neural stem cells were chosen for three main reasons. Firstly, they provide an easily obtainable, scalable and physiologically native model for the early stages of development. Secondly, neural stem cells populations are retained within the adult human brain, and are implicated to play a role in cognition and mental illness, and as such are of interest in themselves. Thirdly, stem cell lines are widely used in research, including clinical trials of transplantation treatments, and for this reason should be meticulously examined and characterized.

    Here, the morphology, behaviour, and expression of selected genes in four stem cell lines, two of female and two of male origin, was examined in side-by-side comparisons prior to and during neuronal differentiation using a variety of methods including light microscopy, time-lapse two-photon microscopy, quantitative real-time PCR and immunocytochemistry. The obtained results have shown previously uncharacterised differences between those cell lines, such as a higher rate of proliferation but a slower rate of neuronal differentiation in male cell cultures compared to female cells cultivated in the same conditions, and a sex-biased expression of several markers of neuronal maturation at late stages of differentiation, as well as diverse patterns of expression of X- and Y-linked genes involved in stem cell proliferation and neural development.

  • 131.
    Duc, Anne-Valérie
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    A comparison of the discrete call repertoires of Northeast Atlantic killer whales (Orcinus orca)2011Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Although Icelandic and Norwegian killer whales are thought to have been in contact prior to the collapse of the herring stock in the 1960s, the Northeast Atlantic killer whales currently seem to show high site fidelity. So far, photoidentification data have suggested movement of a few individuals between East Iceland and North Scotland, and two calls have been shown to be shared by the Icelandic and Norwegian populations. Based on previous and newly analysed call samples, the aim of this study was to describe the geographic variation in the vocal repertoire of the Northeast Atlantic killer whales. Recordings have been conducted off Southwest Iceland in the summers 2004, 2008 and 2009 using sound recording tags attached using suction cups (Dtags), a 4-element vertical hydrophone array and a 2-element towed hydrophone array. From the 57 hours of recording analysed, 1742 calls were classified. In total, 56 distinct call categories composed of 35 call types and 31 subtypes were identified. This discrete call repertoire contained less biphonic calls but more calls composed of buzzes and/or clicks than the Norwegian repertoire. The reasons for these differences remain unknown. One Icelandic call subtype was defined as a compound call, a type of call that is common in the Norwegian population. The comparison of the different vocal repertoires of Northeast Atlantic showed four good or likely call matches in herring-eating killer whales (one between Southwest Iceland and Shetland, one between East Iceland and Norway, and two between Shetland and Norway). No matches were found between Southwest Iceland and East Iceland. I suggest that the four shared calls are most likely to have come from a common ancestral pod and have been transmitted through vocal learning. Over time, geographic isolation of the groups induced by changes in the migratory patterns of the herring might have been accompanied by divergence in their call repertoires.

  • 132.
    Dunder, Linda
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Effects of developmental low-dose exposure to bisphenol A on the adipose tissue in juvenile Fischer 344 rats: Alterations of adipocyte cell density and mRNA gene expression2015Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    BACKGROUND: Bisphenol A (BPA) is a high volume production chemical, used to manufacture certain polycarbonate plastics and epoxy resins. It is also a well-known endocrine disrupting chemical that can mimic endogenous hormones in the body. BPA has been suggested to play a role in the development of obesity and metabolic disorders. Humans are constantly exposed to low doses of BPA due to its ubiquity and studies have shown that the developmental period early in life seems to be particularly sensitive. This has raised a discussion about the potential risk for human health and in January 2015 the European Food Safety Authority (EFSA) reduced the tolerable daily intake (TDI) from 50 μg/kg bw/day to a preliminary TDI of 4 μg/kg bw/day.

    OBJECTIVES: The aim of this present study was to investigate effects of developmental low-dose exposure to BPA on the early distribution of fat cells and on the expression of several genes of interest for adipogenesis.

    METHODS: Pregnant Fischer 344 rats were exposed to BPA via their drinking water corresponding to 0.5 µg/kg bw/day or 50 µg/kg bw/day, during gestation and lactation, until weaning. The routes of exposure for the offspring were in utero and via lactation. The pups were sacrificed at 5 weeks of age. The mRNA gene expression for several genes involved in adipocyte differentiation was measured using qPCR, and adipose cell density in the gonadal, inguinal and brown adipose tissue was examined histologically.

    RESULTS: Developmental exposure of 0.5 µg BPA/kg bw/day significantly increased (122 %, p < 0.05) early adipogenesis as demonstrated by adipocyte hyperplasia in the inguinal fat depot in female juvenile Fischer 344 rats. This increase (129 %; p < 0.05) was also seen in the inguinal fat depot in male offspring, but in animals exposed to 50 µg BPA/kg bw/day compared to 0.5 µg BPA/kg bw/day, but not compared with control.

    Moreover, the mRNA expression of numerous genes of interest for adipogenesis was significantly altered in both females and males. Most interesting was the observed downregulation (p<0.05) of the gene expression of AdipoR1 in the inguinal and AdipoR2 in the gonadal fat depot in males exposed to the lower dose compared with control.

    CONCLUSIONS: Developmental low dose exposure to BPA, even a dose eight times lower than the current TDI, induced significant effects in juvenile Fischer 344 rats. The increase in the number of fat cells observed in juvenile rats may mirror a major public health problem in relation to the obesity epidemic. Additionally, several genes associated with adipogenesis were altered in both female and male Fischer 344 rats. The effects of BPA occurred within the range of environmentally relevant levels and humans are constantly exposed to low doses of BPA, which concludes that exposure to substances such as BPA should be carefully examined in the etiology of obesity.

  • 133.
    Dvizac, Nino
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Turismens utsläpp på Gotland2019Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [sv]

    Turism står för höga ekonomiska intäkter samtidigt som den står för höga utsläpp globalt. I studien diskuteras utsläpp från turism med fokus på den svenska ön, Gotland. Eftersom Gotland är en ö, är färdmedel till ön begränsade. Generellt finns det tre sätt att resa till ön, via färja, flygplan eller kryssningsfartyg. Cirka 70% av besökarna reser till ön med färja och är därför i fokus under studien. I studien beräknades turismens utsläpp genom resa, till och från ön samt under vistelsen. Under vistelsen beräknades utsläpp från transport på ön, till olika destinationer samt utsläpp från boende. Resultaten från studien pekar på att en turist på Gotland har mycket lägre växthusgasutsläpp än den genomsnittliga globala turisten. Den största anledningen är de låga utsläppen från svensk elproduktion.

  • 134.
    Dölfors, Fredrik
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Växters effektorutlösta försvars funktionoch evolution: Ett uthålligt skydd mot patogener?2014Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [sv]

    Diversiteten bland gener som ger resistens (R) mot infektionssjukdomar är mycket stor i växtriket, särskilt i den effektorutlösta klassen av försvaret. Denna artikel beskriver interaktioner mellan växters effektorutlösta fösvar och patogeners effektorproteiner och undersöker evolutionära och genetiska mekanismer för uppkomsten av ny resistens. R-genprodukter har en typisk domänstruktur och interagerar både direkt och indirekt med patogeners effektorproteiner.Vid kontakt med ett effektorprotein utlöses en försvarsrespons som kan förhindra fortsatt patogen tillväxt. Flera genetiska mekanismer verkar samtidigt på R-gener, något som resulterar i en hög hastighet för uppkomst av nya R-varianter. Den intima samevolution som existerar hos många patogen-växtsystem formar det evolutionära utvecklingsmönstret av R-gener. Diversifierande positiv selektion via den biologiska kapprustningsmodellen och bevarande negativ frekvensberoende selektion är båda viktiga samevolutionära processer. Denna artikel indikerar att växters immunförsvar är anpassningsbart och robust, men belyser också den kunskapsbrist som råder inom resistensforskningen.

  • 135.
    Eckerström Liedholm, Simon
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Brain size does not affect reproductive behaviour in male guppies (Poecilia reticulata)2014Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    The processes and mechanisms that govern brain size evolution remain a widely discussed topic in evolutionary biology. How relative brain size relates to animal behaviour and cognition is even more controversial. Recent comparative and experimental studies have shown a positive relationship between relative brain size and complexity of behaviour. Some of the most important behaviours that have direct consequences for an individual’s fitness are reproductive behaviours, and they sometimes require quite complex behavioural repertoires. Selection for complex behaviour might therefore induce an expansion of brain size to allow for cognitively demanding tasks during courtship and mating. In the present study we investigated the effect of relative brain size on reproductive behaviour in male guppies (Poecilia reticulata), using fish from a recently established brain size artificial selection experiment. Females were paired with either a large- or a small-brained male, and we collected data on a suite of male courtship behaviours including sneak copulation attempts, courtship display, gonopodial swings and time spent following the female. Although the extent of orange colouration, a trait that varies across large- and small-brained males, affected male behaviour, we were not able to detect any difference in reproductive behaviour between the brain size selection lines. These results suggest that there is no strong association between male mating behaviour and relative brain size, and future studies will examine this question further. But currently, our results indicate that relative brain size might not be linked to reproductive behaviour to any significant extent, at least not in the guppy.

  • 136.
    Edblom, Johan
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Exploration of big data and machine learning in retail2017Självständigt arbete på avancerad nivå (yrkesexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
    Abstract [en]

    During the last couple of years, there has been an immense increase

    in data generation. This new data era has been referred to as the big

    data paradigm. More and more business areas are today realizing the

    power of capturing more data, and by this hope to reveal hidden

    patterns and gain new insights of their business. ICA is one of the

    largest retail business in Sweden, and saw the potential of utilizing

    the big data technologies to take the next step in digitalisation.

    The objective of this thesis is to investigate the role of these

    techniques in combination with machine learning algorithms and

    highlights advantages and possible limitations. Two use cases were

    implemented and tested which reveals possible application areas and

    important aspects to consider.

  • 137.
    Eklöf, Disa
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Survey of mycotoxin producing fungi in goji berries, oil seeds and walnuts on the Swedish market2013Självständigt arbete på avancerad nivå (magisterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
  • 138.
    Ekman, Malin
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Undervisning i autentisk miljö: Skillnader i mängd utomhusundervisning, studiebesök och besökande som kommer till skolor och håller i undervisning2016Självständigt arbete på avancerad nivå (yrkesexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [sv]

    Utomhusundervisning, studiebesök och besökande som kommer till skolan och håller i undervisning, är exempel på undervisning i autentiska miljöer. Sådan undervisning har visat sig hjälpa elever med deras inlärning, när det förekommer i kombination med annan undervisning. Syftet med undersökningen var att ta reda på hur mycket undervisning i autentiska miljöer gymnasielärare i ett län i Sverige hade arrangerat i kurserna i naturkunskap läsåret 15/16. För att göra detta skickades en enkät ut till naturkunskapslärarna och deras rektorer i länet, och två av lärarna intervjuades. Nästan alla svarande lärare arrangerade någon typ av undervisning i autentiska miljöer. Utomhus studerades ofta den omgivande miljön. Vid studiebesök gavs eleverna information som rörde platsen de befann sig på. När besökande kom till skolan och höll i undervisningen undervisade de om hälsa, samt sex- och samlevnad.

  • 139.
    Ekström, Andreas
    et al.
    Univ Gothenburg, Dept Biol & Environm Sci, Box 463, S-40530 Gothenburg, Sweden..
    Jutfelt, Fredrik
    Norwegian Univ Sci & Technol, Dept Biol, Trondheim, Norway..
    Sundström, L. Fredrik
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Adill, Anders
    Swedish Univ Agr Sci, Inst Coastal Res, Dept Aquat Resources, Oregrund, Sweden..
    Aho, Teija
    Swedish Univ Agr Sci, Inst Coastal Res, Dept Aquat Resources, Oregrund, Sweden..
    Sandblom, Erik
    Univ Gothenburg, Dept Biol & Environm Sci, Box 463, S-40530 Gothenburg, Sweden..
    Chronic environmental warming alters cardiovascular and haematological stress responses in European perch (Perca fluviatilis)2016Ingår i: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology, ISSN 0174-1578, E-ISSN 1432-136X, Vol. 186, nr 8, s. 1023-1031Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Environmental warming and acute stress increase cardiorespiratory activity in ectothermic animals like fish. While thermal acclimation can buffer the direct thermal effects on basal cardiorespiratory function during chronic warming, little is known about how acclimation affects stress-induced cardiorespiratory responses. We compared cardiovascular and haematological responses to chasing stress in cannulated wild European perch (Perca fluviatilis) from a reference area at natural temperature (16 A degrees C) with perch from the 'Biotest enclosure'; an experimental system chronically warmed (22 A degrees C) by effluents from a nuclear power plant. Routine blood pressure was similar, but Biotest perch had slightly higher resting heart rate (59.9 +/- 2.8 vs 51.3 +/- 2.9 beats min(-1)), although the Q (10) for heart rate was 1.3, indicating pronounced thermal compensation. Chasing stress caused hypertension and a delayed tachycardia in both groups, but the maximum heart rate increase was 2.5-fold greater in Biotest fish (43.3 +/- 4.3 vs 16.9 +/- 2.7 beats min(-1)). Moreover, the pulse pressure response after stress was greater in reference fish, possibly due to the less pronounced tachycardia or a greater ventricular pressure generating capacity and thermally mediated differences in aortic compliance. Baseline haematological status was also similar, but after chasing stress, the haematocrit was higher in Biotest fish due to exacerbated red blood cell swelling. This study highlights that while eurythermal fishes can greatly compensate routine cardiorespiratory functions through acclimation processes, stress-induced responses may still differ markedly. This knowledge is essential when utilising cardiorespiratory variables to quantify and compare stress responses across environmental temperatures, and to forecast energetic costs and physiological constraints in ectothermic animals under global warming.

  • 140.
    Ekström, Emma
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    SPR-based method for concentration determination of proteins in a complex environment2012Självständigt arbete på avancerad nivå (yrkesexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
    Abstract [en]

    In this project a method based on surface plasmon resonance has been developed for determining the concentration of several His-tagged proteins in complex solutions. It showed large dynamic range, no measureable non-specific binding and high sensitivity (with linear range around 0.1–10 μg/ml depending on the proteins). The method showed a low variation when checked on MBP-His during an extended time period. The concentrations of the His-tagged protein in the lysate has also been determined and compared with other alternative methods. This method will later be used to analyse protein concentrations during development and optimization of chromatographic purification process.

  • 141.
    Elfman, Sara
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Chromosome Y Fetal fraction assay2016Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
  • 142.
    Elgh, Erik
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Pterosaur Ichnites from the Late Jurassic of Poland: Morphometrical comparisons with body fossils2018Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
  • 143.
    Elhorst, Paula
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning. Institute for Medical Biochemistry and Microbiology (IMBIM).
    Regulation of TGFß signalling by the long noncoding RNA TGFß2-AS12018Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Long noncoding RNAs have been shown to regulate many signalling pathways and their expression has been linked to the development of many cancers. Here we assess the involvement of the long noncoding RNA TGFß2-AS1 in the regulation of the TGFß signalling pathway, specifically its involvement in the TGFß induced process of EMT. In this study, we found that TGFß treatment induced the expression of TGFß2-AS1, and furthermore, TGFß2-AS1 has an inhibitory effect on the expression of the TGFß target genes SERPINE1/PAI-1, CDH2/N-cadherin and Fibronectin. Moreover, our data indicates that TGFß2-AS1 expression has a pro-mitotic effect, that is regulated by PRC2-mediated repression of p15, in HaCaT cells. In conclusion, we show that several EMT markers are differentially regulated by TGFß2-AS1 in response to TGFß and that TGFß2-AS1 plays a role in regulating proliferation.

  • 144.
    Elofsson, Hampus
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Immunoassay engineering: An explorative comparison of detection chemestries and surfaces for protein microarrays2016Självständigt arbete på avancerad nivå (yrkesexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
  • 145.
    Engman, Sarah
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Computational analysis of transcriptional regulation in proximal versus distal normal colon2011Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Factors involved in transcriptional regulation in the normal colon were investigated computationally. Genes differentially expressed between distal and proximal colon were identified and their promoter structures were analysed for presence of TATA-boxes, CpG islands and transcription factor binding sites. The CpG methylation status of the genes were experimentally verified in colon cancer cell lines and compared between the two groups ofdifferentially expressed genes. A difference in methylation frequency in colon cancer celllines was detected between genes up-regulated and down-regulated in normal proximal colon.

  • 146.
    Engqvist, Martin
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    A generic capture assay for immunogenicity, using Biacore2013Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
    Abstract [en]

    The purpose of this investigation was to create and optimise a capture assay for the detectionof anti-drug antibodies (ADA) in human plasma, using Biacore. We also dealt with the nonspecificplasma binding to mouse-derived anti-biotin which may occur in the capture assay.By paying attention to these things we aimed at reaching as high sensitivity as possible for theADA detection.

    The capture assay also benefited and gained flexibility from using the same regenerationsolution irrespective of drug and from having a composition that minimises the risk ofdamaging drug epitopes.

  • 147.
    Engström, Mathias
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Pontén, Olle
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Philip, Carlsson
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Bahnam, Nadeen
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Strömberg, Ella
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Westlin, Oskar
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Antibiotic free and optimised protein production using Escherichia coli2018Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Affibody® molecules are small therapeutic proteins which mimics antibody functionality. This is a report of several methods for increasing productivity and yield in recombinant production of Affibody® molecules. This literature study shows several steps in the production line which can be optimised, several novel methods for cultivating and harvesting cells and purication of proteins. There is also a section about validation of therapeutic protein production according to The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) are presented.

  • 148.
    Engzell, Anna
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Destruktion av per- och polyfluorerade alkylsubstanser (PFAS) vid förbränning i avfallsförbränningsanläggningar2019Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Per- and polyfluorinated alkyl substances (PFAS) are a group of environmentally harmful chemicals. The group consists of more than 4730 different substances including two of the most famous ones; PFOA and PFOS. PFAS consist of carbon backbones connected to fluorine. They are in general thermally stable and persistent to degradation due to their C-F bonds, which is one of the strongest bonds in chemistry. The use of PFAS is widespread and therefore they can be found in a variety of consumer products, which eventually end up as waste. Household waste and other waste streams are incinerated at waste incineration facilities at (at least) 850° C for two seconds. The aim of this study was to investigate if that temperature is high enough to, at least partly, break down PFAS and to study where in the outflows PFAS ends up. Three different facilities (B2, P4 and P6) for waste incineration where examined, including incoming fuels; household waste, industrial waste, return fuel and sewage sludge. The three facilities included in the study had two types of boilers (grated and circulating fluidized bed) with three different treatment facilities for the flue gas and condensate. Bottom ash, fly ash, end product and condensate were analysed after incineration. A mass balance based on the limited amount of data indicates that at least 86-98 % of the 11 examined PFAS substances are eliminated. The difference between the three facilities are probably due to a difference in temperature and a few problems during the sample week. Another reason might be the variations in PFAS concentrations in incoming fuels. The facility with the lowest elimination percentage also had the lowest PFAS-concentrations in incoming fuels, and some problems during the sample period. The facility with the highest elimination percentage had a more even and higher temperature.

  • 149.
    Erdélyi, András
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för cell- och molekylärbiologi, Struktur- och molekylärbiologi.
    HisA mutants with minor structural differences display major functional deviations2016Självständigt arbete på avancerad nivå (masterexamen), 40 poäng / 60 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Even though enzymes tend to specialize on one reaction during evolution, enzyme promiscuity is an abundant phenomenon. The subject of this thesis is the Salmonella enterica N’-[(5’-phosphosoribosyl)-formimino]-5-aminoimidazole-4 carboxamide-ribonucleotide (ProFAR) isomerase (SeHisA), a (βα)8-barrel enzyme from the histidine biosynthesis that catalyzes one reaction on one substrate in one organism. In Actinobacteria HisA has evolved to a bifunctional enzyme called phosphoribosyl isomerase A (PriA): it is capable of catalyzing the reaction normally done by the N’-(5’-phosphoribosyl) anthranilate (PRA) isomerase (TrpF) as well. The functional plasticity of PriA is possible due to the common reaction mechanism of HisA and TrpF, called Amadori rearrangement. The Amadori rearrangement is an acid-base catalyzed isomerization reaction where the aminoaldose (ProFAR or PRA) is converted into the corresponding ketose (PRFAR or CdRP). A SeHisA mutant with a glutamine to arginine mutation in position 18 (Q18R) shows a detectable TrpF activity, whereas another mutant with a duplication of residues from 13 to 15 (dup13-15) loses its HisA activity and gains TrpF activity.

    My first aim was to improve the TrpF activity of the Q18R mutant. A G79S mutation was introduced inspired by PriA. Proteins were purified and crystallized. In order to gain complex structures with either the TrpF reaction product analogue reduced CdRP (rCdRP) or ProFAR, co-crystallization and soaking were done. ProFAR is not commercially available and had to be synthetized and purified. In vitro TrpF activities of the Q18R and Q18R/G79S mutants were measured. My second aim was to compare the Q18R mutant with the dup13-15 mutant, since there is very little structural difference between them, but they show high difference in catalytic activity. Mutants, which would bridge the functional gap between them, were designed and by using lambda red recombineering were introduced into a Salmonella typhimurium genome. In vivo growth rate was measured and relative fitness was calculated for each mutant in respect to their HisA and TrpF activity.

    HisA mutants Q18R and Q18R/G79S showed very poor TrpF activity in in vitro assays. No dissociation constants could be measured for either of the mutants using microscale thermophoresis, and a very low kcat/KM value (~2 s-1M-1) with a high error rate could be determined for Q18R/G79S. Complex structures of Q18R and Q18R/G79S mutants with ProFAR were solved at a 2.47 Å and a 1.78 Å, respectively, from soaked crystals. No structure with rCdRP was obtained. Growth rate measurements in comparison with a strain with wild type HisA and TrpF, gave striking results pointing out the important role of the residue in the position 16 when three residues are inserted after the arginine in position 18. A leucine in position 16 yielded wild type HisA activity (94%) and poor TrpF activity (0-5%), with a valine, no HisA activity (0%) and a moderate TrpF activity (38-46%) were found compared to the wild type. These results pointed out how small the barrier between a specialist, a promiscuous and a bifunctional enzyme can be. SeHisA, being a specialized enzyme can easily be modified in order to gain TrpF function, and as I have showed in the present study, a single methyl group (the difference between a leucine and a valine) can turn the activity of an enzyme inside out.

  • 150.
    Eriksson, Alexandra
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    The internationalization of new technology within an organization with unclear ownership2017Självständigt arbete på avancerad nivå (yrkesexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
    Abstract [en]

    This master thesis was done at Uppsala Clinical Research Center (UCR). UCR have recently developed the technical platform QReg 5 for managing quality registries. UCR have been in contact with several international organizations interested in collaborating with UCR because of their expertise within quality registries as well as their technical competence. UCR have a complicated owner structure as well as organizational structure, as they are owned by Uppsala University and Uppsala County Council. The purpose of this master thesis was to present how QReg 5 could be commercialized under UCR's preconditions. In order for the internationalization plans to be successful, it was shown that the shared ownership of UCR needs to become