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  • 151.
    Possnert, Göran
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. Uppsala University, Disciplinary Domain of Science and Technology, För teknisk-naturvetenskapliga fakulteten gemensamma enheter, Tandem Laboratory.
    Kulan, A
    Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences, LUVAL.
    Berggren, Ann-Marie
    Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences, LUVAL.
    Aldahan, Ala
    Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences, Environment and Landscape Dynamics. Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences, LUVAL.
    Beryllium isotopes in aerosols over Sweden2006Conference paper (Refereed)
  • 152. Punning, J-M
    et al.
    Kangur, M
    Koff, T
    Possnert, G
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Holocene lake-level changes and their reflection in paleolimnological records of two lakes in northern Estonia2003In: Journal of Paleolimnology, no 29Article in journal (Refereed)
  • 153.
    Rahman, Mahbubur
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Division for Electricity and Lightning Research.
    Cooray, Vernon
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Division for Electricity and Lightning Research.
    Possnert, Göran
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Nyberg, Johan
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Nuclear and Particle Physics.
    An experimental quantification of the NOx production efficiency of energetic alpha particles in air2006In: Journal of Atmospheric and Solar-Terrestrial Physics, ISSN 1364-6826, E-ISSN 1879-1824, Vol. 68, no 11, p. 1215-1218Article in journal (Refereed)
  • 154.
    Ramström, Margareta
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
    Hagman, Charlotte
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Mitchell, Jennifer
    Derrick, Peter
    Håkansson, Per
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Bergquist, Jonas
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Depletion of High-Abundant Proteins in Body Fluids Prior to Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry2005In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 4, no 2, p. 410-416Article in journal (Refereed)
    Abstract [en]

    Today, proteomics is an exciting approach to discover potential biomarkers of different disorders. One challenge with proteomics experiments is the wide concentration range of proteins in various tissues and body fluids. The most abundant component in human body fluids, human serum albumin (HSA), is present at concentrations corresponding to approximately 50% of the total protein content in e.g., plasma and cerebrospinal fluid (CSF). If this component could be selectively removed, then the chances of observing lower-abundance component of clinical interest would be greatly improved. There are today several approaches of varying specificity available for depletion. In this study, the properties of two commercially available kits, for the removal of HSA and HSA and immunoglobulin G (lgG), respectively, were compared, and the benefits of using depletion steps prior to on-line LC-FTICR MS were evaluated. Both methods were applied on plasma and CSF. To our knowledge, these are the first results reported for CSF. Also, the combination with electrospray LC-FTICR MS is novel. The proportion of depleted HSA and lgG was estimated using global labeling markers for peptide quantification. Both depletion-methods provided a significant reduction of HSA, and the identification of lower abundant components was clearly facilitated. A higher proportion of HSA was removed using the affinity-based removal kit, and consequently more proteins could be identified using this approach.

  • 155.
    Ramström, Margareta
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Hagman, Charlotte
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Tsybin, Youri O
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Markides, Karin E
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Håkansson, Per
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    Salehi, Albert
    Lundqvist, Ingmar
    Håkansson, Rolf
    Bergquist, Jonas
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
    A novel mass spectrometric approach to analysis of hormonal peptides in extracts of mouse pancreatic islets2003In: European Journal of Biochemistry, ISSN 0014-2956, E-ISSN 1432-1033, Vol. 270, no 15, p. 3146-3152Article in journal (Refereed)
    Abstract [en]

    Liquid chromatography mass spectrometry (LC-MS) is a valuable tool in the analysis of proteins and peptides. The combination of LC-MS with different fragmentation methods provides sequence information on components in complex mixtures. In this work, on-line packed capillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation techniques, i.e. nozzle-skimmer fragmentation and electron capture dissociation, for the determination of hormonal peptides in an acid ethanol extract of mouse pancreatic islets. The most abundant peptides, those derived from proinsulin and proglucagon, were identified by their masses and additional sequence-tag information established their identities. Interestingly, the experiments demonstrated the presence of truncated C-peptides, des-(25-29)-C-peptide and des-(27-31)-C-peptide. These novel findings clearly illustrate the potential usefulness of the described technique for on-line sequencing and characterization of peptides in tissue extracts.

  • 156.
    Ramström, Margareta
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Physical and Analytical Chemistry, Analytical Chemistry. Technology, Department of Engineering Sciences, Ion Physics. Analytisk kemi.
    Ivonin, Igor
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Department of Physical and Analytical Chemistry, Analytical Chemistry. Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Johansson, Anders
    Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience. Department of Physical and Analytical Chemistry, Analytical Chemistry. Technology, Department of Engineering Sciences, Ion Physics.
    Askmark, Håkan
    Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience. Department of Physical and Analytical Chemistry, Analytical Chemistry. Technology, Department of Engineering Sciences, Ion Physics.
    Zubarev, Roman
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Department of Physical and Analytical Chemistry, Analytical Chemistry. Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Håkansson, Per
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Department of Physical and Analytical Chemistry, Analytical Chemistry. Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Aquilonius, Sten-Magnus
    Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience. Department of Physical and Analytical Chemistry, Analytical Chemistry. Technology, Department of Engineering Sciences, Ion Physics.
    Bergquist, Jonas
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry. Department of Physical and Analytical Chemistry, Analytical Chemistry. Technology, Department of Engineering Sciences, Ion Physics. Analytisk kemi.
    Cerebrospinal fluid protein patterns in neurodegenerative disease revealed by liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry2004In: Proteomics, no 4, p. 4010-4018Article in journal (Refereed)
    Abstract [en]

    This study demonstrates the power of a novel proteomic approach developed for the detection and identification of biological markers in body fluids. The goal was to observe alterations in the protein patterns of cerebrospinal fluid (CSF) related to amyotrophic lateral sclerosis (ALS), a neuro-degenerative disorder with unknown etiology. In the experiments, tryptic digests of CSF from patients and healthy controls were analyzed by on-line capillary liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Typically, around 4000 peptides were detected in one such experiment, and a pattern recognition program was constructed for the data analysis to distinguish mass chromatograms from patients and controls. This strategy was evaluated comparing the peptide patterns of CSF spiked in vitro with a biomarker, with control CSF. The patterns were clearly separated and the tryptic peptides of the biomarker were successfully selected as characteristic peaks. Hence, the method was applied to compare mass chromatograms of CSF from 12 ALS-patients and 10 matched healthy controls. While no biomarker alone could be identified from the characteristic peaks, we were able to assign 4 out of 5 unknown samples correctly (i.e. 80% correctly diagnosed, 20% false-negative), and it would be 100% if we reject a possible outlier believed to be caused by an occlusion in the spinal CSF compartment. These findings are very promising, although the clinical relevance is not fully established due to the low number of unknown samples analyzed. In addition to the diagnostic potential, these results may be important steps towards understanding the neurodegenerative process.

  • 157.
    Ramström, Margareta
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
    Palmblad, Magnus
    Jonfysik. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
    Markides, Karin E
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
    Håkansson, Per
    Jonfysik. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
    Bergquist, Jonas
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
    Protein identification in cerebrospinal fluid using packed capillary liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry2003In: Proteomics, Vol. 3, no 2, p. 184-190Article in journal (Refereed)
    Abstract [en]

    The identification and characterization of proteins in complex biological samples such as body fluids, require powerful and reliable tools. Mass spectrometry is today one of the most important methods in such research. This paper reports on the results from the first experiment where a tryptic digest of cerebrospinal fluid was analyzed applying reversed phase liquid chromatography coupled on-line to a 9.4 T Fourier transform ion cyclotron resonance mass spectrometer. In total, 70 204 peaks were detected, which originated from 16 296 isotopic clusters corresponding to 6551 unique peptide masses. From these masses, 39 proteins were identified in the sample. The amount of sample required for one experiment corresponds to 32 μL of cerebrospinal fluid.

  • 158.
    Razpet, Alenka
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Physics, Department of Physics and Materials Science, Materials Science. Chemistry, Department of Materials Chemistry, Inorganic Chemistry. Jonfysik.
    Johansson, Anders
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Physics, Department of Physics and Materials Science, Materials Science. Chemistry, Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Possnert, Göran
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Physics, Department of Physics and Materials Science, Materials Science. Chemistry, Department of Materials Chemistry, Inorganic Chemistry. Jonfysik.
    Skupinski, Marek
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Physics, Department of Physics and Materials Science, Materials Science. Chemistry, Department of Materials Chemistry, Inorganic Chemistry. Materialvetenskap.
    Hjort, Klas
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Physics, Department of Physics and Materials Science, Materials Science. Chemistry, Department of Materials Chemistry, Inorganic Chemistry. Materialvetenskap.
    Hallén, Anders
    Fabrication of high-density ordered nanoarrays in silicon dioxide by MeV ion track lithography2005In: Journal of Applied Physics, Vol. 97, p. 044310-Article in journal (Refereed)
    Abstract [en]

    Self-assembled nanoporous alumina films were employed as masks for MeV ion track lithography. Films with thickness of 2 µm and pore diameters of 30 and 70 nm were attached to thermally grown SiO2 covered with a thin gold layer. The samples were aligned with respect to the beam by detecting backscattered He+ ions with the initial energy of 2 MeV. The ordered pattern of the porous alumina films was successfully transferred into SiO2 after irradiation with a 4 MeV Cl2+ beam at fluence of 1014 ions/cm2, followed by chemical etching in a 5% HF solution.

  • 159.
    Razpet, Alenka
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Possnert, Göran
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Johansson, A
    Abid, M
    Hallén, Anders
    Properties of ordered nanoporous alumina as a template for pattern transfer by MeV ion irradiation2004In: Mat. Res. Soc. Symp Proc, Vol. 792, p. 575-580Article in journal (Refereed)
  • 160.
    Razpet, Alenka
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Physics, Department of Physics and Materials Science, Materials Science. Technology, Department of Engineering Sciences, Ion Physics. Chemistry, Department of Materials Chemistry, Inorganic Chemistry. jonfysik.
    Possnert, Göran
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Physics, Department of Physics and Materials Science, Materials Science. Technology, Department of Engineering Sciences, Ion Physics. Chemistry, Department of Materials Chemistry, Inorganic Chemistry. jonfysik.
    Johansson, Anders
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Physics, Department of Physics and Materials Science, Materials Science. Technology, Department of Engineering Sciences, Ion Physics. Chemistry, Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Hallén, Anders
    Hjort, Klas
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Physics, Department of Physics and Materials Science, Materials Science. Technology, Department of Engineering Sciences, Ion Physics. Chemistry, Department of Materials Chemistry, Inorganic Chemistry. Materialvetenskap.
    Ion transmission and characterization of ordered nanoporous alumina2004In: Nuclear Instruments and Methods Physics Research, Vol. B, no 222, p. 593-600Article in journal (Refereed)
    Abstract [en]

    Ordered nanoporous alumina samples with a pore diameter of 70 nm, an array period of 100 nm and several thicknesses were considered as possible masks for pattern transfer by MeV ion lithography. A simple procedure for the sample alignment using a 2 MeV He+ beam was utilized. The energy distributions of transmitted ions as well as backscattering spectra were studied in aligned and non-aligned orientations. The best transmission, comparable to the relative surface area covered by pores, was reached for 2 um thick samples and was independent on ion species. Although the transmission for thicker mambranes was generally lower, it significantly depended on the quality of each individual sample. The presented ion beam technique can therefore be used as a tool for the characterization of porous materails. The acceptance angle for transmission through pores and the effective atomic density of samples can be obtained from the experimental data and it is shown that nanoporous alumina can be used as a mask for MeV ion lithography.

  • 161.
    Salehpour, Mehran
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Forsgard, Niklas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Possnert, Goran
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Accelerator mass spectrometry of small biological samples2008In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 22, no 23, p. 3928-3934Article in journal (Refereed)
    Abstract [en]

    Accelerator mass spectrometry (AMS) is an ultra-sensitive technique for isotopic ratio measurements. In the biomedical field, AMS can be used to measure femtomolar concentrations of labeled drugs in body fluids, with direct applications in early drug development such as Microdosing. Likewise, the regenerative properties of cells which are of fundamental significance in stem-cell research can be determined with an accuracy of a few years by AMS analysis of human DNA. However, AMS nominally requires about 1 mg of carbon per sample which is not always available when dealing with specific body substances such as localized, organ-specific DNA samples. Consequently, it is of analytical interest to develop methods for the routine analysis of small samples in the range of a few tens of mu g. We have used a 5 MV Pelletron tandem accelerator to study small biological samples using AMS. Different methods are presented and compared. A 12 C-carrier sample preparation method is described which is potentially more sensitive and less susceptible to contamination than the standard procedures.

  • 162.
    Salehpour, Mehran
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Forsgård, Niklas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Possnert, Göran
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    FemtoMolar measurements using accelerator mass spectrometry2009In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 23, no 5, p. 557-563Article in journal (Refereed)
    Abstract [en]

    Accelerator mass spectrometry (AMS) is an ultra-sensitive analytical  method suitable for the detection of sub-nM concentrations of labeled   biological substances such as pharmaceutical drugs in body fluids. A  limiting factor in extending the concentration measurements to the   sub-pM range is the natural C-14 content in living tissues. This was  circumvented by separating the labeled drug from the tissue matrix,  using standard high-performance liquid chromatography (HPLC)   procedures. As the separated total drug amount is in the few fg range,  it is not possible to use a standard AMS sample preparation method,  where mg sizes are required. We have utilized a sensitive carbon carrier method where a C-14-deficient compound is added to the HPLC fractions and the composite sample is prepared and analyzed by AMS.   Using 50 mu L human blood plasma aliquots, we have demonstrated concentration measurements below 20 JFM, containing sub-amol amounts of   the labeled drug. The method has the immediate potential of operating in the sub-fM region.

  • 163.
    Salehpour, Mehran
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Possnert, Göran
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Bryhni, H
    Palminger-Hallén, I
    Ståhle, Lars
    Biological Accelerator Mass Spectrometry at Uppsala University2009In: Applied Radiation and Isotopes, ISSN 0969-8043, E-ISSN 1872-9800, Vol. 67, no 3, p. 495-499Article in journal (Refereed)
    Abstract [en]

    A new research programme for the biological applications of accelerator   mass spectrometry has been initiated at Uppsala University and the   first results are presented. A C-14-labelled pharmaceutical substance   has been dissolved in human blood, plasma and urine and diluted over 3   orders of magnitude. The measured drug concentrations were found to be   in good agreement with the predicted values. Furthermore, the effect of   the sample preparation background contribution has been studied as the   sample amount was varied down to sub-mu l sizes.

  • 164.
    Salehpour, Mehran
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Possnert, Göran
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Bryhni, Heige
    Subattomole sensitivity in biological accelerator mass spectrometry2008In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 80, no 10, p. 3515-3521Article in journal (Refereed)
    Abstract [en]

    The Uppsala University 5 MV Pelletron tandem accelerator has been used to study C-14-labeled biological samples utilizing accelerator mass spectrometry (ANIS) technology. We have adapted a sample preparation method for small biological samples down to a few tens of micrograms of carbon, involving among others, miniaturizing of the graphitization reactor. Standard AMS requires about 1 mg of carbon with a limit of quantitation of about 10 amol. Results are presented for a range of small sample sizes with concentrations down to below 1 pM of a pharmaceutical substance in human blood. It is shown that C-14-labeled molecular markers can be routinely measured from the femtomole range down to a few hundred zepto-mole (10(-21) mol), without the use of any additional separation methods.

  • 165. Santacruz, C.P.
    et al.
    Håkansson, Per
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Barofsky, D. F.
    Piyadasa, C. K. G.
    A Constant-Momentum/Energy-Selector Time-of-Flight Mass Spectrometer2007In: Journal of the American Society for Mass Spectrometry, ISSN 1044-0305, E-ISSN 1879-1123, Vol. 18, no 1, p. 92-101Article in journal (Refereed)
    Abstract [en]

    A matrix assisted laser desorption/ionization time-of-flight mass spectrometer has been built with an ion source that can be operated in either constant-energy or constant-momentum acceleration modes. A decreasing electric field distribution in the ion-accelerating region makes it possible to direct ions onto a space-focal plane in either modes of operation. Ions produced in the constant-momentum mode have velocities and, thus, flight times that are linearly dependent on mass and kinetic energies that are inversely dependent on mass. The linear mass dispersion doubles mass resolving power of ions accelerated with space-focusing conditions in constant-momentum mode. The mass-dependent kinetic energy is exploited to disperse ions according to mass in a simple kinetic energy filter constructed from two closely spaced, oblique ion reflectors. Focusing velocity of ions of the same mass can substantially improve ion selection for subsequent post source decay or tandem time-of-flight analyses.

  • 166. Sanz, R
    et al.
    Hernández-Vélez, M
    Kubart, Tomas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Electronics.
    Martin, David
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Electronics.
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Magnetic behaviour of Fe implanted TiO2 thin films2008In: Book of Abstracts, Madrid, Spain, 2008Conference paper (Refereed)
  • 167. Sanz, R.
    et al.
    Jaafar, M.
    Asenjo, A.
    Rosa, W.O.
    Hernandez-Vélez, M.
    Badini-Confalonieri, G.A.
    Vázquez, M.
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Effects on the Structural and Magnetic Properties of Amorphous Ribbons caused by 4 MeV Cl^2+ ion irradiations2006Conference paper (Other academic)
  • 168. Sanz, R
    et al.
    Jaafar, M
    Hernández-Vélez, M
    Asenjo, A
    Vásquez, M
    Surpi, A
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Magnetic patterns induced by restricted ion irradiation through different masks2008In: Book of Abstracts, Forth Worth, USA, 2008Conference paper (Refereed)
  • 169.
    Sanz, R.
    et al.
    Instituto de Ciencia de Materiales de Madrid.
    Jaafar, M.
    Instituto de Ciencia de Materiales de Madrid.
    Rosa, W.O.
    Instituto de Ciencia de Materiales de Madrid.
    Badini-Confalonieri, G.
    Instituto de Ciencia de Materiales de Madrid.
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Hernandez-Vélez, M.
    Instituto de Ciencia de Materiales de Madrid.
    Vázquez, M.
    Instituto de Ciencia de Materiales de Madrid.
    Effects on the structural and magnetic properties of amorphous ribbons of (Co0.94Fe0.06)(72.5)Si12.5B15 caused by 4 MeV Cl2+ ion irradiation2007In: Journal of Non-Crystalline Solids, ISSN 0022-3093, E-ISSN 1873-4812, Vol. 353, no 8-10, p. 879-882Article in journal (Refereed)
    Abstract [en]

    The use of energetic ion irradiation to modify magnetic materials has attracted increasing interest in recent years. The possibility of patterning surfaces on these materials offers a wide range of potential applications particularly in technologies related to magnetic storage media, sensing devices and electromagnetic shielding materials. In this work, ultrasoft non-magnetostrictive (Co0.94Fe0.06)72.5Si12.5B15 amorphous ribbons, 50 μm thick and 0.85 mm wide, fabricated by the chilly block melt spinning technique are irradiated, in their amorphous state, by 4 MeV Cl2+ ions with a fluence of 5 × 1013 cm-2. The hysteresis properties of both irradiated and non-irradiated samples are characterized by means of a vibrating sample magnetometer while surface magnetic domain structure is observed by Bitter technique. The presence of an induced magnetic anisotropy in irradiated samples is ascribed to the local damage, caused by ion irradiation treatment, which results in modified coercive field and permeability of the samples. X-ray diffraction results are presented to confirm the amorphicity of the structure even after irradiation with ions.

  • 170.
    Sanz, Ruy
    et al.
    Material Science Institute of Madrid.
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Johansson, Anders
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry.
    Skupinski, Marek
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Materials Science. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Possnert, Göran
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Materials Science. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Boman, Mats
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry.
    Hernandez-Velez, Manuel
    Vazquez, Manuel
    Hjort, Klas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Well-ordered nanopore arrays in rutile TiO2 single crystals by swift heavy ion-beam lithography2007In: Nanotechnology, ISSN 0957-4484, E-ISSN 1361-6528, Vol. 18, no 30, p. 305303-Article in journal (Refereed)
    Abstract [en]

    Ion track lithography has been applied for transferring the self-ordered nanopattern of porous anodic alumina to single-crystalline rutile TiO2 substrates. As a result, nanometre resolved arrays have been fabricated with an aspect ratio ranging from 5 to 16, over areas of several square millimetres. Differences in the expected aspect ratio of the resulting nanopores in rutile TiO2 single crystals are analysed and discussed. Some of these differences may be ascribed to varying densities of the mask material.

  • 171. Sanz, Ruy
    et al.
    Johansson, Anders
    Skupinski, Marek
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Materials Science.
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Possnert, Göran
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Boman, Mats
    Vázquez, Manuel
    Hjort, Klas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Fabrication of Well-Odered High-Aspect-Ratio Nanopore Arrays in TiO2 Single Crystals2006In: Nano Letters, Vol. 6, p. 1065-1068Article in journal (Refereed)
  • 172. Sanz, Ruy
    et al.
    Johansson, Anders
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Skupinski, Marek
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Materials Science. Materialvetenskap.
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Possnert, Göran
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Boman, Mats
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry, Inorganic Chemistry. oorganisk kemi.
    Vázquez, Manuel
    Hjort, Klas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Materials Science. Materialvetenskap.
    Fabrication of Well-Ordered High-Aspect-Ratio Nanopore Arrays in TiO2 Single Crystals2006In: Nano Letters, Vol. 6, no 5, p. 1065-1068Article in journal (Refereed)
    Abstract [en]

    In this work a successful method for producing high-aspect-ratio nanopatterned single-crystal TiO2 is presented. The method used is based on nanolithography involving swift heavy ion bombardment through a porous anodic alumina mask. Nanopatterning of large areas allows for fabrication of new devices, for example, photonic crystals and electrodes for energy storage and conversion. Crystalline TiO2 also presents optimal characteristics for optical and catalysis applications. Samples were irradiated by MeV Br7+ ions with fluencies ranging from 7.9 × 1013 to 1.2 × 1015 cm-2. The high-energy Br7+ ions induce latent tracks of amorphous material into the TiO2 crystal suitable for selective etching by hydrofluoric acid. High-aspect-ratio (16) nanopatterned areas, up to 4 mm2, were obtained in a single radiation spot onto single-crystalline TiO2.

  • 173.
    Sapota, Tomasz
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences, Air and Water Science. Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences, Environment and Landscape Dynamics. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Håkanson, Lars
    Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences, Air and Water Science. Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences, Environment and Landscape Dynamics. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. LUVA.
    Aldahan, Ala
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. ELD.
    Possnert, Göran
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Sediment flux to Lake Baikal (Siberia, Russia): Modeling approach2006In: Geomorphology, Vol. 80, p. 105-113Article in journal (Refereed)
  • 174.
    Savitski, Mikhail
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Kjeldsen, Frank
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Nielsen, Mikael
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Zubarev, Roman
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Complementary sequence preferences of electron-capture dissociation and vibrational excitation in fragmentation of polypeptide polycations2006In: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 45, no 32, p. 5301-5303Article in journal (Refereed)
    Abstract [en]

    (Chemical Equation Presented) Complementary cleavage: A large-scale (ca. 15000 spectra) comparison between the cleavage sites and the preferences of collision-activated dissociation (CAD) and electron-capture dissociation (ECD) in tandem mass spectrometry has proved beyond doubt their complementary nature (see picture, red: high, blue: low). The study has also suggested the presence of a preferred N-terminal structure in gas-phase tryptic peptide dications.

  • 175.
    Savitski, Mikhail M
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. jonfysik.
    Ivonin, Igor A
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. jonfysik.
    Nielsen, Michael L
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. jonfysik.
    Zubarev, Roman A
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. jonfysik.
    Tsybin, Youri O
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. jonfysik.
    Håkansson, Per
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. jonfysik.
    Shifted-basis technique improves accuracy of peak position determination in Fourier transform mass spectrometry.2004In: J Am Soc Mass Spectrom, ISSN 1044-0305, Vol. 15, no 4, p. 457-61Article in journal (Refereed)
  • 176.
    Savitski, Mikhail M.
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Nielsen, Michael L.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Zubarev, Roman A.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    ModifiComb: New proteomics tool for mapping substochiometric post-translational modifications, finding novel types of modifications and fingerprinting complex protein mixtures.2006In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 5, no 5, p. 935-948Article in journal (Refereed)
    Abstract [en]

    A major challenge in proteomics is to fully identify and characterize the post-translational modification (PTM) patterns present at any given time in cells, tissues, and organisms. Here we present a fast and reliable method ("ModifiComb") for mapping hundreds types of PTMs at a time, including novel and unexpected PTMs. The high mass accuracy of Fourier transform mass spectrometry provides in many cases unique elemental composition of the PTM through the difference DeltaM between the molecular masses of the modified and unmodified peptides, whereas the retention time difference DeltaRT between their elution in reversed-phase liquid chromatography provides an additional dimension for PTM identification. Abundant sequence information obtained with complementary fragmentation techniques using ion-neutral collisions and electron capture often locates the modification to a single residue. The (DeltaM, DeltaRT) maps are representative of the proteome and its overall modification state and may be used for database-independent organism identification, comparative proteomic studies, and biomarker discovery. Examples of newly found modifications include +12.000 Da (+C atom) incorporation into proline residues of peptides from proline-rich proteins found in human saliva. This modification is hypothesized to increase the known activity of the peptide.

  • 177. Schmidt, H.T.
    et al.
    Jensen, Jens
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Reinhed, P.
    Schuch, R.
    Stöchkel, K.
    Zettergren, H.
    Cederquist, H.
    Recoil-ion momentum distributions for transfer ionization in fast proton-He collisions2005In: Phys. Rev, Vol. A72Article in journal (Refereed)
  • 178. Scott, E.M
    et al.
    Bryant, C.
    Carmin, I
    Cook, G.
    Gulliksen, S
    Harkness, D.
    Heinemeier, J.
    McGee, E.
    Naysmith, P.
    Possnert, Göran
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    van der Plicht, H.
    van Strydonck, M.
    Precision and accuracy in applied 14C dating:: Some findings from the Fourth International Radiocarbon Inter-comparison2004In: Jour Archaeoli Sci, no 31, p. 1209-1213Article in journal (Refereed)
  • 179. Scott, M.
    et al.
    Boaretto, E.
    Bryan, C.
    Cook, G.
    Gulliksen, S.
    Harkness, D.
    Heinemeier, J.
    McGee, E.
    Naysmith, P.
    Possnert, Göran
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Future needs and requirements for AMS 14C standard and reference materials2004In: Nuclear instr Meth in Phys Res Section B: Beam interaction with materials and atoms, Vol. 223-224, p. 382-387Article in journal (Refereed)
  • 180. Serre, A.
    et al.
    Langaney, A.
    Tescher-Nicola, M.
    Paunovic, M.
    Possnert, Göran
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Pääbo, S.
    No evidence for neadertal mtDNA contribution to early modern humans2004In: Public Librery of Science, Vol. 2, p. 313-317Article in journal (Refereed)
  • 181.
    Silivra, Oleg A
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Materials Science. Department of Engineering Sciences, Ion Physics. Ion physics.
    Kjeldsen, Frank
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Materials Science. Department of Engineering Sciences, Ion Physics. Ion physics.
    Ivonin, Igor A
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Materials Science. Department of Engineering Sciences, Ion Physics. Ion physics.
    Zubarev, Roman A
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Materials Science. Department of Engineering Sciences, Ion Physics. Ion physics.
    Electron capture dissociation of polypeptides in a three-dimensional quadrupole ion trap: Implementation and first results.2005In: J Am Soc Mass Spectrom, ISSN 1044-0305, Vol. 16, no 1, p. 22-7Article in journal (Refereed)
  • 182. Sjunneskog, Charlotte
    et al.
    Scherer, Reed
    Aldahan, Ala
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Earth Sciences, Department of Earth Sciences. Technology, Department of Engineering Sciences, Ion Physics. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Earth Sciences, Department of Earth Sciences, Environment and Landscape Dynamics. ELD.
    Possnert, Göran
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Technology, Department of Engineering Sciences, Ion Physics. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Earth Sciences, Department of Earth Sciences, Environment and Landscape Dynamics. jonfysik.
    10Be in Antarctica marine and glacial sediment2005In: 10th International conference on accelerator mass spectrometry, Berkeley, 2005, p. 33-Conference paper (Refereed)
  • 183.
    Skupinski, Marek
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences. Materialvetenskap.
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Johansson, A
    Possnert, Göran
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Boman, Mats
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry, Inorganic Chemistry. Oorganisk kemi.
    Hjort, Klas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Materials Science.
    Ion track nanolithography2006Conference paper (Refereed)
  • 184.
    Skupinski, Marek
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Micro Structural Technology.
    Johansson, Anders
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry.
    Jarmar, Tobias
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Razpet, Alenka
    Hjort, Klas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Boman, Mats
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry, Inorganic Chemistry.
    Possnert, Göran
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Carbon nanopillar array deposition on SiO2 by ion irradiation through a porous alumina template2007In: Vacuum, ISSN 0042-207X, E-ISSN 1879-2715, Vol. 82, no 3, p. 359-362Article in journal (Refereed)
    Abstract [en]

    A method for producing ordered carbon nanopillar arrays is presented. The method is based on ion irradiation through a small anodic porous alumina membrane, resulting in carbon deposition within the centers of the pores on a gold covered SiO2/Si substrate. Samples were irradiated by 4 MeV Cl2+ ions with fluences of . The combined use of pick’n place positioning of the small porous alumina templates and ion beam irradiation is well suited for post-processing on silicon based integrated circuits. It provides fast local deposition at low temperature of high-density ordered carbon nanopillar arrays in larger silicon based systems, e.g., for field emitting or biosensors applications.

  • 185.
    Skupinski, Marek
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Physics, Department of Physics and Materials Science, Materials Science. Technology, Department of Engineering Sciences, Ion Physics.
    Razpet, A
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Physics, Department of Physics and Materials Science, Materials Science. Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Johansson, A
    Possnert, G
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Physics, Department of Physics and Materials Science, Materials Science. Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Hjort, Klas
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Physics, Department of Physics and Materials Science, Materials Science. Technology, Department of Engineering Sciences, Ion Physics.
    Hallén, A
    Ordered nanopore arrays in silicon dioxide by ion track projection lithography2005In: 6th Int. Symp. On Swift Heavy Ions in Matter, SHIM (May 28-31, 2005, Aschaffenburg, Germany) A-65., 2005Conference paper (Refereed)
  • 186.
    Skupinski, Marek
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Sanz, Ruy
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Surface Patterning by Heavy Ion Lithography Using Self-Assembled Colloidal Masks2007In: Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, ISSN 0168-583X, E-ISSN 1872-9584, Vol. 257, no 1-2, p. 777-781Article in journal (Refereed)
    Abstract [en]

    Heavy ion lithography using self-assembled colloidal particles as a mask enables micro- and nano-patterning of surfaces. The resulting patterns can be tuned by varying the mask configuration, i.e. packing geometry of the colloidal particles and number of particle layers. In this work we present several patterns, which can be transferred to rutile TiO2 single crystals by irradiating through self-assembled layers of silica micro-spheres with 25 MeV Br ions. As the induced ion tracks in TiO2 have a very high etching selectivity the patterns can be developed in HF with very high contrast. This makes it possible to prepare large patterned areas which can be of interest for e.g. optical applications.

  • 187.
    Skupiński, Marek
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Johansson, Anders
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry.
    Possnert, Göran
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Materials Science.
    Boman, Mats
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry.
    Hjort, Klas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Razpet, Alenka
    Nanopattern transfer to SiO2 by ion track lithography and highly selective HF vapor etching2007In: Journal of Vacuum Science & Technology B, ISSN 1071-1023, E-ISSN 1520-8567, Vol. 25, no 3, p. 862-867Article in journal (Refereed)
    Abstract [en]

    The authors present a method for high aspect ratio nanopatterning of high density (1010  pores/cm2) self-assembled porous alumina membrane pattern into thermally grown SiO2 on silicon. The pattern transfer is accomplished by irradiating through 2  µm thick porous alumina membrane with swift heavy ions (4  MeV Cl2+). Ions passing through the nanopores in the mask at a fairly high fluence (typically 1014  ions/cm2) are impinging on the substrate and creating a continuous volume of overlapping ion tracks of damage. The damage is sufficient to be selectively etched by HF vapor from an aqueous HF solution. From an alumina mask with pores of 70  nm diameter, a pattern of pores of 77  nm in diameter and the same distance of 100  nm between the centers of the pores was transferred. The deepest observed etched pores were 355  nm, giving an aspect ratio of 5, which is up to 40 times larger compare to earlier work where HF wet etching was used. This ion track lithography technique shows a potential to produce nanostructures with even higher aspect ratios.

  • 188. Snyders, R.
    et al.
    Music, D.
    Sigumonrong, D.
    Schelnberger, B.
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Schneider, J.M.
    Experimental and ab initio study of the mechanical properties of hydroxyapatite2007In: Applied Physics Letters, ISSN 0003-6951, E-ISSN 1077-3118, Vol. 90, no 19, p. 193902-Article in journal (Refereed)
    Abstract [en]

    The authors have studied the elastic properties of radio frequency sputtered phase pure, stoichiometric, and dense hydroxyapatite films by nanoindentation. The measured elastic modulus values have been compared to ab initio calculated data. The calculation technique was based on the determination of all elastic constants. The calculated and measured elastic modulus values differ by ∼10%. The good agreement indicates that the elasticity of hydroxyapatite can be described using ab initio calculations, establishing the elastic modulus thereof.

  • 189. Snyders, Rony
    et al.
    Bousser, Etienne
    Music, Denis
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Hocquet, Stephane
    Schneider, Jochen M.
    Influence of the chemical composition on the phase constitution and the elastic properties of RF sputtered hydrozyapatite coatings2008In: Plasma Processes and Polymers, ISSN 1612-8850, Vol. 5, no 2, p. 168-174Article in journal (Refereed)
    Abstract [en]

    We have studied the influence of chemical composition on the constitution and elastic properties of dense radio-frequency (RF)-sputtered hydroxyapatite (HA) coatings. The chemical composition was modified by varying the RF sputtering power density (P-D). As the P-D was increased by 240%, the Ca/P ratio measured by X-ray photoelectron spectroscopy increased from approximate to 1.51 to approximate to 1.82. X-ray diffraction indicates phase pure hexagonal HA except for the sample prepared at the highest P-D where CaO and Ca-3(PO4)(2) also form. Deviations from the stoichiometric Ca/P ratio result in reduction of the elastic modulus. For Ca/P = 1.51 +/- 0.02, the elastic modulus decreases by approximate to 15%. This maybe due to incorporation of Ca vacancies in the lattice, while for Ca/P = 1.82 +/- 0.02, the average elastic modulus decreases by approximate to 10% due to formation of additional phases.

  • 190.
    Spohr, Reimar
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Materials Science, Materials Physics.
    Sharma, Gunjana
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Forsberg, Pontus
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Karlsson, Mikael
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
    Hallén, Anders
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Westerberg, Lars
    Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Materials Science.
    Stroke Asymmetry of Tilted Superhydrophobic Ion Track Textures2010In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 26, no 9, p. 6790-6796Article in journal (Refereed)
    Abstract [en]

    The stroke asymmetry of contact tingles of water drops on tilted hydrophobic textures is demonstrated, obtained by ion track etching followed by a hydrophobic treatment. Preliminary trends concerning the advancing and receding contact angles are established, each with and against stroke direction. In rough agreement with Cassie-Baxter theory, the cosines of these four contact angles depend linearly on the wetted area fraction. The etched tracks are randomly distributed on the surface of polycarbonate disks and inclined by 300 with respect to the surface, whereby the aspect ratio of individual etched cones is larger than 10. The morphology of the resulting surface is characterized by randomly shaped flat tops overhanging on one side and gradually falling off on the other side. The area fraction of the supporting tops can be calculated from the number of impinging ions per unit area and the cross section of the etched ion tracks. The top layer of the texture consists of flat, horizontal, irregularly shaped tops supporting water drops in the Cassie-Baxter state. With increasing etching time, the texture becomes increasingly defied. To fabricate the textures, we irradiated polyearbonate with 5 x 10(7) Br-80(7+) ions/cm(2) of 30 MeV total energy (having a range of about 20 mu m in polycarbonate) at a tilt angle of 30 with respect to the sample surface and etched the latent ion tracks selectively. The textured surface is made hydrophobic by carbondifluoride radicals (CF2) resulting from the decay of octafluorocyclobutane, C4F8, in a plasma reactor. The goal of the report is to show that the tilt orientation of a superhydrophobic surface leads to advancing and receding contact angles depending on the orientation with and against the stroke direction. In addition, a rotating movement is demonstrated qualitatively by floating a rotationally asymmetric disk on an ultrasonic bath, similarly treated after an irradiation with (1.2 +/- 0.4) x 10(7) Xe-129(274) MeV/cm- of 8.3 MeV/nucleon at an angle of 45 degrees, whereby the superhydrophobic side of the disk points downward to the water of the ultrasonic bath.

  • 191. Stöchkel, K.
    et al.
    Eidem, O.
    Cederquist, H.
    Zettergren, H.
    Reinhed, P.
    Schuch, R.
    Cocke, C. L.
    Levin, S. B.
    Ostrovsky, V. N.
    Källberg, A.
    Simonsson, A.
    Jensen, Jens
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Schmidt, H. T.
    Two-center interference in fast proton-H2-electron transfer and excitation processes2005In: Phys. Rev, Vol. A87, p. 050703-Article in journal (Refereed)
  • 192. Svensk, O
    et al.
    Törmä, P T
    Suihkonen, S
    Ali, M
    Lipsanen, H
    Sopanen, M
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Hjort, Klas
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Micro Structural Technology.
    Reduced photoluminescence from InGaN/GaN multiple quantum well structures irradiated by low fluence high energy heavy ions2008In: Book of Abstracts, Phalaborwa, South Africa, 2008Conference paper (Refereed)
  • 193. Tomita, S
    et al.
    Andersen, J.U.
    Cederquist, H.
    Concina, B.
    Huber, B.A.
    Hvelplund, P.
    Jensen, Jens
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Liu, B.
    Manil, B.
    Maunoury, L.
    Brøndsted Nielsen, S.
    Rangama, J.
    Schmidt, H.T.
    Zettergren, H.
    Lifetimes of C_60^2- and C_70^2- dianions in a storage ring2006In: Journal of Chemical Physics, Vol. 124, p. 024310-Article in journal (Refereed)
  • 194. Tonkov, Spassimir
    et al.
    Bozilova, Elissaveta
    Possnert, Göran
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Velcev, Angel
    A contribution to the postglacial vegetation history of the Rila Mountains, Bulgaria: The pollen record of Lake Trilistnika2008In: Quaternary International, ISSN 1040-6182, E-ISSN 1873-4553, Vol. 190, no 1, p. 58-70Article in journal (Refereed)
    Abstract [en]

    Pollen analysis of sediments from the glacial Lake Trilistnika (2216 m) in the Northwestern Rila Mountains (Bulgaria), supplemented by 13 radiocarbon dates, allowed the reconstruction of the palaeoenvironment and vegetation history in postglacial time. The exact time of the cirque glacier retreat is still under discussion but the lake was free of ice before 15,000 cal. BP. when sedimentation of gray silt began. The lateglacial vegetation, composed of Artemisia, Chenopodiaceae and Poaceae, with isolated stands of Pinus and Juniperus-Ephedra shrubland, dominated during the stadials and partly retreated during the Bolling/Allerod interstadial complex. The afforestation in the early Holocene (11,500-7800 cal. BP) started with the distribution of pioneer Benda forests with groups of Pinus (P. mugo, P. sylvestris and P. peuce) at mid-high altitudes, and Quercus forests with Tilia, Ulmus, Fraxinus, Corylus below the birch zone. The change to more humid and cooler climate ca. 7800-7500 cal. BP favored the vertical migration of Abies, P. sylvestris and P. peuce. The establishment of Fagus sylvatica took place after 5200 cal. BP, when pure or mixed beech communities were formed. The last tree which invaded the coniferous belt between 4300 and 3400 cal. BP was Picea abies. The first expansion maximum of spruce was recorded after ca. 2700 cal. BP. The vegetation development in historical times was also influenced by human interference, indicated by the continuous presence of pollen anthropogenic indicators Such as Triticum, Secale, Hordeum, Plantago lanceolata, Rumex, Scleranthus, Juniperus.

  • 195. Tonkov, Spassimir
    et al.
    Possnert, Göran
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Bozilova, Elissaveta
    The lateglacial vegetation and radiocarbon dating of lake Trilistnika, Rila Mountains (Bulgaria)2006In: Vegetation History and Archaeobotany, ISSN 0939-6314, E-ISSN 1617-6278, Vol. 16, no 1, p. 15-22Article in journal (Refereed)
    Abstract [en]

    Pollen analysis, supplemented by 5 C-14 dates, was carried out on the lateglacial section (160 cm) of a core retrieved from the glacial Lake Trilistnika (2216 m) in the northwestern Rila Mountains (Bulgaria). The reconstruction of the lateglacial vegetation history is linked for the first time to a chronological framework for the time window 13000-10000 B.P. The delimitation of an interstadial/stadial cycle, analogous with the Bolling/Allerpd-Younger Dryas from Western Europe, is based on important changes in the pollen stratigraphy. Mountain-steppe vegetation composed of Artemisia, Chenopodiaceae, Poaceae and other cold-resistant herbs, with isolated stands of Pinus and Juniperus-Ephedra shrubland, dominated the landscape after the ice-retreat. Interstadial conditions were established before 12815 +/- 130 B.P. The spread of tree vegetation at lower elevation as a response to the climate improvement was confined to the time interval 12110 +/- 95-11140 +/- 75 B.P. The Younger Dryas stadial is characterised by a re-advance of the mountain-steppe vegetation. The results are compared with other sites of lateglacial age from the high mountains (Rila, northern Pirin, western Rhodopes) in southern Bulgaria.

  • 196.
    Tsybin, Youri
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    High Rate Electron Capture Dissociation Fourier Transform Ion Cyclotron Resonance Mass Spectrometry2004Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Advances in science and technology during the past decade have greatly enhanced the level of the structural investigation of macromolecules – peptides and proteins. Biological mass spectrometry has become one of the most precise and sensitive techniques in peptide and protein analysis. However, increasing demands of biotechnological applications require further progress to be made.

    In the present thesis the development and improvement of peptide and protein characterization methods and techniques based on ion-electron and ion-photon reactions in electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry are described. The focus is on the development of the electron capture dissociation method, recently discovered by the group of professor McLafferty, into a high rate, efficient tandem mass spectrometrical technique.

    The rate and reliability of the electron capture dissociation technique were greatly increased by implementation of low-energy pencil electron beam injection systems based on indirectly heated dispenser cathodes. Further implementation of a hollow electron beam injection system combined, in a single experimental configuration, two rapid fragmentation techniques, high rate electron capture dissociation and infrared multiphoton dissociation. Simultaneous and consecutive irradiations of trapped ions with electrons and photons extended the possibilities for ion activation/dissociation reaction schemes and lead to improved peptide and protein characterization. Using these improvements, high rate electron capture dissociation was employed in time-limited experiments, such as liquid chromatography–tandem mass spectrometry and capillary electrophoresis-tandem mass spectrometry.

    The analytical applications of the developed techniques have been demonstrated in top-down sequencing of peptides and proteins up to 29 kDa, improved sequencing of peptides with multiple disulfide bridges and secondary fragmentation (w-ion formation), as well as extended characterization of peptide mixtures separated by liquid chromatography and capillary electrophoresis. For instance, the dissociation of peptides resulting from enzymatic digestion of proteins provided complementary structural information on peptides and proteins, as well as their post-translational modifications.

    List of papers
    1. Peptide and Protein Characterization by High Rate Electron Capture Dissociation Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
    Open this publication in new window or tab >>Peptide and Protein Characterization by High Rate Electron Capture Dissociation Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
    Show others...
    2004 (English)In: Journal of Mass Spectrometry, ISSN 1076-5174, E-ISSN 1096-9888, Vol. 39, no 7, p. 719-729Article in journal (Refereed) Published
    Abstract [en]

    The analytical utility of the electron capture dissociation (ECD) technique, developed by McLafferty and co-workers, has substantially improved peptide and protein characterization using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). The limitations of the first ECD implementations on commercial instruments were eliminated by the employment of low-energy electron-injection systems based on indirectly heated dispenser cathodes. In particular, the ECD rate and reliability were greatly increased, enabling the combination of ECD/FTICR-MS with on-line liquid separation techniques. Further technique development allowed the combination of two rapid fragmentation techniques, high-rate ECD and infrared multiphoton dissociation (IRMPD), in a single experimental configuration. Simultaneous and consecutive irradiations of trapped ions with electrons and photons extended the possibilities for ion activation/dissociation and led to improved peptide and protein characterization. The application of high-rate ECD/FTICR-MS has demonstrated its power and unique capabilities in top-down sequencing of peptides and proteins, including characterization of post-translational modifications, improved sequencing of peptides with multiple disulfide bridges and secondary fragmentation (w-ion formation). Analysis of peptide mixtures has been accomplished using high-rate ECD in bottom-up mass spectrometry based on mixture separation by liquid chromatography and capillary electrophoresis. This paper summarizes the current impact of high-rate ECD/FTICR-MS for top-down and bottom-up mass spectrometry of peptides and proteins.

    Keywords
    Posttranslational modification, Mass spectrometry, Ion cyclotron resonance spectrometry, Fourier transformation, Electron capture, Protein, Peptides
    National Category
    Chemical Sciences
    Identifiers
    urn:nbn:se:uu:diva-91521 (URN)10.1002/chin.200523298 (DOI)
    Available from: 2004-03-25 Created: 2004-03-25 Last updated: 2017-12-14Bibliographically approved
    2. Improved Low-Energy Electron Injection Systems for High Rate Electron Capture Dissociation in Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
    Open this publication in new window or tab >>Improved Low-Energy Electron Injection Systems for High Rate Electron Capture Dissociation in Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
    Show others...
    2001 (English)In: Rapid Communications in Mass Spectrometry, Vol. 15, p. 1849-Article in journal (Refereed) Published
    Identifiers
    urn:nbn:se:uu:diva-91522 (URN)
    Available from: 2004-03-25 Created: 2004-03-25 Last updated: 2009-03-26Bibliographically approved
    3. Combined Infrared Multiphoton Dissociation and Electron Capture Dissociation with a Hollow Electron Beam in Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
    Open this publication in new window or tab >>Combined Infrared Multiphoton Dissociation and Electron Capture Dissociation with a Hollow Electron Beam in Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
    Show others...
    2003 (English)In: Rapid Communications in Mass Spectrometry, Vol. 17, p. 1759-Article in journal (Refereed) Published
    Identifiers
    urn:nbn:se:uu:diva-91523 (URN)
    Available from: 2004-03-25 Created: 2004-03-25 Last updated: 2009-03-26Bibliographically approved
    4. Electron Capture Dissociation Fourier Transform Ion Cyclotron Resonance Mass Spectrometry in the Electron Energy Range 0-50 eV
    Open this publication in new window or tab >>Electron Capture Dissociation Fourier Transform Ion Cyclotron Resonance Mass Spectrometry in the Electron Energy Range 0-50 eV
    2004 (English)In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 18, no 14, p. 1607-1613Article in journal (Refereed) Published
    Abstract [en]

    Electron capture dissociation (ECD) of polypeptide cations was obtained with pencil and hollow electron beams for both sidekick and gas-assisted dynamic ion trapping (GADT) using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) with an electrostatic ion transfer line. Increasing the number of trapped ions by multiple ICR trap loads using GADT improved the ECD sensitivity in comparison with sidekick ion trapping and ECD efficiency in comparison with single ion trap load by GADT. Furthermore, enhanced sensitivity made it possible to observe ECD in a wide range of electron energies (0-50 eV). The degree, rate and fragmentation characteristics of ECD FTICR-MS were investigated as functions of electron energy, electron irradiation time, electron flux and ion trapping parameters for this broad energy range. The results obtained show that the rate of ECD is higher for more energetic (>1 eV) electrons. Long electron irradiation time with energetic electrons reduces average fragment ion mass and decreases efficiency of formation of c- and z-type ions. The obtained dependencies suggest that the average fragment ion mass and the ECD efficiency are functions of the total fluence of the electron beam (electron energy multiplied by irradiation time). The measured electron energy distributions in low-energy ECD and hot ECD regimes are about 1 eV at full width half maximum in employed experimental configurations.

    National Category
    Chemical Sciences
    Identifiers
    urn:nbn:se:uu:diva-91524 (URN)10.1002/rcm.1525 (DOI)15282786 (PubMedID)
    Available from: 2004-03-25 Created: 2004-03-25 Last updated: 2017-12-14Bibliographically approved
    5. Liquid Chromatography and Electron Capture Dissociation in Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
    Open this publication in new window or tab >>Liquid Chromatography and Electron Capture Dissociation in Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
    Show others...
    2002 (English)In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 16, no 10, p. 988-992Article in journal (Refereed) Published
    Abstract [en]

    Liquid separation methods in combination with electrospray mass spectrometry as well as the recently introduced fragmentation method electron capture dissociation (ECD) have become powerful tools in proteomics research. This paper presents the results of the first successful attempts to combine liquid chromatography (LC) and Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS) with ECD in the analysis of a mixture of standard peptides and of a bovine serum albumin tryptic digest. A novel electron injection system provided conditions for ECD sufficient to yield extensive sequence information for the most abundant peptides in the mixtures on the time-scale of the chromatographic separation. The results suggest that LC/ECD-FTICRMS can be employed in the characterization of peptides in enzymatic digests of proteins or protein mixtures and identify and localize posttranslational modifications.

    National Category
    Analytical Chemistry Subatomic Physics
    Identifiers
    urn:nbn:se:uu:diva-89817 (URN)10.1002/rcm.667 (DOI)000175539300014 ()
    Available from: 2002-04-23 Created: 2002-04-23 Last updated: 2017-12-14
    6. Capillary Electrophoresis and Electron Capture Dissociation Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Peptide Mixture and Protein Digest Analysis
    Open this publication in new window or tab >>Capillary Electrophoresis and Electron Capture Dissociation Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Peptide Mixture and Protein Digest Analysis
    Show others...
    2002 (English)In: European journal of mass spectrometry, ISSN 1469-0667, E-ISSN 1751-6838, Vol. 8, no 5, p. 389-395Article in journal (Refereed) Published
    National Category
    Physical Sciences
    Identifiers
    urn:nbn:se:uu:diva-91526 (URN)10.1255/ejms.514 (DOI)
    Available from: 2004-03-25 Created: 2004-03-25 Last updated: 2017-12-14Bibliographically approved
    Download full text (pdf)
    FULLTEXT01
  • 197.
    Tsybin, Youri O.
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Håkansson, Per
    Budnik, Bogdan A.
    Haselmann, Kim F.
    Kjeldsen, Frank
    Gorshkov, Michael
    Zubarev, Roman A.
    Improved Low-Energy Electron Injection Systems for High Rate Electron Capture Dissociation in Fourier Transform Ion Cyclotron Resonance Mass Spectrometry2001In: Rapid Communications in Mass Spectrometry, Vol. 15, p. 1849-Article in journal (Refereed)
  • 198.
    Tsybin, Youri O.
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Håkansson, Per
    Wetterhall, Magnus
    Markides, Karin E.
    Bergquist, Jonas
    Capillary Electrophoresis and Electron Capture Dissociation Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Peptide Mixture and Protein Digest Analysis2002In: European journal of mass spectrometry, ISSN 1469-0667, E-ISSN 1751-6838, Vol. 8, no 5, p. 389-395Article in journal (Refereed)
  • 199.
    Tsybin, Youri O.
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Ramström, Margareta
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
    Witt, Matthias
    Baykut, Gökhan
    Håkansson, Per
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
    Peptide and Protein Characterization by High Rate Electron Capture Dissociation Fourier Transform Ion Cyclotron Resonance Mass Spectrometry2004In: Journal of Mass Spectrometry, ISSN 1076-5174, E-ISSN 1096-9888, Vol. 39, no 7, p. 719-729Article in journal (Refereed)
    Abstract [en]

    The analytical utility of the electron capture dissociation (ECD) technique, developed by McLafferty and co-workers, has substantially improved peptide and protein characterization using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). The limitations of the first ECD implementations on commercial instruments were eliminated by the employment of low-energy electron-injection systems based on indirectly heated dispenser cathodes. In particular, the ECD rate and reliability were greatly increased, enabling the combination of ECD/FTICR-MS with on-line liquid separation techniques. Further technique development allowed the combination of two rapid fragmentation techniques, high-rate ECD and infrared multiphoton dissociation (IRMPD), in a single experimental configuration. Simultaneous and consecutive irradiations of trapped ions with electrons and photons extended the possibilities for ion activation/dissociation and led to improved peptide and protein characterization. The application of high-rate ECD/FTICR-MS has demonstrated its power and unique capabilities in top-down sequencing of peptides and proteins, including characterization of post-translational modifications, improved sequencing of peptides with multiple disulfide bridges and secondary fragmentation (w-ion formation). Analysis of peptide mixtures has been accomplished using high-rate ECD in bottom-up mass spectrometry based on mixture separation by liquid chromatography and capillary electrophoresis. This paper summarizes the current impact of high-rate ECD/FTICR-MS for top-down and bottom-up mass spectrometry of peptides and proteins.

  • 200.
    Tsybin, Youri O
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Ramström, Margareta
    Chemistry, Department of Chemistry. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Analytisk kemi.
    Witt, Matthias
    Baykut, Gökhan
    Håkansson, Per
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences, Ion Physics. Jonfysik.
    Peptide and protein characterization by high-rate electron capture dissociation fourier transform ion cyclotron resonance mass spectrometry2004In: J. Mass Spectrom, no 39, p. 719-729Article in journal (Refereed)
12345 151 - 200 of 247
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