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  • 151.
    Liu, Haipeng
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Functional Studies of Some Immune Relevant Genes in a Crustacean2008Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    The freshwater crayfish, Pacifastacus leniusculus, mounts a strong innate immune response against microbes such as viruses and bacteria. In this thesis, a novel RNA interference (RNAi) method mediated with histone H2A was developed and applied in crayfish hematopoietic tissue cell cultures for gene functional studies. Further, the interactions between host (crayfish) and pathogens (white spot syndrome virus and Aeromonas hydrophila, respectively) were studied using RNAi technology in live animals. An antilipopolysaccharide factor isolated from viral challenged crayfish by suppression subtractive hybridization was shown to interfere with the propagation of white spot syndrome virus both in vivo and in vitro in crayfish, suggesting an important role of this factor in antiviral defense. Besides, RNAi of phenoloxidase, a critical immune effector involved in melanization, revealed that phenoloxidase activity is necessary for crayfish immune defense against a highly pathogenic bacterial infection in crayfish. In addition, RNAi was also employed to study a marker protein gene involved in hemocyte maturation in crayfish. Taken together, these studies may provide more insights into the immune responses against pathogen invasion as well as hemocyte ontogenesis in crustaceans.

    Delarbeten
    1. Histone H2A as a transfection agent in crayfish hematopoietic tissue cells
    Öppna denna publikation i ny flik eller fönster >>Histone H2A as a transfection agent in crayfish hematopoietic tissue cells
    2007 (Engelska)Ingår i: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 31, nr 4, s. 340-346Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    We report a novel and highly efficient dsRNA transfection system based on one of the nuclear proteins, namely, histone H2A. RT-PCR semi-quantitative analysis of silencing target gene shows that the transfection efficiency of histone H2A is higher than Effectene or liposome-based transfection systems. Importantly, the high efficiency of histone H2A was associated with very low toxicity to the transfected crayfish hematopoietic tissue (Hpt) cells. The non-toxicity, effectiveness and specificity of histone H2A as a transfection agent provides a cheap, simple, highly efficient and reproducible gene delivery system, particularly for the sensitive cell cultures of crustacean animals such as crayfish and shrimp.

    Nyckelord
    Crayfish, Hematopoietic tissue cells, Histone H2A, RNA interference, Transfection
    Nationell ämneskategori
    Biologiska vetenskaper
    Identifikatorer
    urn:nbn:se:uu:diva-97409 (URN)10.1016/j.dci.2006.07.007 (DOI)000244165900003 ()16999999 (PubMedID)
    Tillgänglig från: 2008-08-18 Skapad: 2008-08-18 Senast uppdaterad: 2017-12-14
    2. Antilipopolysaccharide factor interferes with white spot syndrome virus replication in vitro and in vivo in the crayfish Pacifastacus leniusculus
    Öppna denna publikation i ny flik eller fönster >>Antilipopolysaccharide factor interferes with white spot syndrome virus replication in vitro and in vivo in the crayfish Pacifastacus leniusculus
    Visa övriga...
    2006 (Engelska)Ingår i: Journal of Virology, ISSN 0022-538X, E-ISSN 1098-5514, Vol. 80, nr 21, s. 10365-10371Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    In a study of genes expressed differentially in the freshwater crayfish Pacifastacus leniusculus infected experimentally with the white spot syndrome virus (WSSV), one protein, known as antilipopolysaccharide factor (ALF), was chosen, among those whose transcript levels increased upon viral infection, for further studies. ALF RNA interference (RNAi) experiments in whole animals and in cell cultures indicated that ALF can protect against WSSV infection, since knockdown of AILF by RNAi specifically resulted in higher rates of viral propagation. In a cell culture of hematopoietic tissue (Hpt) from P. leniusculus, quantitative PCR showed that knockdown of ALF by RNAi resulted into WSSV levels that were about 10-fold higher than those treated with control double-stranded RNA (dsRNA). In addition, RNAi experiments with other crayfish genes that had been found to be up-regulated by a WSSV infection did not result in any changes of viral loads. Thus, the cell culture does not respond to dsRNA in a similar manner, as shown earlier for dsRNA injected into shrimp, which gave a higher degree of resistance to WSSV infection. If ALF transcription in whole animals was stimulated by the administration of LTV-treated WSSV, a partial protection against a subsequent challenge with the active virus was conferred to the host. This is the first crustacean gene product identified with the capacity to interfere with replication of this important pathogen.

    Nationell ämneskategori
    Biologiska vetenskaper
    Identifikatorer
    urn:nbn:se:uu:diva-97410 (URN)10.1128/JVI.01101-06 (DOI)000241606100008 ()17041217 (PubMedID)
    Tillgänglig från: 2008-08-18 Skapad: 2008-08-18 Senast uppdaterad: 2017-12-14Bibliografiskt granskad
    3. Phenoloxidase is an important component of the defense against Aeromonas hydrophila infection in a crustacean, Pacifastacus leniusculus
    Öppna denna publikation i ny flik eller fönster >>Phenoloxidase is an important component of the defense against Aeromonas hydrophila infection in a crustacean, Pacifastacus leniusculus
    Visa övriga...
    2007 (Engelska)Ingår i: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 282, nr 46, s. 33593-33598Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    The melanization cascade, in which phenoloxidase is the terminal enzyme, appears to play a key role in recognition of and defense against microbial infections in invertebrates. Here, we show that phenoloxidase activity and melanization are important for the immune defense toward a highly pathogenic bacterium, Aeromonas hydrophila, in the freshwater crayfish, Pacifastacus leniusculus. RNA interference-mediated depletion of crayfish prophenoloxidase leads to increased bacterial growth, lower phagocytosis, lower phenoloxidase activity, lower nodule formation, and higher mortality when infected with this bacterium. In contrast, if RNA interference of pacifastin, an inhibitor of the crayfish prophenoloxidase activation cascade, is performed, it results in lower bacterial growth, increased phagocytosis, increased nodule formation, higher phenoloxidase activity, and delayed mortality. Our data therefore suggest that phenoloxidase is required in crayfish defense against an infection by A. hydrophila, a highly virulent and pathogenic bacterium to crayfish.

    Nationell ämneskategori
    Biologiska vetenskaper
    Identifikatorer
    urn:nbn:se:uu:diva-97411 (URN)10.1074/jbc.M706113200 (DOI)000250840200040 ()17855335 (PubMedID)
    Tillgänglig från: 2008-08-18 Skapad: 2008-08-18 Senast uppdaterad: 2017-12-14Bibliografiskt granskad
    4. Hemocyte lineage marker proteins in a crustacean, the freshwater crayfish, Pacifastacus leniusculus
    Öppna denna publikation i ny flik eller fönster >>Hemocyte lineage marker proteins in a crustacean, the freshwater crayfish, Pacifastacus leniusculus
    Visa övriga...
    2008 (Engelska)Ingår i: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 8, nr 20, s. 4226-4235Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    To identify proteins associated with development of different hemocyte types in the freshwater crayfish Pacifastacus leniusculus, 2-DE followed by MS analysis was carried out with hematopoietic tissue (Hpt) cells, semigranular cells (SGC) and granular cells (GC). Within the hemocyte lineages one two-domain Kazal proteinase inhibitor (KPI) was found to be specific for SGC, while a superoxide dismutase (SOD) was specific for GC at protein as well as at mRNA level. The proliferation cell nuclear antigen (PCNA) was detected at the mRNA level in Hpt cells only. We also provide evidence that SGC and GC most likely differentiate to maturation as separate lineages. We found that after laminarin or lipopolysaccharide (LPS) injection into crayfish, the transcript levels of PCNA and SOD increased in the Hpt cells, whereas the KPI transcript never was present in Hpt regardless of any challenge. RNA interference of PCNA in the Hpt cells led to that most of the cells did not spread or attach to the tissue culture dish. These results suggest that PCNA, KPI and SOD can be used as markers for Hpt cells, SGC and GC, respectively, and in conjunction with these results, a model is proposed how the Hpt responds to a microbial challenge by proliferation and release of Hpt cells.

    Nyckelord
    Crustacean hematopoiesis, Hemocyte markers, Kazal, PCNA, SOD
    Nationell ämneskategori
    Immunologi
    Forskningsämne
    Biologi med inriktning mot jämförande fysiologi; Immunologi
    Identifikatorer
    urn:nbn:se:uu:diva-97412 (URN)10.1002/pmic.200800177 (DOI)000260717300010 ()18814328 (PubMedID)
    Tillgänglig från: 2008-08-18 Skapad: 2008-08-18 Senast uppdaterad: 2017-12-14
  • 152.
    Liu, Haipeng
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Jiravanichpaisal, Pikul
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Cerenius, Lage
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Bok, Luel Lee
    Söderhäll, Irene
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Söderhäll, Kenneth
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Phenoloxidase is an important component of the defense against Aeromonas hydrophila infection in a crustacean, Pacifastacus leniusculus2007Ingår i: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 282, nr 46, s. 33593-33598Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The melanization cascade, in which phenoloxidase is the terminal enzyme, appears to play a key role in recognition of and defense against microbial infections in invertebrates. Here, we show that phenoloxidase activity and melanization are important for the immune defense toward a highly pathogenic bacterium, Aeromonas hydrophila, in the freshwater crayfish, Pacifastacus leniusculus. RNA interference-mediated depletion of crayfish prophenoloxidase leads to increased bacterial growth, lower phagocytosis, lower phenoloxidase activity, lower nodule formation, and higher mortality when infected with this bacterium. In contrast, if RNA interference of pacifastin, an inhibitor of the crayfish prophenoloxidase activation cascade, is performed, it results in lower bacterial growth, increased phagocytosis, increased nodule formation, higher phenoloxidase activity, and delayed mortality. Our data therefore suggest that phenoloxidase is required in crayfish defense against an infection by A. hydrophila, a highly virulent and pathogenic bacterium to crayfish.

  • 153.
    Liu, Haipeng
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Jiravanichpaisal, Pikul
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Söderhäll, Irene
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Cerenius, Lage
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Söderhäll, Kenneth
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Antilipopolysaccharide factor interferes with white spot syndrome virus replication in vitro and in vivo in the crayfish Pacifastacus leniusculus2006Ingår i: Journal of Virology, ISSN 0022-538X, E-ISSN 1098-5514, Vol. 80, nr 21, s. 10365-10371Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    In a study of genes expressed differentially in the freshwater crayfish Pacifastacus leniusculus infected experimentally with the white spot syndrome virus (WSSV), one protein, known as antilipopolysaccharide factor (ALF), was chosen, among those whose transcript levels increased upon viral infection, for further studies. ALF RNA interference (RNAi) experiments in whole animals and in cell cultures indicated that ALF can protect against WSSV infection, since knockdown of AILF by RNAi specifically resulted in higher rates of viral propagation. In a cell culture of hematopoietic tissue (Hpt) from P. leniusculus, quantitative PCR showed that knockdown of ALF by RNAi resulted into WSSV levels that were about 10-fold higher than those treated with control double-stranded RNA (dsRNA). In addition, RNAi experiments with other crayfish genes that had been found to be up-regulated by a WSSV infection did not result in any changes of viral loads. Thus, the cell culture does not respond to dsRNA in a similar manner, as shown earlier for dsRNA injected into shrimp, which gave a higher degree of resistance to WSSV infection. If ALF transcription in whole animals was stimulated by the administration of LTV-treated WSSV, a partial protection against a subsequent challenge with the active virus was conferred to the host. This is the first crustacean gene product identified with the capacity to interfere with replication of this important pathogen.

  • 154.
    Liu, Haipeng
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Söderhäll, Irene
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Histone H2A as a transfection agent in crayfish hematopoietic tissue cells2007Ingår i: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 31, nr 4, s. 340-346Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    We report a novel and highly efficient dsRNA transfection system based on one of the nuclear proteins, namely, histone H2A. RT-PCR semi-quantitative analysis of silencing target gene shows that the transfection efficiency of histone H2A is higher than Effectene or liposome-based transfection systems. Importantly, the high efficiency of histone H2A was associated with very low toxicity to the transfected crayfish hematopoietic tissue (Hpt) cells. The non-toxicity, effectiveness and specificity of histone H2A as a transfection agent provides a cheap, simple, highly efficient and reproducible gene delivery system, particularly for the sensitive cell cultures of crustacean animals such as crayfish and shrimp.

  • 155.
    Liu, Haipeng
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Söderhäll, Kenneth
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Jiravanichpaisal, Pikul
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Antiviral immunity in crustaceans2009Ingår i: Fish and Shellfish Immunology, ISSN 1050-4648, E-ISSN 1095-9947, Vol. 27, nr 2, s. 79-88Artikel, forskningsöversikt (Refereegranskat)
    Abstract [en]

    Viral diseases of shrimp have caused negative effects on the economy in several countries in Asia, south Americas and Americas, where they have numerous shrimp culture industries. The studies on the immunity of shrimp and other crustaceans have mainly focused on general aspects of immunity and as a consequence little is known about the antiviral responses in crustaceans. The aim of this review is to update recent knowledge of innate immunity against viral infections in crustaceans. Several antiviral molecules have been isolated and characterized recently from decapods. Characterization and identification of these molecules might provide a promising strategy for protection and treatment of these viral diseases. In addition dsRNA-induced antiviral immunity is also included.

  • 156.
    Mayo, Susan
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. Jämförande fysiologi.
    Crayfish hematopoiesis; hemocyte development in Pacifastacus leniusculus2002Licentiatavhandling, monografi (Övrigt vetenskapligt)
  • 157.
    Metzendorf, Christoph
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Mitochondrial Iron Metabolism: Study of mitoferrin in Drosophila melanogaster2010Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Iron has a dualistic character. On the one hand it is essential for the life of most organisms, on the other hand it is involved in the generation of reactive oxygen species that are implicated in diseases and aging. During evolution efficient mechanisms for uptake, handling and storage of iron in a safe way have developed to keep the balance between iron availability and minimizing the hazards. In eukaryotes, mitochondria are the central organelle for “metabolizing” iron and consequently play an important role in cellular iron homeostasis.

    Mitoferrins are mitochondrial carrier proteins, which are involved in iron transport into mitochondria. In vertebrates two mitoferrins exist, one (mitoferrin1) of which is essential for heme synthesis during erythropoiesis, while the function of the other (mitoferrin2) is not well defined. In the fruit fly we found only one mitoferrin gene (dmfrn), which codes most likely for a functional homologueof vertebrate mitoferrin2.

    In Drosophila cell culture, dmfrn overexpression resulted in an overestimation of cell sensed iron levels. The signal responsible for this, is most likely a yet unidentified compound of ISC synthesis. In the cell culture system we also showed that iron chelation blocks the progression of the cell cycle in a reversible and therefore most likely controlled way.

    Study of different dmfrn mutants indicates a role of dmfrn during spermatogenesis and development to adulthood. As dmfrn deletion mutants are not lethal, it is likely that other lower affinity iron transporters exist. A similar conclusion has been drawn by others from the study of yeast mitoferrin homologuemutants. Rim2p/Mrs12p has recently been implicated in mitochondrial iron transport, and might be an alternative metal carrier. We identified a putative homologuein the fruit fly and found a possible link between mutants in this gene and iron.

    Our results emphasize the importance of the mitochondrial iron metabolism in cellular iron homeostasis. We also show for the first time, a direct connection between the mitochondrial iron metabolism and spermatogenesis. Mutants characterized and developed by us will help to study these processes in further detail and reveal the underlying mechanisms.

    Delarbeten
    1. Overexpression of Drosophila mitoferrin in l(2)mbn cells results in dysregulation of Fer1HCH expression
    Öppna denna publikation i ny flik eller fönster >>Overexpression of Drosophila mitoferrin in l(2)mbn cells results in dysregulation of Fer1HCH expression
    2009 (Engelska)Ingår i: Biochemical Journal, ISSN 0264-6021, E-ISSN 1470-8728, Vol. 421, nr Part 3, s. 463-471Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Mrs3p and Mrs4p (Mrs3/4p) are yeast mitochondrial iron carrier proteins that play important roles in ISC (iron-sulphur cluster) and haem biosynthesis. At low iron conditions, mitochondrial and cytoplasmic ISC protein maturation is correlated with MRS3/4 expression. Zebrafish mitoferrin1 (mfrn1), one of two MRS3/4 orthologues, is essential for erythropoiesis, but little is known about the ubiquitously expressed paralogue mfrn2. In the present study we identified a single mitoferrin gene (dmfrn) in the genome of Drosophila melanogaster, which is probably an orthologue of mfrn2. Overexpression of dmfrn in the Drosophila l(2)mbn cell line (mbn-dmfrn) resulted in decreased binding between IRP-1A (iron regulatory protein 1A) and stem-loop RNA structures referred to as IREs (iron responsive elements). mbn-dmfrn cell lines also had increased cytoplasmic aconitase activity and slightly decreased iron content. In contrast, iron loading results in decreased IRP-1A-IRE binding, but increased cellular iron content, in experimental mbn-dmfrn and control cell lines. Iron loading also increases cytoplasmic aconitase activity in all cell lines, but with slightly higher activity observed in mbn-dmfrn cells. From this we concluded that dmfrn overexpression stimulates cytoplasmic ISC protein maturation, as has been reported for MRS3/4 overexpression. Compared with control cell lines, mbn-dmfrn cells had higher Fer1HCH (ferritin 1 heavy chain homologue) transcript and protein levels. RNA interference of the putative Drosophila orthologue of human ABCB7, a mitochondrial transporter involved in cytoplasmic ISC protein maturation, restored Fer1HCH transcript levels of iron-treated mbn-dmfrn cells to those of control cells grown in normal medium. These results suggest that dmfrn overexpression in l(2)mbn cells causes an 'overestimation' of the cellular iron content, and that regulation of Fer1HCH transcript abundance probably depends on cytoplasmic ISC protein maturation.

    Nyckelord
    Drosophila, iron homoeostasis, iron transport, mitochondrial iron metabolism
    Nationell ämneskategori
    Biologiska vetenskaper
    Forskningsämne
    Biologi med inriktning mot jämförande fysiologi
    Identifikatorer
    urn:nbn:se:uu:diva-114111 (URN)10.1042/BJ20082231 (DOI)000268615900014 ()19453295 (PubMedID)
    Tillgänglig från: 2010-02-10 Skapad: 2010-02-10 Senast uppdaterad: 2017-12-12Bibliografiskt granskad
    2. Drosophila mitoferrin is essential for male fertility: Evidence for a role of mitochondrial iron metabolism during spermatogenesis
    Öppna denna publikation i ny flik eller fönster >>Drosophila mitoferrin is essential for male fertility: Evidence for a role of mitochondrial iron metabolism during spermatogenesis
    2010 (Engelska)Ingår i: BMC Developmental Biology, ISSN 1471-213X, E-ISSN 1471-213X, Vol. 10, s. 68-Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Background: Mammals and Drosophila melanogaster share some striking similarities in spermatogenesis. Mitochondria in spermatids undergo dramatic morphological changes and syncytial spermatids are stripped from their cytoplasm and then individually wrapped by single membranes in an individualization process. In mammalian and fruit fly testis, components of the mitochondrial iron metabolism are expressed, but so far their function during spermatogenesis is unknown. Here we investigate the role of Drosophila mitoferrin (dmfrn), which is a mitochondrial carrier protein with an established role in the mitochondrial iron metabolism, during spermatogenesis.

    Results: We found that P-element insertions into the 5'-untranslated region of the dmfrn gene cause recessive male sterility, which was rescued by a fluorescently tagged transgenic dmfrn genomic construct (dmfrn(venus)). Testes of mutant homozygous dmfrn(SH115) flies were either small with unorganized content or contained some partially elongated spermatids, or testes were of normal size but lacked mature sperm. Testis squashes indicated that spermatid elongation was defective and electron micrographs showed mitochondrial defects in elongated spermatids and indicated failed individualization. Using a LacZ reporter and the dmfrn(venus) transgene, we found that dmfrn expression in testes was highest in spermatids, coinciding with the stages that showed defects in the mutants. Dmfrn-venus protein accumulated in mitochondrial derivatives of spermatids, where it remained until most of it was stripped off during individualization and disposed of in waste bags. Male sterility in flies with the hypomorph alleles dmfrn(BG00456) and dmfrn(EY01302) over the deletion Df(3R)ED6277 was increased by dietary iron chelation and suppressed by iron supplementation of the food, while male sterility of dmfrn(SH115)/Df(3R)ED6277 flies was not affected by food iron levels.

    Conclusions: In this work, we show that mutations in the Drosophila mitoferrin gene result in male sterility caused by developmental defects. From the sensitivity of the hypomorph mutants to low food iron levels we conclude that mitochondrial iron is essential for spermatogenesis. This is the first time that a link between the mitochondrial iron metabolism and spermatogenesis has been shown. Furthermore, due to the similar expression patterns of some mitochondrial iron metabolism genes in Drosophila and mammals, it is likely that our results are applicable for mammals as well.

    Nyckelord
    mitoferrin, Drosophila, mitochondria, spermatogenesis, iron
    Nationell ämneskategori
    Biologiska vetenskaper
    Forskningsämne
    Biologi med inriktning mot jämförande fysiologi
    Identifikatorer
    urn:nbn:se:uu:diva-114198 (URN)10.1186/1471-213X-10-68 (DOI)000280367400001 ()20565922 (PubMedID)
    Anmärkning
    Uppdaterad från Manuskript till Artikel 20101208Tillgänglig från: 2010-02-11 Skapad: 2010-02-11 Senast uppdaterad: 2017-12-12Bibliografiskt granskad
    3. Characterization of Drosophila mitoferrin and CG18317 mutants
    Öppna denna publikation i ny flik eller fönster >>Characterization of Drosophila mitoferrin and CG18317 mutants
    (Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
    Abstract [en]

    Introduction: Mitochondria are the site of iron-sulfur cluster synthesis and insertion of iron into heme, two essential prosthetic groups. Mrs3/4/mitoferrins are mitochondrial carrier proteins, which are involved in the mitochondrial iron transport. Yeast mutants suggest that a low-affinity iron transport system exists. Previous analysis of Drosophila mitoferrin P element mutants revealed an involvement of dmfrn in spermatogenesis. The ubiquitous expression of dmfrn in several adult male tissues, did however suggest a more general role.

    Results: Here we analyzed stocks with deletions in dmfrn and find that on low iron food dmfrn deletion inhibits development to adulthood, whereas on normal and high iron food some escapers do eclose. RNAi of dmfrn driven by the ubiquitous Actin5C-Gal4 driver recapitulates the phenotype on low iron food, but not on normal or high iron food. Overexpression of dmfrn from an UAS-dmfrn transgenic construct that contains only the coding region of dmfrn also resulted in the failure to develop to adulthood on low iron food. We identified CG18317 as putative Drosophila homolog of yeast Rim2p/Mrs12p, a mitochondrial pyrimidine carrier that is implicated in low affinity iron transport. CG18317 is ubiquitously expressed in several tissues of the male fly and the second exon is alternatively spliced resulting in a 21 nucleotides shorter transcript. An initial study of transposon mutants and deletion mutants of this gene showed sensitivity of one transposon mutant to low iron food conditions. Preliminary results indicate that increased expression of CG18317 might rescue development of dmfrn deletion mutants to at least pupal stage.

    Conclusions: In contrast to yeast MRS3/4, Drosophila mitoferrin is an essential gene, necessary for development of Drosophila to adulthood. The presence of escapers does however suggest, that dmfrn is either not the only mitochondrial iron carrier or that dmfrn modulates activity of a mitochondrial iron transport system. The sensitivity to low iron conditions of one CG18317 mutant and the partial rescue of dmfrn mutants indicates that this gene could indeed mediate mitochondrial iron uptake in the fly.

    Nyckelord
    mitoferrin, Drosophila, iron, mitochondria, MRS12
    Nationell ämneskategori
    Biologiska vetenskaper
    Forskningsämne
    Biologi med inriktning mot jämförande fysiologi
    Identifikatorer
    urn:nbn:se:uu:diva-114192 (URN)
    Tillgänglig från: 2010-02-11 Skapad: 2010-02-11 Senast uppdaterad: 2010-02-11
    4. The role of iron in the proliferation of Drosophila l(2)mbn cells
    Öppna denna publikation i ny flik eller fönster >>The role of iron in the proliferation of Drosophila l(2)mbn cells
    2010 (Engelska)Ingår i: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 400, nr 3, s. 442-446Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Iron is essential for life and is needed for cell proliferation and cell cycle progression. Iron deprivation induces cell cycle arrest and finally apoptosis. The Drosophila tumorous larval hemocyte cell line l(2)mbn was used to study the sensitivity and cellular response to iron deprivation through the chelator desferrioxamine (DFO). At a concentration of 10 mM DFO or more the proliferation was inhibited reversibly, while the amount of dead cells did not increase. FACS analysis showed that the cell cycle was arrested in G1/S phase and the transcript level of cycE was decreased to less than 50% of control cells. These results show that iron chelation in this insect tumorous cell line causes a specific and coordinated cell cycle arrest.

    Nyckelord
    iron drosophila cell cycle arrest cyclin cell proliferation
    Nationell ämneskategori
    Biokemi och molekylärbiologi
    Forskningsämne
    Biologi med inriktning mot jämförande fysiologi
    Identifikatorer
    urn:nbn:se:uu:diva-114118 (URN)10.1016/j.bbrc.2010.08.100 (DOI)000282616200027 ()
    Tillgänglig från: 2010-02-10 Skapad: 2010-02-10 Senast uppdaterad: 2017-12-12Bibliografiskt granskad
  • 158.
    Metzendorf, Christoph
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    The Drosophila mitoferrin gene-identification and characterisation2009Licentiatavhandling, sammanläggning (Övrigt vetenskapligt)
  • 159.
    Metzendorf, Christoph
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Lind, Maria I.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Drosophila mitoferrin is essential for male fertility: Evidence for a role of mitochondrial iron metabolism during spermatogenesis2010Ingår i: BMC Developmental Biology, ISSN 1471-213X, E-ISSN 1471-213X, Vol. 10, s. 68-Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: Mammals and Drosophila melanogaster share some striking similarities in spermatogenesis. Mitochondria in spermatids undergo dramatic morphological changes and syncytial spermatids are stripped from their cytoplasm and then individually wrapped by single membranes in an individualization process. In mammalian and fruit fly testis, components of the mitochondrial iron metabolism are expressed, but so far their function during spermatogenesis is unknown. Here we investigate the role of Drosophila mitoferrin (dmfrn), which is a mitochondrial carrier protein with an established role in the mitochondrial iron metabolism, during spermatogenesis.

    Results: We found that P-element insertions into the 5'-untranslated region of the dmfrn gene cause recessive male sterility, which was rescued by a fluorescently tagged transgenic dmfrn genomic construct (dmfrn(venus)). Testes of mutant homozygous dmfrn(SH115) flies were either small with unorganized content or contained some partially elongated spermatids, or testes were of normal size but lacked mature sperm. Testis squashes indicated that spermatid elongation was defective and electron micrographs showed mitochondrial defects in elongated spermatids and indicated failed individualization. Using a LacZ reporter and the dmfrn(venus) transgene, we found that dmfrn expression in testes was highest in spermatids, coinciding with the stages that showed defects in the mutants. Dmfrn-venus protein accumulated in mitochondrial derivatives of spermatids, where it remained until most of it was stripped off during individualization and disposed of in waste bags. Male sterility in flies with the hypomorph alleles dmfrn(BG00456) and dmfrn(EY01302) over the deletion Df(3R)ED6277 was increased by dietary iron chelation and suppressed by iron supplementation of the food, while male sterility of dmfrn(SH115)/Df(3R)ED6277 flies was not affected by food iron levels.

    Conclusions: In this work, we show that mutations in the Drosophila mitoferrin gene result in male sterility caused by developmental defects. From the sensitivity of the hypomorph mutants to low food iron levels we conclude that mitochondrial iron is essential for spermatogenesis. This is the first time that a link between the mitochondrial iron metabolism and spermatogenesis has been shown. Furthermore, due to the similar expression patterns of some mitochondrial iron metabolism genes in Drosophila and mammals, it is likely that our results are applicable for mammals as well.

  • 160.
    Metzendorf, Christoph
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Lind, Maria I
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    The role of iron in the proliferation of Drosophila l(2)mbn cells2010Ingår i: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 400, nr 3, s. 442-446Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Iron is essential for life and is needed for cell proliferation and cell cycle progression. Iron deprivation induces cell cycle arrest and finally apoptosis. The Drosophila tumorous larval hemocyte cell line l(2)mbn was used to study the sensitivity and cellular response to iron deprivation through the chelator desferrioxamine (DFO). At a concentration of 10 mM DFO or more the proliferation was inhibited reversibly, while the amount of dead cells did not increase. FACS analysis showed that the cell cycle was arrested in G1/S phase and the transcript level of cycE was decreased to less than 50% of control cells. These results show that iron chelation in this insect tumorous cell line causes a specific and coordinated cell cycle arrest.

  • 161.
    Metzendorf, Christoph
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Wiesenberger, Gerlinde
    Department of Applied Genetics and Cell Biology University of Natural Resources and Applied Life Sciences (BOKU) Muthgasse 18 1190 Vienna Austria.
    Lind, Maria I.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Characterization of Drosophila mitoferrin and CG18317 mutantsManuskript (preprint) (Övrigt vetenskapligt)
    Abstract [en]

    Introduction: Mitochondria are the site of iron-sulfur cluster synthesis and insertion of iron into heme, two essential prosthetic groups. Mrs3/4/mitoferrins are mitochondrial carrier proteins, which are involved in the mitochondrial iron transport. Yeast mutants suggest that a low-affinity iron transport system exists. Previous analysis of Drosophila mitoferrin P element mutants revealed an involvement of dmfrn in spermatogenesis. The ubiquitous expression of dmfrn in several adult male tissues, did however suggest a more general role.

    Results: Here we analyzed stocks with deletions in dmfrn and find that on low iron food dmfrn deletion inhibits development to adulthood, whereas on normal and high iron food some escapers do eclose. RNAi of dmfrn driven by the ubiquitous Actin5C-Gal4 driver recapitulates the phenotype on low iron food, but not on normal or high iron food. Overexpression of dmfrn from an UAS-dmfrn transgenic construct that contains only the coding region of dmfrn also resulted in the failure to develop to adulthood on low iron food. We identified CG18317 as putative Drosophila homolog of yeast Rim2p/Mrs12p, a mitochondrial pyrimidine carrier that is implicated in low affinity iron transport. CG18317 is ubiquitously expressed in several tissues of the male fly and the second exon is alternatively spliced resulting in a 21 nucleotides shorter transcript. An initial study of transposon mutants and deletion mutants of this gene showed sensitivity of one transposon mutant to low iron food conditions. Preliminary results indicate that increased expression of CG18317 might rescue development of dmfrn deletion mutants to at least pupal stage.

    Conclusions: In contrast to yeast MRS3/4, Drosophila mitoferrin is an essential gene, necessary for development of Drosophila to adulthood. The presence of escapers does however suggest, that dmfrn is either not the only mitochondrial iron carrier or that dmfrn modulates activity of a mitochondrial iron transport system. The sensitivity to low iron conditions of one CG18317 mutant and the partial rescue of dmfrn mutants indicates that this gene could indeed mediate mitochondrial iron uptake in the fly.

  • 162.
    Metzendorf, Christoph
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Wu, Wenlin
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Overexpression of dmrs, a Drosophila homologue of yeast mrs3/4, results in increased expression of ferritin heavy chain homologue2007Konferensbidrag (Refereegranskat)
  • 163.
    Metzendorf, Christoph
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Wu, Wenlin
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Lind, Maria I
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Overexpression of dmrs, a Drosophila mitochondrial transporter, results in disruption of normal iron homeostasis in the Drosophila cell line mbn(2).2007Konferensbidrag (Refereegranskat)
  • 164.
    Metzendorf, Christoph
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Wu, Wenlin
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Lind, Maria I
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Overexpression of dmrs, a Drosophila mitochondrial transporter, results in disruption of normal iron homeostasis in the Drosophila cell line mbn(2).2007Konferensbidrag (Refereegranskat)
  • 165.
    Metzendorf, Christoph
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Wu, Wenlin
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Lind, Maria I
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Overexpression of Drosophila mitoferrin in l(2)mbn cells results in dysregulation of Fer1HCH expression2009Ingår i: Biochemical Journal, ISSN 0264-6021, E-ISSN 1470-8728, Vol. 421, nr Part 3, s. 463-471Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Mrs3p and Mrs4p (Mrs3/4p) are yeast mitochondrial iron carrier proteins that play important roles in ISC (iron-sulphur cluster) and haem biosynthesis. At low iron conditions, mitochondrial and cytoplasmic ISC protein maturation is correlated with MRS3/4 expression. Zebrafish mitoferrin1 (mfrn1), one of two MRS3/4 orthologues, is essential for erythropoiesis, but little is known about the ubiquitously expressed paralogue mfrn2. In the present study we identified a single mitoferrin gene (dmfrn) in the genome of Drosophila melanogaster, which is probably an orthologue of mfrn2. Overexpression of dmfrn in the Drosophila l(2)mbn cell line (mbn-dmfrn) resulted in decreased binding between IRP-1A (iron regulatory protein 1A) and stem-loop RNA structures referred to as IREs (iron responsive elements). mbn-dmfrn cell lines also had increased cytoplasmic aconitase activity and slightly decreased iron content. In contrast, iron loading results in decreased IRP-1A-IRE binding, but increased cellular iron content, in experimental mbn-dmfrn and control cell lines. Iron loading also increases cytoplasmic aconitase activity in all cell lines, but with slightly higher activity observed in mbn-dmfrn cells. From this we concluded that dmfrn overexpression stimulates cytoplasmic ISC protein maturation, as has been reported for MRS3/4 overexpression. Compared with control cell lines, mbn-dmfrn cells had higher Fer1HCH (ferritin 1 heavy chain homologue) transcript and protein levels. RNA interference of the putative Drosophila orthologue of human ABCB7, a mitochondrial transporter involved in cytoplasmic ISC protein maturation, restored Fer1HCH transcript levels of iron-treated mbn-dmfrn cells to those of control cells grown in normal medium. These results suggest that dmfrn overexpression in l(2)mbn cells causes an 'overestimation' of the cellular iron content, and that regulation of Fer1HCH transcript abundance probably depends on cytoplasmic ISC protein maturation.

  • 166. Muñoz-Alarcón, Andrés
    et al.
    Pavlovic, Maja
    Wismar, Jasmine
    Schmitt, Bertram
    Eriksson, Maria
    Kylsten, Per
    Dushay, Mitchell S.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Characterization of lamin mutation phenotypes in Drosophila and comparison to human laminopathies2007Ingår i: PLoS ONE, ISSN 1932-6203, Vol. 2, nr 6, s. e532-Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Lamins are intermediate filament proteins that make up the nuclear lamina, a matrix underlying the nuclear membrane in all metazoan cells that is important for nuclear form and function. Vertebrate A-type lamins are expressed in differentiating cells, while B-type lamins are expressed ubiquitously. Drosophila has two lamin genes that are expressed in A- and B-type patterns, and it is assumed that similarly expressed lamins perform similar functions. However, Drosophila and vertebrate lamins are not orthologous, and their expression patterns evolved independently. It is therefore of interest to examine the effects of mutations in lamin genes. Mutations in the mammalian lamin A/C gene cause a range of diseases, collectively called laminopathies, that include muscular dystrophies and premature aging disorders. We compared the sequences of lamin genes from different species, and we have characterized larval and adult phenotypes in Drosophila bearing mutations in the lam gene that is expressed in the B-type pattern. Larvae move less and show subtle muscle defects, and surviving lam adults are flightless and walk like aged wild-type flies, suggesting that lam phenotypes might result from neuromuscular defects, premature aging, or both. The resemblance of Drosophila lam phenotypes to human laminopathies suggests that some lamin functions may be performed by differently expressed genes in flies and mammals. Such still-unknown functions thus would not be dependent on lamin gene expression pattern, suggesting the presence of other lamin functions that are expression dependent. Our results illustrate a complex interplay between lamin gene expression and function through evolution.

  • 167. Oidtmann, B
    et al.
    Cerenius, Lage
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Schmid, I
    Hoffmann, R
    Söderhäll, Kenneth
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Crayfish plague epizootics in Germany - classification of two German isolates of the crayfish plague fungus Aphanomyces astaci by random amplification of polymorphic DNA1999Ingår i: Diseases of Aquatic Organisms, ISSN 0177-5103, E-ISSN 1616-1580, Vol. 35, nr 3, s. 235-238Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Following 2 outbreaks of crayfish plague in southern Germany, the causative agent, the oomycete fungus Aphanomyces astaci, was isolated from the diseased Astacus astacus. The identity of the 2 strains was confirmed using established techniques, such as ph

  • 168. Oidtmann, B
    et al.
    Schaefers, N
    Cerenius, Lage
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Söderhäll, Kenneth
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Hoffmann, R W
    Detection of genomic DNA of the crayfish plague fungus Aphanomyces astaci (Oomycete) in clinical samples by PCR.2004Ingår i: Veterinary Microbiology, ISSN 0378-1135, E-ISSN 1873-2542, Vol. 100, nr 3-4, s. 269-82Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A diagnostic procedure, based on a polymerase chain reaction method (PCR) was developed to detect infection of crayfish with the Oomycete Aphanomyces astaci. A set of oligonucleotide primers was designed to specifically amplify A. astaci DNA in the ITS region surrounding the 5.8S rDNA gene. The PCR amplifies a 115 bp amplicon. The specificity of the primers was demonstrated by testing on 27 A. astaci strains and against 20 non-A. astaci Oomycetes and 5 fungal species. Most of the non-A. astaci Oomycete or fungal species included in the study are either known parasites of freshwater crayfish cuticle or can be found in their natural environment. Specificity was also tested against crayfish tissue and some known parasites and bacteria infecting crayfish.A protocol for the extraction of A. astaci DNA from infected crayfish tissue was developed. The optimised method allows the detection of two genome equivalents of purified A. astaci genomic DNA.The method was tested on noble crayfish (Astacus astacus), artificially infected with A. astaci. Detection of A. astaci was possible at the very first time of sampling, which was 2 days after the beginning of spore exposure

  • 169.
    Overli, O
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för evolutionsbiologi. Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Harris, CA
    Winberg, S
    Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. Jämförande fysiologi.
    Short-term effects of fights for social dominance and the establishment of dominant-subordinate relationships on brain monoamines and cortisol in rainbow trout1999Ingår i: BRAIN BEHAVIOR AND EVOLUTION, ISSN 0006-8977, Vol. 54, nr 5, s. 263-275Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    We report changes in brain serotonergic, noradrenergic and dopaminergic activity, along with plasma cortisol concentrations, occurring during the initial 24-h period following the establishment of dominant-subordinate relationships in pairs of rainbow tro

  • 170.
    Overli, O
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för evolutionsbiologi. Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Pall, M
    Borg, B
    Jobling, M
    Winberg, S
    Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. jämförande fysiologi.
    Effects of Schistocephalus solidus infection on brain monoaminergic activity in female three-spined sticklebacks Gasterosteus aculeatus2001Ingår i: PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, ISSN 0962-8452, Vol. 268, nr 1474, s. 1411-1415Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The three-spined stickleback Gasterosteus aculeatus is an intermediate host of the tapeworm Schistocephalus solidus. Changes in predator avoidance, foraging and shoaling behaviour have been reported in sticklebacks infested with S. solidus, but the mechan

  • 171. Oweson, Carolina
    et al.
    Li, Chenghua
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Söderhäll, Irene
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för organismbiologi.
    Hernroth, Bodil
    Effects of manganese and hypoxia on coelomocyte renewal in the echinoderm, Asterias rubens (L.)2010Ingår i: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 100, nr 1, s. 84-90Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Manganese (Mn) is a naturally abundant metal and particularly so in soft-bottom oceanic sediments where it generally occurs bound in a four-valent colloidal state as MnO2. When hypoxic conditions occur in bottom waters, the metal reduces to the bioavailable ion Mn2+ and can reach concentrations known to have immunotoxic effects in the crustacean Nephrops norvegicus, reducing numbers of circulating haemocytes as a consequence. However, we have previously shown that Mn seems to have a contrasting effect on the echinoderm Asterias rubens in which it triggers the proliferation of haematopoietic cells and increases coelomocyte numbers. Since elevated Mn levels mostly co-occur with hypoxia in nature, here we investigated whether hypoxia has a negative effect on haematopoiesis. Proliferation and differentiation of coelomocytes and cells in the coelomic epithelium of A. rubens were compared after 3 days of exposure to realistic levels of Mn, hypoxia or a combination of these two parameters. We can confirm that Mn elevated numbers of coelomocytes and increased proliferation of epithelial cells, but hypoxia did not affect these levels. However, hypoxia did affect differentiation of these cells as judged by investigating the expression of a Runt domain transcription factor, which was also cloned and sequenced. Through comparative quantification using a real time PCR technique, we found that exposure to hypoxia had a clearly stimulating effect on mRNA expression of Runt gene in both coelomocytes and epithelial cells. These results indicate that during hypoxic conditions the composition of coelomocyte sub-populations changed.

  • 172. Park, Ji Won
    et al.
    Je, Byung-Rok
    Piao, Shunfu
    Inamura, Seiichi
    Fujimoto, Yukari
    Fukase, Koichi
    Kusumoto, Shoichi
    Soderhall, Kenneth
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Ha, Nam-Chul
    Lee, Bok Luel
    A synthetic peptidoglycan fragment as a competitive inhibitor of the melanization cascade2006Ingår i: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 281, nr 12, s. 7747-7755Artikel i tidskrift (Refereegranskat)
  • 173. Ragan, MA
    et al.
    Goggin, CL
    Cawthorn, RJ
    Cerenius, Lage
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Jamieson, AVC
    Plourde, SM
    Rand, TG
    Söderhäll, Kenneth
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Gutell, RR
    A novel clade of protistan parasites near the animal-fungal divergence1996Ingår i: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 93, nr 21, s. 11907-11912Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Sequences of nuclear-encoded small-subunit rRNA genes have been determined for representatives of the enigmatic genera Dermocystidium, Ichthyophonus, and Psorospermium, protistan parasites of fish and crustaceans. The small-subunit rRNA genes from these p

  • 174. Roh, Kyung-Baeg
    et al.
    Kim, Chan-Hee
    Lee, Hanna
    Kwon, Hyun-Mi
    Park, Ji-Won
    Ryu, Ji-Hwan
    Kurokawa, Kenji
    Ha, Nam-Chul
    Lee, Won-Jae
    Lemaitre, Bruno
    Söderhäll, Kenneth
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Lee, Bok-Luel
    Proteolytic cascade for the activation of the insect toll pathway induced by the fungal cell wall component2009Ingår i: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 284, nr 29, s. 19474-19481Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The insect Toll signaling pathway is activated upon recognition of Gram-positive bacteria and fungi, resulting in the expression of antimicrobial peptides via NF-kappaB-like transcription factor. This activation is mediated by a serine protease cascade leading to the processing of Spätzle, which generates the functional ligand of the Toll receptor. Recently, we identified three serine proteases mediating Toll pathway activation induced by lysine-type peptidoglycan of Gram-positive bacteria. However, the identities of the downstream serine protease components of Gram-negative-binding protein 3 (GNBP3), a receptor for a major cell wall component beta-1,3-glucan of fungi, and their order of activation have not been characterized yet. Here, we identified three serine proteases that are required for Toll activation by beta-1,3-glucan in the larvae of a large beetle, Tenebrio molitor. The first one is a modular serine protease functioning immediately downstream of GNBP3 that proteolytically activates the second one, a Spätzle-processing enzyme-activating enzyme that in turn activates the third serine protease, a Spätzle-processing enzyme. The active form of Spätzle-processing enzyme then cleaves Spätzle into the processed Spätzle as Toll ligand. In addition, we show that injection of beta-1,3-glucan into Tenebrio larvae induces production of two antimicrobial peptides, Tenecin 1 and Tenecin 2, which are also inducible by injection of the active form of Spätzle-processing enzyme-activating enzyme or processed Spätzle. These results demonstrate a three-step proteolytic cascade essential for the Toll pathway activation by fungal beta-1,3-glucan in Tenebrio larvae, which is shared with lysine-type peptidoglycan-induced Toll pathway activation.

  • 175. Romo-Figueroa, Mari­a Gabriela
    et al.
    Vargas-Requena, Claudia
    Sotelo-Mundo, Rogerio R
    Vargas-Albores, Francisco
    Higuera-Ciapara, Inocencio
    Söderhäll, Kenneth
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. jämförande fysiologi.
    Yepiz-Plascencia, Gloria
    Molecular cloning of a beta-glucan pattern-recognition lipoprotein from the white shrimp Penaeus (Litopenaeus) vannamei: correlations between the deduced amino acid sequence and the native protein structure.2004Ingår i: Dev Comp Immunol, ISSN 0145-305X, Vol. 28, nr 7-8, s. 713-26Artikel i tidskrift (Refereegranskat)
  • 176.
    Royo, Felix
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. Jämförande fysiologi.
    Andersson, Gunnar
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. Jämförande fysiologi.
    Bangyeekhun, Eakaphun
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. Jämförande fysiologi.
    Mazquiz, Jose Luis
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. jämförande fysiologi.
    Söderhäll, Kenneth
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. jämförande fysiologi.
    Cerenius, Lage
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. jämförande fysiologi.
    Physiological and genetic characterisation of some new Aphanomyces strains isolated from freshwater crayfish.2004Ingår i: Vet Microbiol, ISSN 0378-1135, Vol. 104, nr 1-2, s. 103-12Artikel i tidskrift (Refereegranskat)
  • 177. Ruiz-Gomez, Maria de Lourdes
    et al.
    Kittilsen, Silje
    Höglund, Erik
    Huntingford, Felicity A
    Sørensen, Christina
    Pottinger, Thomas G
    Bakken, Morten
    Winberg, Svante
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Korzan, Wayne J
    Overli, Oyvind
    Behavioral plasticity in rainbow trout (Oncorhynchus mykiss) with divergent coping styles: when doves become hawks2008Ingår i: Hormones and Behavior, ISSN 0018-506X, E-ISSN 1095-6867, Vol. 54, nr 4, s. 534-8Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Consistent and heritable individual differences in reaction to challenges, often referred to as stress coping styles, have been extensively documented in vertebrates. In fish, selection for divergent post-stress plasma cortisol levels in rainbow trout (Oncorhynchus mykiss) has yielded a low (LR) and a high responsive (HR) strain. A suite of behavioural traits is associated with this physiological difference, with LR (proactive) fish feeding more rapidly after transfer to a new environment and being socially dominant over HR (reactive) fish. Following transport from the UK to Norway, a switch in behavioural profile occurred in trout from the 3rd generation; HR fish regained feeding sooner than LR fish in a novel environment and became dominant in size-matched HR-LR pairs. One year after transport, HR fish still fed sooner, but no difference in social dominance was found. Among offspring of transported fish, no differences in feeding were observed, but as in pre-transported 3rd generation fish, HR fish lost fights for social dominance against size-matched LR opponents. Transported fish and their offspring retained their distinctive physiological profile throughout the study; HR fish showed consistently higher post-stress cortisol levels at all sampling points. Altered risk-taking and social dominance immediately after transport may be explained by the fact that HR fish lost more body mass during transport than did LR fish. These data demonstrate that some behavioural components of stress coping styles can be modified by experience, whereas behavioural plasticity is limited by genetic effects determining social position early in life story.

  • 178. Scherfer, C.
    et al.
    Qazi, M.R.
    Takahaski, K.
    Ueda, R
    Dushay, M.S.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. jämförande fysiologi.
    The Toll immune-regulated Drosophila protein Fondue is involved in hemolymph clotting and puparium formation2006Ingår i: Developmental Biology, Vol. 295, s. 156-163Artikel i tidskrift (Refereegranskat)
  • 179.
    Schjolden, Joachim
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Backström, Tobias
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Pulman, Kim G.T.
    Pottinger, Tom G.
    Winberg, Svante
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Divergence in behavioural responses to stress in two strains of rainbow trout (Oncorhynchus mykiss) with contrasting stress responsiveness2005Ingår i: Hormones and Behavior, ISSN 0018-506X, E-ISSN 1095-6867, Vol. 48, nr 5, s. 537-544Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The aim of this study was to establish whether two lines of rainbow trout divergent for their plasma cortisol response to a standardized stressor would show consistent differences in their behavioural response to a range of challenging situations. Our results show that the high- and low-responding (HR and LR) lines of rainbow trout did not differ in the aggression shown towards an intruder or in their response to the introduction of a novel object to their home environment. However, there was a difference in behaviour between the two selection lines when they were exposed to two unfamiliar environments. These results suggest that the behaviour of the HR and LR fish differs when they are challenged in unfamiliar environments, while their behaviour does not differ when they are challenged in their home environment. These observations are in agreement with studies on mammals that show that individuals with reactive coping styles perform similarly to proactive animals when they are challenged in a familiar environment, while they show different behaviour when they are challenged in unfamiliar environments. Thus, these results provide further evidence that the HR and LR selection lines of rainbow trout exemplify the two different coping styles described in mammals.

  • 180. Schjolden, Joachim
    et al.
    Backström, Tobias
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Pulman, Kim
    Pottinger, Thomas
    Winberg, Svante
    Divergence in behavioural responses to stress in two strains of rainbow trout (Onchorhyncus mykiss) with contrasting stress responsiveness2005Ingår i: Hormones and Behavior, Vol. 48, nr 5, s. 537-544Artikel i tidskrift (Refereegranskat)
  • 181.
    Schjolden, Joachim
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Pulman, Kim
    Metcalfe, Niel
    Winberg, Svante
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Divergence in locomotor activity between two strains of rainbow trout Oncorhynchus mykiss with contrasting stress responsiveness2006Ingår i: Journal of Fish Biology, ISSN 0022-1112, E-ISSN 1095-8649, Vol. 68, nr 3, s. 920-924Artikel i tidskrift (Övrig (populärvetenskap, debatt, mm))
    Abstract [en]

    Individuals of rainbow trout Oncorhynchus mykiss with a low cortisol response to stress showed higer locomotor activity compared to individuals with a high cortisol response.

  • 182.
    Schjolden, Joachim
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. Jämförande Fysiologi.
    Pulman, Kim
    Pottinger, Tom
    Tottmar, Olof
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. Jämförande Fysiologi.
    Winberg, Svante
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. Jämförande Fysiologi.
    Serotonergic characteristics of rainbow trout divergent in stress responsiveness2006Ingår i: PHYSIOLOGY & BEHAVIOR, ISSN 0031-9384, Vol. 87, nr 5, s. 938-947Artikel i tidskrift (Övrig (populärvetenskap, debatt, mm))
    Abstract [en]

    Juvenile rainbow trout divergent in their cortisol response to confinement stress (HR: high responsive or LR: low responsive fish) were exposed to either I or 3 h of confinement stress. Untreated fish served as control. After the exposure blood and brain samples were collected. From the blood samples, the levels of cortisol and catecholamines were determined, while the brain serotonergic and monoamineoxidase (MAO) activity was determined in four different brain areas (brain stem, hypothalamus, telencephalon and optic rectum). Our results show that the LR fish responds to handling stress with a higher increase in plasma epinephrine compared to HR fish. Our results also show that confinement stress leads to a larger increase in the serotonergic activity in the brain stem and telencephalon in LR fish compared to HR fish. These results support the hypothesis that stress coping strategies similar to those described in mammals also exists in fish. Further, our results have shown that the MAO activity increases in optic tectum and hypothalamus of rainbow trout during confinement stress, while it remains unchanged or decreases in brain stem and telencephalon. Moreover, the MAO activity does not differ between the two selection lines. This indicates that MAO participates actively in the stress response without contributing to the differences in stress coping strategies. (c) 2006 Elsevier Inc. All rights reserved.

  • 183.
    Schjolden, Joachim
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Winberg, Svante
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Genetically determined variation in stress responsiveness in rainbow trout: behavior and neurobiology2007Ingår i: Brain, behavior, and evolution, ISSN 0006-8977, E-ISSN 1421-9743, Vol. 70, nr 4, s. 227-238Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    It is becoming increasingly recognized that the diversity in stressors, their intensity, predictability and the context in which they are experienced, will result in behavioral and physiological responses just as diverse. In addition, stress responses are characterized by individual variations where the physiological and behavioral reactions are associated in such a manner that distinct stress coping styles encompassing suites of correlated traits can be identified. These are often referred to as proactive and reactive stress coping styles. Proactive coping is characterized by more aggression, higher general activity and higher sympathetic activation, whereas reactive coping is characterized by immobility, lack of initiative and a higher parasympathetic/hypothalamic activation. Stable coping styles appear to coexist within populations, and these strategies appear to be largely innate. Moreover, the physiological and behavioral traits of coping styles appear to be heritable. These stress coping styles have proven to play a major role in competitive ability and subsequent social position in different species of vertebrates. However, there are also studies showing that social position can affect parameters encompassing the stress coping style of individuals. In this regard it is important, but not always easy, to distinguish between causes and effects of behavioral and physiological responses to stressors. The question raised is to what extent and rigidness stress coping styles are guided by genetic factors.

  • 184. Schmidt, Otto
    et al.
    Söderhäll, Kenneth
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Theopold, Ulrich
    Stockholm University.
    Faye, Ingrid
    Stockholm University.
    Role of adhesion in arthropod immune recognition2010Ingår i: Annual Review of Entomology, ISSN 0066-4170, E-ISSN 1545-4487, Vol. 55, s. 485-504Artikel, forskningsöversikt (Refereegranskat)
    Abstract [en]

    The recognition and inactivation of toxins and pathogens are mediated by a combination of cell-free and cellular mechanisms. A number of soluble and membrane-bound pattern recognition molecules interact with elicitors to become involved in both cell-free inactivation as well as cellular uptake reactions. Here we describe the possible recognition and effector function of key arthropod immune proteins, such as peroxinectin, hemolin, and hemomucin, as an outcome of changes in adhesiveness, which drive self-assembly reactions leading to cell-free coagulation and cellular uptake reactions. The fact that some of these proteins are essential for immune and developmental functions in some species, but are not found in closely related species, may point to the existence of multiprotein assemblies, which are conserved at the mechanistic level and can function with more than one combination of protein constituents.

  • 185. Sloman, Kathrine A
    et al.
    Lepage, Olivier
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. jämförande fysiologi.
    Rogers, JT
    Wood, Chris M
    Winberg, Svante
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. jämförande fysiologi.
    Socially-mediated differences in brain monoamines in rainbow trout: effects of trace metal contaminants2005Ingår i: AQUATIC TOXICOLOGY, Vol. 71, nr 3, s. 237-247Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Monoaminergic systems play a crucial role in linking behaviour and physiology. Here the physiological and behavioural effects of metal exposure in relation to monoaminergic systems were considered by exposing rainbow trout dyads, demonstrating stable dominance relationships, to cadmium or lead. Fish exposed to 4 mug l(-1) cadmium accumulated more cadmium at the gill than fish held in control water. Fish exposed to 7 mug l(-1) cadmium had higher gill, liver and kidney cadmium concentrations. No significant lead accumulation was seen after exposure to 46 mug l(-1) for 48 h but exposure to 325 mug l(-1) lead caused an increase in gill, liver and kidney lead concentrations. Brain accumulation of both cadmium and lead was only seen after exposure to the highest concentrations. Exposure to 4 or 7 mug l(-1) cadmium, or 46 or 325 mug l(-1) lead for 48 h did not disrupt established dominance hierarchies. As expected with this stable behavioural situation, in control pairs, animals of different social status displayed different physiological profiles. Subordinate fish had higher concentrations of circulating plasma cortisol and telencephalic 5-hydroxyindoleacetic acid/5-hydroxytryptamine (serotonin) (5-HIAA/5-HT) ratios. However, these physiological profiles were affected by metal exposure, with a trend towards higher serotonergic activity in dominant fish. Dominants exposed to 325 mug l(-1) lead had significantly higher hypothalamic 5-HIAA/5-HT ratios when compared with subordinates. The results demonstrate that if stable social hierarchies are established in control water they may not be affected by exposure to cadmium and lead although physiological changes may be evident.

  • 186. Sloman, Kathrine A
    et al.
    McDonald, MD
    Barimo, JF
    Lepage, Olivier
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. jämförande fysiologi.
    Winberg, Svante
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. jämförande fysiologi.
    Wood, Chris M
    Does pulsatile urea excretion serve as a social signal in the gulf toadfish Opsanus beta?2005Ingår i: PHYSIOLOGICAL AND BIOCHEMICAL ZOOLOGY, Vol. 78, nr 5, s. 724-735Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    This study evaluated the hypothesis that the pulsatile excretion of urea by toadfish could serve as a social signal. In the first experiment, physiological parameters were measured in pairs of dominant and subordinate toadfish. Subordinate toadfish had elevated concentrations of circulating plasma cortisol, an effect maintained even after cannulation. In the second experiment, one fish of a pair was injected with C-14-urea, and the occurrence of urea pulses during social encounters was documented. Social status did not influence the order of pulsing, that is, whether a dominant or subordinate fish pulsed first during a social encounter. However, in seven out of eight pairs, both toadfish pulsed within 2 h of each other, indicating some form of communication between fish. In the third and final experiment, the response of toadfish to urea (natural or synthetic) was observed. There was a tendency for toadfish to avoid synthetic urea but there was no apparent behavioural response to water containing toadfish urea. Pulsing events do not appear to play an integral role during social encounters as previously hypothesised, but the close timing of pulses in toadfish pairs suggests some transfer of information.

  • 187.
    Soderhall, K
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. Jämförande fysiologi.
    Thornqvist, PO
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Teknisk-naturvetenskapliga fakulteten, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi. Jämförande fysiologi.
    Crustacean immunity - A short review1997Ingår i: FISH VACCINOLOGY, ISSN 0301-5149, Vol. 90, s. 45-51Artikel i tidskrift (Refereegranskat)
  • 188. Soonthornchai, Wipasiri
    et al.
    Rungrassamee, Wanilada
    Karoonuthaisiri, Nitsara
    Jarayabhand, Padermsak
    Klinbunga, Sirawut
    Söderhäll, Kenneth
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Jiravanichpaisal, Pikul
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för fysiologi och utvecklingsbiologi, Jämförande fysiologi.
    Expression of immune-related genes in the digestive organ of shrimp, Penaeus monodon, after an oral infection by Vibrio harveyi2010Ingår i: Developmental and comparative immunology, ISSN 1879-0089, Vol. 34, nr 1, s. 19-28Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    In all previous studies, to study shrimp immune response, bacteria were directly injected into the shrimp body and as a consequence the initial step of a natural interaction was omitted. In this study we have instead used an immersion technique, which is a more natural way of establishing an infection, to study immune responses in black tiger shrimp (Penaeus monodon). Normally, Vibrio harveyi (Vh) is highly pathogenic to post-larval shrimp, but not to juveniles which usually resist an infection. In post-larvae, Vh causes a massive destruction of the digestive system, especially in the hepatopancreas and in the anterior gut. We have therefore investigated changes in transcription levels of fifteen immune-related genes and morphological changes in juvenile shrimp following an immersion of shrimp in Vh suspension. We found that a pathogenic bacterium, Vh, has the capacity to induce a local expression of some immune-related genes in shrimp after such a bacterial immersion. Our results show that in the juvenile gut small changes in expression of the antimicrobial peptide (AMP) genes such as antilipopolysaccharide factor isoform 3, crustin and penaeidin were observed. However some other genes were more strongly induced in their expression compared to the AMP genes. C-type lectin, Tachylectin 5a1 and mucin-like peritrophic membrane were increased in their expression and the C-type lectin was affected most in its expression. Several other examined genes did not change their expression levels. By performing histology studies it was found that Vh infection induc