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  • 51. Frazier, Ann E
    et al.
    Dudek, Jan
    Guiard, Bernard
    Voos, Wolfgang
    Li, Yanfeng
    Lind, Maria
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. j.
    Meisinger, Chris
    Geissler, Andreas
    Sickmann, Albert
    Meyer, Helmut E
    Bilanchone, Virginia
    Cumsky, Michael G
    Truscott, Kaye N
    Pfanner, Nikolaus
    Rehling, Peter
    Pam16 has an essential role in the mitochondrial protein import motor.2004In: Nat Struct Mol Biol, ISSN 1545-9993, Vol. 11, no 3, p. 226-33Article in journal (Refereed)
  • 52.
    Gambäck Keyser, Pia
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Structure and localisation of some immune proteins in freshwater crayfish1995Licentiate thesis, monograph (Other scientific)
  • 53. Gu, H F
    et al.
    Lind, M I
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Wieslander, L
    Landegren, U
    Söderhäll, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    Melefors, O
    Using PRINS for gene mapping in polytene chromosomes.1997In: Chromosome Res, ISSN 0967-3849, Vol. 5, no 7, p. 463-5Article in journal (Refereed)
  • 54. Hall, M
    et al.
    Scott, T
    Sugumaran, M
    Söderhäll, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    Law, J H
    Proenzyme of Manduca sexta phenol oxidase: purification, activation, substrate specificity of the active enzyme, and molecular cloning.1995In: Proc Natl Acad Sci U S A, ISSN 0027-8424, Vol. 92, no 17, p. 7764-8Article in journal (Refereed)
  • 55. Hall, M
    et al.
    van Heusden, M C
    Söderhäll, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Identification of the major lipoproteins in crayfish hemolymph as proteins involved in immune recognition and clotting.1995In: Biochem Biophys Res Commun, ISSN 0006-291X, Vol. 216, no 3, p. 939-46Article in journal (Refereed)
  • 56. HALL, M
    et al.
    VANHEUSDEN, MC
    SODERHALL, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    IDENTIFICATION OF THE MAJOR LIPOPROTEINS IN CRAYFISH HEMOLYMPH AS PROTEINS INVOLVED IN IMMUNE RECOGNITION AND CLOTTING1995In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ISSN 0006-291X, Vol. 216, no 3, p. 939-946Article in journal (Refereed)
  • 57. Hall, M
    et al.
    Wang, RG
    van, Antwerpen R
    Sottrup-Jensen, L
    Söderhäll, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    The crayfish plasma clotting protein: A vitellogenin-related protein responsible for clot formation in crustacean blood1999In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, ISSN 0027-8424, Vol. 96, no 5, p. 1965-1970Article in journal (Refereed)
    Abstract [en]

    Coagulation in crayfish blood is based on the transglutaminase-mediated crosslinking of a specific plasma clotting protein. Here we report the cloning of the subunit of this clotting protein from a crayfish hepatopancreas cDNA library. The ORF encodes a p

  • 58. Hernandez-Cortes, Patricia
    et al.
    Cerenius, Lage
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Garcia-Carreno, F
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Trypsin from Pacifastacus leniusculus hepatopancreas: Purification and cDNA cloning of the synthesized zymogen1999In: Biological chemistry (Print), ISSN 1431-6730, E-ISSN 1437-4315, Vol. 380, no 4, p. 499-501Article in journal (Refereed)
    Abstract [en]

    Trypsin was purified from crayfish, Pacifastacus leniusculus, hepatopancreas, and the gene that encoded this enzyme was cloned from a hepatopancreas cDNA library. Crayfish trypsin is synthesized as a zymogen according to the sequence of the putative precu

  • 59. Hernroth, Bodil
    et al.
    Baden, Susanne P
    Holm, Kristina
    André, Tove
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. j.
    Söderhäll, Irene
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    Manganese induced immune suppression of the lobster, Nephrops norvegicus2004In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 70, no 3, p. 223-231Article in journal (Refereed)
    Abstract [en]

    Manganese (Mn) is one of the most abundant elements on earth, particularly in the soft bottom sediments of the oceans. As a micronutrient Mn is essential in the metabolic processes of organisms. However, at high concentrations the metal becomes a neurotoxin with well-documented effects. As a consequence of euthrophication, manganese is released from bottom sediments of coastal areas and the Norway lobsters, Nephrops norvegicus, can experience high levels of bioavailable Mn2+. Here, we present the first report showing that Mn also affects several fundamental processes in the mobilisation and activation of immunoactive haemocytes. When N. norvegicus was exposed to a realistic [Mn2+] of 20 mg l(-1) for 10 days 24.1 mug ml(-1) was recorded in the haemolymph. At this concentration the total haemocyte count was reduced by ca. 60%. By using BrdU as a tracer for cell division, it was shown that the proliferation rate in the haematopoietic tissue did not increase, despite the haemocytepenia. A gene coding for a Runt-domain protein, known to be involved in maturation of immune active haemocytes in a variety of organisms, was identified also in haemocytes of N. norvegicus. The expression of this gene was >40% lower in the Mn-exposed lobsters as judged by using a c DNA probe and the in situ hybridisation technique. In response to non-self molecules, like lipopolysaccharide, (LPS), the granular haemocytes of arthropods are known to degranulate and thereby release and activate the prophenoloxidase, system, necessary for their immune defence. A degranulation assay, tested on isolated granular haemocytes, showed about 75% lower activity in the Mn-exposed lobsters than that for the unexposed. Furthermore, using an enzymatic assay, the activation per se of prophenoloxidase by LPS was found blocked in the Mn-exposed lobsters. Taken together, these results show that Mn exposure suppressiA fundamental immune mechanisms of Norway lobsters. This identifies a potential harm that also exists for other organisms and should be considered when increasing the distribution of bioavailable Mn, as has been done through recently introduced applications of the metal.

  • 60. Holmblad, T
    et al.
    Söderhäll, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Cell adhesion molecules and antioxidative enzymes in a crustacean, possible role in immunity1999In: AQUACULTURE, ISSN 0044-8486, Vol. 172, no 1-2, p. 111-123Article in journal (Refereed)
    Abstract [en]

    The question as to how the immune defence of an invertebrate animal is initiated and coordinated has largely been unanswered. This short review focuses on recent discoveries about crayfish hemolymph proteins, which may play roles in cell adhesion events l

  • 61. Holmblad, T
    et al.
    Thornqvist, PO
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Soderhall, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Johansson, MW
    Identification and cloning of an integrin beta subunit from hemocytes of the freshwater crayfish Pacifastacus leniusculus1997In: Journal of Experimental Zoology, ISSN 0022-104X, Vol. 277, no 3, p. 255-261Article in journal (Other scientific)
    Abstract [en]

    We have cloned and sequenced a beta subunit of integrin from a cDNA library of crayfish hemocytes. This beta integrin shows great similarity to beta integrin subunits from other animals; the highest is towards beta pat-3 from Caenorhabditis elegans follow

  • 62.
    Holmblad, Torbjörn
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Anti-oxidative enzymes and cell adhesion molecules in crayfish hemocytes.1997Licentiate thesis, monograph (Other scientific)
  • 63. Huang, Chih-Cheng
    et al.
    Sritunyalucksana, Kallaya
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Söderhäll, Kenneth
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Song, Yen-Ling
    Molecular cloning and characterization of tiger shrimp (Penaeus monodon) transglutaminase.2004In: Dev Comp Immunol, ISSN 0145-305X, Vol. 28, no 4, p. 279-94Article in journal (Refereed)
  • 64. Huang, TS
    et al.
    Law, JH
    Soderhall, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Purification and cDNA cloning of ferritin from the hepatopancreas of the freshwater crayfish Pacifastacus leniusculus1996In: EUROPEAN JOURNAL OF BIOCHEMISTRY, ISSN 0014-2956, Vol. 236, no 2, p. 450-456Article in journal (Refereed)
    Abstract [en]

    Ferritin was purified from the hepatopancreas of the freshwater crayfish Pacifastacus leniusculus after injection of iron. It has the same size as horse spleen ferritin (440 kDa) and migrates as two bands, 19 kDa and 20 kDa, respectively, in SDS/PAGE unde

  • 65. Huang, TS
    et al.
    Melefors, O
    Lind, Maria
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Söderhäll, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    An atypical Iron-Responsive Element (IRE) within crayfish ferritin mRNA and an Iron Regulatory Protein 1 (IRP1)-like protein from crayfish hepatopancreas1999In: INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, ISSN 0965-1748, Vol. 29, no 1, p. 1-9Article in journal (Refereed)
    Abstract [en]

    A putative crayfish iron-responsive element (IRE) is present in the 5'-untranslated region of the crayfish ferritin mRNA. The putative crayfish IRE is in a cap-proximal position and shares most of the structural features of the consensus IRE, but the RNA

  • 66.
    Huang, TS
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Wang, HY
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Lee, SY
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Johansson, MW
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Cerenius, Lage
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    A cell adhesion protein from the crayfish Pacifastacus leniusculus, a serine proteinase homologue similar to Drosophila masquerade2000In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 275, no 14, p. 9996-10001Article in journal (Refereed)
    Abstract [en]

    A cDNA encoding a protein resembling masquerade, a serine proteinase homologue expressed during embryogenesis, larval, and pupal development in Drosophila melanogaster, was identified in hemocytes of the adult freshwater crayfish, Pacifastacus leniusculus. The crayfish protein is similar to Drosophila masquerade in the following aspects: (a) overall sequence of the serine proteinase domain, such as the position of three putative disulfide bridges, glycine in the place of the catalytic serine residue, and the presence of a substrate-lining pocket typical for trypsins; (b) the presence of several copies of a disulfide-knotted motif in the putative propeptide. This masquerade-like protein is cleaved into a 27-kDa fragment, which could be detected by immunoblot analysis using an affinity-purified antibody against a synthetic peptide in the C-terminal domain of the protein. The 27-kDa protein could be immunoaffinity-purified from hemocyte lysate supernatant and exhibited cell adhesion activity in vitro, indicating that the C-terminal domain of the crayfish masquerade-like protein mediates cell adhesion.

  • 67.
    Höglund, E
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Evolutionary Biology. Department of Physiology and Developmental Biology, Comparative Physiology.
    Blam, PHM
    Winberg, S
    Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Behavioural and neuroendocrine effects of environmental background colour and social interaction in Arctic charr (Salvelinus alpinus).2002In: Journal of Experimental Biology, Vol. 205, p. 2535-2543.Article in journal (Refereed)
  • 68.
    Höglund, E
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Evolutionary Biology. Department of Physiology and Developmental Biology, Comparative Physiology.
    Blam, PHM
    Winberg, S
    Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Stimulatory and inhibitory effects of 5-HT1A receptors on ACTH and cortisol secretion in a teleost fish, the Arctic charr (Salvelinus alpinus).2002In: Neuroscience Letters, Vol. 324, p. 193-196Article in journal (Refereed)
  • 69. Höglund, Erik
    et al.
    Bakke, Marit J
    Överli, Öyvind
    Winberg, Svante
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    Nilsson, Göran E
    Suppression of aggressive behaviour in juvenile Atlantic cod (Gadus morhua) by L-tryptophan supplementation2005In: AQUACULTURE, Vol. 249, no 1-4, p. 525-531Article in journal (Refereed)
    Abstract [en]

    Aggressive interactions and cannibalism of juvenile Atlantic cod may cause substantial production losses under conditions of intensive rearing. In other teleosts, chronically increased brain concentrations and turnover of the neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) are associated with suppressed aggression. Further, dietary supplementation with the serotonin precursor, L-tryptophan (TRP) suppresses aggression in juvenile rainbow trout (Onchorhyncus mykiss) and reduces cannibalism in juvenile grouper (Epinephelus coioides). In the present study, the behavioural effect of dietary TRP supplementation was observed in pairs of juvenile Atlantic cod subjected to repeated encounters for 10 days. After 3 days, one group was given TRP-supplemented feed (28 g/kg). A significant (P < 0.01) decrease in aggressive acts (from 40 +/- 2.7/ 10 min to 17 +/- 2.0/10 min, mean +/- standard error of mean) was seen after changing to TRP-supplemented feed. The mean number of aggressive acts was also significantly (P<0.05) lower in the TRP-treated group (17 +/- 2.0/10 min) compared to a control group not receiving TRP treatment (29 3.3/10 min). A second experiment examined the effect of TRP-supplemented feed (28 g/kg) on the activity of central 5-HT, quantified as the concentration ratio between the 5-HT metabolite, 5-hydroxyindoleacetic acid (5-HIAA), and 5-HT. In this experiment, the TRP-treated group ([5-HIAA]/[5-HT]=0.56 +/- 0.04) showed elevated values (P<0.038) compared with the control group ([5-HIAA]/[5-HT]=0.43 +/- 0.03). In conclusion, this study shows that juvenile Atlantic cod are highly aggressive and that supplementing the feed with TRP affects central 5-HT signalling systems and reduces this behaviour. (c) 2005 Elsevier B.V. All rights reserved.

  • 70.
    Höglund, Erik
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Evolutionary Biology. Department of Physiology and Developmental Biology, Comparative Physiology.
    Balm, PHM
    Winberg, Svante
    Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Skin darkening, a potential social signal in subordinate Arctic charr (Salvelinus alpinus): The regulatory role of brain monoamines and pro-opiomelanocortin-derived peptides2000In: JOURNAL OF EXPERIMENTAL BIOLOGY, ISSN 0022-0949, Vol. 203, no 11, p. 1711-1721Article in journal (Refereed)
    Abstract [en]

    Arctic charr were allowed to interact in groups of three for 5 days. Skin darkness was quantified by measuring the mean brightness of individual fish before and after social interaction. Brain levels of monoamines and monoamine metabolites and plasma conc

  • 71.
    Höglund, Erik
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolutionary Biology, Limnology.
    Kolm, Niclas
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolutionary Biology, Animal Ecology.
    Winberg, Svante
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Stress-induced changes in brain serotonergic activity, plasma cortisol and aggressive behavior in Arctic charr (Salvelinus alpinus) is counteracted by L-DOPA2001In: Physiology and Behavior, ISSN 0031-9384, E-ISSN 1873-507X, Vol. 74, no 3, p. 381-389Article in journal (Refereed)
    Abstract [en]

    Arctic charr (Salvelinus alpinus) were tested for aggressive behavior using intruder tests, before and after 2 days of dyadic social interaction. Following social interaction, half of the dominant and half of the subordinate fish were given l-DOPA (10 mg/kg, orally), whereas the remaining dominant and subordinate fish were given vehicle. One hour following drug treatment, the fish were tested for aggressive behavior again in a third and final intruder test, after which blood plasma and brain tissue were sampled for analysis of plasma cortisol concentrations and brain levels of monoamines and monoamine metabolites. Subordinate fish showed a reduction in the number of attacks launched against the intruder, as well as an increase in attack latency, as compared to prior to dyadic social interactions. Social subordination also resulted in an elevation of brain serotonergic activity. Fish receiving l-DOPA prior to the final intruder test showed shorter attack latency than vehicle controls. Drug treatment was a stressful experience and vehicle controls showed elevated plasma cortisol levels and longer attack latency as compared to before treatment. l-DOPA-treated fish showed lower plasma levels of cortisol and lower serotonergic activity in certain brain areas than vehicle controls. These results suggest that l-DOPA counteracts the stress-induced inhibition of aggressive behavior, and at the same time inhibits stress-induced effects on brain serotonergic activity and plasma cortisol concentrations.

  • 72. Höglund, Erik
    et al.
    Weltzien, FA
    Schjolden, Joachim
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    Winberg, Svante
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    Ursin, Holger
    Döving, Kjell B
    Avoidance behavior and brain monoamines in fish2005In: BRAIN RESEARCH, Vol. 1032, no 1-2, p. 104-110Article in journal (Refereed)
    Abstract [en]

    The crucian carp performs a typical avoidance behavior when exposed to olfactory cues from injured skin of conspecifics. They swim rapidly to the bottom and hide in available material. This work examines the effects of skin extract exposure and availability of hiding material on this behavior, and concomitant changes in brain monoaminergic activity in crucian carp. Individual fish were exposed to skin extract in aquaria with or without hiding material. Exposure to skin extract resulted in the expected avoidance behavior consisting of rapid movement towards the bottom of the aquarium. This lasted for 1-2 min. Activity then decreased below the level observed before exposure, suggesting a "freezing" type of avoidance behavior. This behavior was independent of availability of hiding material.

    Brain dopaminergic activity increased in telencephalon and decreased in the brain stem following skin extract exposure, again independent of availability of hiding material. However, fish kept in aquaria without hiding material showed an elevation of serotonergic activity in the brain stem and the optic tectum compared to fish with available hiding material. Absence of hiding material increased serotonergic activity also without exposure to skin extract. In aquaria with hiding material, the fish stirred up a cloud of fine sediments and showed a more pronounced decrease in locomotor activity in agreement with this being a more efficient freezing or immobile avoidance behavior. These results show that basic components of avoidance behavior and related brain changes are present in the fish brain, in accordance with the common phylogenetic roots of avoidance behavior in all vertebrates. (C) 2004 Elsevier B.V. All rights reserved.

  • 73. Jiang, Q
    et al.
    Hall, M
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Noriega, F G
    Wells, M
    cDNA cloning and pattern of expression of an adult, female-specific chymotrypsin from Aedes aegypti midgut.1997In: Insect Biochem Mol Biol, ISSN 0965-1748, Vol. 27, no 4, p. 283-9Article in journal (Refereed)
  • 74. Jimenez-Vega, Florinda
    et al.
    Vargas-Albores, Francisco
    Söderhäll, Kenneth
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Characterisation of a serine proteinase from Penaeus vannamei haemocytes.2005In: Fish Shellfish Immunol, ISSN 1050-4648, Vol. 18, no 2, p. 101-8Article in journal (Refereed)
  • 75.
    Jimenez-Vega, Florinda
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Yepiz-Plascencia, Gloria
    Söderhäll, Kenneth
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Vargas-Albores, Francisco
    A single WAP domain-containing protein from Litopenaeus vannamei hemocytes.2004In: Biochem Biophys Res Commun, ISSN 0006-291X, Vol. 314, no 3, p. 681-7Article in journal (Refereed)
  • 76.
    Jiravanichpaisal, P
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Bangyeekhun, E
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Söderhäll, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Söderhäll, I
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    Experimental infection of white spot syndrome virus in freshwater crayfish Pacifastacus leniusculus.2001In: Dis Aquat Organ, ISSN 0177-5103, Vol. 47, no 2, p. 151-7Article in journal (Refereed)
  • 77.
    Jiravanichpaisal, Pikul
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    White Spot Syndrome Virus Interaction with a Freshwater Crayfish2005Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Viruses are very abundant in water and hence diseases caused by viruses are common in marine organisms. These diseases create great problems for the commercial farming of crustaceans and mussels. One of the most common and most disastrous diseases for shrimp is caused by the white spot syndrome virus (WSSV), which is spread all around the world and also is infecting many different species of crustaceans including freshwater crayfish. Although during recent years knowledge has been gathered on the ways in which invertebrates defend themselves against bacteria and fungi virtually nothing is known about the defence processes elicited by virus. The aim of this work was to develop a model to use for studies of virus-host interactions in vivo and in vitro.

    Temperature was found to be important for the virus infectivity and at lower temperature the virus apparently did not replicate, but if animals kept at low temperature for more than 40 days were transferred to higher temperatures they died quickly due to an increased virus replication. In crayfish infected with the virus it was found that hemocytes did not degranulate and the melanization reaction was also inhibited in the hemocyes. Thus it is apparent that this virus interacts with the immune system and hemocytes in particular and to be able to study this in some greater detail it was necessary to develop a cell culture to study virus-host interactions at the molecular level. Hence, we have developed a stem cell culture from the hematopoietic tissue (hpt) that will differentiate and mature into hemocytes and which can be used to replicate the WSSV in the presence of an endogenous cytokine, astakine. Astakine is the first cytokine like-factor described which is directly involved in hematopoiesis in an invertebrate.

    List of papers
    1. Experimental infection of white spot syndrome virus in freshwater crayfish Pacifastacus Leniusculus
    Open this publication in new window or tab >>Experimental infection of white spot syndrome virus in freshwater crayfish Pacifastacus Leniusculus
    2001 (English)In: Diseases of Aquatic Organisms, ISSN 0177-5103, Vol. 47, p. 151-157Article in journal (Refereed) Published
    Identifiers
    urn:nbn:se:uu:diva-92990 (URN)
    Available from: 2005-04-14 Created: 2005-04-14 Last updated: 2009-04-05Bibliographically approved
    2. WSSV interaction with crayfish hemocytes
    Open this publication in new window or tab >>WSSV interaction with crayfish hemocytes
    (English)Manuscript (Other (popular science, discussion, etc.))
    Identifiers
    urn:nbn:se:uu:diva-92991 (URN)
    Available from: 2005-04-14 Created: 2005-04-14 Last updated: 2010-01-14Bibliographically approved
    3. Effect of water temperature on the immune response and infectivity pattern of white spot syndrome virus (WSSV) in freshwater crayfish
    Open this publication in new window or tab >>Effect of water temperature on the immune response and infectivity pattern of white spot syndrome virus (WSSV) in freshwater crayfish
    2004 (English)In: Fish and Shellfish Immunology, ISSN 1050-4648, Vol. 17, p. 265-275Article in journal (Refereed) Published
    Identifiers
    urn:nbn:se:uu:diva-92992 (URN)
    Available from: 2005-04-14 Created: 2005-04-14 Last updated: 2009-04-05Bibliographically approved
    4. An ancient role for a prokineticin domain in invertebrate hematopoiesis
    Open this publication in new window or tab >>An ancient role for a prokineticin domain in invertebrate hematopoiesis
    Show others...
    2005 (English)In: Journal of Immunology, ISSN 0022-1767, E-ISSN 1550-6606, Vol. 174, no 10, p. 6153-6160Article in journal (Refereed) Published
    Abstract [en]

    Hemopoietic development requires firm control of cell proliferation and differentiation. Although recent research has revealed conserved function of transcription factors and signaling pathways regulating lineage commitment in hemopoietic development in Drosophila melanogaster and vertebrates, little is known about hemopoietic cytokines among the invertebrate phyla. In the present study, we show that differentiation and growth of hemopoietic stem cells in vitro from an invertebrate, Pacifastacus leniusculus, require an endogenous cytokine-like factor, astakine, containing a prokineticin (PK) domain. Astakine induces a strong hematopoiesis response in live animals. An astakine homologue was also found in the shrimp, Penaeus monodon. So far, PK domains are only identified in vertebrates, in which they, for example, direct angiogenic growth. Our finding of the first PK-like cytokine characterized from any invertebrate provides novel information concerning the evolution of growth factors and blood cell development.

    Keywords
    Amino Acid Sequence, Animals, Astacoidea, Base Sequence, Cell Differentiation/physiology, Cell Division/physiology, Cell Movement/physiology, Cells; Cultured, Cloning; Molecular, Cytokines/genetics/*isolation & purification/*physiology/secretion, Cytoplasmic Granules/secretion, Evolution; Molecular, Hematopoiesis/genetics/*physiology, Hematopoietic Stem Cells/cytology/physiology, Hemocytes/cytology/physiology/secretion, Molecular Sequence Data, Penaeidae, Protein Structure; Tertiary/genetics, Research Support; Non-U.S. Gov't, Sequence Homology; Amino Acid, Structural Homology; Protein, Vascular Endothelial Growth Factor; Endocrine-Gland-Derived/chemistry/genetics/isolation & purification/*physiology/secretion
    National Category
    Natural Sciences
    Identifiers
    urn:nbn:se:uu:diva-92993 (URN)15879111 (PubMedID)
    Available from: 2005-04-14 Created: 2005-04-14 Last updated: 2017-12-14
    5. Replication of white spot syndrome virus (WSSV) in in vitro cultured hematopoietic cell of freshwater crayfish Pacifastacus leniusculus
    Open this publication in new window or tab >>Replication of white spot syndrome virus (WSSV) in in vitro cultured hematopoietic cell of freshwater crayfish Pacifastacus leniusculus
    (English)Manuscript (Other (popular science, discussion, etc.))
    Identifiers
    urn:nbn:se:uu:diva-92994 (URN)
    Available from: 2005-04-14 Created: 2005-04-14 Last updated: 2010-01-14Bibliographically approved
  • 78.
    Jiravanichpaisal, Pikul
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Bangyeekhun, Eakaphun
    Söderhäll, Kenneth
    Söderhäll, Irene
    Experimental infection of white spot syndrome virus in freshwater crayfish Pacifastacus Leniusculus2001In: Diseases of Aquatic Organisms, ISSN 0177-5103, Vol. 47, p. 151-157Article in journal (Refereed)
  • 79.
    Jiravanichpaisal, Pikul
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Lee, Bok Luel
    Söderhäll, Kenneth
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Cell-mediated immunity in arthropods: Hematopoiesis, coagulation, melanization and opsonization.2006In: Immunobiology, ISSN 0171-2985, Vol. 211, no 4, p. 213-36Article in journal (Refereed)
  • 80.
    Jiravanichpaisal, Pikul
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Lee, So-Young
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Kim, Young-A
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Andrén, Tove
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Irene
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Antibacterial peptides in hemocytes and hematopoietic tissue from freshwater crayfish Pacifastacus leniusculus: Characterization and expression pattern2007In: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 31, no 5, p. 441-455Article in journal (Refereed)
    Abstract [en]

    A 14 amino acid residues proline/arginine-rich antibacterial peptide designated as astacidin 2 was purified and characterized from hemocytes of the freshwater crayfish, Pacifastacus leniusculus. Astacidin 2 has a broad range of antibacterial activity against both Gram-positive and Gram-negative bacteria. The primary sequence of astacidin 2 is RPRPNYRPRPIYRP with an amidated C-terminal and the molecular mass is 1838 Da determined by mass spectrometry. Furthermore, the cDNA of three different crustin antibacterial homologs were isolated from a crayfish hemocyte EST library. RT-PCR was used to analyze the expression of the genes coding for astacidin 2 and P. leniusculus crustins (Plcrustin) 1–3 after bacterial challenge. The expression of Plcrustin1 was upregulated in both hemocytes and hematopoietic tissue after challenge with Gram-negative Escherichia coli or Acinetobacter ssp. non pathogenic bacteria as well as by a Gram negative crayfish pathogenic bacterium (Aeromonas hydrophila). The PlCrustin3 transcript was only upregulated after inoculation with the non-pathogenic Acinetobacter ssp. while there was no change in expression of Plcrustin2 or astacidin 2 following a bacterial challenge.

  • 81.
    Jiravanichpaisal, Pikul
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Puanglarp, Narongsak
    Petkon, Sasithon
    Donnuea, Seri
    Söderhäll, Irene
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Expression of immune-related genes in larval stages of the giant tiger shrimp, Penaeus monodon2007In: Fish and Shellfish Immunology, ISSN 1050-4648, E-ISSN 1095-9947, Vol. 23, no 4, p. 815-824Article in journal (Refereed)
    Abstract [en]

    Shrimp undergo several morphologically different stages during development and therefore the expression of some immune-related genes such as prophenoloxidase (proPO), peroxinectin (Prx), crustin (Crus), penaeidin (Pen), transglutaminase (TGase), haemocyanin (Hc) and astakine (Ak) were determined during larval development of the shrimp (Penaeus monodon), i.e. nauplius 4 (N4), protozoea 1 and 3 (Z1 and 3), mysis 3 (My 3), post-larvae 3 (PL3) and also in haemocytes of juveniles. Semi-quantitative RT-PCR analysis showed that all transcripts were already present in the early larval stage of N4 but at different levels. The transcript of proPO was found to be extremely low or even absent at N4, whereas Prx, Crus, Pen, TGase, Hc and Ak were significantly expressed at all larval stages. Up to now expression of proPO and Prx has only been reported from haemocytes in crustaceans and in this study Prx also appeared to be expressed in stages which appear to lack haemocytes. Thus, this may suggest that Prx is expressed in other cells than haemocytes. It is well known among invertebrates that the proPO system plays a crucial role as an immune effector molecule against microbes. However, in this study, the transcript of proPO was low during the larval stages and hardly present at all at N4. This might indicate that the development of immune-competent haemocytes during the larval stages is not completed and as a consequence they are likely to be more susceptible to infectious diseases during these stages.

  • 82.
    Jiravanichpaisal, Pikul
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Roos, Stefan
    Edsman, Lennart
    Liu, Haipeng
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    A highly virulent pathogen, Aeromonas hydrophila, from the freshwater crayfish Pacifastacus leniusculus2009In: Journal of Invertebrate Pathology, ISSN 0022-2011, E-ISSN 1096-0805, Vol. 101, no 1, p. 56-66Article in journal (Refereed)
    Abstract [en]

    Aeromonas spp. are characteristic bacteria of freshwaters and many of them can be components of the bacterial flora of aquatic animals and may become pathogens on animals including humans. In this study Aeromonas hydrophila was isolated from the freshwater crayfish, Pacifastacus leniusculus, and was found to be a highly pathogenic bacterium among many isolated bacteria. Mortality reached 100% within 6h when 200 microl of 1.24 x 10(7)CFU/ml was applied by injection. Histopathological studies of moribund crayfish showed that extensive necrotic nuclei and clump-infiltrated hemocytes were found in observed tissues including gill, heart, hepatopancreas and the circulatory system. To verify how crayfish are susceptible to this bacterium, crude extracellular products (ECPs) obtained from culture supernatant of A. hydrophila was studied either in vivo or in vitro. ECPs (200 microl) were able to kill crayfish by injection. In an in vitro study, ECPs induced cytotoxicity of hemocytes as well as hematopoietic cells in a dose- and time-dependent manner after 30 min post inoculation. Two genes coding for endotoxins were also found in this isolate of A. hydrophila. This indicates that the bacterial endotoxins are the causative agents of crayfish mortality. Moreover, the effect of temperature on the infectivity of A. hydrophila to crayfish was also studied. At 4 degrees C, all crayfish survived, whereas at 20 degrees C the animals died rapidly after bacterial challenge. At this low temperature A. hydrophila did not replicate or replicated at a very low degree and hence crayfish could probably mount effective cellular reactions towards A. hydrophila.

  • 83.
    Jiravanichpaisal, Pikul
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Sricharoen, Siripavee
    Söderhäll, Irene
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Söderhäll, Kenneth
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    White spot syndrome virus (WSSV) interaction with crayfish haemocytes.2006In: Fish Shellfish Immunol, ISSN 1050-4648, Vol. 20, no 5, p. 718-27Article in journal (Refereed)
  • 84.
    Jiravanichpaisal, Pikul
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Sricharoen, Siripavee
    Söderhäll, Irene
    Söderhäll, Kenneth
    WSSV interaction with crayfish hemocytesManuscript (Other (popular science, discussion, etc.))
  • 85.
    Jiravanichpaisal, Pikul
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    Söderhäll, Kenneth
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    Pathogens, parasites and ectocommensals: Viruses2006In: Atlas of crayfish in Europe, Publications Scientifiques du MNHN, Paris , 2006Chapter in book (Refereed)
  • 86.
    Jiravanichpaisal, Pikul
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Irene
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Characterization of white spot syndrome virus replication in in vitro-cultured haematopoietic stem cells of freshwater crayfish, Pacifastacus leniusculus2006In: Journal of General Virology, ISSN 0022-1317, E-ISSN 1465-2099, Vol. 87, no Pt 4, p. 847-854Article in journal (Refereed)
    Abstract [en]

    Replication of White spot syndrome virus (WSSV) was investigated in haematopoietic cells (hpt cells) derived from haematopoietic tissue (hpt) of freshwater crayfish, Pacifastacus leniusculus. Temperature and type of inoculum for virus replication were studied. The cell culture remained viable at a wide range of temperatures ranging from 4 to 25 degrees C. WSSV replicated in cells, as evidenced by in situ hybridization, RT-PCR and by the presence of virions visualized with an electron microscope. Moreover, the results showed that the infectivity of WSSV to hpt cells is dependent on temperature and a supplemented growth factor (cytokine) astakine. WSSV replicated more rapidly at higher temperatures than at lower temperatures. No virus replication was observed at 4 degrees C. Detectable WSSV-infected cells were present as early as 36 In post-inoculation, demonstrated by in situ hybridization or RT-PCR of VP28 expression at 25 degrees C. Hot cells can survive a few weeks at 25 or 16 degrees C and longer than several months at 4 degrees C.

  • 87.
    Jiravanichpaisal, Pikul
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Söderhäll, Irene
    Effect of water temperature on the immune response and infectivity pattern of white spot syndrome virus (WSSV) in freshwater crayfish2004In: Fish and Shellfish Immunology, ISSN 1050-4648, Vol. 17, p. 265-275Article in journal (Refereed)
  • 88.
    Jiravanichpaisal, Pikul
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Söderhäll, Irene
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Effect of water temperature on the immune response and infectivity pattern of white spot syndrome virus (WSSV) in freshwater crayfish.2004In: Fish Shellfish Immunol, ISSN 1050-4648, Vol. 17, no 3, p. 265-75Article in journal (Refereed)
  • 89.
    Jiravanichpaisal, Pikul
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Söderhäll, Irene
    Replication of white spot syndrome virus (WSSV) in in vitro cultured hematopoietic cell of freshwater crayfish Pacifastacus leniusculusManuscript (Other (popular science, discussion, etc.))
  • 90.
    Joachim, Schjolden
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    Stoskhus, Argaudas
    Winberg, Svante
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    Does individual variation in stress responses and agonistic behavior reflect divergent stress coping strategies in juvenile rainbow trout?2005In: PHYSIOLOGICAL AND BIOCHEMICAL ZOOLOGY, Vol. 78, no 5, p. 715-723Article in journal (Other (popular scientific, debate etc.))
    Abstract [en]

    Individual rainbow trout were transferred to visual isolation in experimental aquaria. As a measure of the speed of acclimation, individual food intake was quantified during the first 6 d following transfer. Following acclimation, aggression was quantified by subjecting the fish to three resident-intruder tests, with 30 d of recovery between the tests. Moreover, between the resident-intruder tests (i.e., two times) the fish were exposed to an unfamiliar environment and their cortisol response was measured. The results of this study show that individuals of juvenile rainbow trout differ distinctly in their response to changes in their environment, and that this diversity in behavior is reflected by consistent behavioral traits displayed by individual fish. These traits have proven to be consistent not only over time but also across situations, revealing two distinct behavioral profiles, in the same manner as shown in studies on proactive and reactive mammals. Our results also show that the reactivity of the hypothalamic-pituitary-interrenal (HPI) axis, when exposed to a stressor, is a consistent physiological trait in juvenile rainbow trout. We found that difference in HPI axis reactivity is linked to the different behavioral profiles. However, HPI axis reactivity could not be linked directly to the singular behavioral traits measured. In other words, we did not find that the consistent behavioral traits shown by the fish were associated with a difference in HPI axis reactivity in the same manner as the reactivity of the hypothalamic-pituitary-adrenocortical axis does in mammals. Taken together, our results show that stress coping strategies akin to what has been described as reactive and proactive stress coping in mammals appear to exist in juvenile rainbow trout.

  • 91. Jobling, M
    et al.
    Koskela, J
    Winberg, S
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Feeding and growth of whitefish fed restricted and abundant rations: influences on growth heterogeneity and brain serotonergic activity1999In: JOURNAL OF FISH BIOLOGY, ISSN 0022-1112, Vol. 54, no 2, p. 437-449Article in journal (Refereed)
    Abstract [en]

    The whitefish, Coregonus lavaretus, is a schooling species, and it was hypothesized that, when faced with feed restriction, the behaviours associated with a schooling habit would lead to there being less heterogeneity in feeding and growth than has been o

  • 92.
    Johansson, Karin
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Interaction Between Drosophila melanogaster mbn-2 Cells and Bacteria2005Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Innate immunity relies on a repertoire of germline-encoded non-rearranging pattern recognition receptors that bind to invariant microbial surface molecules. This event initiates a number of signal transduction cascades that lead to humoral and cellular defense responses like synthesis of antimicrobial peptides, phagocytosis and coagulation – mechanisms that efficiently fight infectious microorganisms and have been evolutionary conserved to exist in parallel with the antibody-based adaptive immunity found in vertebrates.

    The fruit fly, Drosophila melanogaster represents a widely used animal model for studies of a pristine innate immune system. Its immune responsive intracellular signalling pathways display a high degree of similarity with the NF-κB /Rel-signalling pathways that mediate the inflammatory response in mammals. Insects are also vectors for medically important parasitic diseases which can trigger immune responses in the vector so basal knowledge about the regulation and function of insect immune systems can contribute to our understanding of inflammation and microbial disease in higher animals and open new strategies for biological vector control.

    Drosophila hemocytes play a key role in executing and coordinating local and systemic defenses in response to infection. This thesis describes in vitro studies of Drosophila gene expression in response to bacterial infection using the larval hemocyte-like cell line – mbn-2. Our results show that immune challenge with bacterial cell wall components and intact live bacteria induces differential gene expression that gives clues to how cellular immune responses could be activated and regulated.

    List of papers
    1. Enteric bacteria counteract lipopolysaccharide induction of antimicrobial peptide genes
    Open this publication in new window or tab >>Enteric bacteria counteract lipopolysaccharide induction of antimicrobial peptide genes
    Show others...
    2001 (English)In: Journal of Immunology, ISSN 0022-1767, Vol. 167, p. 6920-6923Article in journal (Refereed) Published
    Identifiers
    urn:nbn:se:uu:diva-92568 (URN)
    Available from: 2005-02-11 Created: 2005-02-11 Last updated: 2009-04-05Bibliographically approved
    2. Microarray analysis of immune challenged Drosophila hemocytes
    Open this publication in new window or tab >>Microarray analysis of immune challenged Drosophila hemocytes
    2005 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 305, no 1, p. 145-155Article in journal (Refereed) Published
    Abstract [en]

    nsect hemocytes play multiple roles in immunity and carry out cellular responses like phagocytosis, encapsulation and melanization as well as producing humoral effector proteins in the first line of defense after injury and invasion of microorganisms. In this work, we used the Drosophila melanogaster hemocyte-like cell line mbn-2 and Affymetrix Drosophila GeneChips to investigate the transcriptome of a single type of immune competent tissue exposed to Gram-negative cell wall components (crude LPS) or high dose infection with live Escherichia coli. We found that gene expression profiles of both treatments overlap but show important differences in expression levels of several genes involved in immunity. In addition, cell morphology during infection was monitored and revealed distinct alterations in cell shape and adhesion. Presence of large numbers of bacteria also increased the number of cells taking on crystal cell fate. Taken together, our results indicate that hemocytes sense and respond differently to purified bacterial surface molecules and infection with live and actively growing bacteria both at the level of gene expression and in cell behavior.

    Keywords
    Animals, Cell Line, Drosophila Proteins/genetics, Drosophila melanogaster/*genetics/immunology, Escherichia coli, Gene Expression Regulation, Gram-Negative Bacteria/immunology, Hemocytes/*immunology, Lipopolysaccharides/toxicity, Microscopy; Electron; Scanning, Oligonucleotide Array Sequence Analysis/*methods, Research Support; Non-U.S. Gov't
    National Category
    Natural Sciences
    Identifiers
    urn:nbn:se:uu:diva-92569 (URN)10.1016/j.yexcr.2004.12.018 (DOI)15777795 (PubMedID)
    Available from: 2005-02-11 Created: 2005-02-11 Last updated: 2017-12-14Bibliographically approved
    3. Diptericin expression in bacteria infected Drosophila mbn-2 cells - effect of infection dose and phagocytosis
    Open this publication in new window or tab >>Diptericin expression in bacteria infected Drosophila mbn-2 cells - effect of infection dose and phagocytosis
    (English)Manuscript (Other (popular science, discussion, etc.))
    Identifiers
    urn:nbn:se:uu:diva-92570 (URN)
    Available from: 2005-02-11 Created: 2005-02-11 Last updated: 2010-10-11Bibliographically approved
    4. Pefabloc: A sulfonyl fluoride serine protease inhibitor blocks induction of Diptericin in Drosophila l(2)mbn cells
    Open this publication in new window or tab >>Pefabloc: A sulfonyl fluoride serine protease inhibitor blocks induction of Diptericin in Drosophila l(2)mbn cells
    2012 (English)In: Insect Science, ISSN 1672-9609, E-ISSN 1744-7917, Vol. 19, no 4, p. 472-476Article in journal (Refereed) Published
    Abstract [en]

    Insects protect themselves against microbial infection by an efficient innate immune system that is activated by recognition of invariant microbial surface molecules. In the fruit fly Drosophila melanogaster the presence of bacteria is associated with expression of antimicrobial peptides in host immune-competent tissues. Host receptors detect infection and relay the signal to mount the appropriate immune response. In Drosophila hemocyte-like l(2)mbn cells pre-infection treatment with Pefabloc, a commonly used serine protease inhibitor, induced two major effects: it blocked expression of the antibacterial peptide Diptericin in response to live Gram-negative bacteria and bacterial surface molecules (crude lipopolysaccharide contaminated by peptidoglycans) and it induced morphological changes.

    Keywords
    cell morphology, diptericin, Drosophila, IMD pathway, immune response, proteinase inhibitor
    National Category
    Biological Sciences
    Identifiers
    urn:nbn:se:uu:diva-92571 (URN)10.1111/j.1744-7917.2011.01482.x (DOI)000306786800005 ()
    Available from: 2005-02-11 Created: 2005-02-11 Last updated: 2017-12-14
  • 93.
    Johansson, Karin C
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Metzendorf, Christoph
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Microarray analysis of immune challenged Drosophila hemocytes2005In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 305, no 1, p. 145-155Article in journal (Refereed)
    Abstract [en]

    nsect hemocytes play multiple roles in immunity and carry out cellular responses like phagocytosis, encapsulation and melanization as well as producing humoral effector proteins in the first line of defense after injury and invasion of microorganisms. In this work, we used the Drosophila melanogaster hemocyte-like cell line mbn-2 and Affymetrix Drosophila GeneChips to investigate the transcriptome of a single type of immune competent tissue exposed to Gram-negative cell wall components (crude LPS) or high dose infection with live Escherichia coli. We found that gene expression profiles of both treatments overlap but show important differences in expression levels of several genes involved in immunity. In addition, cell morphology during infection was monitored and revealed distinct alterations in cell shape and adhesion. Presence of large numbers of bacteria also increased the number of cells taking on crystal cell fate. Taken together, our results indicate that hemocytes sense and respond differently to purified bacterial surface molecules and infection with live and actively growing bacteria both at the level of gene expression and in cell behavior.

  • 94.
    Johansson, Karin C
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Cerenius, Lage
    Diptericin expression in bacteria infected Drosophila mbn-2 cells - effect of infection dose and phagocytosisManuscript (Other (popular science, discussion, etc.))
  • 95.
    Johansson, Karin C