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  • 51.
    Feiler, Adam A.
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Stiernstedt, Johanna
    Theander, Katarina
    Jenkins, Paul
    Rutland, Mark W.
    Effect of Capillary Condensation on Friction Force and Adhesion2007In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 23, no 2, p. 517-522Article in journal (Refereed)
    Abstract [en]

    Friction force measurements have been conducted with a colloid probe on mica and silica (both hydrophilic and

    hydrophobized) after long (24 h) exposure to high-humidity air. Adhesion and friction measurements have also been

    performed on cellulose substrates. The long exposure to high humidity led to a large hysteresis between loading and

    unloading in the friction measurements with separation occurring at large negative applied loads. The large hysteresis

    in the friction-load relationship is attributed to a contact area hysteresis of the capillary condensate which built up

    during loading and did not evaporate during the unloading regime. The magnitude of the friction force varied dramatically

    between substrates and was lowest on the mica substrate and highest on the hydrophilic silica substrate, with the

    hydrophobized silica and cellulose being intermediate. The adhesion due to capillary forces on cellulose was small

    compared to that on the other substrates, due to the greater roughness of these surfaces.

  • 52.
    Fornstedt, T
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Sajonz, P
    Guiochon, G
    Thermodynamic study of an unusual chiral separation. Propranolol enantiomers on an immobilized cellulase1997In: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, ISSN 0002-7863, Vol. 119, no 6, p. 1254-1264Article in journal (Other scientific)
    Abstract [en]

    The thermodynamics of interaction of (R)- and (S)-propranolol between an acetic acid buffer (pH = 4.7 and 5.5) and the protein cellobiohydrolase I immobilized on silica gel was studied between 5 and 45 degrees C. The equilibrium data were fitted To a biLa

  • 53.
    Fornstedt, Torgny
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Guiochon, Georges
    Modeling of Chiral liquid chromatography using an Immobilized Protein as the Selector1998In: Quantitative Analysis of Biospecific Interactions; Chapter 6, Amsterdam: OPA, Gordon and Breach Publishing Group, Harwood Academic Publishers , 1998, p. 95-119Chapter in book (Refereed)
  • 54.
    Fornstedt, Torgny
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Guiochon, Georges
    Nonlinear Effects in LC and Chiral LC.2001In: Analytical Chemistry, Vol. 73, p. 608AOther (Other scientific)
  • 55.
    Franco-Fraguas, L.
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Carlsson, J.
    Batista-Viera, F.
    The Adsorption/Desorption Behavior of Horse Radish Peroxidase and Porcine Thyroglobulin on Concanavalin A-Sepharose with Different Ligand Densities2000In: International Journal of Bio-Chromatography, Vol. 5, no 4, p. 255-265Article in journal (Refereed)
  • 56.
    Frazier, A.B.
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Caldwell, K.D.
    Gale, B.K.
    Micromachined Electrical Field-Flow Fractionation System2000Patent (Other (popular scientific, debate etc.))
  • 57.
    Gale, B.K., Caldwell, K.D. and Frazier, A.B.
    Uppsala University, Centre for Surface Biotechnology.
    A Micromachined Electrical Field-Flow Fractionation (m-FFF) System1998In: IEEE Transactions on Biomedical Engineering, Vol. 45, p. 1459-1469Article in journal (Refereed)
  • 58.
    Gale, B.K., Caldwell, K.D. and Frazier, A.B.
    Uppsala University, Centre for Surface Biotechnology.
    Characterization of a Micromachined Electrical Field-Flow Fractionation System1998In: Proc. IEEE 1998 Solid State Sensor and Actuator Workshop, 1998, p. 342-345Conference paper (Other scientific)
  • 59.
    Gale, B.K., Caldwell, K.D., and Frazier, A.B.
    Uppsala University, Centre for Surface Biotechnology.
    Electrical Conductivity Particle Detector for use in Biological and Chemical Micro Analysis Systems1998In: Proc. SPIE Symposium on Microfabrication: Micro Fluidic Devices and Systems, 1998, p. 230-242Conference paper (Other scientific)
  • 60.
    Gale, Bruce K.
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Caldwell, Karin D.
    Frazier, A. Bruno
    Geometric Scaling Effects in Electrical Field Flow Fractionation. 1. Theoretical Analysis2001In: Anal. Chem., Vol. 73, p. 2345-2352Article in journal (Refereed)
  • 61.
    G.-Y. Yan, J.-T. Li, S.-C. Huang and K.D. Caldwell
    Uppsala University, Centre for Surface Biotechnology.
    Calorimetric Observations of Protein Conformation at Solid/Liquid Interfaces1995In: T. Horbett and J. Brash, (eds.), Proteins at Interfaces II,, ACS Press, Washington , 1995, p. 256-268Chapter in book (Other scientific)
  • 62.
    Götmar, Gustav
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
    Albareda, Núria, Robinat
    Fornstedt, Torgny
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Surface Biotechnology. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
    Investigation of the Heterogenous Adsorption Behavior of Selected Enantiomers on Immobilised alpha 1-acid glycoprotein2002In: Analytical Chemistry, Vol. 74, p. 2950-2959Article in journal (Refereed)
  • 63.
    Götmar, Gustav
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Andersson, Marie
    Fornstedt, Torgny
    Guichon, Georges
    Influence of Solute Hydrophobicity on Enantioselective Adsorption of beta-blockers on a Cellulase Protein used as the Chiral Selector.2001In: Journal of Chromatography, Vol. 905, p. 3-17Article in journal (Refereed)
  • 64.
    Götmar, Gustav
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Fornstedt, Torgny
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
    Retention Mechanism of ß-blockers on an immobilized Cellulase. Relative Importance of the Hydrophobic and Ionic Contributions to their Enantioselectivity and Nonselective Interactions2000In: Analytical Chemistry, Vol. 72, p. 3908-3915Article in journal (Refereed)
  • 65.
    Götmar, Gustav
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Fornstedt, Torgny
    Guiochon, Georges
    Apparent and True Enantioselectivity in Enantioseparations2000In: Chirality, Vol. 12, p. 558-564Article in journal (Refereed)
  • 66.
    H. Otsuka, K. Esumi, T.A. Ring, J.-T. Li, and K.D. Caldwell
    Uppsala University, Centre for Surface Biotechnology.
    Simultaneous Adsorption of Polymer and Hydrocarbon/Fluorocarbon Surfactant from their Binary Mixtures on Hydrophilic/Hydrophobic Silica1996In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 116, p. 161-171Article in journal (Refereed)
  • 67. Herron, J. N.
    et al.
    Caldwell, K. D.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
    Wang, H.-K.
    Christensen, D. A.
    Huang, S.-C.
    Janatova, V.
    Apparatus for Multichannel Fluorescent Immunoassay2002Patent (Other (popular scientific, debate etc.))
  • 68.
    Herron, J.N.
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Caldwell, K.D.
    Wang, H.-K.
    Christensen, D.A.
    Huang, S.-C.
    Janatova, V.
    Apparatus and Methods for Multi-Analyte Homogenous Fluoroimmunoassays1999Patent (Other (popular scientific, debate etc.))
  • 69.
    Herron, J.N.
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Christensen, D.A.
    Caldwell, K.D.
    Huang, S.C.
    Wang, H.K.
    Apparatus and Methods for Multi-analyte Homogenous Fluoro-immunoassays1997Patent (Other (popular scientific, debate etc.))
  • 70.
    Herron, J.N.
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Christensen, D.A.
    Caldwell, K.D.
    Janatova, V.
    Huang, S.C.
    Wang, H.K.
    Waveguide Immunosensor with Coating Chemistry Providing Enhanced Sensitivity1998Patent (Other (popular scientific, debate etc.))
  • 71.
    Herron, J.N.
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Christensen, D.A.
    Caldwell, K.D.
    Wang, H.K.
    Huang, S.C.
    Janatova, V.
    Waveguide Immunosensor with Coating Chemistry Providing Enhanced Sensitivity1996Patent (Other (popular scientific, debate etc.))
  • 72.
    Herron, J.N., Durtschi, J.D., Wang, H-K, Janatova, V., Chang, I-N, Huang, S-C, Caldwell, K.D., and Christensen, D.A.
    Uppsala University, Centre for Surface Biotechnology.
    Orientation and Activity of Immobilized Antibodies1998In: M. Malmsten, (ed.), Biopolymers at Interfaces, Dekker, NY , 1998, Vol. 75, p. 221-280Chapter in book (Other scientific)
  • 73.
    Hjort, Rolf
    Uppsala University, Centre for Surface Biotechnology.
    A study of some operational parameters in expanded bed adsorption of proteins2000Licentiate thesis, monograph (Other scientific)
  • 74.
    Hjorth, R.
    Uppsala University, Centre for Surface Biotechnology.
    Expanded bed adsorption: elution in expanded bed mode1999In: Bioseparation, Vol. 8, p. 1-Article in journal (Refereed)
  • 75.
    Hjorth, R.
    Uppsala University, Centre for Surface Biotechnology.
    Expanded-bed adsorption in industrial bioprocessing: recent developments1997In: Trends in biotechnology, Vol. 15, p. 230-235Article in journal (Refereed)
  • 76.
    Hjorth, R., Kämpe, S., and Carlsson, M.
    Uppsala University, Centre for Surface Biotechnology.
    Analysis of some operating parameters of novel adsorbents for recovery of proteins in expanded beds1995In: Bioseparation, Vol. 5, p. 217-223Article in journal (Refereed)
  • 77.
    Hjorth, R., Leijon, P., Barnfield-Frej, A.-K., and Jägersten, C.
    Uppsala University, Centre for Surface Biotechnology.
    Expanded bed adsorption chromatography1998In: G. Subramanian, (ed.), Bioseparation and Bioprocessing, Wiley-VCH, Weinheim , 1998, p. 199-226Chapter in book (Other scientific)
  • 78.
    Ho, C.-H., Limberis, L., Caldwell, K.D., and Stewart, R.J.
    Uppsala University, Centre for Surface Biotechnology.
    A Metal-Chelating Pluronic for Immobilization of Histidine-Tagged Proteins at Interfaces: Immobilization of Firefly Luciferase on Polystyrene Beads1998In: Langmuir, Vol. 14, p. 3889-3894Article in journal (Refereed)
  • 79.
    Huang, Shao-Chie
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Tresco, Patrick A.
    Caldwell, Karin D.
    Structural integrity in protein immobilization.1997In: Polymer Preprints, The Division of PolymerChemistry, Inc.; American Chemical Society , 1997, Vol. 38, no 1, p. 561-562Conference paper (Other scientific)
  • 80.
    J. Carlsson, J.-C. Janson and M. Sparrman
    Uppsala University, Centre for Surface Biotechnology.
    Affinity Chromatography1998In: J.-C. Janson and L. Rydén, (eds.), Protein Purification, Principles, High Resolution Methods and Applications, John Wiley & Sons Inc., New York , 1998, p. 375-443Chapter in book (Other scientific)
  • 81.
    Janne, K
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Pettersen, J
    Lindberg, NO
    Lundstedt, T
    Medicinska vetenskapsområdet, Faculty of Pharmacy, Department of Medicinal Chemistry.
    Hierarchical principal component analysis (PCA) and projection to latent structure (PLS) technique on spectroscopic data as a data pretreatment for calibration2001In: JOURNAL OF CHEMOMETRICS, ISSN 0886-9383, Vol. 15, no 4, p. 203-213Article in journal (Refereed)
    Abstract [en]

    Spectroscopic data consists of several hundred to some thousand variables, wherein most of the variables are: autocorrelated. When PCA and PLS techniques are used for the interpretation of these kinds of data, the loading plots are usually complex due to

  • 82.
    Janson, Jan-Christer
    Uppsala University, Centre for Surface Biotechnology.
    Optimization of large-scale chromatography of proteins2001In: KOREAN JOURNAL OF CHEMICAL ENGINEERING, ISSN 0256-1115, Vol. 18, no 2, p. 149-158Article in journal (Refereed)
    Abstract [en]

    Protein chromatography is a very complex process based on a combination of thermodynamic, kinetic and mass transport phenomena. By virtue of their complicated and delicate surface structures, the behaviour of proteins on various chromatographic media is n

  • 83.
    Janson, Jan-Christer
    Uppsala University, Centre for Surface Biotechnology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Physical and Analytical Chemistry.
    The Development of Gel Media and Columns for Large-Scale Chromatography of Proteins, a Historical Review2002In: Chinese J. Chem. Eng, Vol. 10, no 6, p. 690-695Article in journal (Refereed)
  • 84.
    J.-C., Janson and J.-Å. Jönsson
    Uppsala University, Centre for Surface Biotechnology.
    Introduction to Chromatography1998In: J.-C. Janson and L. Rydén, (eds.), Protein Purification, Principles, High Resolution Methods and Applications, John Wiley & Sons Inc., New York , 1998, p. 43-78Chapter in book (Other scientific)
  • 85.
    Joerger, R
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Materials Science. SOLID STATE PHYSICS.
    Klaus, T
    Centre for Surface Biotechnology.
    Granqvist, C.G.
    Functional Biomimetic Surface Coatings.2000In: The Annals of MCFA, Vol. 1, no 1, p. 39-44Article in journal (Refereed)
    Abstract [en]

    An interdisciplinary approach to materials science is proposed, involving biological methods that complement existing physical and chemical coating techniques. Silver-dielectric composite thin films were produced by a bio-logical technique, using microbia

  • 86.
    Joerger, R
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Materials Science. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Materials Science. SOLID STATE PHYSICS.
    Klaus, T
    Centre for Surface Biotechnology.
    Pettersson, J
    Chemistry, Department of Chemistry.
    Granqvist, C G
    Digestion method for silver accumulated in micro-organisms2000In: Fresenius J. Anal.Chem, Vol. 366, no 3, p. 311-312Article in journal (Refereed)
    Abstract [en]

    Silver is accumulated to high concentrations in certain microbial strains. Here a bomb digestion method is proposed, using HNO3 and HCl, for the extraction and digestion of silver and silver compounds from the organic matrix. The method is applicable for

  • 87.
    J.-T., Li and K.D. Caldwell
    Uppsala University, Centre for Surface Biotechnology.
    Plasma Protein Interactions with Pluronic™ Treated Colloids1996In: Colloids and Surfaces B: Biointerfaces, Vol. 7, p. 009-022Article in journal (Refereed)
  • 88.
    J.-T. Li, J. Carlsson, J.N. Lin, and K. D. Caldwell
    Uppsala University, Centre for Surface Biotechnology.
    Chemical Modification of Surface Active Poly(Ethylene Oxide)-Poly(Propylene Oxide) Triblock Copolymers1996In: Bioconjugate Chemistry, Vol. 7, p. 592-599Article in journal (Refereed)
  • 89.
    J.-T. Li, J. Carlsson, S.-C. Huang and K.D. Caldwell
    Uppsala University, Centre for Surface Biotechnology.
    Adsorption of Poly(ethylene oxide)-Containing Block Copolymers: A Route to Protein Resistance1996In: E. Glass, Ed., Hydrophilic Polymers, ACS Press, Washington , 1996, p. 61-78Chapter in book (Other scientific)
  • 90.
    Karlsson, Marjam
    Uppsala University, Centre for Surface Biotechnology.
    Nano-pourus alumina, a potential bone implant coating (Mechanical properties and in vitro cell culture studies)2002Licentiate thesis, monograph (Other scientific)
  • 91.
    Karlsson, Marjam
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Surface Biotechnology.
    Pålsgård, Eva
    Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
    Wilshaw, Peter R
    Di Silvio, Lucy
    Initial in vitro interaction of osteoblasts with nano-porous alumina2003In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 24, no 18, p. 3039-3046Article in journal (Refereed)
    Abstract [en]

    In the present study we have used a characterised primary human cell culture model to investigate cellular interactions with nano-porous alumina. This material, prepared by anodisation, is being developed as a coating on titanium alloy implants. The structure of the alumina, as determined by X-ray diffraction and transmission electron microscopy, was amorphous. When studying cell/material interactions we used both biochemical and morphological parameters. Cell viability, proliferation and phenotype were assessed by measurement of redox reactions in the cells, cellular DNA, tritiated thymidine ([H-3]-TdR) incorporation and alkaline phosphatase (ALP) production. Results showed a normal osteoblastic growth pattern with increasing cell numbers during the first 2 weeks. A peak in cell proliferation was seen on day 3, after which cell growth decreased, followed by an increase in ALP production, thus indicating that the osteoblastic phenotype was retained on the alumina. Cell adhesion was observed, the osteoblast-like cells having a flattened morphology with filipodia attached to the pores of the material. SDS-PAGE and western blot measurements showed that the nano-porous alumina was able to adsorb fibronectin. Trace amounts of aluminium ions were measured in the surrounding medium, but no adverse effect on cell activity was observed.

  • 92.
    Klaus, T
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Materials Science. Centre for Surface Biotechnology. SOLID STATE PHYSICS.
    Joerger, R
    Olsson, E
    Granqvist, CG
    Silver-based crystalline nanoparticles, microbially fabricated1999In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, ISSN 0027-8424, Vol. 96, no 24, p. 13611-13614Article in journal (Refereed)
    Abstract [en]

    One mechanism of silver resistance in microorganisms is accumulation of the metal ions in the cell. Here, we report on the phenomenon of biosynthesis of silver-based single crystals with well-defined compositions and shapes, such as equilateral trian

  • 93.
    Larsericsdotter, H
    et al.
    Uppsala University, Centre for Surface Biotechnology. IONPHYSICS.
    Oscarsson, S
    Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Materials Science.
    Buijs, J
    Thermodynamic analysis of proteins adsorbed on silica particles: Electrostatic effects2001In: JOURNAL OF COLLOID AND INTERFACE SCIENCE, ISSN 0021-9797, Vol. 237, no 1, p. 98-103Article in journal (Refereed)
    Abstract [en]

    Electrostatic effects on protein adsorption were investigated using differential scanning calorimetry (DSC) and adsorption isotherms, The thermal denaturation of lysozyme, ribonuclease A (RNase), and alpha -lactalbumin in solution and adsorbed onto silica

  • 94.
    Larsericsdotter, Helén
    Uppsala University, Centre for Surface Biotechnology.
    Macromolecules at Interfaces2004Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    In this thesis, the structure and stability of globular proteins adsorbed onto nanometer-sized hydrophilic silica particles were investigated using differential scanning calorimetry (DSC), hydrogen/deuterium exchange (HDX), and mass spectrometry (MS). The adsorption process itself was characterized with fluorescence and absorption spectroscopy and surface plasmon resonance (SPR). The combination of these methods offered a unique insight into adsorption-induced changes within proteins related to their adsorption characteristics. DSC contributed with thermodynamic information on the overall structural stability within the protein population. HDX in combination with MS contributed information on the structure and stability of adsorbed proteins with focus on changes within the secondary structure elements. In order to increase the structural resolution in this part of the investigation, proteolysis was performed prior to the MS analyzing step. Knowledge on the protein adsorption process was utilized in a practical approach called ligand fishing. In this approach, SPR was used to monitor the chip-based affinity purification of a protein with MS used for protein identification.

    Adsorption isotherms revealed that electrostatic interactions play an important role in the adsorption of proteins to hydrophilic surfaces. DSC investigation revealed that the thermal stability of proteins reduces with increasing electrostatic attraction between the protein and the surface and that this effect diminishes at higher surface coverage. The mass-increase due to exchange between protein hydrogen atoms and deuterium atoms in solution was investigated as a function of time. This gave insight into adsorption-induced changes in the structural stability of proteins. By combining DSC and HDX-MS, it was possible to differentiate between adsorption-induced changes in the secondary and tertiary structure. Additionally, if limited proteolysis was performed, the investigations gave insight into the orientation and protein segment specific changes in the stability of proteins adsorbed to silica surfaces. The adsorption of proteins to silica particles also provided the basis for a new experimental design that allows handling of minute amounts of proteins in a ligand fishing application, as used in the field of functional proteomics.

    List of papers
    1. Thermodynamic Analysis of Proteins Adsorbed on Silica Particles: Electrostatic Effects
    Open this publication in new window or tab >>Thermodynamic Analysis of Proteins Adsorbed on Silica Particles: Electrostatic Effects
    2001 In: Journal of Colloid and Interface Science, Vol. 237, no 1, p. 98-103Article in journal (Refereed) Published
    Identifiers
    urn:nbn:se:uu:diva-92363 (URN)
    Available from: 2004-11-17 Created: 2004-11-17Bibliographically approved
    2. Localized changes in the structural stability of myoglobin upon adsorption onto silica particles, as studied with hydrogen/deuterium exchange mass spectrometry
    Open this publication in new window or tab >>Localized changes in the structural stability of myoglobin upon adsorption onto silica particles, as studied with hydrogen/deuterium exchange mass spectrometry
    Show others...
    2003 (English)In: Journal of Colloid and Interface Science, ISSN 0021-9797, Vol. 263, no 2, p. 441-448Article in journal (Refereed) Published
    National Category
    Natural Sciences
    Identifiers
    urn:nbn:se:uu:diva-92364 (URN)10.1016/S0021-9797(03)00401-6 (DOI)
    Available from: 2004-11-17 Created: 2004-11-17 Last updated: 2012-04-13Bibliographically approved
    3. Thermodynamic analysis of lysozyme adsorbed to silica
    Open this publication in new window or tab >>Thermodynamic analysis of lysozyme adsorbed to silica
    2004 In: Journal of Colloid and Interface Science, Vol. 276, no 2, p. 261-268Article in journal (Refereed) Published
    Identifiers
    urn:nbn:se:uu:diva-92365 (URN)
    Available from: 2004-11-17 Created: 2004-11-17Bibliographically approved
    4. Structure, Stability, and Orientation of BSA Adsorbed to Silica
    Open this publication in new window or tab >>Structure, Stability, and Orientation of BSA Adsorbed to Silica
    In: Journal of Colloid and Interface ScienceArticle in journal (Refereed) Submitted
    Identifiers
    urn:nbn:se:uu:diva-92366 (URN)
    Available from: 2004-11-17 Created: 2004-11-17Bibliographically approved
    5. Combining Surface Plasmon Resonance and Mass Spectrometry in Functional Proteomics: How to avoid and utilize non-specific adsorption
    Open this publication in new window or tab >>Combining Surface Plasmon Resonance and Mass Spectrometry in Functional Proteomics: How to avoid and utilize non-specific adsorption
    Show others...
    In: ProteomicsArticle in journal (Refereed) Submitted
    Identifiers
    urn:nbn:se:uu:diva-92367 (URN)
    Available from: 2004-11-17 Created: 2004-11-17Bibliographically approved
  • 95.
    Larsericsdotter, Helén
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Jansson, Östen
    Zhukov, Andrei
    Areskoug, Daphne
    Oscarsson, Sven
    Larsericsdotter, Helén
    Uppsala University, Centre for Surface Biotechnology.
    Combining Surface Plasmon Resonance and Mass Spectrometry in Functional Proteomics: How to avoid and utilize non-specific adsorptionIn: ProteomicsArticle in journal (Refereed)
  • 96.
    Larsericsdotter, Helén
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Oscarsson, Sven
    Buijs, Jos
    Structure, Stability, and Orientation of BSA Adsorbed to SilicaIn: Journal of Colloid and Interface ScienceArticle in journal (Refereed)
  • 97.
    Larsericsdotter, Helén
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Oscarsson, Sven
    Buijs, Jos
    Thermodynamic analysis of lysozyme adsorbed to silica2004In: Journal of Colloid and Interface Science, Vol. 276, no 2, p. 261-268Article in journal (Refereed)
  • 98.
    Larsericsdotter, Helén
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Oscarsson, Sven
    Buijs, Jos
    Thermodynamic Analysis of Proteins Adsorbed on Silica Particles: Electrostatic Effects2001In: Journal of Colloid and Interface Science, Vol. 237, no 1, p. 98-103Article in journal (Refereed)
  • 99.
    Ledung, G.
    et al.
    Uppsala University, Centre for Surface Biotechnology.
    Bergkvist, M.Quist, A.Gelius, U.Oscarsson, S.
    A Novel Method for Preparation of Highly reactive Disulphides on Silicon2001In: Proceedings 8th International Conference on Composite Engineering August 5-11, 2001, Tenerife, SpainConference proceedings (editor) (Other scientific)
  • 100.
    Lindberg, J. and Oscarsson, S
    Uppsala University, Centre for Surface Biotechnology.
    Surface Plasmon Resonance Technique for Studies of Protein Adsorption on Biomaterials1996In: 3: rd Meeting and Seminar on : Ceramics,Cells and Tissues, (Ed. Ravaglioli,A. and Krajewski,A.). Gruppo editoriale faenza editrice s.p.a., 1996, p. 171-178Conference paper (Other scientific)
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