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  • 9301.
    Zhang, Xiaoliang
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Hägglund, Carl
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets elektronik.
    Johansson, Malin B
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Sveinbjörnsson, Kari
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Johansson, Erik M
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Fine Tuned Nanolayered Metal/Metal Oxide Electrode for Semitransparent Colloidal Quantum Dot Solar Cells2016Ingår i: Advanced Functional Materials, ISSN 1616-301X, E-ISSN 1616-3028, Vol. 26, nr 12, s. 1921-1929Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Semitransparent photovoltaics have great potential, for example, in buildingintegrationor in portable electronics. However, the front and back contactelectrodes signifi cantly affect the light transmission and photovoltaic performanceof the complete device. Herein, the use of a semitransparentnanolayered metal/metal oxide electrode for a semitransparent PbS colloidalquantum dot solar cell to increase the light transmission and power conversioneffi ciency is reported. The effect of the nanolayered electrode on theoptical properties within the solar cells is studied and compared to a theoreticallymodel to identify the origin of optical losses that lower the devicetransmission. The results show that the light transmission in the visibleregion and the photovoltaic performance are signifi cantly enhanced with thenanolayered electrode. The solar cell shows an effi ciency of 5.4% and averagevisible transmittance of 24.1%, which is an increase by 28.6% and 59.6%,respectively, compared to the device with a standard Au fi lm as the electrode.These results demonstrate that the optical and electrical modifi cation oftransparent electrode is possible and essential for reducing the light refl ectionand absorption of the electrode in semitransparent photovoltaics, and,meanwhile the demonstrated nanolayered materials may provide an avenuefor enhancing the device transparency and efficiency.

  • 9302.
    Zhang, Xiaoliang
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Hägglund, Carl
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets elektronik.
    Johansson, Malin B
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Sveinbjörnsson, Kári
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Liu, Jianhua
    Beihang Univ, Sch Mat Sci & Engn, Beijing 100191, Peoples R China..
    Johansson, Erik
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    FTO-free top-illuminated colloidal quantum dot electro-optics in devices2017Ingår i: Solar Energy, ISSN 0038-092X, E-ISSN 1471-1257, Vol. 158, s. 533-542Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A solar cell device architecture with top-illumination, where the light does not pass through the substrate, is advantageous for many applications. It is also specifically useful for the construction of tandem or multiple junction photovoltaic devices, with illumination through the top solar cell. Here, a top-illuminated colloidal quantum dot solar cell (TI-CQDSC) is demonstrated and compared with a conventional colloidal quantum dot solar cell (C-CQDSC) constructed on a FTO (fluorine doped tin oxide) glass substrate both theoretically and experimentally. The optical electric field distribution in the solar cells with different configuration is simulated using transfer matrix formalism and a more intense optical electric field was observed in TI-CQDSC, leading to a higher exciton generation rate within the colloidal quantum dot solid. The TI-CQDSCs are constructed on both nonconductive glass and flexible substrates, and a maximum power conversion efficiency of 6.4% and 5.6% is achieved, respectively, comparing to that of 5.9% for the C-CQDSC. The improved performance of the top illuminated solar cell is attributed to a combination of enhanced optical electric field intensity in the colloidal quantum dot solid and superior conductivity of the transparent metal film electrode.

  • 9303.
    Zhang, Xiaoliang
    et al.
    Beihang Univ, Sch Mat Sci & Engn, Beijing 100191, Peoples R China.
    Jia, Donglin
    Beihang Univ, Sch Mat Sci & Engn, Beijing 100191, Peoples R China.
    Hägglund, Carl
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets elektronik.
    Öberg, Viktor A.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Du, Juan
    Beihang Univ, Sch Mat Sci & Engn, Beijing 100191, Peoples R China.
    Liu, Jianhua
    Beihang Univ, Sch Mat Sci & Engn, Beijing 100191, Peoples R China.
    Johansson, Erik M. J.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Highly photostable and efficient semitransparent quantum dot solar cells by using solution-phase ligand exchange2018Ingår i: Nano Energy, ISSN 2211-2855, E-ISSN 2211-3282, Vol. 53, s. 373-382Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    For semitransparent solar cells (SSCs) the photovoltaic efficiency and the transparency are the two primary objectives for utilization in for example building integrated photovoltaics. Solution-processed PbS colloidal quantum dot (CQD) has strong light absorption in the ultraviolent region and possess the advantages of tunable bandgap in the visible and infrared region. Herein we report a PbS CQD-SSC with tunable infrared light absorption and high photostability by combining experimental studies and numerical theoretical simulations. Through fine-controlling the electro-optics in the CQD-SSC and by using a solution-phase ligand exchange for the CQD solid film deposition, the power loss in the device is significantly decreased, yielding a CQD-SSC with a power conversion efficiency of 8.4% and an average visible transmittance of 21.4%, respectively. After 540 h continuous 100 mW cm(-2) illumination the solar cell still shows similar to 85% of its initial power conversion efficiency, and then recovers to the initial performance after storage in dark. This work provides a strong progress and an approach toward the development of low-cost, highly efficient and stable semitransparent CQD solar cells. Meanwhile this study also provides insight and quantitative guidelines for further improving the SSC photovoltaic efficiency and transparency in general.

  • 9304.
    Zhang, Xiaoliang
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Johansson, Erik M. J.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Reduction of charge recombination in PbS colloidal quantum dot solar cells at the quantum dot/ZnO interface by inserting a MgZnO buffer layer2017Ingår i: Journal of Materials Chemistry A, ISSN 2050-7488, Vol. 5, nr 1, s. 303-310Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Interfacial charge recombination occurring at the interface between a colloidal quantum dot (CQD) solid and the electron collecting layer (ECL) in CQD solar cells significantly affects the charge carrier collection, therefore limiting the device photovoltaic performance. In this work CQD solar cells with an improved performance are reported by employing MgZnO as a buffer layer (BL) with tunable electronic energy levels in the solar cells to reduce interfacial charge recombination and hence improve the solar cell photovoltaic performance. The effect of the BL on the solar cell performance is experimentally investigated and compared to theoretical calculations. Incorporation of a BL with favorable electronic energy levels forming a suitable band alignment with the CQD layer in solar cells diminishes the interfacial charge recombination and an increased photovoltage can be obtained. A CQD solar cell with a BL shows a power conversion efficiency of up to 9.3%, compared to that of 8.2% for a solar cell without any BLs. The unsealed solar cells are also rather stable under ambient conditions both in the dark and under continuous illumination. This work suggests that a MgZnO BL with energy level tunability provides a potential strategy to improve the interfacial properties of CQD photovoltaic devices.

  • 9305.
    Zhang, Xiaoliang
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Johansson, Erik M. J.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Utilizing light trapping interference effects in microcavity structured colloidal quantum dot solar cells: A combined theoretical and experimental approach2016Ingår i: NANO ENERGY, ISSN 2211-2855, Vol. 28, s. 71-77Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Colloidal quantum dot (CQD) solar cells are promising devices for solar energy conversion. However, usually the CQD layer in the devices needs to be thin to avoid recombination of the photogenerated charges before reaching the contacts. A microcavity structure with metal layers on both sides on the CQD film gives light interference effects in the CQD layer, which may increase the light absorption in the solar cell. However, such interference effects are not well known for CQD films, and differences compared to solid materials may appear. In this work the interference effects in CQD solar cells with different thicknesses of the CQD layer is investigated and the results are compared to simulations of the electrooptical field in the devices. The results show that the interference effects can be used to enhance the efficiency of the devices and the interference in the microcavity structure is favorable for efficient photocurrent generation also in thin CQD layers. Using the results, solar cells based on thin CQD layers on flexible or glass substrates are prepared and the results are very promising.

  • 9306.
    Zhang, Xiaoliang
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Justo, Yolanda
    Maes, Jorick
    Walravens, Willem
    Zhang, Jindan
    Liu, Jianhua
    Hens, Zeger
    Johansson, Erik M. J.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Slow recombination in quantum dot solid solar cellusing p–i–n architecture with organic p-type holetransport material2015Ingår i: Journal of Materials Chemistry A, ISSN 2050-7488, Vol. 3, nr 41, s. 20579-20585Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The interfaces between different materials in the heterojunction colloidal quantum dot (QD) solar cell playan important role for charge carrier separation, recombination and collection. Here, an organic–inorganichybrid p–i–n architecture for the heterojunction PbS QD solid solar cell is constructed to increase thecharge extraction and reduce charge recombination. Heavily doped poly(3-hexylthiophene-2,5-diyl)(P3HT) is applied as hole transport interlayer between the QD film and metal contact electrode. Theresults show that the P3HT interlayer diminishes the charge carrier recombination at the QD film/metalcontact electrode interface leading to increased open-circuit voltage and increased electron life time.Furthermore, after incorporation of P3HT interlayer an additional p–i heterojunction might form atP3HT/QD film interface resulting in increased depletion region, which promotes charge carrierextraction under working conditions. Two other organic p-type interlayers are also investigated,however, the results indicate that a barrier for charge extraction is formed for these devices, which isexplained by the difference in energy levels. The solar cell with the P3HT interlayer exhibits a powerconversion efficiency of 5.1% at 1 sun of illumination and ambient atmosphere, which is 20% highercompared to the solar cell without any hole transport interlayer.

  • 9307.
    Zhang, Xiaoliang
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Liu, Jianhua
    Johansson, Erik M. J.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Efficient charge-carrier extraction from Ag2Squantum dots prepared by the SILAR method for utilization of multiple exciton generation2015Ingår i: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 7, nr 4, s. 1454-1462Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The utilization of electron–hole pairs (EHPs) generated from multiple excitons in quantum dots (QDs) is of great interest toward efficient photovoltaic devices and other optoelectronic devices; however, extraction of charge carriers remains difficult. Herein, we extract photocharges from Ag2S QDs and investigate the dependence of the electric field on the extraction of charges from multiple exciton generation (MEG). Low toxic Ag2S QDs are directly grown on TiO2 mesoporous substrates by employing the successive ionic layer adsorption and reaction (SILAR) method. The contact between QDs is important for the initial charge separation after MEG and for the carrier transport, and the space between neighbor QDs decreases with more SILAR cycles, resulting in better charge extraction. At the optimal electric field for extraction of photocharges, the results suggest that the threshold energy (th) for MEG is 2.41Eg. The results reveal that Ag2S QD is a promising material for efficient extraction of charges from MEG and that QDs prepared by SILAR have an advantageous electrical contact facilitating charge separation and extraction.

  • 9308.
    Zhang, Xiaoliang
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Liu, Jianhua
    Zhang, Jindan
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Vlachopoulos, Nick
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Johansson, Erik M. J.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    ZnO@Ag2S core-shell nanowire arrays for environmentally friendly solid-state quantum dot-sensitized solar cells with panchromatic light capture and enhanced electron collection2015Ingår i: Physical Chemistry, Chemical Physics - PCCP, ISSN 1463-9076, E-ISSN 1463-9084, Vol. 17, nr 19, s. 12786-12795Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A solid-state environmentally friendly Ag2S quantum dot-sensitized solar cell (QDSSC) is demonstrated. The photovoltaic device is fabricated by applying ZnO@Ag2S core-shell nanowire arrays (NWAs) as light absorbers and electron conductors, and poly-3-hexylthiophene (P3HT) as a solid-state hole conductor. Ag2S quantum dots (QDs) were directly grown on the ZnO nanowires by the successive ionic layer adsorption and reaction (SILAR) method to obtain the core-shell nanostructure. The number of SILAR cycles for QD formation and the length of the core-shell NWs significantly affect the photocurrent. The device with a core-shell NWAs photoanode shows a power conversion efficiency increase by 32% compared with the device based on a typical nanoparticle-based photoanode with Ag2S QDs. The enhanced performance is attributed to enhanced collection of the photogenerated electrons utilizing the ZnO nanowire as an efficient pathway for transporting the photogenerated electrons from the QD to the contact.

  • 9309.
    Zhang, Xiaoliang
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Santra, Pralay Kanti
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Molekyl- och kondenserade materiens fysik.
    Tian, Lei
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Johansson, Malin B.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Rensmo, Håkan
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Molekyl- och kondenserade materiens fysik.
    Johansson, Erik M.J.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Highly Efficient Flexible Quantum Dot Solar Cells with Improved Electron Extraction Using MgZnO Nanocrystals2017Ingår i: ACS Nano, ISSN 1936-0851, E-ISSN 1936-086X, Vol. 11, nr 8, s. 8478-8487Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Colloidal quantum dot (CQD) solar cells have high potential for realizing an efficient and lightweight energy supply for flexible or wearable electronic devices. To achieve highly efficient and flexible CQD solar cells, the electron transport layer (ETL), extracting electrons from the CQD solid layer, needs to be processed at a low-temperature and should also suppress interfacial recombination. Herein, a highly stable MgZnO nanocrystal (MZO-NC) layer is reported for efficient flexible PbS CQD solar cells. Solar cells fabricated with MZONC ETL give a high power conversion efficiency (PCE) of 10.4% and 9.4%, on glass and flexible plastic substrates, respectively. The reported flexible CQD solar cell has the record efficiency to date of flexible CQD solar cells. Detailed theoretical simulations and extensive characterizations reveal that the MZO-NCs significantly enhance charge extraction from CQD solids and diminish the charge accumulation at the ETL/CQD interface, suppressing charge interfacial recombination. These important results suggest that the low-temperature processed MZO-NCs are very promising for use in efficient flexible solar cells or other flexible optoelectronic devices.

  • 9310.
    Zhang, Xiaoliang
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Welch, Ken
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Nanoteknologi och funktionella material.
    Tian, Lei
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Johansson, Malin B
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Häggman, Leif
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Liu, Jianhua
    Beihang Univ, Sch Mat Sci & Engn, Beijing 100191, Peoples R China.
    Johansson, Erik MJ
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Enhanced charge carrier extraction by a highly ordered wrinkled MgZnO thin film for colloidal quantum dot solar cells2017Ingår i: Journal of Materials Chemistry C, ISSN 2050-7526, E-ISSN 2050-7534, Vol. 5, nr 42, s. 11111-11120Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Efficient charge carrier extraction from a colloidal quantum dot (CQD) solid is crucial for highperformance of CQD solar cells (CQDSCs). Herein, highly ordered wrinkled MgZnO (MZO) thin films aredemonstrated to improve the charge carrier extraction of PbS CQDSCs. The highly ordered wrinkledMZO thin films are prepared using a low-temperature combustion method. The photovoltaicperformances of CQDSCs with a combustion-processed MZO (CP-MZO) thin film as an electrontransport material (ETM) are compared to those of CQDSCs with a conventional sol–gel processed MZO(SGP-MZO) thin film as an ETM. We performed photoluminescence quenching measurements of thecolloidal quantum dot (CQD) solid and charge carrier dynamic analysis of full solar cell devices. Theresults show that the highly ordered wrinkled CP-MZO thin film significantly increases the chargecarrier extraction from the CQD solid and therefore diminishes the charge interfacial recombination atthe CQD/ETM junction, leading to a 15.5% increase in power conversion efficiency. The improvedefficiency in the CP-MZO based CQDSC is also attributed to the compact and pin-hole free CP-MZOthin film.

  • 9311.
    Zhang, Xiaoliang
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Zhang, Jindan
    Liu, Jianhua
    Johansson, Erik M. J.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Solution processed flexible and bending durable heterojunction colloidal quantum dot solar cell2015Ingår i: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 7, nr 27, s. 11520-11524Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A flexible hybrid heterojunction PbS colloidal quantum dot solar cell, where the photoactive layers are deposited using a solution processed fabrication approach under ambient condition and at room temperature is presented. The bending stability of the obtained solar cell is evaluated. The results show that the solar cell exhibits high bending stability and even under the bent state the cell also maintains a high performance, which shows the potential of the quantum dot solar cell toward a lightweight, bendable power source with many possible applications.

  • 9312.
    Zhang, Xiaoliang
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Zhang, Jindan
    Beihang Univ, Sch Mat Sci & Engn, Beijing 100191, Peoples R China..
    Phuyal, Dibya
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Molekyl- och kondenserade materiens fysik.
    Du, Juan
    Beihang Univ, Sch Mat Sci & Engn, Beijing 100191, Peoples R China..
    Tian, Lei
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Öberg, Viktor A.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Johansson, Malin B
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Cappel, Ute B.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Molekyl- och kondenserade materiens fysik.
    Karis, Olof
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Molekyl- och kondenserade materiens fysik. Uppsala Univ, Mol & Condensed Matter Phys, Dept Phys & Astron, S-75120 Uppsala, Sweden..
    Liu, Jianhua
    Beihang Univ, Sch Mat Sci & Engn, Beijing 100191, Peoples R China..
    Rensmo, Håkan
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Molekyl- och kondenserade materiens fysik.
    Boschloo, Gerrit
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Johansson, Erik M. J.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Inorganic CsPbI3 Perovskite Coating on PbS Quantum Dot for Highly Efficient and Stable Infrared Light Converting Solar Cells2018Ingår i: Advanced Energy Materials, ISSN 1614-6832, Vol. 8, nr 6, artikel-id 1702049Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Solution-processed colloidal quantum dot (CQD) solar cells harvesting the infrared part of the solar spectrum are especially interesting for future use in semitransparent windows or multilayer solar cells. To improve the device power conversion efficiency (PCE) and stability of the solar cells, surface passivation of the quantum dots is vital in the research of CQD solar cells. Herein, inorganic CsPbI3 perovskite (CsPbI3-P) coating on PbS CQDs with a low-temperature, solution-processed approach is reported. The PbS CQD solar cell with CsPbI3-P coating gives a high PCE of 10.5% and exhibits remarkable stability both under long-term constant illumination and storage under ambient conditions. Detailed characterization and analysis reveal improved passivation of the PbS CQDs with the CsPbI3-P coating, and the results suggest that the lattice coherence between CsPbI3-P and PbS results in epitaxial induced growth of the CsPbI3-P coating. The improved passivation significantly diminishes the sub-bandgap trap-state assisted recombination, leading to improved charge collection and therefore higher photovoltaic performance. This work therefore provides important insight to improve the CQD passivation by coating with an inorganic perovskite ligand for photovoltaics or other optoelectronic applications.

  • 9313.
    Zhang, Xiaoliang
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi. Beihang Univ, Sch Mat Sci & Engn, Beijing 100191, Peoples R China.
    Öberg, Viktor A.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Du, Juan
    Beihang Univ, Sch Mat Sci & Engn, Beijing 100191, Peoples R China..
    Liua, Jianhua
    Beihang Univ, Sch Mat Sci & Engn, Beijing 100191, Peoples R China..
    Johansson, Erik
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Extremely lightweight and ultra-flexible infrared light-converting quantum dot solar cells with high power-per-weight output using a solution-processed bending durable silver nanowire-based electrode2018Ingår i: Energy & Environmental Science, ISSN 1754-5692, E-ISSN 1754-5706, Vol. 11, nr 2, s. 354-364Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Lightweight and flexible solar cells are highly interesting materials for use in new applications, such as spacecraft, aircraft and personal pack load. PbS colloidal quantum dots (CQDs) exhibit a broad and strong light absorption spectrum covering the ultraviolet-visible-near infrared region, allowing for incorporation of very thin CQD films into solar cells with high power conversion efficiency (PCE) from solar light to electricity. Herein, we report an extremely lightweight and ultra-flexible CQD solar cell constructed on a polyethylene naphthalate substrate with a thickness of 1.3 mu m. A solution-processed Ag nanowire network with excellent mechanical, optical and electrical properties was prepared as the front-electrode in the solar cell. The thickness of the complete CQD solar cell is less than 2 mm, and similar to 10% PCE with a weight of 6.5 g m(-2) is achieved, resulting in a power-per-weight output of 15.2 W g(-1). The flexible solar cell possesses durable mechanical properties and maintains high-level photovoltaic performance under extreme deformation and after repeated compression-stretching deformation. Moreover, the flexible CQD solar cell shows impressive stability both under continuous illumination and after storage under ambient conditions. These results reveal that solution-processed CQDs are compatible with an ultra-flexible substrate for the construction of ultra-lightweight infrared light-converting CQD solar cells with possibilities for new exciting solar energy applications.

  • 9314. Zhang, Xiaoou
    et al.
    Samuelsson, Jörgen
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för fysikalisk och analytisk kemi.
    Janson, Jan-Christer
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för fysikalisk och analytisk kemi.
    Wang, Changhai
    Su, Zhiguo
    Gu, Ming
    Fornstedt, Torgny
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för fysikalisk och analytisk kemi.
    Investigation of the adsorption behavior of glycine peptides on 12% cross-linked agarose gel media2010Ingår i: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1217, nr 12, s. 1916-1925Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The highly cross-linked 12% agarose gel Superose 12 10/300 GL Causes retardation of glycine peptides when mobile phases containing varying concentrations of acetonitrile in water are used An investigation has been made into the retention mechanism behind this retardation using the glycine dipeptide (GG) and tripeptide (GGG) as models The dependence of retention times of analytical-size peaks Under different experimental conditions was interpreted such that the adsorption most probably was caused by the formation of hydrogen bonds but that electrostatic interactions cannot be ruled Out Thereafter, a nonlinear adsorption study Was undertaken at different acetonitrile content in the eluent, using the elution by characteristic points (ECPs) method on strongly overloaded GG and GGG peaks With a new evaluation tool, the adsorption energy distribution (AED) Could be calculated prior to the model selection These calculations revealed that when the acetonitrile content in the eluent was varied from 0% to 20% the interactions turned from (i) being homogenous (GG) or mildly heterogeneous (GGG), (ii) via a more or less stronger degree of heterogeneity around one site to (iii) finally a typical bimodal energy interaction comprising of two sites (GG at 20% and GGG at 10% and 20%) The Langmuir, Toth and bi-Langmuir models described these interesting adsorption trends excellently Thus, the retardation observed for these glycine peptides is interpreted as being of mixed-mode character composed of electrostatic bonds and hydrogen bonds.

  • 9315.
    Zhang, Xinchen
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC.
    Interaction of PEG-ylated Lipid Nanoparticles with Silica Substrates2016Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [en]

    In this project, the interaction between polyethylene glycol modified (PEG-ylated) lipid nanoparticles and silica substrates was studied to find out how this interaction was affected by bulk concentration, temperature and the composition of particles. One kind of lipodisk and four kinds of PEG-ylated liposome were prepared from lipid films and characterized by quartz crystal microbalance with dissipation monitoring (QCM-D) instrument mounted with silica sensor. The detailed information of particle-silica interaction could be obtained from the raw data, frequency and dissipation values, and the adsorbed mass surface density calculated from the raw data. Lipodisks could be immobilized on the silica surface. Whether they would be rinsed away by PBS buffer was influenced by both the bulk concentration and temperature. The way of their binding could change and the changing process was affected by temperature. PEG-ylated liposomes could also be immobilized on the silica surface, and they could break and spread to form supported lipid bilayer in certain conditions, for example, the changing of temperature or the using of certain lipids. Supported lipid bilayers were created with high reproducibility in this project, which could be very useful to the future study of transmembrane proteins functions and lipodisk properties.

  • 9316.
    Zhang, Xudong
    et al.
    Qilu Univ Technol, Coll Mat Sci & Engn, Shandong Acad Sci, Jinan 250353, Shandong, Peoples R China.
    Bi, Zhiying
    Qilu Univ Technol, Coll Mat Sci & Engn, Shandong Acad Sci, Jinan 250353, Shandong, Peoples R China.
    Xu, Guogang
    Shandong Univ Sci & Technol, Coll Mat Sci & Engn, Qingdao 266590, Shandong, Peoples R China.
    Li, Changgang
    Qilu Univ Technol, Coll Mat Sci & Engn, Shandong Acad Sci, Jinan 250353, Shandong, Peoples R China.
    He, Wen
    Qilu Univ Technol, Coll Mat Sci & Engn, Shandong Acad Sci, Jinan 250353, Shandong, Peoples R China.
    Zhu, Jie-Fang
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Strukturkemi.
    Binary superlattice ceramic membrane-coated soft carbon/hard carbon microspheres for high energy mixed-ion batteries2019Ingår i: Journal of Power Sources, ISSN 0378-7753, E-ISSN 1873-2755, Vol. 438, artikel-id 226980Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The assembly of two different materials into a composite with binary superlattices can provide a most efficient and inexpensive path to the synthesis of electrode materials with high energy density. Here, Li9Fe3P8O29/Na2HPO4 binary superlattice ceramic membrane-coated mesoporous soft carbon/hard carbon microspheres (BSLCM-MSC/HCMs) are synthesized via a hydrothermal carbonization method using acyl nucleoside triphosphate disodium salt (Na(2)ATP) as a multifunctional template. Here Na(2)ATP is not only a phosphorus source, a sodium source and a nucleating agent for the rapid synthesis of BSLCM, but also is a carbon source which is easily transformed into MSC/HCMs after being heat-treated in a N-2 atmosphere. This special structure design brings a remarkable synergic effect for both Li+/Na+ mixed-ion storage and transfer kinetics. As a new nanocomposite cathode for mixed-ion batteries (MIBs), the BSLCM-MSC/HCMs display a self-enhancement of discharge capacity via electrolyte penetration in the core-shell mesoporous microspheres. Importantly, the discharge specific energy of a MIB assembled with the BSLCM-MSC/HCMs-2 cathode is up to 400 W h kg(-1) at a high current rate of 10C, which outperforms all lithium-ion batteries and sodium-ion batteries reported so far. The findings in this study may benefit to the development of high energy mixed-ion batteries.

  • 9317.
    Zhang, Xue-Ming
    et al.
    Beijing Forestry Univ, Coll Mat Sci & Technol, Beijing Key Lab Lignocellulos Chem, Beijing 100083, Peoples R China..
    Ma, Ming-Guo
    Beijing Forestry Univ, Coll Mat Sci & Technol, Beijing Key Lab Lignocellulos Chem, Beijing 100083, Peoples R China..
    Yang, Jun
    Univ British Columbia, Vancouver, BC, Canada..
    Xiang, Zhouyang
    Univ Wisconsin, Madison, WI USA..
    Zhu, Jie-Fang
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Strukturkemi.
    Alias, Yatimah
    Univ Malaya, Kuala Lumpur, Malaysia..
    Recent Advances in Cellulose-Based Materials: Synthesis, Characterization, and Their Applications2016Ingår i: International Journal of Polymer Science, ISSN 1687-9422, E-ISSN 1687-9430, artikel-id 8730573Artikel i tidskrift (Övrigt vetenskapligt)
  • 9318.
    Zhang, Yanxiao
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för naturvetenskaplig biokemi.
    Chromatographic studies of interactions between solutes, lipid bilayers and the glucose transporter Glut11997Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    It is advantageous to analyze solute interactions with biological membranes by chromatography on stationary phases that mimic the membrane structures. In the present work, liposomes, proteoliposomes, human red cells and red cell membrane vesicles were immobilized for analyses ofinteractions between solutes (peptides, drugs, inhibitors and glucose), lipid bilayers and theglucose transporter Glut1.

    For microscale analysis of solute-membrane interactions, liposomes were immobilized incontinuous beds for capillary chromatography and included in the running buffer as pseudostationary phase in capillary electrophoresis. These techniques were convenient and rapid. Inchromatographic drug partitioning studies, the logarithm of the specific capacity factors determined on liposomes in capillary continuous beds showed a linear correlation with the logarithmof apparent drug permeabilities through Caco-2 epithelial cell monolayers.

    Peptide and drug interactions with lipid bilayers were analyzed by chromatography on liposomes immobilized in gel beads. The retardations of peptides corresponding to segments ofGlut1 were related to the transfer free energy distribution within the peptides and to the hydrophobicities of the peptides. The effects of pH. temperature, ionic strength, flow rate and phospholipid composition on the retardation of drugs on liposomes or membrane vesicles were studied.

    For the first time, human red cells were immobilized in gel particles for chromatographicactivity analyses. D-Glucose showed a larger retardation than L-glucose upon transport retentionchromatography and Glut1 in the cells showed high affinities for D-glucose and forskolin, asdetermined by frontal affinity analyses.

    For exceptionally stable immobilization, biotin-avidin binding was introduced. Glut1 inproteoliposomes immobilized by this method showed solute affinities similar to those of sterically immobilized proteoliposomes.

  • 9319.
    Zhang, Yu
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström.
    Hyaluronan Based Biomaterials with Imaging Capacity for Tissue Engineering2016Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    This thesis presents the preparation of hyaluronan-based biomaterials with imaging capability and their application as scaffolds in tissue engineering. First, we have synthesized HA derivatives functionalized with different chemoselective groups. Then, functional ligands with capacities for hydrophobic drug loading, imaging, and metal ion coordination were chemically conjugated to HA by chemoselective reactions with these groups. An injectable in situ forming HA hydrogel was prepared by hydrazone cross-linking between hybrid iron-oxide nanogel and HA-aldehyde (paper-I). The degradation of this hydrogel could be monitored by MRI and UV-vis spectroscopy since it contained iron oxide as a contrast agent and pyrene as a fluorescent probe. Additionally, this hydrogel has a potential for a delivery of hydrophobic drugs due to its pyrene hydrophobic domains. The degradation study showed that degradability of the hydrogel was correlated with its structure. Based on the obtained results, disulfide cross-linked and fluorescently labeled hydrogels with different HA concentration were established as a model to study the relationship between the structure of the hydrogel and its degradability (paper-II). We demonstrated that disulfide cross-linked HA hydrogel could be tracked non-invasively by fluorescence imaging. It was proved that the in vivo degradation behavior of the hydrogel is predictable basing on its in vitro degradation study. In paper-III, we developed a disulfide cross-linked HA hydrogel for three-dimensional (3D) cell culture. In order to improve cell viability and adhesion to the matrix, HA derivatives were cross-linked in the presence of fibrinogen undergoing polymerization upon the action of thrombin. It led to the formation of an interpenetrating double network (IPN) of HA and fibrin. The results of 3D cell culture experiments revealed that the IPN hydrogel provides the cells with a more stable environment for proliferation. The results of the cellular studies were also supported by in vitro degradation of IPN monitored by fluorescence measurements of the degraded products. In paper-IV, the effect of biomineralization on hydrogel degradation was evaluated in a non-invasive manner in vitro. For this purpose, two types of fluorescently labeled hydrogels with the different ability for biomineralization were prepared. Fluorescence spectroscopy was applied to monitor degradation of the hydrogels in vitro under two different conditions in longitudinal studies. Under the supply of Ca2+ ions, the BP-modified hydrogel showed the tendency to bio-mineralization and reduction of the rate of degradation. Altogether, the performed studies showed the importance of imaging of hydrogel biomaterials in the design of optimized scaffolds for tissue engineering.

    Delarbeten
    1. Injectable In Situ Forming Hybrid Iron Oxide-Hyaluronic Acid Hydrogel for Magnetic Resonance Imaging and Drug Delivery
    Öppna denna publikation i ny flik eller fönster >>Injectable In Situ Forming Hybrid Iron Oxide-Hyaluronic Acid Hydrogel for Magnetic Resonance Imaging and Drug Delivery
    Visa övriga...
    2014 (Engelska)Ingår i: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 14, nr 9, s. 1249-1259Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    The development of multimodal in situ cross-linkable hyaluronic acid nanogels hybridized with iron oxide nanoparticles is reported. Utilizing a chemoselective hydrazone coupling reaction, the nanogels are converted to a macroscopic hybrid hydrogel without any additional reagent. Hydrophobic cargos remain encapsulated in the hydrophobic domains of the hybrid hydrogel without leakage. However, hydrogel degradation with hyaluronidase liberates iron oxide nanoparticles. This allows the utilization of imaging agents as tracers of the hydrogel degradation. UV-vis spectrometry and MRI studies reveal that the degradability of the hydrogels correlates with their structure. The hydrogels presented here are very promising theranostic tools for hyaluronidase-mediated delivery of hydrophobic drugs, as well as imaging of hydrogel degradation and tracking of degradation products in vivo.

    Nyckelord
    hybrid organic-inorganic nanogels, hyaluronan hydrogels, in situ cross-linking, iron oxide nanoparticles, magnetic resonance imaging
    Nationell ämneskategori
    Polymerkemi Biokemi och molekylärbiologi
    Identifikatorer
    urn:nbn:se:uu:diva-236090 (URN)10.1002/mabi.201400117 (DOI)000342921900005 ()
    Tillgänglig från: 2014-11-17 Skapad: 2014-11-12 Senast uppdaterad: 2017-12-05Bibliografiskt granskad
    2. Non-invasive in vitro and in vivo monitoring of degradation of fluorescently labeled hyaluronan hydrogels for tissue engineering applications
    Öppna denna publikation i ny flik eller fönster >>Non-invasive in vitro and in vivo monitoring of degradation of fluorescently labeled hyaluronan hydrogels for tissue engineering applications
    Visa övriga...
    2016 (Engelska)Ingår i: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 30, s. 188-198Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Tracking of degradation of hydrogels-based biomaterials in vivo is very important for rational design of tissue engineering scaffolds that act as delivery carriers for bioactive factors. During the process of tissue development, an ideal scaffold should remodel at a rate matching with scaffold degradation. To reduce amount of animals sacrificed, non-invasive in vivo imaging of biomaterials is required which relies on using of biocompatible and in situ gel forming compounds carrying suitable imaging agents. In this study we developed a method of in situ fabrication of fluorescently labeled and injectable hyaluronan (HA) hydrogel based on one pot sequential use of Michael addition and thiol-disulfide exchange reactions for the macromolecules labeling and cross-linking respectively. Hydrogels with different content of HA were prepared and their enzymatic degradation was followed in vitro and in vivo using fluorescence multispectral imaging. First, we confirmed that the absorbance of the matrix-linked near-IR fluorescent IRDye (R) 800CW agent released due to the matrix enzymatic degradation in vitro matched the amount of the degraded hydrogel measured by classical gravimetric method. Secondly, the rate of degradation was inversely proportional to the hydrogel concentration and this structure-degradation relationship was similar for both in vitro and in vivo studies. It implies that the degradation of this disulfide cross-linked hyaluronan hydrogel in vivo can be predicted basing on the results of its in vitro degradation studies. The compliance of in vitro and in vivo methods is also promising for the future development of predictive in vitro tissue engineering models. Statement of significance The need for engineered hydrogel scaffolds that deliver bioactive factors to endogenous progenitor cells in vivo via gradual matrix resorption and thus facilitate tissue regeneration is increasing with the aging population. Importantly, scaffold should degrade at a modest rate that will not be too fast to support tissue growth nor too slow to provide space for tissue development. The present work is devoted to longitudinal tracking of a hydrogel material in vivo from the time of its implantation to the time of complete resorption without sacrificing animals. The method demonstrates correlation of resorption rates in vivo and in vitro for hydrogels with varied structural parameters. It opens the possibility to develop predictive in vitro models for tissue engineered scaffolds and reduce animal studies.

    Nyckelord
    Hyaluronan, Hydrogel degradation, Non-invasive imaging, Spinal cord, Tissue engineering
    Nationell ämneskategori
    Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
    Identifikatorer
    urn:nbn:se:uu:diva-277793 (URN)10.1016/j.actbio.2015.11.053 (DOI)000368563600017 ()26621694 (PubMedID)
    Forskningsfinansiär
    EU, FP7, Sjunde ramprogrammet
    Anmärkning

    De två första författarna delar förstaförfattarskapet.

    Tillgänglig från: 2016-02-23 Skapad: 2016-02-23 Senast uppdaterad: 2017-11-30Bibliografiskt granskad
    3. Hyaluronic acid-fibrin interpenetrating double network hydrogel prepared in situ by orthogonal disulfide cross-linking reaction for biomedical applications
    Öppna denna publikation i ny flik eller fönster >>Hyaluronic acid-fibrin interpenetrating double network hydrogel prepared in situ by orthogonal disulfide cross-linking reaction for biomedical applications
    Visa övriga...
    2016 (Engelska)Ingår i: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 38, s. 23-32Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking. The synthetic pathway included the preparation of mutually reactive HA derivatives bearing thiol and 2-dithiopyridyl groups. Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks. The formation of two networks was confirmed by conducting reference experiments with the compositions lacking one of the four components. The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts. The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels. Together with the persistence of HA network, it permitted longer cell culturing time in the IPN. Moreover, the proliferation and spreading of MG63 cells that express the hyaluronan receptor CD44 in IPN hydrogel was increased, as compared with its single network analogues. These results are promising for tunable ECM-based materials for TE and regenerative medicine. Statement of Significance The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other. This is accomplished by combination of enzymatic fibrin cross-linking with orthogonal disulphide cross-linking of HA. High hydrophilicity of HA prevents compaction of the fibrin network, while fibrin provides an adhesive environment for in situ encapsulated cells. The interpenetrating network hydrogel shows an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network. As a result, the cells have sufficient time for the remodelling of the scaffold. This new approach can be applied for modular construction of in vitro tissue models and tissue engineering scaffolds in vivo.

    Nyckelord
    Interpenetrating hydrogel, Fibrin, Hyaluronic acid, Biodegradation, 3D cell culture
    Nationell ämneskategori
    Polymerkemi Biomaterial
    Identifikatorer
    urn:nbn:se:uu:diva-300044 (URN)10.1016/j.actbio.2016.04.041 (DOI)000378963800003 ()27134013 (PubMedID)
    Forskningsfinansiär
    EU, Europeiska forskningsrådet
    Tillgänglig från: 2016-08-02 Skapad: 2016-08-02 Senast uppdaterad: 2017-11-28Bibliografiskt granskad
    4. Enzymatic degradation of hyaluronan hydrogels with different capacity for in situ bio-mineralization
    Öppna denna publikation i ny flik eller fönster >>Enzymatic degradation of hyaluronan hydrogels with different capacity for in situ bio-mineralization
    (Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
    Nationell ämneskategori
    Naturvetenskap Polymerkemi
    Identifikatorer
    urn:nbn:se:uu:diva-300791 (URN)
    Tillgänglig från: 2016-08-14 Skapad: 2016-08-13 Senast uppdaterad: 2016-09-02
  • 9320.
    ZHANG, YU
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Heher, Philipp
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi. Austrian Cluster for Tissue Regeneration, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology.
    Hilborn, Jöns
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Redl, Heinz
    g Boltzmann Institute for Experimental and Clinical Traumatology.
    Ossipov, Dmitri
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Hyaluronic acid-fibrin interpenetrating double network hydrogelprepared in situ by orthogonal disulfide cross-linking reaction forbiomedical applications2016Ingår i: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568Artikel i tidskrift (Refereegranskat)
  • 9321.
    Zhang, Yu
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Heher, Philipp
    Ludwig Boltzmann Inst Expt & Clin Traumatol, Austrian Cluster Tissue Regenerat, Donaueschingenstr 13, A-1200 Vienna, Austria.;Trauma Care Consult GmbH, Gonzagagasse 11-25, A-1010 Vienna, Austria..
    Hilborn, Jöns
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Redl, Heinz
    Ludwig Boltzmann Inst Expt & Clin Traumatol, Austrian Cluster Tissue Regenerat, Donaueschingenstr 13, A-1200 Vienna, Austria.;Trauma Care Consult GmbH, Gonzagagasse 11-25, A-1010 Vienna, Austria..
    Ossipov, Dmitri A.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Hyaluronic acid-fibrin interpenetrating double network hydrogel prepared in situ by orthogonal disulfide cross-linking reaction for biomedical applications2016Ingår i: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 38, s. 23-32Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking. The synthetic pathway included the preparation of mutually reactive HA derivatives bearing thiol and 2-dithiopyridyl groups. Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks. The formation of two networks was confirmed by conducting reference experiments with the compositions lacking one of the four components. The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts. The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels. Together with the persistence of HA network, it permitted longer cell culturing time in the IPN. Moreover, the proliferation and spreading of MG63 cells that express the hyaluronan receptor CD44 in IPN hydrogel was increased, as compared with its single network analogues. These results are promising for tunable ECM-based materials for TE and regenerative medicine. Statement of Significance The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other. This is accomplished by combination of enzymatic fibrin cross-linking with orthogonal disulphide cross-linking of HA. High hydrophilicity of HA prevents compaction of the fibrin network, while fibrin provides an adhesive environment for in situ encapsulated cells. The interpenetrating network hydrogel shows an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network. As a result, the cells have sufficient time for the remodelling of the scaffold. This new approach can be applied for modular construction of in vitro tissue models and tissue engineering scaffolds in vivo.

  • 9322.
    Zhang, Yu
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Rossi, Filippo
    Politecn Milan, Dept Chem Mat & Chem Engn Giulio Natta, Via Mancinelli, I-20131 Milan, Italy..
    Papa, Simonetta
    IRCCS Ist Ric Farmacol Mario Negri, Dept Neurosci, Via La Masa 19, I-20156 Milan, Italy..
    Violatto, Martina Bruna
    IRCCS Ist Ric Farmacol Mario Negri, Dept Biochem & Mol Pharmacol, Via La Masa 19, I-20156 Milan, Italy..
    Bigini, Paolo
    IRCCS Ist Ric Farmacol Mario Negri, Dept Biochem & Mol Pharmacol, Via La Masa 19, I-20156 Milan, Italy..
    Sorbona, Marco
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Redaelli, Francesca
    Veglianese, Pietro
    IRCCS Ist Ric Farmacol Mario Negri, Dept Neurosci, Via La Masa 19, I-20156 Milan, Italy..
    Hilborn, Jöns
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Ossipov, Dmitri A.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Non-invasive in vitro and in vivo monitoring of degradation of fluorescently labeled hyaluronan hydrogels for tissue engineering applications2016Ingår i: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 30, s. 188-198Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Tracking of degradation of hydrogels-based biomaterials in vivo is very important for rational design of tissue engineering scaffolds that act as delivery carriers for bioactive factors. During the process of tissue development, an ideal scaffold should remodel at a rate matching with scaffold degradation. To reduce amount of animals sacrificed, non-invasive in vivo imaging of biomaterials is required which relies on using of biocompatible and in situ gel forming compounds carrying suitable imaging agents. In this study we developed a method of in situ fabrication of fluorescently labeled and injectable hyaluronan (HA) hydrogel based on one pot sequential use of Michael addition and thiol-disulfide exchange reactions for the macromolecules labeling and cross-linking respectively. Hydrogels with different content of HA were prepared and their enzymatic degradation was followed in vitro and in vivo using fluorescence multispectral imaging. First, we confirmed that the absorbance of the matrix-linked near-IR fluorescent IRDye (R) 800CW agent released due to the matrix enzymatic degradation in vitro matched the amount of the degraded hydrogel measured by classical gravimetric method. Secondly, the rate of degradation was inversely proportional to the hydrogel concentration and this structure-degradation relationship was similar for both in vitro and in vivo studies. It implies that the degradation of this disulfide cross-linked hyaluronan hydrogel in vivo can be predicted basing on the results of its in vitro degradation studies. The compliance of in vitro and in vivo methods is also promising for the future development of predictive in vitro tissue engineering models. Statement of significance The need for engineered hydrogel scaffolds that deliver bioactive factors to endogenous progenitor cells in vivo via gradual matrix resorption and thus facilitate tissue regeneration is increasing with the aging population. Importantly, scaffold should degrade at a modest rate that will not be too fast to support tissue growth nor too slow to provide space for tissue development. The present work is devoted to longitudinal tracking of a hydrogel material in vivo from the time of its implantation to the time of complete resorption without sacrificing animals. The method demonstrates correlation of resorption rates in vivo and in vitro for hydrogels with varied structural parameters. It opens the possibility to develop predictive in vitro models for tissue engineered scaffolds and reduce animal studies.

  • 9323.
    ZHANG, YU
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström.
    Shi, Liyang
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Hilborn, Jöns
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Ossipov, Dmitri
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Enzymatic degradation of hyaluronan hydrogels with different capacity for in situ bio-mineralizationManuskript (preprint) (Övrigt vetenskapligt)
  • 9324.
    Zhang, Yu
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi. Uppsala universitet, Science for Life Laboratory, SciLifeLab.
    Sun, Yi
    Yang, Xia
    Hilborn, Jöns
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi. Uppsala universitet, Science for Life Laboratory, SciLifeLab.
    Heerschap, Arend
    Ossipov, Dmitri A.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi. Uppsala universitet, Science for Life Laboratory, SciLifeLab.
    Injectable In Situ Forming Hybrid Iron Oxide-Hyaluronic Acid Hydrogel for Magnetic Resonance Imaging and Drug Delivery2014Ingår i: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 14, nr 9, s. 1249-1259Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The development of multimodal in situ cross-linkable hyaluronic acid nanogels hybridized with iron oxide nanoparticles is reported. Utilizing a chemoselective hydrazone coupling reaction, the nanogels are converted to a macroscopic hybrid hydrogel without any additional reagent. Hydrophobic cargos remain encapsulated in the hydrophobic domains of the hybrid hydrogel without leakage. However, hydrogel degradation with hyaluronidase liberates iron oxide nanoparticles. This allows the utilization of imaging agents as tracers of the hydrogel degradation. UV-vis spectrometry and MRI studies reveal that the degradability of the hydrogels correlates with their structure. The hydrogels presented here are very promising theranostic tools for hyaluronidase-mediated delivery of hydrophobic drugs, as well as imaging of hydrogel degradation and tracking of degradation products in vivo.

  • 9325.
    Zhang, Yuan
    et al.
    Binzhou Med Univ, Med & Pharm Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China.
    Wang, Dan
    Binzhou Med Univ, Med & Pharm Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China;Binzhou Med Univ, Affiliated Hosp, Dept Radiol, 661 Second Huanghe Rd, Binzhou 256603, Shandong, Peoples R China.
    Li, Min
    Binzhou Med Univ, Med & Pharm Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China.
    Wei, Xiaodan
    Binzhou Med Univ, Med & Pharm Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China.
    Liu, Shuang
    Binzhou Med Univ, Coll Enol, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China.
    Zhao, Miaoqing
    Shandong Univ, Prov Hosp, Dept Pathol, 324 Jingwu Weiqi Rd, Jinan 250021, Shandong, Peoples R China.
    Liu, Chu
    Yantai Yuhuangding Hosp, Dept Urol, 20 Yuhuangding East Rd, Yantai 264000, Shandong, Peoples R China.
    Wang, Xizhen
    Weifang Med Univ, Affiliated Hosp, Imaging Ctr, 465 Yuhe Rd, Weifang 256603, Shandong, Peoples R China.
    Jiang, Xingyue
    Binzhou Med Univ, Affiliated Hosp, Dept Radiol, 661 Second Huanghe Rd, Binzhou 256603, Shandong, Peoples R China.
    Li, Xuri
    Binzhou Med Univ, Med & Pharm Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China.
    Zhang, Shuping
    Binzhou Med Univ, Med & Pharm Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China.
    Bergquist, Jonas
    Binzhou Med Univ, Med & Pharm Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China.
    Wang, Bin
    Binzhou Med Univ, Med & Pharm Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China.
    Yang, Chunhua
    Binzhou Med Univ, Med & Pharm Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China.
    Mi, Jia
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi. Binzhou Med Univ, Med & Pharm Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China.
    Tian, Geng
    Binzhou Med Univ, Med & Pharm Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China.
    Quantitative Proteomics of TRAMP Mice Combined with Bioinformatics Analysis Reveals That PDGF-B Regulatory Network Plays a Key Role in Prostate Cancer Progression2018Ingår i: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 17, nr 7, s. 2401-2411Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Transgenic adenocarcinoma of the mouse prostate (TRAMP) mice is a widely used transgenic animal model of prostate cancer (PCa). We performed a label-free quantitative proteomics analysis combined with a bioinformatics analysis on the entire prostate protein extraction from TRAMP mice and compared it with WT littermates. From 2379 total identified proteins, we presented a modest mice prostate reference proteome containing 919 proteins. 61 proteins presented a significant expression difference between two groups. The integrative bioinformatics analysis predicted the overexpression of platelet-derived growth factor B (PDGF-B) in tumor tissues and supports the hypothesis of the PDGF-B signaling network as a key upstream regulator in PCa progression. Furthermore, we demonstrated that Crenolanib, a novel PDGF receptor inhibitor, inhibited PCa cell proliferation in a dose-dependent manner. Finally, we revealed the importance of PDGF-B regulatory network in PCa progression, which will assist us in understanding the role and mechanisms of PDGF-B in promoting cancer growth and provide valuable knowledge for future research on anti-PDGF therapy.

  • 9326.
    Zhansheng, Lu
    et al.
    College of Physics and Information Engineering, Henan Normal University, Xinxiang, Henan 453007, China.
    Kullgren, Jolla
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Strukturkemi.
    Yang, Zongxian
    College of Physics and Information Engineering, Henan Normal University, Xinxiang, Henan 453007, China.
    Hermansson, Kersti
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Strukturkemi.
    Sulfidation of ceria surfaces from sulfur and sulfur diffusion2012Ingår i: The Journal of Physical Chemistry C, ISSN 1932-7447, E-ISSN 1932-7455, Vol. 116, nr 15, s. 8417-8425Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Even very low levels of sulfur contaminants can degrade the catalytic performance of cerium oxide. Here, the interaction of atomic sulfur with the ceria (111) and (110) surfaces has been studied using first-principles methods. Two sulfoxy species are identified: oxido-sulfate(2-) species (SO2-) on both the CeO2 (111) and (110) surfaces and hyposulfite (SO22-) on the (110) surface. Sulfide (S2-) is formed when a surface or a subsurface oxygen atoms is replaced by sulfur. These sulfide species are most stable at the surface. Furthermore, sulfite (SO32-) structures are found when sulfur is made to replaces one Ce in the ceria (111) and (110) surfaces. The calculated sulfur diffusion barriers are larger than 1.4 eV for both surfaces and thus sulfur is essentially immobile, providing a possible explanation for the sulfidation phenomena of the ceria-based catalysis. Thus we find three different species from interaction of S with Ceria which are all, due to their strong binding, capable of poisoning the surface, reduced or unreduced. Our results suggest that under reducing conditions, sulfur is likely to be found in the (111) surface (replacing oxygen) but on the (110) surface (as SO22-).

  • 9327.
    Zhao, Hongxing
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Molekylära verktyg.
    Chen, Maoshan
    Monash Univ, Cent Clin Sch, Australian Ctr Blood Dis, Clayton, Vic, Australia.
    Valdes, Alberto
    Univ Alcala De Henares, Dept Analyt Chem Phys Chem & Chem Engn, Madrid, Spain.
    Lind, Sara
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
    Pettersson, Ulf
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Medicinsk genetik och genomik.
    Transcriptomic and proteomic analyses reveal new insights into the regulation of immune pathways during adenovirus type 2 infection2019Ingår i: BMC Microbiology, ISSN 1471-2180, E-ISSN 1471-2180, Vol. 19, artikel-id 15Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: Human adenovirus (Ad) infection leads to the changes of host cell gene expression and biosynthetic processes. Transcriptomics in adenovirus type 2 (Ad2)-infected lung fibroblasts (IMR-90) cells has previously been studied using RNA sequencing. However, this study included only two time points (12 and 24 hpi) using constrained 76bp long sequencing reads. Therefore, a more detailed study of transcription at different phases of infection using an up-graded sequencing technique is recalled. Furthermore, the correlation between transcription and protein expression needs to be addressed.

    Results: In total, 3556 unique cellular genes were identified as differentially expressed at the transcriptional level with more than 2-fold changes in Ad2-infected cells as compared to non-infected cells by using paired-end sequencing. Based on the kinetics of the gene expression changes at different times after infection, these RNAs fell into 20 clusters. Among them, cellular genes involved in immune response were highly up-regulated in the early phase before becoming down-regulated in the late phase. Comparison of differentially expressed genes at transcriptional and posttranscriptional levels revealed low correlation. Particularly genes involved in cellular immune pathways showed a negative correlation. Here, we highlight the genes which expose inconsistent expression profiles with an emphasis on key factors in cellular immune pathways including NFB, JAK/STAT, caspases and MAVS. Different from their transcriptional profiles with up- and down-regulation in the early and late phase, respectively, these proteins were up-regulated in the early phase and were sustained in the late phase. A surprising finding was that the target genes of the sustained activators failed to show response.

    Conclusion: There were features common to genes which play important roles in cellular immune pathways. Their expression was stimulated at both RNA and protein levels during the early phase. In the late phase however, their transcription was suppressed while protein levels remained stable. These results indicate that Ad2 and the host cell use different strategies to regulate cellular immune pathways. A control mechanism at the post-translational level must thus exist which is under the control of Ad2.

  • 9328.
    Zhao, Hongxing
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Medicinsk genetik och genomik.
    Chen, Moashan
    Bergström Lind, Sara
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
    Pettersson, Ulf
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Medicinsk genetik och genomik.
    Distinct temporal changes in host cell lncRNA expression during the course of an adenovirus infection2016Ingår i: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 492, s. 242-250Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The deregulation of cellular long non-coding RNA (lncRNA) expression during a human adenovirus infection was studied by deep sequencing. Expression of lncRNAs increased substantially following the progression of the infection. Among 645 significantly expressed lncRNAs, the expression of 398 was changed more than 2-fold. More than 80% of them were up-regulated and 80% of them were detected during the late phase. Eased on the genomic locations of the deregulated lncRNAs in relation to known mRNAs and miRNAs, they were predicted to be involved in growth, structure, apoptosis and wound healing in the early phase, cell proliferation in the intermediate phase and protein synthesis, modification and transport in the late phase. The most significant functions of cellular RNA-binding proteins, previously shown to interact with the deregulated lncRNAs identified here, are involved in RNA splicing, nuclear export and translation events. We hypothesize that adenoviruses exploit the lncRNA network to optimize their reproduction.

  • 9329.
    Zhao, Hongxing
    et al.
    Uppsala universitet, Science for Life Laboratory, SciLifeLab. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi.
    Konzer, Anne
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
    Mi, Jia
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
    Chen, Moashan
    La Trobe University.
    Pettersson, Ulf
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi.
    Lind, Sara Bergström
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Analytisk kemi.
    Posttranscriptional regulation in adenovirus infected cells2017Ingår i: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 16, nr 2, s. 872-888Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A deeper understanding of how viruses reprogramtheir hosts for production of progeny is needed to combatinfections. Most knowledge on the regulation of cellular geneexpression during adenovirus infection is derived from mRNAstudies. Here, we investigated the changes in protein expressionduring the late phase of adenovirus type 2 (Ad2) infection of theIMR-90 cell line by stable isotope labeling in cell culture withsubsequent liquid chromatography−high resolution tandemmass spectrometric analysis. Two biological replicates of samplescollected at 24 and 36 h post-infection (hpi) were investigated using swapped labeling. In total, 2648 and 2394 proteins werequantified at 24 and 36 hpi, respectively. Among them, 659 and 645 were deregulated >1.6-fold at the two time points. Theprotein expression was compared with RNA expression using cDNA sequencing data. The correlation was surprisingly low(r = 0.3), and several examples of posttranscriptional regulation were observed; e.g., proteins related to carbohydrate metabolismwere up-regulated at the protein level but unchanged at the RNA level, whereas histone proteins were down-regulated at theprotein level but up-regulated at the RNA level. The deregulation of cellular gene expression by adenovirus is mediated atmultiple levels and more complex than hitherto believed.

  • 9330. Zhao, J.
    et al.
    Zeiai, S.
    Ekblad, A.
    Nordenskjold, A.
    Hilborn, Jöns
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi. Uppsala universitet, Science for Life Laboratory, SciLifeLab.
    Gotherstrom, C.
    Fossum, M.
    Transdifferentiation of autologous bone marrow cells on a collagen-poly(epsilon-caprolactone) scaffold for tissue engineering in complete lack of native urothelium2014Ingår i: Journal of the Royal Society Interface, ISSN 1742-5689, E-ISSN 1742-5662, Vol. 11, nr 96Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Urological reconstructive surgery is sometimes hampered by a lack of tissue. In some cases, autologous urothelial cells (UCs) are not available for cell expansion and ordinary tissue engineering. In these cases, we wanted to explore whether autologous mesenchymal stem cells (MSCs) from bone marrow could be used to create urological transplants. MSCs from human bone marrow were cultured in vitro with medium conditioned by normal human UCs or by indirect co-culturing in culture well inserts. Changes in gene expression, protein expression and cell morphology were studied after two weeks using western blot, RT-PCR and immune staining. Cells cultured in standard epithelial growth medium served as controls. Bone marrow MSCs changed their phenotype with respect to growth characteristics and cell morphology, as well as gene and protein expression, to a UC lineage in both culture methods, but not in controls. Urothelial differentiation was also accomplished in human bone marrow MSCs seeded on a three-dimensional poly(epsilon-caprolactone) (PCL)-collagen construct. Human MSCs could easily be harvested by bone marrow aspiration and expanded and differentiated into urothelium. Differentiation could take place on a three-dimensional hybrid PCL-reinforced collagen-based scaffold for creation of a tissue-engineered autologous transplant for urological reconstructive surgery.

  • 9331. Zhao, Jianghua
    et al.
    Yang, Xichuan
    Hao, Yan
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Fysikalisk kemi.
    Cheng, Ming
    Tian, Jie
    Sun, Licheng
    Effect of Different Numbers of -CH2- Units on the Performance of Isoquinolinium Dyes2014Ingår i: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 6, nr 6, s. 3907-3914Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Three new dyes have been synthesized to investigate the influence of the distance between the electron acceptor and TiO2 surface on the performance of dye-sensitized solar cells (DSSCs). In these dyes, the isoquinolinium acceptor, with a -(CH2)(n) COOH anchoring group, and a functionalized triphenylamine donor are separated by an oligothiophene bridge. The physical and electrochemical properties of the dyes were investigated systematically. The results prove that different numbers of -CH2- units between the isoquinolinium acceptor and the carboxyl anchoring group have a less pronounced effect on the physical and electrochemical properties of these dyes. However, when applied in DSSCs, a sharp decrease in the short-circuit current (J(SC)) was observed with increasing numbers of -CH2- units. For example, the device containing the organic dye bearing three -CH2- units produced the lowest J(SC) of 7.94 mA.cm(-2). In contrast, the device containing the dye bearing only one -CH2- unit exhibited the highest J(SC) of 13.88 mA.cm(-2). The higher photocurrent obtained with the device incorporating the dye with one -CH2- unit resulted in a higher power conversion efficiency of 6.8%.

  • 9332.
    Zhao, Jie
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets elektronik.
    Pan, Ruijun
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Oorganisk kemi.
    Sun, Rui
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Nanoteknologi och funktionella material.
    Wen, Chenyu
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets elektronik.
    Zhang, Shi-Li
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets elektronik.
    Wu, Biao
    Nyholm, Leif
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Oorganisk kemi.
    Zhang, Zhi-Bin
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets elektronik.
    High-Conductivity Reduced-Graphene-Oxide/Copper Aerogel for Energy Storage2019Ingår i: Nano Energy, ISSN 2211-2855, E-ISSN 2211-3282, Vol. 60, s. 760-767Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    This work reports a room-temperature, solution-phase and one-pot method for macro-assembly of a three-dimensional (3D) reduced-graphene-oxide/copper hybrid hydrogel. The hydrogel is subsequently transformed into a highly conductive aerogel via freeze-drying. The aerogel, featuring reduced graphene oxide (rGO) networks decorated with Cu and CuxO nanoparticles (Cu/CuxO@rGO), exhibits a specific surface area of 48 m(2)/g and an apparent electrical conductivity of similar to 33 and similar to 430 S/m prior to and after mechanical compression, respectively. The compressed Cu/CuxO@rGO aerogel delivers a specific capacity of similar to 453 mAh g(-1) at a current density of 1 A/g and similar to 184 mAh g(-1) at 50 A/g in a 3 M KOH aqueous electrolyte evidenced by electrochemical measurements. Galvanostatic cycling tests at 5 A/g demonstrates that the Cu/CuxO@rGO aerogel retains 38% (similar to 129 mAh g(-1)) of the initial capacity (similar to 339 mAh g(-1)) after 500 cycles. The straightforward manufacturing process and the promising electrochemical performances make the Cu/CuxO@rGO aerogel an attractive electrode candidate in energy storage applications.

  • 9333.
    Zhao, Shuainan
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Oorganisk kemi. Department of Chemistry - Ångström Laboratory.
    Theoretical Studies of Diamond for Electronic Applications2016Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Diamond has since many years been applied in electronic fields due to its extraordinary properties. Substitutional dopants and surface functionalization have also been introduced in order to improve the electrochemical properties. However, the basic mechanism at an atomic level, regarding the effects of dopants and terminations, is still under debate. In addition, theoretical modelling has during the last decades been widely used for the interpretation of experimental results, prediction of material properties, and for the guidance of future materials. Therefore, the purpose of this research project has been to theoretically investigate the influence of dopants and adsorbates on electronic and geometrical structures by using density functional theory (DFT) under periodic boundary conditions.

    Both the global and local effects of dopants (boron and phosphorous) and terminations have been studied. The models have included H-, OH-, F-, Oontop-, Obridge- and NH2-terminations on the diamond surfaces. For all terminating species studied, both boron and phosphorous have been found to show a local impact, instead of a global one, on diamond structural geometry and electronic properties. Therefore, the terminating species only affect the DOS of the surface carbon layers. In addition, Oontop-terminated (111) diamond surfaces present reactive surface properties and display metallic conductivity. Moreover, the conductivity of the diamond surface can be dramatically increased by the introduction of a phosphorous dopant in the lattice. The work function of a diamond surface has also been found to be influenced to a large extent by the various adsorbates and the dopant levels.

    Diamond can also be used as a promising substrate for an epitaxial graphene adlayer. The effects of dopants and terminations on the graphene and diamond (111) interfacial systems have been investigated theoretically in great detail. The interfacial interaction is of the Van der Waal type with an interfacial distance around 3 Å. The interactions between graphene and a terminated diamond substrate were found to be relatively weaker than those for a non-terminated diamond substrate (even with dopants). For all interface systems between graphene and diamond, a diamond-supported graphene adlayer without induced defects can still keep its intrinsic high carrier mobility. A minor charge transfer was observed to take place from the graphene adlayer to a non-terminated diamond substrate (with or without dopants) and to Oontop-, OH- or Obridge-terminated diamond substrates. However, for the situation with an H-terminated diamond surface, the electron transfer took place from the diamond surface to graphene. On the contrary, an interfacial system with a non-terminated diamond surface offers a more pronounced charge transfer than that of the terminated diamond substrates. A small finite band gap at the Dirac point was also observed for the Oontop-terminated diamond-supporting graphene adlayer.

     

     

     

    Delarbeten
    1. A Theoretical Study of the Energetic Stability and Geometry of Terminated and B-Doped Diamond (111) Surfaces
    Öppna denna publikation i ny flik eller fönster >>A Theoretical Study of the Energetic Stability and Geometry of Terminated and B-Doped Diamond (111) Surfaces
    2014 (Engelska)Ingår i: The Journal of Physical Chemistry C, ISSN 1932-7447, E-ISSN 1932-7455, Vol. 118, nr 4, s. 1944-1957Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    The effect of B doping on the surface (111) reactivity has, in the present study, been investigated for various surface terminations, H, OH, Oon-top and F. This type of surface modification has experimentally been proven to be extremely important for, for example, applications based on surface electrochemistry. Density functional theory (DFT) has here been used to study both the local and more global effects of substitutionally positioned B atoms in the upper part of the diamond (111) surface. For this purpose, adsorption energies for the various terminating species have been calculated, and the observed results have been carefully analyzed in order to gain a deeper knowledge about the atomic-level cause of the observed effects. As a result, the B dopant shows a clear, but local, effect for all terminating species investigated. In addition, it is only the radical O-terminating species that show a special and high reactivity on the diamond surface. The other terminating species show a much lower reactivity, which in addition are very similar.

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    Naturvetenskap
    Identifikatorer
    urn:nbn:se:uu:diva-220298 (URN)10.1021/jp409278x (DOI)000330610200023 ()
    Tillgänglig från: 2014-03-17 Skapad: 2014-03-12 Senast uppdaterad: 2017-12-05
    2. A Theoretical Study of the Energetic Stability and Electronic Structure of Terminated and P-doped Diamond (100)-2x1 Surfaces
    Öppna denna publikation i ny flik eller fönster >>A Theoretical Study of the Energetic Stability and Electronic Structure of Terminated and P-doped Diamond (100)-2x1 Surfaces
    (Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
    Nationell ämneskategori
    Oorganisk kemi
    Identifikatorer
    urn:nbn:se:uu:diva-283407 (URN)
    Tillgänglig från: 2016-04-12 Skapad: 2016-04-12 Senast uppdaterad: 2016-06-01Bibliografiskt granskad
    3. First principle study of the attachment of graphene onto non-doped and doped diamond (111)
    Öppna denna publikation i ny flik eller fönster >>First principle study of the attachment of graphene onto non-doped and doped diamond (111)
    2016 (Engelska)Ingår i: Diamond and related materials, ISSN 0925-9635, E-ISSN 1879-0062, Vol. 66, s. 52-60Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Density function theory (DFT) calculations have in the present study been used to study the adhesion of a graphene monolayer onto a non-, B-, or N-doped diamond (111) surface. Semiempirical dispersion corrections were used to take the Van-der-Waals corrections into consideration. In case of non-doped diamond as a substrate, DFT calculations (based on the local density approximation (LDA)) have shown a strong binding between graphene and the diamond (111) surface at a shorter distance (2.47 Å). The binding energy was − 14.5 kJ/mol per Cgraphene atom. In comparison, the generalized gradient spin density approximation (GG(S)A) was found to predict a weaker