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  • 1. Agianian, Bogos
    et al.
    Lesch, Christine
    Loseva, Olga
    Dushay, Mitchell S.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Preliminary characterization of hemolymph coagulation in Anopheles gambiae larvae2007In: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 31, no 9, p. 879-888Article in journal (Refereed)
    Abstract [en]

    Hemolymph coagulation is a first response to injury, impeding infection, and ending bleeding. Little is known about its molecular basis in insects, but clotting factors have been identified in the fruit fly Drosophila melanogaster. Here, we have begun to study coagulation in the aquatic larvae of the malaria vector mosquito Anopheles gambiae using methods developed for Drosophila. A delicate clot was seen by light microscopy, and pullout and proteomic analysis identified phenoloxidase and apolipophorin-I as major candidate clotting factors. Electron microscopic analysis confirmed clot formation and revealed it contains fine molecular sheets, most likely a result of lipophorin assembly. Phenoloxidase appears to be more critical in clot formation in Anopheles than in Drosophila. The Anopheles larval clot thus differs in formation, structure, and composition from the clot in Drosophila, confirming the need to study coagulation in different insect species to learn more about its evolution and adaptation to different lifestyles.

  • 2.
    Andersson, M Gunnar
    et al.
    Jämförande fysiologi.
    Cerenius, Lage
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Analysis of chitinase expression in the crayfish plague fungus Aphanomyces astaci.2002In: Dis Aquat Organ, ISSN 0177-5103, Vol. 51, no 2, p. 139-47Article in journal (Other scientific)
  • 3.
    Andersson, M Gunnar
    et al.
    Jämförande fysiologi.
    Cerenius, Lage
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Pumilio homologue from saprolegnia parasitica specifically expressed in undifferentiated spore cysts.2002In: Eukaryot Cell, ISSN 1535-9778, Vol. 1, no 1, p. 105-11Article in journal (Other scientific)
  • 4.
    Andrén, Tove
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Hematopoietic stem cell differentiation in crustaceans2004Licentiate thesis, monograph (Other scientific)
  • 5.
    Aspan, A
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Huang, TS
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Cerenius, Lage
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    CDNA CLONING OF PROPHENOLOXIDASE FROM THE FRESH-WATER CRAYFISH PACIFASTACUS-LENIUSCULUS AND ITS ACTIVATION1995In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 92, no 4, p. 939-943Article in journal (Refereed)
    Abstract [en]

    Prophenoloxidase (proPO), an enzyme that is the terminal component of the so-called proPO activating system, a defense and recognition system in crustaceans and insects, has been purified and cloned from a crayfish blood cell cDNA library. The deduced ami

  • 6. Aspan, Anna
    et al.
    Söderhäll, Kenneth
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    The prophenoloxidase activating system in invertebrates; assays of the prophenoloxidase activating enzyme and phenoloxidase.1995In: Techniques in Fish Immunology 4, 161-171, 1995Chapter in book (Refereed)
  • 7. Avarre, JC
    et al.
    Michelis, R
    Hall, M
    Söderhäll, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    Khayat, M
    Tietz, A
    Lubzens, E
    Lipid composition during sexual development of the noble crayfish Asyacus astacus and effect of a fungal infection2002In: Invertebrate Reproduction and Development, Vol. 41, no 1-3, p. 251-259Article in journal (Refereed)
  • 8. Bachere, E
    et al.
    Fuchs, R
    Söderhäll, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Shrimp immunity and disease control2000In: AQUACULTUREArticle in journal (Refereed)
  • 9.
    Backström, Tobias
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Roles of Arginine-Vasotocin and Corticotropin-Releasing Hormone in Stress Responses and Agonistic Behaviour of Rainbow Trout2008Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    The neuropeptides arginine-vasotocin (AVT) and corticotropin-releasing hormone (CRH) are involved in the hypothalamic-pituitary-interrenal (HPI) axis. During stress, the HPI axis is activated and cortisol is released into the blood. In addition to their role in the HPI axis, AVT and CRH also have behavioural effects. The roles of AVT and CRH in stress responses and agonistic behaviour were studied in this thesis, using two different models.

    In the first model, two strains of rainbow trout (Onchorhynchus mykiss) divergent in stress-induced release of cortisol were investigated. This was done by observing behaviour and stress responses under different conditions. These strains were found to have divergent stress coping strategies based on the observed behaviour and levels of plasma cortisol. This divergence in behaviour could be associated with the CRH system, since the mRNA levels of CRH differed between the strains during stress. However, no differences between strains were observed in AVT or its receptor expressions.

    In the second model, non-selected rainbow trout were paired and the effect of intracerebroventricular (icv) injections of an active substance (AVT, CRH or the CRH related peptide Urotensin-I (UI)) on fights for dominance was investigated. One fish of the pair received the active substance icv and the other received saline icv. Fish receiving AVT became subordinate in accordance with the suggestion that AVT attenuates aggression in territorial vertebrates. Fish receiving CRH became subordinate whereas UI showed no effect on fights for dominance. Further, both CRH and UI induced an anxiety-related behaviour similar to non-ambulatory motor activity in rats. In addition, CRH appeared to affect the dopaminergic and serotonergic systems.

    In this thesis, it is suggested that CRH is involved in the behavioural modulation of the stress coping strategies in teleost fish. Further, AVT and CRH seem to act inhibitory on aggressive behaviour.

    List of papers
    1. Divergence in behavioural responses to stress in two strains of rainbow trout (Onchorhyncus mykiss) with contrasting stress responsiveness
    Open this publication in new window or tab >>Divergence in behavioural responses to stress in two strains of rainbow trout (Onchorhyncus mykiss) with contrasting stress responsiveness
    Show others...
    2005 (English)In: Hormones and Behavior, Vol. 48, no 5, p. 537-544Article in journal (Refereed) Published
    Identifiers
    urn:nbn:se:uu:diva-96792 (URN)
    Available from: 2008-02-21 Created: 2008-02-21 Last updated: 2009-04-05Bibliographically approved
    2. Effects of stress on AVT and CRH mRNA expression in two strains of rainbow trout (Onchorhynchus mykiss) divergent in stress response
    Open this publication in new window or tab >>Effects of stress on AVT and CRH mRNA expression in two strains of rainbow trout (Onchorhynchus mykiss) divergent in stress response
    Show others...
    (English)Article in journal (Refereed) Submitted
    Identifiers
    urn:nbn:se:uu:diva-96793 (URN)
    Available from: 2008-02-21 Created: 2008-02-21 Last updated: 2009-04-05Bibliographically approved
    3. Arginine-vasotocin influence on aggressive behaviour and dominance in rainbow trout
    Open this publication in new window or tab >>Arginine-vasotocin influence on aggressive behaviour and dominance in rainbow trout
    2009 (English)In: Physiology and Behavior, ISSN 0031-9384, E-ISSN 1873-507X, Vol. 96, no 3, p. 470-475Article in journal (Refereed) Published
    Abstract [en]

    The mammalian neuropeptide arginine-vasopressin (AVP) and its non-mammalian homologue arginine-vasotocin (AVT) are involved in social behavior including aggression in vertebrates. Juvenile rainbow trout (Onchorhynchus mykiss) are highly territorial and we tested the effect of two different doses each of AVT and Manning compound, an AVP V1A receptor antagonist, on aggressive behavior and the outcome of staged fights for social dominance in size matched pairs. Each pair consisted of one fish receiving AVT or Manning compound through an intracerebroventricular (icv) injection and one fish receiving a similar icv injection of saline. In addition, plasma cortisol and brain serotonergic activity were monitored. Fish receiving AVT at a dose of 200 ng became subordinate whereas AVT at a dose of 20 ng had no effect on the outcome of fights for social dominance. Further, Manning compound had no effect on the outcome of fights for dominance but at a dose of 200 ng it had an effect on fight duration. Fish receiving 200 ng Manning compound and losing dyadic fights appeared to take longer to become subordinate. Neither AVT nor Manning compound had any effects on plasma cortisol levels or brainstem serotonergic activity. The apparent inhibiting effect of AVT on aggression could be centrally mediated.

    Keywords
    Aggressive behavior, Arginine-vasopressin (AVP), Arginine-vasotocin (AVT), Cortisol, Dominance/subordinance, Dyadic interaction, Serotonin (5-hydroxytryptamine, 5-HT), Social system, Teleost fish
    National Category
    Biological Sciences
    Identifiers
    urn:nbn:se:uu:diva-96794 (URN)10.1016/j.physbeh.2008.11.013 (DOI)000263659800012 ()19087884 (PubMedID)
    Available from: 2008-02-21 Created: 2008-02-21 Last updated: 2017-12-14Bibliographically approved
    4. CRH and Urotensin-I effects on aggressive behaviour and anxiety in rainbow trout
    Open this publication in new window or tab >>CRH and Urotensin-I effects on aggressive behaviour and anxiety in rainbow trout
    (English)Article in journal (Refereed) Submitted
    Identifiers
    urn:nbn:se:uu:diva-96795 (URN)
    Available from: 2008-02-21 Created: 2008-02-21 Last updated: 2009-04-05Bibliographically approved
  • 10.
    Backström, Tobias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Pettersson, Andreas
    Johansson, Viktoria
    Winberg, Svante
    CRH and Urotensin-I effects on aggressive behaviour and anxiety in rainbow troutArticle in journal (Refereed)
  • 11.
    Backström, Tobias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Schjolden, Joachim
    Øverli, Øyvind
    Thörnqvist, Per-Ove
    Winberg, Svante
    Effects of stress on AVT and CRH mRNA expression in two strains of rainbow trout (Onchorhynchus mykiss) divergent in stress responseArticle in journal (Refereed)
  • 12.
    Backström, Tobias
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Winberg, Svante
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Arginine-vasotocin influence on aggressive behaviour and dominance in rainbow trout2009In: Physiology and Behavior, ISSN 0031-9384, E-ISSN 1873-507X, Vol. 96, no 3, p. 470-475Article in journal (Refereed)
    Abstract [en]

    The mammalian neuropeptide arginine-vasopressin (AVP) and its non-mammalian homologue arginine-vasotocin (AVT) are involved in social behavior including aggression in vertebrates. Juvenile rainbow trout (Onchorhynchus mykiss) are highly territorial and we tested the effect of two different doses each of AVT and Manning compound, an AVP V1A receptor antagonist, on aggressive behavior and the outcome of staged fights for social dominance in size matched pairs. Each pair consisted of one fish receiving AVT or Manning compound through an intracerebroventricular (icv) injection and one fish receiving a similar icv injection of saline. In addition, plasma cortisol and brain serotonergic activity were monitored. Fish receiving AVT at a dose of 200 ng became subordinate whereas AVT at a dose of 20 ng had no effect on the outcome of fights for social dominance. Further, Manning compound had no effect on the outcome of fights for dominance but at a dose of 200 ng it had an effect on fight duration. Fish receiving 200 ng Manning compound and losing dyadic fights appeared to take longer to become subordinate. Neither AVT nor Manning compound had any effects on plasma cortisol levels or brainstem serotonergic activity. The apparent inhibiting effect of AVT on aggression could be centrally mediated.

  • 13. Ballesteros, I.
    et al.
    Martin, M.P.
    Cerenius, Lage
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Telleria, M.T.
    Dieguez-Uribeondo, J.
    Lack of specificity of the molecular diagnosis method for identification of Aphanomyces astaci2007In: BFPP-CONNAISSANCE ET GESTION DU PATRIMOINE AQUATIQUE, ISSN 1297-6318, no 385, p. 17-24Article in journal (Refereed)
    Abstract [en]

    A recent PCR-test developed for identification of Aphanomyces astaci, the organism responsible for crayfish plague, provided false positives for Aphanomyces frigidophilus, Aphanomyces repetans, and some Saprolegnia spp. Real-time PCR showed that with the designed primers, A. astaci and A. frigidophilus cannot be distinguished. The results of this study show that this particular crayfish plague PCR-test ought to be improved and that molecular-based techniques need to be contrasted to histological evidences and disease history.

  • 14.
    Bangyeekhun, E
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Cerenius, Lage
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Molecular cloning and characterization of two serine proteinase genes from the crayfish plague fungus, Aphanomyces astaci2001In: Journal of Invertebrate Pathology, ISSN 0022-2011, E-ISSN 1096-0805, Vol. 77, no 3, p. 206-216Article in journal (Refereed)
    Abstract [en]

     

    Two novel genes encoding the serine proteinases, subtilisin (AaSP1) and trypsin (AaSP2), from Aphanomyces astaci were identified. Based on the amino acidconsensus sequences around the catalytic triad of these serine proteinases, degenerated oligonucleotides were designed for isolation of serine proteinase genes from a genomic DNA library. The AaSP1 gene encodes a full-length protein of 515 amino acids as a large precursor of 56 kDa. After cleavage of a predicted leader sequence of 18 residues and a prepeptide of 133 amino acids, the mature enzyme of 364 amino acids is generated with a calculated molecular mass of 39 kDa and a pI of 6.0. The primary sequence of AaSP1 showed similarity to both bacterial subtilisin and fungal subtilisin-like serine proteinases. Southern blot analysis of AaSP1 revealed the presence of at least two subtilisin genes in the A. astaci genome. Northern blot analysis indicated that the size of AaSP1 transcript was 1.6 kb. The AaSP2 gene encodes a prepropeptide of 276 amino acids with a molecular mass of 29 kDa. A mature protein of 237 amino acids is probably generated after cleavage of a 17-residue signal peptide and a 21-amino-acid prepeptide with a predicted molecular mass of 25 kDa and a pI of 6.0. The primary sequence of AaSP2 showed similarity to trypsin enzymes from various organisms. Southern blot analysis revealed the presence of multiple trypsin genes in the A. astaci genome. Northern blot analysis indicated that the size of AaSP2 transcript was 1.0 kb. The regulation of AaSP2 transcription was not controlled by nitrogen catabolic repression. However, the expression of AaSP2 was found to be specifically induced by crayfish plasma, implying a role in pathogenesis toward the crayfish host.

  • 15.
    Bangyeekhun, E
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Ryynänen, H J
    Henttonen, P
    Huner, J V
    Cerenius, Lage
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Sequence analysis of the ribosomal internal transcribed spacer DNA of the crayfish parasite Psorospermium haeckeli.2001In: Diseases of Aquatic Organisms, ISSN 0177-5103, E-ISSN 1616-1580, Vol. 46, no 3, p. 217-22Article in journal (Refereed)
    Abstract [en]

    Two morphotypes of the crayfish parasite Psorospermium haeckeli were isolated from 2 crayfish species of different geographical origin. The oval-shaped sporocysts were obtained from the epidermal and connective tissue beneath the carapace of the noble crayfish Astacus astacus fromSweden and Finland. Elongated spores were isolated from the abdominal muscle tissue of the red swamp crayfish Procambarus clarkii from USA. To compare genetic divergence of 2 morphotypes of the parasite, the ribosomal internal transcribed spacer (ITS) DNA (ITS 1 and ITS 2) and the 5.8S rRNAgene were cloned and sequenced. The analysed region is variable in length, with the ribosomal ITS sequence of the European morphotype longer than the North American one. Sequence diversity is found mainly in ITS 1 and ITS 2 regions, and there is 66% and 58% similarity between the 2 morphotypes,respectively. Thus, analysis of the ribosomal ITS DNA suggests that P. haeckeli forms obtained from Europe and North America are genetically diverse, which supports the previously reported morphological characteristics.

  • 16. Bangyeekhun, Eakaphun
    et al.
    Pylkkä, Päivi
    Vennerström, Pia
    Kuronen, Henry
    Cerenius, Lage
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. jämförande fysiologi.
    Prevalence of a single fish-pathogenic Saprolegnia sp. clone in Finland and Sweden.2003In: Dis Aquat Organ, ISSN 0177-5103, Vol. 53, no 1, p. 47-53Article in journal (Refereed)
  • 17. Barracco, M A
    et al.
    Söderhäll, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Effect of fixed epimastigote forms of Trypanosoma cruzi on the hemocytes and the prophenoloxidase-activating system of the crayfish Pacifastacus leniusculus.1996In: Braz J Med Biol Res, ISSN 0100-879X, Vol. 29, no 10, p. 1321-7Article in journal (Refereed)
  • 18. Barracco, MA
    et al.
    Soderhall, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Effect of fixed epimastigote forms of Trypanosoma cruzi on the hemocytes and the prophenoloxidase-activating system of the crayfish Pacifastacus leniusculus1996In: BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, ISSN 0100-879X, Vol. 29, no 10, p. 1321-1327Article in journal (Refereed)
    Abstract [en]

    The effect of the parasite Trypanosoma cruzi on the hemocytes and the prophenoloxidase (proPO)-activating system of the crayfish Pacifastacus leniusculus was studied. Incubation of the crayfish hemocyte lysate with fixed epimastigote forms of the parasite

  • 19. Bell, Alison M.
    et al.
    Backström, Tobias
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Huntingford, Felicity A.
    Pottinger, Tom G.
    Winberg, Svante
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
    Variable neuroendocrine responses to ecologically-relevant challenges in sticklebacks2007In: Physiology and Behavior, ISSN 0031-9384, E-ISSN 1873-507X, Vol. 91, no 1, p. 15-25Article in journal (Refereed)
    Abstract [en]

    Variable neuroendocrine responses to ecologically-relevant challenges in sticklebacks. PHYSIOL BEHAV 00(0) 000-000, 2006. Here, we compare the behavioral, endocrine and neuroendocrine responses of individual sticklebacks exposed to either an unfamiliar conspecific or to a predator. We found that the two stressors elicited a similar hypothalamic–pituitary–interrenal response as assessed by whole-body concentrations of cortisol, but produced quite different patterns of change in brain monoamine and monoamine metabolite content as assessed by concentrations of serotonin (5-HT), dopamine (DA), norepinephrine (NE) and the monoamine metabolites 5-hydroxyindole acetic acid (5-HIAA), homovanillic acid (HVA) and 3-4-dihydroxyphenylacetic acid (DOPAC). For example, relative to baseline levels, NE levels were elevated in individuals exposed to a predator but were lower in individuals confronted by a challenging conspecific. Levels of monoamine neurotransmitters in specific regions of the brain showed extremely close links with behavioral characteristics. Frequency of attacking a conspecific and inspecting a predator were both positively correlated with concentrations of NE. However, whereas serotonin was negatively correlated with frequency of attacking a conspecific, it was positively associated with predator inspection. The data indicate that the qualitative and quantitative nature of the neuroendocrine stress response of sticklebacks varies according to the nature of the stressor, and that interindividual variation in behavioural responses to challenge are reflected by neuroendocrine differences.

  • 20. Bidla, Gawa
    et al.
    Dushay, Mitchell S.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Theopold, Ulrich
    Crystal cell rupture after injury in Drosophila requires the JNK pathway, small GTPases and the TNF homolog eiger2007In: Journal of Cell Science, ISSN 0021-9533, E-ISSN 1477-9137, Vol. 120, no 7, p. 1209-1215Article in journal (Refereed)
    Abstract [en]

    The prophenoloxidase-activating cascade is a key component of arthropod immunity. Drosophila prophenoloxidase is stored in crystal cells, a specialized class of blood cells from which it is released through cell rupture. Within minutes after bleeding, prophenoloxidase is activated leading to visible melanization of the clot matrix. Using crystal cell rupture and melanization as readouts to screen mutants in signal transduction pathways, we show that prophenoloxidase release requires Jun N-terminal kinase, small Rho GTPases and Eiger, the Drosophila homolog of tumor necrosis factor. We also provide evidence that in addition to microbial products, endogenous signals from dying hemocytes contribute to triggering and/or assembly of the prophenoloxidase-activating cascade, and that this process can be inhibited in vitro and in vivo using the viral apoptotic inhibitor p35. Our results provide a more comprehensive view of immune signal transduction pathways, with implications for immune reactions where cell death is used as a terminal mode of cell activation.

  • 21. Bidla, Gawa
    et al.
    Hauling, Thomas
    Dushay, Mitchell S.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Theopold, Ulrich
    Activation of Insect Phenoloxidase after Injury: Endogenous versus Foreign Elicitors2009In: Journal Of Innate Immunity, ISSN 1662-811X, Vol. 1, no 4, p. 301-308Article in journal (Refereed)
    Abstract [en]

    The enzyme phenoloxidase (PO) is one of the first immune molecules that was identified in invertebrates. Recently, the immune function of PO has been challenged. We tested how PO is activated following injury in 2 insects, i.e. the fruit fly Drosophila melanogaster and the wax moth Galleria mellonella. Rapid PO activation in Drosophila was limited to discrete areas of the hemolymph clot which forms after injury. Surprisingly, unlike systemic PO activation during bacterial sepsis, clot melanization was not sensitive to microbial elicitors in our assay. Instead, Drosophila clot melanization was activated by endogenous signals such as apoptotic cells and was superinduced by phosphatidylserine, a negatively charged phospholipid normally found on the inner surface of the plasma membrane and exposed during apoptosis. In contrast, melanization in G. mellonella hemolymph was stronger and more uniform and was sensitive to peptidoglycan. This shows that both exogenous and endogenous signals can trigger the same immune mechanism in species and context-dependent ways. Our findings have implications for the evolutionary dynamics of immune mechanisms and are in agreement with recent comparisons of insect immune transcriptomes. Copyright (C) 2008 S. Karger AG, Basel

  • 22.
    Brelin, Daniel
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande Fysiologi.
    Divergent Stress Coping Strategies in Brown trout (Salmo trutta)2006Licentiate thesis, monograph (Other scientific)
  • 23.
    Brelin, Daniel
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Stress Coping Strategies in Brown Trout (Salmo Trutta): Ecological Significance and Effects of Sea-Ranching2008Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Two distinct stress coping strategies, proactive and reactive, have been stated in various animal studies, each associated with a set of behavioural and physiological characteristics. In a given challenging situation, proactive animals show more aggression, a higher general activity and a predominant sympathetic reaction. In contrast, the reactive copers respond more with immobility and avoidance, and a predominant parasympathetic/hypothalamic activation. This divergence in coping has also been indicated in salmonid fish. Interestingly, many of the differences reported between sea-ranched and wild fish resembles characteristics that differentiate proactive and reactive copers. In the present thesis it is shown that individuals with divergent stress coping styles are identifiable in several brown trout (Salmo trutta) populations. Further, the results show that the distribution of individuals displaying these distinct stress coping strategies differs between populations. This strongly indicates that these traits are heritable and that the variation in selection regime in the native rivers influences these traits. In addition, the results show that populations with hatchery origin are biased towards having higher frequencies of trout displaying a proactive style than populations having wild origin. Also, even though the frequency of early sexual maturation, known as a viable alternative life history in salmonids, differs between populations of brown trout, no link between stress coping strategy and early sexual maturation were found. However, this thesis show that maternal contribution, in the form of egg size, is of major importance whether the progeny will sexually mature early and that it also might be of importance for stress coping strategy. Further, correlations of traits commonly associated with stress coping strategies and behavioural syndromes across context and over time is investigated. The results show that individuals with a strong sympathetic reactivity are more prone to change their behaviour than others.

    List of papers
    1. Divergent stress coping strategies in juvenile brown trout (Salmo trutta)
    Open this publication in new window or tab >>Divergent stress coping strategies in juvenile brown trout (Salmo trutta)
    2005 (English)In: Annals of New York Academy of Sciences, Trends in comparative endocrinology and neurobiology, Vol. 1040, p. 239-245Article in journal (Refereed) Published
    Identifiers
    urn:nbn:se:uu:diva-96939 (URN)
    Available from: 2008-03-28 Created: 2008-03-28 Last updated: 2009-04-05Bibliographically approved
    2. Frequency distribution of coping strategies in four populations of brown trout (Salmo trutta)
    Open this publication in new window or tab >>Frequency distribution of coping strategies in four populations of brown trout (Salmo trutta)
    Show others...
    2008 (English)In: Hormones and Behavior, ISSN 0018-506X, E-ISSN 1095-6867, Vol. 53, no 4, p. 546-556Article in journal (Refereed) Published
    Abstract [en]

    In a challenging situation some animals respond by active avoidance, aggression and an activation of the sympathetic nervous system whereas others respond by immobility, low levels of aggression and a predominant adrenocortical stress response. When consistent over time and across situations such inter-individual differences in behaviourul and physiological stress responses are referred to as stress coping strategies. In a previous study we reported the existence of two distinct stress coping strategies in a sea-ranched brown trout (Salmo trutta) population. Using the same method, we here show that four brown trout populations with different origin, but reared under identical conditions, differ in their endocrine stress response, behaviour during hypoxia and aggression. Further more, if individuals are classified as high- and low responsive based on post-stress blood plasma noradrenalin levels (indicator of sympathetic reactivity) the frequency distribution shows that populations with hatchery origin are biased towards having higher frequencies of high responsive individuals. However, the number of high responsive trout ranges from 14-48% in the different populations which shows that generally the frequency is biased towards lower levels of high responsive individuals. We discuss different frequency-dependent mechanisms that maintain multiple phenotypes in populations and speculate about differences in selection regime among the studied populations.

    Keywords
    Fish, Proactive, Reactive, Stress, Noradrenalin, Adrenalin, Cortisol, Hypoxia, Animal personality;, Behavioural syndrome
    National Category
    Biological Sciences
    Identifiers
    urn:nbn:se:uu:diva-96940 (URN)10.1016/j.yhbeh.2007.12.011 (DOI)000255100700006 ()18280474 (PubMedID)
    Available from: 2008-03-28 Created: 2008-03-28 Last updated: 2017-12-14Bibliographically approved
    3. Is precocious sexual maturation in brown trout (Salmo trutta) associated with a proactive coping style?
    Open this publication in new window or tab >>Is precocious sexual maturation in brown trout (Salmo trutta) associated with a proactive coping style?
    Show others...
    (English)Article in journal (Refereed) Submitted
    Identifiers
    urn:nbn:se:uu:diva-96941 (URN)
    Available from: 2008-03-28 Created: 2008-03-28 Last updated: 2009-04-05Bibliographically approved
    4. Intra individual variation in behavioural and physiological stress responses in brown trout (Salmo trutta)
    Open this publication in new window or tab >>Intra individual variation in behavioural and physiological stress responses in brown trout (Salmo trutta)
    (English)Article in journal (Refereed) Submitted
    Identifiers
    urn:nbn:se:uu:diva-96942 (URN)
    Available from: 2008-03-28 Created: 2008-03-28 Last updated: 2009-04-05Bibliographically approved
  • 24.
    Brelin, Daniel
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Petersson, Erik
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Evolution, Animal Ecology.
    Dannewitz, Johan
    Dahl, Jonas
    Winberg, Svante
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Frequency distribution of coping strategies in four populations of brown trout (Salmo trutta)2008In: Hormones and Behavior, ISSN 0018-506X, E-ISSN 1095-6867, Vol. 53, no 4, p. 546-556Article in journal (Refereed)
    Abstract [en]

    In a challenging situation some animals respond by active avoidance, aggression and an activation of the sympathetic nervous system whereas others respond by immobility, low levels of aggression and a predominant adrenocortical stress response. When consistent over time and across situations such inter-individual differences in behaviourul and physiological stress responses are referred to as stress coping strategies. In a previous study we reported the existence of two distinct stress coping strategies in a sea-ranched brown trout (Salmo trutta) population. Using the same method, we here show that four brown trout populations with different origin, but reared under identical conditions, differ in their endocrine stress response, behaviour during hypoxia and aggression. Further more, if individuals are classified as high- and low responsive based on post-stress blood plasma noradrenalin levels (indicator of sympathetic reactivity) the frequency distribution shows that populations with hatchery origin are biased towards having higher frequencies of high responsive individuals. However, the number of high responsive trout ranges from 14-48% in the different populations which shows that generally the frequency is biased towards lower levels of high responsive individuals. We discuss different frequency-dependent mechanisms that maintain multiple phenotypes in populations and speculate about differences in selection regime among the studied populations.

  • 25.
    Brelin, Daniel
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Petersson, Erik
    Ian, Mayer
    Dannewitz, Johan
    Dahl, Jonas
    Winberg, Svante
    Is precocious sexual maturation in brown trout (Salmo trutta) associated with a proactive coping style?Article in journal (Refereed)
  • 26.
    Brelin, Daniel
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Petersson, Erik
    Winberg, Svante
    Divergent stress coping strategies in juvenile brown trout (Salmo trutta)2005In: Annals of New York Academy of Sciences, Trends in comparative endocrinology and neurobiology, Vol. 1040, p. 239-245Article in journal (Refereed)
  • 27.
    Brelin, Daniel
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Department of Ecology and Evolution, Animal Ecology. jämförande fysiologi.
    Petersson, Erik
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Department of Ecology and Evolution, Animal Ecology. populationsbiologi.
    Winberg, Svante
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Department of Ecology and Evolution, Animal Ecology. jämförande fysiologi.
    Divergent stress coping styles in juvenile brown trout (Salmo trutta)2005In: ANNALS OF THE NEW YORK ACADEMY OF SCIENCES: TRENDS IN COMPARATIVE ENDOCRINOLOGY AND NEUROBIOLOGY, Vol. 1040, p. 239-245Article in journal (Refereed)
    Abstract [en]

    Two distinct stress coping styles, proactive and reactive, have been stated in various animal studies. This divergence in coping has also been indicated in salmonid fish. Here, we test the hypothesis that divergent stress coping styles are identifiable in a sea-ranched brown trout population. To that end, we used a series of tests on individual juvenile brown trout, with each test including a common key aspect of the two different coping styles. Using a clustering method (SAS: PROC FASTCLUS), two groups that clearly differed both in blood chemistry (noradrenalin and adrenalin levels) following confinement and in behavior during hypoxia were identified.

  • 28.
    Brelin, Daniel
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Petersson, Erik
    Winberg, Svante
    Intra individual variation in behavioural and physiological stress responses in brown trout (Salmo trutta)Article in journal (Refereed)
  • 29.
    Cerenius, Lage
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Aphanomyces astaci (Crayfish plague)2006In: Atlas of European Crayfish Distribution and Diseases, Muséum National d'Histoire Naturelle, Paris, Patrimoines Naturels. , 2006Chapter in book (Refereed)
  • 30.
    Cerenius, Lage
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Babu, Ramesh
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Jiravanichpaisal, Pikul
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    In vitro effects on bacterial growth of phenoloxidase reaction products2010In: Journal of Invertebrate Pathology, ISSN 0022-2011, E-ISSN 1096-0805, Vol. 103, no 1, p. 21-23Article in journal (Refereed)
    Abstract [en]

    An active phenoloxidase preparation from the freshwater crayfish Pacifastacus leniusculus exhibited a strong antibacterial effect in vitro on the bacteria Aeromonas hydrophila, Escherichia coli, Streptococcus pneumoniae whereas a weaker but still significant effect against Bacillus cereus, Pseudomonas aeruginosa and Staphylococcus aureus. In most cases reduction of bacterial growth was stronger when dopamine was used as substrate as compared to L-dopa. The effect on bacteria was abolished if no substrate was available for the phenoloxidase or in the presence of the phenoloxidase inhibitor phenylthiourea.

  • 31.
    Cerenius, Lage
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology. Jämförande fysiologi.
    Bangyeekhun, Eakaphun
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology. Jämförande fysiologi.
    Keyser, Pia
    Söderhäll, Irene
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology. jämförande fysiologi.
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology. jämförande fysiologi.
    Host prophenoloxidase expression in freshwater crayfish is linked to increased resistance to the crayfish plague fungus, Aphanomyces astaci.2003In: Cell Microbiol, ISSN 1462-5814, Vol. 5, no 5, p. 353-7Article in journal (Refereed)
  • 32.
    Cerenius, Lage
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology.
    Haipeng, Liu
    State Key Laboratory of Marine Environmental Science, College of Oceanography and Environmental Science, Xiamen University, Xiamen, 361005 Fujian, China.
    Zhang, Yanjiao
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Rimphanitchayakit, Vichien
    Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
    Tassanakajon, Anchalee
    Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
    Andersson, M. Gunnar
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, The Linnaeus Centre for Bioinformatics.
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology.
    Söderhäll, Irene
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology.
    High sequence variability among hemocyte-specific Kazal-type proteinase inhibitors in decapod crustaceans2010In: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 34, no 1, p. 69-75Article in journal (Refereed)
    Abstract [en]

    Crustacean hemocytes were found to produce a large number of transcripts coding for Kazal-type proteinase inhibitors (KPIs). A detailed study performed with the crayfish Pacifastacus leniusculus and the shrimp Penaeus monodon revealed the presence of at least 26 and 20 different Kazal domains from the hemocyte KPIs, respectively. Comparisons with KPIs from other taxa indicate that the sequences of these domains evolve rapidly. A few conserved positions, e.g. six invariant cysteines were present in all domain sequences whereas the position of P1 amino acid, a determinant for substrate specificity, varied highly. A study with a single crayfish animal suggested that even at the individual level considerable sequence variability among hemocyte KPIs produced exist. Expression analysis of four crayfish KPI transcripts in hematopoietic tissue cells and different hemocyte types suggest that some of these KPIs are likely to be involved in hematopoiesis or hemocyte release as they were produced in particular hemocyte types or maturation stages only.

  • 33.
    Cerenius, Lage
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Laurent, P
    Edsman, L
    Protection of natives in a plague situation.2002In: Bulletin Francais de la Peche et de la Pisciculture, ISSN 0767-2861, Vol. 367, p. 909-910Article in journal (Refereed)
  • 34.
    Cerenius, Lage
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Lee, Bok Luel
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    The proPO-system: pros and cons for its role in invertebrate immunity2008In: Trends in immunology, ISSN 1471-4906, E-ISSN 1471-4981, Vol. 29, no 6, p. 263-271Article, review/survey (Refereed)
    Abstract [en]

    Melanisation is an important immune response in many invertebrates. Recent evidence also strongly implies that the melanisation (prophenoloxidase activating) cascade is intimately associated with the appearance of factors stimulating cellular defence by aiding phagocytosis and encapsulation reactions. However, some controversy exists in the field, and at least in flies and mosquitoes, the successful combat of some pathogens does not seem to be dependent on phenoloxidase activity. This may be because of redundancy among separate immune mechanisms, inappropriate testing, species differences or a combination thereof. Recently, by using RNA interference against phenoloxidase or in specific host-pathogen interactions where the pathogen prevents melanin production by the host, convincing data have confirmed the importance of this cascade in invertebrate innate immunity.

  • 35.
    Cerenius, Lage
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    CRUSTACEAN IMMUNITY AND COMPLEMENT - A PREMATURE COMPARISON1995In: American Zoologist, ISSN 0003-1569, E-ISSN 2162-4445, Vol. 35, no 1, p. 60-67Article in journal (Refereed)
    Abstract [en]

    The prophenoloxidase activating system constitutes a system for recognition of foreignness in several invertebrates. The system has been especially well studied in crustaceans and it will now be possible to begin structural comparisons between components

  • 36.
    Cerenius, Lage
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Söderhäll, Kenneth
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Isolation of pattern recognition molecules from crustaceans1995In: Techniques in Fish Immunology 4, 155-160, 1995Chapter in book (Refereed)
  • 37.
    Cerenius, Lage
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Saprolegniaceae; zoospore formation, virulence and pathogenesis in animal hosts.1996In: Biology of lower fungi, 1996, p. 97-116Chapter in book (Refereed)
  • 38.
    Cerenius, Lage
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Söderhäll, Kenneth
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    The prophenoloxidase-activating system in invertebrates.2004In: Immunol Rev, ISSN 0105-2896, Vol. 198, p. 116-26Article in journal (Refereed)
  • 39. Chacinska, Agnieszka
    et al.
    Lind, Maria
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    Frazier, Ann E
    Dudek, Jan
    Meisinger, Chris
    Geissler, Andreas
    Sickmann, Albert
    Meyer, Helmut E
    Truscott, Kaye N
    Guiard, Bernard
    Pfanner, Nikolaus
    Rehling, Peter
    Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17.2005In: Cell, ISSN 0092-8674, Vol. 120, no 6, p. 817-29Article in journal (Refereed)
  • 40. Chaga, O
    et al.
    Lignell, M
    Söderhäll, K
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Jämförande fysiologi.
    The haemopoietic cells of the freshwater crayfish Pacifastacus leniusculus1995In: Animal Biology, Vol. 4, p. 59-70Article in journal (Refereed)
  • 41. Cubitt, K. Fiona
    et al.
    Winberg, Svante
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Huntingford, Felicity A.
    Kadri, Sunil
    Crampton, Vivian O.
    Overli, Oyvind
    Social hierarchies, growth and brain serotonin metabolism in Atlantic salmon (Salmo salar) kept under commercial rearing conditions2008In: Physiology and Behavior, ISSN 0031-9384, E-ISSN 1873-507X, Vol. 94, no 4, p. 529-535Article in journal (Refereed)
    Abstract [en]

    Monitoring social interactions between individuals in large, high-density groups poses several challenges. Here we demonstrate that relative concentrations of serotonin (5-Hydroxytryptamine, 5-HT) and its principal catabolite 5-Hydroxyindoleacetic acid (5-HIAA) in brain tissue of individual fish reflect social organisation in large groups of farmed Atlantic salmon. In the central nervous system of vertebrates, the monoamine neurotransmitter/neuromodulator 5-HT is critical for maintaining adaptive physiological, cognitive and emotional processes. In both teleost fish and mammals it has previously been shown that social interactions in small groups lead to elevated 5-HT release and/or metabolism in subordinate individuals, as indicated by the 5-HIAA/5-HT concentration ratio. In the current study, evidence is presented of non-linear dominance hierarchies in farmed Atlantic salmon (Salmo salar) kept at high rearing densities. The physiological effect of these hierarchies was decreased when food resources were abundant, although some subordinate fish still showed altered brain serotonergic activity and failed to grow even feed was available in excess. The largest effect of decreased feed rations was seen in fish of intermediate size, where competition appeared to increase with reduced access to feed. The largest individuals in each rearing unit showed low 5-HIAA/5-HT ratios independent of feeding regime. A novel observation, with respect to previous studies, was that elevated brain 5-HIAA/5-HT ratios resulted from decreased 5-HT concentrations rather than elevated 5-HIAA in small fish. Thus, in light of the serotonin deficit hypothesis of depression, it cannot be excluded that social stress is important for animal welfare even in large, relatively homogenous groups of animals reared in captivity.

  • 42. Dieguez-Uribeondo, Javier
    et al.
    Cerenius, Lage
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    The inhibition of extracellular proteinases from Aphanomyces spp by three different proteinase inhibitors from crayfish blood1998In: Mycological Research, ISSN 0953-7562, E-ISSN 1469-8102, Vol. 102, no 7, p. 820-824Article in journal (Refereed)
    Abstract [en]

    Three different proteinase inhibitors purified from crayfish blood, a 23 kDa inhibitor of subtilisin, a 155 kDa trypsin-inhibitor (pacifastin) and an alpha(2)-macroglobulin were tested for their inhibitory activities against extracellular proteinases from

  • 43. Dieguez-Uribeondo, Javier
    et al.
    Cerenius, Lage
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Dykova, I.
    Gelder, S.R.
    Hentonen, P.
    Jiravanichpaisal, Pikul
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Lom, J.
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Pathogens, parasites and ectocommensals.2006In: Atlas of crayfish in Europe: Distribution and Diseases., Publications Scientifiques du Muséum National d'Histoire Naturelle Vol. 64 “Patrimoines Naturels” , 2006, p. 135-155Chapter in book (Other (popular science, discussion, etc.))
  • 44. Diéguez-Uribeondo, Javier
    et al.
    Fregeneda-Grandes, Juan M.
    Cerenius, Lage
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Pérez-Iniesta, Elena
    Aller-Gancedo, José Miguel
    Tellería, M. Teresa
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Martin, María P.
    Re-evaluation of the enigmatic species complex Saprolegnia diclina-Saprolegnia parasitica based on morphological, physiological and molecular data2007In: Fungal Genetics and Biology, ISSN 1087-1845, E-ISSN 1096-0937, Vol. 44, no 7, p. 585-601Article in journal (Refereed)
    Abstract [en]

    The phylogenetic relationships among isolates of the Saprolegnia diclina-Saprolegnia parasitica complex were investigated based on ITS rDNA sequences, and correlated with morphological and physiological characters. The isolates studied belong to five phylogenetically separate clades. The majority of presumed parasitic isolates, mostly isolated from fish lesions, fell within a clade that comprises isolates which has been variously named as S. diclina Type 1, S. parasitica, Saprolegnia salmonis or just as unnamed Saprolegnia sp. Presence of bundles of long-hooked hairs on secondary cysts, high frequency of retracted germination, and oogonia production at 7 degrees C (when occurring) were characteristic of this clade. A single isolate identified as S. diclina Type 2 clustered in a clade along with Saprolegnia ferax isolates. The isolates identified as S. diclina s. str. (S. diclina Type 3) distributed in two clades and appeared closely related to Saprolegnia multispora and to a number of Chilean isolates identified as Saprolegnia australis. The ITS sequences of clade I were almost identical even though the isolates were of diverse geographical origins and showed physiological and morphological differences and variations in their pathogenicity. This suggest these species reproduces clonally even in apparently sexually competent isolates. Adaptation to parasitism in Saprolegnia might have occurred at spore level by the development of long-hooked hairs to facilitate host attachment and selection of a retracting germination. The use of the name S. parasitica should be assigned to isolates of clade I that contained isolates forming cysts with bundles of long-hooked hairs.

  • 45. Diéguez-Uribeondo, Javier
    et al.
    García, Miguel A
    Cerenius, Lage
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Kozubíková, Eva
    Ballesteros, Isabel
    Windels, Carol
    Weiland, John
    Kator, Howard
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Martín, María P
    Phylogenetic relationships among plant and animal parasites, and saprotrophs in Aphanomyces (Oomycetes)2009In: Fungal Genetics and Biology, ISSN 1087-1845, E-ISSN 1096-0937, Vol. 46, no 5, p. 365-376Article in journal (Refereed)
    Abstract [en]

    Molecular phylogenetic relationships among 12 species of Aphanomyces de Bary (Oomycetes) were analyzed based on 108 ITS sequences of nuclear rDNA. Sequences used in the analyses belonged to the major species currently available in pure culture and GenBank. Bayesian, maximum likelihood, and maximum parsimony analyses support that Aphanomyces constitutes a monophyletic group. Three independent lineages were found: (i) plant parasitic, (ii) animal parasitic, and (iii) saprotrophic or opportunistic parasitic. Sexual reproduction appeared to be critical in plant parasites for survival in soil environments while asexual reproduction seemed to be advantageous for exploiting specialization in animal parasitism. Repeated zoospore emergence seems to be an advantageous property for both plant and animal parasitic modes of life. Growth in unspecific media was generally faster in saprotrophs compared with parasitic species. A number of strains and GenBank sequences were found to be misidentified. It was confirmed molecularly that Aphanomyces piscicida and Aphanomyces invadans appear to be conspecific, and found that Aphanomyces iridis and Aphanomyces euteiches are closely related, if not the same, species. This study has shown a clear evolutionary separation between Aphanomyces species that are plant parasites and those that parasitize animals. Saprotrophic or opportunistic species formed a separate evolutionary lineage except Aphanomyces stellatus whose evolutionary position has not yet been resolved.

  • 46.
    Diéguez-Uribeondo, Javier
    et al.
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Huang, TS
    Cerenius, Lage
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Söderhäll, Kenneth
    Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Physiological adaptation of an Aphanomyces astaci strain isolated from the freshwater crayfish Procambarus clarkii 1995In: Mycological Research, ISSN 0953-7562, E-ISSN 1469-8102, Vol. 99, no 5, p. 574-578Article in journal (Refereed)
    Abstract [en]

    Physiological, epidemiological and genetical properties of an Aphanomyces astaci strain (Pc) isolated from the warm water crayfish, Procambarus clarkii, were compared to other A. astaci strains isolated from the cold water crayfish Astacus astacus,

  • 47.
    Donpudsa, Suchao
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology.
    Söderhäll, Irene
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology.
    Rimphanitchayakit, Vichien
    Cerenius, Lage
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology.
    Tassanakajon, Anchalee
    Söderhäll, Kenneth
    Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
    Proteinase inhibitory activities of two two-domain Kazal proteinase inhibitors from the freshwater crayfish Pacifastacus leniusculus and the importance of the P2 position in proteinase inhibitory activity2010In: Fish and Shellfish Immunology, ISSN 1050-4648, E-ISSN 1095-9947, Vol. 29, no 5, p. 716-723Article in journal (Refereed)
    Abstract [en]