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2022 (Engelska)Ingår i: New Biotechnology, ISSN 1871-6784, E-ISSN 1876-4347, Vol. 71, s. 21-29Artikel i tidskrift (Refereegranskat) Published
Abstract [en]
Aiming to accommodate the unmet need for easily accessible biomarkers with a focus on biological differences between haematological diseases, the diagnostic value of plasma proteins in acute leukaemias and lymphomas was investigated. A multiplex proximity extension assay (PEA) was used to analyze 183 proteins in diagnostic plasma samples from 251 acute leukaemia and lymphoma patients and compared with samples from 60 healthy controls. Multivariate modelling using partial least square discriminant analysis revealed highly significant differences between distinct disease subgroups and controls. The model allowed explicit distinction between leukaemia and lymphoma, with few patients misclassified. Acute leukaemia samples had higher levels of proteins associated with haemostasis, inflammation, cell differentiation and cell-matrix integration, whereas lymphoma samples demonstrated higher levels of proteins known to be associated with tumour microenvironment and lymphoma dissemination. PEA technology can be used to screen for large number of plasma protein biomarkers in low mu L sample volumes, enabling the distinction between controls, acute leukaemias and lymphomas. Plasma protein profiling could help gain insights into the pathophysiology of acute leukaemia and lymphoma and the technique may be a valuable tool in the diagnosis of these diseases.
Ort, förlag, år, upplaga, sidor
ElsevierElsevier BV, 2022
Nyckelord
Acute leukaemia, Lymphoma, Plasma protein biomarker, Tumor microenvironment, Proximity extension assay
Nationell ämneskategori
Hematologi
Identifikatorer
urn:nbn:se:uu:diva-481929 (URN)10.1016/j.nbt.2022.06.005 (DOI)000830815200001 ()35779858 (PubMedID)
Forskningsfinansiär
CancerfondenVetenskapsrådet
2022-08-192022-08-192024-01-15Bibliografiskt granskad