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  • 1.
    Andersson, Staffan
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Fysikundervisningens didaktik.
    Andersson Chronholm, Jannika
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Fysikundervisningens didaktik.
    Gelin, Birgitta
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Avhopp och genomströmning: Hur ser det ut och vad kan man göra?2012Ingår i: Universitetspedagogisk utveckling och kvalitet – i praktiken! / [ed] Geir Gunnlaugsson, Uppsala: Uppsala universitet, 2012, s. 127-146Kapitel i bok, del av antologi (Refereegranskat)
    Abstract [sv]

    Vi har studerat programgenomströmning och faktorer som påverkar den för ingenjörsutbildningar vid Uppsala universitet. Analys av studiestatistik visar att antalet studenter som avbryter utbildningarna är störst i början, men att det relativa avhoppet är förhållandevis konstant till och med femte terminen. En kvalitativ analys av enkätsvar om studieval och avhoppsöverväganden identifierade fem områden av särskild betydelse för studenters relation till sin utbildning: intresse, mål, framgång, trivsel och kvalitet. Studien ger en värdefull grund för fortsatt utvecklingsarbete för att förbättra genomströmning, både på ingenjörsutbildningar och på andra program.

  • 2. Baguma, Yona
    et al.
    Sun, Chuanxin
    Ahlandsberg, Staffan
    Mutisya, Joel
    Palmqvist, Sara
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Rubaihayo, Patrick R.
    Magambo, Michael J.
    Egwang, Thomas G.
    Larsson, HÃ¥kan
    Jansson, Christer
    Expression patterns of the gene encoding starch branching enzyme II in the storage roots of cassava (Manihot esculenta Crantz)2003Ingår i: Plant Science, ISSN 0168-9452, E-ISSN 1873-2259, Vol. 164, nr 5, s. 833-839Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Spatial and temporal expression patterns of the sbeII and sbeI genes, encoding starch branching enzyme II and I, respectively, in cassava (Manihot esculenta Crantz) were studied at different phenological stages of the crop. A partial cDNA for sbeII in cassava was cloned and used along with a cDNA-specific fragment of sbeI. As the cassava plant aged, the transcriptional activity of the sbeII and sbeI genes in the underground storage roots increased, whereas the activity in other organs remained the same or declined. At 180 days after planting (d.a.p.), levels of sbeII and sbeI transcripts in storage roots were very low, whereas at 360 d.a.p., the levels had increased dramatically. The 360 d.a.p. old storage roots also accumulated gbssII and gbssI transcripts, as well as a longer gbssI transcript, gbssI′. The difference between the gbssI and gbssI′ transcripts was shown to be due to differential splicing, whereby the gbssI′ transcript retained the first three introns. Unexpectedly, expression of sbeII and sbeI in the 360 d.a.p. storage roots exhibited fluctuations during the 24 h cycle, both under the normal light/dark regime and under continuous light or continuous dark conditions.

  • 3.
    Blom, Mattias Bolkéus
    et al.
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Magnusson, LarsUppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Ekonomisk-historiska institutionen.
    Samhällsvetenskapliga fakulteten vid Uppsala universitet 50 år, 1964–20142014Samlingsverk (redaktörskap) (Övrig (populärvetenskap, debatt, mm))
  • 4. Dell, Holger
    et al.
    Husfeldt, Thore
    Marx, Daniel
    Taslaman, Nina
    Wahlén, Martin
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Exponential Time Complexity of the Permanent and the Tutte Polynomial2014Ingår i: ACM Transactions on Algorithms, ISSN 1549-6325, E-ISSN 1549-6333, Vol. 10, nr 4, s. 21-Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    We show conditional lower bounds for well-studied #P-hard problems: -The number of satisfying assignments of a 2-CNF formula with n variables cannot be computed in time exp(o(n)), and the same is true for computing the number of all independent sets in an n-vertex graph. -The permanent of an n x n matrix with entries 0 and 1 cannot be computed in time exp(o(n)). -The Tutte polynomial of an n-vertex multigraph cannot be computed in time exp(o(n)) at most evaluation points (x, y) in the case of multigraphs, and it cannot be computed in time exp(o(n/poly log n)) in the case of simple graphs. Our lower bounds are relative to (variants of) the Exponential Time Hypothesis (ETH), which says that the satisfiability of n-variable 3-CNF formulas cannot be decided in time exp(o(n)). We relax this hypothesis by introducing its counting version #ETH; namely, that the satisfying assignments cannot be counted in time exp(o(n)). In order to use #ETH for our lower bounds, we transfer the sparsification lemma for d-CNF formulas to the counting setting.

  • 5. Edqvist, Johan
    et al.
    Rönnberg, Elin
    Rosenquist, Sara
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Blomqvist, Kristina
    Viitanen, Lenita
    Salminen, Tiina A
    Nylund, Matts
    Tuuf, Jessica
    Mattjus, Peter
    Plants express a lipid transfer protein with high similarity to mammalian sterol carrier protein-2.2004Ingår i: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 279, nr 51, s. 53544-53Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    This is the first report describing the cloning and characterization of sterol carrier protein-2 (SCP-2) from plants. Arabidopsis thaliana SCP-2 (AtSCP-2) consists of 123 amino acids with a molecular mass of 13.6 kDa. AtSCP-2 shows 35% identity and 56% similarity to the human SCP-2-like domain present in the human D-bifunctional protein (DBP) and 30% identity and 54% similarity to the human SCP-2 encoded by SCP-X. The presented structural models of apo-AtSCP-2 and the ligand-bound conformation of AtSCP-2 reveal remarkable similarity with two of the structurally known SCP-2s, the SCP-2-like domain of human DBP and the rabbit SCP-2, correspondingly. The AtSCP-2 models in both forms have a similar hydrophobic ligand-binding tunnel, which is extremely suitable for lipid binding. AtSCP-2 showed in vitro transfer activity of BODIPY-phosphatidylcholine (BODIPY-PC) from donor membranes to acceptor membranes. The transfer of BODIPY-PC was almost completely inhibited after addition of 1-palmitoyl 2-oleoyl phosphatidylcholine or ergosterol. Dimyristoyl phosphatidic acid, stigmasterol, steryl glucoside, and cholesterol showed a moderate to marginal ability to lower the BODIPY-PC transfer rate, and the single chain palmitic acid and stearoyl-coenzyme A did not affect transfer at all. Expression analysis showed that AtSCP-2 mRNA is accumulating in most plant tissues. Plasmids carrying fusion genes between green fluorescent protein and AtSCP-2 were transformed with particle bombardment to onion epidermal cells. The results from analyzing the transformants indicate that AtSCP-2 is localized to peroxisomes.

  • 6. Hammargren, Jenni
    et al.
    Rosenquist, Sara
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Jansson, Christer
    Knorpp, Carina
    A novel connection between nucleotide and carbohydrate metabolism in mitochondria: sugar regulation of the Arabidopsis nucleoside diphosphate kinase 3a gene2008Ingår i: Plant Cell Reports, ISSN 0721-7714, E-ISSN 1432-203X, Vol. 27, nr 3, s. 529-534Artikel i tidskrift (Refereegranskat)
  • 7.
    Hertting, Nils
    et al.
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Statsvetenskapliga institutionen.
    Jungerhem, Sven
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Sjöblom, Arne
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Företagsekonomiska institutionen.
    Kvalitetsbedömning av effektivitetsrevisionens granskningsprocess (2012)2012Rapport (Övrigt vetenskapligt)
  • 8.
    Kassman Rudolphi, Åsa
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Tillämpad materialvetenskap.
    Orvehed, Maria
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Woldegiorgis, Sara
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Ingenjörsutbildningar vid Uppsala universitet. Studenter granskar några utbildningar ur genusperspektiv2008Rapport (Övrig (populärvetenskap, debatt, mm))
    Abstract [sv]

    Från september 2007 till maj 2008 genomfördes ett projekt på Teknisk-naturvetenskapliga fakulteten vid Uppsala universitet, där studenter granskade några tekniska utbildningar ur genusperspektiv.Syftet med granskningen var att upptäcka eventuella strukturer som skulle kunna utgöra hinder för kvinnliga studenter, samt att få en kunskapsbas för ett förbättringsarbete.

    De utbildningar som ingick var civilingenjörsprogrammen i Teknisk fysik, System i teknik och samhälle och Kemiteknik, vilka utgör tre av totalt nio civilingenjörsprogram och har olika andel kvinnliga studenter. Avsikten var att även granska ett av tre högskoleingenjörsprogram vid fakulteten, men programmet utgick i brist på granskare.

    Totalt deltog femton studenter från årskurserna två och tre, varav åtta kvinnliga studenter och sju manliga, som granskare. De granskade föreläsningar, lektioner, laborationer och seminarier, och tittade även på studiemiljön, och kvinnliga och manliga förebilder. Bland lärare och handledare såg granskarna prov på både genusmedveten pedagogik och besvärande stereotypa könsuppfattningar. Bristen på kvinnliga förebilder vad gäller föreläsare, lektionsledare,handledare och författare till kurslitteratur var stor. It/datorer och deras användande upplevdes vara en manlig domän. Laborationer utmärktes av att studenterna fritt valde grupper och då oftast jobbade i könsuppdelade grupper och med samma personer hela tiden. Möjligen fick kvinnliga studenter mer hjälp på gott och ont. Ett par av granskarnas förbättringsförslag, utöver fler kvinnliga förebilder, var obligatorisk utbildning i genusmedveten pedagogik för alla lärare, mer aktiverande undervisningsformer, en genusutbildning för alla studenter och återkommande uppföljningar av dessa aktiviteter.

    Granskarna rapporterade också om tysta studenter och låg grad av interaktion och aktiverande pedagogik. Utöver granskningsresultaten och förslagen på förbättringar, har projektet lett till en ökad genusmedvetenhet hos de deltagande studenterna. Metoden att använda studenter som granskare har fungerat bra och kan prövas inom fler områden. Projektet har också gett projektgruppen en ökad insikt i studenternas vardag.

  • 9. Mutisya, Joel
    et al.
    Sathish, P.
    Sun, Chuanxin
    Andersson, Lena
    Ahlandsberg, Staffan
    Baguma, Yona
    Palmqvist, Sara
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Odhiambo, Benjamin
    Ņman, Per
    Jansson, Christer
    Starch branching enzymes in sorghum (Sorghum bicolor) and barley (Hordeum vulgare): Comparative analyses of enzyme structure and gene expression2003Ingår i: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 160, nr 8, s. 921-930Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Summary A genomic clone for starch branching enzyme (SBE) IIb was isolated from a sorghum bacterial artificial chromosome (BAC) library. The promoter and 5′ flanking sequence, the first four exons and introns as well as the last exon and the 3′ untranslated region were sequenced. The tentative transcription start site of sorghum sbeIIb was mapped based on alignment with the maize sbeIIb gene. The exon-intron structure of the 5′ portion of sorghum sbeIIb was similar to that of maize sbeIIb but differed from that of barley sbeIIb. Specifically, the intronic Bbl element involved in the endosperm specific expression of barley sbeIIb was lacking in the sorghum gene. A cDNA clone for sorghum sbeIIb was reverse PCR amplified and found to encode an 803 amino acids long protein. The amino acid sequence of sorghum SBEIIb was compared to that of sorghum SBEI and corresponding enzymes in barley. The overall identity in amino acid sequence was 54 percnt; in the central portion of the enzymes. A major difference between the SBEII and SBEI isoforms was a 67 amino acids-long C-terminal extension in the SBEIs. The spatial and temporal expression patterns of sorghum sbeIIb was determined and compared with those of the sorghum gene for SBEI and the barley genes for SBEIIB and SBEI. All four genes exhibited a seed specific expression. However, while barley sbeIIb and sbeI transcripts were detected exclusively in the endosperm, the sorghum genes were expressed also in the embryo. The activity of sorghum sbeIIb and sbeI exhibited a late onset, with a peak of transcription at around 22 days after pollination. This is similar to the pattern of barley sbeI but different from that of barley sbeIIb, which showed a peak of transcription at 12 days after pollination.

  • 10. Mutisya, Joel
    et al.
    Sun, Chuanxin
    Palmqvist, Sara
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Baguma, Yona
    Odhiambo, Benjamin
    Jansson, Christer
    Transcriptional regulation of the sbeIIb genes in sorghum (Sorghum bicolor) and barley (Hordeum vulgare): Importance of the barley sbeIIb second intron2006Ingår i: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 163, nr 7, s. 770-780Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Summary The transcriptional activity of the sorghum sbeIIb gene, encoding starch branching enzyme IIb, is seed specific, with expression in both the endosperm and the embryo. In comparison, expression of barley sbeIIb is confined to the endosperm, whereas that of barley sbeIIa occurs in endosperm, embryonic and vegetative tissues. It has been suggested that the second intron of barley sbeIIb may be instrumental in conferring endosperm-specific expression. Therefore, to further investigate the regulatory mechanisms of barley and sorghum sbe, we examined the tissue-specific activity of the sorghum sbe promoter in transient assays of green fluorescent protein (gfp) reporter constructs. We found that, when linked to the barley sbeIIb second intron, the sorghum sbeIIb promoter could not drive gfp transcription in sorghum or barley embryonic cells. Similar results were obtained for the barley sbeIIa promoter. Database searches showed that sequences homologous to the barley sbeIIb intron also exist in introns and flanking regions of some other grass genes. Deletion mutagenesis of the sorghum sbeIIb promoter identified the minimal promoters required for high- and low-level expression, respectively, but did not reveal any putative promoter elements crucial for expression. A sequence with similarity to the SURE element, implicated in sugar signaling, was located in the distal promoter region of sorghum sbeIIb, upstream of the minimal promoters. SURE elements are present in the proximal promoter regions of the sugar-regulated barley iso1 gene, and barley sbeIIb. In keeping in line with these observations, RNA-gel blot analyses demonstrated that expression of barley sbeIIb was sugar inducible, whereas that of sorghum sbeIIb was not.

  • 11. Mutisya, Joel
    et al.
    Sun, Chuanxin
    Palmqvist, Sara
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Baguma, Yona
    Odhiambo, Benjamin
    Jansson, Christer
    Transcriptional regulation of the sbeIIb genes in sorghum (Sorghum bicolor) and barley (Hordeum vulgare): Importance of the barley sbeIIb second intron2006Ingår i: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 163, nr 7, s. 770-780Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Summary The transcriptional activity of the sorghum sbeIIb gene, encoding starch branching enzyme IIb, is seed specific, with expression in both the endosperm and the embryo. In comparison, expression of barley sbeIIb is confined to the endosperm, whereas that of barley sbeIIa occurs in endosperm, embryonic and vegetative tissues. It has been suggested that the second intron of barley sbeIIb may be instrumental in conferring endosperm-specific expression. Therefore, to further investigate the regulatory mechanisms of barley and sorghum sbe, we examined the tissue-specific activity of the sorghum sbe promoter in transient assays of green fluorescent protein (gfp) reporter constructs. We found that, when linked to the barley sbeIIb second intron, the sorghum sbeIIb promoter could not drive gfp transcription in sorghum or barley embryonic cells. Similar results were obtained for the barley sbeIIa promoter. Database searches showed that sequences homologous to the barley sbeIIb intron also exist in introns and flanking regions of some other grass genes. Deletion mutagenesis of the sorghum sbeIIb promoter identified the minimal promoters required for high- and low-level expression, respectively, but did not reveal any putative promoter elements crucial for expression. A sequence with similarity to the SURE element, implicated in sugar signaling, was located in the distal promoter region of sorghum sbeIIb, upstream of the minimal promoters. SURE elements are present in the proximal promoter regions of the sugar-regulated barley iso1 gene, and barley sbeIIb. In keeping in line with these observations, RNA-gel blot analyses demonstrated that expression of barley sbeIIb was sugar inducible, whereas that of sorghum sbeIIb was not.

  • 12. Mutisya, Joel
    et al.
    Sun, Chuanxin
    Rosenquist, Sara
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Baguma, Yona
    Jansson, Christer
    Diurnal oscillation of SBE expression in sorghum endosperm2009Ingår i: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 166, nr 4, s. 428-434Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Summary Spatial and temporal expression patterns of the sorghum SBEI, SBEIIA and SBEIIB genes, encoding, respectively, starch branching enzyme (SBE) I, IIA and IIB, in the developing endosperm of sorghum (Sorghum bicolor) were studied. Full-length genomic and cDNA clones for sorghum were cloned, and the SBEIIA cDNA was used together with gene-specific probes for sorghum SBEIIB and SBEI. In contrast to sorghum SBEIIB, which was expressed primarily in endosperm and embryo, SBEIIA was also expressed in vegetative tissues. All three genes shared a similar temporal expression profile during endosperm development, with a maximum activity at 15–24 d after pollination. This differed from barley and maize, in which SBEI gene activity showed a significantly later onset compared to that of SBEIIA and SBEIIB. Expression of the three SBE genes in the sorghum endosperm exhibited a diurnal rhythm during a 24-h cycle.

  • 13.
    Nordgren, Eva
    et al.
    Uppsala universitet, Universitetsbiblioteket.
    Gerén, Linda
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Student and library services in cooperation2013Konferensbidrag (Övrig (populärvetenskap, debatt, mm))
    Abstract [en]

    Abstract

    At Ångström Laboratory, one of the largest campuses at Uppsala University, the Library and the Student Services Office merged in November 2012. This merger is a pilot project to improve service for students and faculty. Ångström Laboratory has around 900 staff and almost 10,000 students, of whom most also spend time at other campuses. In this paper we describe the background and the implementation of the pilot project.

    One of the main reasons for moving together was the wish to gather together all kinds of student services, including the distribution of written examinations in one place. The central location and open environment of the Library made it a good choice.

    The heart of the Library and Student Services is an open office consisting of two service desks located in the library area. There are silent study areas; computers for searching and printing, an area for relaxing with newspapers and journals, meeting rooms and offices for the staff. The result is a lively place with a cosy atmosphere.

    As the Library and the Student Services still belong to different parts of the University we are now starting to find out how best to collaborate. To understand more we log all the questions we get and the services we deliver. We also have meetings together and use the same lunch room to get to know each other and our different functions. We will define which matters can be solved in common, and how we can back each other up when necessary.

  • 14. Rintala, Ulla
    et al.
    Kairamo, Anna-Kaarina
    Andersson, Staffan
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Fysiska sektionen, Institutionen för fysik och astronomi, Fysikundervisningens didaktik.
    Strandås, Magnus
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Kelly, Kevin
    Goncalves, lsabel
    Lucas, Ana
    Tabacco, Anita
    Knowing our students - Different approaches to student retention: Experiences of the ATTRACT project2012Ingår i: SEFI 2012 Conference Proceedings: Engineering Education 2020:  Meet the Future, Thessaloniki, Greece, 2012Konferensbidrag (Refereegranskat)
  • 15.
    Rydbeck, Kerstin
    et al.
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Historisk-filosofiska fakulteten, Institutionen för ABM.
    Eriksson Lindvall, CarinRügheimer, LouiseUppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Head over Heels: Seventeen Women Researchers' Thoughts on Shoes: A collection of essays2014Samlingsverk (redaktörskap) (Övrig (populärvetenskap, debatt, mm))
  • 16.
    Sjöblom, Arne
    et al.
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Företagsekonomiska institutionen.
    Jungerhem, Sven
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Hertting, Nils
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Samhällsvetenskapliga fakulteten, Institutet för bostads- och urbanforskning (IBF).
    Öberg, Sture
    Larsson, Mats
    Kvalitetsbedömning av effektivitetsrevisionens granskningsrapporter: Pilotstudie 20102010Rapport (Övrigt vetenskapligt)
  • 17.
    Sun, Chuanxin
    et al.
    Sveriges Lantbruksuniversitet.
    Palmqvist, Sara
    Uppsala universitet, Universitetsförvaltningen, Områdeskanslier.
    Olsson, Helena
    Borén, Mats
    Ahlandsberg, Staffan
    Jansson, Christer
    A Novel WRKY Transcription Factor, SUSIBA2, Participates in Sugar Signaling in Barley by Binding to the Sugar-Responsive Elements of the iso1 Promoter2003Ingår i: The Plant Cell, Vol. 15, nr 9, s. 2076-2092Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    SURE (sugar responsive) is a cis element in plant sugar signaling. The SURE element was reported first for potato, in which it confers sugar responsiveness to the patatin promoter. A SURE binding transcription factor has not been isolated. We have isolated a transcription factor cDNA from barley and purified the corresponding protein. The transcription factor, SUSIBA2 (sugar signaling in barley), belongs to the WRKY proteins and was shown to bind to SURE and W-box elements but not to the SP8a element in the iso1 promoter. Nuclear localization of SUSIBA2 was demonstrated in a transient assay system with a SUSIBA2:green fluorescent protein fusion protein. Exploiting the novel transcription factor oligodeoxynucleotide decoy strategy with transformed barley endosperm provided experimental evidence for the importance of the SURE elements in iso1 transcription. Antibodies against SUSIBA2 were produced, and the expression pattern for susiba2 was determined at the RNA and protein levels. It was found that susiba2 is expressed in endosperm but not in leaves. Transcription of susiba2 is sugar inducible, and ectopic susiba2 expression was obtained in sugar-treated leaves. Likewise, binding to SURE elements was observed for nuclear extracts from sugar-treated but not from control barley leaves. The temporal expression of susiba2 in barley endosperm followed that of iso1 and endogenous sucrose levels, with a peak at similar to12 days after pollination. Our data indicate that SUSIBA2 binds to the SURE elements in the barley iso1 promoter as an activator. Furthermore, they show that SUSIBA2 is a regulatory transcription factor in starch synthesis and demonstrate the involvement of a WRKY protein in carbohydrate anabolism. Orthologs to SUSIBA2 were isolated from rice and wheat endosperm.; SURE (sugar responsive) is a cis element in plant sugar signaling. The SURE element was reported first for potato, in which it confers sugar responsiveness to the patatin promoter. A SURE binding transcription factor has not been isolated. We have isolated a transcription factor cDNA from barley and purified the corresponding protein. The transcription factor, SUSIBA2 (sugar signaling in barley), belongs to the WRKY proteins and was shown to bind to SURE and W-box elements but not to the SP8a element in the iso1 promoter. Nuclear localization of SUSIBA2 was demonstrated in a transient assay system with a SUSIBA2:green fluorescent protein fusion protein. Exploiting the novel transcription factor oligodeoxynucleotide decoy strategy with transformed barley endosperm provided experimental evidence for the importance of the SURE elements in iso1 transcription. Antibodies against SUSIBA2 were produced, and the expression pattern for susiba2 was determined at the RNA and protein levels. It was found that susiba2 is expressed in endosperm but not in leaves. Transcription of susiba2 is sugar inducible, and ectopic susiba2 expression was obtained in sugar-treated leaves. Likewise, binding to SURE elements was observed for nuclear extracts from sugar-treated but not from control barley leaves. The temporal expression of susiba2 in barley endosperm followed that of iso1 and endogenous sucrose levels, with a peak at approximately 12 days after pollination. Our data indicate that SUSIBA2 binds to the SURE elements in the barley iso1 promoter as an activator. Furthermore, they show that SUSIBA2 is a regulatory transcription factor in starch synthesis and demonstrate the involvement of a WRKY protein in carbohydrate anabolism. Orthologs to SUSIBA2 were isolated from rice and wheat endosperm.; SURE (sugar responsive) is a cis element in plant sugar signaling. The SURE element was reported first for potato, in which it confers sugar responsiveness to the patatin promoter. A SURE binding transcription factor has not been isolated. We have isolated a transcription factor cDNA from barley and purified the corresponding protein. The transcription factor, SUSIBA2 (sugar signaling in barley), belongs to the WRKY proteins and was shown to bind to SURE and W-box elements but not to the SP8a element in the iso1 promoter. Nuclear localization of SUSIBA2 was demonstrated in a transient assay system with a SUSIBA2:green fluorescent protein fusion protein. Exploiting the novel transcription factor oligodeoxynucleotide decoy strategy with transformed barley endosperm provided experimental evidence for the importance of the SURE elements in iso1 transcription. Antibodies against SUSIBA2 were produced, and the expression pattern for susiba2 was determined at the RNA and protein levels. It was found that susiba2 is expressed in endosperm but not in leaves. Transcription of susiba2 is sugar inducible, and ectopic susiba2 expression was obtained in sugar-treated leaves. Likewise, binding to SURE elements was observed for nuclear extracts from sugar-treated but not from control barley leaves. The temporal expression of susiba2 in barley endosperm followed that of iso1 and endogenous sucrose levels, with a peak at similar to 12 days after pollination. Our data indicate that SUSIBA2 binds to the SURE elements in the barley iso1 promoter as an activator. Furthermore, they show that SUSIBA2 is a regulatory transcription factor in starch synthesis and demonstrate the involvement of a WRKY protein in carbohydrate anabolism. Orthologs to SUSIBA2 were isolated from rice and wheat endosperm.; SURE (sugar responsive) is a cis element in plant sugar signaling. The SURE element was reported first for potato, in which it confers sugar responsiveness to the patatin promoter. A SURE binding transcription factor has not been isolated. We have isolated a transcription factor cDNA from barley and purified the corresponding protein. The transcription factor, SUSIBA2 (sugar signaling in barley), belongs to the WRKY proteins and was shown to bind to SURE and W-box elements but not to the SP8a element in the iso1 promoter. Nuclear localization of SUSIBA2 was demonstrated in a transient assay system with a SUSIBA2:green fluorescent protein fusion protein. Exploiting the novel transcription factor oligodeoxynucleotide decoy strategy with transformed barley endosperm provided experimental evidence for the importance of the SURE elements in iso1 transcription. Antibodies against SUSIBA2 were produced, and the expression pattern for susiba2 was determined at the RNA and protein levels. It was found that susiba2 is expressed in endosperm but not in leaves. Transcription of susiba2 is sugar inducible, and ectopic susiba2 expression was obtained in sugar-treated leaves. Likewise, binding to SURE elements was observed for nuclear extracts from sugar-treated but not from control barley leaves. The temporal expression of susiba2 in barley endosperm followed that of iso1 and endogenous sucrose levels, with a peak at ∌12 days after pollination. Our data indicate that SUSIBA2 binds to the SURE elements in the barley iso1 promoter as an activator. Furthermore, they show that SUSIBA2 is a regulatory transcription factor in starch synthesis and demonstrate the involvement of a WRKY protein in carbohydrate anabolism. Orthologs to SUSIBA2 were isolated from rice and wheat endosperm.

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