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Virus removal filtration constitutes an essential component in biopharmaceutical manufacturing processes for both recombinant and plasma-derived protein products. The increasing demand for protein-based therapies necessitates expedited analytical screening methodologies for virus filtration materials and process parameters utilizing high-throughput development (HTPD) platforms. This study examines a novel HTPD methodology, specifically the implementation of analytical centrifugal ultrafiltration, as a scaled-down virus removal filtration tool. A nanocellulose-derived filter membrane of controlled porosity (33 & micro;m thickness, 23 nm) was integrated into a centrifugal ultrafiltration apparatus. The system underwent comprehensive evaluation to determine centrifugal ultrafiltration flux characteristics, product yield across varying buffer viscosities, virus removal capacity utilizing model MS2 (27 nm) and Phi X174 (28 nm) bacteriophages, and IgG throughput capacity. Application of centrifugal force facilitated the generation of multiple filtration pressure gradients suitable for small-volume sample processing. The resultant flux decay profiles exhibited high reproducibility, demonstrating superior IgG throughput and virus removal efficiency. Overall, the approach adds value to the arsenal of analytical tools useful for HTPD in biopharmaceutics.

The presented work investigates critical aspects of rational formulation design through machine learning (ML) methodology to identify essential patterns in immediate release ibuprofen oral dosage products influencing its pharmacokinetic profile. Registry data were extracted and standardized into a consistent format using pandas (v1.3.5) in Python 3.9, with special attention to variant nomenclature for identical excipients. Patterns regarding the usage of dissolution-modifying excipients as well as ibuprofen variants were used to investigate their influence on clinical pharmacokinetic profile. Film coated tablets emerged as the most common immediate release dosage form of ibuprofen utilizing ibuprofen acid as the active ingredient and sodium lauryl sulfate as surfactant/ wetting agent. Ibuprofen special variants, such as ibuprofen sodium dihydrate, ibuprofen lysine and ibuprofen arginine, offer more rapid drug release and onset with significantly reduced tmax and increased Cmax as well as generally lower bioavailability variance compared to standard immediate release ibuprofen oral dosage forms. The approaches presented in this article will be helpful in better understanding of rational formulation strategies and support regulatory scientific decisions ensuring predictable bioavailability and reproducible clinical responses.

A composition comprising trifluoroiodomethane (CF 3 I) and 1, 1-difluoroethylene (R-1132 a) is described. The composition may further comprise additional compounds, such as at least one non-flammable compound selected from the group consisting of: carbon dioxide (CO 2; R-744), tetrafluoromethane (R-14), trifluoromethane (R-23) and perfluoroethane (R-116), or at least one additional compound having a lower volatility than 1, 1-difluoroethylene selected from the group consisting of: 1, 2-trifluoroethylene (R-1123), difluoromethane (R-32), propane (R-290), propylene (R-1270), fluoroethane (R-161), pentafluoroethane (R-125), and 1, 2-tetrafluoroethane (R-134 a), 2, 3-tetrafluoropropene (R-1234 yf), isobutane (R-600 a) n-butane (R-600), trans-1, 3-tetrafluoropropene (R-1234 ze (E)) 3, 3-trifluoropropene (R-1243 zf), 1,2, 3-pentafluoropropene (R-1225 ye) 1,2, 3-heptafluoropropane (R-227 ea), 1-difluoroethane (R-152 a) 1,2, 3-heptafluoropropane (R-227 ea) 1, 1-difluoroethane (R-152 a). The composition is useful as a refrigerant in a vapor compression heat transfer system.

The challenge of using patient-specific, autologous stem cell therapies in clinical settings is the need for advanced cell processing and expansion technologies. These include decentralized, small-scale manufacturing at the point of care in hospitals. The highest risk for contamination in cell-based therapy products comes from animal- and human-derived components such as serum, blood components, and growth factors. To mitigate the risk of adventitious microorganism contamination, preventive measures like size-exclusion virus removal filtration of cell media components can be employed. This article examines the impact of nanofiltration using nanocellulose-based virus clearance filter paper on the differentiation of human pluripotent stem cells into insulin-producing pancreatic islets (SC-islets). The cells were monitored for biomarkers using flow cytometry and immunohistochemistry along the 7-stage differentiation protocol. The produced SC-islets were evaluated functionally using low and high glucose stimulation under dynamic perifusion conditions. Pluripotent stem cells grown in culture media filtered through 20 nm cut-off nanocellulose filters showed similar expression of desired biomarkers at each stage compared to the control group. At the end of stage 7, SC-islets exhibited a rounded shape and strong expression of insulin, glucagon, and somatostatin in both the control and filtered media groups. The present study demonstrates that SC-islets differentiated with nanofiltered media were functional.

In this study, systematic analysis of nanotechnology education programmes was carried out from a global and local perspective. Although several universities offer 3-4 year nanotechnology-centred educational programmes, in general the most common modes for nanotechnology education include either modular courses integrated in traditional scientific disciplines or 2-year specialised MSc programmes. Nanotechnology education has a number of peculiarities. For example, it is common to include “nano”-labelling of education for marketing purposes to attract students to study traditional disciplines. Another conclusion of the analysis is that specialised nanotechnology programmes require access to advanced characterisation equipment, which can be limiting for programmes with small number of students. The nanotechnology education in Sweden does not differ much from other countries in its structure. MSc programmes or modular courses in nanotechnology are offered by major universities. Lund University and KTH offer 2-year MSc programmes with specialisation in nanoscience, having strong focus on nanoelectronics and nanophysics, whereas Chalmers is offering nanoelectronics as a part of international 2-year Erasmus Mundus MSc programme in nanoscience and nanotechnology- EMMNano. The nanotechnology education at Uppsala University is conducted mainly through modular courses, with significantly higher number of courses specialised in life sciences applications than that at other major Swedish universities. At this time, it appears that no 2-year MSc programme is offered by major Swedish universities with specialisation in nanomedicine. Finally, extensive analysis of nanotechnology for life science education is conducted to highlight core concepts in the field.

The present work aims to provide a framework for teaching nanotechnology for life sciences applications, with special focus on nanotechnology-enabled treatments based on advanced physical and biological principles. By expanding and adapting the so-called “Big Ideas” of NSE to further include core elements of cell and molecular biology, an eight-level learning progression is suggested. The challenges of teaching nanotechnology are further addressed in different learning contexts to highlight the importance of size-dependent physical and biological interactions. A number of core nanomedicine concepts are discussed, including, e.g., directed self-assembly, enhance permeability and retention effect, protein corona, size-dependent cellular uptake, disease-specific environment, and theranostics. The outcome of this work will be useful for educators designing curriculum with specialization in nanomedicine.

This is a filtration membrane comprising cellulose fibers, where the membrane has a pore size distribution such that the modal pore diameter is between 10 nm and 25 nm and/or where less than 5% of the pore volume comprises pores larger than 40nm and which have a total porosity greater than 30%.

An X-ray amorphous magnesium carbonate is disclosed that is characterized by a cumulative pore volume of pores with a diameter smaller than 10 nm of at least 0.018 cm3/g, and a specific surface areas of at least 60 m2/g. The X-ray amorphous magnesium carbonate is produced by reacting an inorganic magnesium compound with alcohol in a CO2 atmosphere. The X-ray amorphous magnesium carbonate can be a powder or a pellet and acts as a desiccant in, for example, production of food, chemicals or pharmaceuticals.

A filtration membrane comprising cellulose fibres, the membrane having a pore size distribution such that the modal pore diameter is between 10 nm and 25 nm and/or wherein less than 5% of the pore volume comprises pores of greater than 40 nm and having a total porosity greater than 30%.