Logo: to the web site of Uppsala University

This study focuses on the fabrication and analysis of 3D-printed high-detail resolution cellulose acetate (CA) structures, particularly examining their specific surface area per volume (S_v). While electrospinning is a widely used technique for creating nanofiber membranes with high S_v, which is advantageous for applications like chromatography, the performance could be further improved by precisely controlling fiber placement. To further develop membranes, this research explores the use of electroprinting with small distances between nozzle and collector, here named near-collector electroprinting, to create 3D structures. By optimizing printing parameters, in particular the reduction of the nozzle-to-collector distance, 3D structures with precise fiber placement within a few micrometers were fabricated. The specific surface area per volume was calculated for both 3D-printed and electrospun filters. Results showed that 3D-printed structures with a 5 μm pitch achieved a S_v similar to electrospun filters.

Incorporating polyethyleneimine (PEI) in the CA ink enabled the 3D-printed structures to gain ion binding capacity which was further investigated. This ion-exchange ability which integrated into the printing step, eliminating the need for a separate post-modification process in bio-separation applications. By switching the substrate voltage from positive to negative, relative to the grounded nozzle, the printed fiber diameter decreased substantially for the CA ink with PEI. The S_v for near-collector electroprinted fibers of this material could therefore potentially be higher than that of electrospun membranes, provided that an order of magnitude higher printing speed, than presently possible can be used. These findings suggest that near-collector electroprinted CA structures offer potential improvements in membrane design and performance, making them a promising alternative to traditional electrospun membranes for bio-separation applications.

Electrospinning is a technique used to fabricate polymer fibers in micro- and nanoscales. Due to the large distance between the nozzle and collector, there is a limited positioning accuracy of electrospun fibers. To enhance the possibility of fabricating structures with micrometer placement, an electroprinting technique has been developed. By reducing the distance between the nozzle and the collector it is demonstrated that it is possible to get an improved control over fiber positioning which gives a possibility to fabricate designed 3D structures at the micron scale. In this study, cellulose acetate (CA) has been selected as a biomaterial to advance the 3D printing of membranes with possible use in separation applications. Various parameters, such as CA concentration and molecular weight, printing speed, printing pattern, applied voltage, etc. are evaluated with respect to printing control. Results indicate that by optimizing the printing parameters it is possible to print structures with inter- fiber distances down to 3 mu m and fiber diameters at a sub-mu m scale. This electroprinting development is promising for the fabrication of customized separation membranes. However, printing speed still remains a challenge.

3D printing is envisioned to play an important role in the production of membranes for e.g., water purification and bio-separation applications due to the prospect of creating new and cleverly designed structures. Among different 3D printing techniques, direct ink writing offers the opportunity to print a wide variety of materials with high-detail resolution. There is a range of parameters that need to be optimized in order to develop robust printing techniques at that scale. In this study, cellulose acetate (CA), which is a biocompatible material, has been used as an ink. In order to examine the printability and the possibility of printing features as small as a few mu m, nozzles with different diameters and inks with varying amounts and molecular weights of CA were investigated. Findings in this study indicate that, depending on the wetting on the underlaying structure, the nozzle's internal and external diameter affects the detail resolution of the printed structure. Different inks result in different widths of printed strands and generally a higher amount and higher molecular weights of CA results in higher detail resolution. However, too high amount of CA and molecular weight will increase the clogging risk in the nozzle. In this study, the internal size of the nozzle was 3 mu m, and by selecting a suitable ink, it was possible to print strands down to 1 mu m size and 6 mu m inter-strand distance in the air, bridging supports with limited sagging. Furthermore, wall structures consisting of 300 layers, corresponding to about 300 mu m in total height, were successfully printed.

Direct ink writing (DIW) is a promising additive manufacturing (AM) technique in the field of microsystems technology due to the potential for high detail resolution and the wide choice of materials suitable for the technique. In this study, inks of polyvinylidene fluoride-trifluoroethylene (PVDF-TrFE) as well as composite inks with reduced graphene oxide (PVDF-TrFE-rGO) were developed and adapted for continuous flow DIW. The composite PVDF-TrFE-rGO inks achieved percolation after 1.5 wt% and electrical conductivity of 2.8 S/cm at the highest loading investigate in this study (7 wt%). The inks were successfully printed with minimum nozzle diameter of 40 μm on different substrates including glass, metal and a nitrile elastomer. It was also demonstrated that the inks can be used to create a fully 3D-printed piezoelectric device with the predicted response, i.e. the fabrication technique did not deteriorate the functionality of the device. The conductive composite ink was successfully utilized as an effective electrode in the device. It was therefore demonstrated that by combining materials, such as the composite PVDF-TrFE-rGO ink and the co-polymer PVDF-TrFE with additive manufacturing techniques, the fabrication of low-cost, versatile devices can be achieved.

The graphene oxide (GO) microstructure, in terms of flake distribution, folding, and crumpling, in thin films affects properties such as electrical conductivity and optical transparency after GO reduction. A thin film can be tailored to the user's application if the microstructure resulting from different deposition methods can be controlled. In this work, we compare the microstructures of GO coatings created through electrospray deposition (ESD) with random deposition processes. The comparisons include both MATLAB simulations and a dip coating process. The microstructure of ESD GO thin films can be altered by changing the distance between the nozzle and the substrate. We developed a semi-automatic image analysis script that analyzes scanning electron microscopy images to find effects of GO stacking or agglomeration, without the risk of human bias. A low nozzle to substrate distance creates structures of flat GO flakes, but solvent flooding the samples causes drying patterns. A high nozzle to substrate distance causes folding and crumpling of the GO flakes due to solvent evaporation, resulting in agglomerated GO on the substrate. For our ESD setup, a nozzle to substrate distance of 2-4 mm produced GO coatings with the lowest combined influence of drying effects and GO flake folding or crumpling.

Two-photon polymerization (2PP) is an efficient technique to achieve high-resolution, three-dimensional (3D)-printed complex structures. However, it is restricted to photocurable monomer combinations, thus presenting constraints when aiming at attaining functionally active resist formulations and structures. In this context, metal nanoparticle (NP) integration as an additive can enable functionality and pave the way to more dedicated applications. Challenges lay on the maximum NP concentrations that can be incorporated into photocurable resist formulations due to the laser-triggered interactions, which primarily originate from laser scattering and absorption, as well as the limited dispersibility threshold. In this study, we propose an approach to address these two constraints by integrating metallic Rh NPs formed ex situ, purposely designed for this scope. The absence of surface plasmon resonance (SPR) within the visible and near-infrared spectra, coupled with the limited absorption value measured at the laser operating wavelength (780 nm), significantly limits the laser-induced interactions. Moreover, the dispersibility threshold is increased by engineering the NP surface to be compatible with the photocurable resin, permitting us to achieve concentrations of up to 2 wt %, which, to our knowledge, is significantly higher than the previously reported limit (or threshold) for embedded metal NPs. Another distinctive advantage of employing Rh NPs is their role as promising contrast agents for X-ray fluorescence (XRF) bioimaging. We demonstrated the presence of Rh NPs within the whole 2PP-printed structure and emphasized the potential use of NP-loaded 3D-printed nanostructures for medical devices.

Near-field electrospinning (NFES) is an additive manufacturing technique that allows for both high-resolution 3D structures and a wide variety of printed materials. Typically, a high electric field between a nozzle, the spinneret, and the substrate creates a mu m-sized jet of a supplied liquid material. With mm distances between spinneret and sample, it is possible to have a fair control of the lateral placement of the deposited material. The placement is, however, distributed by various electrostatic phenomena, and this is one of the present challenges in developing NFES into a more versatile technique. In this paper, a higher degree of control in NFES placement was achieved through manipulation of the electric field direction, using an auxiliary steering electrode. The position of a polycaprolactone plastic jet was determined in real-time with a camera attached to a stereo microscope. The measured position was used to calculate an applied potential to the steering electrode to guide the plastic jet to the desired position. The placement accuracy was measured both at the substrate and during flight using the camera and microscope. The higher control was revealed through the deposition of plastic fibers in a pattern with decreasing separation, with and without the active steering electrode enabled. It is in the authors' opinion that the fabrication of dense structures could be possible with further refinement of the technique.

Piezoelectric motors offer an attractive alternative to electromagnetic actuators in portable haptic interfaces: they are compact, have a high force-to-volume ratio, and can operate with limited or no gearing. However, the choice of a piezoelectric motor type is not obvious due to differences in performance characteristics. We present our evaluation of two commercial, operationally different, piezoelectric motors acting as actuators in two kinesthetic haptic grippers, a walking quasi-static motor and a traveling wave ultrasonic motor. We evaluate each gripper's ability to display common virtual objects including springs, dampers, and rigid walls, and conclude that the walking quasi-static motor is superior at low velocities. However, for applications where high velocity is required, traveling wave ultrasonic motors are a better option.