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Title [sv]
Intracellulär kinetik för enskilda molekyler - experimentella, teoretiska och beräkningsbaserade ansatser
Title [en]
Single molecule approaches to intracellular regulation combining experimental, theoretical and computational approaches
Abstract [sv]
A challenge in biochemistry today is to clarify the key differences between how an enzyme or transcription factor operates in a living cell as compared to in the test tube. For this purpose we have developed pioneering single molecule techniques for studying the function of macromolecules in living cells. These methods will make it possible to probe kinetics without synchronizing the population of molecules, which is usually not possible in vivo. Three different aspects of in vivo reaction kinetics will be clarified: 1. How do proteins find their substrates in living cells? 2. How can we accurately measure enzymatic activity in vivo? 3. How can enzymatic kinetics be correctly integrated in dynamics models for intracellular physiology? This program builds on my previously developed techniques for single molecule imaging in vivo (Science 2007) that are here combined with extensions of my previous theoretical models (e.g. Science 2003, Nature Physics 2009) and state-of-the-art computational approaches to stochastic reaction diffusion kinetics (e.g. PLoS CB 2006). The research program depends on deep level integration of in vivo single molecule techniques with computational and theoretical modeling. This is also what makes this research program unique. While the particular questions are specific in nature, the theoretical and experimental methods we will develop to address these questions will have far reaching and general applications.
Principal InvestigatorElf, Johan
Coordinating organisation
Uppsala University
Funder
Period
2010-01-01 － 2012-12-31
Identifiers
DiVA, id: project:3676Project, id: 2009-02725_VR

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