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Title [sv]
Polyelektrolytinduceerad segregation av proteiner
Title [en]
Polyelectrolyte-induced segregation of proteins
Abstract [sv]
We will investigate how the interaction between two different water soluble proteins is affected by the presence of a polyelectrolyte of opposite charge to both proteins, with special focus on segregation or proteins confined to the same polyelectrolyte network. By investigating the importance of the charge density of proteins and polyelectrolyte and other factors affecting the strength of electrostatic interactions the results will clarify to what extent electrostatic interactions alone can be responsible for segregation of two different proteins (P1 and P2). Another objective is to clarify to what extent intrinsic (short range) attractions, not mediated by the polyelectrolyte, can be responsible for segregation. The processes will be investigated on physiologically relevant length scales by means of confocal microscopy techniques, where we will take advantage of the possibilities offered by the use of micropipettes/micromanipulators to trigger and control processes in small compartments. The problems addressed are relevant for encapsulation of two proteins in microgels for protein drug delivery, but also related to the long studied but unsolved problem of protein ?sorting? in the secretory machinery of living cells, where charged biopolymers are known to play a role.
Principal InvestigatorHansson, Per
Coordinating organisation
Uppsala University
Funder
Period
2012-01-01 － 2014-12-31
National Category
Physical Chemistry
Identifiers
DiVA, id: project:4475Project, id: 2011-04325_VR

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