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Project

Project type/Form of grant
Project grant
Title [sv]
Genregleringens fysik i den växande cellen.
Title [en]
The physics of gene regulation in the context of the growing cell
Abstract [sv]
The main objective is to develop new technology for studying gene regulation at high spatial and temporal resolution in living cells. The methods will be used to answer fundamental questions about how gene regulation has evolved to meet the demands for accuracy and speed in the intracellular environment. The intracellular kinetics is elucidated by single-molecule methods (Elf et al. Science 2007, PNAS 2011) that make it possible to determine reaction rates also at steady state, i.e. without perturbing the system. The experimental studies are necessarily combined with the development of state-of-the-art tools for computational (Elf et al. PNAS 2010b) and theoretical (Elf et al. PNAS 2010a, Nature Physics 2009) analysis of the stochastic and spatially distributed aspects of intracellular processes. Specific questions are related to how the cell can keep repressor controlled genes repressed just after replication, what is the physical trade-off between fast search on non-specific DNA sequences and high probability of binding at specific DNA sequences, and which of the schemes that have been proposed for facilitating transcription factor search kinetics (sliding, intersegment transfer, looping, spatial position of the genes) are actually used in the living cell? The project is unique in its ambition to make a physically consistent description of transcription factor mediated gene regulation in living cells.
Principal InvestigatorElf, Johan
Coordinating organisation
Uppsala University
Funder
Period
2013-01-01 － 2017-12-31
National Category
Bioinformatics (Computational Biology)BiophysicsMicrobiology
Identifiers
DiVA, id: project:4869Project, id: 2012-04027_VR

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