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Title [sv]
singel-cell biokemi
Title [en]
single cell biochemistry
Abstract [en]
Methods to determine levels of protein-protein interactions are essential in analysis of cellular signaling events. Over the years a multitude of methods have been developed to facilitate this, one such method is the in situ proximity ligation assay (in situ PLA) that was developed in our laboratory. Although in situ PLA is a sensitive and selective method for detecting protein-protein interactions, it is not possible to determine how large proportion of the protein pool that is actually involved in an interaction. Information on levels of both free and interacting proteins are important to determine if the interactions monitored are a result of a post-transcriptional protein modification, e.g. phosphorylations or ubiquitination, that changes the conformation of the proteins and subsequently results in a different dissociation constant (KD), or if the amount of interactions reflects mere expression levels of the proteins. To be able to detect both the interacting and non-interacting protein pools simultaneously, we developed two methods that can visualize interactions between protein A and protein B, and at the same time detect the total amounts of protein A and protein B, respectively. A patent application covering the inventions has been filed and we herein want to develop the methods to a stage where they can be developed into products.
Principal InvestigatorSöderberg, Ola
Coordinating organisation
Uppsala University
Funder
Period
2017-01-01 － 2017-12-31
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
DiVA, id: project:5934Project, id: 2017-00653_VR
v. 2.47.0
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