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Title [sv]
Påvisande av enstaka patologiska celler
Title [en]
Detection of rare, pathological cells
Abstract [en]
My lab has a strong track record of inventing, developing, and of disseminating tools for molecular analysis in Sweden and internationally.With this project we aim to establish and provide a generally applicable high-performance toolbox for identifying rare, pathological cells via their DNA or RNA, of value in basic research and for liquid biopsy.Our strategy builds on a series of recently developed molecular reagents – gap-fill padlock probes, ExCirc probes, UnFold probes, sRCA, and RCA Reporters – that can be combined to achieve highly selective parallel analyses of individual molecular events to identify rare pathological cells in tissues or released to in the bloodstream.A common element in our molecular approaches is to capture target sequences in circular DNA strands that are replicated and then interrogated in a further powerful local amplification, allowing unique molecular events, and cells harboring these, to be identified with negligible risk of error and vastly expanded, for convenient digital detection above and beyond any nonspecific background.As has been the case for earlier successful technologies from my lab, we will make the tools to be developed herein available to Swedish scientists via the national infrastructure for molecular biosciences, SciLifeLab, as well as develop them as commercial products for research, drug development and for multimodal molecular diagnostics.
Principal InvestigatorLandegren, Ulf
Coordinating organisation
Uppsala University
Funder
Period
2019-01-01 － 2022-12-31
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
DiVA, id: project:6278Project, id: 2018-02943_VR
v. 2.49.0
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