Currently, permeation enhancers are used as excipients in oral drug formulations to aid drug absorption. It is likely that permeation enhancers regulate drug absorption by opening tight junction pores which explains the need for molecular-level understanding of tight junction pores. Claudins play a major role in the formation and regulation of epithelial tight junctions in human intestines. The general structure-function properties and the specific contributions of individual claudins were studied; however, their relationship with tight junction pores were not explored in detail. In this project, claudin structures predicted from alpha fold were used to construct a model of the tight junction complex with pores. The model needs further improvisation and was not detailed enough to demonstrate the relation between claudins and tight junction pores. By understanding such molecular level relations can help in the knowledge-based design of permeation enhancers that could potentially enhance drug absorption.