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Isolation and characterization of a highly competitive commensal Enterobacteriaceae from human stool by high-throughput screening and next generation sequencing
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för cell- och molekylärbiologi.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för cell- och molekylärbiologi.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för cell- och molekylärbiologi.
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(Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
Nationell ämneskategori
Naturvetenskap
Identifikatorer
URN: urn:nbn:se:uu:diva-487782OAI: oai:DiVA.org:uu-487782DiVA, id: diva2:1707910
Tillgänglig från: 2022-11-02 Skapad: 2022-11-02 Senast uppdaterad: 2022-11-02
Ingår i avhandling
1. To defend or to offend?: Bacterial defence and competition systems
Öppna denna publikation i ny flik eller fönster >>To defend or to offend?: Bacterial defence and competition systems
2022 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Bacteria are prokaryotic microorganisms that can be found in all niches of the biosphere. Bacteria must constantly evolve in order to survive and adapt to their environments. Defence systems and competition systems play a vital role in ensuring the survival of bacteria. The projects included in this thesis aim to further understand how these systems function, as well as adapting them in order to design probiotic strains with built-in biocontainment mechanisms. In paper I, we investigate how internal expression of Rearrangement hotspot (Rhs) toxins allows protection against Rhs toxin delivery in S. Typhimurium. In paper II, we examine bacterial competition systems found in enterotoxigenic E. coli (ETEC) strains and explore the evolution of toxin arsenals within ETEC family lineages. In paper III, we develop a fluorescence flow cytometry-based high-throughput screening method and apply it to isolate a highly competitive commensal E. coli strain that possesses a diverse arsenal of competition systems. In paper IV, we design and construct a synthetic CRISPR system that is able to protect E. coli from acquisition of antibiotic resistance genes through horizontal gene transfer. The work presented in this thesis contribute to our understanding of the functions of bacterial defence and competition systems, at the same time, laying the groundwork for how these systems can be studied in a high-throughput manner as well as how they can be adapted in the future to design synthetic microbial strains with clinical and environmental applications.

Ort, förlag, år, upplaga, sidor
Uppsala: Acta Universitatis Upsaliensis, 2022. s. 31
Serie
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 2217
Nyckelord
bacterial defence systems; bacterial competition systems; toxin-antitoxin systems; colicin; contact-dependent growth inhibition (CDI); type VI secretion system (T6SS); Rearrangement hotspot (Rhs) proteins; CRISPR; horizontal gene transfer; antibiotic resistance; Salmonella; Enterotoxigenic E. coli (ETEC); probiotics; synthetic biology.
Nationell ämneskategori
Naturvetenskap
Identifikatorer
urn:nbn:se:uu:diva-487784 (URN)978-91-513-1657-4 (ISBN)
Disputation
2022-12-20, Room A1:107, BMC, Husargatan 3, Uppsala, 13:00 (Engelska)
Opponent
Handledare
Tillgänglig från: 2022-11-28 Skapad: 2022-11-02 Senast uppdaterad: 2022-11-28

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