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HSP70 Inhibition Leads to the Activation of Proteasomal System under Mild Hyperthermia Conditions in Young and Senescent Fibroblasts
Marmara Univ, Dept Biochem, Sch Med, TR-34854 Istanbul, Turkey.;Acibadem Mehmet Ali Aydinlar Univ, Dept Mol Biol & Genet, TR-34752 Istanbul, Turkey..
Marmara Univ, Dept Biochem, Sch Med, TR-34854 Istanbul, Turkey.;Marmara Univ, Genet & Metab Dis Res & Invest Ctr, TR-34854 Istanbul, Turkey..
Marmara Univ, Dept Biochem, Sch Med, TR-34854 Istanbul, Turkey.;Univ Hlth Sci, Hlth Applicat & Res Ctr, Afyon, Turkey..
Marmara Univ, Dept Biochem, Sch Med, TR-34854 Istanbul, Turkey..
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2020 (English)In: Oxidative Medicine and Cellular Longevity, ISSN 1942-0900, E-ISSN 1942-0994, Vol. 2020, article id 9369524Article in journal (Refereed) Published
Abstract [en]

Aging has been characterized with the accumulation of oxidized proteins, as a consequence of progressive decline in proteostasis capacity. Among others, proteasomal system is an efficient protein turnover complex to avoid aggregation of oxidized proteins. Heat shock protein 70 (HSP70) is another critical player that is involved in some key processes including the correct folding of misfolded proteins and targeting aggregated proteins to the proteasome for rapid degradation. The aim of this study was to determine the role of proteasomal system and heat shock proteins to maintain proteome balance during replicative senescence in mild hyperthermia conditions. Our results demonstrated that HSP40/70 machinery is induced by mild hyperthermia conditions independent from senescence conditions. Since HSP70 is largely responsible for the rapidly inducible cell protection following hyperthermia, the activation of "heat shock response" resulted in the elevation of HSP40/70 expressions as well as the proteasome activity. Interestingly, when HSP70 expression was inhibited, increased proteasomal activation was shown to be responsive to mild hyperthermia. Since HSP70 is involved in various stress-related pathways such as oxidative and endoplasmic reticulum stress, depletion of HSP70 expression may induce proteasomal degradation to maintain proteome balance of the cell. Thus, our data suggests that in mild heat stress conditions, molecular chaperone HSP70 plays an important role to avoid protein oxidation and aggregation; however, activities of proteasomal system are induced when HSP70 expression is depleted.

Place, publisher, year, edition, pages
Hindawi Publishing Corporation, 2020. Vol. 2020, article id 9369524
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Biochemistry and Molecular Biology Cell Biology
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URN: urn:nbn:se:uu:diva-409850DOI: 10.1155/2020/9369524ISI: 000522431900002PubMedID: 32190179OAI: oai:DiVA.org:uu-409850DiVA, id: diva2:1432925
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Swedish Research Council, 2015-4870Available from: 2020-05-28 Created: 2020-05-28 Last updated: 2020-05-28Bibliographically approved

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Bergquist, Jonas

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