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The Effects of GC-Biased Gene Conversion on Patterns of Genetic Diversity among and across Butterfly Genomes
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Evolutionary Biology.ORCID iD: 0000-0002-0537-8219
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Evolutionary Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Evolutionary Biology.ORCID iD: 0000-0002-0961-8427
2021 (English)In: Genome Biology and Evolution, E-ISSN 1759-6653, Vol. 13, no 5, article id evab064Article in journal (Refereed) Published
Abstract [en]

Recombination reshuffles the alleles of a population through crossover and gene conversion. These mechanisms have considerable consequences on the evolution and maintenance of genetic diversity. Crossover, for example, can increase genetic diversity by breaking the linkage between selected and nearby neutral variants. Bias in favor of G or C alleles during gene conversion may instead promote the fixation of one allele over the other, thus decreasing diversity. Mutation bias from G or C to A and T opposes GC-biased gene conversion (gBGC). Less recognized is that these two processes may-when balanced-promote genetic diversity. Here, we investigate how gBGC and mutation bias shape genetic diversity patterns in wood white butterflies (Leptidea sp.). This constitutes the first in-depth investigation of gBGC in butterflies. Using 60 resequenced genomes from six populations of three species, we find substantial variation in the strength of gBGC across lineages. When modeling the balance of gBGC and mutation bias and comparing analytical results with empirical data, we reject gBGC as the main determinant of genetic diversity in these butterfly species. As alternatives, we consider linked selection and GC content. We find evidence that high values of both reduce diversity. We also show that the joint effects of gBGC and mutation bias can give rise to a diversity pattern which resembles the signature of linked selection. Consequently, gBGC should be considered when interpreting the effects of linked selection on levels of genetic diversity.

Place, publisher, year, edition, pages
Oxford University Press, 2021. Vol. 13, no 5, article id evab064
Keywords [en]
genetic diversity, GC-biased gene conversion, Lepidoptera, linked selection, mutation bias
National Category
Genetics and Genomics Evolutionary Biology
Identifiers
URN: urn:nbn:se:uu:diva-448813DOI: 10.1093/gbe/evab064ISI: 000661527400011PubMedID: 33760095OAI: oai:DiVA.org:uu-448813DiVA, id: diva2:1579520
Funder
Swedish Research Council, VR 20134508Swedish Research Council, VR 2019-04791Swedish Research Council, 2018-05973Swedish National Infrastructure for Computing (SNIC)Available from: 2021-07-09 Created: 2021-07-09 Last updated: 2025-02-01Bibliographically approved

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Boman, JesperMugal, Carina F.Backström, Niclas

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