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Year-round production of forest seedlings under LED lamps: Biological and energetic implications of indoor cultivation
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Materials Science and Engineering, Solar Cell Technology.ORCID iD: 0000-0001-6805-2112
2021 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Year-round cultivation of forest seedlings under light emitting diodes (LED) is a technology receiving increased attention from nurseries in the boreal forest regions. In these areas, the climate is characterized by strong seasonal fluctuations, resulting in short vegetation periods with narrow time windows for seedling transplanting and outdoor growth. An indoor pre-cultivation phase under LEDs would offer nurseries the possibility to extend their production throughout the year.

LED lamps present several advantages compared to traditional light sources such as higher efficacies, longer lifetimes and advanced controls. In order to test their feasibility for seedling cultivation, three different LED lamps were compared against fluorescent lights. Biological effects of the light quality were studied through pre-cultivating seedlings of Picea abies (L.) Karst. and Pinus sylvestris L. under each spectrum in a growth room with controlled conditions. LEDs showed equal or better results than the control also after a forest field trial.

Effects of light quantity were studied for both species using the most promising LED spectrum. Unlike the energy input, which can be considered proportional to the photon flux output, seedling growth exhibited a non-linear behavior with an optimum light intensity depending on the specific requirements of each species. 

The year-round cultivation concept enables seedlings to be produced outside of the vegetation period. However, these batches need to be cold stored until the transplanting window opens. Thus, protocols to induce cold hardiness in very young seedlings need to be established. Short-day treatments comparing different temperatures and photoperiods using LED lamps were investigated. Lower temperatures had a significant effect on inducing cold hardiness, especially for Pinus sylvestris seedlings.

In contrast to some horticultural crops, which can be cultivated entirely under LEDs, forest seedlings need to be transplanted outdoors at a very young age. This poses a risk for photodamage due to high sunlight intensity and exposure to ultraviolet radiation. Light shock mitigation treatments were investigated by exposing seedlings either to higher LED light intensity, ultraviolet radiation indoors or a transient phase outdoors using shading cloths. Despite some initial stress mitigation, none of the treatments resulted in improved adaptation.

Regardless of how efficient LED lamps become, indoor cultivation will always require electricity. Integrating photovoltaics throughout the nursery could compensate some of this and applying adaptive lighting controls could reduce unnecessary consumption. With proper optimization, these technologies could enable a year-round cultivation under LEDs even in the boreal forest regions.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2021. , p. 108
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 2090
Keywords [en]
Picea abies, Pinus sylvestris, year-round cultivation, forest seedlings, LED lamps, light quality, light quantity, daily light integral, adaptive lighting control, greenhouse integrated photovoltaics, agrivoltaics, seedling growth performance, short-day treatments, cold hardiness, light shock stress
National Category
Energy Systems Forest Science Other Electrical Engineering, Electronic Engineering, Information Engineering
Research subject
Engineering Science
Identifiers
URN: urn:nbn:se:uu:diva-457257ISBN: 978-91-513-1334-4 (print)OAI: oai:DiVA.org:uu-457257DiVA, id: diva2:1606268
Public defence
2021-12-16, Room B-320, Högskolan Dalarna, Röda vägen 3, Borlänge, 13:15 (English)
Opponent
Supervisors
Funder
EU, FP7, Seventh Framework Programme, 308313Available from: 2021-11-23 Created: 2021-10-27 Last updated: 2021-12-29
List of papers
1. Light quality and intensity of light-emitting diodes during pre-cultivation of Picea abies (L.) Karst. and Pinus sylvestris L. seedlings: impact on growth performance, seedling quality and energy consumption
Open this publication in new window or tab >>Light quality and intensity of light-emitting diodes during pre-cultivation of Picea abies (L.) Karst. and Pinus sylvestris L. seedlings: impact on growth performance, seedling quality and energy consumption
2019 (English)In: Scandinavian Journal of Forest Research, ISSN 0282-7581, E-ISSN 1651-1891, Vol. 34, no 3, p. 159-177Article in journal (Refereed) Published
Abstract [en]

Three different LED lamps with continuous spectra were compared against commonly used fluorescent lights. The lamps were characterized by light output, energy consumption and spectral quality for plant growth. The biological effects of light quality were compared by pre-cultivating seedlings of Picea abies (L.) Karst. and Pinus sylvestris L. under each spectrum for 35 days in a growth chamber with controlled temperature, humidity and photoperiod. The seedlings were then transplanted and cultivated for one vegetation period at the nursery, then planted outdoors on a forest field trial and followed for three years. The seedlings showed similar growth performance for all spectra tested. LED lamps have several advantages to fluorescent light such as energy consumption, longer life span and adjustable light intensity. Regarding light intensity the effects on growth performance were studied for both species using the most promising LED spectra. The photosynthetically active radiation (PAR) was maintained at 50, 100, 200 and 400 mu mol m(-2) s(-1). Unlike energy consumption, seedling development did not display a linear relationship to light intensity. Instead, the results show an optimum light level between 100 and 200 mu mol m(-2) s(-1) for the shade tolerant Picea abies seedlings and a level of around 200 mu mol m(-2) s(-1) for the more shade intolerant Pinus sylvestris seedlings.

Place, publisher, year, edition, pages
Taylor & Francis, 2019
Keywords
Cultivation under LED lamps, growth performance and quality of forest seedlings, light quality, light intensity, energy consumption
National Category
Biological Sciences
Research subject
Research Profiles 2009-2020, Energy and Built Environments
Identifiers
urn:nbn:se:uu:diva-450711 (URN)10.1080/02827581.2019.1578404 (DOI)000459142200001 ()2-s2.0-85061782206 (Scopus ID)
Available from: 2021-08-17 Created: 2021-08-17 Last updated: 2021-11-26Bibliographically approved
2. Treatments for induction of cold hardiness in Picea abies (L.) Karst. and Pinus sylvestris L. seedlings pre-cultivated under light-emitting diodes: impact of photoperiod and temperature including energy consumption and seedling quality after cold storage
Open this publication in new window or tab >>Treatments for induction of cold hardiness in Picea abies (L.) Karst. and Pinus sylvestris L. seedlings pre-cultivated under light-emitting diodes: impact of photoperiod and temperature including energy consumption and seedling quality after cold storage
2020 (English)In: Scandinavian Journal of Forest Research, ISSN 0282-7581, E-ISSN 1651-1891, Vol. 35, no 1-2, p. 46-58Article in journal (Refereed) Published
Abstract [en]

In Nordic climates, the weather allows a narrow time window during spring and summer for forest seedlings to be transplanted from indoor growth to outdoor conditions. If a new method for year-round cultivation under LED lamps is to be successfully introduced in forest nurseries, a cold storage phase for batches produced outside of the vegetation period needs to be included in the concept. Different short-day treatments for induction of cold hardiness in very young seedlings of Picea abies and Pinus sylvestris cultivated solely under LED lamps were investigated. The study compared a combination of two photoperiods (5 h or 8 h) at three different temperatures (5°C, 10°C or 20°C) applied during five weeks. Chlorophyll fluorescence, shoot electrolyte leakage and molecular testing for gene expression of cold acclimation were used to assess the treatments. After a period of three months in cold storage at 2°C, the vitality of the seedlings was evaluated using a root growth capacity test. Lower temperatures during the treatments, especially for Pinus sylvestris, had a significant effect on inducing cold hardiness. The results showed that the photoperiod should not be overly reduced to allow photosynthesis and generation of carbohydrate reserves but it could be optimized to reduce electricity consumption.

Place, publisher, year, edition, pages
Informa UK Limited, 2020
Keywords
Cold hardiness, containerized forest seedlings, photoperiod and temperature, seedling cold storage, seedling quality, short-day treatments, year-round cultivation under LED lamps, Cold storage, Electrolytes, Energy utilization, Gene expression, Light emitting diodes, Plants (botany), Seed, LEd lamps, Forestry
National Category
Agriculture, Forestry and Fisheries
Research subject
Research Profiles 2009-2020, Energy and Built Environments
Identifiers
urn:nbn:se:uu:diva-450632 (URN)10.1080/02827581.2020.1718199 (DOI)000511579400001 ()2-s2.0-85078399653 (Scopus ID)
Available from: 2021-08-17 Created: 2021-08-17 Last updated: 2025-02-07Bibliographically approved
3. Light Shock Stress after Outdoor Sunlight Exposure in Seedlings of Picea abies (L.) Karst. and Pinus sylvestris L. Pre-Cultivated under LEDs: Possible Mitigation Treatments and Their Energy Consumption
Open this publication in new window or tab >>Light Shock Stress after Outdoor Sunlight Exposure in Seedlings of Picea abies (L.) Karst. and Pinus sylvestris L. Pre-Cultivated under LEDs: Possible Mitigation Treatments and Their Energy Consumption
2020 (English)In: Forests, E-ISSN 1999-4907, Vol. 11, no 3, article id 354Article in journal (Refereed) Published
Abstract [en]

Year-round cultivation under light emitting diodes (LEDs) has gained interest in boreal forest regions like Fenno-Scandinavia. This concept offers forest nurseries an option to increase seedling production normally restricted by the short vegetation period and the climate conditions. In contrast to some horticultural crops which can be cultivated entirely under LEDs without sunlight, forest seedlings need to be transplanted outdoors in the nursery at a very young age before being outplanted in the field. Juvenile plants are less efficient using absorbed light and dissipating excess energy making them prone to photoinhibition at conditions that usually do not harm mature plants. The outdoor transfer can cause stress in the seedlings due to high sunlight intensity and exposure to ultraviolet (UV) radiation not typically present in the spectra of LED lamps. This study tested possible treatments for mitigating light shock stress in seedlings of Picea abies (L.) Karst. and Pinus sylvestris L. transplanted from indoor cultivation under LEDs to outdoor sunlight exposure. Three sowings were carried out in 2014 (May and June) and 2015 (May) cultivating the seedlings during five weeks under LED lights only. Afterwards, higher light intensity or UV radiation treatments were applied during one week in order to adapt the seedlings to natural outdoor conditions. After transplanting a transition phase was introduced using shading cloths for one or three weeks as outdoor treatments for light shock mitigation. Chlorophyll fluorescence (ChlF) levels and CO2 assimilation rates were measured before transplanting and followed outdoors during 5 weeks. The ChlF results revealed stress symptoms in the photoreceptors during the first days after transplanting. After five weeks outdoors the ChlF levels had recovered and the light saturation points had shifted, allowing higher CO2 assimilation rates. By the end of the vegetation period the morphological attributes showed no major differences between treatments.

Place, publisher, year, edition, pages
MDPI, 2020
Keywords
forest tree seedlings, year-round production, photoinhibition, light-shock, light emitting diodes, light quality and intensity
National Category
Forest Science
Identifiers
urn:nbn:se:uu:diva-413449 (URN)10.3390/f11030354 (DOI)000530221500104 ()
Funder
EU, FP7, Seventh Framework Programme, 308313
Available from: 2020-06-21 Created: 2020-06-21 Last updated: 2024-07-04Bibliographically approved
4. Enabling year-round cultivation in the Nordics: Agrivoltaics and adaptive Led lighting control of Daily Light Integral
Open this publication in new window or tab >>Enabling year-round cultivation in the Nordics: Agrivoltaics and adaptive Led lighting control of Daily Light Integral
2021 (English)In: Agriculture, E-ISSN 2077-0472, Vol. 11, no 12, article id 1255Article in journal (Refereed) Published
Abstract [en]

High efficacy LED lamps combined with adaptive lighting control and greenhouse integrated photovoltaics (PV) could enable the concept of year-round cultivation. This concept can be especially useful for increasing the production in the Nordic countries of crops like herbaceous perennials, forest seedlings, and other potted plants not native of the region, which are grown more than one season in this harsh climate. Meteorological satellite data of this region was analyzed in a parametric study to evaluate the potential of these technologies. The generated maps showed monthly average temperatures fluctuating from −20 °C to 20 °C throughout the year. The natural photoperiod and light intensity also changed drastically, resulting in monthly average daily light integral (DLI) levels ranging from 45–50 mol·m−2·d−1 in summer and contrasting with 0–5 mol·m−2·d−1 during winter. To compensate, growth room cultivation that is independent of outdoor conditions could be used in winter. Depending on the efficacy of the lamps, the electricity required for sole-source lighting at an intensity of 300 µmol·m−2·s−1 for 16 h would be between 1.4 and 2.4 kWh·m−2·d−1. Greenhouses with supplementary lighting could help start the cultivation earlier in spring and extend it further into autumn. The energy required for lighting highly depends on several factors such as the natural light transmittance, the light threshold settings, and the lighting control protocol, resulting in electric demands between 0.6 and 2.4 kWh·m−2·d−1. Integrating PV on the roof or wall structures of the greenhouse could offset some of this electricity, with specific energy yields ranging from 400 to 1120 kWh·kW−1·yr−1 depending on the region and system design.

Place, publisher, year, edition, pages
Springer Nature, 2021
Keywords
daily light integral (DLI) maps, LED grow lights, greenhouse integrated PV, adaptive lighting control, year-round cultivation, agrivoltaics
National Category
Horticulture Forest Science Other Electrical Engineering, Electronic Engineering, Information Engineering
Identifiers
urn:nbn:se:uu:diva-456985 (URN)10.3390/agriculture11121255 (DOI)000735689200001 ()
Projects
FP7 Environment: ZEPHYR - Project ID: 308313
Available from: 2021-10-26 Created: 2021-10-26 Last updated: 2024-01-15Bibliographically approved

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