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Development of a Sandwich ELISA to Quantify the GLP-1 Analogs
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre. Mercodia AB.
2022 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

Immunoassay is a technology to quantify the molecules of biological samples. Enzyme-Linked Immunosorbent Assay (ELISA) is one of the most common immunoassays that is used to analyze biological samples. Mercodia is a company that is specialized in developing immunoassays within the area of metabolic disorders such as type 2 diabetes, obesity, and cardiovascular diseases. Mercodia wanted to develop a prototype sandwich ELISA for quantitative measurement of GLP-1 analogs, especially for Exenatide due to the customer requests. Thus, Mercodia in-house antibodies are paired with anti-Exenatide commercial antibodies and initial tests are performed. Various parameters and conditions are tested. Exenatide and Lixisenatide are successfully detected several times at 10 to 1000 pg/mL concentration. However, further optimizations are required in order to be more consistent with the results and to develop a more sensitive assay.

Place, publisher, year, edition, pages
2022. , p. 59
Keywords [en]
Sandwich ELISA, GLP-1, GLP-1 Analogs, Exenatide, Lixisenatide
National Category
Immunology Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-480062OAI: oai:DiVA.org:uu-480062DiVA, id: diva2:1681245
External cooperation
Mercodia AB
Educational program
Master Programme in Applied Biotechnology
Supervisors
Examiners
Available from: 2022-08-09 Created: 2022-07-06 Last updated: 2022-08-09Bibliographically approved

Open Access in DiVA

The full text will be freely available from 2032-07-29 10:41
Available from 2032-07-29 10:41

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