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Physically and Chemically Crosslinked Hyaluronic Acid-Based Hydrogels Differentially Promote Axonal Outgrowth from Neural Tissue Cultures
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Orthopaedics and Handsurgery.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Orthopaedics and Handsurgery.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Orthopaedics and Handsurgery.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Macromolecular Chemistry.
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2024 (English)In: Biomimetics, E-ISSN 2313-7673, Vol. 9, no 3, article id 140Article in journal (Refereed) Published
Abstract [en]

Our aim was to investigate axonal outgrowth from different tissue models on soft biomaterials based on hyaluronic acid (HA). We hypothesized that HA-based hydrogels differentially promote axonal outgrowth from different neural tissues. Spinal cord sliced cultures (SCSCs) and dorsal root ganglion cultures (DRGCs) were maintained on a collagen gel, a physically crosslinked HA-based hydrogel (Healon 5®) and a novel chemically crosslinked HA-based hydrogel, with or without the presence of neurotrophic factors (NF). Time-lapse microscopy was performed after two, five and eight days, where axonal outgrowth was assessed by automated image analysis. Neuroprotection was investigated by PCR. Outgrowth was observed in all groups; however, in the collagen group, it was scarce. At the middle timepoint, outgrowth from SCSCs was superior in both HA-based groups compared to collagen, regardless of the presence of NF. In DRGCs, the outgrowth in Healon 5® with NF was significantly higher compared to the rest of the groups. PCR revealed upregulation of NeuN gene expression in the HA-based groups compared to controls after excitotoxic injury. The differences in neurite outgrowth from the two different tissue models suggest that axons differentially respond to the two types of biomaterials.

Place, publisher, year, edition, pages
MDPI, 2024. Vol. 9, no 3, article id 140
Keywords [en]
axonal outgrowth, neuroprotection, HA-based hydrogel, spinal cord slice culture, dorsal root ganglion culture
National Category
Cell Biology Neurosciences Biomaterials Science
Identifiers
URN: urn:nbn:se:uu:diva-526176DOI: 10.3390/biomimetics9030140ISI: 001191411300001PubMedID: 38534825OAI: oai:DiVA.org:uu-526176DiVA, id: diva2:1850175
Part of project
Engineering Gene Activated Biomaterial to Induce Bone Formation, Swedish Research Council
Funder
Swedish Research Council, 2020-04025Region UppsalaUppsala UniversityPromobilia foundationEU, Horizon 2020, 955335Swedish Foundation for Strategic Research, 2009-1035Available from: 2024-04-09 Created: 2024-04-09 Last updated: 2024-04-09Bibliographically approved

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Bajic, AndrejAndersson, BrittmarieOssinger, AlexanderTavakoli, ShimaVarghese, Oommen P.Schizas, Nikos

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