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(English)Manuscript (preprint) (Other academic)
Abstract [en]
Molecular changes in the fallopian tubes (FT) play a crucial role in the development of cancer and reproductive disorders. Here, we aimed to map key FT proteins on the single-cell level utilizing an integrated transcriptomics and proteomics approach. Based on RNA-seq, 310 genes were identified as elevated in FT, out of which a majority were associated with motile cilia function. An in-depth spatial characterization was performed for 133 of these genes in FT and other human tissues with motile cilia, localizing the proteins to different subcellular structures of ciliated cells. The specificity for ciliated cells was validated with single-cell RNA-seq and spatial mass-spectrometry data. Our approach enabled us to identify 44 novel cilia-related proteins lacking previous evidence on the protein level, as well as several other proteins not described in the context of cilia biology. The high-resolution spatial map aids in further disentangling pathways involved in infertility and diseases linked to cilia-specific functions.
Keywords
motile cilia, fallopian tube, transcriptomics, spatial proteomics
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-538405 (URN)
2024-09-152024-09-152024-09-15