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Pooled optical screening in bacteria using chromosomally expressed barcodes
Stockholm Univ, Dept Biochem & Biophys, SciLifeLab Stockholm, Stockholm, Sweden..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology. Uppsala University, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0003-2849-8528
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Systems Biology. Uppsala University, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-7355-9751
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Systems Biology.
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2025 (English)In: Communications Biology, E-ISSN 2399-3642, Vol. 8, no 1, article id 851Article in journal (Refereed) Published
Abstract [en]

Optical pooled screening is an important tool to study dynamic phenotypes for libraries of genetically engineered cells. However, the desired engineering often requires that the barcodes used for in situ genotyping are expressed from the chromosome. This has not previously been achieved in bacteria. Here we describe a method for in situ genotyping of libraries with genomic barcodes in Escherichia coli. The method is applied to measure the intracellular maturation time of 84 red fluorescent proteins.
Place, publisher, year, edition, pages
Springer Nature, 2025. Vol. 8, no 1, article id 851
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Molecular Biology Biophysics
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URN: urn:nbn:se:uu:diva-559319DOI: 10.1038/s42003-025-08268-5ISI: 001501519500003PubMedID: 40461651OAI: oai:DiVA.org:uu-559319DiVA, id: diva2:1969788
Funder
EU, European Research CouncilSwedish Research Council, 2018-03958Swedish Research Council, 2019-01238Swedish Research Council, 2018-05973Knut and Alice Wallenberg Foundation, 2016.0077Knut and Alice Wallenberg Foundation, 2017.0291Knut and Alice Wallenberg Foundation, 2019.0439Available from: 2025-06-16 Created: 2025-06-16 Last updated: 2025-06-16Bibliographically approved

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Garcia-Soriano, Daniela A.Larsson, JimmyFange, DavidSirman, DvirKnöppel, AnnaSen, Beer ChakraZikrin, SpartakElf, Johan

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Garcia-Soriano, Daniela A.Larsson, JimmyFange, DavidSirman, DvirKnöppel, AnnaSen, Beer ChakraSvahn, FabianZikrin, SpartakElf, Johan
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