Logo: to the web site of Uppsala University

uu.sePublications from Uppsala University
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Search for the Argonaute protein that governs miRNA regulation in Dictyostelium discoideum
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2021 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

MicroRNAs are small non-coding RNAs that regulate gene expression through RNA interference. These small RNAs enact gene silencing by forming a RNA-inducing silencing complex together with the effector protein Argonaute. The function of the Argonautes in the social amoeba Dictyostelium discoideum is not yet fully understood. In this study, we look closer at Argonaute B by investigating if it is possible to extract the protein from the cells by the addition of a polypeptide protein tag called 3xFlag. At the same time, we also look into if Argonaute B is important for cell growth. Sequences of the 3xFlag tag with or without the Argonaute B gene (agnB) attached had previously been cloned into a vector and transformed into Dictyostelium discoideum cell. The 3xFlag::agnB sequence was confirmed in wild type and agnB knock-out strains through polymerase chain reaction. We then verified the expression of the fusion protein in the cells by western blot. The cell growth was measured by how the number of cells changed over time. The experiment suggested that Argonaute B is important for growth. Our result show that the construct 3xFlag::agnB sequenced had correctly been transformed into the strains and is highly expressed under tested conditions. We could also see that Argonaute B is an important factor in cell growth.

Place, publisher, year, edition, pages
2021. , p. 18
Keywords [en]
Argonaute proteins, RNA interference, miRNA, 3xFLAG-tag
National Category
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-432967OAI: oai:DiVA.org:uu-432967DiVA, id: diva2:1522179
Educational program
Bachelor Programme in Biology / Molecular Biology
Supervisors
Examiners
Available from: 2021-02-04 Created: 2021-01-25 Last updated: 2021-02-04Bibliographically approved

Open Access in DiVA

fulltext(880 kB)66 downloads
File information
File name FULLTEXT01.pdfFile size 880 kBChecksum SHA-512
cfc2e3f2a9ee191c5c7d80d1846475afd959d3908c2535ce5393740fd2858bd9b80ff24cb12f31032dd5f4165f6c98dcc5b2700c24fe3daca1c4043dcf3ff6f2
Type fulltextMimetype application/pdf

By organisation
Biology Education Centre
Microbiology

Search outside of DiVA

GoogleGoogle Scholar
Total: 66 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 140 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf